                         SEQUENCE LISTING

<110>  ImmuneOnco Biopharmaceuticals (Shanghai) Co., Ltd
 
<120>  RECOMBINANT PROTEIN TARGETING PD-L1 AND VEGF

<130>  55525 00021

<150>  US 62/774,849
<151>  2018-12-03

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of heavy chain of anti-PD-L1 antibody

<400>  1
gaggtgcagc tggtcgagtc tggcggcggc ctcgttcaac caggcgggag cctgcggctc       60

agctgcgccg catctggatt caccttttct gattcttgga tccactgggt tcgccaggcc      120

cctggaaagg gactggagtg ggttgcctgg atctccccat atggtggctc gacttattat      180

gccgactctg tgaaaggacg gtttactatc tccgcggaca ctagcaaaaa taccgcatac      240

ctgcagatga actctctccg cgctgaagat acagctgtgt actactgcgc aagacgtcac      300

tggcccggcg gattcgacta ttgggggcag ggcactctgg tcaccgtgtc ctccgctagc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct      660

tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca      720

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc      780

acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtatgtg      840

gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacgccacg      900

taccgtgtgg tcagcgtcct caccgtcctg caccaagact ggctgaatgg caaggagtac      960

aagtgcaagg tctccaacaa agccctccca gcccccatcg ccgcaaccat ctccaaagcc     1020

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1080

aagaaccaag tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg     1140

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac     1200

tccgacggct ccttcttcct ctattccaag ctcaccgtgg acaagagcag gtggcagcag     1260

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1320

agcctctccc tgtctccggg caaatga                                         1347


<210>  2
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of heavy chain of anti-PD-L1 antibody

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ala Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Ala Ala Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445             


<210>  3
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of heavy chain of anti-PD-L1 antibody

<400>  3
gaggtgcagc tggtcgagtc tggcggcggc ctcgttcaac caggcgggag cctgcggctc       60

agctgcgccg catctggatt caccttttct gattcttgga tccactgggt tcgccaggcc      120

cctggaaagg gactggagtg ggttgcctgg atctccccat atggtggctc gacttattat      180

gccgactctg tgaaaggacg gtttactatc tccgcggaca ctagcaaaaa taccgcatac      240

ctgcagatga actctctccg cgctgaagat acagctgtgt actactgcgc aagacgtcac      300

tggcccggcg gattcgacta ttgggggcag ggcactctgg tcaccgtgtc ctccgctagc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct      660

tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca      720

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc      780

acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtatgtg      840

gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacgccacg      900

taccgtgtgg tcagcgtcct caccgtcctg caccaagact ggctgaatgg caaggagtac      960

aagtgcaagg tctccaacaa agccctccca gcccccatcg ccgcaaccat ctccaaagcc     1020

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1080

aagaaccaag tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg     1140

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac     1200

tccgacggct ccttcttcct ctattccaag ctcaccgtgg acaagagcag gtggcagcag     1260

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1320

agcctctccc tgtctccggg ctga                                            1344


<210>  4
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of heavy chain of anti-PD-L1 antibody

<400>  4

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ala Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Ala Ala Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445         


<210>  5
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequece of light chain of anti-PD-L1 antibody

<400>  5
gatattcaaa tgacacaaag cccttcttcc ctgagcgctt ctgtgggcga ccgcgttaca       60

atcacatgca gggcaagcca ggatgtcagc actgctgtcg cttggtacca gcagaaacca      120

ggcaaggcac ctaagctcct gatctactcc gcctccttcc tgtattccgg agtcccctcc      180

cgcttttccg gctccgggtc tgggaccgat ttcaccctga ccatcagctc cctccagcct      240

gaagattttg ccacctatta ttgtcagcag tacctctatc acccagcgac ctttgggcag      300

gggacaaaag tggagatcaa gcgaactgtg gctgcaccat ctgtcttcat cttcccgcca      360

tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat      420

cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag      480

gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg      540

ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc      600

ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gttag                      645


<210>  6
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of light chain of anti-PD-L1 antibody

<400>  6

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  7
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of VEGFR1D2

