                         SEQUENCE LISTING

<110>  Cambridge Technologies LLC
 
<120>  UNIVERSAL INFLUENZA VACCINE

<130>  51643-PCT

<150>  US 62/778,409
<151>  2018-12-12

<160>  6    

<170>  PatentIn version 3.5

<210>  1
<211>  1410
<212>  DNA
<213>  Influenza A virus


<220>
<221>  CDS
<222>  (1)..(1410)

<220>
<221>  misc_feature
<222>  (1)..(1410)
<223>  A/swine/Iowa/A01782229/2016 (H1N1) neuraminidase

<400>  1
atg aat aca aat caa aga ata ata acc att ggg aca ttt tgc atg ata         48
Met Asn Thr Asn Gln Arg Ile Ile Thr Ile Gly Thr Phe Cys Met Ile           
1               5                   10                  15                

gtt gga ata gtc agt cta ttg tta cag ata gga aac ata gtc tcg tta         96
Val Gly Ile Val Ser Leu Leu Leu Gln Ile Gly Asn Ile Val Ser Leu           
            20                  25                  30                    

tgg att agc cat tca att cag acc gga tgg gaa aat cac act ggg atg        144
Trp Ile Ser His Ser Ile Gln Thr Gly Trp Glu Asn His Thr Gly Met           
        35                  40                  45                        

tgc aac caa agt gtt att aca tat gta aat aac aca tgg gtg aac cga        192
Cys Asn Gln Ser Val Ile Thr Tyr Val Asn Asn Thr Trp Val Asn Arg           
    50                  55                  60                            

act tat gtg aac att agc aat atc aaa att gct act ata cag gat gtg        240
Thr Tyr Val Asn Ile Ser Asn Ile Lys Ile Ala Thr Ile Gln Asp Val           
65                  70                  75                  80            

act ccg att ata cta gcc ggc aat tca cca ctt tgc cca gta agt ggg        288
Thr Pro Ile Ile Leu Ala Gly Asn Ser Pro Leu Cys Pro Val Ser Gly           
                85                  90                  95                

tgg gct gta tac agc aaa gac aat agc ata agg att ggt tct aaa ggg        336
Trp Ala Val Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly           
            100                 105                 110                   

gac att ttt gtc ata aga gaa cca ttc att tca tgc tct caa ttg gaa        384
Asp Ile Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Gln Leu Glu           
        115                 120                 125                       

tgc aga acc ttc ttt ctg acc caa ggt gct ttg ctg aat gac aaa cat        432
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His           
    130                 135                 140                           

tct aat gga acc gtc aag gac agg agt ccc tat aga acc ctg atg agc        480
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser           
145                 150                 155                 160           

tgc ccc atc ggt gaa gcc cca tct ccg tac aac tca agg ttc gaa tca        528
Cys Pro Ile Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser           
                165                 170                 175               

gtt gct tgg tca gca agt gca tgt cat gat ggg atg gga tgg cta aca        576
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr           
            180                 185                 190                   

atc ggg gtc tct ggt cca gat aat gga gca gta gct gtt tta aaa tac        624
Ile Gly Val Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr           
        195                 200                 205                       

aac ggt ata ata aca gat aca ata aaa agt tgg aga aac aaa ata tta        672
Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Lys Ile Leu           
    210                 215                 220                           

aga aca caa gag tca gaa tgt gtt tgt atg aac ggt tct tgt ttt act        720
Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser Cys Phe Thr           
225                 230                 235                 240           

gta tta act gat ggc cca agc aat ggg caa gcc tcg tac aaa ata ttt        768
Val Leu Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe           
                245                 250                 255               

aaa gtg gaa aaa ggg aaa ata att aag tca att gag ctg gat gcc ccc        816
Lys Val Glu Lys Gly Lys Ile Ile Lys Ser Ile Glu Leu Asp Ala Pro           
            260                 265                 270                   

aat tac cac tat gaa gaa tgc tca tgc tat cct gat aca ggc aaa gtt        864
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val           
        275                 280                 285                       

atg tgt gtt tgc aga gac aat tgg cat gcc tcg aac cgg cca tgg gtc        912
Met Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val           
    290                 295                 300                           

tct ttc aat cag aat ctt gac tat caa ata gga tac ata tgc agt gga        960
Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly           
305                 310                 315                 320           

gtt ttc ggt gat aac cct cgt tcc act gat ggg aag ggc aat tgt ggc       1008
Val Phe Gly Asp Asn Pro Arg Ser Thr Asp Gly Lys Gly Asn Cys Gly           
                325                 330                 335               

