			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> ENGINEERED TYROSINE AMMONIA LYASE
 
<130> CX7-181WO2
 
<140> 
<141> 2019-12-06
 
<150> US 62/779,647
<151> 2018-12-14
 
<160> 2290
 
<210> 1
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccagcggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cgaaagagct gcagaacaac 300
ctgatgcgtt atcacaaagt gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgact actataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gcgagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 2
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Val Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Tyr Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
 

 
<210> 3
<211> 1683
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Chroogloeocystis siderophila 
 
<400> 3
atgaacaccg ttctgcagac ccaagcgagc accaacctga acgcggacac catcctgctg 60
ggtgatcgta acctgaccat tgacgaggtg gttagcgttg cgcgtcaccg tgcgaaggtg 120
cagatcagca ccgcggataa cgtggagcaa cgtatccagg cgagctgcga ctacattgcg 180
gaagcggttg cgaccggtcg tccgatttat ggcgtgacca gcggtttcgg tggcatggcg 240
aacgtggtta tcagccgtga atacgcggac ctgctgcaac acaacctggt ttggtatcac 300
aaagtgggtg cgggccacaa actgccgctg accgatgttc gtgcggcgat gctgctgcgt 360
gtgaacagcc acctgcacgg tgcgagcggt atccgtcgtg agattgttca gcgtatggaa 420
accttcctga acgcgcgtgt taccccgcac gtgccggagt acggtagcat cggtgcgagc 480
ggtgacctga ccccgctgag ctacatcacc ggcgcgctga ttggtctgga tgaacgttat 540
aaggtggact ttgatggcga ggaaatcgac gcgattaccg cgctggagcg tctgggtctg 600
ccgcagctgc aactgcaggc gaaagaaggc ctggcgatga tgaacggtac cagcgttatg 660
accggcatcg cggcgaactg cgtgtacgat acccgtctgc tgatggcgct gaccatgggt 720
gcgcacgcgc tgattctgca aggcctgaac ggtaccaacc agagcttcca cccgtttatc 780
cacgagctga aaccgcaccc gggtcagaaa tgggcggcga gcaccatgct ggatctgctg 840
gcgggcagcc gtctgattcg tgaggaactg gacggtaccc acgaatatcg tggccaagcg 900
ccgatccagg atcgttacag cctgcgttgc ctggcgcaat atatgggccc gatcgtggac 960
ggtgttgcgc aggtggcgca gcaaattgag gttgaaatga acagcgcgac cgacaacccg 1020
ctgattgatg tggagaacca agcgagctac cacggtggca acttcctggg ccagtatatc 1080
ggtatgggca tggaccacct gcgttactat attggcatga tggcgaagca cctggatgtt 1140
caaatcgcgt acctggtggc gccggagttt aacaacggtc tgccggcgag cctggtgggt 1200
aacaaggaac gtatcgtgaa catgggcctg aaaggtctgc agatcaccgg taacagcatt 1260
atgccgctgc tgagcttcta cggcaacagc attgcggatc gttacccgac ccacgcggag 1320
caatataacc agaacatcaa cagccaaggt tttgcggcgg cgaacctgac ccgtaacgcg 1380
gttgaaatct tccagcaata tatggcgatt gcgctgatgt ttggtgttca ggcggtggat 1440
ctgcgtacct acgcgtatgc gggtcactat gatgcgagcg agtgcctgag cccgaccacc 1500
cgtcgtctgt atcaagcggt gcgtgaagtg gttggtcagc cgccgagcgc ggcgcgtccg 1560
tacatctggg acgatcgtga gcagccgctg gatgaacaca tcgcgaaaat tgcggttgac 1620
atcgcgaccg aaggcgtgat tgttgcggcg gtgaaggacc tgctgaccac cctgaaataa 1680
tga                                                               1683

 
<210> 4
<211> 559
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Chroogloeocystis siderophila 
 
<400> 4
Met Asn Thr Val Leu Gln Thr Gln Ala Ser Thr Asn Leu Asn Ala Asp 
1               5                   10                  15      
Thr Ile Leu Leu Gly Asp Arg Asn Leu Thr Ile Asp Glu Val Val Ser 
            20                  25                  30          
Val Ala Arg His Arg Ala Lys Val Gln Ile Ser Thr Ala Asp Asn Val 
        35                  40                  45              
Glu Gln Arg Ile Gln Ala Ser Cys Asp Tyr Ile Ala Glu Ala Val Ala 
    50                  55                  60                  
Thr Gly Arg Pro Ile Tyr Gly Val Thr Ser Gly Phe Gly Gly Met Ala 
65                  70                  75                  80  
Asn Val Val Ile Ser Arg Glu Tyr Ala Asp Leu Leu Gln His Asn Leu 
                85                  90                  95      
Val Trp Tyr His Lys Val Gly Ala Gly His Lys Leu Pro Leu Thr Asp 
            100                 105                 110         
Val Arg Ala Ala Met Leu Leu Arg Val Asn Ser His Leu His Gly Ala 
        115                 120                 125             
Ser Gly Ile Arg Arg Glu Ile Val Gln Arg Met Glu Thr Phe Leu Asn 
    130                 135                 140                 
Ala Arg Val Thr Pro His Val Pro Glu Tyr Gly Ser Ile Gly Ala Ser 
145                 150                 155                 160 
Gly Asp Leu Thr Pro Leu Ser Tyr Ile Thr Gly Ala Leu Ile Gly Leu 
                165                 170                 175     
Asp Glu Arg Tyr Lys Val Asp Phe Asp Gly Glu Glu Ile Asp Ala Ile 
            180                 185                 190         
Thr Ala Leu Glu Arg Leu Gly Leu Pro Gln Leu Gln Leu Gln Ala Lys 
        195                 200                 205             
Glu Gly Leu Ala Met Met Asn Gly Thr Ser Val Met Thr Gly Ile Ala 
    210                 215                 220                 
Ala Asn Cys Val Tyr Asp Thr Arg Leu Leu Met Ala Leu Thr Met Gly 
225                 230                 235                 240 
Ala His Ala Leu Ile Leu Gln Gly Leu Asn Gly Thr Asn Gln Ser Phe 
                245                 250                 255     
His Pro Phe Ile His Glu Leu Lys Pro His Pro Gly Gln Lys Trp Ala 
            260                 265                 270         
Ala Ser Thr Met Leu Asp Leu Leu Ala Gly Ser Arg Leu Ile Arg Glu 
        275                 280                 285             
Glu Leu Asp Gly Thr His Glu Tyr Arg Gly Gln Ala Pro Ile Gln Asp 
    290                 295                 300                 
Arg Tyr Ser Leu Arg Cys Leu Ala Gln Tyr Met Gly Pro Ile Val Asp 
305                 310                 315                 320 
Gly Val Ala Gln Val Ala Gln Gln Ile Glu Val Glu Met Asn Ser Ala 
                325                 330                 335     
Thr Asp Asn Pro Leu Ile Asp Val Glu Asn Gln Ala Ser Tyr His Gly 
            340                 345                 350         
Gly Asn Phe Leu Gly Gln Tyr Ile Gly Met Gly Met Asp His Leu Arg 
        355                 360                 365             
Tyr Tyr Ile Gly Met Met Ala Lys His Leu Asp Val Gln Ile Ala Tyr 
    370                 375                 380                 
Leu Val Ala Pro Glu Phe Asn Asn Gly Leu Pro Ala Ser Leu Val Gly 
385                 390                 395                 400 
Asn Lys Glu Arg Ile Val Asn Met Gly Leu Lys Gly Leu Gln Ile Thr 
                405                 410                 415     
Gly Asn Ser Ile Met Pro Leu Leu Ser Phe Tyr Gly Asn Ser Ile Ala 
            420                 425                 430         
Asp Arg Tyr Pro Thr His Ala Glu Gln Tyr Asn Gln Asn Ile Asn Ser 
        435                 440                 445             
Gln Gly Phe Ala Ala Ala Asn Leu Thr Arg Asn Ala Val Glu Ile Phe 
    450                 455                 460                 
Gln Gln Tyr Met Ala Ile Ala Leu Met Phe Gly Val Gln Ala Val Asp 
465                 470                 475                 480 
Leu Arg Thr Tyr Ala Tyr Ala Gly His Tyr Asp Ala Ser Glu Cys Leu 
                485                 490                 495     
Ser Pro Thr Thr Arg Arg Leu Tyr Gln Ala Val Arg Glu Val Val Gly 
            500                 505                 510         
Gln Pro Pro Ser Ala Ala Arg Pro Tyr Ile Trp Asp Asp Arg Glu Gln 
        515                 520                 525             
Pro Leu Asp Glu His Ile Ala Lys Ile Ala Val Asp Ile Ala Thr Glu 
    530                 535                 540                 
Gly Val Ile Val Ala Ala Val Lys Asp Leu Leu Thr Thr Leu Lys 
545                 550                 555

<210> 5
<211> 1524
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Flavobacterium johnsoniae 
 
<400> 5
atgaacacca tcaacgagta tctgagcctg gaggaattcg aagcgatcat ttttggtaac 60
caaaaggtga ccattagcga tgtggttgtg aaccgtgtta acgagagctt caactttctg 120
aaggaattca gcggtaacaa agttatctac ggcgtgaaca ccggttttgg cccgatggcg 180
cagtatcgta ttaaagaaag cgaccagatc caactgcagt acaacctgat ccgtagccac 240
agcagcggta ccggtaaacc gctgagcccg gtgtgcgcga aagcggcgat cctggcgcgt 300
ctgaacaccc tgagcctggg taacagcggc gttcacccga gcgtgattaa cctgatgagc 360
gagctgatca acaaggatat taccccgctg atcttcgaac atggtggcgt tggtgcgagc 420
ggtgacctgg tgcaactgag ccacctggcg ctggttctga tcggcgaggg cgaagtgttc 480
tataagggcg agcgtcgtcc gaccccggaa gtgtttgaga ttgaaggcct gaaaccgatt 540
caggttgaga tccgtgaagg tctggcgctg attaacggca ccagcgtgat gaccggtatc 600
ggcgttgtga acgtttatca cgcgaagaaa ctgctggatt ggagcctgaa aagcagctgc 660
gcgatcaacg agctggtgca agcgtacgac gatcacttca gcgcggaact gaaccagacc 720
aagcgtcaca aaggtcaaca agagatcgcg ctgaagatgc gtcagaacct gagcgacagc 780
accctgattc gtaaacgtga ggatcacctg tacagcggtg aaaacaccga ggaaatcttc 840
aaggagaagg ttcaagaata ctatagcctg cgttgcgttc cgcagattct gggcccggtg 900
ctggaaacca ttaacaacgt tgcgagcatc ctggaggacg aattcaacag cgcgaacgac 960
aacccgatca ttgatgttaa gaaccaacac gtgtaccacg gtggcaactt tcacggtgac 1020
tatatcagcc tggagatgga taagctgaaa atcgttatta ccaagctgac catgctggcg 1080
gaacgtcagc tgaactatct gctgaacagc aaaatcaacg agctgctgcc gccgttcgtg 1140
aacctgggta ccctgggctt caactttggt atgcaaggcg ttcagtttac cgcgaccagc 1200
accaccgcgg aaagccaaat gctgagcaac ccgatgtacg tgcacagcat tccgaacaac 1260
aacgacaacc aggatatcgt tagcatgggt accaacagcg cggttattac cagcaaggtg 1320
atcgagaacg cgttcgaagt gctggcgatt gagatgatca ccattgttca agcgatcgac 1380
tatctgggcc agaaggataa aattagcagc gtgagcaaga aatggtacga cgagatccgt 1440
aacatcattc cgaccttcaa agaagatcaa gttatgtacc cgtttgttca gaaggtgaaa 1500
gaccacctga ttaacaacta atga                                        1524

 
<210> 6
<211> 506
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Flavobacterium johnsoniae 
 
