                         LISTAGEM DE SEQUNCIAS

<110>  FIOCRUZ FUNDAO OSWALDO CRUZ
	 INSTITUTO DE BIOLOGIA MOLECULAR DO PARAN - IBMP
	 UNIVERSIDADE FEDERAL DO PARAN - UFPR
 
<120>  PROTENA RECOMBINANTE, SEQUNCIA DE DNA SINTTICO, VETOR DE 
       EXPRESSO, CLULA HOSPEDEIRA, COMPOSIO, KIT PARA DIAGNSTICO DE
       RUBOLA, USO DE PELO MENOS UMA PROTENA RECOMBINANTE, E, MTODOS
       PARA PRODUZIR UMA PROTENA RECOMBINANTE E PARA DIAGNSTICO DE 
       RUBOLA"

<130>  Caso 174

<160>  10    

<170>  PatentIn verso 3.5

<210>  1
<211>  75
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Protena Rub2

<400>  1

Gln Leu Pro Phe Leu Gly His Asp Gly His His Gly Gly Thr Leu Arg 
1               5                   10                  15      


Val Gly Gln His Tyr Arg Asn Ala Ser Asp Val Leu Pro Gly His Trp 
            20                  25                  30          


Leu Gln Gly Gly Trp Gly Cys Tyr Asn Leu Ser Asp Trp His Gln Gly 
        35                  40                  45              


Thr His Val Cys His Thr Lys His Met Asp Phe Trp Cys Val Glu His 
    50                  55                  60                  


Asp Arg Pro Pro Pro Ala Thr Pro Thr Pro Leu 
65                  70                  75  


<210>  2
<211>  260
<212>  DNA
<213>  Sequncia artificial

<220>
<223>  gene sinttico Rub2

<400>  2
ccatggctgg atcccagctg ccgttcctgg gtcatgatgg ccatcacggt ggtaccctcc       60

gtgtaggcca gcactatcgc aacgcgtctg acgtgctgcc aggtcattgg ctgcagggtg      120

gctggggctg ttataacctg tccgattggc accagggcac ccacgtctgt cacaccaaac      180

acatggactt ctggtgcgtg gaacacgacc gtccaccacc ggctactcca actccgctgg      240

cgaattcctg ataaaagctt                                                  260


<210>  3
<211>  123
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Protena Rub3

<400>  3

Met Ala Ser Thr Thr Pro Ile Thr Met Glu Asp Leu Gln Lys Ala Leu 
1               5                   10                  15      


Glu Ala Gln Ser Arg Ala Leu Arg Ala Glu Leu Ala Ala Gly Ala Ser 
            20                  25                  30          


Gln Ser Arg Arg Pro Arg Pro Pro Arg Gln Arg Asp Ser Ser Thr Ser 
        35                  40                  45              


Gly Asp Asp Ser Gly Arg Asp Ser Gly Gly Pro Arg Arg Arg Arg Gly 
    50                  55                  60                  


Asn Arg Gly Arg Gly Gln Arg Arg Asp Trp Ser Arg Ala Pro Pro Pro 
65                  70                  75                  80  


Pro Glu Glu Arg Gln Glu Thr Arg Ser Gln Thr Pro Ala Pro Lys Pro 
                85                  90                  95      


Ser Arg Ala Pro Pro Gln Gln Pro Gln Pro Pro Arg Met Gln Thr Gly 
            100                 105                 110         


Arg Gly Gly Ser Ala Pro Arg Pro Glu Leu Gly 
        115                 120             


<210>  4
<211>  401
<212>  DNA
<213>  Sequncia artificial

<220>
<223>  gene sinttico Rub3

<400>  4
ccatggctgg atccgcgagc actaccccga tcactatgga ggatctccag aaagccctgg       60

aagcccagtc tcgtgcgctg cgtgctgaac tggcggcagg tgcaagccag tctcgtcgtc      120

cacgtccacc gcgtcagcgc gattcttcca cgtccggcga cgactctggt cgtgactctg      180

gcggtccgcg tcgtcgccgt ggtaaccgtg gtcgtggtca gcgccgtgat tggtctcgcg      240

ctccgccacc accggaagag cgccaggaaa ctcgctccca gaccccagcg ccgaaaccga      300

gccgcgctcc accgcagcag ccacagccgc cacgtatgca gaccggtcgt ggcggttccg      360

caccacgtcc ggaactgggc gcgaattcct gataaaagct t                          401


<210>  5
<211>  248
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Protena Rub12