<400>  7
agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat tatacacatg       60

actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat cactgttact      120

ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat ctgggacagt      180

agaaagggct tcatcatatc agctgcaacg tacaaagaaa tagggcttct gacctgtgaa      240

gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca aaccaataca      300


<210>  8
<211>  100
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of VEGFR1D2

<400>  8

Ser Asp Thr Gly Arg Pro Phe Val Glu Met Tyr Ser Glu Ile Pro Glu 
1               5                   10                  15      


Ile Ile His Met Thr Glu Gly Arg Glu Leu Val Ile Pro Cys Arg Val 
            20                  25                  30          


Thr Ser Pro Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu Asp Thr 
        35                  40                  45              


Leu Ile Pro Asp Gly Lys Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe 
    50                  55                  60                  


Ile Ile Ser Ala Ala Thr Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu 
65                  70                  75                  80  


Ala Thr Val Asn Gly His Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg 
                85                  90                  95      


Gln Thr Asn Thr 
            100 


<210>  9
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of linker

<400>  9
ggcggcggtg ggagcggcgg cggtgggagc                                        30


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of linker

<400>  10

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  11
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of linker

<400>  11
ggcggcggtg ggagcggcgg cggtgggagc ggcggcgggg gctcg                       45


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of linker

<400>  12

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  13
<211>  1689
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of heavy chain of IMM2510

<400>  13
agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat tatacacatg       60

actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat cactgttact      120

ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat ctgggacagt      180

agaaagggct tcatcatatc agctgcaacg tacaaagaaa tagggcttct gacctgtgaa      240

gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca aaccaataca      300

ggcggcggtg ggagcggcgg cggtgggagc ggcggcgggg gctcggaggt gcagctggtc      360

gagtctggcg gcggcctcgt tcaaccaggc gggagcctgc ggctcagctg cgccgcatct      420

ggattcacct tttctgattc ttggatccac tgggttcgcc aggcccctgg aaagggactg      480

gagtgggttg cctggatctc cccatatggt ggctcgactt attatgccga ctctgtgaaa      540

ggacggttta ctatctccgc ggacactagc aaaaataccg catacctgca gatgaactct      600

ctccgcgctg aagatacagc tgtgtactac tgcgcaagac gtcactggcc cggcggattc      660

gactattggg ggcagggcac tctggtcacc gtgtcctccg ctagcaccaa gggcccatcg      720

gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc      780

ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc      840

agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc      900

gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac      960

aagcccagca acaccaaggt ggacaagaga gttgagccca aatcttgtga caaaactcac     1020

acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc     1080

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg     1140

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt atgtggacgg cgtggaggtg     1200

cataatgcca agacaaagcc gcgggaggag cagtacaacg ccacgtaccg tgtggtcagc     1260

gtcctcaccg tcctgcacca agactggctg aatggcaagg agtacaagtg caaggtctcc     1320

aacaaagccc tcccagcccc catcgccgca accatctcca aagccaaagg gcagccccga     1380

gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaagtcagc     1440

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat     1500

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc     1560

ttcctctatt ccaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca     1620

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct     1680

ccgggctga                                                             1689


<210>  14
<211>  562
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of heavy chain of IMM2510

<400>  14

Ser Asp Thr Gly Arg Pro Phe Val Glu Met Tyr Ser Glu Ile Pro Glu 
1               5                   10                  15      


Ile Ile His Met Thr Glu Gly Arg Glu Leu Val Ile Pro Cys Arg Val 
            20                  25                  30          


Thr Ser Pro Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu Asp Thr 
        35                  40                  45              


Leu Ile Pro Asp Gly Lys Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe 
    50                  55                  60                  


Ile Ile Ser Ala Ala Thr Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu 
65                  70                  75                  80  


Ala Thr Val Asn Gly His Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg 
                85                  90                  95      


Gln Thr Asn Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
        115                 120                 125             


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
    130                 135                 140                 


Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
145                 150                 155                 160 


Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala 
                165                 170                 175     


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
            180                 185                 190         


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
        195                 200                 205             


Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly 
    210                 215                 220                 


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
225                 230                 235                 240 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
                245                 250                 255     