<400> 6
Met Asn Thr Ile Asn Glu Tyr Leu Ser Leu Glu Glu Phe Glu Ala Ile 
1               5                   10                  15      
Ile Phe Gly Asn Gln Lys Val Thr Ile Ser Asp Val Val Val Asn Arg 
            20                  25                  30          
Val Asn Glu Ser Phe Asn Phe Leu Lys Glu Phe Ser Gly Asn Lys Val 
        35                  40                  45              
Ile Tyr Gly Val Asn Thr Gly Phe Gly Pro Met Ala Gln Tyr Arg Ile 
    50                  55                  60                  
Lys Glu Ser Asp Gln Ile Gln Leu Gln Tyr Asn Leu Ile Arg Ser His 
65                  70                  75                  80  
Ser Ser Gly Thr Gly Lys Pro Leu Ser Pro Val Cys Ala Lys Ala Ala 
                85                  90                  95      
Ile Leu Ala Arg Leu Asn Thr Leu Ser Leu Gly Asn Ser Gly Val His 
            100                 105                 110         
Pro Ser Val Ile Asn Leu Met Ser Glu Leu Ile Asn Lys Asp Ile Thr 
        115                 120                 125             
Pro Leu Ile Phe Glu His Gly Gly Val Gly Ala Ser Gly Asp Leu Val 
    130                 135                 140                 
Gln Leu Ser His Leu Ala Leu Val Leu Ile Gly Glu Gly Glu Val Phe 
145                 150                 155                 160 
Tyr Lys Gly Glu Arg Arg Pro Thr Pro Glu Val Phe Glu Ile Glu Gly 
                165                 170                 175     
Leu Lys Pro Ile Gln Val Glu Ile Arg Glu Gly Leu Ala Leu Ile Asn 
            180                 185                 190         
Gly Thr Ser Val Met Thr Gly Ile Gly Val Val Asn Val Tyr His Ala 
        195                 200                 205             
Lys Lys Leu Leu Asp Trp Ser Leu Lys Ser Ser Cys Ala Ile Asn Glu 
    210                 215                 220                 
Leu Val Gln Ala Tyr Asp Asp His Phe Ser Ala Glu Leu Asn Gln Thr 
225                 230                 235                 240 
Lys Arg His Lys Gly Gln Gln Glu Ile Ala Leu Lys Met Arg Gln Asn 
                245                 250                 255     
Leu Ser Asp Ser Thr Leu Ile Arg Lys Arg Glu Asp His Leu Tyr Ser 
            260                 265                 270         
Gly Glu Asn Thr Glu Glu Ile Phe Lys Glu Lys Val Gln Glu Tyr Tyr 
        275                 280                 285             
Ser Leu Arg Cys Val Pro Gln Ile Leu Gly Pro Val Leu Glu Thr Ile 
    290                 295                 300                 
Asn Asn Val Ala Ser Ile Leu Glu Asp Glu Phe Asn Ser Ala Asn Asp 
305                 310                 315                 320 
Asn Pro Ile Ile Asp Val Lys Asn Gln His Val Tyr His Gly Gly Asn 
                325                 330                 335     
Phe His Gly Asp Tyr Ile Ser Leu Glu Met Asp Lys Leu Lys Ile Val 
            340                 345                 350         
Ile Thr Lys Leu Thr Met Leu Ala Glu Arg Gln Leu Asn Tyr Leu Leu 
        355                 360                 365             
Asn Ser Lys Ile Asn Glu Leu Leu Pro Pro Phe Val Asn Leu Gly Thr 
    370                 375                 380                 
Leu Gly Phe Asn Phe Gly Met Gln Gly Val Gln Phe Thr Ala Thr Ser 
385                 390                 395                 400 
Thr Thr Ala Glu Ser Gln Met Leu Ser Asn Pro Met Tyr Val His Ser 
                405                 410                 415     
Ile Pro Asn Asn Asn Asp Asn Gln Asp Ile Val Ser Met Gly Thr Asn 
            420                 425                 430         
Ser Ala Val Ile Thr Ser Lys Val Ile Glu Asn Ala Phe Glu Val Leu 
        435                 440                 445             
Ala Ile Glu Met Ile Thr Ile Val Gln Ala Ile Asp Tyr Leu Gly Gln 
    450                 455                 460                 
Lys Asp Lys Ile Ser Ser Val Ser Lys Lys Trp Tyr Asp Glu Ile Arg 
465                 470                 475                 480 
Asn Ile Ile Pro Thr Phe Lys Glu Asp Gln Val Met Tyr Pro Phe Val 
                485                 490                 495     
Gln Lys Val Lys Asp His Leu Ile Asn Asn 
            500                 505

<210> 7
<211> 2085
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Rhodotorula glutinis 
 
<400> 7
atggcgccgc gtccgaccag ccagagccag gcgcgtacct gcccgaccac ccaggtgacc 60
caagttgaca tcgtggagaa gatgctggcg gcgccgaccg acagcaccct ggaactggat 120
ggttacagcc tgaacctggg tgatgtggtt agcgcggcgc gtaagggtcg tccggtgcgt 180
gttaaagaca gcgatgagat ccgtagcaag attgataaaa gcgttgaatt cctgcgtagc 240
cagctgagca tgagcgttta tggtgtgacc accggttttg gtggcagcgc ggacacccgt 300
accgaggatg cgatcagcct gcagaaagcg ctgctggaac accaactgtg cggtgtgctg 360
ccgagcagct tcgatagctt tcgtctgggt cgtggcctgg aaaacagcct gccgctggaa 420
gtggttcgtg gtgcgatgac cattcgtgtt aacagcctga cccgtggtca cagcgcggtg 480
cgtctggtgg ttctggaggc gctgaccaac ttcctgaacc acggcatcac cccgattgtt 540
ccgctgcgtg gtaccattag cgcgagcggt gatctgagcc cgctgagcta cattgcggcg 600
gcgattagcg gtcacccgga tagcaaggtg cacgtggttc acgaaggcaa ggagaaaatt 660
ctgtatgcgc gtgaagcgat ggcgctgttt aacctggagc cggtggttct gggtccgaaa 720
gaaggtctgg gcctggttaa cggtaccgcg gtgagcgcga gcatggcgac cctggcgctg 780
cacgacgcgc acatgctgag cctgctgagc caaagcctga ccgcgatgac cgttgaggcg 840
atggtgggtc acgcgggcag cttccacccg tttctgcacg atgttacccg tccgcacccg 900
acccagattg aggtggcggg taacattcgt aagctgctgg aaggcagccg tttcgcggtt 960
caccacgagg aagaggtgaa ggttaaagac gatgaaggta tcctgcgtca ggaccgttac 1020
ccgctgcgta ccagcccgca atggctgggt ccgctggtta gcgatctgat ccatgcgcat 1080
gcggtgctga ccattgaggc gggtcagagc accaccgaca acccgctgat cgatgttgaa 1140
aacaagacca gccaccacgg tggcaacttc caagcggcgg cggtggcgaa caccatggag 1200
aagacccgtc tgggtctggc gcagattggc aaactgaact ttacccaact gaccgagatg 1260
ctgaacgcgg gtatgaaccg tggcctgccg agctgcctgg cggcggaaga cccgagcctg 1320
agctaccact gcaaaggtct ggatattgct gcggcggcgt ataccagcga gctgggtcac 1380
ctggcgaacc cggttaccac ccatgtgcag ccggcggaaa tggcgaacca agcggttaac 1440
agcctggcgc tgattagcgc gcgtcgtacc accgaaagca acgacgttct gagcctgctg 1500
ctggcgaccc acctgtactg cgtgctgcaa gcgatcgatc tgcgtgcgat tgaattcgag 1560
tttaagaaac agttcggtcc ggcgatcgtg agcctgattg accaacactt tggtagcgcg 1620
atgaccggca gcaacctgcg tgatgaactg gttgagaagg tgaacaaaac cctggcgaag 1680
cgtctggagc agaccaacag ctatgacctg gttccgcgtt ggcacgatgc gttcagcttt 1740
gcggcgggca ccgtggttga agtgctgagc agcaccagcc tgagcctggc ggcggttaac 1800
gcgtggaaag ttgcggcggc ggagagcgcg atcagcctga cccgtcaagt gcgtgaaacc 1860
ttctggagcg cggcgagcac cagcagcccg gcgctgagct acctgagccc gcgtacccaa 1920
attctgtatg cgttcgttcg tgaagagctg ggtgtgaagg cgcgtcgtgg tgacgttttt 1980
ctgggcaaac aggaagtgac catcggtagc aacgtgagca agatctatga agcgattaaa 2040
agcggccgta ttaacaacgt gctgctgaaa atgctggcgt aatga                 2085

 
<210> 8
<211> 693
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Tyrosine Ammonia Lyase from Rhodotorula glutinis 
 
<400> 8
Met Ala Pro Arg Pro Thr Ser Gln Ser Gln Ala Arg Thr Cys Pro Thr 
1               5                   10                  15      
Thr Gln Val Thr Gln Val Asp Ile Val Glu Lys Met Leu Ala Ala Pro 
            20                  25                  30          
Thr Asp Ser Thr Leu Glu Leu Asp Gly Tyr Ser Leu Asn Leu Gly Asp 
        35                  40                  45              
Val Val Ser Ala Ala Arg Lys Gly Arg Pro Val Arg Val Lys Asp Ser 
    50                  55                  60                  
Asp Glu Ile Arg Ser Lys Ile Asp Lys Ser Val Glu Phe Leu Arg Ser 
65                  70                  75                  80  
Gln Leu Ser Met Ser Val Tyr Gly Val Thr Thr Gly Phe Gly Gly Ser 
                85                  90                  95      
Ala Asp Thr Arg Thr Glu Asp Ala Ile Ser Leu Gln Lys Ala Leu Leu 
            100                 105                 110         
Glu His Gln Leu Cys Gly Val Leu Pro Ser Ser Phe Asp Ser Phe Arg 
        115                 120                 125             
Leu Gly Arg Gly Leu Glu Asn Ser Leu Pro Leu Glu Val Val Arg Gly 
    130                 135                 140                 
Ala Met Thr Ile Arg Val Asn Ser Leu Thr Arg Gly His Ser Ala Val 
145                 150                 155                 160 
Arg Leu Val Val Leu Glu Ala Leu Thr Asn Phe Leu Asn His Gly Ile 
                165                 170                 175     
Thr Pro Ile Val Pro Leu Arg Gly Thr Ile Ser Ala Ser Gly Asp Leu 
            180                 185                 190         
Ser Pro Leu Ser Tyr Ile Ala Ala Ala Ile Ser Gly His Pro Asp Ser 
        195                 200                 205             
Lys Val His Val Val His Glu Gly Lys Glu Lys Ile Leu Tyr Ala Arg 
    210                 215                 220                 
Glu Ala Met Ala Leu Phe Asn Leu Glu Pro Val Val Leu Gly Pro Lys 
225                 230                 235                 240 
Glu Gly Leu Gly Leu Val Asn Gly Thr Ala Val Ser Ala Ser Met Ala 
                245                 250                 255     
Thr Leu Ala Leu His Asp Ala His Met Leu Ser Leu Leu Ser Gln Ser 
            260                 265                 270         
Leu Thr Ala Met Thr Val Glu Ala Met Val Gly His Ala Gly Ser Phe 
        275                 280                 285             
His Pro Phe Leu His Asp Val Thr Arg Pro His Pro Thr Gln Ile Glu 
    290                 295                 300                 
Val Ala Gly Asn Ile Arg Lys Leu Leu Glu Gly Ser Arg Phe Ala Val 
305                 310                 315                 320 
His His Glu Glu Glu Val Lys Val Lys Asp Asp Glu Gly Ile Leu Arg 
                325                 330                 335     
Gln Asp Arg Tyr Pro Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Leu 
            340                 345                 350         
Val Ser Asp Leu Ile His Ala His Ala Val Leu Thr Ile Glu Ala Gly 
        355                 360                 365             
Gln Ser Thr Thr Asp Asn Pro Leu Ile Asp Val Glu Asn Lys Thr Ser 
    370                 375                 380                 
His His Gly Gly Asn Phe Gln Ala Ala Ala Val Ala Asn Thr Met Glu 
385                 390                 395                 400 
Lys Thr Arg Leu Gly Leu Ala Gln Ile Gly Lys Leu Asn Phe Thr Gln 
                405                 410                 415     
Leu Thr Glu Met Leu Asn Ala Gly Met Asn Arg Gly Leu Pro Ser Cys 
            420                 425                 430         
Leu Ala Ala Glu Asp Pro Ser Leu Ser Tyr His Cys Lys Gly Leu Asp 
        435                 440                 445             
Ile Ala Ala Ala Ala Tyr Thr Ser Glu Leu Gly His Leu Ala Asn Pro 
    450                 455                 460                 
Val Thr Thr His Val Gln Pro Ala Glu Met Ala Asn Gln Ala Val Asn 
465                 470                 475                 480 
Ser Leu Ala Leu Ile Ser Ala Arg Arg Thr Thr Glu Ser Asn Asp Val 
                485                 490                 495     
Leu Ser Leu Leu Leu Ala Thr His Leu Tyr Cys Val Leu Gln Ala Ile 
            500                 505                 510         
Asp Leu Arg Ala Ile Glu Phe Glu Phe Lys Lys Gln Phe Gly Pro Ala 
        515                 520                 525             
Ile Val Ser Leu Ile Asp Gln His Phe Gly Ser Ala Met Thr Gly Ser 
    530                 535                 540                 
Asn Leu Arg Asp Glu Leu Val Glu Lys Val Asn Lys Thr Leu Ala Lys 
545                 550                 555                 560 
Arg Leu Glu Gln Thr Asn Ser Tyr Asp Leu Val Pro Arg Trp His Asp 
                565                 570                 575     
Ala Phe Ser Phe Ala Ala Gly Thr Val Val Glu Val Leu Ser Ser Thr 
            580                 585                 590         
Ser Leu Ser Leu Ala Ala Val Asn Ala Trp Lys Val Ala Ala Ala Glu 
        595                 600                 605             
Ser Ala Ile Ser Leu Thr Arg Gln Val Arg Glu Thr Phe Trp Ser Ala 
    610                 615                 620                 
Ala Ser Thr Ser Ser Pro Ala Leu Ser Tyr Leu Ser Pro Arg Thr Gln 
625                 630                 635                 640 
Ile Leu Tyr Ala Phe Val Arg Glu Glu Leu Gly Val Lys Ala Arg Arg 
                645                 650                 655     
Gly Asp Val Phe Leu Gly Lys Gln Glu Val Thr Ile Gly Ser Asn Val 
            660                 665                 670         
Ser Lys Ile Tyr Glu Ala Ile Lys Ser Gly Arg Ile Asn Asn Val Leu 
        675                 680                 685             
Leu Lys Met Leu Ala 
    690

