                         SEQUENCE LISTING

<110>  CSL Innovation Pty Ltd
 
<120>  Method of treating neutrophilic conditions

<130>  524787PCT

<150>  US 62/774,974
<151>  2018-12-04

<150>  US 62/885,373
<151>  2019-08-12

<150>  US 62/897,487
<151>  2019-09-09

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  319
<212>  PRT
<213>  artificial

<220>
<223>  amino acids 25-335 of Homo sapiens G-CSFR (hG-CSFR) with a 
       C-terminal polyhistidine tag

<400>  1

Glu Cys Gly His Ile Ser Val Ser Ala Pro Ile Val His Leu Gly Asp 
1               5                   10                  15      


Pro Ile Thr Ala Ser Cys Ile Ile Lys Gln Asn Cys Ser His Leu Asp 
            20                  25                  30          


Pro Glu Pro Gln Ile Leu Trp Arg Leu Gly Ala Glu Leu Gln Pro Gly 
        35                  40                  45              


Gly Arg Gln Gln Arg Leu Ser Asp Gly Thr Gln Glu Ser Ile Ile Thr 
    50                  55                  60                  


Leu Pro His Leu Asn His Thr Gln Ala Phe Leu Ser Cys Ala Leu Asn 
65                  70                  75                  80  


Trp Gly Asn Ser Leu Gln Ile Leu Asp Gln Val Glu Leu Arg Ala Gly 
                85                  90                  95      


Tyr Pro Pro Ala Ile Pro His Asn Leu Ser Cys Leu Met Asn Leu Thr 
            100                 105                 110         


Thr Ser Ser Leu Ile Cys Gln Trp Glu Pro Gly Pro Glu Thr His Leu 
        115                 120                 125             


Pro Thr Ser Phe Thr Leu Lys Ser Phe Lys Ser Arg Gly Asn Cys Gln 
    130                 135                 140                 


Thr Gln Gly Asp Ser Ile Leu Asp Cys Val Pro Lys Asp Gly Gln Ser 
145                 150                 155                 160 


His Cys Ser Ile Pro Arg Lys His Leu Leu Leu Tyr Gln Asn Met Gly 
                165                 170                 175     


Ile Trp Val Gln Ala Glu Asn Ala Leu Gly Thr Ser Met Ser Pro Gln 
            180                 185                 190         


Leu Cys Leu Asp Pro Met Asp Val Val Lys Leu Glu Pro Pro Met Leu 
        195                 200                 205             


Arg Thr Met Asp Pro Ser Pro Glu Ala Ala Pro Pro Gln Ala Gly Cys 
    210                 215                 220                 


Leu Gln Leu Ser Trp Glu Pro Trp Gln Pro Gly Leu His Ile Asn Gln 
225                 230                 235                 240 


Lys Cys Glu Leu Arg His Lys Pro Gln Arg Gly Glu Ala Ser Trp Ala 
                245                 250                 255     


Leu Val Gly Pro Leu Pro Leu Glu Ala Leu Gln Tyr Glu Leu Cys Gly 
            260                 265                 270         


Leu Leu Pro Ala Thr Ala Tyr Thr Leu Gln Ile Arg Cys Ile Arg Trp 
        275                 280                 285             


Pro Leu Pro Gly His Trp Ser Asp Trp Ser Pro Ser Leu Glu Leu Arg 
    290                 295                 300                 


Thr Thr Glu Arg Ala Pro Thr His His His His His His His His 
305                 310                 315                 


<210>  2
<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  VH of C1.2

<400>  2

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Met Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  3
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  VL of C1.2

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Ala Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Leu Gln Asn Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr His Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Asn Val Glu Ile Arg 
            100                 105         


<210>  4
<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  VH of C1.2G

<400>  4

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  5
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  VL  of C1.2G

<400>  5

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Leu Gln Asn Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  6
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  HCDR1 of C1.2

<400>  6

Leu Tyr Trp Met Gly 
1               5   


<210>  7
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 HCDR2

<400>  7

Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  8
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 HCDR3

<400>  8

Leu Gly Glu Leu Gly Trp Phe Asp Pro 
1               5                   


<210>  9
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 LCDR1

<400>  9

Arg Ala Ser Gln Gly Ile Ser Ser Tyr Leu Asn 
1               5                   10      


<210>  10
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 LCDR2

<400>  10

Ala Ser Asn Leu Gln Asn 
1               5       


<210>  11
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 LCDR3

<400>  11

Gln Gln Ser Tyr Ser Thr Pro Leu Thr 
1               5                   


<210>  12
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  consensus sequence of HCDR3 of C1.2


<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is an amino acid selected from the group consisting of 
       tryptophan, glutamine, methionine, serine, phenylalanine, 
       glutamic acid and histidine

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X iis an amino acid selected from the group consisting of 
       phenylalanine, tyrosine, methionine, serine, glycine and 
       isoleucine

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  X is an amino acid selected from the group consisting of aspartic
       acid, methionine, glutamine, serine, leucine, valine, arginine 
       and histidine

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  X is an amino acid selected from the group consisting of proline 
       gltuamic acid, alanine, leucine, phenylalanine, tyronis, 
       threonine, asparagine, aspartic acid, serine , glycine, arginine,
       lysine

<400>  12

Leu Gly Glu Leu Xaa Xaa Xaa Xaa 
1               5               


<210>  13
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  consensus sequence of LCDR3 of C1.2


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is an amino acid selected from the group consisting of 
       glutamine, glutamic acid, histidine, alanine or serine

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is an amino acid selected from the group consisting of 
       glutamine, valine, phenylalanine, asparagine and glutamic acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X ian amino acid selected from the group consisting of serine or 
       glycine

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is an amino acid selected from the group consisting of 
       tryptophan, methionine, phenylalanine, tyrosine, isoleucine and 
       leucine

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is an amino acid selected from the group consisting of glutamic
       acid, methionine, glutamine, tryptophan, serine, valine, 
       asparagine, glycine, alanine, arganine, histidine, tyrosine, 
       lysine or threonine

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is an amino acid selected from the group consisting of 
       tyrosine, methionine, isoleucine or threonine

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  X is an amino acid selected from the group consisting of proline,
       alanine, histidine, glycine and lysine

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  X is an amino acid selected from the group consisting of leucine,
       glutamine, methionine, alanine, phenylalanine, isoleucine, 
       lysine, histidine and glycine

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  X is an amino acid selected from the group consisting of 
       threonine, phenylalanine, tyrosine, methionine, lysine, serine, 
       histidine, proline, tryptophan, isoleucine, glutamine, glycine 
       and valine

<400>  13

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   


<210>  14
<211>  445
<212>  PRT
<213>  artificial

<220>
<223>  C1.2G heavy chain IgG4 with S241P mutation

<400>  14

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        435                 440                 445 


<210>  15
<211>  214
<212>  PRT
<213>  artificial

<220>
<223>  C1.2G with kappa light chain

<400>  15

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Leu Gln Asn Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  16
<211>  444
<212>  PRT
<213>  artificial

<220>
<223>  C1.2G heavy chain IgG4 with S241P mutation and lacking C-terminal
       lysine residue

<400>  16

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
        435                 440                 