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
            260                 265                 270         


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
        275                 280                 285             


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
    290                 295                 300                 


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
305                 310                 315                 320 


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys 
                325                 330                 335     


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
            340                 345                 350         


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
        355                 360                 365             


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
    370                 375                 380                 


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
385                 390                 395                 400 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ala Thr Tyr 
                405                 410                 415     


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
            420                 425                 430         


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
        435                 440                 445             


Ala Ala Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
    450                 455                 460                 


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
465                 470                 475                 480 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
                485                 490                 495     


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
            500                 505                 510         


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
        515                 520                 525             


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
    530                 535                 540                 


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
545                 550                 555                 560 


Pro Gly 
        


<210>  15
<211>  1674
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of heavy chain of IMM25011

<400>  15
gaggtgcagc tggtcgagtc tggcggcggc ctcgttcaac caggcgggag cctgcggctc       60

agctgcgccg catctggatt caccttttct gattcttgga tccactgggt tcgccaggcc      120

cctggaaagg gactggagtg ggttgcctgg atctccccat atggtggctc gacttattat      180

gccgactctg tgaaaggacg gtttactatc tccgcggaca ctagcaaaaa taccgcatac      240

ctgcagatga actctctccg cgctgaagat acagctgtgt actactgcgc aagacgtcac      300

tggcccggcg gattcgacta ttgggggcag ggcactctgg tcaccgtgtc ctccgctagc      360

accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      420

gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac      480

tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc      540

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      600

tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct      660

tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca      720

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc      780

acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtatgtg      840

gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caactccacg      900

taccgtgtgg tcagcgtcct caccgtcctg caccaagact ggctgaatgg caaggagtac      960

aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc     1020

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1080

aagaaccaag tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg     1140

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac     1200

tccgacggct ccttcttcct ctattccaag ctcaccgtgg acaagagcag gtggcagcag     1260

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1320

agcctctccc tgtctccggg cggcggcggt gggagcggcg gcggtgggag cagtgataca     1380

ggtagacctt tcgtagagat gtacagtgaa atccccgaaa ttatacacat gactgaagga     1440

agggagctcg tcattccctg ccgggttacg tcacctaaca tcactgttac tttaaaaaag     1500

tttccacttg acactttgat ccctgatgga aaacgcataa tctgggacag tagaaagggc     1560

ttcatcatat cagctgcaac gtacaaagaa atagggcttc tgacctgtga agcaacagtc     1620

aatgggcatt tgtataagac aaactatctc acacatcgac aaaccaatac atga           1674


<210>  16
<211>  557
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of heavy chain of IMM25011

<400>  16

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
225                 230                 235                 240 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                245                 250                 255     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            260                 265                 270         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        275                 280                 285             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    290                 295                 300                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
305                 310                 315                 320 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            340                 345                 350         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        355                 360                 365             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    370                 375                 380                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
385                 390                 395                 400 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                405                 410                 415     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            420                 425                 430         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly 
        435                 440                 445             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Asp Thr Gly Arg Pro Phe 
    450                 455                 460                 


Val Glu Met Tyr Ser Glu Ile Pro Glu Ile Ile His Met Thr Glu Gly 
465                 470                 475                 480 


Arg Glu Leu Val Ile Pro Cys Arg Val Thr Ser Pro Asn Ile Thr Val 
                485                 490                 495     


Thr Leu Lys Lys Phe Pro Leu Asp Thr Leu Ile Pro Asp Gly Lys Arg 
            500                 505                 510         


Ile Ile Trp Asp Ser Arg Lys Gly Phe Ile Ile Ser Ala Ala Thr Tyr 
        515                 520                 525             


Lys Glu Ile Gly Leu Leu Thr Cys Glu Ala Thr Val Asn Gly His Leu 
    530                 535                 540                 


Tyr Lys Thr Asn Tyr Leu Thr His Arg Gln Thr Asn Thr 
545                 550                 555         


<210>  17
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of light chain of IMM25031

