                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       Durr, Eberhard
       Liu, Yaping
       Chen, Zhifeng
       Fridman, Arthur
 
<120>  METHOD FOR IDENTIFYING HIGH AFFINITY MONOCLONAL ANTIBODY HEAVY 
       AND LIGHT CHAIN PAIRS FROM HIGH THROUGHPUT SCREENS OF B-CELL AND HYBRIDOMA 
       LIBRARIES

<130>  24677

<150>  US 62/777,842
<151>  2018-12-11

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  123
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Gly Ser 
            20                  25                  30          


His Met His Trp Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly His Ile Arg Asn Ile Ala Asp Gly Ser Ala Thr Ala Tyr Ser Ala 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Thr Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Glu Met Asn Ser Leu Arg Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ser Arg Gln Thr Leu Ser Cys His Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Ala Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120             


<210>  2
<211>  136
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Pro Arg Arg Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Val Ser Gly Gly Ser Val Arg Ser Gly 
            20                  25                  30          


Asn Tyr Tyr Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Tyr Ile Phe Asp Ser Gly Thr Thr Asp Tyr Asn Pro Ser 
    50                  55                  60                  


Leu Lys Ser Arg Val Thr Met Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  


Ser Leu Gln Leu Arg Ser Val Thr Thr Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Val Arg Glu Glu Gly Lys Leu Gly Asp Cys Ser Asp Gly Ser Cys 
            100                 105                 110         


Arg Arg Lys Tyr Phe Trp Phe Asp Pro Trp Gly Gln Gly Thr Leu Val 
        115                 120                 125             


Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135     


<210>  3
<211>  131
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  3

Glu Val Gln Leu Val Gln Ser Gly Gly Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Asn Tyr 
            20                  25                  30          


Trp Ile Ser Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Lys Ile Asp Pro Ser Asp Ser Tyr Thr Lys Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly His Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg His Gly Ser Arg Asp Val Val Glu Val Pro Ala Ala Phe Asn 
            100                 105                 110         


Trp Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
        115                 120                 125             


Ser Thr Lys 
    130     


<210>  4
<211>  110
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  4

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Tyr Gly Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr His Ala Ser Ile Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Pro Tyr Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys Arg 
            100                 105                 110 


<210>  5
<211>  113
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  5

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Ile Ile Thr Cys Arg Ala Ser Gln Asn Ile Asn Arg Asn 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Gln Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Ser Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Gly Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ser Ser Thr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Ile Gly Lys 
            100                 105                 110         


Val 
    


<210>  6
<211>  114
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  6

Gln Ser Ala Leu Thr Gln Pro Arg Ala Val Ser Gly Ser Pro Gly Gln 
1               5                   10                  15      


Ser Val Thr Ile Ser Cys Thr Gly Thr Ser Ser Asn Val Gly Arg Tyr 
            20                  25                  30          


Asn Tyr Val Ser Trp Tyr Gln Gln Arg Pro Gly Lys Ala Pro Thr Leu 
        35                  40                  45              


Met Ile Tyr Asp Val Ser Glu Arg Pro Ser Gly Val Pro Asp Arg Phe 
    50                  55                  60                  


Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu 
65                  70                  75                  80  


Gln Ala Asp Asp Glu Ala Asp Tyr Tyr Cys Cys Ser Tyr Ala Gly Ser 
                85                  90                  95      


Tyr Thr Leu Leu Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln 
            100                 105                 110         


Pro Lys 
        


<210>  7
<211>  129
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  7

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Phe Thr Tyr Ala 
            20                  25                  30          


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
        35                  40                  45              


Ala Ile Ser Glu Thr Gly Asp Tyr Ile Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Tyr Ser Lys Ser Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Gly Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Asp Ser Cys Ser His Tyr Asp Cys Tyr Thr Arg Gly Phe Gly Phe 
            100                 105                 110         


Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr 
        115                 120                 125             


Lys 
    


<210>  8
<211>  126
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Arg Lys Pro Gly Ser 
1               5                   10                  15      


Ser Met Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Thr Tyr 
            20                  25                  30          


Gly Phe His Trp Val Arg Glu Ala Pro Gly Gln Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Gly Ile Met Val Val Phe Asp Thr Thr Tyr Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Phe Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Met Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Pro Tyr Tyr Asp Tyr Glu Gln Gly Gln Trp Asp Val Trp 
            100                 105                 110         


Gly Lys Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125     


<210>  9
<211>  108
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  9

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ala Asn Ser Phe Pro Val 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Arg 
            100                 105             


<210>  10
<211>  114
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  10

Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ser Asn 
            20                  25                  30          


Thr Val Asn Trp Tyr Gln His Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile His Asn Asn Asp Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Ala Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln 
65                  70                  75                  80  


Ser Asp Asp Glu Ala Asp Tyr Tyr Cys Ala Val Trp Asp Asp Ser Leu 
                85                  90                  95      


Glu Gly Gly Leu Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln 
            100                 105                 110         


Pro Lys 
        


<210>  11
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  11

Gln Ala Val Leu Thr Gln Pro Ser Ser Leu Ser Ala Ser Pro Gly Ala 
1               5                   10                  15      


