                         SEQUENCE LISTING

<110>  Peking University
       EdiGene Biotechnology Inc.
 
<120>  COMPOSITIONS AND METHODS FOR HIGHLY EFFICIENT GENETIC        SCREENING USING BARCODED GUIDE RNA CONSTRUCTS

<130>  FC00188PCT

<160>  75    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of array-synthesized oligos

<400>  1
ttgtggaaac gtctcaaccg                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of array-synthesized oligos

<400>  2
ctctagctcc gtctcatgtt                                                   20


<210>  3
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For construction of the sgRNAiBAR- expressing backbone

<400>  3
tatattcgaa cgtctctaac agcatagcaa gtttaaataa ggcagtccgt tatcaacttg       60

aaaaa                                                                   65


<210>  4
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For construction of the sgRNAiBAR- expressing backbone

<400>  4
tatactcgag aaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag       60

ccttat                                                                  66


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAsiBAR-ANTXR1 coding region for 
       NGS

<400>  5
aagcggagga caggattggg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAsiBAR-ANTXR1 coding region for 
       NGS

<400>  6
cctctgtggc cctggagatg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in CSPG4 gene

<400>  7
cacgggccct ttaagaaggt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in CSPG4 gene

<400>  8
ggacccactt ctcactgtcg                                                   20


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in MLH1 gene

<400>  9
gtgctcatcg ttgccacata tta                                               23


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in MLH1 gene

<400>  10
tacgtgtaac agacaccttg c                                                 21


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in MSH2 gene

<400>  11
ttgggtgtgg tcgccgtg                                                     18


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in MSH2 gene

<400>  12
cacaagcacc aacgttccg                                                    19


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in MSH6 gene

<400>  13
tttttaaata ctctttcctt gcctg                                             25


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in MSH6 gene

<400>  14
agggcgtttc cttcctagag                                                   20


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in PMS2 gene(sgRNA1,2)

<400>  15
acactgtctt gggaaatgca a                                                 21


<210>  16
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in PMS2 gene(sgRNA1,2)

<400>  16
tggcagcgag acaaaac                                                      17


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in PMS2 gene(sgRNA3)

<400>  17
ctcactgaac acaccatgcc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the T7E1 assay in PMS2 gene(sgRNA3)

<400>  18
ggtctcactg tgttgcccag                                                   20


<210>  19
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  19
tacacgacgc tcttccgatc ttaagtagag tatcttgtgg aaaggacgaa acacc            55


<210>  20
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  20
agacgtgtgc tcttccgatc ttaagtagag agcttatcga taccgtcgac ctc              53


<210>  21
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  21
tacacgacgc tcttccgatc tatcatgctt atatcttgtg gaaaggacga aacacc           56


<210>  22
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  22
agacgtgtgc tcttccgatc tatcatgctt aagcttatcg ataccgtcga cctc             54


<210>  23
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  23
tacacgacgc tcttccgatc tgatgcacat cttatcttgt ggaaaggacg aaacacc          57


<210>  24
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  24
agacgtgtgc tcttccgatc tgatgcacat ctagcttatc gataccgtcg acctc            55


<210>  25
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  25
tacacgacgc tcttccgatc tcgattgctc gactatcttg tggaaaggac gaaacacc         58


<210>  26
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  26
agacgtgtgc tcttccgatc tcgattgctc gacagcttat cgataccgtc gacctc           56


<210>  27
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  27
tacacgacgc tcttccgatc ttcgatagca attctatctt gtggaaagga cgaaacacc        59


<210>  28
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  28
agacgtgtgc tcttccgatc ttcgatagca attcagctta tcgataccgt cgacctc          57


<210>  29
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  29
tacacgacgc tcttccgatc tatcgatagt tgctttatct tgtggaaagg acgaaacacc       60


<210>  30
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  30
agacgtgtgc tcttccgatc tatcgatagt tgcttagctt atcgataccg tcgacctc         58