<400>  17
agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat tatacacatg       60

actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat cactgttact      120

ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat ctgggacagt      180

agaaagggct tcatcatatc agctgcaacg tacaaagaaa tagggcttct gacctgtgaa      240

gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca aaccaataca      300

ggcggcggtg ggagcggcgg cggtgggagc ggcggcgggg gctcggatat tcaaatgaca      360

caaagccctt cttccctgag cgcttctgtg ggcgaccgcg ttacaatcac atgcagggca      420

agccaggatg tcagcactgc tgtcgcttgg taccagcaga aaccaggcaa ggcacctaag      480

ctcctgatct actccgcctc cttcctgtat tccggagtcc cctcccgctt ttccggctcc      540

gggtctggga ccgatttcac cctgaccatc agctccctcc agcctgaaga ttttgccacc      600

tattattgtc agcagtacct ctatcaccca gcgacctttg ggcaggggac aaaagtggag      660

atcaagcgaa ctgtggctgc accatctgtc ttcatcttcc cgccatctga tgagcagttg      720

aaatctggaa ctgcctctgt tgtgtgcctg ctgaataact tctatcccag agaggccaaa      780

gtacagtgga aggtggataa cgccctccaa tcgggtaact cccaggagag tgtcacagag      840

caggacagca aggacagcac ctacagcctc agcagcaccc tgacgctgag caaagcagac      900

tacgagaaac acaaagtcta cgcctgcgaa gtcacccatc agggcctgag ctcgcccgtc      960

acaaagagct tcaacagggg agagtgttag                                       990


<210>  18
<211>  329
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of light chain of IMM25031

<400>  18

Ser Asp Thr Gly Arg Pro Phe Val Glu Met Tyr Ser Glu Ile Pro Glu 
1               5                   10                  15      


Ile Ile His Met Thr Glu Gly Arg Glu Leu Val Ile Pro Cys Arg Val 
            20                  25                  30          


Thr Ser Pro Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu Asp Thr 
        35                  40                  45              


Leu Ile Pro Asp Gly Lys Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe 
    50                  55                  60                  


Ile Ile Ser Ala Ala Thr Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu 
65                  70                  75                  80  


Ala Thr Val Asn Gly His Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg 
                85                  90                  95      


Gln Thr Asn Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            100                 105                 110         


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
        115                 120                 125             


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
    130                 135                 140                 


Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
145                 150                 155                 160 


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
                165                 170                 175     


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
            180                 185                 190         


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr 
        195                 200                 205             


His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
    210                 215                 220                 


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
225                 230                 235                 240 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
                245                 250                 255     


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
            260                 265                 270         


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
        275                 280                 285             


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
    290                 295                 300                 


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
305                 310                 315                 320 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
                325                 


<210>  19
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of signal peptide of mouse IgG1 heavy chain

<400>  19
atgggatggt catgtatcat cctttttctg gtagcaactg caactggagt acattca          57


<210>  20
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak sequence

<400>  20
gccgccacc                                                                9


<210>  21
<211>  1005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of VEGFR1-Fc

<400>  21
agtgatacag gtagaccttt cgtagagatg tacagtgaaa tccccgaaat tatacacatg       60

actgaaggaa gggagctcgt cattccctgc cgggttacgt cacctaacat cactgttact      120

ttaaaaaagt ttccacttga cactttgatc cctgatggaa aacgcataat ctgggacagt      180

agaaagggct tcatcatatc agctgcaacg tacaaagaaa tagggcttct gacctgtgaa      240

gcaacagtca atgggcattt gtataagaca aactatctca cacatcgaca aaccaataca      300

gaattcgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa      360

ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc      420

tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc      480

aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag      540

gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg      600

ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag      660

aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca      720

tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat      780

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc      840

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac      900

aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac      960

aaccactaca cgcagaagag cctctccctg tctccgggta aatga                     1005


<210>  22
<211>  334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of VEGFR1-Fc

<400>  22

Ser Asp Thr Gly Arg Pro Phe Val Glu Met Tyr Ser Glu Ile Pro Glu 
1               5                   10                  15      


Ile Ile His Met Thr Glu Gly Arg Glu Leu Val Ile Pro Cys Arg Val 
            20                  25                  30          