<210> 9
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 9
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt tgttatcacc ccggacctgg cgaaggagct gcagcataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 10
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 10
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln His Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 11
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 11
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccacgggt 240
ttcggtggca tggcgaacgt tgttatcacc ccggacctgg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaaag tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgact actataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gcgagcctgg tgggtaacag cgaccgtaaa gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 12
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 12
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ser Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Tyr Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Lys Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 13
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 13
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 14
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 14
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 15
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 15
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccacgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cgaaagagct gcagaacaac 300
ctgatgcgtt atcacaaagt gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgact actataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtaaa gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 16
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 16
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Val Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Tyr Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Lys Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 17
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 17
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaaag tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgact attataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
cctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagt cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 18
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 18
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ser Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Tyr Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Pro Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ser Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 19
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 19
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccacgggt 240
ttcggtggca tggcgaacgt tgttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaaag tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgact attataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 20
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 20
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ser Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Tyr Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 21
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 21
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccacgggt 240
ttcggtggca tggcgaacgt tgttatcacc ccggacctgg cgaaagagct gcagaacaac 300
ctgatgcgtt atcacaaaag tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgact actataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 22
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 22
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ser Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Tyr Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 23
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 23
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccagcggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagt gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gcgagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagt cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 24
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 24
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Val Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ser Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 25
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 25
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cgaaagagct gcagaacaac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagt cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 26
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 26
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ser Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 27
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 27
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt tgttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaaag tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagt cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 28
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 28
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ser Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ser Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 29
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 29
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgacg aagtggcgag tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgccctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 30
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 30
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Ser Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 31
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 31
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 32
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 32
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 33
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 33
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgaca aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 34
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 34
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 35
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 35
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgccctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 36
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 36
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 37
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 37
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgaca aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaagt taatagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 38
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 38
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 39
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 39
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgacg aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 40
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 40
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 41
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 41
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgaca aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgccctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 42
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 42
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 43
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 43
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 44
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 44
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 45
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 45
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggggt taatagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 46
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 46
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 47
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 47
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcggatcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 48
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 48
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Asp Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 49
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 49
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 50
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 50
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 51
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 51
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaagt taatagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 52
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 52
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 53
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 53
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgacg aagtggcgag tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgagtcaa 840
atgctggagc tgctggcggg cagcgccctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 54
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 54
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Ser Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Ser Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 55
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 55
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgacg aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagaaagt taatagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 56
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 56
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 57
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 57
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgcg tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 58
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 58
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Arg Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 59
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 59
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgacg aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgagtcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 60
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 60
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Ser Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 61
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 61
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggggt taatagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgccctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 62
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 62
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 63
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 63
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgag tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaagt taatagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgagtcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 64
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 64
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ser Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Ser Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 65
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 65
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgagtcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 66
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 66
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Ser Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 67
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 67
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 68
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 68
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 69
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 69
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaag aagtggcgca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgccctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 70
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 70
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 71
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 71
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcagt ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 72
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 72
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 73
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 73
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 74
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 74
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 75
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 75
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcact ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aagcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 76
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 76
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 77
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 77
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg ggcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 78
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 78
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Gly 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 79
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 79
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaactc ggaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 80
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 80
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 81
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 81
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 82
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 82
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 83
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 83
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg agcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 84
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 84
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 85
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 85
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaaa ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 86
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 86
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 87
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 87
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg ggcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 88
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 88
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Gly 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 89
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 89
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg ggcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaactc ggaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 90
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 90
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Gly 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 91
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 91
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaaa ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg ggcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 92
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 92
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Gly 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 93
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 93
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 94
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 94
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 95
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 95
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggtcca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 96
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 96
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 97
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 97
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggtcca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg ggcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 98
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 98
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Gly 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 99
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 99
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg agcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaactc ggaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 100
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 100
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 101
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 101
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaaa ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg agcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 102
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 102
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 103
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 103
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaacaa 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 104
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 104
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Gln Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 105
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 105
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggtcca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 106
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 106
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 107
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 107
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggtcca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaaa ggtgaaccga tttacggcgt taccagtggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcagg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
tacatgggcg agcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
atgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 108
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 108
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Met Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Met Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 109
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 109
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggtcca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 110
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 110
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 111
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 111
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggtcca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg ggcagccgat ccaagatcgt tactcgctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 112
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 112
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Gly 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 113
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 113
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 114
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 114
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 115
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 115
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 116
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 116
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 117
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 117
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac caggggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 118
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 118
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Arg Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 119
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 119
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 120
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 120
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 121
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 121
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga tgggccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 122
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 122
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Gly Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 123
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 123
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 124
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 124
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 125
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 125
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattagg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 126
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 126
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 127
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 127
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg gggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 128
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 128
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Gly 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 129
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 129
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatctc gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 130
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 130
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Ser Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 131
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 131
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa cgcggtggtt 1680
agcatt                                                            1686

 
<210> 132
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 132
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Ala Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 133
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 133
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgt ctgatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 134
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 134
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ser 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 135
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 135
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaat atccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 136
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 136
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Tyr Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 137
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 137
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agcaggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 138
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 138
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Gln Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 139
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 139
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatctg tagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 140
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 140
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Cys Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 141
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 141
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
gctatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 142
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 142
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 143
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 143
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa gtggcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 144
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 144
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Val Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 145
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 145
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcggtgaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 146
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 146
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Gly Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 147
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 147
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgtcggc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 148
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 148
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Ser Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 149
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 149
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac ccagggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 150
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 150
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Gln Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 151
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 151
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg tgaggcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 152
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 152
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Glu Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 153
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 153
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggagctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 154
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 154
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Glu Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 155
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 155
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgct ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcagggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 156
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 156
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Arg Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 157
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 157
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcca gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 158
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 158
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 159
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 159
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cacgggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 160
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 160
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Thr Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 161
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 161
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagatt attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 162
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 162
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Ile Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 163
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 163
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg tgttgcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 164
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 164
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 165
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 165
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt gcagatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 166
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 166
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Gln Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 167
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 167
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgttgaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 168
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 168
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Val Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 169
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 169
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
cggctgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 170
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 170
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Arg Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 171
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 171
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta gtgcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 172
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 172
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Ser Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 173
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 173
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taatccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 174
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 174
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Asn Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 175
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 175
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga ttgtccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 176
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 176
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Cys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 177
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 177
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag ctcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 178
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 178
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ser Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 179
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 179
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa cttgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 180
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 180
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Leu Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 181
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 181
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaata aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 182
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 182
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 183
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 183
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagaggctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 184
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 184
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Arg Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 185
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 185
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgag 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 186
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 186
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Glu Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 187
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 187
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac agccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 188
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 188
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 189
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 189
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcgc gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 190
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 190
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Ala Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 191
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 191
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg atgggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 192
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 192
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Met Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 193
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 193
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac caagggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 194
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 194
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 195
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 195
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agctatgaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 196
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 196
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Tyr Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 197
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 197
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggcgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 198
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 198
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Ala Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 199
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 199
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttt atgcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 200
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 200
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Tyr Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 201
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 201
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtgcgg 1680
agcatt                                                            1686

 
<210> 202
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 202
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Arg 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 203
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 203
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaaggct attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 204
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 204
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Ala Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 205
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 205
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 206
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 206
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 207
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 207
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga tgcgccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 208
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 208
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Ala Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 209
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 209
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcaa gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 210
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 210
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Lys Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 211
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 211
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaacagccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 212
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 212
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Gln Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 213
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 213
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgcggct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 214
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 214
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 215
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 215
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaag ctccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 216
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 216
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Ala Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 217
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 217
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccatgtggtt 1680
agcatt                                                            1686

 
<210> 218
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 218
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn His Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 219
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 219
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaaa ctccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 220
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 220
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Thr Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 221
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 221
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcgtgc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 222
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 222
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Arg Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 223
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 223
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggagatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 224
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 224
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Glu Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 225
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 225
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcggc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 226
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 226
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 227
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 227
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cgctggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 228
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 228
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ala Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 229
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 229
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtgact 1680
agcatt                                                            1686

 
<210> 230
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 230
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Thr 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 231
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 231
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacagtggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 232
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 232
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ser 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 233
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 233
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaattgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 234
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 234
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Leu Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 235
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 235
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaaggtg attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 236
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 236
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Val Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 237
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 237
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc gtgcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 238
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 238
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Arg Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 239
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 239
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtataag gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 240
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 240
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Lys Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 241
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 241
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcgg tagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 242
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 242
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Gly Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 243
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 243
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cgggggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 244
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 244
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Gly Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 245
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 245
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaat tgccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 246
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 246
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Leu Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 247
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 247
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ctcggtggtt 1680
agcatt                                                            1686

 
<210> 248
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 248
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Ser Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 249
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 249
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatccg gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 250
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 250
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Arg Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 251
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 251
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcga gcaagtggtt 1680
agcatt                                                            1686

 
<210> 252
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 252
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Glu Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 253
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 253
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 254
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 254
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 255
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 255
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtactccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 256
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 256
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Thr Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 257
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 257
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa cgatgtggtt 1680
agcatt                                                            1686

 
<210> 258
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 258
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Asp Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 259
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 259
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtgggt 1680
agcatt                                                            1686

 
<210> 260
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 260
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Gly 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 261
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 261
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 262
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 262
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 263
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 263
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggggctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 264
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 264
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Gly Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 265
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 265
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgagattc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 266
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 266
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Ile Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 267
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 267
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagagccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 268
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 268
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Glu Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 269
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 269
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgagagtc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 270
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 270
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Ser Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 271
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 271
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttg aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 272
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 272
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Glu Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 273
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 273
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 274
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 274
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 275
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 275
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaaa ttccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 276
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 276
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Ile Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 277
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 277
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacatccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 278
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 278
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
His Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 279
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 279
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtgatg 1680
agcatt                                                            1686

 
<210> 280
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 280
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Met 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 281
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 281
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcggcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 282
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 282
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Arg Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 283
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 283
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagatccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 284
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 284
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Asp Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 285
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 285
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggctc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 286
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 286
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Ala Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 287
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 287
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgagcatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 288
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 288
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu His Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 289
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 289
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg tcgggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 290
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 290
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Ser Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 291
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 291
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga tacgccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 292
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 292
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Thr Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 293
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 293
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg tggggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 294
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 294
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Trp Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 295
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 295
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggtttctagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 296
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 296
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ser Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 297
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 297
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagtcg attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 298
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 298
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Ser Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 299
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 299
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagtggctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 300
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 300
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Trp Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 301
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 301
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 302
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 302
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 303
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 303
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 304
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 304
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 305
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 305
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg gggggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 306
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 306
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Gly Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 307
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 307
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttggg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 308
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 308
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Gly Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 309
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 309
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 310
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 310
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 311
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 311
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc gcgtgcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 312
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 312
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Arg Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 313
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 313
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gatgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 314
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 314
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 315
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 315
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagcacgtgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 316
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 316
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Thr Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 317
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 317
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 318
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 318
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 319
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 319
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcag gcaagtggtt 1680
agcatt                                                            1686