<400>  5

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          


Gly Ser Gln Val Pro Pro Asp Pro Gly Asp Leu Val Glu Tyr Ile Met 
        35                  40                  45              


Asn Tyr Thr Gly Asn Gln Gln Ser Arg Trp Gly Leu Gly Ser Pro Asn 
    50                  55                  60                  


Cys His Gly Pro Asp Trp Ala Ser Pro Val Cys Gln Arg His Ser Pro 
65                  70                  75                  80  


Asp Cys Ser Arg Leu Val Gly Ala Thr Pro Glu Arg Pro Arg Leu Arg 
                85                  90                  95      


Leu Val Asp Ala Asp Asp Pro Leu Leu Arg Thr Ala Pro Gly Pro Gly 
            100                 105                 110         


Glu Val Trp Val Thr Pro Val Ile Gly Ser Gln Ala Arg Lys Cys Gly 
        115                 120                 125             


Leu His Ile Arg Ala Gly Pro Tyr Gly His Ala Thr Val Glu Met Pro 
    130                 135                 140                 


Glu Trp Ile His Ala His Thr Thr Ser Asp Pro Trp His Pro Pro Gly 
145                 150                 155                 160 


Pro Leu Gly Leu Lys Phe Lys Thr Val Arg Pro Val Gly Gly Gly Asn 
                165                 170                 175     


Cys His Leu Thr Val Asn Gly Glu Asp Leu Gly Ala Phe Pro Pro Gly 
            180                 185                 190         


Lys Phe Val Thr Ala Ala Leu Leu Asn Thr Pro Pro Pro Tyr Gln Val 
        195                 200                 205             


Ser Cys Gly Gly Glu Ser Asp Arg Ala Ser Ala Arg Val Ile Asp Pro 
    210                 215                 220                 


Ala Ala Gln Ser Phe Thr Gly Val Val Tyr Gly Thr His Thr Thr Ala 
225                 230                 235                 240 


Val Ser Glu Thr Arg Gly Gly Ser 
                245             


<210>  6
<211>  814
<212>  DNA
<213>  Sequncia artificial

<220>
<223>  gene sinttico Rub12

<400>  6
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atggctagca tgactggtgg acagcaaatg ggtcgcggat ctcaagtgcc gccggacccg      120

ggcgacctgg tggaatacat tatgaattac acgggcaatc aacaatcacg ctggggcctg      180

ggctcaccga actgtcatgg cccggattgg gcaagcccgg tttgccagcg tcacagcccg      240

gactgttctc gtctggtcgg cgctaccccg gaacgtccgc gtctgcgtct ggtggatgcg      300

gatgacccgc tgctgcgtac cgcaccgggt ccgggcgaag tgtgggttac gccggttatt      360

ggttcacaag cgcgcaaatg tggcctgcat atccgtgcag gtccgtatgg ccacgctacc      420

gtcgaaatgc cggaatggat tcatgcccac accaccagcg atccgtggca tccgccgggt      480

ccgctgggtc tgaaatttaa aaccgtccgt ccggtgggcg gtggcaactg ccacctgacg      540

gtgaatggtg aagacctggg cgcgtttccg ccgggtaaat tcgttaccgc agcactgctg      600

aataccccgc cgccgtacca agtcagttgt ggtggcgaaa gtgatcgcgc gtccgcccgt      660

gtgatcgacc cggcagctca gtcattcacc ggcgttgtct acggcaccca tacgaccgca      720

gttagcgaaa cccgtggcgg atcctgataa aagcttgcgg ccgcactcga gcaccaccac      780

caccaccact gagatccggc tgctaacaaa gccc                                  814


<210>  7
<211>  430
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Protena Rub14

<400>  7

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          


Gly Ser Gln Val Pro Pro Asp Pro Gly Asp Leu Val Glu Tyr Ile Met 
        35                  40                  45              


Asn Tyr Thr Gly Asn Gln Gln Ser Arg Trp Gly Leu Gly Ser Pro Asn 
    50                  55                  60                  


Cys His Gly Pro Asp Trp Ala Ser Pro Val Cys Gln Arg His Ser Pro 
65                  70                  75                  80  


Asp Cys Ser Arg Leu Val Gly Ala Thr Pro Glu Arg Pro Arg Leu Arg 
                85                  90                  95      


Leu Val Asp Ala Asp Asp Pro Leu Leu Arg Thr Ala Pro Gly Pro Gly 
            100                 105                 110         


Glu Val Trp Val Thr Pro Val Ile Gly Ser Gln Ala Arg Lys Cys Gly 
        115                 120                 125             