cca gta ctt tct aat ggg gca aat gga gtg aaa gga ttc tca tat aga       1056
Pro Val Leu Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Arg           
            340                 345                 350                   

tat ggt aat ggt gtt tgg ata gga aga act aag agt atc aac tcc aga       1104
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Asn Ser Arg           
        355                 360                 365                       

agt gga ttt gaa atg att tgg gat cca aat ggg tgg act gaa act gat       1152
Ser Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr Asp           
    370                 375                 380                           

agt agt ttt tct atg aag cag gac att ata gca ttg gat gat tgg tca       1200
Ser Ser Phe Ser Met Lys Gln Asp Ile Ile Ala Leu Asp Asp Trp Ser           
385                 390                 395                 400           

gga tac agt gga agt ttt gtc caa cat ccg gaa tta aca gga atg aat       1248
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Met Asn           
                405                 410                 415               

tgc ata agg cct tgt ttc tgg gtg gag cta atc aga ggg caa ccc aag       1296
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys           
            420                 425                 430                   

gaa agc aca atc tgg gct agc gga agc agc atc tct ttc tgt ggc gta       1344
Glu Ser Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe Cys Gly Val           
        435                 440                 445                       

aat agt gaa acc gca aac tgg tca tgg cca gac ggg gct att ctg cca       1392
Asn Ser Glu Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala Ile Leu Pro           
    450                 455                 460                           

ttc gcc att gac aag tag                                               1410
Phe Ala Ile Asp Lys                                                       
465                                                                       


<210>  2
<211>  469
<212>  PRT
<213>  Influenza A virus

<220>
<221>  MISC_FEATURE
<222>  (1)..(469)
<223>  expressed N1 subtype

<400>  2

Met Asn Thr Asn Gln Arg Ile Ile Thr Ile Gly Thr Phe Cys Met Ile 
1               5                   10                  15      


Val Gly Ile Val Ser Leu Leu Leu Gln Ile Gly Asn Ile Val Ser Leu 
            20                  25                  30          


Trp Ile Ser His Ser Ile Gln Thr Gly Trp Glu Asn His Thr Gly Met 
        35                  40                  45              


Cys Asn Gln Ser Val Ile Thr Tyr Val Asn Asn Thr Trp Val Asn Arg 
    50                  55                  60                  


Thr Tyr Val Asn Ile Ser Asn Ile Lys Ile Ala Thr Ile Gln Asp Val 
65                  70                  75                  80  


Thr Pro Ile Ile Leu Ala Gly Asn Ser Pro Leu Cys Pro Val Ser Gly 
                85                  90                  95      


Trp Ala Val Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly Ser Lys Gly 
            100                 105                 110         


Asp Ile Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Gln Leu Glu 
        115                 120                 125             


Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His 
    130                 135                 140                 


Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser 
145                 150                 155                 160 


Cys Pro Ile Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser 
                165                 170                 175     


Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr 
            180                 185                 190         


Ile Gly Val Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr 
        195                 200                 205             


Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn Lys Ile Leu 
    210                 215                 220                 


Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser Cys Phe Thr 
225                 230                 235                 240 


Val Leu Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe 
                245                 250                 255     


Lys Val Glu Lys Gly Lys Ile Ile Lys Ser Ile Glu Leu Asp Ala Pro 
            260                 265                 270         


Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val 
        275                 280                 285             


Met Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg Pro Trp Val 
    290                 295                 300                 


Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile Cys Ser Gly 
305                 310                 315                 320 


Val Phe Gly Asp Asn Pro Arg Ser Thr Asp Gly Lys Gly Asn Cys Gly 
                325                 330                 335     


Pro Val Leu Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Tyr Arg 
            340                 345                 350         


Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Asn Ser Arg 
        355                 360                 365             


Ser Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Glu Thr Asp 
    370                 375                 380                 


Ser Ser Phe Ser Met Lys Gln Asp Ile Ile Ala Leu Asp Asp Trp Ser 
385                 390                 395                 400 


Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Met Asn 
                405                 410                 415     


Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Gln Pro Lys 
            420                 425                 430         


Glu Ser Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe Cys Gly Val 
        435                 440                 445             


Asn Ser Glu Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala Ile Leu Pro 
    450                 455                 460                 


Phe Ala Ile Asp Lys 
465                 


<210>  3
<211>  1410
<212>  DNA
<213>  Influenza A virus


<220>
<221>  CDS
<222>  (1)..(1410)

<220>
<221>  misc_feature
<222>  (1)..(1410)
<223>  A/swine/Oklahoma/A01730659/2016 (H1N2) neuraminidase

