
                                SEQUENCE LISTING

<110> Henrich, Ian
      Chou, Margaret M.

<120> Compositions and Methods for Treating
  Cancer

<130> 3460-P06634WO00

<150> 62/771,869          
<151> 2018-11-27  

<150> 62/796,959          
<151> 2019-01-25  

<150> 62/893,492          
<151> 2019-08-29  

<160> 7

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1406
<212> PRT
<213> Homo sapiens

<400> 1
Met Asp Met Val Glu Asn Ala Asp Ser Leu Gln Ala Gln Glu Arg Lys
 1               5                  10                  15      
Asp Ile Leu Met Lys Tyr Asp Lys Gly His Arg Ala Gly Leu Pro Glu
            20                  25                  30          
Asp Lys Gly Pro Glu Pro Val Gly Ile Asn Ser Ser Ile Asp Arg Phe
        35                  40                  45              
Gly Ile Leu His Glu Thr Glu Leu Pro Pro Val Thr Ala Arg Glu Ala
    50                  55                  60                  
Lys Lys Ile Arg Arg Glu Met Thr Arg Thr Ser Lys Trp Met Glu Met
65                  70                  75                  80  
Leu Gly Glu Trp Glu Thr Tyr Lys His Ser Ser Lys Leu Ile Asp Arg
                85                  90                  95      
Val Tyr Lys Gly Ile Pro Met Asn Ile Arg Gly Pro Val Trp Ser Val
            100                 105                 110         
Leu Leu Asn Ile Gln Glu Ile Lys Leu Lys Asn Pro Gly Arg Tyr Gln
        115                 120                 125             
Ile Met Lys Glu Arg Gly Lys Arg Ser Ser Glu His Ile His His Ile
    130                 135                 140                 
Asp Leu Asp Val Arg Thr Thr Leu Arg Asn His Val Phe Phe Arg Asp
145                 150                 155                 160 
Arg Tyr Gly Ala Lys Gln Arg Glu Leu Phe Tyr Ile Leu Leu Ala Tyr
                165                 170                 175     
Ser Glu Tyr Asn Pro Glu Val Gly Tyr Cys Arg Asp Leu Ser His Ile
            180                 185                 190         
Thr Ala Leu Phe Leu Leu Tyr Leu Pro Glu Glu Asp Ala Phe Trp Ala
        195                 200                 205             
Leu Val Gln Leu Leu Ala Ser Glu Arg His Ser Leu Pro Gly Phe His
    210                 215                 220                 
Ser Pro Asn Gly Gly Thr Val Gln Gly Leu Gln Asp Gln Gln Glu His
225                 230                 235                 240 
Val Val Pro Lys Ser Gln Pro Lys Thr Met Trp His Gln Asp Lys Glu
                245                 250                 255     
Gly Leu Cys Gly Gln Cys Ala Ser Leu Gly Cys Leu Leu Arg Asn Leu
            260                 265                 270         
Ile Asp Gly Ile Ser Leu Gly Leu Thr Leu Arg Leu Trp Asp Val Tyr
        275                 280                 285             
Leu Val Glu Gly Glu Gln Val Leu Met Pro Ile Thr Ser Ile Ala Leu
    290                 295                 300                 
Lys Val Gln Gln Lys Arg Leu Met Lys Thr Ser Arg Cys Gly Leu Trp
305                 310                 315                 320 
Ala Arg Leu Arg Asn Gln Phe Phe Asp Thr Trp Ala Met Asn Asp Asp
                325                 330                 335     
Thr Val Leu Lys His Leu Arg Ala Ser Thr Lys Lys Leu Thr Arg Lys
            340                 345                 350         
Gln Gly Asp Leu Pro Pro Pro Ala Lys Arg Glu Gln Gly Ser Leu Ala
        355                 360                 365             
Pro Arg Pro Val Pro Ala Ser Arg Gly Gly Lys Thr Leu Cys Lys Gly
    370                 375                 380                 
Tyr Arg Gln Ala Pro Pro Gly Pro Pro Ala Gln Phe Gln Arg Pro Ile
385                 390                 395                 400 
Cys Ser Ala Ser Pro Pro Trp Ala Ser Arg Phe Ser Thr Pro Cys Pro
                405                 410                 415     
Gly Gly Ala Val Arg Glu Asp Thr Tyr Pro Val Gly Thr Gln Gly Val
            420                 425                 430         