 
<210> 320
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 320
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Arg Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 321
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 321
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aagtggcgca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgagc ggtgaaccga tttacggcgt tacctcgggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcgctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacag cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcga gggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 322
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 322
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Ser Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Ser Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Arg 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 323
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 323
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 324
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 324
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 325
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 325
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 326
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 326
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 327
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 327
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg tgtgccgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcca gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 328
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 328
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 329
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 329
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcca gagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 330
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 330
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 331
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 331
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 332
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 332
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 333
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 333
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 334
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 334
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 335
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 335
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg tgtgccgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcca gagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 336
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 336
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 337
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 337
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 338
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 338
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 339
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 339
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaaa ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 340
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 340
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 341
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 341
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 342
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 342
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 343
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 343
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaggaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 344
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 344
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 345
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 345
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaaga aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaaa ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcca gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 346
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 346
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Lys Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 347
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 347
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaaccgccg 1560
agcgaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 348
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 348
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 349
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 349
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 350
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 350
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 351
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 351
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 352
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 352
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 353
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 353
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taacccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 354
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 354
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Asn Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 355
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 355
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 356
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 356
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 357
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 357
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcgatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 358
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 358
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asp Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 359
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 359
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgagt ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 360
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 360
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Ser Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 361
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 361
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcca gagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 362
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 362
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 363
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 363
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 364
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 364
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 365
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 365
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 366
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 366
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 367
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 367
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 368
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 368
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 369
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 369
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac gacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 370
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 370
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 371
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 371
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagcgcactg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg cggatatcac cagcggtggc cgtattgtta aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 372
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 372
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Ala Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Ala 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Lys Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 373
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 373
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 374
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 374
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 375
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 375
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 376
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 376
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 377
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 377
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtacga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggctcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 378
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 378
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Thr Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 379
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 379
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgcg cgtactgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 380
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 380
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Arg Val Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 381
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 381
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tgaaccaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 382
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 382
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Asn Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 383
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 383
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggctcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagctgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 384
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 384
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 385
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 385
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtacga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggctcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagctgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 386
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 386
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Thr Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 387
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 387
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 388
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 388
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 389
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 389
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagctgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 390
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 390
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 391
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 391
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtacga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagctgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 392
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 392
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Thr Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 393
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 393
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggctcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgtggt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagctgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 394
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 394
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Trp Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 395
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 395
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 396
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 396
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 397
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 397
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac agccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 398
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 398
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 399
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 399
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 400
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 400
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 401
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 401
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac agccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa tcaagtggtt 1680
agcatt                                                            1686

 
<210> 402
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 402
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 403
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 403
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 404
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 404
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 405
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 405
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaacc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac agccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tcaagtggtt 1680
agcatt                                                            1686

 
<210> 406
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 406
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Asn Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 407
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 407
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tcaagtggtt 1680
agcatt                                                            1686

 
<210> 408
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 408
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 409
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 409
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 410
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 410
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 411
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 411
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcataatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 412
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 412
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 413
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 413
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 414
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 414
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 415
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 415
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 416
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 416
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 417
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 417
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tgacgtggtt 1680
agcatt                                                            1686

 
<210> 418
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 418
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Asp Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 419
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 419
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 420
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 420
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 421
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 421
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 422
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 422
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 423
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 423
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac agccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcga ggacgtggtt 1680
agcatt                                                            1686

 
<210> 424
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 424
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Glu Asp Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 425
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 425
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcga gcaagtggtt 1680
agcatt                                                            1686

 
<210> 426
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 426
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Glu Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 427
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 427
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga tgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
ctgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacg 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcga ggacgtggtt 1680
agcatt                                                            1686

 
<210> 428
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 428
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Glu Asp Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 429
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 429
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa tgacgtggtt 1680
agcatt                                                            1686

 
<210> 430
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 430
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Asp Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 431
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 431
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac agccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 432
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 432
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 433
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 433
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taacagcagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt acgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tcaagtggtt 1680
agcatt                                                            1686

 
<210> 434
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 434
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 435
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 435
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg gctgcagatt 1260
tcgggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 436
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 436
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 437
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 437
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaaac tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcgt cgtaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggc gctgcagatt 1260
gcgggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 438
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 438
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Thr Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 439
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 439
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggc gctgcagatt 1260
tcgggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 440
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 440
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 441
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 441
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaaac tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcgt cgtaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 442
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 442
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Thr Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 443
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 443
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgtggt atcacaaagc tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg gctgcagatt 1260
tcgggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 444
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 444
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Trp Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 445
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 445
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctgtcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 446
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 446
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ser Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 447
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 447
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgtggt atcacaaaac tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggc gctgcagatt 1260
tcgggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 448
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 448
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Trp Tyr His Lys Thr Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 449
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 449
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgtggt atcacaaagc tggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtata tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 450
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 450
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Trp Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 451
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 451
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt ttatgaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 452
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 452
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Phe Met Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 453
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 453
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gtaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 454
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 454
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 455
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 455
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 456
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 456
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 457
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 457
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgttacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 458
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 458
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 459
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 459
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 460
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 460
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 461
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 461
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgttgt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 462
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 462
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 463
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 463
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 464
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 464
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 465
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 465
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gttgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 466
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 466
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 467
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 467
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggca atcagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 468
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 468
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Asn 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 469
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 469
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac ggaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 470
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 470
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 471
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 471
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg aagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 472
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 472
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Lys Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 473
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 473
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgagtgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 474
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 474
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Ser Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 475
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 475
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaaagtt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 476
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 476
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Lys Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 477
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 477
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 478
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 478
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 479
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 479
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tgatgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 480
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 480
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Met Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 481
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 481
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 482
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 482
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 483
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 483
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggtggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 484
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 484
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Val Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 485
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 485
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg agtatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 486
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 486
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 487
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 487
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgac tgaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 488
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 488
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Thr Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 489
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 489
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacaa ggaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 490
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 490
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Lys Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 491
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 491
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcttgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 492
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 492
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Leu Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 493
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 493
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctgcgtgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccctgca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 494
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 494
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Arg Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Leu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 495
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 495
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggacctgg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatctggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgaccgttca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 496
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 496
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Leu Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Val Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 497
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 497
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaatgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 498
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 498
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 499
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 499
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 500
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 500
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 501
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 501
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggcgaatgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 502
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 502
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 503
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 503
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 504
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 504
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 505
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 505
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgaatgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 506
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 506
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 507
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 507
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgaatgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 508
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 508
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 509
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 509
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 510
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 510
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 511
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 511
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 512
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 512
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 513
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 513
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 514
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 514
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 515
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 515
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 516
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 516
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 517
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 517
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 518
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 518
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 519
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 519
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 520
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 520
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 521
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 521
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 522
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 522
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 523
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 523
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac aaccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 524
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 524
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Gln Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 525
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 525
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 526
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 526
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 527
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 527
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaaacc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 528
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 528
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Thr Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 529
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 529
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 530
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 530
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 531
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 531
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccga cgttgcgcgt 120
tatggtgcga aggcgctgct gaccaataaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
actggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 532
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 532
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Asp Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asn Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 533
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 533
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
agtggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 534
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 534
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 535
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 535
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 536
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 536
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 537
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 537
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 538
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 538
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 539
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 539
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 540
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 540
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 541
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 541
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 542
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 542
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 543
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 543
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 544
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 544
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 545
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 545
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 546
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 546
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 547
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 547
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 548
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 548
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 549
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 549
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 550
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 550
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 551
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 551
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 552
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 552
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 553
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 553
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 554
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 554
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 555
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 555
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 556
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 556
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 557
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 557
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 558
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 558
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 559
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 559
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgagtgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 560
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 560
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Ser Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 561
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 561
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgagtgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 562
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 562
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Ser Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 563
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 563
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 564
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 564
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 565
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 565
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcca aagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 566
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 566
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 567
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 567
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgagtgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 568
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 568
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Ser Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 569
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 569
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 570
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 570
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 571
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 571
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcca aagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 572
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 572
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 573
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 573
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcact gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ttgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 574
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 574
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 575
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 575
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 576
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 576
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 577
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 577
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg accatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 578
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 578
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 579
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 579
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 580
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 580
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 581
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 581
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 582
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 582
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 583
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 583
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 584
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 584
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 585
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 585
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 586
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 586
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 587
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 587
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 588
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 588
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 589
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 589
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacgtt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgaa gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcca aagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 590
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 590
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Lys Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gln Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 591
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 591
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggcggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 592
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 592
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 593
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 593
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 594
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 594
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 595
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 595
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg cgacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 596
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 596
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 597
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 597
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 598
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 598
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 599
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 599
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacgtt cctgaacgcg ggcgtgaccc cgagtgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 600
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 600
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Ser Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 601
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 601
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 602
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 602
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 603
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 603
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 604
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 604
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 605
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 605
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccag cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 606
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 606
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Ser Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 607
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 607
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg tcgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 608
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 608
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Ser Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 609
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 609
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cggcggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacgtt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 610
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 610
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Ala Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 611
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 611
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacgtt cctgaacgcg ggcgtgaccc cgcatgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacgt cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcca aagcggcggc cgtattgttc aggcgatcaa ccaggtggtt 1680
agcatt                                                            1686

 
<210> 612
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 612
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Val Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gln Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 613
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 613
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
gtcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgagtgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctggccgg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tagcgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ccacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 614
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 614
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Val Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Ser Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Ala Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 615
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 615
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 616
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 616
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 617
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 617
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 618
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 618
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 619
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 619
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg atgggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 620
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 620
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Met Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 621
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 621
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 622
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 622
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 623
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 623
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg atgggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 624
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 624
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Met Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 625
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 625
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 626
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 626
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 627
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 627
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 628
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 628
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 629
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 629
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 630
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 630
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 631
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 631
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 632
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 632
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 633
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 633
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 634
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 634
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 635
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 635
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 636
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 636
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 637
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 637
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 638
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 638
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 639
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 639
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 640
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 640
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 641
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 641
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 642
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 642
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 643
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 643
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 644
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 644
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 645
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 645
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 646
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 646
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 647
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 647
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg atgggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 648
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 648
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Met Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 649
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 649
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 650
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 650
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 651
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 651
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 652
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 652
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 653
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 653
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac tgccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 654
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 654
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Leu Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 655
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 655
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 656
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 656
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 657
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 657
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac tgccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 658
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 658
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Leu Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 659
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 659
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 660
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 660
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 661
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 661
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 662
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 662
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 663
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 663
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 664
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 664
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 665
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 665
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 666
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 666
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 667
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 667
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg atgggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 668
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 668
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu Met Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 669
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 669
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggtcgc ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg catggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ttcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tattatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gcccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 670
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 670
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Val Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ser Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 671
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 671
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgttgaaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaggatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 672
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 672
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Glu Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 673
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 673
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagaaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 674
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 674
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Glu Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 675
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 675
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgcct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgctggtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 676
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 676
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Leu Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 677
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 677
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga cgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgttgcaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagt cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaggatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 678
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 678
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ser Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 679
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 679
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 680
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 680
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 681
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 681
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 682
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 682
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 683
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 683
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 684
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 684
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 685
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 685
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaggatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 686
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 686
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 687
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 687
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 688
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 688
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 689
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 689
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 690
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 690
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 691
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 691
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 692
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 692
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 693
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 693
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagaaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaggatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 694
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 694
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Glu Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 695
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 695
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 696
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 696
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 697
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 697
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 698
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 698
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 699
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 699
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgcct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga cgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 700
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 700
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 701
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 701
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgcct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 702
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 702
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 703
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 703
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 704
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 704
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 705
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 705
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgcct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgttgaaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaggatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 706
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 706
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Glu Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 707
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 707
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgcct gaccgataaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 708
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 708
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 709
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 709
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaaccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgacgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagaaccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 710
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 710
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Thr Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Glu Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 711
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 711
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccactg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
gcaatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 712
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 712
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 713
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 713
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 714
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 714
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 715
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 715
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcgtgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 716
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 716
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Arg Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 717
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 717
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccactg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagacc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 718
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 718
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 719
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 719
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggagctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tcaagtggtg 1680
agcatt                                                            1686

 
<210> 720
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 720
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Glu Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 721
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 721
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tcaagtggtg 1680
agcatt                                                            1686

 
<210> 722
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 722
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 723
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 723
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagccc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcga acaagtggtg 1680
agcatt                                                            1686

 
<210> 724
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 724
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Ala Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Glu Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 725
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 725
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggagctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 726
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 726
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Glu Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 727
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 727
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggggctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 728
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 728
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Gly Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 729
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 729
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taaggagctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagacc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 730
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 730
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Glu Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 731
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 731
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctga gcctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgct ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtataag gcgattaagg aagtggttgg taaagcgccg 1560
gataaagcac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
gcaatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa tgacgtggtg 1680
agcatt                                                            1686

