                         SEQUENCE LISTING

<110>  INSERM
 
<120>  NEW ANTI TAU SINGLE DOMAIN ANTIBODY

<130>  BIO18023 BUEE

<160>  32    

<170>  PatentIn version 3.3

<210>  1
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  minimal epitope sequence of Tau

<400>  1

Ser Val Gln Ile Val Tyr Lys Pro Val 
1               5                   


<210>  2
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH of Z70

<400>  2

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  3
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  VH-CDR1 of Z70

<400>  3

Ala Thr Ser Thr Phe Asp Gly 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  VH-CDR2 of Z70

<400>  4

Tyr Glu Gln Gly Ser Tyr Thr 
1               5           


<210>  5
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  VH-CDR3 of Z70

<400>  5

Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
1               5                   10          


<210>  6
<211>  28
<212>  PRT
<213>  Artificial

<220>
<223>  VH FR1 of Z70

<400>  6

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
            20                  25              


<210>  7
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  VH-FR2 of Z70

<400>  7

Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ser 
1               5                   10                  15      


Ala Ile Ser 
            


<210>  8
<211>  39
<212>  PRT
<213>  Artificial

<220>
<223>  VH-FR3 of Z70

<400>  8

Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
1               5                   10                  15      


Ser Lys Asn Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            20                  25                  30          


Thr Ala Thr Tyr Tyr Cys Ala 
        35                  


<210>  9
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  VH-FR4 of Z70

<400>  9

Tyr Gly Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  10
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut1

<400>  10

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Glu Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  11
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut3

<400>  11

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Lys Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  12
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH-Mut5

<400>  12

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ser 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  13
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut9

<400>  13

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Ser Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Glu Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  14
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut12

<400>  14

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Cys Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Glu Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  15
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut14

<400>  15

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Ile 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Gly Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Glu Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  16
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut15

<400>  16

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Gly Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Ser Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Glu Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  17
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH Mut20

<400>  17

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Lys Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Glu Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  18
<211>  46
<212>  PRT
<213>  Artificial

<220>
<223>  Tau[273-318]

<400>  18

Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln 
1               5                   10                  15      


Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly 
            20                  25                  30          


Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val 
        35                  40                  45      


<210>  19
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  PHF6 peptide

<400>  19

Val Gln Ile Val Tyr Lys 
1               5       


<210>  20
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  VH of AbX


<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  S or C

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  T or C

<220>
<221>  MISC_FEATURE
<222>  (47)..(47)
<223>  R or K

<220>
<221>  MISC_FEATURE
<222>  (56)..(56)
<223>  E or G

<220>
<221>  MISC_FEATURE
<222>  (90)..(90)
<223>  R or G

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  T or S

<220>
<221>  MISC_FEATURE
<222>  (101)..(101)
<223>  P or S

<220>
<221>  MISC_FEATURE
<222>  (114)..(114)
<223>  G or E

<220>
<221>  MISC_FEATURE
<222>  (115)..(115)
<223>  G or E

<400>  20

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Xaa Cys Ala Ala Ser Gly Ala Thr Ser Xaa 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Xaa Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Xaa Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Xaa Ala Glu Asp Thr Ala Xaa 
                85                  90                  95      


Tyr Tyr Cys Ala Xaa Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Xaa Xaa Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  21
<211>  28
<212>  PRT
<213>  Artificial

<220>
<223>  VH FR1 of Abx


<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  S or C

<400>  21

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Xaa Cys Ala Ala Ser Gly 
            20                  25              


<210>  22
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  VH CDR1 of Abx


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  T or C

<400>  22

Ala Thr Ser Xaa Phe Asp Gly 
1               5           


<210>  23
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  VH FR2 of Abx


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  R or K

<400>  23

Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Xaa Glu Phe Val Ser 
1               5                   10                  15      


Ala Ile Ser 
            


<210>  24
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  VH CDR2 of Abx


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  E or G

<400>  24

Tyr Xaa Gln Gly Ser Tyr Thr 
1               5           


<210>  25
<211>  39
<212>  PRT
<213>  Artificial

<220>
<223>  VH FR3 of Abx


<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  R or G

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  T or S

<400>  25

Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
1               5                   10                  15      


Ser Lys Asn Met Val Tyr Leu Gln Met Asn Ser Leu Xaa Ala Glu Asp 
            20                  25                  30          


Thr Ala Xaa Tyr Tyr Cys Ala 
        35                  


<210>  26
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  VH CDR3 of Abx


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  P or S

<400>  26

Xaa Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
1               5                   10          


<210>  27
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  VH FR4 of Abx


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  G or E

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  G or E

<400>  27

Tyr Xaa Xaa Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10          


<210>  28
<211>  126
<212>  PRT
<213>  Artificial

<220>
<223>  VH of E4-1 antibody

<400>  28

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Phe Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala 
        115                 120                 125     