Pro Ser Leu Ala Leu Ala Gln Gly Gly Pro Gln Gly Ser Trp Arg Phe
        435                 440                 445             
Leu Glu Trp Lys Ser Met Pro Arg Leu Pro Thr Asp Leu Asp Ile Gly
    450                 455                 460                 
Gly Pro Trp Phe Pro His Tyr Asp Phe Glu Trp Ser Cys Trp Val Arg
465                 470                 475                 480 
Ala Ile Ser Gln Glu Asp Gln Leu Ala Thr Cys Trp Gln Ala Glu His
                485                 490                 495     
Cys Gly Glu Val His Asn Lys Asp Met Ser Trp Pro Glu Glu Met Ser
            500                 505                 510         
Phe Thr Ala Asn Ser Ser Lys Ile Asp Arg Gln Lys Val Pro Thr Glu
        515                 520                 525             
Lys Gly Ala Thr Gly Leu Ser Asn Leu Gly Asn Thr Cys Phe Met Asn
    530                 535                 540                 
Ser Ser Ile Gln Cys Val Ser Asn Thr Gln Pro Leu Thr Gln Tyr Phe
545                 550                 555                 560 
Ile Ser Gly Arg His Leu Tyr Glu Leu Asn Arg Thr Asn Pro Ile Gly
                565                 570                 575     
Met Lys Gly His Met Ala Lys Cys Tyr Gly Asp Leu Val Gln Glu Leu
            580                 585                 590         
Trp Ser Gly Thr Gln Lys Ser Val Ala Pro Leu Lys Leu Arg Arg Thr
        595                 600                 605             
Ile Ala Lys Tyr Ala Pro Lys Phe Asp Gly Phe Gln Gln Gln Asp Ser
    610                 615                 620                 
Gln Glu Leu Leu Ala Phe Leu Leu Asp Gly Leu His Glu Asp Leu Asn
625                 630                 635                 640 
Arg Val His Glu Lys Pro Tyr Val Glu Leu Lys Asp Ser Asp Gly Arg
                645                 650                 655     
Pro Asp Trp Glu Val Ala Ala Glu Ala Trp Asp Asn His Leu Arg Arg
            660                 665                 670         
Asn Arg Ser Ile Ile Val Asp Leu Phe His Gly Gln Leu Arg Ser Gln
        675                 680                 685             
Val Lys Cys Lys Thr Cys Gly His Ile Ser Val Arg Phe Asp Pro Phe
    690                 695                 700                 
Asn Phe Leu Ser Leu Pro Leu Pro Met Asp Ser Tyr Met Asp Leu Glu
705                 710                 715                 720 
Ile Thr Val Ile Lys Leu Asp Gly Thr Thr Pro Val Arg Tyr Gly Leu
                725                 730                 735     
Arg Leu Asn Met Asp Glu Lys Tyr Thr Gly Leu Lys Lys Gln Leu Arg
            740                 745                 750         
Asp Leu Cys Gly Leu Asn Ser Glu Gln Ile Leu Leu Ala Glu Val His
        755                 760                 765             
Asp Ser Asn Ile Lys Asn Phe Pro Gln Asp Asn Gln Lys Val Gln Leu
    770                 775                 780                 
Ser Val Ser Gly Phe Leu Cys Ala Phe Glu Ile Pro Val Pro Ser Ser
785                 790                 795                 800 
Pro Ile Ser Ala Ser Ser Pro Thr Gln Ile Asp Phe Ser Ser Ser Pro
                805                 810                 815     
Ser Thr Asn Gly Met Phe Thr Leu Thr Thr Asn Gly Asp Leu Pro Lys
            820                 825                 830         
Pro Ile Phe Ile Pro Asn Gly Met Pro Asn Thr Val Val Pro Cys Gly
        835                 840                 845             
Thr Glu Lys Asn Phe Thr Asn Gly Met Val Asn Gly His Met Pro Ser
    850                 855                 860                 
Leu Pro Asp Ser Pro Phe Thr Gly Tyr Ile Ile Ala Val His Arg Lys
865                 870                 875                 880 
Met Met Arg Thr Glu Leu Tyr Phe Leu Ser Pro Gln Glu Asn Arg Pro
                885                 890                 895     
Ser Leu Phe Gly Met Pro Leu Ile Val Pro Cys Thr Val His Thr Arg
            900                 905                 910         
Lys Lys Asp Leu Tyr Asp Ala Val Trp Ile Gln Val Ser Trp Leu Ala
