﻿               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> PHAGE RESISTANT LACTIC ACID BACTERIA


<130> 32912-WO-PCT

<150> EP18213374.4
<151> 2018-12-18

<160> 8

<170> BiSSAP 1.3.6

<210> 1
<211> 3394
<212> DNA
<213> Artificial Sequence


<220> 
<223> High copy plasmid with Lactococcus P44 promoter

<400> 1
taacaattgt aacccatcca ggagacggga cgatagcaat tttttcaata agtagacaaa       60

gtagagaata atttaataaa aaactgaaaa aatcacaggt aaactcttgt tttactgtga      120

ttttatgtta aaataattaa tgagtgtaat tgtatataaa attatctgta cacttaccta      180

atttattgaa aaaaatatga atcgtgatgt gtgagggaaa ggaggcgctt ttatggccaa      240

accatgggtc ctgcaggctc gcggtaccat tagttctaga gagctcaagc tttctttgaa      300

ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa tggcaattaa acaaattaca      360

gcacgtgttg ctttgattga tagccaaaaa gcagcagttg ataaagcaat tactgatatt      420

gctgaaaaat tgtaatttat aaataaaaat caccttttag aggtggtttt tttatttata      480

aattattcgt ttgatttcgc tttcgataga acaatcaaag cgagaataag gaagataaat      540

cccataaggg cgggagcaga atgtccgaga ctaatgtaaa tttgtccacc aattaaagga      600

ccgataacgc gagcttctcg agtgcatatt ttcggcaatc ttctcaatga gatgctcttc      660

agcatgttca atgatgtcga ttttttatta aaacgtctca aaatcgtttc tgagacgttt      720

tagcgtttat ttcgtttagt tatcggcata atcgttaaaa caggcgttat cgtagcgtaa      780

aagcccttga gcgtagcgtg ctttgcagcg aagatgttgt ctgttagatt atgaaagccg      840

atgactgaat gaaataataa gcgcagcgtc cttctatttc ggttggagga ggctcaaggg      900

agtttgaggg aatgaaattc cctcatgggt ttgattttaa aaattgcttg caattttgcc      960

gagcggtagc gctggaaaat ttttgaaaaa aatttggaat ttggaaaaaa atggggggaa     1020

aggaagcgaa ttttgcttcc gtactacgac cccccattaa gtgccgagtg ccaatttttg     1080

tgccaaaaac gctctatccc aactggctca agggtttgag gggtttttca atcgccaacg     1140

aatcgccaac gttttcgcca acgtttttta taaatctata tttaagtagc tttattgttg     1200

tttttatgat tacaaagtga tacactaatt ttataaaatt atttgattgg agttttttaa     1260

atggtgattt cagaatcgaa aaaaagagtt atgatttctc tgacaaaaga gcaagataaa     1320

aaattaacag atatggcgaa acaaaaaggt ttttcaaaat ctgcggttgc ggcgttagct     1380

atagaagaat atgcaagaaa ggaatcagaa caaaaaaaat aagcgaaagc tcgcgttttt     1440

agaaggatac gagttttcgc tacttgtttt tgataaggta atatatcatg gctattaaaa     1500

atactaaagc tagaaatttt ggatttttat tatatcctga ctcaattcct aatgattgga     1560

aagaaaaatt agagagtttg ggcgtatcta tggctgtcag tcctttacac gatatggacg     1620

aaaaaaaaga taaagataca tggaatagta gtgatgttat acgaaatgga aagcactata     1680

aaaaaccaca ctatcacgtt atatatattg cacgaaatcc tgtaacaata gaaagcgtta     1740

ggaacaagat taagcgaaaa ttggggaata gttcagttgc tcatgttgag atacttgatt     1800

atatcaaagg ttcatatgaa tatttgactc atgaatcaaa ggacgctatt gctaagaata     1860

aacatatata cgacaaaaaa gatattttga acattaatga ttttgatatt gaccgctata     1920

taacacttga tgaaagccaa aaaagagaat tgaagaattt acttttagat atagtggatg     1980

actataattt ggtaaataca aaagatttaa tggcttttat tcgccttagg ggagcggagt     2040

ttggaatttt aaatacgaat gatgtaaaag atattgtttc aacaaactct agcgccttta     2100

gattatggtt tgagggcaat tatcagtgtg gatatagagc aagttatgca aaggttcttg     2160

atgctgaaac gggggaaata aaatgacaaa caaagaaaaa gagttatttg ctgaaaatga     2220

ggaattaaaa aaagaaatta aggacttaaa agagcgtatt gaaagataca gagaaatgga     2280

agttgaatta agtacaacaa tagatttatt gagaggaggg attattgaat aaataaaagc     2340

ccccctgacg aaagtcgacg gcaatagtta cccttattat caagataaga aagaaaagga     2400

tttttcgcta cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc     2460

tttattcttc aactaaagca cccattagtt caacaaacga aaattggata aagtgggata     2520

tttttaaaat atatatttat gttacagtaa tattgacttt taaaaaagga ttgattctaa     2580

tgaagaaagc agacaagtaa gcctcctaaa ttcactttag ataaaaattt aggaggcata     2640

tcaaatgaac tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca     2700

ttatttgaac caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata     2760

ccgaaacata aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag     2820

ggtgataaac tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta     2880

ttgggataag ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg     2940

tatttggact cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt     3000

agagaaatat aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt     3060

ttctctttct attattcctt ggacttcatt tactgggttt aacttaaata tcaataataa     3120

tagtaattac cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat     3180

atatttaccg ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt     3240

gtttatgaac tctattcagg aattgtcaga taggcctaat gactggcttt tataatatga     3300

gataatgccg actgtacttt ttacagtcgg ttttctaatg tcactaacct gccccgttag     3360

ttgaagaagg tttttatatt acagctccaa gatc                                 3394


<210> 2
<211> 226
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of putative anti-CRISPR protein found in φ100E-D1
      with overlap to pNZ44