 
<210> 732
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 732
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Lys Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Ala Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Asp Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 733
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 733
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 734
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 734
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 735
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 735
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 736
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 736
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 737
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 737
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggaac tgctgcaggg cagccgcctg gttcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 738
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 738
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 739
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 739
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 740
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 740
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 741
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 741
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctgcctcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 742
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 742
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Pro Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 743
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 743
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 744
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 744
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 745
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 745
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 746
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 746
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 747
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 747
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggaac tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 748
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 748
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 749
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 749
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 750
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 750
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 751
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 751
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 752
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 752
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 753
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 753
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctgtcgc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 754
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 754
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Ser Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 755
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 755
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 756
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 756
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 757
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 757
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggaac tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 758
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 758
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 759
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 759
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggaac tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 760
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 760
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 761
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 761
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccaactg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 762
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 762
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 763
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 763
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 764
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 764
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 765
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 765
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc ttgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 766
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 766
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Leu Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 767
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 767
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 768
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 768
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 769
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 769
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccaactg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 770
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 770
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 771
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 771
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggaac tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 772
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 772
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 773
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 773
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 774
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 774
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 775
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 775
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaggt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 776
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 776
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 777
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 777
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggaac tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 778
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 778
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 779
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 779
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt taaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 780
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 780
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 781
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 781
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctgcctcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctgtcgc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 782
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 782
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Pro Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Ser Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 783
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 783
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg attcgtgacg agctggatgg tagccacgac 900
taccgtggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 784
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 784
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 785
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 785
atgatcattg cgagccagac ccaaaccttc accagcaaat ttccgcacac cgcgagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagaaagt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 786
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 786
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Phe Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Lys Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 787
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 787
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 788
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 788
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 789
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 789
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 790
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 790
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 791
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 791
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 792
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 792
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 793
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 793
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 794
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 794
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 795
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 795
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 796
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 796
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 797
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 797
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 798
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 798
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 799
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 799
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 800
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 800
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 801
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 801
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 802
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 802
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 803
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 803
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 804
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 804
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 805
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 805
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 806
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 806
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 807
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 807
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 808
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 808
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 809
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 809
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 810
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 810
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 811
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 811
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 812
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 812
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 813
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 813
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 814
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 814
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 815
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 815
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 816
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 816
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 817
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 817
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 818
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 818
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 819
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 819
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 820
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 820
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 821
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 821
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 822
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 822
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 823
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 823
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 824
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 824
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 825
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 825
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 826
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 826
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 827
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 827
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 828
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 828
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 829
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 829
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 830
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 830
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 831
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 831
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 832
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 832
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 833
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 833
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 834
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 834
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 835
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 835
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 836
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 836
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 837
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 837
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 838
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 838
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 839
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 839
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 840
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 840
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 841
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 841
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 842
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 842
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 843
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 843
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggatc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 844
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 844
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Asp Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 845
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 845
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 846
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 846
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 847
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 847
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 848
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 848
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 849
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 849
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 850
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 850
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 851
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 851
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 852
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 852
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 853
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 853
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 854
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 854
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 855
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 855
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 856
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 856
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 857
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 857
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 858
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 858
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 859
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 859
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaac cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 860
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 860
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Thr Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 861
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 861
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 862
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 862
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 863
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 863
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 864
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 864
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 865
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 865
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 866
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 866
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 867
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 867
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 868
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 868
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 869
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 869
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgtgacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 870
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 870
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Val Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 871
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 871
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgagacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tcaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 872
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 872
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Gln Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 873
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 873
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg gtccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 874
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 874
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 875
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 875
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 876
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 876
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 877
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 877
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 878
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 878
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 879
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 879
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 880
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 880
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 881
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 881
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 882
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 882
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 883
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 883
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 884
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 884
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 885
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 885
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 886
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 886
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 887
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 887
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 888
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 888
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 889
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 889
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 890
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 890
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 891
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 891
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 892
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 892
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 893
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 893
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 894
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 894
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 895
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 895
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 896
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 896
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 897
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 897
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 898
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 898
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 899
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 899
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 900
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 900
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 901
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 901
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 902
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 902
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 903
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 903
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 904
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 904
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 905
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 905
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 906
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 906
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 907
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 907
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 908
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 908
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 909
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 909
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 910
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 910
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 911
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 911
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 912
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 912
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 913
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 913
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcgaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 914
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 914
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Glu Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 915
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 915
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 916
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 916
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 917
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 917
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 918
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 918
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 919
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 919
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 920
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 920
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 921
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 921
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 922
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 922
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 923
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 923
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 924
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 924
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 925
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 925
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 926
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 926
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 927
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 927
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 928
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 928
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 929
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 929
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 930
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 930
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 931
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 931
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 932
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 932
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 933
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 933
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 934
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 934
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 935
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 935
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 936
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 936
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 937
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 937
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 938
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 938
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 939
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 939
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 940
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 940
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 941
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 941
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 942
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 942
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 943
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 943
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 944
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 944
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 945
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 945
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 946
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 946
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 947
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 947
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 948
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 948
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 949
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 949
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 950
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 950
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 951
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 951
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 952
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 952
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 953
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 953
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 954
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 954
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 955
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 955
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 956
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 956
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 957
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 957
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 958
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 958
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 959
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 959
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 960
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 960
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 961
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 961
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 962
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 962
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 963
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 963
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 964
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 964
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 965
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 965
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 966
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 966
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 967
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 967
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 968
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 968
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 969
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 969
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 970
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 970
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 971
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 971
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 972
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 972
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 973
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 973
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 974
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 974
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 975
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 975
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 976
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 976
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 977
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 977
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 978
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 978
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 979
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 979
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 980
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 980
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 981
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 981
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 982
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 982
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 983
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 983
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 984
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 984
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 985
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 985
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 986
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 986
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 987
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 987
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 988
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 988
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 989
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 989
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 990
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 990
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 991
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 991
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctactgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 992
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 992
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 993
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 993
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 994
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 994
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 995
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 995
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 996
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 996
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 997
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 997
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgcag 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 998
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 998
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Gln Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 999
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 999
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1000
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1000
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1001
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1001
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1002
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1002
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1003
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1003
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1004
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1004
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1005
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1005
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1006
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1006
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1007
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1007
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1008
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1008
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1009
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1009
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1010
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1010
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1011
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1011
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1012
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1012
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1013
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1013
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1014
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1014
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1015
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1015
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1016
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1016
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1017
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1017
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1018
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1018
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1019
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1019
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1020
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1020
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1021
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1021
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1022
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1022
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1023
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1023
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1024
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1024
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1025
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1025
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1026
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1026
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1027
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1027
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1028
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1028
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1029
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1029
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1030
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1030
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1031
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1031
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1032
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1032
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1033
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1033
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1034
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1034
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1035
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1035
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1036
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1036
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1037
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1037
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1038
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1038
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1039
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1039
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1040
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1040
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1041
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1041
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1042
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1042
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1043
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1043
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1044
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1044
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1045
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1045
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1046
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1046
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1047
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1047
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1048
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1048
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1049
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1049
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1050
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1050
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1051
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1051
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1052
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1052
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1053
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1053
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1054
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1054
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1055
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1055
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaagaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1056
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1056
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1057
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1057
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1058
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1058
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1059
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1059
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1060
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1060
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1061
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1061
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1062
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1062
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1063
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1063
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1064
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1064
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1065
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1065
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1066
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1066
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1067
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1067
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1068
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1068
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1069
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1069
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1070
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1070
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1071
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1071
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1072
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1072
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1073
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1073
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1074
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1074
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1075
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1075
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1076
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1076
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1077
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1077
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1078
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1078
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1079
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1079
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1080
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1080
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1081
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1081
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1082
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1082
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1083
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1083
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1084
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1084
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1085
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1085
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1086
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1086
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1087
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1087
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1088
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1088
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1089
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1089
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1090
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1090
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1091
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1091
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1092
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1092
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1093
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1093
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgaaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1094
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1094
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Lys Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1095
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1095
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1096
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1096
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1097
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1097
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1098
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1098
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1099
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1099
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1100
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1100
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1101
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1101
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1102
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1102
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1103
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1103
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1104
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1104
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1105
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1105
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1106
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1106
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1107
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1107
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1108
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1108
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1109
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1109
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1110
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1110
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1111
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1111
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1112
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1112
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1113
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1113
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1114
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1114
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1115
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1115
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1116
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1116
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1117
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1117
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1118
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1118
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1119
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1119
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1120
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1120
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1121
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1121
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1122
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1122
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1123
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1123
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1124
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1124
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1125
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1125
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1126
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1126
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1127
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1127
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1128
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1128
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1129
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1129
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1130
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1130
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1131
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1131
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1132
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1132
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1133
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1133
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1134
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1134
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1135
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1135
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1136
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1136
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1137
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1137
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1138
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1138
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1139
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1139
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtacctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1140
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1140
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Pro Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1141
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1141
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1142
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1142
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1143
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1143
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1144
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1144
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1145
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1145
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1146
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1146
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1147
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1147
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1148
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1148
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1149
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1149
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1150
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1150
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1151
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1151
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1152
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1152
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1153
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1153
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1154
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1154
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1155
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1155
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1156
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1156
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1157
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1157
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg tgtagctccg 1560
gataaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1158
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1158
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Val Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1159
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1159
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1160
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1160
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1161
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1161
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1162
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1162
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1163
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1163
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1164
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1164
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1165
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1165
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1166
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1166
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1167
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1167
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1168
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1168
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1169
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1169
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
gataaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1170
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1170
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1171
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1171
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgccgaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag cagtatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
tcgaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
gccatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1172
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1172
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Ala Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1173
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1173
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaacg aggtggccca agttgcgcgt 120
tatggtgcga aggcgcgtct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggttga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaacctccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caagggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1174
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1174
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1175
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1175
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc cgttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac ctcgggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1176
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1176
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1177
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1177
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1178
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1178
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1179
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1179
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1180
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1180
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1181
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1181
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1182
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1182
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1183
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1183
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1184
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1184
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1185
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1185
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1186
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1186
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1187
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1187
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1188
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1188
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1189
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1189
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcgtgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1190
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1190
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Val Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1191
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1191
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1192
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1192
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1193
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1193
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1194
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1194
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1195
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1195
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1196
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1196
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1197
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1197
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1198
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1198
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1199
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1199
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1200
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1200
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1201
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1201
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1202
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1202
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1203
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1203
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcagcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1204
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1204
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Ser Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1205
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1205
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1206
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1206
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1207
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1207
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1208
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1208
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1209
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1209
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1210
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1210
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1211
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1211
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1212
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1212
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1213
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1213
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1214
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1214
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1215
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1215
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1216
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1216
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1217
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1217
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1218
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1218
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1219
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1219
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1220
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1220
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1221
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1221
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1222
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1222
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1223
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1223
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1224
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1224
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1225
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1225
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1226
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1226
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1227
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1227
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1228
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1228
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1229
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1229
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1230
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1230
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1231
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1231
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1232
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1232
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1233
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1233
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1234
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1234
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1235
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1235
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1236
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1236
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1237
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1237
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1238
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1238
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1239
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1239
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1240
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1240
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1241
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1241
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1242
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1242
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1243
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1243
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1244
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1244
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1245
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1245
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1246
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1246
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1247
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1247
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1248
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1248
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1249
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1249
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1250
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1250
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1251
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1251
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1252
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1252
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1253
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1253
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1254
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1254
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1255
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1255
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1256
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1256
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1257
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1257
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1258
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1258
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1259
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1259
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1260
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1260
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1261
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1261
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1262
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1262
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1263
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1263
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1264
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1264
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1265
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1265
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1266
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1266
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1267
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1267
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1268
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1268
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1269
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1269
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1270
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1270
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1271
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1271
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1272
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1272
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1273
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1273
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1274
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1274
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1275
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1275
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1276
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1276
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1277
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1277
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1278
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1278
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1279
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1279
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1280
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1280
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1281
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1281
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1282
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1282
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1283
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1283
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1284
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1284
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1285
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1285
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1286
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1286
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1287
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1287
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1288
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1288
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1289
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1289
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1290
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1290
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1291
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1291
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1292
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1292
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1293
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1293
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1294
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1294
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1295
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1295
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1296
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1296
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1297
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1297
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1298
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1298
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1299
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1299
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1300
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1300
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1301
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1301
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1302
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1302
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1303
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1303
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1304
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1304
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1305
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1305
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1306
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1306
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1307
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1307
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1308
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1308
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1309
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1309
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1310
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1310
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1311
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1311
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1312
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1312
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1313
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1313
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1314
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1314
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1315
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1315
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1316
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1316
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1317
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1317
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1318
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1318
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1319
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1319
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1320
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1320
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1321
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1321
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1322
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1322
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1323
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1323
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1324
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1324
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1325
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1325
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1326
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1326
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1327
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1327
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1328
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1328
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1329
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1329
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1330
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1330
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1331
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1331
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1332
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1332
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1333
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1333
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1334
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1334
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1335
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1335
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1336
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1336
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1337
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1337
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1338
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1338
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1339
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1339
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1340
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1340
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1341
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1341
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1342
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1342
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1343
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1343
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1344
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1344
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1345
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1345
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1346
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1346
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1347
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1347
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1348
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1348
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1349
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1349
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1350
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1350
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1351
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1351
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1352
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1352
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1353
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1353
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1354
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1354
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1355
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1355
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1356
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1356
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1357
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1357
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1358
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1358
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1359
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1359
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgcctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1360
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1360
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1361
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1361
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1362
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1362
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1363
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1363
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagc                                                            1686

 
<210> 1364
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1364
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1365
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1365
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1366
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1366
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1367
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1367
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1368
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1368
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1369
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1369
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1370
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1370
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1371
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1371
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1372
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1372
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1373
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1373
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
tcgaaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatc                                                            1686