        915                 920                 925             
Arg Pro Leu Pro Pro Gln Glu Ala Ser Ile His Ala Gln Asp Arg Asp
    930                 935                 940                 
Asn Cys Met Gly Tyr Gln Tyr Pro Phe Thr Leu Arg Val Val Gln Lys
945                 950                 955                 960 
Asp Gly Asn Ser Cys Ala Trp Cys Pro Gln Tyr Arg Phe Cys Arg Gly
                965                 970                 975     
Cys Lys Ile Asp Cys Gly Glu Asp Arg Ala Phe Ile Gly Asn Ala Tyr
            980                 985                 990         
Ile Ala Val Asp Trp His Pro Thr Ala Leu His Leu Arg Tyr Gln Thr
        995                 1000                1005            
Ser Gln Glu Arg Val Val Asp Lys His Glu Ser Val Glu Gln Ser Arg
    1010                1015                1020                
Arg Ala Gln Ala Glu Pro Ile Asn Leu Asp Ser Cys Leu Arg Ala Phe
1025                1030                1035               1040 
Thr Ser Glu Glu Glu Leu Gly Glu Ser Glu Met Tyr Tyr Cys Ser Lys
                1045                1050                1055    
Cys Lys Thr His Cys Leu Ala Thr Lys Lys Leu Asp Leu Trp Arg Leu
            1060                1065                1070        
Pro Pro Phe Leu Ile Ile His Leu Lys Arg Phe Gln Phe Val Asn Asp
        1075                1080                1085            
Gln Trp Ile Lys Ser Gln Lys Ile Val Arg Phe Leu Arg Glu Ser Phe
    1090                1095                1100                
Asp Pro Ser Ala Phe Leu Val Pro Arg Asp Pro Ala Leu Cys Gln His
1105                1110                1115               1120 
Lys Pro Leu Thr Pro Gln Gly Asp Glu Leu Ser Lys Pro Arg Ile Leu
                1125                1130                1135    
Ala Arg Glu Val Lys Lys Val Asp Ala Gln Ser Ser Ala Gly Lys Glu
            1140                1145                1150        
Asp Met Leu Leu Ser Lys Ser Pro Ser Ser Leu Ser Ala Asn Ile Ser
        1155                1160                1165            
Ser Ser Pro Lys Gly Ser Pro Ser Ser Ser Arg Lys Ser Gly Thr Ser
    1170                1175                1180                
Cys Pro Ser Ser Lys Asn Ser Ser Pro Asn Ser Ser Pro Arg Thr Leu
1185                1190                1195               1200 
Gly Arg Ser Lys Gly Arg Leu Arg Leu Pro Gln Ile Gly Ser Lys Asn
                1205                1210                1215    
Lys Pro Ser Ser Ser Lys Lys Asn Leu Asp Ala Ser Lys Glu Asn Gly
            1220                1225                1230        
Ala Gly Gln Ile Cys Glu Leu Ala Asp Ala Leu Ser Arg Gly His Met
        1235                1240                1245            
Arg Gly Gly Ser Gln Pro Glu Leu Val Thr Pro Gln Asp His Glu Val
    1250                1255                1260                
Ala Leu Ala Asn Gly Phe Leu Tyr Glu His Glu Ala Cys Gly Asn Gly
1265                1270                1275               1280 
Cys Gly Asp Gly Tyr Ser Asn Gly Gln Leu Gly Asn His Ser Glu Glu
                1285                1290                1295    
Asp Ser Thr Asp Asp Gln Arg Glu Asp Thr His Ile Lys Pro Ile Tyr
            1300                1305                1310        
Asn Leu Tyr Ala Ile Ser Cys His Ser Gly Ile Leu Ser Gly Gly His
        1315                1320                1325            
Tyr Ile Thr Tyr Ala Lys Asn Pro Asn Cys Lys Trp Tyr Cys Tyr Asn
    1330                1335                1340                
Asp Ser Ser Cys Glu Glu Leu His Pro Asp Glu Ile Asp Thr Asp Ser
1345                1350                1355               1360 
Ala Tyr Ile Leu Phe Tyr Glu Gln Gln Gly Ile Asp Tyr Ala Gln Phe
                1365                1370                1375    
Leu Pro Lys Ile Asp Gly Lys Lys Met Ala Asp Thr Ser Ser Thr Asp
            1380                1385                1390        
Glu Asp Ser Glu Ser Asp Tyr Glu Lys Tyr Ser Met Leu Gln
        1395                1400                1405    