Ser Ala Ser Leu Thr Cys Thr Leu His Ser Gly Ile Asn Val Ala Thr 
            20                  25                  30          


Tyr Arg Ile Phe Trp Tyr Gln Gln Lys Pro Gly Ser Pro Pro His Tyr 
        35                  40                  45              


Leu Leu Arg Tyr Lys Ser Asp Ser Asp Lys Gln Gln Gly Pro Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Gly Ser Lys Asp Ala Ser Ala Asn Ala Gly Ile 
65                  70                  75                  80  


Leu Leu Ile Ser Gly Leu Gln Ser Glu Asp Glu Ala Asn Tyr Tyr Cys 
                85                  90                  95      


Met Ile Trp His Asp Ser Gly Trp Val Phe Gly Gly Gly Thr Lys Leu 
            100                 105                 110         


Thr Val Leu Gly Gln Pro Lys 
        115                 


<210>  12
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  12

Ala Ile Ser Gly Leu Gln Ser Asp Asp Glu Ala Asp 
1               5                   10          


<210>  13
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  13

Glu Gly Gly Leu Phe Gly Gly Gly Thr Lys Leu 
1               5                   10      


<210>  14
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  14

Leu Ile His Asn Asn Asp Gln Arg Pro Ser Gly Val Pro Asp Arg Phe 
1               5                   10                  15      


<210>  15
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  15

Leu Ile His Asn Asn Asp Gln Arg Pro Ser Gly Val Pro Asp Arg Phe 
1               5                   10                  15      


Ser Ala Ser Lys Ser Gly Thr Ser Ala Ser Leu 
            20                  25          


<210>  16
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  16

Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ser Asn 
1               5                   10              


<210>  17
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  17

Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Thr 
1               5                   10              


<210>  18
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  18

Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln Thr Val Thr 
1               5                   10                  15  


<210>  19
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  19

Tyr Gln His Leu Pro Gly Thr Ala Pro Lys Leu 
1               5                   10      


<210>  20
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  20

Asp Ile Trp Gly Gln Gly Thr Met 
1               5               


<210>  21
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  21

Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu 
1               5                   10              


<210>  22
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  22

Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
1               5                   10      


<210>  23
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  23

Tyr Ala Asp Ser Val Lys Gly Arg Phe 
1               5                   


<210>  24
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  24

Tyr Leu Gln Met Asn Ser Leu Arg Ala Gly Asp Thr Ala Val 
1               5                   10                  


<210>  25
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  25

Tyr Tyr Cys Ala Lys Asp Ser Cys Ser His Tyr Asp Cys 
1               5                   10              


<210>  26
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  26

Ile Ser Arg Leu Glu Pro Glu Asp Phe 
1               5                   


<210>  27
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  27

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu 
1               5                   10                  


<210>  28
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  28

Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu 
1               5                   10      


<210>  29
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  29

Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser 
1               5                   10                  15      


Cys Arg Ala Ser Gln Ser Val Tyr Gly Ser Tyr Leu Ala 
            20                  25                  


<210>  30
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  30

Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu 
1               5                   10  


<210>  31
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  31

Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Pro Tyr Thr Phe Gly Pro Gly 
1               5                   10                  15      


Thr 
    


<210>  32
<211>  18
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  32

Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr His Ala Ser Ile Arg 
1               5                   10                  15      


Ala Thr 
        


<210>  33
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  33

Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser 
1               5                   10      


<210>  34
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  34

Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Pro Tyr Thr 
1               5                   10          


<210>  35
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  35

Arg Phe Ser Gly Ser Gly Ser 
1               5           


<210>  36
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  36

Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Tyr Gly Ser Tyr 
1               5                   10                  15      


Leu 
    


<210>  37
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  37

Thr Ile Ser Arg Asp Asp Ser Thr Asn Thr Ala Tyr 
1               5                   10          


<210>  38
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  38

Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
1               5                   10                  


<210>  39
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  39

Val Gly His Ile Arg Asn Ile Ala Asp Gly Ser Ala Thr Ala Tyr Ser 
1               5                   10                  15      


Ala Ser Val Lys 
            20  


<210>  40
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  40

Thr Asn Thr Ala Tyr Leu Glu Met Asn Ser Leu Arg Thr 
1               5                   10              


<210>  41
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  41

Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
1               5                   10                  15      


Cys Ala Ala Ser Gly Phe 
            20          


<210>  42
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  42

Ser Ser Ala Ser Thr Lys 
1               5       


<210>  43
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  43

Asp Thr Ala Val Tyr Tyr Cys Ser Arg Gln Thr 
1               5                   10      


<210>  44
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  44

Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Gly Ser His Met His Trp 
1               5                   10                  15      


Val Arg Gln Ala Ser Gly Lys Gly Leu Glu Trp Val Gly 
            20                  25                  


<210>  45
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  45

Ser Gly Lys Gly Leu Glu 
1               5       


<210>  46
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  46

Gly Ser Ala Thr Ala Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile 
1               5                   10                  15      


<210>  47
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Polypeptide

<400>  47

Arg Asn Ile Ala Asp Gly Ser Ala Thr Ala Tyr Ser Ala Ser Val Lys 
1               5                   10                  15      


Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser 
            20                  25      