 
<210> 1374
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1374
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1375
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1375
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1376
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1376
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1377
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1377
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagagc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1378
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1378
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1379
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1379
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac gatgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagcgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1380
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1380
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asp Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1381
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1381
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccaccaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccaactg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1382
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1382
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

 
<210> 1383
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1383
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccactaaa gaggaagtgc tgcagcgcgt taacagcagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccgtctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaagcgccg 1560
gataaagatc gtccgtatat ctggaacgac aatgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcatt                                                            1686

 
<210> 1384
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1384
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Thr Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Arg Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Asp Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ile                                                         
                                                                

<210> 1385
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1385
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aagcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aagcgtctgg gtctggagcc gctggagctg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgcaacaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaacgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gcagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1386
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1386
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Gln Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Glu Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1387
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1387
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcgc gcgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca gctgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1388
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1388
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1389
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1389
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aagcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaacgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gcagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagtcaatgt 1500
ctgagcccgc gcacccgtca gctgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1390
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1390
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Glu Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1391
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1391
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aagcgtctgg gtctggagcc gctggagctg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaaacaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg aacaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagtcaatgt 1500
ctgagcccgc gcacccgtca gctgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1392
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1392
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Asn Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1393
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1393
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcgc gcgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aagctgctgg gtctggagcc gctggagctg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagtcaatgt 1500
ctgagcccgc gcacccgtca gctgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1394
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1394
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1395
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1395
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aatcgtctgg gtctggagcc gctggagctg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg aacaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gcagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagtcaatgt 1500
ctgagcccgc gcacccgtcg tctgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1396
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1396
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Asn Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Asn Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Arg Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1397
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1397
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aagcgtctgg gtctggagcc gctggagctg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1398
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1398
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1399
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1399
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagtcagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1400
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1400
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1401
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1401
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1402
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1402
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1403
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1403
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagtgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1404
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1404
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1405
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1405
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1406
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1406
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1407
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1407
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1408
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1408
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1409
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1409
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1410
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1410
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1411
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1411
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgccctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1412
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1412
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1413
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1413
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1414
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1414
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1415
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1415
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1416
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1416
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1417
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1417
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagtggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1418
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1418
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1419
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1419
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgtgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1420
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1420
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Val Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1421
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1421
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1422
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1422
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1423
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1423
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1424
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1424
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1425
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1425
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1426
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1426
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1427
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1427
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1428
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1428
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1429
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1429
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1430
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1430
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1431
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1431
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1432
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1432
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1433
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1433
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1434
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1434
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1435
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1435
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1436
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1436
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1437
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1437
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1438
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1438
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1439
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1439
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1440
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1440
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1441
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1441
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1442
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1442
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1443
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1443
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1444
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1444
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1445
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1445
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1446
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1446
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1447
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1447
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1448
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1448
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1449
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1449
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1450
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1450
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1451
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1451
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1452
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1452
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1453
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1453
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1454
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1454
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1455
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1455
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcca gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1456
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1456
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1457
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1457
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1458
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1458
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1459
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1459
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1460
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1460
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1461
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1461
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1462
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1462
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1463
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1463
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1464
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1464
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1465
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1465
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagtggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1466
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1466
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1467
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1467
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1468
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1468
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1469
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1469
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1470
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1470
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1471
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1471
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1472
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1472
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1473
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1473
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagtggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1474
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1474
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1475
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1475
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1476
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1476
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1477
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1477
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1478
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1478
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1479
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1479
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1480
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1480
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1481
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1481
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1482
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1482
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1483
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1483
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagtggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1484
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1484
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1485
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1485
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1486
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1486
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1487
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1487
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1488
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1488
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1489
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1489
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1490
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1490
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1491
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1491
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1492
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1492
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1493
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1493
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1494
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1494
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1495
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1495
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagtgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1496
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1496
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1497
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1497
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1498
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1498
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1499
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1499
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1500
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1500
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1501
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1501
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1502
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1502
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1503
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1503
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tcaggaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac cagtgacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1504
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1504
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Gln Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1505
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1505
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1506
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1506
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1507
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1507
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaagacggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1508
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1508
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1509
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1509
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagagc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1510
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1510
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1511
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1511
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgagatcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1512
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1512
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1513
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1513
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1514
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1514
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1515
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1515
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagggcgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1516
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1516
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1517
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1517
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaacg ctatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1518
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1518
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ala Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1519
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1519
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgcctcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1520
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1520
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1521
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1521
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc gctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1522
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1522
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1523
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1523
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tgtgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1524
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1524
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Val Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1525
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1525
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagctg 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1526
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1526
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Leu Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1527
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1527
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctgcgctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1528
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1528
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ala Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1529
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1529
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aatatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1530
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1530
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1531
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1531
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accgctccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1532
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1532
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Ala Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1533
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1533
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1534
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1534
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1535
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1535
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaaacg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1536
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1536
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Thr Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1537
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1537
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgtt aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1538
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1538
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Val Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1539
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1539
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcattc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1540
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1540
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Ile Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1541
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1541
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccgg cttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1542
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1542
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ala Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1543
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1543
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gctttaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1544
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1544
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Phe Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1545
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1545
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaagg aggtggccca ggttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc ggtgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1546
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1546
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Lys Glu Val Ala Gln Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1547
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1547
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1548
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1548
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1549
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1549
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1550
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1550
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1551
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1551
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1552
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1552
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1553
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1553
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1554
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1554
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1555
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1555
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1556
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1556
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1557
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1557
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1558
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1558
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1559
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1559
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1560
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1560
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1561
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1561
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1562
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1562
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1563
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1563
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1564
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1564
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1565
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1565
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1566
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1566
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1567
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1567
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1568
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1568
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1569
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1569
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1570
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1570
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1571
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1571
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1572
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1572
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1573
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1573
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1574
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1574
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1575
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1575
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1576
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1576
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1577
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1577
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1578
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1578
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1579
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1579
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1580
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1580
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1581
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1581
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1582
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1582
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1583
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1583
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1584
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1584
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1585
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1585
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1586
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1586
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1587
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1587
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1588
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1588
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1589
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1589
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1590
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1590
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1591
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1591
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1592
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1592
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1593
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1593
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1594
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1594
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1595
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1595
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1596
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1596
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1597
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1597
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1598
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1598
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1599
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1599
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1600
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1600
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1601
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1601
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1602
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1602
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1603
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1603
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1604
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1604
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1605
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1605
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1606
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1606
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1607
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1607
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1608
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1608
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1609
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1609
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgctgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1610
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1610
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1611
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1611
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1612
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1612
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1613
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1613
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1614
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1614
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1615
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1615
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg aacatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1616
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1616
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Asn Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1617
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1617
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1618
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1618
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1619
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1619
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1620
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1620
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1621
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1621
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1622
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1622
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1623
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1623
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1624
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1624
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1625
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1625
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1626
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1626
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1627
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1627
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1628
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1628
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1629
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1629
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1630
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1630
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1631
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1631
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgctgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgatgcgtt atcacaaagc gggtgcgggc aagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgt 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1632
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1632
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Leu Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Met Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1633
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1633
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ttgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gtgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggc cctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1634
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1634
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ile Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Ala Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser        


<210> 1635
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1635
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gcgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgct 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1636
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1636
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1637
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1637
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccactgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcgc 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1638
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1638
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1639
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1639
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1640
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1640
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1641
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1641
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccactgt atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1642
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1642
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1643
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1643
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1644
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1644
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1645
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1645
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gcgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1646
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1646
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1647
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1647
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1648
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1648
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1649
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1649
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1650
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1650
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1651
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1651
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1652
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1652
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1653
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1653
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccactgt atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1654
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1654
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Leu Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1655
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1655
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1656
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1656
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1657
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1657
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgct 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1658
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1658
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1659
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1659
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1660
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1660
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1661
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1661
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccactgt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1662
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1662
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1663
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1663
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1664
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1664
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1665
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1665
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1666
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1666
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1667
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1667
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccactgt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1668
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1668
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1669
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1669
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1670
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1670
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1671
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1671
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1672
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1672
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1673
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1673
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgct 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1674
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1674
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1675
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1675
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagggtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1676
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1676
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Gly Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1677
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1677
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gcgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1678
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1678
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1679
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1679
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccactgt atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1680
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1680
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Leu Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1681
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1681
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1682
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1682
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1683
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1683
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aagcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgccagcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1684
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1684
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Gln Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1685
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1685
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggttt cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1686
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1686
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Ser Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1687
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1687
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg ctacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1688
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1688
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1689
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1689
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
ctgggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1690
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1690
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Leu Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1691
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1691
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtt cgagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1692
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1692
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Ser 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1693
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1693
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattagg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1694
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1694
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Arg Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1695
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1695
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
aatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1696
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1696
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Asn Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1697
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1697
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgta atagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1698
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1698
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Asn 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1699
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1699
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc ttacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1700
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1700
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Leu Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1701
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1701
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
catggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1702
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1702
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg His Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1703
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1703
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1704
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1704
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Lys Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1705
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1705
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa catgaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1706
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1706
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys His Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1707
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1707
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagagccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1708
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1708
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1709
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1709
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgtctct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1710
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1710
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1711
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1711
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacagtagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1712
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1712
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1713
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1713
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgg aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1714
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1714
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Glu Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1715
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1715
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgactgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1716
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1716
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Thr Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1717
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1717
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1718
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1718
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1719
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1719
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1720
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1720
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1721
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1721
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
agtggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1722
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1722
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Ser Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1723
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1723
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc gggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1724
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1724
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1725
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1725
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
cagggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1726
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1726
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1727
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1727
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct ggcggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1728
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1728
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Ala 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1729
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1729
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaacatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1730
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1730
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys His Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1731
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1731
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaatgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1732
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1732
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1733
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1733
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtctgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1734
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1734
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1735
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1735
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacaatagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1736
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1736
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Asn Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1737
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1737
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacatg gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1738
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1738
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Met Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1739
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1739
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgt cggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1740
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1740
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Ser Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1741
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1741
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taagccgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1742
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1742
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Pro Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1743
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1743
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggagccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1744
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1744
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Glu Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1745
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1745
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggttc caagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1746
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1746
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Ser Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1747
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1747
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgacggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1748
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1748
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Thr Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1749
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1749
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1750
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1750
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1751
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1751
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcgc ctgcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1752
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1752
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Pro Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1753
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1753
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggttg aggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1754
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1754
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Glu Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1755
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1755
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg ttcggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1756
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1756
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1757
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1757
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1758
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1758
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1759
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1759
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gggtgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1760
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1760
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Gly 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1761
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1761
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattcat 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1762
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1762
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile His Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1763
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1763
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1764
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1764
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1765
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1765
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcatgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat acggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1766
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1766
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala His Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Arg 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1767
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1767
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg gaaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1768
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1768
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1769
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1769
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1770
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1770
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1771
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1771
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
gctggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1772
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1772
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Ala Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1773
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1773
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggttc aggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1774
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1774
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Gln Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1775
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1775
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcggcgct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1776
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1776
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Ala Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1777
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1777
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgtctcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1778
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1778
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Ser Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1779
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1779
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gctgaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1780
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1780
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Ala Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1781
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1781
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1782
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1782
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1783
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1783
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcgagg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1784
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1784
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Glu Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1785
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1785
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcat gaaaggcggc cgtattattc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1786
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1786
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Met Lys Gly Gly Arg Ile Ile Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1787
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1787
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1788
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1788
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1789
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1789
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gggaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1790
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1790
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Gly Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1791
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1791
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcactg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1792
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1792
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Thr Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1793
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1793
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgtgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1794
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1794
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1795
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1795
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgcttaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1796
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1796
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Leu Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1797
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1797
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc agagtatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1798
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1798
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ser Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1799
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1799
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttcatt caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1800
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1800
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Ile Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1801
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1801
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaatatgtt 1680
agcagt                                                            1686

 
<210> 1802
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1802
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Tyr Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1803
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1803
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccttctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1804
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1804
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1805
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1805
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1806
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1806
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1807
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1807
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccttgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtatttctc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1808
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1808
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Leu Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Ser Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1809
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1809
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttcacg caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1810
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1810
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Thr Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1811
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1811
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttccttggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1812
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1812
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1813
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1813
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1814
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1814
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1815
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1815
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcaccgagca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1816
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1816
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Glu Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1817
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1817
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaatgtgtt 1680
agcagt                                                            1686

 
<210> 1818
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1818
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Cys Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1819
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1819
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcggtgaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1820
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1820
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Val Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1821
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1821
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaaattgtt 1680
agcagt                                                            1686

 
<210> 1822
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1822
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Ile Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1823
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1823
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaacatgtt 1680
agcagt                                                            1686

 
<210> 1824
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1824
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln His Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1825
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1825
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaaatggtt 1680
agcagt                                                            1686

 
<210> 1826
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1826
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Met Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1827
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1827
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcactggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1828
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1828
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1829
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1829
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaacttgtt 1680
agcagt                                                            1686