<210> 2
<211> 4221
<212> DNA
<213> Homo sapiens

<400> 2
atggacatgg tagagaatgc agatagtttg caggcacagg agcggaagga catacttatg 60
aagtatgaca agggacaccg agctgggctg ccagaggaca aggggcctga gcccgttgga 120
atcaacagca gcattgatcg ttttggcatt ttgcatgaga cggagctgcc tcctgtgact 180
gcacgggagg cgaagaaaat tcggcgggag atgacacgaa cgagcaagtg gatggaaatg 240
ctgggagaat gggagacata taagcacagt agcaaactca tagatcgagt gtacaaggga 300
attcccatga acatccgggg cccggtgtgg tcagtcctcc tgaacattca ggaaatcaag 360
ttgaaaaacc ccggaagata ccagatcatg aaggagaggg gcaagaggtc atctgaacac 420
atccaccaca tcgacctgga cgtgaggacg actctccgga accatgtctt ctttagggat 480
cgatatggag ccaagcagag ggaactattc tacatcctcc tggcctattc ggagtataac 540
ccggaggtgg gctactgcag ggacctgagc cacatcaccg ccttgttcct cctttatctg 600
cctgaggagg acgcattctg ggcactggtg cagctgctgg ccagtgagag gcactccctg 660
ccaggattcc acagcccaaa tggtgggaca gtccaggggc tccaagacca acaggagcat 720
gtggtaccca agtcacaacc caagaccatg tggcatcagg acaaggaagg tctatgcggg 780
cagtgtgcct cgttaggctg ccttctccgg aacctgattg acgggatctc tctcgggctc 840
accctgcgcc tgtgggacgt gtatttggtg gaaggagaac aggtgttgat gccaataacc 900
agcattgctc ttaaggttca gcagaagcgc ctcatgaaga catccaggtg tggcctgtgg 960
gcacgtctgc ggaaccaatt cttcgatacc tgggccatga acgatgacac cgtgctcaag 1020
catcttaggg cctctacgaa gaaactaaca aggaagcaag gggacctgcc acccccagcc 1080
aaacgcgagc aagggtcctt ggcacccagg cctgtgccgg cttcacgtgg tgggaagacc 1140
ctctgcaagg ggtataggca ggcccctcca ggcccaccag cccagttcca gcggcccatt 1200
tgctcagctt ccccgccatg ggcatctcgt ttttccacgc cctgtcctgg tggggctgtc 1260
cgggaagaca cgtaccctgt gggcactcag ggtgtgccca gcctggccct ggctcaggga 1320
ggacctcagg gttcctggag attcctggag tggaagtcaa tgccccggct cccaacggac 1380
ctggatatag ggggcccttg gttcccccat tatgattttg aatggagctg ctgggtccgt 1440
gccatatccc aggaggacca gctggccacc tgctggcagg ctgaacactg cggagaggtt 1500
cacaacaaag atatgagttg gcctgaggag atgtctttta cagcaaatag tagtaaaata 1560
gatagacaaa aggttcccac agaaaaggga gccacaggtc taagcaacct gggaaacaca 1620
tgcttcatga actcaagcat ccagtgcgtt agtaacacac agccactgac acagtatttt 1680
atctcaggga gacatcttta tgaactcaac aggacaaatc ccattggtat gaaggggcat 1740
atggctaaat gctatggtga tttagtgcag gaactctgga gtggaactca gaagagtgtt 1800
gccccattaa agcttcggcg gaccatagca aaatatgctc ccaagtttga tgggtttcag 1860
caacaagact cccaagaact tctggctttt ctcttggatg gtcttcatga agatctcaac 1920
cgagtccatg aaaagccata tgtggaactg aaggacagtg atggccgacc agactgggaa 1980
gtagctgcag aggcctggga caaccatcta agaagaaata gatcaattat tgtggatttg 2040
ttccatgggc agctaagatc tcaagtcaaa tgcaagacat gtgggcatat aagtgtccga 2100
tttgaccctt tcaatttttt gtctttgcca ctaccaatgg acagttacat ggacttagaa 2160
ataacagtga ttaagttaga tggtactacc cctgtacggt atggactaag actgaatatg 2220
gatgaaaagt acacaggttt aaaaaaacag ctgagggatc tctgtggact taattcagaa 2280
caaatcctac tagcagaagt acatgattcc aacataaaga actttcctca ggataaccaa 2340
aaagtacaac tctcagtgag cggatttttg tgtgcatttg aaattcctgt cccttcatct 2400
ccaatttcag cttctagtcc aacacaaata gatttctcct cttcaccatc tacaaatgga 2460
