                         SEQUENCE LISTING

<110>  Casebia Therapeutics LLP
       Cheng, Christopher J.
       Scharenberg, Andrew
       Wang, Kui
       Sane, Shailendra
 
<120>  Optimized mRNA Encoding CAS9 for Use in LNPs

<130>  052984-536001WO

<150>  US 62/772,278
<151>  2018-11-28

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  1057
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  Gib11SpaCas9-1 polypeptide

<400>  1

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn Lys Glu 
65                  70                  75                  80  


Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu 
        115                 120                 125             


Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn 
    130                 135                 140                 


Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg 
145                 150                 155                 160 


Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr 
                165                 170                 175     


Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln 
            180                 185                 190         


Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu 
        195                 200                 205             


Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro 
    210                 215                 220                 


Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly 
225                 230                 235                 240 


His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr 
                245                 250                 255     


Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile 
            260                 265                 270         


Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile 
        275                 280                 285             


Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile 
    290                 295                 300                 


Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile 
305                 310                 315                 320 


Thr Lys Ser Gly Thr Pro Glu Phe Thr Ser Phe Lys Leu Phe His Asp 
                325                 330                 335     


Leu Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu 
            340                 345                 350         


Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser 
        355                 360                 365             


Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp 
    370                 375                 380                 


Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu 
385                 390                 395                 400 


Ser Leu Lys Cys Met Asn Met Ile Ile Asp Glu Leu Trp His Ser Ser 
                405                 410                 415     


Met Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro Lys Lys 
            420                 425                 430         


Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp 
        435                 440                 445             


Ala Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn 
    450                 455                 460                 


Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile 
465                 470                 475                 480 


Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile 
                485                 490                 495     


Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu 
            500                 505                 510         


Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys 
        515                 520                 525             


Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu 
    530                 535                 540                 


Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val 
545                 550                 555                 560 


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn 
                565                 570                 575     


Lys Val Leu Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr 
            580                 585                 590         


Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln 
        595                 600                 605             


Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser 
    610                 615                 620                 


Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe 
625                 630                 635                 640 


Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr 
                645                 650                 655     


Ala Thr Arg Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn 
            660                 665                 670         


Asn Met Asn Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr 
        675                 680                 685             


Leu Arg Lys Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys 
    690                 695                 700                 


His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe 
705                 710                 715                 720 


Lys Glu Asn Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro 
                725                 730                 735     


Glu Ile Glu Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn 
            740                 745                 750         


Tyr Ser Glu Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp 
        755                 760                 765             


Phe Arg Asn Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg 
    770                 775                 780                 


Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser 
785                 790                 795                 800 


Thr Tyr Ile Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr 
                805                 810                 815     


Thr Leu Lys Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr 
            820                 825                 830         


Gln His Asp Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln 
        835                 840                 845             


Tyr Ala Asn Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly 
    850                 855                 860                 


Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys 
865                 870                 875                 880 


Ser Leu Lys Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr 
                885                 890                 895     


His Gln Phe Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys 
            900                 905                 910         


Asn Tyr Arg Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys Phe Val 
        915                 920                 925             


Thr Ile Ala Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile 
    930                 935                 940                 


Pro Lys Asp Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys Ile Lys Asp 
945                 950                 955                 960 


Thr Asp Gln Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu 
                965                 970                 975     


Asn Gly Asp Leu Tyr Lys Ile Ile Gly Val Asn Ser Asp Asp Arg Asn 
            980                 985                 990         


Ile Ile Glu Leu Asp Tyr Tyr Asp  Ile Lys Tyr Lys Asp  Tyr Cys Glu 
        995                 1000                 1005             


Ile Asn  Asn Ile Lys Gly Glu  Pro Arg Ile Lys Lys  Thr Ile Gly 
    1010                 1015                 1020             


Lys Lys  Thr Glu Ser Ile Glu  Lys Phe Thr Thr Asp  Val Leu Gly 
    1025                 1030                 1035             


Asn Leu  Tyr Leu His Ser Thr  Glu Lys Ala Pro Gln  Leu Ile Phe 
    1040                 1045                 1050             


Lys Arg  Gly Leu 
    1055         


<210>  2
<211>  1056
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  Gib11SpaCas9-3 polypeptide

<400>  2

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn Lys Glu 
65                  70                  75                  80  


Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu 
        115                 120                 125             


Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn 
    130                 135                 140                 


Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg 
145                 150                 155                 160 


Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr 
                165                 170                 175     


Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln 
            180                 185                 190         


Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu 
        195                 200                 205             


Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro 
    210                 215                 220                 


Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly 
225                 230                 235                 240 


His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr 
                245                 250                 255     


Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile 
            260                 265                 270         


Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile 
        275                 280                 285             


Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile 
    290                 295                 300                 


Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile 
305                 310                 315                 320 


Thr Lys Ser Gly Thr Pro Glu Phe Thr Ser Phe Lys Leu Phe His Asp 
                325                 330                 335     


Leu Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu 
            340                 345                 350         


Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser 
        355                 360                 365             


Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp 
    370                 375                 380                 


Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu 
385                 390                 395                 400 


Ser Leu Lys Cys Met Asn Met Ile Ile Asp Glu Leu Trp His Ser Ser 
                405                 410                 415     


Met Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro Lys Lys 
            420                 425                 430         


Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp 
        435                 440                 445             


Ala Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn 
    450                 455                 460                 


Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile 
465                 470                 475                 480 


Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile 
                485                 490                 495     


Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu 
            500                 505                 510         


Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys 
        515                 520                 525             


Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu 
    530                 535                 540                 


Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val 
545                 550                 555                 560 


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn 
                565                 570                 575     


Lys Val Leu Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr 
            580                 585                 590         


Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln 
        595                 600                 605             


Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser 
    610                 615                 620                 


Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe 
625                 630                 635                 640 


Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr 
                645                 650                 655     


Ala Thr Arg Glu Leu Thr Ser Tyr Leu Lys Ala Tyr Phe Ser Ala Asn 
            660                 665                 670         


Asn Met Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn His 
        675                 680                 685             


Leu Arg Lys Val Trp Arg Phe Asp Lys Tyr Arg Asn His Gly Tyr Lys 
    690                 695                 700                 


His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe 
705                 710                 715                 720 


Lys Glu Asn Lys Lys Leu Gln Asn Thr Asn Lys Ile Leu Glu Lys Pro 
                725                 730                 735     


Thr Ile Glu Asn Asn Thr Lys Lys Val Thr Val Glu Lys Glu Glu Asp 
            740                 745                 750         


Tyr Asn Asn Val Phe Glu Thr Pro Lys Leu Val Glu Asp Ile Lys Gln 
        755                 760                 765             


Tyr Arg Asp Tyr Lys Phe Ser His Arg Val Asp Lys Lys Pro Asn Arg 
    770                 775                 780                 


Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Met Lys Asp Glu His 
785                 790                 795                 800 


Asp Tyr Ile Val Gln Thr Ile Thr Asp Ile Tyr Gly Lys Asp Asn Thr 
                805                 810                 815     


Asn Leu Lys Lys Gln Phe Asn Lys Asn Pro Glu Lys Phe Leu Met Tyr 
            820                 825                 830         


Gln Asn Asp Pro Lys Thr Phe Glu Lys Leu Ser Ile Ile Met Lys Gln 
        835                 840                 845             


Tyr Ser Asp Glu Lys Asn Pro Leu Ala Lys Tyr Tyr Glu Glu Thr Gly 
    850                 855                 860                 


Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys 
865                 870                 875                 880 


Lys Ile Lys Leu Leu Gly Asn Lys Val Gly Asn His Leu Asp Val Thr 
                885                 890                 895     


Asn Lys Tyr Glu Asn Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys 
            900                 905                 910         


Asn Tyr Arg Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys Phe Val 
        915                 920                 925             


Thr Ile Ala Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile 
    930                 935                 940                 


Pro Lys Asp Lys Tyr Glu Leu Lys Glu Lys Lys Lys Ile Lys Asp Thr 
945                 950                 955                 960 


Asp Gln Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asn 
                965                 970                 975     


Gly Asp Leu Tyr Lys Ile Ile Gly Val Asn Ser Asp Asp Arg Asn Ile 
            980                 985                 990         


Ile Glu Leu Asp Tyr Tyr Asp Ile  Lys Tyr Lys Asp Tyr  Cys Glu Ile 
        995                 1000                 1005             


Asn Asn  Ile Lys Gly Glu Pro  Arg Ile Lys Lys Thr  Ile Gly Lys 
    1010                 1015                 1020             


Lys Thr  Glu Ser Ile Glu Lys  Phe Thr Thr Asp Val  Leu Gly Asn 
    1025                 1030                 1035             


Leu Tyr  Leu His Ser Thr Glu  Lys Ala Pro Gln Leu  Ile Phe Lys 
    1040                 1045                 1050             


Arg Gly  Leu 
    1055     


<210>  3
<211>  1057
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  E2Cas9 polypeptide

<400>  3

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Asp Arg Val Lys His Leu Leu Ala Glu Tyr Asp Leu Leu Asp Leu Thr 
65                  70                  75                  80  


Asn Ile Pro Lys Ser Thr Asn Pro Tyr Gln Thr Arg Val Lys Gly Leu 
                85                  90                  95      


Asn Glu Lys Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp Lys Glu 
        115                 120                 125             


Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn Lys Asn 
    130                 135                 140                 


Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys Glu Arg 
145                 150                 155                 160 


Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe Leu Thr 
                165                 170                 175     


Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln Met Gln 
            180                 185                 190         


Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile Ser Leu 
        195                 200                 205             


Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro 
    210                 215                 220                 


Phe Gly Trp Glu Gly Asn Ile Lys Lys Trp Phe Glu Gln Met Met Gly 
225                 230                 235                 240 


His Cys Thr Tyr Phe Pro Glu Glu Leu Arg Ser Val Lys Tyr Ser Tyr 
                245                 250                 255     


Ser Ala Glu Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile 
            260                 265                 270         


Thr Arg Asp Glu Asp Ala Lys Leu Asn Tyr Gly Glu Lys Phe Gln Ile 
        275                 280                 285             


Ile Glu Asn Val Phe Lys Gln Lys Lys Thr Pro Asn Leu Lys Gln Ile 
    290                 295                 300                 


Ala Ile Glu Ile Gly Val His Glu Thr Glu Ile Lys Gly Tyr Arg Val 
305                 310                 315                 320 


Asn Lys Ser Gly Thr Pro Glu Phe Thr Glu Phe Lys Leu Tyr His Asp 
                325                 330                 335     


Leu Lys Ser Ile Val Phe Asp Lys Ser Ile Leu Glu Asn Glu Ala Ile 
            340                 345                 350         


Leu Asp Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Glu Gln Ser 
        355                 360                 365             


Ile Lys Glu Glu Leu Asn Lys Leu Pro Glu Ile Leu Asn Glu Gln Asp 
    370                 375                 380                 