 
<210> 1830
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1830
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Leu Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1831
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1831
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tactgcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaatatgtt 1680
agcagt                                                            1686

 
<210> 1832
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1832
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Cys Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Tyr Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1833
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1833
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc cagaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgctgaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acgctctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacctgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaaggcggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1834
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1834
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ala Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Cys Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1835
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1835
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1836
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1836
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1837
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1837
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttag cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1838
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1838
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Ser Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1839
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1839
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgagccgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1840
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1840
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Ser Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1841
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1841
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtcgtt 1680
agcagt                                                            1686

 
<210> 1842
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1842
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1843
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1843
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1844
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1844
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1845
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1845
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaaacaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtcgtt 1680
agcagt                                                            1686

 
<210> 1846
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1846
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1847
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1847
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaaatcgtt 1680
agcagt                                                            1686

 
<210> 1848
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1848
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Ile Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1849
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1849
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1850
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1850
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1851
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1851
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaaacaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1852
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1852
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1853
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1853
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttag cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1854
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1854
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Ser Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1855
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1855
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgagccgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttag cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgtcg aggatatcgg caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1856
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1856
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Ser Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Ser Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1857
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1857
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1858
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1858
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1859
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1859
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaaacaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1860
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1860
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1861
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1861
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaaacaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgtcg aggatatcgg caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1862
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1862
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1863
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1863
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1864
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1864
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1865
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1865
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1866
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1866
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1867
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1867
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1868
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1868
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1869
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1869
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1870
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1870
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1871
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1871
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1872
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1872
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1873
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1873
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1874
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1874
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1875
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1875
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1876
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1876
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1877
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1877
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1878
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1878
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1879
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1879
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1880
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1880
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1881
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1881
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1882
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1882
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1883
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1883
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgagccgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaaatcgtt 1680
agcagt                                                            1686

 
<210> 1884
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1884
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Ser Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Ile Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1885
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1885
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1886
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1886
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1887
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1887
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1888
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1888
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1889
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1889
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1890
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1890
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1891
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1891
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1892
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1892
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1893
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1893
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgagccgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1894
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1894
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Ser Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1895
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1895
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1896
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1896
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1897
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1897
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgca 1620
ctgatcgtcg aggatatcgg caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1898
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1898
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1899
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1899
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaaacaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1900
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1900
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1901
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1901
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaacgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1902
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1902
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Asn Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1903
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1903
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaaacaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1904
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1904
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1905
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1905
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcgt gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1906
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1906
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Cys Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1907
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1907
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttag cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc agtcgatcaa ccaagtcgtt 1680
agcagt                                                            1686

 
<210> 1908
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1908
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Ser Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ser Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1909
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1909
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc agaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtcct gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttag cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1910
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1910
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Ser Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1911
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1911
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1912
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1912
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1913
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1913
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1914
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1914
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1915
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1915
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcga aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1916
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1916
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1917
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1917
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1918
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1918
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1919
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1919
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1920
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1920
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1921
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1921
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1922
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1922
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1923
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1923
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1924
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1924
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1925
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1925
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcggt tgaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1926
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1926
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Val Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1927
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1927
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcgg aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1928
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1928
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Glu Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1929
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1929
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1930
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1930
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1931
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1931
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1932
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1932
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1933
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1933
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1934
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1934
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1935
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1935
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1936
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1936
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1937
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1937
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcga aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1938
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1938
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1939
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1939
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcgg aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1940
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1940
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Glu Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1941
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1941
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1942
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1942
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1943
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1943
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1944
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1944
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1945
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1945
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
ctgggtgcga aggcgcgcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1946
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1946
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Leu Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1947
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1947
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggttg aagtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcga aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg ttcggcaccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1948
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1948
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Glu Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1949
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1949
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1950
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1950
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1951
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1951
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1952
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1952
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1953
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1953
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1954
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1954
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1955
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1955
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcga aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcaccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1956
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1956
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1957
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1957
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcga aggcgcgcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1958
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1958
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1959
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1959
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcgg aggcgcgcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1960
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1960
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Glu Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1961
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1961
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcgg aggcgcgcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1962
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1962
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Glu Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1963
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1963
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggttg aagtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1964
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1964
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Glu Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1965
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1965
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcggc 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1966
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1966
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Gly Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1967
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1967
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1968
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1968
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1969
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1969
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag tactatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgctgat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1970
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1970
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Tyr Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Leu Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1971
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1971
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taagaagccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1972
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1972
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Lys Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1973
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1973
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcgg aggcgcgcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcaccggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1974
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1974
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Glu Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Thr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1975
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1975
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg ttcggcaccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1976
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1976
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1977
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1977
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tacggtgcga aggcgcgcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acacgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1978
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1978
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1979
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1979
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
ctgggtgcgg aggcgagcct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacgcgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca actcgctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1980
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1980
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Leu Gly Ala Glu Ala Ser Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Ala 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1981
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1981
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg cagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatt gttaacatgg gcctgaaggg gctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1982
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1982
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Ile Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1983
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1983
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tggccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatt gttaacatgg gcctgaaggg gctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1984
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1984
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Gly His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Ile Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1985
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1985
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtggccgc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaagc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacagccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaagcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcctg gttaacatgg gcctgaaggg gctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1986
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1986
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ala Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1987
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1987
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1988
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1988
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1989
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1989
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1990
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1990
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1991
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1991
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1992
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1992
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1993
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1993
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1994
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1994
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1995
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1995
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1996
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1996
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1997
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1997
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgctgaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 1998
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1998
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Leu Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 1999
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 1999
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2000
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2000
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2001
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2001
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2002
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2002
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2003
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2003
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2004
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2004
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2005
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2005
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2006
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2006
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2007
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2007
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2008
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2008
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2009
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2009
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgctgaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2010
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2010
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Leu Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2011
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2011
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgaccgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgctgaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2012
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2012
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Thr Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Leu Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2013
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2013
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2014
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2014
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2015
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2015
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2016
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2016
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2017
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2017
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2018
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2018
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2019
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2019
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgaccgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2020
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2020
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Thr Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2021
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2021
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2022
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2022
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2023
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2023
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2024
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2024
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2025
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2025
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgaccgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2026
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2026
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Thr Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2027
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2027
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2028
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2028
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2029
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2029
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2030
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2030
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2031
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2031
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2032
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2032
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2033
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2033
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2034
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2034
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2035
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2035
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2036
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2036
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2037
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2037
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgctgaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2038
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2038
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Leu Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2039
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2039
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc ggtaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2040
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2040
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Gly Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2041
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2041
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2042
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2042
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2043
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2043
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc ggtaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2044
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2044
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Gly Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2045
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2045
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgaccgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaactg 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2046
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2046
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Thr Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Leu Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2047
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2047
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgaccgc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2048
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2048
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Thr Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2049
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2049
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gctaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2050
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2050
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2051
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2051
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2052
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2052
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2053
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2053
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2054
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2054
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2055
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2055
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2056
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2056
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2057
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2057
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2058
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2058
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2059
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2059
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta aggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgg caacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2060
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2060
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Lys Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2061
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2061
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taactcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtacat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2062
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2062
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ser Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2063
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2063
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acagtctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2064
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2064
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2065
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2065
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg cagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg tagcactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2066
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2066
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2067
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2067
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2068
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2068
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2069
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2069
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg cagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2070
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2070
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2071
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2071
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gcggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtctg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgtgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2072
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2072
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Ala Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2073
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2073
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gacggacaaa gcggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaaactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2074
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2074
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Ala Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2075
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2075
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2076
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2076
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2077
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2077
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg cagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2078
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2078
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2079
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2079
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg cagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2080
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2080
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2081
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2081
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg tagcactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2082
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2082
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2083
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2083
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtctg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg tagcgctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2084
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2084
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2085
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2085
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2086
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2086
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2087
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2087
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg cagccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtctg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaaactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2088
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2088
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Gln Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2089
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2089
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcgc ctgcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2090
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2090
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Pro Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2091
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2091
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcgc gtgcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg tagcgctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2092
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2092
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Arg Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2093
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2093
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gatggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtctg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgtgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2094
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2094
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Met 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2095
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2095
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtctg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg tagcgctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgtgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2096
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2096
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Ser Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2097
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2097
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccaa caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaaactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2098
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2098
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2099
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2099
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtctg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaagctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2100
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2100
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Leu Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2101
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2101
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta ctgtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggcgcgtct gacggacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgtgg aggatatcac gaaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2102
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2102
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Val Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2103
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2103
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagca tgggtgccca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgatccgcg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2104
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2104
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Met Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Arg Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2105
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2105
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgccca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactattac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtccgcg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2106
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2106
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Ile Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Arg Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2107
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2107
atgatcattg cgagccagac ccaaaccttc accagccaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgccca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2108
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2108
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Gln Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2109
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2109
atgatcattg cgagccagac ccaaaccttc accagcagta gtccgcacac cgccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgccca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2110
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2110
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Ser Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2111
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2111
atgatcattg cgagccagac ccaaaccttc accagcagta gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgccca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgatcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2112
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2112
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Ser Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2113
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2113
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2114
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2114
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2115
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2115
atgatcattg cgagccagac cgataccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2116
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2116
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2117
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2117
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc ggtgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2118
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2118
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Val Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2119
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2119
atgatcattg cgagccagac ccaaaccttc gggagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2120
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2120
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Gly Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2121
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2121
atgatcattg atagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2122
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2122
Met Ile Ile Asp Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2123
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2123
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2124
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2124
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2125
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2125
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacca ggccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2126
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2126
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2127
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2127
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg gttcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2128
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2128
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Val Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2129
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2129
atgatcattg cgagccagac ccaaaccttc gatagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2130
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2130
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2131
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2131
atgatcattg cgagccagac ccaaaccttc accagcagga gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2132
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2132
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Arg Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2133
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2133
atgatcattg cgagccagac ccaaaccttc gcgagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2134
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2134
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Ala Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2135
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2135
atgatcattg cgagccagac ccaaaccttc agtagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2136
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2136
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Ser Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2137
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2137
atgatcattg cgagccagac ccgtaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2138
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2138
Met Ile Ile Ala Ser Gln Thr Arg Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2139
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2139
atgatcattg cgagccagac ccaaaccttc aagagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2140
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2140
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Lys Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2141
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2141
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gcgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2142
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2142
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Ala Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2143
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2143
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgcccatgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2144
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2144
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala His Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2145
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2145
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgatta gggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2146
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2146
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Arg Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2147
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2147
atgatcattg cgagccagac cgggaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2148
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2148
Met Ile Ile Ala Ser Gln Thr Gly Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2149
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2149
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgtc ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2150
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2150
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Leu 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2151
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2151
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggcgcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta tcgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat ggcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2152
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2152
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Ala Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Met Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2153
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2153
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2154
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2154
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2155
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2155
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2156
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2156
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2157
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2157
atgatcattg atagccagac ccagaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gtacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2158
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2158
Met Ile Ile Asp Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2159
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2159
atgatcattg atagccagac ccagaccttc actagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc ggtgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gtacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2160
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2160
Met Ile Ile Asp Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Val Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2161
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2161
atgatcattg atagccagac ccgtaccttc gacagccgta gtccgcacac cgccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2162
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2162
Met Ile Ile Asp Ser Gln Thr Arg Thr Phe Asp Ser Arg Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2163
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2163
atgatcattg atagccagac ccagaccttc actagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gtacccgcca actgtatctg gcggtcaagg aagtggttgg taaggctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2164
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2164
Met Ile Ile Asp Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2165
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2165
atgatcattg cgagccagac ccagaccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gtacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2166
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2166
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2167
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2167
atgatcattg atagccagac cgataccttc ggtagcaaaa gtccgcaccc cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc ggtgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taaggctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2168
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2168
Met Ile Ile Asp Ser Gln Thr Asp Thr Phe Gly Ser Lys Ser Pro His 
1               5                   10                  15      
Pro Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Val Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2169
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2169
atgatcattg atagccagac cgataccttc actagcaaaa gtccgcacac cgccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2170
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2170
Met Ile Ile Asp Ser Gln Thr Asp Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2171
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2171
atgatcattg atagccagac ccagaccttc actagcaaaa gtccgcacca ggccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gtacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgc taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2172
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2172
Met Ile Ile Asp Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Ala Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2173
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2173
atgatcattg ctagccagac ccagaccttc ggcagccgta gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taaggctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2174
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2174
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Gly Ser Arg Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2175
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2175
atgatcattg ctagccagac cgataccttc gacagccgta gtccgcacac tgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gtacccgcca actgtatctg gcggtcaagg aagtggttgg taaggctccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2176
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2176
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Arg Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2177
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2177
atgatcattg atagccagac ccgtaccttc gacagcaaaa gtccgcacac cgccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg ccgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2178
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2178
Met Ile Ile Asp Ser Gln Thr Arg Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2179
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2179
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgtgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2180
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2180
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Val Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2181
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2181
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tatggcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2182
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2182
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Met Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2183
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2183
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcagac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2184
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2184
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro Gln 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2185
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2185
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg ggatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2186
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2186
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2187
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2187
atgatcattg cgagccagac ccaaaccggg accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2188
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2188
Met Ile Ile Ala Ser Gln Thr Gln Thr Gly Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2189
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2189
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcacgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2190
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2190
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Thr Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2191
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2191
atgacgattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2192
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2192
Met Thr Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2193
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2193
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tatggcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggggccca tgcgctggcg ctgcagggcc tgaacggtac caaccatagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2194
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2194
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Met Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn His Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2195
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2195
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagaaccgaa ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcgttatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2196
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2196
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Glu Pro Asn Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Val Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2197
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2197
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2198
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2198
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2199
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2199
atgatcattg cgagccagca gcaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2200
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2200
Met Ile Ile Ala Ser Gln Gln Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2201
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2201
atggggattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2202
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2202
Met Gly Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2203
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2203
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctggag ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2204
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2204
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Glu Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2205
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2205
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gttgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2206
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2206
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2207
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2207
atgatcattg cgagcgggac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2208
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2208
Met Ile Ile Ala Ser Gly Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2209
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2209
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2210
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2210
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2211
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2211
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgtttcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2212
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2212
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ser Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2213
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2213
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcgttatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2214
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2214
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Val Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2215
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2215
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgacgac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2216
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2216
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro Thr 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2217
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2217
atgatcattg cgagccagac ccaaaccttc accagcaaaa gtccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gctctcgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2218
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2218
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Ser Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Ser Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2219
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2219
atgatcattg cgagccagac ccaaaccttc accagcaaag ggccgcacac cgccagcgac 60
agcgtggtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg cagacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgc gcacccgcca actgtatctg gcggtcaagg aagtggttgg taaggcgccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac caaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2220
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2220
Met Ile Ile Ala Ser Gln Thr Gln Thr Phe Thr Ser Lys Gly Pro His 
1               5                   10                  15      
Thr Ala Ser Asp Ser Val Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2221
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2221
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2222
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2222
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2223
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2223
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2224
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2224
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2225
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2225
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2226
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2226
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2227
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2227
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2228
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2228
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2229
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2229
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2230
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2230
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2231
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2231
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2232
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2232
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2233
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2233
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2234
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2234
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2235
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2235
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2236
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2236
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2237
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2237
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2238
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2238
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2239
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2239
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2240
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2240
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2241
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2241
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcggc 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2242
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2242
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Gly Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2243
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2243
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2244
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2244
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2245
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2245
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2246
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2246
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2247
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2247
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2248
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2248
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2249
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2249
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2250
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2250
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2251
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2251
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2252
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2252
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2253
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2253
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2254
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2254
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2255
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2255
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2256
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2256
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2257
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2257
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2258
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2258
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2259
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2259
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2260
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2260
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2261
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2261
atgatcattg atagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2262
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2262
Met Ile Ile Asp Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2263
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2263
atgattattg atagccagac ggataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2264
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2264
Met Ile Ile Asp Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2265
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2265
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaactgctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttc atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagccagctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aacaaccgat ccaagatcgt tacagcctgc gttgcctgcc gcagtatacc 960
ggcccgattg cggacggtat tgcggaaatc gcgaagcaaa tcgagattga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg ggtaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgtccg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttctacggca acagcctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc gtagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgt 1500
ctgagcccgc gcacccgtca actgtatctg gcgattaagg aagtggttgg taaggcgccg 1560
agcaaagaac gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac cagcggtggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2266
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2266
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Leu Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val His Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Gln Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Gln Pro Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Ala Asp Gly Ile Ala Glu Ile Ala Lys Gln Ile Glu Ile 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Pro Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Tyr 
            420                 425                 430         
Gly Asn Ser Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Arg 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Arg Thr Arg Gln Leu Tyr Leu Ala Ile 
            500                 505                 510         
Lys Glu Val Val Gly Lys Ala Pro Ser Lys Glu Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Ser Gly Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2267
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2267
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2268
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2268
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2269
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2269
atgattattg ctagccagac ggataccggg gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2270
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2270
Met Ile Ile Ala Ser Gln Thr Asp Thr Gly Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2271
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2271
atgattattg atagccagca ggataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2272
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2272
Met Ile Ile Asp Ser Gln Gln Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2273
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2273
atgattattg ctagccagca ggataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac cgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2274
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2274
Met Ile Ile Ala Ser Gln Gln Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2275
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2275
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2276
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2276
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2277
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2277
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2278
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2278
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2279
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2279
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
tcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2280
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2280
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ser Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2281
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2281
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
cagggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2282
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2282
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Gln Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2283
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2283
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcaaca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tatggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtgcaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2284
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2284
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro Gln 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2285
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2285
atgattattg atagccagca ggataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgt cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2286
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2286
Met Ile Ile Asp Ser Gln Gln Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Ser Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2287
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2287
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtat taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc aaaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgca ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgtccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctgac tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gctgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcgg taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2288
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2288
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Ile Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Lys Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Val Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Thr Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Leu Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Gly Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                