<400>  3
atg aat cca aat caa aag ata ata aca att ggc tct gtt tct ctc atc         48
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Ile           
1               5                   10                  15                

att gcc aca ata tgc ttc ctt atg caa att gcc atc ctg gtg act act         96
Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr           
            20                  25                  30                    

gta aca ctg cat ttc aag caa cat aat tgc gac tcc tcc cca aac aac        144
Val Thr Leu His Phe Lys Gln His Asn Cys Asp Ser Ser Pro Asn Asn           
        35                  40                  45                        

cat gta atg ttt tgt gaa cca aca ata ata gaa aga aac aaa acg gag        192
His Val Met Phe Cys Glu Pro Thr Ile Ile Glu Arg Asn Lys Thr Glu           
    50                  55                  60                            

att gtg tat ctg acc aac acc act gta gag aag gaa ata tgc ccc aaa        240
Ile Val Tyr Leu Thr Asn Thr Thr Val Glu Lys Glu Ile Cys Pro Lys           
65                  70                  75                  80            

tca aca gaa tac aga aat tgg tca aag cct caa tgt aac att aca gga        288
Ser Thr Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly           
                85                  90                  95                

ttt gca cct ttt tct aag gac aat tcg att cgg ctt tct gct ggt ggg        336
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly           
            100                 105                 110                   

gac atc tgg gtg aca aga gaa cct tat gtg tca tgc gat cac gac aag        384
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp His Asp Lys           
        115                 120                 125                       

tgt tat caa ttt gcc ctt ggg cag gga aca aca cta aac aac ggg cat        432
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His           
    130                 135                 140                           

tca aat gac act gta cat gat agg acc cct tac cga acc cta ttg atg        480
Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met           
145                 150                 155                 160           

aat gaa ttg ggg gtt cca ttt cat ttg gga acc agg caa gtg tgc ata        528
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile           
                165                 170                 175               

gca tgg tcc agt tca agt tgt cac gat ggg aaa gca tgg ctg cat gtt        576
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val           
            180                 185                 190                   

tgt ata act ggg gat gat gaa aat gca act gct agc ttc att tac aat        624
Cys Ile Thr Gly Asp Asp Glu Asn Ala Thr Ala Ser Phe Ile Tyr Asn           
        195                 200                 205                       

ggg agg cta gta gat agt att ggt tca tgg tcc aaa aat ata cta aga        672
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn Ile Leu Arg           
    210                 215                 220                           

acc cag gag tcg gaa tgc gtt tgt att aat gga act tgt aca gta gtc        720
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val           
225                 230                 235                 240           

atg act gat gga agc gct tcc gga aaa gct gat act aaa ata tta ttc        768
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe           
                245                 250                 255               

att gag gag ggg aaa atc att cat att agc acg ttg tca gga agt gcg        816
Ile Glu Glu Gly Lys Ile Ile His Ile Ser Thr Leu Ser Gly Ser Ala           
            260                 265                 270                   

cag cac gta gag gag tgc tct tgt tat cct cga tat cct ggt gtc aga        864
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg           
        275                 280                 285                       

tgt gtc tgc aga gac aac tgg aaa ggc tcc aat agg ccc ata gtt gat        912
Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp           
    290                 295                 300                           

ata aat gta aag gat tat agc act gtt tcc agt tat gta tgc tct gga        960
Ile Asn Val Lys Asp Tyr Ser Thr Val Ser Ser Tyr Val Cys Ser Gly           
305                 310                 315                 320           

ctt gtt gga gac aca ccc aga aaa aac gac agc ttc agc agt agt aat       1008
Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Phe Ser Ser Ser Asn           
                325                 330                 335               

tgc cta gac cct aat aat gag gaa ggt ggt cat ggg gta aaa ggt tgg       1056
Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp           
            340                 345                 350                   

gcc ttt gat gat gga gat gac ttg tgg atg gga aga acg atc agc gag       1104
Ala Phe Asp Asp Gly Asp Asp Leu Trp Met Gly Arg Thr Ile Ser Glu           
        355                 360                 365                       

aag tca cgt tta ggc tat gaa acc ttc aaa gtc gtc aaa gga tgg tcc       1152
Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Val Lys Gly Trp Ser           
    370                 375                 380                           

aaa ccc aac tcc aaa tta cag aca aat agg caa gtt ata gtt gat aga       1200
Lys Pro Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Asp Arg           
385                 390                 395                 400           

ggt aac agg tcc ggt tat tct ggt att ttc tcc att gaa ggc aaa aac       1248
Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Ile Glu Gly Lys Asn           
                405                 410                 415               

tgt att aat agg tgc ttt tat gtg gag ttg ata agg gga agg aaa gag       1296
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu           
            420                 425                 430                   

gaa act aaa gtc ttg tgg acc tca aac agt att gtt gtg ttt tgt ggc       1344
Glu Thr Lys Val Leu Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly           
        435                 440                 445                       

acc tca ggt acg tat gga aca ggc tca tgg cct gat ggg gcg gat atc       1392
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile           
    450                 455                 460                           

aat ctc atg cct ata taa                                               1410
Asn Leu Met Pro Ile                                                       
465                                                                       



