﻿               SEQUENCE LISTING

<110> Charité - Universitaetsmedizin Berlin
      Max-Delbrueck-Centrum fuer Molekulare Medizin

<120> CD-22-specific T cell receptors and adoptive T cell therapy for treatment of B cell malignancies

<130> 11780 P 6581 EP

<150> EP18213482.5

<160> 27

<170> BiSSAP 1.3.6

<210> 1
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> FLS peptide: CD22 epitope

<400> 1
Phe Leu Ser Asn Asp Thr Val Gln Leu 
1               5                   

<210> 2
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> alpha chain CDR1

<400> 2
Val Thr Asn Phe Arg Ser 
1               5       

<210> 3
<211> 7
<212> PRT
<213> Homo sapiens


<220> 
<223> alpha chain CDR2

<400> 3
Leu Thr Ser Ser Gly Ile Glu 
1               5           

<210> 4
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> alpha chain CDR3

<400> 4
Cys Ala Val Asp Asn Gln Gly Gly Lys Leu Ile Phe 
1               5                   10          

<210> 5
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> beta chain CDR1

<400> 5
Asp Phe Gln Ala Thr Thr 
1               5       

<210> 6
<211> 7
<212> PRT
<213> Homo sapiens


<220> 
<223> beta chain CDR2

<400> 6
Ser Asn Glu Gly Ser Lys Ala 
1               5           

<210> 7
<211> 14
<212> PRT
<213> Homo sapiens


<220> 
<223> beta chain CDR3

<400> 7
Cys Ser Ala Arg Pro Ser Gly Val Tyr Asn Glu Gln Phe Phe 
1               5                   10                  

<210> 8
<211> 411
<212> DNA
<213> Artificial Sequence


<220> 
<223> murine constant region of TCR alpha - codon-optimized

<400> 8
aacatccaga accccgagcc cgccgtgtac cagctgaagg accccagaag ccaggacagc      60

accctgtgcc tgttcaccga cttcgacagc cagatcaacg tgcccaagac catggaaagc     120

ggcaccttca tcaccgacaa gacagtgctg gacatgaagg ccatggacag caagagcaac     180

ggcgccattg cctggtccaa ccagaccagc ttcacatgcc aggacatctt caaagagaca     240

aacgccacct accccagcag cgacgtgccc tgcgacgcca ccctgaccga gaagagcttc     300

gagacagaca tgaacctgaa tttccagaac ctgagcgtga tgggcctgcg gatcctgctg     360

ctgaaggtgg ccggcttcaa cctgctgatg accctgcggc tgtggagcag c              411


<210> 9
<211> 423
<212> DNA
<213> Artificial Sequence


<220> 
<223> minimally murinized constant region - TCR alpha

<400> 9
aacatccaga accccgaccc cgccgtgtat cagctgagag acagcaagag cagcgacaag      60

tccgtgtgcc tgttcaccga cttcgacagc cagaccaatg tgtcccagtc caaggacagc     120

gacgtgtaca tcaccgacaa gaccgtgctg gacatgcgca gcatggactt caagagcaac     180

tccgccgtgg cctggtccaa caagagcgat ttcgcctgcg ccaacgcctt caacaacagc     240

attatccccg aggacacatt cttcccaagc tcagacgttc cctgcgacgt gaagctggtg     300

gaaaagagct tcgagacaga caccaacctg aacttccaga acctgagcgt gatcggcttc     360

cggatcctgc tgctgaaggt ggccggcttc aacctgctga tgaccctgag actgtggtcc     420

agc                                                                   423


<210> 10
<211> 423
<212> DNA
<213> Artificial Sequence


<220> 
<223> human constant region TCR alpha - codon-optimized

<400> 10
aacatccaga accccgaccc cgccgtgtat cagctgagag acagcaagag cagcgacaag      60