 
<210> 2289
<211> 1686
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2289
atgatcattg ctagccagac cgataccttc gacagcaaaa gtccgcacca ggccagcgac 60
agcaccgtta cggtgggtga taagccgctg acgatcaatg aggtgtcggc tgttgcgcgt 120
tacggtgcga aggttcgtct gaccgacaaa gaggaagtgc tgcagcgtgt taacgcgagc 180
tgcgagtggg tggcgcaggc ggttgcgaag ggtgaaccga tttacggcgt taccactggt 240
ttcggtggca tggcgaacgt ggttatcacc ccggaccagg cgaaggagct gcagaataac 300
ctgccacgct atcacaaagc gggtgcgggc caaaagctgc cgctggcgga tgttcgtgcg 360
ggtatgctgc tgcgtgcgaa cagccacctg cacggtgcga gcggtatccg tctggagctg 420
attcagcgta ttgaaacctt cctgaacgcg ggcgtgaccc cgcacgtttg cgaatttggt 480
agcatcggtg cgagcggtga cctgaccccg ctggcgtaca tcaccggtgc gctggttggt 540
ctgaacgaca gttataccgt tgattttaac ggcgaggaaa tgaccgcgcc ggaggcgctg 600
aaacgcctgg gtctggagcc gctggaactg ctgccgaagg aaggcctggc gatgatgaac 660
ggtaccgcgg tgatgaccgg tattgcggcg aactgcgttt atgatgcgca gaacctgctg 720
gcgctgagcc tgggtgcgca tgcgctggcg ctgcagggcc tgaacggtac caaccagagc 780
ttccacccgt ttattcacca gtgcaaaccg cacccgggtc aaatttggag cgcgcagcaa 840
atgctggagc tgctgcaggg cagcctgctg atccgtgacg agctggatgg tagccacgac 900
taccgcggcg aagatccgat ccaagatcgt tacagcatgc gttgcctgcc gcagtatacc 960
ggcccgattg tcgacggtat tgcagaaatc gcgaatcaaa tcgaggtcga aatcaacagc 1020
gtgaccgaca acccgctgat cgatgttgcg gggaacgcga gctaccacgg tggcaacttc 1080
atgggccagt atattggtac cagcatggac cgtctgcgtt actatctggg cctgctggcg 1140
aaacacctgg atgcccaaat cgcgctgctg accatgccgg agtttaacaa cggtctgccg 1200
gctagcctgg tgggtaacac cgaccgcatg gttaacatgg gcctgaaggg tctgcagatt 1260
accggtaaca gcatcatgcc gctgctgacc ttcctgggca acactctggg tgatcgtttc 1320
ccgacccacg cggagcagtt caaccaaaac atcaacagcc agggttttgg cagcgcgaac 1380
ctgacccgtc aaagcattga aatcttccag caatatattg cgatcgcgct gatgtttggt 1440
gtgcaaagcg ttgacctgcg taccaagaaa attgcgaacc actacgatgc gagccaatgc 1500
ctgagcccgg ccacccgcca actgtatctg gcggtcaagg aagtggttgg taagactccg 1560
agcaaagatc gtccgtatat ctggaacgac aacgagcagg cgctggatga acacattgcg 1620
ctgatcgcgg aggatatcac taaagatggc cgtattgttc aggcgatcaa ccaagtggtt 1680
agcagt                                                            1686

 
<210> 2290
<211> 562
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Tyrosine Ammonia Lyase from Stanieria cyanosphaera
 
<400> 2290
Met Ile Ile Ala Ser Gln Thr Asp Thr Phe Asp Ser Lys Ser Pro His 
1               5                   10                  15      
Gln Ala Ser Asp Ser Thr Val Thr Val Gly Asp Lys Pro Leu Thr Ile 
            20                  25                  30          
Asn Glu Val Ser Ala Val Ala Arg Tyr Gly Ala Lys Val Arg Leu Thr 
        35                  40                  45              
Asp Lys Glu Glu Val Leu Gln Arg Val Asn Ala Ser Cys Glu Trp Val 
    50                  55                  60                  
Ala Gln Ala Val Ala Lys Gly Glu Pro Ile Tyr Gly Val Thr Thr Gly 
65                  70                  75                  80  
Phe Gly Gly Met Ala Asn Val Val Ile Thr Pro Asp Gln Ala Lys Glu 
                85                  90                  95      
Leu Gln Asn Asn Leu Pro Arg Tyr His Lys Ala Gly Ala Gly Gln Lys 
            100                 105                 110         
Leu Pro Leu Ala Asp Val Arg Ala Gly Met Leu Leu Arg Ala Asn Ser 
        115                 120                 125             
His Leu His Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile Gln Arg Ile 
    130                 135                 140                 
Glu Thr Phe Leu Asn Ala Gly Val Thr Pro His Val Cys Glu Phe Gly 
145                 150                 155                 160 
Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr Ile Thr Gly 
                165                 170                 175     
Ala Leu Val Gly Leu Asn Asp Ser Tyr Thr Val Asp Phe Asn Gly Glu 
            180                 185                 190         
Glu Met Thr Ala Pro Glu Ala Leu Lys Arg Leu Gly Leu Glu Pro Leu 
        195                 200                 205             
Glu Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly Thr Ala Val 
    210                 215                 220                 
Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Ala Gln Asn Leu Leu 
225                 230                 235                 240 
Ala Leu Ser Leu Gly Ala His Ala Leu Ala Leu Gln Gly Leu Asn Gly 
                245                 250                 255     
Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Cys Lys Pro His Pro 
            260                 265                 270         
Gly Gln Ile Trp Ser Ala Gln Gln Met Leu Glu Leu Leu Gln Gly Ser 
        275                 280                 285             
Leu Leu Ile Arg Asp Glu Leu Asp Gly Ser His Asp Tyr Arg Gly Glu 
    290                 295                 300                 
Asp Pro Ile Gln Asp Arg Tyr Ser Met Arg Cys Leu Pro Gln Tyr Thr 
305                 310                 315                 320 
Gly Pro Ile Val Asp Gly Ile Ala Glu Ile Ala Asn Gln Ile Glu Val 
                325                 330                 335     
Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val Ala Gly Asn 
            340                 345                 350         
Ala Ser Tyr His Gly Gly Asn Phe Met Gly Gln Tyr Ile Gly Thr Ser 
        355                 360                 365             
Met Asp Arg Leu Arg Tyr Tyr Leu Gly Leu Leu Ala Lys His Leu Asp 
    370                 375                 380                 
Ala Gln Ile Ala Leu Leu Thr Met Pro Glu Phe Asn Asn Gly Leu Pro 
385                 390                 395                 400 
Ala Ser Leu Val Gly Asn Thr Asp Arg Met Val Asn Met Gly Leu Lys 
                405                 410                 415     
Gly Leu Gln Ile Thr Gly Asn Ser Ile Met Pro Leu Leu Thr Phe Leu 
            420                 425                 430         
Gly Asn Thr Leu Gly Asp Arg Phe Pro Thr His Ala Glu Gln Phe Asn 
        435                 440                 445             
Gln Asn Ile Asn Ser Gln Gly Phe Gly Ser Ala Asn Leu Thr Arg Gln 
    450                 455                 460                 
Ser Ile Glu Ile Phe Gln Gln Tyr Ile Ala Ile Ala Leu Met Phe Gly 
465                 470                 475                 480 
Val Gln Ser Val Asp Leu Arg Thr Lys Lys Ile Ala Asn His Tyr Asp 
                485                 490                 495     
Ala Ser Gln Cys Leu Ser Pro Ala Thr Arg Gln Leu Tyr Leu Ala Val 
            500                 505                 510         
Lys Glu Val Val Gly Lys Thr Pro Ser Lys Asp Arg Pro Tyr Ile Trp 
        515                 520                 525             
Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Leu Ile Ala Glu 
    530                 535                 540                 
Asp Ile Thr Lys Asp Gly Arg Ile Val Gln Ala Ile Asn Gln Val Val 
545                 550                 555                 560 
Ser Ser                                                         
                                                                
                                                 