                               SEQUENCE LISTING

<110> GRACELL BIOTECHNOLOGIES (SHANGHAI) CO., LTD.
      SUZHOU GRACELL BIOTECHNOLOGIES CO., LTD.
 
<120> COMPOSITIONS AND METHODS FOR IMMUNOTHERAPY

<130> 181910172FF

<140> PCT/CN2019/123684
<141> 2019-12-06

<150> PCT/CN2019/097996
<151> 2019-07-26

<150> CN 201910297171.8
<151> 2019-04-12

<150> PCT/CN2019/073252
<151> 2019-01-25

<150> CN 201811501797.8
<151> 2018-12-07

<160> 16    

<170> PatentIn version 3.5

<210> 1
<211> 493
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa       60

cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc      120

gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc      180

tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc      240

cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc      300

cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca      360

ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct      420

ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc      480

gccgttacag atc                                                         493


<210> 2

<400> 2
000


<210> 3
<211> 834
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccagaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga      120

gtcaccatca gttgcagggc aagtcaggac attagtaaat atttaaattg gtatcagcag      180

aaaccagatg gaactgttaa actcctgatc taccatacat caagattaca ctcaggagtc      240

ccatcaaggt tcagtggcag tgggtctgga acagattatt ctctcaccat tagcaacctg      300

gagcaagaag atattgccac ttacttttgc caacagggta atacgcttcc gtacacgttc      360

ggagggggga ctaagttgga aataacaggc tccacctctg gatccggcaa gcccggatct      420

ggcgagggat ccaccaaggg cgaggtgaaa ctgcaggagt caggacctgg cctggtggcg      480

ccctcacaga gcctgtccgt cacatgcact gtctcagggg tctcattacc cgactatggt      540

gtaagctgga ttcgccagcc tccacgaaag ggtctggagt ggctgggagt aatatggggt      600

agtgaaacca catactataa ttcagctctc aaatccagac tgaccatcat caaggacaac      660

tccaagagcc aagttttctt aaaaatgaac agtctgcaaa ctgatgacac agccatttac      720

tactgtgcca aacattatta ctacggtggt agctatgcta tggactactg gggtcaagga      780

acctcagtca ccgtctcctc agcggccgca gactacaaag acgatgacga caag            834


<210> 4

<400> 4
000


<210> 5
<211> 321
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 5
attgaagtta tgtatcctcc tccttaccta gacaatgaga agagcaatgg aaccattatc       60

catgtgaaag ggaaacacct ttgtccaagt cccctatttc ccggaccttc taagcccttt      120

tgggtgctgg tggtggttgg gggagtcctg gcttgctata gcttgctagt aacagtggcc      180

tttattattt tctgggtgag gagtaagagg agcaggctcc tgcacagtga ctacatgaac      240

atgactcccc gccgccccgg gcccacccgc aagcattacc agccctatgc cccaccacgc      300

gacttcgcag cctatcgctc c                                                321


<210> 6

<400> 6
000


<210> 7
<211> 336
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 7
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210> 8

<400> 8
000


<210> 9
<211> 1494
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 9
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcccagaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga      120