tccgtgtgcc tgttcaccga cttcgacagc cagaccaatg tgtcccagtc caaggacagc     120

gacgtgtaca tcaccgacaa gaccgtgctg gacatgcgca gcatggactt caagagcaac     180

tccgccgtgg cctggtccaa caagagcgat ttcgcctgcg ccaacgcctt caacaacagc     240

attatccccg aggacacatt cttcccaagc cccgagagca gctgcgacgt gaagctggtg     300

gaaaagagct tcgagacaga caccaacctg aacttccaga acctgagcgt gatcggcttc     360

cggatcctgc tgctgaaggt ggccggcttc aacctgctga tgaccctgag actgtggtcc     420

agc                                                                   423


<210> 11
<211> 519
<212> DNA
<213> Artificial Sequence


<220> 
<223> murine constant region TCR beta - codon optimized

<400> 11
gaagatctga ggaacgtgac cccacccaag gtgtccctgt tcgagcccag caaggccgag      60

atcgccaaca agcagaaagc caccctggtc tgcctggcca ggggcttctt ccccgaccac     120

gtggagctgt cttggtgggt gaacggcaaa gaggtgcaca gcggagtcag taccgacccc     180

caggcctaca aagagagcaa ctacagctac tgcctgagca gcaggctgag agtgagcgcc     240

accttctggc acaacccccg gaaccacttc cggtgccagg tgcagttcca cggcctgagc     300

gaagaggaca agtggcctga gggcagcccc aagcccgtga cccagaacat cagcgccgag     360

gcctggggca gagccgactg cggcatcacc agcgccagct accaccaggg cgtgctgtcc     420

gccaccatcc tgtacgagat cctgctgggc aaggccaccc tgtacgccgt gctggtgtcc     480

ggcctggtgc tgatggccat ggtgaagaag aagaacagc                            519


<210> 12
<211> 537
<212> DNA
<213> Artificial Sequence


<220> 
<223> minimally murinized constant region TCR beta codon-optimized

<400> 12
gaggatctga agaacgtgtt ccccccagag gtggccgtgt tcgagccttc taaagccgag      60

atcgcacaca cccagaaagc caccctcgtg tgtctggcca ccggcttcta ccccgaccat     120

gtggaactgt cttggtgggt caacggcaaa gaggtgcaca gcggagtgtc caccgacccc     180

cagcctctga aagaacagcc cgccctgaac gacagccggt actgcctgag cagcagactg     240

agagtgtccg ccaccttctg gcagaacccc cggaaccact tcagatgcca ggtgcagttc     300

tacggcctga gcgagaacga cgagtggacc caggacagag ccaagcccgt gacccagatc     360

gtgtctgccg aagcctgggg cagagccgat tgcggcatca ccagcgcaag ctaccatcag     420

ggcgtgctga gcgccacaat cctgtacgag attctgctgg gcaaggccac actgtacgcc     480

gtgctggtgt ctgccctggt gctgatggcc atggtcaagc ggaaggacag cagaggc        537


<210> 13
<211> 537
<212> DNA
<213> Artificial Sequence


<220> 
<223> human constant region TCR beta - codon-optimized

<400> 13
gaggatctga agaacgtgtt ccccccagag gtggccgtgt tcgagccttc tgaggccgag      60

atcagccaca cccagaaagc caccctcgtg tgtctggcca ccggcttcta ccccgaccat     120

gtggaactgt cttggtgggt caacggcaaa gaggtgcaca gcggagtgtc caccgacccc     180

cagcctctga aagaacagcc cgccctgaac gacagccggt actgcctgag cagcagactg     240

agagtgtccg ccaccttctg gcagaacccc cggaaccact tcagatgcca ggtgcagttc     300

tacggcctga gcgagaacga cgagtggacc caggacagag ccaagcccgt gacccagatc     360

gtgtctgccg aagcctgggg cagagccgat tgcggcttta ccagcgagag ctaccagcag     420

ggcgtgctga gcgccacaat cctgtacgag attctgctgg gcaaggccac actgtacgcc     480

gtgctggtgt ctgccctggt gctgatggcc atggtcaagc ggaaggacag cagaggc        537


<210> 14
<211> 390
<212> DNA
<213> Homo sapiens


<220> 
<223> aCD22 TCR alpha chain variable region TRAV36/DV7*04-TRAJ23*01
      incl. leader