<210>  31
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  31
tacacgacgc tcttccgatc tgatcgatcc agttagtatc ttgtggaaag gacgaaacac       60

c                                                                       61


<210>  32
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  For PCR amplification of the sgRNAiBAR coding region for NGS

<400>  32
agacgtgtgc tcttccgatc tgatcgatcc agttagagct tatcgatacc gtcgacctc        59


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HPRT1_sgRNA 1

<400>  33
tcaccacgac gccagggctg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HPRT1_sgRNA 2

<400>  34
gttatggcga cccgcagccc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ITGB1_sgRNA 1

<400>  35
acacagcaaa ctgaactgat                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ITGB1_sgRNA 2

<400>  36
tacctgtttg agcaaacaca                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SRGAP2_sgRNA 1

<400>  37
cagccaaatt caaaaaggat                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SRGAP2_sgRNA 2

<400>  38
ccaaattcaa aaaggataag                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AKTIP_sgRNA 1

<400>  39
gcttgtagac atgctccaga                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AKTIP_sgRNA 2

<400>  40
cacgttatga accctttctg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACTR3C_sgRNA 1

<400>  41
caggactcta cattgcagtt                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACTR3C_sgRNA 2

<400>  42
cgttccagga ctctacattg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PPP1R17_sgRNA 1

<400>  43
tgatgtccac tgagcaaatg                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PPP1R17_sgRNA 2

<400>  44
cagtggctgc atttgctcag                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ASCBG1_sgRNA 1

<400>  45
tgggcagccg tatccagctc                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ASCBG1_sgRNA 2

<400>  46
gcagatgcca cgcaattctg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CALM2_sgRNA 1

<400>  47
gtaggctgac caactgactg                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CALM2_sgRNA 2

<400>  48
caatctgctc ttcagtcagt                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCF21_sgRNA 1

<400>  49
actcccccaa acatgtccac                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCF21_sgRNA 2

<400>  50
cacatcgctg agggagccgg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KIFAP3_sgRNA 1

<400>  51
caacacagat ataacttccc                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KIFAP3_sgRNA 2

<400>  52
cagggaagtt atatctgtgt                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FGF13_sgRNA 1

<400>  53
ttgttctctt tgcagagcct                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FGF13_sgRNA 2

<400>  54
tctttgcaga gcctcagctt                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DUPD1_sgRNA 1

<400>  55
cagatgagta ggcattcttg                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DUPD1_sgRNA 2

<400>  56
atgcctactc atctgccaag                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TECTA_sgRNA 1

<400>  57
tgaaagagac ccaaattcta                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TECTA_sgRNA 2

<400>  58
ttcgcacttg tacagcacca                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GALR1_sgRNA 1

<400>  59
ggcggtcggg aacctcagcg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GALR1_sgRNA 2

<400>  60
gttcccgacc gccagctcca                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OR51D1_sgRNA 1

<400>  61
tatgataggg accaagagct                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OR51D1_sgRNA 2

<400>  62
atgataggga ccaagagctg                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLH1_sgRNA 1

<400>  63
attacaacga aaacagctga                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLH1_sgRNA 2

<400>  64
ctgatggaaa gtgtgcatac                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSH2_sgRNA 1

<400>  65
cgcgctgctg gccgcccggg                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSH2_sgRNA 2

<400>  66
ggtcttgaac acctcccggg                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSH2_sgRNA 3

<400>  67
gtgaggaggt ttcgacatgg                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSH6_sgRNA 1

<400>  68
gaagtacagc ctaagacaca                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSH6_sgRNA 2

<400>  69
agcctaagac acaaggatct                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PMS2_sgRNA 1

<400>  70
cgactgatgt ttgatcacaa                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PMS2_sgRNA 2

<400>  71
agtttcaacc tgagttaggt                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSPG4_sgRNA 1

<400>  72
gagttaagtg cgcggacacc                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSPG4_sgRNA 2

<400>  73
ccactcagct cccagctccc                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  neg_sgRNA 1

<400>  74
caatagcaaa ccggggcagt                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  neg_sgRNA 2

<400>  75
gtgactccat taccaggctg                                                   20