gtcaccatca gttgcagggc aagtcaggac attagtaaat atttaaattg gtatcagcag      180

aaaccagatg gaactgttaa actcctgatc taccatacat caagattaca ctcaggagtc      240

ccatcaaggt tcagtggcag tgggtctgga acagattatt ctctcaccat tagcaacctg      300

gagcaagaag atattgccac ttacttttgc caacagggta atacgcttcc gtacacgttc      360

ggagggggga ctaagttgga aataacaggc tccacctctg gatccggcaa gcccggatct      420

ggcgagggat ccaccaaggg cgaggtgaaa ctgcaggagt caggacctgg cctggtggcg      480

ccctcacaga gcctgtccgt cacatgcact gtctcagggg tctcattacc cgactatggt      540

gtaagctgga ttcgccagcc tccacgaaag ggtctggagt ggctgggagt aatatggggt      600

agtgaaacca catactataa ttcagctctc aaatccagac tgaccatcat caaggacaac      660

tccaagagcc aagttttctt aaaaatgaac agtctgcaaa ctgatgacac agccatttac      720

tactgtgcca aacattatta ctacggtggt agctatgcta tggactactg gggtcaagga      780

acctcagtca ccgtctcctc agcggccgca gactacaaag acgatgacga caagattgaa      840

gttatgtatc ctcctcctta cctagacaat gagaagagca atggaaccat tatccatgtg      900

aaagggaaac acctttgtcc aagtccccta tttcccggac cttctaagcc cttttgggtg      960

ctggtggtgg ttgggggagt cctggcttgc tatagcttgc tagtaacagt ggcctttatt     1020

attttctggg tgaggagtaa gaggagcagg ctcctgcaca gtgactacat gaacatgact     1080

ccccgccgcc ccgggcccac ccgcaagcat taccagccct atgccccacc acgcgacttc     1140

gcagcctatc gctccagagt gaagttcagc aggagcgcag acgcccccgc gtaccagcag     1200

ggccagaacc agctctataa cgagctcaat ctaggacgaa gagaggagta cgatgttttg     1260

gacaagagac gtggccggga ccctgagatg gggggaaagc cgagaaggaa gaaccctcag     1320

gaaggcctgt acaatgaact gcagaaagat aagatggcgg aggcctacag tgagattggg     1380

atgaaaggcg agcgccggag gggcaagggg cacgatggcc tttaccaggg tctcagtaca     1440

gccaccaagg acacctacga cgcccttcac atgcaggccc tgccccctcg ctaa           1494


<210> 10

<400> 10
000


<210> 11
<211> 1824
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 11
atggagaccc tgctgggcct gctgatcctg tggctgcagc tccagtgggt gtccagcaag       60

caggaggtga cccagatccc tgccgccctg agcgtgcccg agggcgagaa cctggtgctg      120

aactgcagct tcaccgactc cgccatctac aacctgcagt ggttccggca ggaccccggc      180

aagggcctga ccagcctgct gctgatccag agcagccagc gggagcagac cagcggacgg      240

ctgaacgcca gcctggacaa gagcagcggc cggagcaccc tgtacatcgc cgccagccag      300

cccggcgaca gcgccaccta cctgtgcgct gtgcggccta ccagcggcgg cagctacatc      360

cccaccttcg gcagaggcac cagcctgatc gtgcacccct acatccagaa ccccgacccc      420

gccgtgtacc agctgcggga cagcaagagc agcgacaagt ctgtgtgcct gttcaccgac      480

ttcgacagcc agaccaatgt gagccagagc aaggacagcg acgtgtacat caccgacaag      540

accgtgctgg acatgcggag catggacttc aagagcaaca gcgccgtggc ctggagcaac      600

aagagcgact tcgcctgcgc caacgccttc aacaacagca ttatccccga ggacaccttc      660

ttccccagcc ccgagagcag ctgcgacgtg aaactggtgg agaagagctt cgagaccgac      720

accaacctga acttccagaa cctgagcgtg atcggcttca gaatcctgct gctgaaggtg      780

gccggattca acctgctgat gaccctgcgg ctgtggagca gccttggaag cggagagggc      840

agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gagcatcggc      900

ctgctgtgct gcgccgccct gagcctgctg tgggcaggac ccgtgaacgc cggagtgacc      960

cagaccccca agttccaggt gctgaaaacc ggccagagca tgaccctgca gtgcgcccag     1020

gacatgaacc acgagtacat gagctggtat cggcaggacc ccggcatggg cctgcggctg     1080

atccactact ctgtgggagc cggaatcacc gaccagggcg aggtgcccaa cggctacaat     1140

gtgagccgga gcaccaccga ggacttcccc ctgcggctgc tgagcgctgc ccccagccag     1200

accagcgtgt acttctgcgc cagcagctat gtgggcaaca ccggcgagct gttcttcggc     1260

gagggctcca ggctgaccgt gctggaggac ctgaagaacg tgttcccccc cgaggtggcc     1320

gtgttcgagc ccagcgaggc cgagatcagc cacacccaga aggccacact ggtgtgtctg     1380

gccaccggct tctaccccga ccacgtggag ctgtcctggt gggtgaacgg caaggaggtg     1440

cacagcggcg tgtctaccga cccccagccc ctgaaggagc agcccgccct gaacgacagc     1500

cggtactgcc tgtcctccag actgagagtg agcgccacct tctggcagaa cccccggaac     1560

cacttccggt gccaggtgca gttctacggc ctgagcgaga acgacgagtg gacccaggac     1620

cgggccaagc ccgtgaccca gattgtgagc gccgaggcct ggggcagggc cgactgcggc     1680

ttcaccagcg agagctacca gcagggcgtg ctgagcgcca ccatcctgta cgagatcctg     1740

ctgggcaagg ccaccctgta cgccgtgctg gtgtctgccc tggtgctgat ggctatggtg     1800

aagcggaagg acagccgggg ctaa                                            1824


<210> 12

<400> 12
000


<210> 13
<211> 607
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Met Glu Thr Leu Leu Gly Leu Leu Ile Leu Trp Leu Gln Leu Gln Trp 
1               5                   10                  15      