atgttcaccc taactaccaa tggggaccta cccaaaccaa tattcatccc caatggaatg 2520
ccaaacactg ttgtgccatg tggaactgag aagaacttca caaatggaat ggttaatggt 2580
cacatgccat ctcttcctga cagccccttt acaggttaca tcattgcagt ccaccgaaaa 2640
atgatgagga cagaactgta tttcctgtca cctcaggaga atcgccccag cctctttgga 2700
atgccattga ttgttccatg cactgtgcat acccggaaga aagacctata tgatgcggtt 2760
tggattcaag tatcctggtt agcaagacca ctcccacctc aggaagctag tattcatgcc 2820
caggatcgtg ataactgtat gggctatcaa tatccattca ctctacgagt tgtgcagaaa 2880
gatgggaact cctgtgcttg gtgcccacag tatagatttt gcagaggctg taaaattgat 2940
tgtggggaag acagagcttt cattggaaat gcctatattg ctgtggattg gcaccccaca 3000
gcccttcacc ttcgctatca aacatcccag gaaagggttg tagataagca tgagagtgtg 3060
gagcagagtc ggcgagcgca agccgagccc atcaacctgg acagctgtct ccgtgctttc 3120
accagtgagg aagagctagg ggaaagtgag atgtactact gttccaagtg taagacccac 3180
tgcttagcaa caaagaagct ggatctctgg aggcttccac ccttcctgat tattcacctt 3240
aagcgatttc aatttgtaaa tgatcagtgg ataaaatcac agaaaattgt cagatttctt 3300
cgggaaagtt ttgatccgag tgcttttttg gtaccacgag acccggccct ctgccagcat 3360
aaaccactca caccccaggg ggatgagctc tccaagccca ggattctggc aagagaggtg 3420
aagaaagtgg atgcgcagag ttcggctgga aaagaggaca tgctcctaag caaaagccca 3480
tcctcactca gcgctaacat cagcagcagc ccaaaaggtt ctccttcttc atcaagaaaa 3540
agtggaacca gctgtccctc cagcaaaaac agcagcccta atagcagccc acggactttg 3600
gggaggagca aagggaggct ccggctgccc cagattggca gcaaaaataa gccgtcaagt 3660
agtaagaaga acttggatgc cagcaaagag aatggggctg ggcagatctg tgagctggct 3720
gacgccttga gccgagggca tatgcggggg ggcagccaac cagagctggt cactcctcag 3780
gaccatgagg tagctttggc caatggattc ctttatgagc atgaagcatg tggcaatggc 3840
tgtggcgatg gctacagcaa tggtcagctt ggaaaccaca gtgaagaaga cagcactgat 3900
gaccaaagag aagacactca tattaagcct atttataatc tatatgcaat ttcatgccat 3960
tcaggaattc tgagtggggg ccattacatc acttatgcca aaaacccaaa ctgcaagtgg 4020
tactgttata atgacagcag ctgtgaggaa cttcaccctg atgaaattga caccgactct 4080
gcctacattc ttttctatga gcagcagggg atagactacg cacaatttct gccaaagatt 4140
gatggcaaaa agatggcaga cacaagcagt acggatgaag actctgagtc tgattacgaa 4200
aagtactcta tgttacagta a                                           4221

<210> 3
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Jak1 gRNA target

<400> 3
caccgtccca tacctcatcc ggtag                                       25

<210> 4
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> STAT1 gRNA target

<400> 4
caccgtccca ttacaggctc agtcg                                       25

<210> 5
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> STAT3 gRNA target

<400> 5
caccgagatt gcccggattg tggcc                                       25

<210> 6
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic Sequence

<400> 6
tttttttttt t                                                      11

<210> 7
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> poly(A) tail segment

<400> 7
aaaaaaaaaa aaa                                                    13