<400> 2
accatgggtc ctgcaggctc gcggtaccat tagttatgaa atataaagta attacatatt     60

ttgacaacat ggaagatgat gtagaagtat ttgacagaaa ggatgaagct attaacagat    120

tgcatcatct acgaggtgtt aaatatagaa attcaagatt atataaagta gaaatggttg    180

aggtggaata gagagctcaa gctttctttg aaccaaaatt agaaaa                   226


<210> 3
<211> 226
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of putative anti-CRISPR protein found in φ200F-D77
      with overlap to pNZ44

<400> 3
accatgggtc ctgcaggctc gcggtaccat tagttatgag atataaagtt attgtttatt     60

acgacaatat ggaagacgat gtagaaattt atgagaataa ggatgaagct atcaacagat    120

tgcatcatct acgaggtgtt aaatatagaa attcaagatt atataaagta gaaatggttg    180

aggtggaata aagagctcaa gctttctttg aaccaaaatt agaaaa                   226


<210> 4
<211> 406
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of putative anti-CRISPR protein found in φ300F with
      overlap to pNZ44

<400> 4
accatgggtc ctgcaggctc gcggtaccat tagttatgga agaattagaa caagcgtttg      60

aaaatcttga tgattggtat ctatcaagca tgaaagacag tgcttacaag gattttggga     120

aatacgaaat tcgcttatca aatcattcgg cagacaataa atatcatgac ctagaaaatg     180

gtcgcttaat cgttaatgtt aaagctagta aattgaactt cgttgatatc atcgagaata     240

aaatctataa aatcattgag aaaattgaaa ctctcgattt agacaagtac agatttatta     300

acgctactaa aatggaaaac gatatcaaat gttattacaa gggatttaag acaaagaagg     360

atgtgatcta aagagctcaa gctttctttg aaccaaaatt agaaaa                    406


<210> 5
<211> 493
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of anti-CRISPR protein found by Hynes et al., 2017
      with overlap to pNZ44

<400> 5
accatgggtc ctgcaggctc gcggtaccat tagttatggc atacggaaaa agtagatata      60

actcatatag aaagcgcagt tttaacagaa gtaataagca acgtagagaa tacgcacaag     120

aaatggatag attagagaaa gctttcgaaa atcttgacgg atggtatcta tctagcatga     180

aagacagtgc ttacaaggat tttgggaaat acgaaattcg cttatcaaat cattcggcag     240

acaacaaata tcacgactta gaaaacggtc gtttaattgt taatattaaa gctagtaaat     300

tgaatttcgt tgatatcatc gagaataagc ttgataaaat aatcgagaag attgataagc     360

ttgatttaga taagtaccga ttcatcaatg cgaccaacct agagcatgat atcaaatgct     420

attacaaggg gtttaaaacg aaaaaggagg taatctaaag agctcaagct ttctttgaac     480

caaaattaga aaa                                                        493


<210> 6
<211> 622
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of anti-CRISPR protein found by Hynes et al., 2018,
      with overlap to pNZ44

<400> 6
accatgggtc ctgcaggctc gcggtaccat tagttatgaa aataaatgac gacatcaaag      60

agttaatttt agaatatatg agccgttact tcaaattcga gaacgacttt tataaactgc     120

caggcatcaa gttcactgat gcaaattggc agaagttcaa aaatggaggc actgacattg     180

agaagatggg ggcggcacga gtaaacgcca tgctcgactg cctattcgac gatttcgagc     240

ttgctatgat tggcaaggct caaactaatt attacaatga taattcacta aagatgaaca     300

tgccatttta cacttactat gacatgttca aaaagcagca acttctaaaa tggcttaaaa     360

ataaccgtga tgatgtcatc ggcggaactg gtaggatgta cacagcaagt ggtaattaca     420

ttgctaacgc ttatttagag gtggcattag aatcgagctc gcttggtagt ggctcttaca     480

tgcttcaaat gaggtttaaa gactattcaa aaggtcaaga acctattccg tcaggtcgtc     540

agaatcgact tgaatggatt gaaaacaatc tcgaaaacat tcgataaaga gctcaagctt     600

tctttgaacc aaaattagaa aa                                              622


<210> 7
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> forward primer on backbone pNZ44 vector

<400> 7
accatgggtc ctgcaggct                                                 19


<210> 8
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer on backbone pNZ44 vector

<400> 8
ttttctaatt ttggttcaaa gaaagcttga gctct                                35