Lys Ala Glu Ile Ala Lys Leu Ile Gly Tyr Asn Gly Thr His Arg Leu 
385                 390                 395                 400 


Ser Leu Lys Cys Ile His Leu Ile Asn Glu Glu Leu Trp Gln Thr Ser 
                405                 410                 415     


Arg Asn Gln Met Glu Ile Phe Asn Tyr Leu Asn Ile Lys Pro Asn Lys 
            420                 425                 430         


Val Asp Leu Ser Glu Gln Asn Lys Ile Pro Lys Asp Met Val Asn Asp 
        435                 440                 445             


Phe Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn 
    450                 455                 460                 


Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile 
465                 470                 475                 480 


Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile 
                485                 490                 495     


Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu 
            500                 505                 510         


Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys 
        515                 520                 525             


Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Lys 
    530                 535                 540                 


Asp Ile Pro Leu Glu Asp Leu Leu Arg Asn Pro Asn Asn Tyr Asp Ile 
545                 550                 555                 560 


Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asp Ser Met His Asn 
                565                 570                 575     


Lys Val Leu Val Arg Arg Glu Gln Asn Ala Lys Lys Asn Asn Gln Thr 
            580                 585                 590         


Pro Tyr Gln Tyr Leu Thr Ser Gly Tyr Ala Asp Ile Lys Tyr Ser Val 
        595                 600                 605             


Phe Lys Gln His Val Leu Asn Leu Ala Glu Asn Lys Asp Arg Met Thr 
    610                 615                 620                 


Lys Lys Lys Arg Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe 
625                 630                 635                 640 


Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr 
                645                 650                 655     


Ala Thr Arg Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn 
            660                 665                 670         


Asn Met Asn Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr 
        675                 680                 685             


Leu Arg Lys Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys 
    690                 695                 700                 


His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe 
705                 710                 715                 720 


Lys Glu Asn Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro 
                725                 730                 735     


Glu Ile Glu Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn 
            740                 745                 750         


Tyr Ser Glu Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp 
        755                 760                 765             


Phe Arg Asn Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg 
    770                 775                 780                 


Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser 
785                 790                 795                 800 


Thr Tyr Ile Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr 
                805                 810                 815     


Thr Leu Lys Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr 
            820                 825                 830         


Gln His Asp Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln 
        835                 840                 845             


Tyr Ala Asn Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly 
    850                 855                 860                 


Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys 
865                 870                 875                 880 


Ser Leu Lys Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr 
                885                 890                 895     


His Gln Phe Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys 
            900                 905                 910         


Pro Tyr Arg Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile 
        915                 920                 925             


Thr Ile Ser Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile 
    930                 935                 940                 


Pro Glu Gln Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys 
945                 950                 955                 960 


Asn Ala Lys Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu 
                965                 970                 975     


Asp Gly Glu Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn 
            980                 985                 990         


Met Ile Glu Leu Asp Leu Pro Asp  Ile Arg Tyr Lys Glu  Tyr Cys Glu 
        995                 1000                 1005             


Leu Asn  Asn Ile Lys Gly Glu  Pro Arg Ile Lys Lys  Thr Ile Gly 
    1010                 1015                 1020             


Lys Lys  Val Asn Ser Ile Glu  Lys Leu Thr Thr Asp  Val Leu Gly 
    1025                 1030                 1035             


Asn Val  Phe Thr Asn Thr Gln  Tyr Thr Lys Pro Gln  Leu Leu Phe 
    1040                 1045                 1050             


Lys Arg  Gly Asn 
    1055         


<210>  4
<211>  1053
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  F8Cas9 polypeptide

<400>  4

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Ser Leu Leu Ser Glu Tyr Lys Ile Ile Ser Gly Leu 
65                  70                  75                  80  


Ala Pro Thr Asn Asn Gln Pro Tyr Asn Ile Arg Val Lys Gly Leu Thr 
                85                  90                  95      


Glu Gln Leu Thr Lys Asp Glu Leu Ala Val Ala Leu Leu His Ile Ala 
            100                 105                 110         


Lys Arg Arg Gly Ile His Lys Ile Asp Val Ile Asp Ser Asn Asp Asp 
        115                 120                 125             


Val Gly Asn Glu Leu Ser Thr Lys Glu Gln Leu Asn Lys Asn Ser Lys 
    130                 135                 140                 


Leu Leu Lys Asp Lys Phe Val Cys Gln Ile Gln Leu Glu Arg Met Asn 
145                 150                 155                 160 


Glu Gly Gln Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Ala Asp Ile 
                165                 170                 175     


Ile Lys Glu Ile Ile Gln Leu Leu Asn Val Gln Lys Asn Phe His Gln 
            180                 185                 190         


Leu Asp Glu Asn Phe Ile Asn Lys Tyr Ile Glu Leu Val Glu Met Arg 
        195                 200                 205             


Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly Ser Pro Phe Gly Trp Asn 
    210                 215                 220                 


Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr 
225                 230                 235                 240 


Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp Leu 
                245                 250                 255     


Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Ile Ile Gln Arg Asp Asn 
            260                 265                 270         


Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn Val 
        275                 280                 285             


Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile 
    290                 295                 300                 


Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser Gly 
305                 310                 315                 320 


Thr Pro Glu Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser Val 
                325                 330                 335     


Leu Phe Asp Gln Ser Ile Leu Glu Asn Glu Asp Val Leu Asp Gln Ile 
            340                 345                 350         


Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Lys Ser Lys 
        355                 360                 365             