Leu His Ile Arg Ala Gly Pro Tyr Gly His Ala Thr Val Glu Met Pro 
    130                 135                 140                 


Glu Trp Ile His Ala His Thr Thr Ser Asp Pro Trp His Pro Pro Gly 
145                 150                 155                 160 


Pro Leu Gly Leu Lys Phe Lys Thr Val Arg Pro Val Gly Gly Gly Asn 
                165                 170                 175     


Cys His Leu Thr Val Asn Gly Glu Asp Leu Gly Ala Phe Pro Pro Gly 
            180                 185                 190         


Lys Phe Val Thr Ala Ala Leu Leu Asn Thr Pro Pro Pro Tyr Gln Val 
        195                 200                 205             


Ser Cys Gly Gly Glu Ser Asp Arg Ala Ser Ala Arg Val Ile Asp Pro 
    210                 215                 220                 


Ala Ala Gln Ser Phe Thr Gly Val Val Tyr Gly Thr His Thr Thr Ala 
225                 230                 235                 240 


Val Ser Glu Thr Arg Gly Gly Ser Gly Leu Gln Pro Arg Ala Asp Met 
                245                 250                 255     


Ala Ala Pro Pro Thr Leu Pro Gln Pro Pro Arg Ala His Gly Gln His 
            260                 265                 270         


Tyr Gly His His His His Gln Leu Pro Phe Leu Gly His Asp Gly His 
        275                 280                 285             


His Gly Gly Thr Leu Arg Val Gly Gln His His Arg Asn Ala Ser Asp 
    290                 295                 300                 


Val Leu Pro Gly His Trp Leu Gln Gly Gly Trp Gly Cys Tyr Asn Leu 
305                 310                 315                 320 


Ser Asp Trp His Gln Gly Thr His His Ala Gly Leu Asn Asp Ser Cys 
                325                 330                 335     


Gly Gly Phe Leu Ser Gly Cys Gly Pro Met Arg Leu Arg His Gly Ala 
            340                 345                 350         


Asp Thr Arg Cys Gly Arg Leu Ile Cys Gly Leu Ser Thr Thr Ala Gln 
        355                 360                 365             


Tyr Pro Pro Thr Arg Phe Gly Cys Ala Met Arg Trp Gly Leu Pro Pro 
    370                 375                 380                 


Trp Glu Leu Val Val Leu Thr Ala Arg His Ala Leu Ala Ala Phe Val 
385                 390                 395                 400 


Leu Leu Val Pro Trp Val Leu Ile Phe Met Val Cys Arg Arg Thr Cys 
                405                 410                 415     


Arg Arg Arg Gly Ala Ala Ala Ala Leu Thr Ala Gly Gly Ser 
            420                 425                 430 


<210>  8
<211>  1302
<212>  DNA
<213>  Sequncia artificial

<220>
<223>  gene sinttico Rub14

<400>  8
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atggctagca tgactggtgg acagcaaatg ggtcgcggat ctcaagtgcc gccggacccg      120