<400> 14
atgaagtgtc cacaggcttt actagctatc ttttggcttc tactgagctg ggtgagcagt      60

gaagacaagg tggtacaaag ccctctatct ctggttgtcc acgagggaga cactgtaact     120

ctcaattgca gttatgaagt gactaacttt cgaagcctac tatggtacaa gcaggaaaag     180

aaagctccca catttctatt tatgctaact tcaagtggaa ttgaaaagaa gtcaggaaga     240

ctaagtagca tattagataa gaaagaactt ttcagcatcc tgaacatcac agccacccag     300

accggagact cggccgtcta cctctgtgct gtggataacc agggaggaaa gcttatcttc     360

ggacagggaa cggagttatc tgtgaaaccc                                      390


<210> 15
<211> 390
<212> DNA
<213> Artificial Sequence


<220> 
<223> aCD22 TCR alpha chain variable region TRAV36/DV7*04-TRAJ23*01 -
      codon-optimized

<400> 15
atgaagtgcc cacaggccct gctggccatc ttttggctgc tgctgagctg ggtgtccagc      60

gaggacaagg tggtgcagag ccctctgagc ctggtggtgc acgagggcga taccgtgacc     120

ctgaattgca gctacgaagt gaccaacttc cggtccctgc tgtggtacaa gcaggaaaag     180

aaggccccca ccttcctgtt catgctgacc agcagcggca tcgagaagaa gtccggcaga     240

ctgtcctcca tcctggacaa gaaagagctg ttcagcatcc tgaatatcac cgccacccag     300

accggcgaca gcgccgtgta tctgtgcgcc gtggataacc agggcggcaa gctgatcttt     360

ggccagggca cagagctgag cgtgaagccc                                      390


<210> 16
<211> 130
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..20
<223> leader

<220> 
<223> aDC22 TCR alpha chain variable region TRAV36/DV7*04-TRAJ23*01
      incl. leader

<400> 16
Met Lys Cys Pro Gln Ala Leu Leu Ala Ile Phe Trp Leu Leu Leu Ser 
1               5                   10                  15      
Trp Val Ser Ser Glu Asp Lys Val Val Gln Ser Pro Leu Ser Leu Val 
            20                  25                  30          
Val His Glu Gly Asp Thr Val Thr Leu Asn Cys Ser Tyr Glu Val Thr 
        35                  40                  45              
Asn Phe Arg Ser Leu Leu Trp Tyr Lys Gln Glu Lys Lys Ala Pro Thr 
    50                  55                  60                  
Phe Leu Phe Met Leu Thr Ser Ser Gly Ile Glu Lys Lys Ser Gly Arg 
65                  70                  75                  80  
Leu Ser Ser Ile Leu Asp Lys Lys Glu Leu Phe Ser Ile Leu Asn Ile 
                85                  90                  95      
Thr Ala Thr Gln Thr Gly Asp Ser Ala Val Tyr Leu Cys Ala Val Asp 
            100                 105                 110         
Asn Gln Gly Gly Lys Leu Ile Phe Gly Gln Gly Thr Glu Leu Ser Val 
        115                 120                 125             
Lys Pro 
    130 

<210> 17
<211> 423
<212> DNA
<213> Homo sapiens


<220> 
<223> aCD22 TCR beta chain variable sequence
      TRBV20-1-TRBJ2-1*01-TRBD2*01 incl. leader