Leu Thr Glu Leu Asp Ile Leu Leu Asn Glu Glu Asp Lys Glu Asn Ile 
    370                 375                 380                 


Ala Gln Leu Thr Gly Tyr Asn Gly Thr His Arg Leu Ser Leu Lys Cys 
385                 390                 395                 400 


Ile Arg Leu Val Leu Glu Glu Gln Trp Tyr Ser Ser Arg Asn Gln Met 
                405                 410                 415     


Glu Ile Phe Thr His Leu Asn Ile Lys Pro Lys Lys Ile Asn Leu Thr 
            420                 425                 430         


Ala Ala Asn Lys Ile Pro Lys Ala Met Ile Asp Glu Phe Ile Leu Ser 
        435                 440                 445             


Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn Lys 
    450                 455                 460                 


Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu Ala 
465                 470                 475                 480 


Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu Gln 
                485                 490                 495     


Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly Gln 
            500                 505                 510         


Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu His 
        515                 520                 525             


Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Ala Leu 
    530                 535                 540                 


Met Asp Leu Leu Asn Asn Pro Gln Asn Tyr Glu Val Asp His Ile Ile 
545                 550                 555                 560 


Pro Arg Ser Val Ala Phe Asp Asn Ser Ile His Asn Lys Val Leu Val 
                565                 570                 575     


Lys Gln Ile Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Tyr Gln Tyr 
            580                 585                 590         


Leu Asn Ser Ser Asp Ala Lys Leu Ser Tyr Asn Gln Phe Lys Gln His 
        595                 600                 605             


Ile Leu Asn Leu Ser Lys Ser Lys Asp Arg Ile Ser Lys Lys Lys Lys 
    610                 615                 620                 


Asp Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln Lys 
625                 630                 635                 640 


Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu 
                645                 650                 655     


Leu Thr Ser Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asp Val 
            660                 665                 670         


Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asn His Leu Arg Lys Val 
        675                 680                 685             


Trp Arg Phe Asp Lys Tyr Arg Asn His Gly Tyr Lys His His Ala Glu 
    690                 695                 700                 


Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn Lys 
705                 710                 715                 720 


Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu Thr 
                725                 730                 735     


Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu Met 
            740                 745                 750         


Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn Phe 
        755                 760                 765             


Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn 
    770                 775                 780                 


Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile Val 
785                 790                 795                 800 


Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys Lys 
                805                 810                 815     


Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp Pro 
            820                 825                 830         


Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn Glu 
        835                 840                 845             


Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu Thr 
    850                 855                 860                 


Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys Tyr 
865                 870                 875                 880 


Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe Lys 
                885                 890                 895     


Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg Phe 
            900                 905                 910         


Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser Tyr 
        915                 920                 925             


Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln Lys 
    930                 935                 940                 


Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys Phe 
945                 950                 955                 960 


Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu Ile 
                965                 970                 975     


Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu Leu 
            980                 985                 990         


Asp Leu Pro Asp Ile Arg Tyr Lys  Glu Tyr Cys Glu Leu  Asn Asn Ile 
        995                 1000                 1005             


Lys Gly  Glu Pro Arg Ile Lys  Lys Thr Ile Gly Lys  Lys Val Asn 
    1010                 1015                 1020             


Ser Ile  Glu Lys Leu Thr Thr  Asp Val Leu Gly Asn  Val Phe Thr 
    1025                 1030                 1035             


Asn Thr  Gln Tyr Thr Lys Pro  Gln Leu Leu Phe Lys  Arg Gly Asn 
    1040                 1045                 1050             


<210>  5
<211>  1054
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  P2H12Cas9 polypeptide

<400>  5

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Lys Leu Leu Glu Asp Tyr Asn Leu Leu Asp Gln Ser 
65                  70                  75                  80  


Gln Ile Pro Gln Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ala Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Asn Ile Asn Val Ser Ser Glu Asp Glu 
        115                 120                 125             


Asp Ala Ser Asn Glu Leu Ser Thr Lys Glu Gln Ile Asn Arg Asn Asn 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Tyr Val Cys Glu Val Gln Leu Gln Arg Leu 
145                 150                 155                 160 


Lys Glu Gly Gln Ile Arg Gly Glu Lys Asn Arg Phe Lys Thr Thr Asp 
                165                 170                 175     


Ile Leu Lys Glu Ile Asp Gln Leu Leu Lys Val Gln Lys Asp Tyr His 
            180                 185                 190         


Asn Leu Asp Ile Asp Phe Ile Asn Gln Tyr Lys Glu Ile Val Glu Thr 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Tyr Gly Trp 
    210                 215                 220                 


Glu Gly Asp Pro Lys Ala Trp Tyr Glu Thr Leu Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Asp Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Gln Arg Asp 
            260                 265                 270         


Gly Leu Ser Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Asn Glu 
    290                 295                 300                 


Ile Asn Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Glu Phe Thr Ser Phe Lys Leu Phe His Asp Leu Lys Lys 
                325                 330                 335     


Val Val Lys Asp His Ala Ile Leu Asp Asp Ile Asp Leu Leu Asn Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Val Ala 
        355                 360                 365             


Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu Ala Asp Lys Gln Ser 
    370                 375                 380                 


Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His Ser Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Met Asn Met Ile Ile Asp Glu Leu Trp His Ser Ser Met Asn Gln 
                405                 410                 415     


Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro Lys Lys Tyr Glu Leu 
            420                 425                 430         


Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile Asp Asp Ala Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn 
    450                 455                 460                 


Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu 
                485                 490                 495     


Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu 
                725                 730                 735     


Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu 
            740                 745                 750         


Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn 
        755                 760                 765             


Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys 
                805                 810                 815     


Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp 
            820                 825                 830         


Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys 
865                 870                 875                 880 


Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe 
                885                 890                 895     


Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser 
        915                 920                 925             


Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln 
    930                 935                 940                 


Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu 
                965                 970                 975     


Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu 
            980                 985                 990         


Leu Asp Leu Pro Asp Ile Arg Tyr  Lys Glu Tyr Cys Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Val 
    1010                 1015                 1020             


Asn Ser  Ile Glu Lys Leu Thr  Thr Asp Val Leu Gly  Asn Val Phe 
    1025                 1030                 1035             


Thr Asn  Thr Gln Tyr Thr Lys  Pro Gln Leu Leu Phe  Lys Arg Gly 
    1040                 1045                 1050             


Asn 
    


<210>  6
<211>  1054
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  SluCas9 polypeptide

<400>  6

Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His Arg Leu 
    50                  55                  60                  


Glu Arg Val Lys Lys Leu Leu Glu Asp Tyr Asn Leu Leu Asp Gln Ser 
65                  70                  75                  80  


Gln Ile Pro Gln Ser Thr Asn Pro Tyr Ala Ile Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Glu Ala Leu Ser Lys Asp Glu Leu Val Ile Ala Leu Leu His Ile 
            100                 105                 110         


Ala Lys Arg Arg Gly Ile His Lys Ile Asp Val Ile Asp Ser Asn Asp 
        115                 120                 125             


Asp Val Gly Asn Glu Leu Ser Thr Lys Glu Gln Leu Asn Lys Asn Ser 
    130                 135                 140                 


Lys Leu Leu Lys Asp Lys Phe Val Cys Gln Ile Gln Leu Glu Arg Met 
145                 150                 155                 160 


Asn Glu Gly Gln Val Arg Gly Glu Lys Asn Arg Phe Lys Thr Ala Asp 
                165                 170                 175     


Ile Ile Lys Glu Ile Ile Gln Leu Leu Asn Val Gln Lys Asn Phe His 
            180                 185                 190         


Gln Leu Asp Glu Asn Phe Ile Asn Lys Tyr Ile Glu Leu Val Glu Met 
        195                 200                 205             


Arg Arg Glu Tyr Phe Glu Gly Pro Gly Lys Gly Ser Pro Tyr Gly Trp 
    210                 215                 220                 


Glu Gly Asp Pro Lys Ala Trp Tyr Glu Thr Leu Met Gly His Cys Thr 
225                 230                 235                 240 


Tyr Phe Pro Asp Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp 
                245                 250                 255     


Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Gln Arg Asp 
            260                 265                 270         


Gly Leu Ser Lys Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn 
        275                 280                 285             


Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Asn Glu 
    290                 295                 300                 


Ile Asn Val Asn Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser 
305                 310                 315                 320 


Gly Lys Pro Gln Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser 
                325                 330                 335     


Val Leu Phe Asp Gln Ser Ile Leu Glu Asn Glu Asp Val Leu Asp Gln 
            340                 345                 350         


Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Lys Ser 
        355                 360                 365             


Lys Leu Thr Glu Leu Asp Ile Leu Leu Asn Glu Glu Asp Lys Glu Asn 
    370                 375                 380                 


Ile Ala Gln Leu Thr Gly Tyr Thr Gly Thr His Arg Leu Ser Leu Lys 
385                 390                 395                 400 


Cys Ile Arg Leu Val Leu Glu Glu Gln Trp Tyr Ser Ser Arg Asn Gln 
                405                 410                 415     


Met Glu Ile Phe Thr His Leu Asn Ile Lys Pro Lys Lys Ile Asn Leu 
            420                 425                 430         


Thr Ala Ala Asn Lys Ile Pro Lys Ala Met Ile Asp Glu Phe Ile Leu 
        435                 440                 445             


Ser Pro Val Val Lys Arg Thr Phe Gly Gln Ala Ile Asn Leu Ile Asn 
    450                 455                 460                 


Lys Ile Ile Glu Lys Tyr Gly Val Pro Glu Asp Ile Ile Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Asn Asn Ser Lys Asp Lys Gln Lys Phe Ile Asn Glu Met 
                485                 490                 495     


Gln Lys Lys Asn Glu Asn Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly 
            500                 505                 510         


Lys Tyr Gly Asn Gln Asn Ala Lys Arg Leu Val Glu Lys Ile Arg Leu 
        515                 520                 525             


His Asp Glu Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro 
    530                 535                 540                 


Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile 
545                 550                 555                 560 


Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu 
                565                 570                 575     


Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln 
            580                 585                 590         


Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln 
        595                 600                 605             


His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys 
    610                 615                 620                 


Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln 
625                 630                 635                 640 


Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg 
                645                 650                 655     


Glu Leu Thr Asn Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn 
            660                 665                 670         


Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys 
        675                 680                 685             


Val Trp Lys Phe Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala 
    690                 695                 700                 


Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn 
705                 710                 715                 720 


Lys Lys Leu Lys Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu 
                725                 730                 735     


Thr Lys Gln Leu Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu 
            740                 745                 750         


Met Phe Ile Ile Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn 
        755                 760                 765             


Phe Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile 
785                 790                 795                 800 


Val Gln Thr Ile Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys 
                805                 810                 815     


Lys Gln Phe Asp Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp 
            820                 825                 830         


Pro Arg Thr Phe Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn 
        835                 840                 845             


Glu Lys Asn Pro Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu 
    850                 855                 860                 


Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys 
865                 870                 875                 880 