ggcgacctgg tggaatacat tatgaattac acgggcaatc aacaatcacg ctggggcctg      180

ggctcaccga actgtcatgg cccggattgg gcaagcccgg tttgccagcg tcacagcccg      240

gactgttctc gtctggtcgg cgctaccccg gaacgtccgc gtctgcgtct ggtggatgcg      300

gatgacccgc tgctgcgtac cgcaccgggt ccgggcgaag tgtgggttac gccggttatt      360

ggttcacaag cgcgcaaatg tggcctgcat atccgtgcag gtccgtatgg ccacgctacc      420

gtcgaaatgc cggaatggat tcatgcccac accaccagcg atccgtggca tccgccgggt      480

ccgctgggtc tgaaatttaa aaccgtccgt ccggtgggcg gtggcaactg ccacctgacg      540

gtgaatggtg aagacctggg cgcgtttccg ccgggtaaat tcgttaccgc agcactgctg      600

aataccccgc cgccgtacca agtcagttgt ggtggcgaaa gtgatcgcgc gtccgcccgt      660

gtgatcgacc cggcagctca gtcattcacc ggcgttgtct acggcaccca tacgaccgca      720

gttagcgaaa cccgtggcgg atctggcctg caaccgcgtg ccgacatggc tgccccgccg      780

acgctgccgc aaccgccgcg tgctcatggt caacactatg gtcatcatca tcatcagctg      840

ccgtttctgg gccacgatgg tcatcacggc ggtaccctgc gtgtcggcca acatcaccgc      900

aacgcaagtg acgtgctgcc gggtcattgg ctgcagggcg gttggggttg ctataacctg      960

tccgattggc accaaggcac gcatcacgca ggtctgaatg acagctgcgg cggtttcctg     1020

tctggctgtg gtccgatgcg tctgcgtcat ggcgctgata cccgttgcgg ccgcctgatt     1080

tgtggtctga gcaccacggc acagtacccg ccgacgcgtt ttggttgtgc aatgcgttgg     1140

ggtctgccgc cgtgggaact ggtggttctg accgcacgtc acgctctggc agcattcgtc     1200

ctgctggtgc cgtgggttct gatcttcatg gtgtgtcgtc gtacctgtcg tcgtcgtggt     1260

gccgccgccg ccctgaccgc tggcggatcc tgataaaagc tt                        1302


<210>  9
<211>  554
<212>  PRT
<213>  Sequncia artificial

<220>
<223>  Protena Rub17

<400>  9

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg 
            20                  25                  30          


Gly Ser Gln Val Pro Pro Asp Pro Gly Asp Leu Val Glu Tyr Ile Met 
        35                  40                  45              


Asn Tyr Thr Gly Asn Gln Gln Ser Arg Trp Gly Leu Gly Ser Pro Asn 
    50                  55                  60                  


Cys His Gly Pro Asp Trp Ala Ser Pro Val Cys Gln Arg His Ser Pro 
65                  70                  75                  80  


Asp Cys Ser Arg Leu Val Gly Ala Thr Pro Glu Arg Pro Arg Leu Arg 
                85                  90                  95      


Leu Val Asp Ala Asp Asp Pro Leu Leu Arg Thr Ala Pro Gly Pro Gly 
            100                 105                 110         


Glu Val Trp Val Thr Pro Val Ile Gly Ser Gln Ala Arg Lys Cys Gly 
        115                 120                 125             


Leu His Ile Arg Ala Gly Pro Tyr Gly His Ala Thr Val Glu Met Pro 
    130                 135                 140                 


Glu Trp Ile His Ala His Thr Thr Ser Asp Pro Trp His Pro Pro Gly 
145                 150                 155                 160 


Pro Leu Gly Leu Lys Phe Lys Thr Val Arg Pro Val Gly Gly Gly Asn 
                165                 170                 175     


Cys His Leu Thr Val Asn Gly Glu Asp Leu Gly Ala Phe Pro Pro Gly 
            180                 185                 190         


Lys Phe Val Thr Ala Ala Leu Leu Asn Thr Pro Pro Pro Tyr Gln Val 
        195                 200                 205             


Ser Cys Gly Gly Glu Ser Asp Arg Ala Ser Ala Arg Val Ile Asp Pro 
    210                 215                 220                 


Ala Ala Gln Ser Phe Thr Gly Val Val Tyr Gly Thr His Thr Thr Ala 
225                 230                 235                 240 


Val Ser Glu Thr Arg Gly Gly Ser Gly Leu Gln Pro Arg Ala Asp Met 
                245                 250                 255     


Ala Ala Pro Pro Thr Leu Pro Gln Pro Pro Arg Ala His Gly Gln His 
            260                 265                 270         


Tyr Gly His His His His Gln Leu Pro Phe Leu Gly His Asp Gly His 
        275                 280                 285             


His Gly Gly Thr Leu Arg Val Gly Gln His His Arg Asn Ala Ser Asp 
    290                 295                 300                 


Val Leu Pro Gly His Trp Leu Gln Gly Gly Trp Gly Cys Tyr Asn Leu 
305                 310                 315                 320 


Ser Asp Trp His Gln Gly Thr His His Ala Gly Leu Asn Asp Ser Cys 
                325                 330                 335     


Gly Gly Phe Leu Ser Gly Cys Gly Pro Met Arg Leu Arg His Gly Ala 
            340                 345                 350         


Asp Thr Arg Cys Gly Arg Leu Ile Cys Gly Leu Ser Thr Thr Ala Gln 
        355                 360                 365             


Tyr Pro Pro Thr Arg Phe Gly Cys Ala Met Arg Trp Gly Leu Pro Pro 
    370                 375                 380                 


Trp Glu Leu Val Val Leu Thr Ala Arg His Ala Leu Ala Ala Phe Val 
385                 390                 395                 400 


Leu Leu Val Pro Trp Val Leu Ile Phe Met Val Cys Arg Arg Thr Cys 
                405                 410                 415     


Arg Arg Arg Gly Ala Ala Ala Ala Leu Thr Ala Gly Ser Ala Ser Thr 
            420                 425                 430         