<210>  29
<211>  379
<212>  PRT
<213>  Artificial

<220>
<223>  Minibody Z70

<400>  29

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ile Ser Ala Met Ala Glu Val Gln Leu Gln Ala Ser 
            20                  25                  30          


Gly Gly Val Phe Val Gln Ser Gly Gly Ser Leu Arg Leu Ser Cys Ala 
        35                  40                  45              


Ala Ser Gly Ala Thr Ser Thr Phe Asp Gly Met Gly Trp Phe Arg Gln 
    50                  55                  60                  


Ala Pro Gly Lys Glu Arg Glu Phe Val Ser Ala Ile Ser Tyr Glu Gln 
65                  70                  75                  80  


Gly Ser Tyr Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile 
                85                  90                  95      


Ser Arg Asp Asn Ser Lys Asn Met Val Tyr Leu Gln Met Asn Ser Leu 
            100                 105                 110         


Arg Ala Glu Asp Thr Ala Thr Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly 
        115                 120                 125             


Asp Leu Tyr Ala Phe Asp Ser Tyr Gly Gly Gln Gly Thr Gln Val Thr 
    130                 135                 140                 


Val Ser Ser Ala Ala Ala Arg Ser Pro Pro Leu Lys Glu Cys Pro Pro 
145                 150                 155                 160 


Cys Ala Ala Pro Asp Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro 
                165                 170                 175     


Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Met Val Thr 
            180                 185                 190         


Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser 
        195                 200                 205             


Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His 
    210                 215                 220                 


Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile 
225                 230                 235                 240 


Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn 
                245                 250                 255     


Asn Arg Ala Leu Pro Ser Pro Ile Glu Lys Thr Ile Ser Lys Pro Arg 
            260                 265                 270         


Gly Pro Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Ala Glu 
        275                 280                 285             


Glu Met Thr Lys Lys Glu Phe Ser Leu Thr Cys Met Ile Thr Gly Phe 
    290                 295                 300                 


Leu Pro Ala Glu Ile Ala Val Asp Trp Thr Ser Asn Gly Arg Thr Glu 
305                 310                 315                 320 


Gln Asn Tyr Lys Asn Thr Ala Thr Val Leu Asp Ser Asp Gly Ser Tyr 
                325                 330                 335     


Phe Met Tyr Ser Lys Leu Arg Val Gln Lys Ser Thr Trp Glu Arg Gly 
            340                 345                 350         


Ser Leu Phe Ala Cys Ser Val Val His Glu Gly Leu His Asn His Leu 
        355                 360                 365             


Thr Thr Lys Thr Ile Ser Arg Ser Leu Gly Lys 
    370                 375                 


<210>  30
<211>  126
<212>  PRT
<213>  Artificial

<220>
<223>  VH of Z70 antibody (figure 2B)

<400>  30

Met Ala Glu Val Gln Leu Gln Ala Ser Gly Gly Val Phe Val Gln Ser 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ala Thr Ser Thr 
            20                  25                  30          


Phe Asp Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ser Ala Ile Ser Tyr Glu Gln Gly Ser Tyr Thr Tyr Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn 
65                  70                  75                  80  


Met Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr 
                85                  90                  95      


Tyr Tyr Cys Ala Pro Ala Tyr Glu Gly Asp Leu Tyr Ala Phe Asp Ser 
            100                 105                 110         


Tyr Gly Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala 
        115                 120                 125     


<210>  31
<211>  372
<212>  DNA
<213>  Artificial

<220>
<223>  VH of Z70 antibody

<400>  31
atggcggaag tgcagctgca ggcttccggg ggagtatttg tgcagtcggg ggggtcattg     60