<400> 17
atgggggaag gtggtgtgag gccatcacgg aagatgctgc tgcttctgct gcttctgggg      60

ccaggctccg ggcttggtgc tgtcgtctct caacatccga gctgggttat ctgtaagagt     120

ggaacctctg tgaagatcga gtgccgttcc ctggactttc aggccacaac tatgttttgg     180

tatcgtcagt tcccgaaaca gagtctcatg ctgatggcaa cttccaatga gggctccaag     240

gccacatacg agcaaggcgt cgagaaggac aagtttctca tcaaccatgc aagcctgacc     300

ttgtccactc tgacagtgac cagtgcccat cctgaagaca gcagcttcta catctgcagt     360

gctaggccta gcggggtcta caatgagcag ttcttcgggc cagggacacg gctcaccgtg     420

cta                                                                   423


<210> 18
<211> 423
<212> DNA
<213> Artificial Sequence


<220> 
<223> aCD22 TCR beta chain variable region TRBV20-1-TRBJ2-1*01-TRBD2*01
      incl. leader - codon optimized

<400> 18
atgggagaag gcggagtgcg gcctagccgg aagatgcttc tccttctgct gctgctgggc      60

cctggctctg gactgggagc tgtggtgtct cagcacccct cctgggtcat ctgcaagagc     120

ggcaccagcg tgaagatcga gtgcagaagc ctggacttcc aggccaccac catgttctgg     180

tacagacagt tccccaagca gagcctgatg ctgatggcca cctccaacga gggcagcaag     240

gccacatatg agcagggcgt ggaaaaggac aagttcctga tcaaccacgc cagcctgacc     300

ctgagcaccc tgacagtgac aagcgcccac cccgaggaca gcagcttcta catctgcagc     360

gccagaccca gcggcgtgta caacgagcag ttcttcggcc ctggcacccg gctgaccgtg     420

ctg                                                                   423


<210> 19
<211> 141
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..25
<223> Leader sequence aa12-25, TCR sequence starts at aa 26

<220> 
<223> aCD22 TCR beta chain variable region TRBV20-1-TRBJ2-1*01-TRBD2*01
      incl. leader

<400> 19
Met Gly Glu Gly Gly Val Arg Pro Ser Arg Lys Met Leu Leu Leu Leu 
1               5                   10                  15      
Leu Leu Leu Gly Pro Gly Ser Gly Leu Gly Ala Val Val Ser Gln His 
            20                  25                  30          
Pro Ser Trp Val Ile Cys Lys Ser Gly Thr Ser Val Lys Ile Glu Cys 
        35                  40                  45              
Arg Ser Leu Asp Phe Gln Ala Thr Thr Met Phe Trp Tyr Arg Gln Phe 
    50                  55                  60                  
Pro Lys Gln Ser Leu Met Leu Met Ala Thr Ser Asn Glu Gly Ser Lys 
65                  70                  75                  80  
Ala Thr Tyr Glu Gln Gly Val Glu Lys Asp Lys Phe Leu Ile Asn His 
                85                  90                  95      
Ala Ser Leu Thr Leu Ser Thr Leu Thr Val Thr Ser Ala His Pro Glu 
            100                 105                 110         
Asp Ser Ser Phe Tyr Ile Cys Ser Ala Arg Pro Ser Gly Val Tyr Asn 
        115                 120                 125             
Glu Gln Phe Phe Gly Pro Gly Thr Arg Leu Thr Val Leu 
    130                 135                 140     

<210> 20
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> beta chain CDR1

<400> 20
Asp Phe Gln Ala Thr Thr 
1               5       

<210> 21
<211> 7
<212> PRT
<213> Homo sapiens


<220> 
<223> beta chain CDR2

<400> 21
Ser Asn Glu Gly Ser Lys Ala 
1               5           

<210> 22
<211> 14
<212> PRT
<213> Homo sapiens


<220> 
<223> 3225 TCR beta chain CDR3

<400> 22
Cys Ser Ala Arg Glu Ala Gly Val Tyr Asn Glu Gln Phe Phe 
1               5                   10                  