<210>  4
<211>  469
<212>  PRT
<213>  Influenza A virus


<220>
<221>  MISC_FEATURE
<222>  (1)..(469)
<223>  expressed N2 subtype

<400>  4

Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Ile 
1               5                   10                  15      


Ile Ala Thr Ile Cys Phe Leu Met Gln Ile Ala Ile Leu Val Thr Thr 
            20                  25                  30          


Val Thr Leu His Phe Lys Gln His Asn Cys Asp Ser Ser Pro Asn Asn 
        35                  40                  45              


His Val Met Phe Cys Glu Pro Thr Ile Ile Glu Arg Asn Lys Thr Glu 
    50                  55                  60                  


Ile Val Tyr Leu Thr Asn Thr Thr Val Glu Lys Glu Ile Cys Pro Lys 
65                  70                  75                  80  


Ser Thr Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn Ile Thr Gly 
                85                  90                  95      


Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly 
            100                 105                 110         


Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp His Asp Lys 
        115                 120                 125             


Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn Asn Gly His 
    130                 135                 140                 


Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met 
145                 150                 155                 160 


Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile 
                165                 170                 175     


Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val 
            180                 185                 190         


Cys Ile Thr Gly Asp Asp Glu Asn Ala Thr Ala Ser Phe Ile Tyr Asn 
        195                 200                 205             


Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn Ile Leu Arg 
    210                 215                 220                 


Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val 
225                 230                 235                 240 


Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe 
                245                 250                 255     


Ile Glu Glu Gly Lys Ile Ile His Ile Ser Thr Leu Ser Gly Ser Ala 
            260                 265                 270         


Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg 
        275                 280                 285             


Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp 
    290                 295                 300                 


Ile Asn Val Lys Asp Tyr Ser Thr Val Ser Ser Tyr Val Cys Ser Gly 
305                 310                 315                 320 


Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Phe Ser Ser Ser Asn 
                325                 330                 335     


Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val Lys Gly Trp 
            340                 345                 350         


Ala Phe Asp Asp Gly Asp Asp Leu Trp Met Gly Arg Thr Ile Ser Glu 
        355                 360                 365             


Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Val Lys Gly Trp Ser 
    370                 375                 380                 


Lys Pro Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile Val Asp Arg 
385                 390                 395                 400 


Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Ile Glu Gly Lys Asn 
                405                 410                 415     


Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Lys Glu 
            420                 425                 430         


Glu Thr Lys Val Leu Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 
        435                 440                 445             


Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile 
    450                 455                 460                 


Asn Leu Met Pro Ile 
465                 


<210>  5
<211>  478
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant expressed protein of NA1 containing baculovirus GP67 
       and His tag

<400>  5

Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      


Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          


Ala His Ser Ala Phe Ala His Ser Ile Gln Thr Gly Trp Glu Asn His 
        35                  40                  45              


Thr Gly Met Cys Asn Gln Ser Val Ile Thr Tyr Val Asn Asn Thr Trp 
    50                  55                  60                  


Val Asn Arg Thr Tyr Val Asn Ile Ser Asn Ile Lys Ile Ala Thr Ile 
65                  70                  75                  80  


Gln Asp Val Thr Pro Ile Ile Leu Ala Gly Asn Ser Pro Leu Cys Pro 
                85                  90                  95      


Val Ser Gly Trp Ala Val Tyr Ser Lys Asp Asn Ser Ile Arg Ile Gly 
            100                 105                 110         


Ser Lys Gly Asp Ile Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser 
        115                 120                 125             


Gln Leu Glu Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn 
    130                 135                 140                 


Asp Lys His Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr 
145                 150                 155                 160 


Leu Met Ser Cys Pro Ile Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg 
                165                 170                 175     


Phe Glu Ser Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly 
            180                 185                 190         


Trp Leu Thr Ile Gly Val Ser Gly Pro Asp Asn Gly Ala Val Ala Val 
        195                 200                 205             


Leu Lys Tyr Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Arg Asn 
    210                 215                 220                 


Lys Ile Leu Arg Thr Gln Glu Ser Glu Cys Val Cys Met Asn Gly Ser 
225                 230                 235                 240 