Val Ser Ser Lys Gln Glu Val Thr Gln Ile Pro Ala Ala Leu Ser Val 
            20                  25                  30          


Pro Glu Gly Glu Asn Leu Val Leu Asn Cys Ser Phe Thr Asp Ser Ala 
        35                  40                  45              


Ile Tyr Asn Leu Gln Trp Phe Arg Gln Asp Pro Gly Lys Gly Leu Thr 
    50                  55                  60                  


Ser Leu Leu Leu Ile Gln Ser Ser Gln Arg Glu Gln Thr Ser Gly Arg 
65                  70                  75                  80  


Leu Asn Ala Ser Leu Asp Lys Ser Ser Gly Arg Ser Thr Leu Tyr Ile 
                85                  90                  95      


Ala Ala Ser Gln Pro Gly Asp Ser Ala Thr Tyr Leu Cys Ala Val Arg 
            100                 105                 110         


Pro Thr Ser Gly Gly Ser Tyr Ile Pro Thr Phe Gly Arg Gly Thr Ser 
        115                 120                 125             


Leu Ile Val His Pro Tyr Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln 
    130                 135                 140                 


Leu Arg Asp Ser Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp 
145                 150                 155                 160 


Phe Asp Ser Gln Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr 
                165                 170                 175     


Ile Thr Asp Lys Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser 
            180                 185                 190         


Asn Ser Ala Val Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn 
        195                 200                 205             


Ala Phe Asn Asn Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro 
    210                 215                 220                 


Glu Ser Ser Cys Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp 
225                 230                 235                 240 


Thr Asn Leu Asn Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu 
                245                 250                 255     


Leu Leu Lys Val Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp 
            260                 265                 270         


Ser Ser Leu Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
        275                 280                 285             


Asp Val Glu Glu Asn Pro Gly Pro Met Ser Ile Gly Leu Leu Cys Cys 
    290                 295                 300                 


Ala Ala Leu Ser Leu Leu Trp Ala Gly Pro Val Asn Ala Gly Val Thr 
305                 310                 315                 320 


Gln Thr Pro Lys Phe Gln Val Leu Lys Thr Gly Gln Ser Met Thr Leu 
                325                 330                 335     


Gln Cys Ala Gln Asp Met Asn His Glu Tyr Met Ser Trp Tyr Arg Gln 
            340                 345                 350         


Asp Pro Gly Met Gly Leu Arg Leu Ile His Tyr Ser Val Gly Ala Gly 
        355                 360                 365             


Ile Thr Asp Gln Gly Glu Val Pro Asn Gly Tyr Asn Val Ser Arg Ser 
    370                 375                 380                 


Thr Thr Glu Asp Phe Pro Leu Arg Leu Leu Ser Ala Ala Pro Ser Gln 
385                 390                 395                 400 


Thr Ser Val Tyr Phe Cys Ala Ser Ser Tyr Val Gly Asn Thr Gly Glu 
                405                 410                 415     


Leu Phe Phe Gly Glu Gly Ser Arg Leu Thr Val Leu Glu Asp Leu Lys 
            420                 425                 430         


Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro Ser Glu Ala Glu 
        435                 440                 445             


Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala Thr Gly Phe 
    450                 455                 460                 


Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly Lys Glu Val 
465                 470                 475                 480 


His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys Glu Gln Pro Ala 
                485                 490                 495     


Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala 
            500                 505                 510         


Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe 
        515                 520                 525             


Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg Ala Lys Pro 
    530                 535                 540                 


Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly 
545                 550                 555                 560 


Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile Leu 
                565                 570                 575     


Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser 
            580                 585                 590         


Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp Ser Arg Gly 
        595                 600                 605         


<210> 14

<400> 14
000


<210> 15
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


<210> 16
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 16
His His His His His His 
1               5       