cgactgagct gcgccgcatc cggagcaact tcaacatttg acggtatggg ctggtttcgt    120

caggcccctg gcaaggagag agagttcgtt tccgccatct cctacgaaca agggtcgtat    180

acatactacg ctgacagcgt aaagggaaga tttacaatta gccgggataa ctccaaaaac    240

atggtctatc tccagatgaa cagcctcagg gccgaggaca cagctacgta ttactgtgca    300

cctgcatatg agggtgacct gtatgcattt gactcgtacg ggggacaggg gacgcaggta    360

actgtgagta gc                                                        372


<210>  32
<211>  4571
<212>  DNA
<213>  Artificial

<220>
<223>  plasmid sequence with Z70 minibody

<400>  32
cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc     60

cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg    120

gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa    180

ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga    240

acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga    300

ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga    360

actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg    420

ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct    480

caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg    540

gcgcctacct gagatcaccg gcgaaggagg gccaccatgt acaggatgca actcctgtct    600

tgcattgcac taagtcttgc acttgtcacg aattcgatat cggccatggc ggaagtgcag    660

ctgcaggctt ccgggggagt atttgtgcag tcgggggggt cattgcgact gagctgcgcc    720

gcatccggag caacttcaac atttgacggt atgggctggt ttcgtcaggc ccctggcaag    780

gagagagagt tcgtttccgc catctcctac gaacaagggt cgtatacata ctacgctgac    840

agcgtaaagg gaagatttac aattagccgg gataactcca aaaacatggt ctatctccag    900

atgaacagcc tcagggccga ggacacagct acgtattact gtgcacctgc atatgagggt    960

gacctgtatg catttgactc gtacggggga caggggacgc aggtaactgt gagtagcgcg   1020

gccgctagat ctcctccact caaagagtgt cccccatgcg cagctccaga cctcttgggt   1080

ggaccatccg tcttcatctt ccctccaaag atcaaggatg tactcatgat ctccctgagc   1140

cctatggtca catgtgtggt ggtggatgtg agcgaggatg acccagacgt ccagatcagc   1200

tggtttgtga acaacgtgga agtacacaca gctcagacac aaacccatag agaggattac   1260

aacagtactc tccgggtggt cagtgccctc cccatccagc accaggactg gatgagtggc   1320

aaggagttca aatgcaaggt caacaacaga gccctcccat cccccatcga gaaaaccatc   1380

tcaaaaccca gagggccagt aagagctcca caggtatatg tcttgcctcc accagcagaa   1440

gagatgacta agaaagagtt cagtctgacc tgcatgatca caggcttctt acctgccgaa   1500

attgctgtgg actggaccag caatgggcgt acagagcaaa actacaagaa caccgcaaca   1560

gtcctggact ctgatggttc ttacttcatg tacagcaagc tcagagtaca aaagagcact   1620

tgggaaagag gaagtctttt cgcctgctca gtggtccacg agggtctgca caatcacctt   1680

acgactaaga ccatctcccg gtctctgggt aaatgagcta gctggccaga catgataaga   1740

tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt   1800

gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac   1860

aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa   1920

agcaagtaaa acctctacaa atgtggtatg gaattaattc taaaatacag catagcaaaa   1980

ctttaacctc caaatcaagc ctctacttga atccttttct gagggatgaa taaggcatag   2040

gcatcagggg ctgttgccaa tgtgcattag ctgtttgcag cctcaccttc tttcatggag   2100

tttaagatat agtgtatttt cccaaggttt gaactagctc ttcatttctt tatgttttaa   2160

atgcactgac ctcccacatt ccctttttag taaaatattc agaaataatt taaatacatc   2220

attgcaatga aaataaatgt tttttattag gcagaatcca gatgctcaag gcccttcata   2280

atatccccca gtttagtagt tggacttagg gaacaaagga acctttaata gaaattggac   2340

agcaagaaag cgagcttcta gcttatcctc agtcctgctc ctctgccaca aagtgcacgc   2400

agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg   2460

tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt   2520

acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt   2580

cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct   2640

ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt   2700

cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catgatggct cctcctgtca   2760

ggagaggaaa gagaagaagg ttagtacaat tgctatagtg agttgtatta tactatgcag   2820

atatactatg ccaatgatta attgtcaaac tagggctgca gggttcatag tgccactttt   2880

cctgcactgc cccatctcct gcccaccctt tcccaggcat agacagtcag tgacttacca   2940

aactcacagg agggagaagg cagaagcttg agacagaccc gcgggaccgc cgaactgcga   3000

ggggacgtgg ctagggcggc ttcttttatg gtgcgccggc cctcggaggc agggcgctcg   3060

gggaggccta gcggccaatc tgcggtggca ggaggcgggg ccgaaggccg tgcctgacca   3120

atccggagca cataggagtc tcagcccccc gccccaaagc aaggggaagt cacgcgcctg   3180

tagcgccagc gtgttgtgaa atgggggctt gggggggttg gggccctgac tagtcaaaac   3240

aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat   3300

ccacgcccat tgatgtactg ccaaaaccgc atcatcatgg taatagcgat gactaatacg   3360

tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg   3420

ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt   3480

actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct   3540

attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc   3600

ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ctgcaggtta attaagaaca   3660

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt   3720

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc   3780

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct   3840

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg   3900

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca   3960

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact   4020

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta   4080

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta   4140

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct   4200

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt   4260

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga   4320

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca   4380

tggctagtta attaacattt aaatcagcgg ccggccgcaa taaaatatct ttattttcat   4440

tacatctgtg tgttggtttt ttgtgtgaat cgtaactaac atacgctctc catcaaaaca   4500

aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa   4560

catttctcta t                                                        4571