Tyr Ile Gly Asn Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe 
                885                 890                 895     


Lys Ser Ser Thr Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg 
            900                 905                 910         


Phe Asp Val Tyr Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser 
        915                 920                 925             


Tyr Leu Asp Val Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln 
    930                 935                 940                 


Lys Tyr Asp Lys Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys 
945                 950                 955                 960 


Phe Ile Ala Ser Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu 
                965                 970                 975     


Ile Tyr Lys Ile Ile Gly Val Asn Ser Asp Thr Arg Asn Met Ile Glu 
            980                 985                 990         


Leu Asp Leu Pro Asp Ile Arg Tyr  Lys Glu Tyr Cys Glu  Leu Asn Asn 
        995                 1000                 1005             


Ile Lys  Gly Glu Pro Arg Ile  Lys Lys Thr Ile Gly  Lys Lys Val 
    1010                 1015                 1020             


Asn Ser  Ile Glu Lys Leu Thr  Thr Asp Val Leu Gly  Asn Val Phe 
    1025                 1030                 1035             


Thr Asn  Thr Gln Tyr Thr Lys  Pro Gln Leu Leu Phe  Lys Arg Gly 
    1040                 1045                 1050             


Asn 
    


<210>  7
<211>  3171
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Original Gib11SpaCas9-1 polynucleotide

<400>  7
atgaaccaga aatttatcct gggtctggat attggtatta ccagcgttgg ttatggcctg       60