Thr Ser Pro Asn Ile Thr Val Thr Leu Lys Lys Phe Pro Leu Asp Thr 
        35                  40                  45              


Leu Ile Pro Asp Gly Lys Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe 
    50                  55                  60                  


Ile Ile Ser Ala Ala Thr Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu 
65                  70                  75                  80  


Ala Thr Val Asn Gly His Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg 
                85                  90                  95      


Gln Thr Asn Thr Glu Phe Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
            100                 105                 110         


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
        115                 120                 125             


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
    130                 135                 140                 


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
145                 150                 155                 160 


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
                165                 170                 175     


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
            180                 185                 190         


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
        195                 200                 205             


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
    210                 215                 220                 


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
225                 230                 235                 240 


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
                245                 250                 255     


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
            260                 265                 270         


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
        275                 280                 285             


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
    290                 295                 300                 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
305                 310                 315                 320 


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330                 

<210>  23
<211>  582
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIRP alpha D1-Linker-anti-EGFR heavy chain

<400>  23

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Ser Val Ala 
1               5                   10                  15      


Ala Gly Glu Ser Ala Ile Leu His Cys Thr Val Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Ala Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Glu Ser Thr Lys Arg Glu Asn Met Asp Phe Ser Ile Ser Ile Ser Ala 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Thr Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu Ser 
            100                 105                 110         


Val Arg Ala Lys Pro Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu 
    130                 135                 140                 


Val Gln Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe 
145                 150                 155                 160 


Ser Leu Thr Asn Tyr Gly Val His Trp Val Arg Gln Ser Pro Gly Lys 
                165                 170                 175     


Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly Asn Thr Asp Tyr 
            180                 185                 190         


Asn Thr Pro Phe Thr Ser Arg Leu Ser Ile Asn Lys Asp Asn Ser Lys 
        195                 200                 205             


Ser Gln Val Phe Phe Lys Met Asn Ser Leu Gln Ser Gln Asp Thr Ala 
    210                 215                 220                 


Ile Tyr Tyr Cys Ala Arg Ala Leu Thr Tyr Tyr Asp Tyr Glu Phe Ala 
225                 230                 235                 240 


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys 
                245                 250                 255     


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
            260                 265                 270         


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
        275                 280                 285             


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
    290                 295                 300                 


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
305                 310                 315                 320 


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
                325                 330                 335     


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro 
            340                 345                 350         


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
        355                 360                 365             


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
    370                 375                 380                 


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
385                 390                 395                 400 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
                405                 410                 415     


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
            420                 425                 430         


Ala Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
        435                 440                 445             


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
    450                 455                 460                 


Ala Pro Ile Ala Ala Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
465                 470                 475                 480 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
                485                 490                 495     


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
            500                 505                 510         


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
        515                 520                 525             


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
    530                 535                 540                 


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
545                 550                 555                 560 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
                565                 570                 575     


Ser Leu Ser Pro Gly Lys 
            580         


<210>  24
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain of anti-EGFR antibody

<400>  24

Asp Ile Leu Leu Thr Gln Ser Pro Val Ile Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Ser Ile Gly Thr Asn 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Glu Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Ser 
65                  70                  75                  80  


Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Asn Asn Asn Trp Pro Thr 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  25
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIRP alpha D1-Fc

<400>  25

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Ser Val Ala 
1               5                   10                  15      


Ala Gly Glu Ser Ala Ile Leu His Cys Thr Val Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Ala Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Glu Ser Thr Lys Arg Glu Asn Met Asp Phe Ser Ile Ser Ile Ser Ala 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Thr Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu Ser 
            100                 105                 110         


Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala Arg 
        115                 120                 125             


Ala Thr Pro Gln His Glu Phe Glu Pro Lys Ser Cys Asp Lys Thr His 
    130                 135                 140                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
145                 150                 155                 160 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                165                 170                 175     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            180                 185                 190         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        195                 200                 205             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    210                 215                 220                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
225                 230                 235                 240 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                245                 250                 255     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            260                 265                 270         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        275                 280                 285             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    290                 295                 300                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
305                 310                 315                 320 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                325                 330                 335     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            340                 345                 350         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        355                 360                 365     