<210> 23
<211> 390
<212> DNA
<213> Homo sapiens


<220> 
<223> 3225 TCR beta chain variable region
      TRBV20-1*01-TRBJ2-1*01-TRBD2*02 F incl. leader

<220> 
<221> sig_peptide
<222> 1..42
<223> /function="leader peptide"

<400> 23
atgctgctgc ttctgctgct tctggggcca ggctccgggc ttggtgctgt cgtctctcaa      60

catccgagct gggttatctg taagagtgga acctctgtga agatcgagtg ccgttccctg     120

gactttcagg ccacaactat gttttggtat cgtcagttcc cgaaacagag tctcatgctg     180

atggcaactt ccaatgaggg ctccaaggcc acatacgagc aaggcgtcga gaaggacaag     240

tttctcatca accatgcaag cctgaccttg tccactctga cagtgaccag tgcccatcct     300

gaagacagca gcttctacat ctgcagtgct agagaagcgg gagtctacaa tgagcagttc     360

ttcgggccag ggacacggct caccgtgcta                                      390


<210> 24
<211> 390
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3225 TCR beta chain variable region codon-optimized
      TRBV20-1*01-TRBJ2-1*01-TRBD2*02 F

<220> 
<221> sig_peptide
<222> 1..42
<223> /function="leader peptide"

<400> 24
atgcttctcc ttctgctgct gctgggccct ggctctggac tgggagctgt ggtgtctcag      60

cacccctcct gggtcatctg caagagcggc accagcgtga agatcgagtg cagaagcctg     120

gacttccagg ccaccaccat gttctggtac agacagttcc ccaagcagag cctgatgctg     180

atggccacct ccaacgaggg cagcaaggcc acatatgagc agggcgtgga aaaggacaag     240

ttcctgatca accacgccag cctgaccctg agcaccctga cagtgacaag cgcccacccc     300

gaggacagca gcttctacat ctgcagcgcc agagaagcgg gcgtgtacaa cgagcagttc     360

ttcggccctg gcacccggct gaccgtgctg                                      390


<210> 25
<211> 130
<212> PRT
<213> Homo sapiens


<220> 
<221> SIGNAL
<222> 1..14
<223> leader peptide

<220> 
<223> 3225 TCR beta chain variable region
      TRBV20-1*01-TRBJ2-1*01-TRBD2*02 F incl. leader

<400> 25
Met Leu Leu Leu Leu Leu Leu Leu Gly Pro Gly Ser Gly Leu Gly Ala 
1               5                   10                  15      
Val Val Ser Gln His Pro Ser Trp Val Ile Cys Lys Ser Gly Thr Ser 
            20                  25                  30          
Val Lys Ile Glu Cys Arg Ser Leu Asp Phe Gln Ala Thr Thr Met Phe 
        35                  40                  45              
Trp Tyr Arg Gln Phe Pro Lys Gln Ser Leu Met Leu Met Ala Thr Ser 
    50                  55                  60                  
Asn Glu Gly Ser Lys Ala Thr Tyr Glu Gln Gly Val Glu Lys Asp Lys 
65                  70                  75                  80  
Phe Leu Ile Asn His Ala Ser Leu Thr Leu Ser Thr Leu Thr Val Thr 
                85                  90                  95      
Ser Ala His Pro Glu Asp Ser Ser Phe Tyr Ile Cys Ser Ala Arg Glu 
            100                 105                 110         
Ala Gly Val Tyr Asn Glu Gln Phe Phe Gly Pro Gly Thr Arg Leu Thr 
        115                 120                 125             
Val Leu 
    130 

<210> 26
<211> 42
<212> DNA
<213> Homo sapiens


<220> 
<223> 3225 beta chain construct CDR3 nt

<400> 26
tgcagtgcta gagaagcggg agtctacaat gagcagttct tc                        42


<210> 27
<211> 42
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3225 beta chain construct CDR3 nt codon optimized

<400> 27
tgcagcgcca gagaagcggg cgtgtacaac gagcagttct tc                        42