attgattacg aaaccaaaaa cattattgat gccggtgttc gtctgtttcc ggaagcaaat      120

gttgaaaata atgaaggtcg tcgtagcaaa cgtggtagcc gtcgtctgaa acgtcgtcgt      180

attcatcgtc tggaacgtgt taaactgctg ctgaccgaat atgatctgat taacaaagag      240

cagattccga ccagcaataa cccgtatcag attcgtgtta aaggtctgag cgaaatcctg      300

agcaaagatg aactggcaat tgcactgctg catctggcaa aacgccgtgg cattcataat      360

gttgatgttg cagcagataa agaagaaacc gcaagcgata gcctgagcac caaagatcag      420

attaacaaaa acgccaaatt tctggaaagc cgctatgttt gtgaactgca gaaagaacgt      480

ctggaaaatg aaggtcatgt tcgtggtgtt gaaaatcgct ttctgacgaa agatattgtg      540

cgtgaggcca aaaaaatcat cgatacccag atgcagtatt acccggaaat tgatgaaacc      600

ttcaaagaga aatatatcag cctggttgaa acccgtcgcg aatattttga aggtcctggt      660

cagggtagcc cgtttggttg gaatggtgat ctgaaaaaat ggtacgaaat gctgatgggt      720

cactgtacct attttccgca agaactgcgt agcgttaaat atgcctatag cgcagacctg      780

tttaatgcac tgaatgatct gaacaacctg attattcagc gcgataatag cgagaaactg      840

gaataccatg agaagtatca catcatcgag aacgtgttca agcagaaaaa aaagccgacg      900

ctgaaacaaa tcgcaaaaga gattggcgtt aacccggaag atattaaagg ttatcgtatt      960

accaaaagcg gcacaccgga gtttacatcc tttaaactgt tccacgatct gaaaaaagtg     1020

gtgaaagatc atgccatcct ggatgatatt gatctgctga atcagattgc agaaatcctg     1080

accatctatc aggataaaga tagcattgtt gcagaactgg gtcagctgga atatctgatg     1140

agcgaagccg ataaacagag cattagcgaa ctgaccggtt ataccggtac acatagcctg     1200

tcactgaaat gcatgaacat gattatcgat gaactgtggc atagcagcat gaaccagatg     1260

gaagttttta cctatctgaa tatgcgtccg aaaaagtatg agctgaaagg ttatcagcgt     1320

attccgaccg atatgattga tgatgcaatt ctgagtccgg ttgtgaaacg cacctttatt     1380

cagagcatca acgtgatcaa caaagtgatc gagaaatatg gcatccccga agatatcatt     1440

atcgaactgg cacgtgaaaa taactccgat gatcgcaaaa agttcatcaa caacctgcag     1500

aaaaagaatg aagcaacccg caaacgcatt aacgaaatta ttggtcagac cggtaatcag     1560

aatgccaaac gtattgtgga aaaaatccgt ctgcatgatc agcaagaggg caaatgtctg     1620

tatagcctgg aaagcattcc tctggaagat ctgctgaaca atccgaatca ttatgaagtg     1680

gatcacatta ttccgcgtag cgtgagcttt gataattcct atcataataa agtgctggtg     1740

aaacagagcg aaaactccaa aaaatccaac ctgacaccgt atcagtattt caatagcggc     1800

aaatccaaac tgagctacaa ccagtttaaa cagcatattc tgaacctgag caaaagccag     1860

gatcgcatca gcaagaagaa gaaggagtac ctgctggaag aacgcgatat taacaaattt     1920

gaagtgcaga aagaatttat caaccgcaac ctggttgata cccgttatgc aacccgtgaa     1980

ctgaccaatt atctgaaagc atatttcagc gccaacaaca tgaacgtgaa agtgaaaacg     2040

attaacggca gctttaccga ttatctgcgt aaagtgtgga aattcaaaaa agaacgcaac     2100

cacggctata aacatcatgc cgaagatgcc ctgattattg caaatgcaga tttcctgttt     2160

aaagaaaaca aaaaactgaa agccgtcaac agcgtgctgg aaaaaccgga aattgagaca     2220

aaacagctgg acattcaggt tgatagcgaa gataattaca gcgaaatgtt tatcatcccg     2280

aaacaggtgc aggatatcaa agattttcgc aacttcaaat atagccaccg cgttgacaaa     2340

aaacctaatc gtcagctgat taacgatacc ctgtatagca cccgcaaaaa agataacagc     2400

acctatattg tgcagaccat taaagacatc tacgccaaag ataataccac cctgaaaaaa     2460

cagttcgaca aaagcccaga aaaatttctg atgtatcagc atgatccgcg taccttcgaa     2520

aaactggaag ttattatgaa acagtatgcc aacgagaaaa atccgctggc caaatatcac     2580

gaagaaaccg gtgaatatct gaccaaatat tccaagaaga acaacggtcc gatcgttaaa     2640

tccctgaaat atatcggtaa taaactgggc agccatctgg atgttaccca tcagtttaaa     2700

agctccacaa agaagctggt taagctgtcc attaaaaact atcgcttcga tgtgtatctg     2760

accgagaaag gttataagtt tgtgaccatt gcctacctga atgtgttcaa aaaagacaac     2820

tattactata ttccgaaaga caaataccaa gaacttaaag agaagaagaa aatcaaggac     2880

accgatcagt ttatcgccag cttctataaa aacgatctga tcaagctgaa cggcgacctg     2940

tataaaatca ttggtgtgaa tagtgatgac cgcaacatca ttgagctgga ttattacgac     3000

atcaaataca aggattactg cgagatcaac aacattaaag gtgaaccgcg tatcaaaaag     3060

accattggca aaaaaacgga aagcatcgaa aagtttacca ccgatgttct gggtaatctg     3120

tatctgcata gtaccgaaaa agcaccgcag ctgattttca aacgcggtct g              3171


<210>  8
<211>  3171
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Original Gib11SpaCas9-3 polynucleotide

<400>  8
atgaaccaga aatttatcct gggtctggat attggtatta ccagcgttgg ttatggcctg       60

