                         SEQUENCE LISTING

<110>  Consejo Superior de Investigaciones Cientficas (CSIC)
 
<120>  CHIMERIC PROTEIN SWITCH FOR THE OPTOGENETIC CONTROL OF 
       AMYLOIDOGENESIS

<130>  PCT1641.1408

<150>  EP18382882
<151>  2018-12-03

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  285
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LOV543m3-WH1 chimera

<400>  1

Met Gly Ser Ser His His His His His His His His His His Ser Ser 
1               5                   10                  15      


Gly Leu Val Pro Arg Gly Ser Leu Ala Thr Thr Leu Glu Arg Ile Glu 
            20                  25                  30          


Lys Asn Phe Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile 
        35                  40                  45              


Phe Ala Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu 
    50                  55                  60                  


Ile Leu Gly Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg 
65                  70                  75                  80  


Ala Thr Val Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val 
                85                  90                  95      


Thr Val Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn 
            100                 105                 110         


Leu Phe His Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr 
        115                 120                 125             


Phe Ile Gly Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala 
    130                 135                 140                 


Glu Arg Glu Ala Val Met Glu Ala Lys Lys Thr Ala Glu Asn Ile Asp 
145                 150                 155                 160 


Glu Ala Ala Lys Leu Ile Glu Ser Ser His Thr Leu Thr Leu Asn Glu 
                165                 170                 175     


Lys Arg Leu Val Leu Cys Ala Ala Ser Leu Ile Asp Ser Arg Lys Pro 
            180                 185                 190         


Leu Pro Lys Asp Gly Tyr Leu Thr Ile Arg Ala Asp Thr Phe Ala Glu 
        195                 200                 205             


Val Phe Gly Ile Asp Val Lys His Ala Tyr Ala Ala Leu Asp Asp Ala 
    210                 215                 220                 


Ala Thr Lys Leu Phe Asn Arg Asp Ile Arg Arg Tyr Val Lys Gly Lys 
225                 230                 235                 240 


Val Val Glu Arg Met Arg Trp Val Phe His Val Lys Tyr Arg Glu Gly 
                245                 250                 255     


Gln Gly Cys Val Glu Leu Gly Phe Ser Pro Thr Ile Ile Pro His Leu 
            260                 265                 270         


Thr Met Leu His Lys Glu Phe Thr Ser Tyr Gln Leu Lys 
        275                 280                 285 


<210>  2
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mCherry fluorescent protein probe

<400>  2

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  3
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LOV543m3-WH1-mCherry chimera

<400>  3

Met Gly Ser Ser His His His His His His His His His His Ser Ser 
1               5                   10                  15      


Gly Leu Val Pro Arg Gly Ser Leu Ala Thr Thr Leu Glu Arg Ile Glu 
            20                  25                  30          


Lys Asn Phe Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile 
        35                  40                  45              


Phe Ala Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu 
    50                  55                  60                  


Ile Leu Gly Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg 
65                  70                  75                  80  


Ala Thr Val Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val 
                85                  90                  95      


Thr Val Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn 
            100                 105                 110         


Leu Phe His Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr 
        115                 120                 125             


Phe Ile Gly Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala 
    130                 135                 140                 


Glu Arg Glu Ala Val Met Glu Ala Lys Lys Thr Ala Glu Asn Ile Asp 
145                 150                 155                 160 


Glu Ala Ala Lys Leu Ile Glu Ser Ser His Thr Leu Thr Leu Asn Glu 
                165                 170                 175     


Lys Arg Leu Val Leu Cys Ala Ala Ser Leu Ile Asp Ser Arg Lys Pro 
            180                 185                 190         


Leu Pro Lys Asp Gly Tyr Leu Thr Ile Arg Ala Asp Thr Phe Ala Glu 
        195                 200                 205             


Val Phe Gly Ile Asp Val Lys His Ala Tyr Ala Ala Leu Asp Asp Ala 
    210                 215                 220                 


Ala Thr Lys Leu Phe Asn Arg Asp Ile Arg Arg Tyr Val Lys Gly Lys 
225                 230                 235                 240 


Val Val Glu Arg Met Arg Trp Val Phe His Val Lys Tyr Arg Glu Gly 
                245                 250                 255     


Gln Gly Cys Val Glu Leu Gly Phe Ser Pro Thr Ile Ile Pro His Leu 
            260                 265                 270         


Thr Met Leu His Lys Glu Phe Thr Ser Tyr Gln Leu Lys Gly Ser Ser 
        275                 280                 285             


Gly Ser Ser Gly Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile 
    290                 295                 300                 


Ile Lys Glu Phe Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn 
305                 310                 315                 320 


Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu 
                325                 330                 335     


Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro 
            340                 345                 350         


Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala 
        355                 360                 365             


Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe 
    370                 375                 380                 


Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly 
385                 390                 395                 400 


Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile 
                405                 410                 415     


Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val 
            420                 425                 430         


Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr 
        435                 440                 445             


Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu 
    450                 455                 460                 


Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala 
465                 470                 475                 480 


Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu 
                485                 490                 495     


Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu 
            500                 505                 510         


Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
        515                 520                 525             


<210>  4
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LOV543m3 chimera

<400>  4

Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp 
1               5                   10                  15      


Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu 
            20                  25                  30          


Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg 
        35                  40                  45              


Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg 
    50                  55                  60                  


Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr 
65                  70                  75                  80  


Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met 
                85                  90                  95      


Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp 
            100                 105                 110         


Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Ala Val Met Glu 
        115                 120                 125             


Ala Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala 
    130                 135                 140 


<210>  5
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RepA-WH1 protein

<400>  5

Lys Leu Ile Glu Ser Ser His Thr Leu Thr Leu Asn Glu Lys Arg Leu 
1               5                   10                  15      


Val Leu Cys Ala Ala Ser Leu Ile Asp Ser Arg Lys Pro Leu Pro Lys 
            20                  25                  30          


Asp Gly Tyr Leu Thr Ile Arg Ala Asp Thr Phe Ala Glu Val Phe Gly 
        35                  40                  45              


Ile Asp Val Lys His Ala Tyr Ala Ala Leu Asp Asp Ala Ala Thr Lys 
    50                  55                  60                  


Leu Phe Asn Arg Asp Ile Arg Arg Tyr Val Lys Gly Lys Val Val Glu 
65                  70                  75                  80  


Arg Met Arg Trp Val Phe His Val Lys Tyr Arg Glu Gly Gln Gly Cys 
                85                  90                  95      


Val Glu Leu Gly Phe Ser Pro Thr Ile Ile Pro His Leu Thr Met Leu 
            100                 105                 110         


His Lys Glu Phe Thr Ser Tyr Gln Leu Lys 
        115                 120         


<210>  6
<211>  420
<212>  DNA
<213>  Avena sativa

<400>  6
cttgctacta cacttgaacg tattgagaag aactttgtca ttactgaccc acgtttgcca       60

gataatccca ttatcttcgc gtccgatagt ttcttgcagt tgacagaata ttcgcgagaa      120

gaaattctgg gtcgtaactg ccgttttctt caaggtcctg aaaccgatcg cgcgacagtg      180

cgcaaaattc gtgatgccat cgataaccaa acagaggtca ctgtacagct gattaattat      240

acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc agcctatgcg tgatcagaag      300

ggtgatgtcc agtactttat tggtgtccag ttggatggta ccgaacatgt ccgtgatgcg      360

gccgagcgtg agggtgtcat gctgattaag aaaactgcag aaaatattga tgaggcggca      420


<210>  7
<211>  426
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon composition sequence from SEQ ID NO: 6

<400>  7
atgctggcga ccaccctgga acgtattgaa aaaaactttg tgattaccga tccgcgtctg       60

ccggataacc cgattatttt tgcgagcgat agctttctgc agctgaccga atatagccgt      120

gaagaaattc tgggccgtaa ctgccgtttt ctgcagggcc cggaaaccga tcgtgcgacc      180

gtgcgtaaaa ttcgtgatgc gattgataac cagaccgaag tgaccgtgca gctgattaac      240

tataccaaaa gcggcaaaaa attttggaac ctgtttcatc tgcagccgat gcgtgatcag      300

aaaggtgatg tccagtactt tattggtgtc cagttggatg gtaccgaaca tgtccgtgat      360

gcggcggaac gtgaaggcgt gatgctgatt aaaaaaaccg cagaaaacat tgatgaagcg      420

gcgtaa                                                                 426


<210>  8
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer G528A_I532A-F

<400>  8
gtgatgcggc ggaacgtgaa gccgtgatgc tggctaaaaa aaccgcagaa aacattgat        59


<210>  9
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer G528A_I532A-R

<400>  9
atcaatgttt tctgcggttt ttttagccag catcacggct tcacgttccg ccgcatcac        59


<210>  10
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer G528A_L531E_I532A-F

<400>  10
cggaacgtga agccgtgatg gaggctaaaa aaaccgcaga aaaca                       45


<210>  11
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer G528A_L531E_I532A-R

<400>  11
tgttttctgc ggttttttta gcctccatca cggcttcacg ttccg                       45


<210>  12
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Figure 7, LOV2-WT nucleotide sequence

<400>  12
gtgcgtgatg cggcggaacg tgaaggcgtg atgctgatta aaaaaaccgc agaaaacatt       60

gat                                                                     63


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Figure 7, LOV2-WT amino acid sequence

<400>  13

Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys Lys Thr 
1               5                   10                  15      


Ala Glu Asn Ile Asp 
            20      


<210>  14
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Figure 7, LOV2-G528A+L531E+I532A (m3) nucleotide sequence

<400>  14
gtgcgtgatg cggcggaacg tgaagccgtg atggaggcta aaaaaaccgc agaaaacatt       60

gat                                                                     63


<210>  15
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Figure 7, LOV2-G528A+L531E+I532A (m3) amino acid sequence

<400>  15

Val Arg Asp Ala Ala Glu Arg Glu Ala Val Met Glu Ala Lys Lys Thr 
1               5                   10                  15      


Ala Glu Asn Ile Asp 
            20      