Thr Pro Ile Thr Met Glu Asp Leu Gln Lys Ala Leu Glu Ala Gln Ser 
        435                 440                 445             


Arg Ala Leu Arg Ala Glu Leu Ala Ala Gly Ala Ser Gln Ser Arg Arg 
    450                 455                 460                 


Pro Arg Pro Pro Arg Gln Arg Asp Ser Ser Thr Ser Gly Asp Asp Ser 
465                 470                 475                 480 


Gly Arg Asp Ser Gly Gly Pro Arg Arg Arg Arg Gly Asn Arg Gly Arg 
                485                 490                 495     


Gly Gln Arg Arg Asp Trp Ser Arg Ala Pro Pro Pro Pro Glu Glu Arg 
            500                 505                 510         


Gln Glu Thr Arg Ser Gln Thr Pro Ala Pro Lys Pro Ser Arg Ala Pro 
        515                 520                 525             


Pro Gln Gln Pro Gln Pro Pro Arg Met Gln Thr Gly Arg Gly Gly Ser 
    530                 535                 540                 


Ala Pro Arg Pro Glu Leu Gly Ala Asn Ser 
545                 550                 


<210>  10
<211>  1677
<212>  DNA
<213>  Sequncia artificial

<220>
<223>  gene sinttico Rub17

<400>  10
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atggctagca tgactggtgg acagcaaatg ggtcgcggat ctcaagtgcc gccggacccg      120

ggcgacctgg tggaatacat tatgaattac acgggcaatc aacaatcacg ctggggcctg      180

ggctcaccga actgtcatgg cccggattgg gcaagcccgg tttgccagcg tcacagcccg      240

gactgttctc gtctggtcgg cgctaccccg gaacgtccgc gtctgcgtct ggtggatgcg      300

gatgacccgc tgctgcgtac cgcaccgggt ccgggcgaag tgtgggttac gccggttatt      360

ggttcacaag cgcgcaaatg tggcctgcat atccgtgcag gtccgtatgg ccacgctacc      420

gtcgaaatgc cggaatggat tcatgcccac accaccagcg atccgtggca tccgccgggt      480

ccgctgggtc tgaaatttaa aaccgtccgt ccggtgggcg gtggcaactg ccacctgacg      540

gtgaatggtg aagacctggg cgcgtttccg ccgggtaaat tcgttaccgc agcactgctg      600

aataccccgc cgccgtacca agtcagttgt ggtggcgaaa gtgatcgcgc gtccgcccgt      660

gtgatcgacc cggcagctca gtcattcacc ggcgttgtct acggcaccca tacgaccgca      720

gttagcgaaa cccgtggcgg atctggcctg caaccgcgtg ccgacatggc tgccccgccg      780

acgctgccgc aaccgccgcg tgctcatggt caacactatg gtcatcatca tcatcagctg      840

ccgtttctgg gccacgatgg tcatcacggc ggtaccctgc gtgtcggcca acatcaccgc      900

aacgcaagtg acgtgctgcc gggtcattgg ctgcagggcg gttggggttg ctataacctg      960

tccgattggc accaaggcac gcatcacgca ggtctgaatg acagctgcgg cggtttcctg     1020

tctggctgtg gtccgatgcg tctgcgtcat ggcgctgata cccgttgcgg ccgcctgatt     1080

tgtggtctga gcaccacggc acagtacccg ccgacgcgtt ttggttgtgc aatgcgttgg     1140

ggtctgccgc cgtgggaact ggtggttctg accgcacgtc acgctctggc agcattcgtc     1200

ctgctggtgc cgtgggttct gatcttcatg gtgtgtcgtc gtacctgtcg tcgtcgtggt     1260

gccgccgccg ccctgaccgc tggcggatcc gcgagcacta ccccgatcac tatggaggat     1320

ctccagaaag ccctggaagc ccagtctcgt gcgctgcgtg ctgaactggc ggcaggtgca     1380

agccagtctc gtcgtccacg tccaccgcgt cagcgcgatt cttccacgtc cggcgacgac     1440

tctggtcgtg actctggcgg tccgcgtcgt cgccgtggta accgtggtcg tggtcagcgc     1500

cgtgattggt ctcgcgctcc gccaccaccg gaagagcgcc aggaaactcg ctcccagacc     1560

ccagcgccga aaccgagccg cgctccaccg cagcagccac agccgccacg tatgcagacc     1620

ggtcgtggcg gttccgcacc acgtccggaa ctgggcgcga attcctgata aaagctt        1677