Cys Phe Thr Val Leu Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr 
                245                 250                 255     


Lys Ile Phe Lys Val Glu Lys Gly Lys Ile Ile Lys Ser Ile Glu Leu 
            260                 265                 270         


Asp Ala Pro Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr 
        275                 280                 285             


Gly Lys Val Met Cys Val Cys Arg Asp Asn Trp His Ala Ser Asn Arg 
    290                 295                 300                 


Pro Trp Val Ser Phe Asn Gln Asn Leu Asp Tyr Gln Ile Gly Tyr Ile 
305                 310                 315                 320 


Cys Ser Gly Val Phe Gly Asp Asn Pro Arg Ser Thr Asp Gly Lys Gly 
                325                 330                 335     


Asn Cys Gly Pro Val Leu Ser Asn Gly Ala Asn Gly Val Lys Gly Phe 
            340                 345                 350         


Ser Tyr Arg Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile 
        355                 360                 365             


Asn Ser Arg Ser Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr 
    370                 375                 380                 


Glu Thr Asp Ser Ser Phe Ser Met Lys Gln Asp Ile Ile Ala Leu Asp 
385                 390                 395                 400 


Asp Trp Ser Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr 
                405                 410                 415     


Gly Met Asn Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly 
            420                 425                 430         


Gln Pro Lys Glu Ser Thr Ile Trp Ala Ser Gly Ser Ser Ile Ser Phe 
        435                 440                 445             


Cys Gly Val Asn Ser Glu Thr Ala Asn Trp Ser Trp Pro Asp Gly Ala 
    450                 455                 460                 


Ile Leu Pro Phe Ala Ile Asp Lys His His His His His His 
465                 470                 475             


<210>  6
<211>  478
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant expressed protein of NA2 containing baculovirus GP67 
       and His tag

<400>  6

Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      


Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          


Ala His Ser Ala Phe Ala His Phe Lys Gln His Asn Cys Asp Ser Ser 
        35                  40                  45              


Pro Asn Asn His Val Met Phe Cys Glu Pro Thr Ile Ile Glu Arg Asn 
    50                  55                  60                  


Lys Thr Glu Ile Val Tyr Leu Thr Asn Thr Thr Val Glu Lys Glu Ile 
65                  70                  75                  80  


Cys Pro Lys Ser Thr Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Asn 
                85                  90                  95      


Ile Thr Gly Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser 
            100                 105                 110         


Ala Gly Gly Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp 
        115                 120                 125             


His Asp Lys Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asn 
    130                 135                 140                 


Asn Gly His Ser Asn Asp Thr Val His Asp Arg Thr Pro Tyr Arg Thr 
145                 150                 155                 160 


Leu Leu Met Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln 
                165                 170                 175     


Val Cys Ile Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp 
            180                 185                 190         


Leu His Val Cys Ile Thr Gly Asp Asp Glu Asn Ala Thr Ala Ser Phe 
        195                 200                 205             


Ile Tyr Asn Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn 
    210                 215                 220                 


Ile Leu Arg Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys 
225                 230                 235                 240 


Thr Val Val Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys 
                245                 250                 255     


Ile Leu Phe Ile Glu Glu Gly Lys Ile Ile His Ile Ser Thr Leu Ser 
            260                 265                 270         


Gly Ser Ala Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro 
        275                 280                 285             


Gly Val Arg Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro 
    290                 295                 300                 


Ile Val Asp Ile Asn Val Lys Asp Tyr Ser Thr Val Ser Ser Tyr Val 
305                 310                 315                 320 


Cys Ser Gly Leu Val Gly Asp Thr Pro Arg Lys Asn Asp Ser Phe Ser 
                325                 330                 335     


Ser Ser Asn Cys Leu Asp Pro Asn Asn Glu Glu Gly Gly His Gly Val 
            340                 345                 350         


Lys Gly Trp Ala Phe Asp Asp Gly Asp Asp Leu Trp Met Gly Arg Thr 
        355                 360                 365             


Ile Ser Glu Lys Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Val Lys 
    370                 375                 380                 


Gly Trp Ser Lys Pro Asn Ser Lys Leu Gln Thr Asn Arg Gln Val Ile 
385                 390                 395                 400 


Val Asp Arg Gly Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Ile Glu 
                405                 410                 415     


Gly Lys Asn Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly 
            420                 425                 430         


Arg Lys Glu Glu Thr Lys Val Leu Trp Thr Ser Asn Ser Ile Val Val 
        435                 440                 445             


Phe Cys Gly Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly 
    450                 455                 460                 


Ala Asp Ile Asn Leu Met Pro Ile His His His His His His 
465                 470                 475             