attgattacg aaaccaaaaa cattattgat gccggtgttc gtctgtttcc ggaagcaaat      120

gttgaaaata atgaaggtcg tcgtagcaaa cgtggtagcc gtcgtctgaa acgtcgtcgt      180

attcatcgtc tggaacgtgt taaactgctg ctgaccgaat atgatctgat taacaaagag      240

cagattccga ccagcaataa cccgtatcag attcgtgtta aaggtctgag cgaaatcctg      300

agcaaagatg aactggcaat tgcactgctg catctggcaa aacgccgtgg cattcataat      360

gttgatgttg cagcagataa agaagaaacc gcaagcgata gcctgagcac caaagatcag      420

attaacaaaa acgccaaatt tctggaaagc cgctatgttt gtgaactgca gaaagaacgt      480

ctggaaaatg aaggtcatgt tcgtggtgtt gaaaatcgct ttctgacgaa agatattgtg      540

cgtgaggcca aaaaaatcat cgatacccag atgcagtatt acccggaaat tgatgaaacc      600

ttcaaagaga aatatatcag cctggttgaa acccgtcgcg aatattttga aggtcctggt      660

cagggtagcc cgtttggttg gaatggtgat ctgaaaaaat ggtacgaaat gctgatgggt      720

cactgtacct attttccgca agaactgcgt agcgttaaat atgcctatag cgcagacctg      780

tttaatgcac tgaatgatct gaacaacctg attattcagc gcgataatag cgagaaactg      840

gaataccatg agaagtatca catcatcgag aacgtgttca agcagaaaaa aaagccgacg      900

ctgaaacaaa tcgcaaaaga gattggcgtt aacccggaag atattaaagg ttatcgtatt      960

accaaaagcg gcacaccgga gtttacatcc tttaaactgt tccacgatct gaaaaaagtg     1020

gtgaaagatc atgccatcct ggatgatatt gatctgctga atcagattgc agaaatcctg     1080

accatctatc aggataaaga tagcattgtt gcagaactgg gtcagctgga atatctgatg     1140

agcgaagccg ataaacagag cattagcgaa ctgaccggtt ataccggtac acatagcctg     1200

tcactgaaat gcatgaacat gattatcgat gaactgtggc atagcagcat gaaccagatg     1260

gaagttttta cctatctgaa tatgcgtccg aaaaagtatg agctgaaagg ttatcagcgt     1320

attccgaccg atatgattga tgatgcaatt ctgagtccgg ttgtgaaacg cacctttatt     1380

cagagcatca acgtgatcaa caaagtgatc gagaaatatg gcatccccga agatatcatt     1440

atcgaactgg cacgtgaaaa taactccgat gatcgcaaaa agttcatcaa caacctgcag     1500

aaaaagaatg aagcaacccg caaacgcatt aacgaaatta ttggtcagac cggtaatcag     1560

aatgccaaac gtattgtgga aaaaatccgt ctgcatgatc agcaagaggg caaatgtctg     1620

tatagcctgg aaagcattcc tctggaagat ctgctgaaca atccgaatca ttatgaagtg     1680

gatcacatta ttccgcgtag cgtgagcttt gataattcct atcataataa agtgctggtg     1740

aaacagagcg aaaactccaa aaaatccaac ctgacaccgt atcagtattt caatagcggc     1800

aaatccaaac tgagctacaa ccagtttaaa cagcatattc tgaacctgag caaaagccag     1860

gatcgcatca gcaagaagaa gaaggagtac ctgctggaag aacgcgatat caacaaattt     1920

gaagtccaga aagagtttat caaccgcaat ctggttgata cccgttatgc aacccgtgaa     1980

ctgaccagct atctgaaagc atatttcagc gccaataaca tggacgtgaa agtgaaaaca     2040

attaacggca gctttaccaa ccatctgcgt aaagtttggc gctttgataa atatcgcaac     2100

cacggctata aacatcatgc cgaagatgca ctgattattg ccaatgcaga tttcctgttc     2160

aaagaaaaca aaaaactgca gaacaccaac aagatcctgg aaaaaccgac cattgaaaac     2220

aacaccaaaa aagtgaccgt cgagaaagaa gaggattaca acaacgtttt tgaaaccccg     2280

aaactggtcg aggatattaa acagtatcgc gactataaat tcagccaccg cgttgataaa     2340

aaaccgaatc gtcagctgat taacgatacc ctgtatagca cccgtatgaa agatgagcat     2400

gattatattg tgcagaccat cacggatatc tatggcaaag ataataccaa cctgaaaaaa     2460

cagttcaaca aaaacccgga aaaatttctg atgtatcaga acgatccgaa aacctttgag     2520

aaactgagca tcatcatgaa acagtacagc gacgaaaaaa acccgctggc caaatattac     2580

gaagaaaccg gtgaatatct gaccaaatat agcaagaaaa acaacggtcc gatcgtgaaa     2640

aagatcaaac tgctgggtaa taaagtgggc aatcatctgg atgtgaccaa caaatatgaa     2700

aactccacga agaagctggt taagctgtcc attaaaaact atcgcttcga tgtgtatctg     2760

accgagaaag gttataagtt tgtgaccatt gcctacctga atgtgttcaa aaaagacaac     2820

tattactata ttccgaaaga caaataccaa gaacttaaag agaagaagaa aatcaaggac     2880

accgatcagt ttatcgccag cttctataaa aacgatctga tcaagctgaa cggcgacctg     2940

tataaaatca ttggtgtgaa tagtgatgac cgcaacatca ttgagctgga ttattacgac     3000

atcaaataca aggattactg cgagatcaac aacattaaag gtgaaccgcg tatcaaaaag     3060

accattggca aaaaaacgga aagcatcgaa aagtttacca ccgatgttct gggtaatctg     3120

tatctgcata gtaccgaaaa agcaccgcag ctgattttca aacgcggtct g              3171


<210>  9
<211>  3171
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Original E2Cas9 polynucleotide

<400>  9
atgaaccaga aatttatcct gggtctggat attggtatta ccagcgttgg ttatggcctg       60

attgattacg aaaccaaaaa cattattgat gccggtgttc gtctgtttcc ggaagcaaat      120

gttgaaaata atgaaggtcg tcgtagcaaa cgtggtagcc gtcgtctgaa acgtcgtcgt      180

attcatcgtc tggatcgtgt taaacatctg ctggcagaat atgatctgct ggatctgacc      240

aatattccga aaagcaccaa tccgtatcag acccgtgtta aaggtctgaa tgaaaagctg      300

agcaaagatg aactggttat tgcactgctg catattgcaa aacgccgtgg cattcataac      360

gttgatgttg cagcagataa agaagaaacc gcaagcgata gcctgagcac caaagatcag      420

attaacaaaa acgccaaatt tctggaaagc cgctatgttt gtgaactgca gaaagaacgt      480

ctggaaaatg aaggtcatgt tcgtggtgtt gaaaatcgct ttctgacgaa agatattgtg      540

cgtgaggcca aaaaaatcat cgatacccag atgcagtatt acccggaaat tgatgaaacc      600

ttcaaagaga aatatatcag cctggttgaa acccgtcgcg aatattttga aggtccgggt      660

aaaggtagcc cgtttggttg ggaaggtaat atcaagaaat ggtttgagca gatgatgggc      720

cactgtacct attttccaga agaactgcgt agcgtcaaat atagctattc agccgaactg      780

tttaacgccc tgaatgatct gaataatctg gtgattaccc gtgatgaaga tgccaaactg      840

aattatggtg agaaattcca gatcatcgaa aacgtgttca aacagaagaa aacaccgaac      900

ctgaaacaaa tcgccattga aattggtgtg catgaaaccg aaatcaaagg ttatcgtgtg      960

aacaaaagcg gtacaccgga atttaccgaa tttaaactgt atcatgacct gaaaagcatc     1020

gtgttcgata aaagcattct ggaaaatgaa gccatcctgg atcagattgc agaaattctg     1080

accatctatc aggatgagca gagcattaaa gaggaactga ataaactgcc ggaaatactg     1140

aacgaacagg ataaagcaga aatcgccaaa ctgattggtt ataatggcac ccatcgtctg     1200

agcctgaaat gtattcacct gattaatgaa gaactgtggc agaccagccg taatcagatg     1260

gaaattttca actacctgaa catcaaaccg aacaaagtgg atctgagtga gcagaacaaa     1320

atcccgaaag atatggtgaa cgactttatt ctgagtccgg ttgtgaaacg cacctttatt     1380

cagagcatca acgtgatcaa caaagtgatc gagaaatatg gcatccccga agatatcatt     1440

atcgaactgg cacgtgaaaa taactccgat gatcgcaaaa agttcatcaa caacctgcag     1500

aaaaagaatg aagcaacccg caaacgcatt aacgaaatta ttggtcagac cggtaatcag     1560

aatgccaaac gtattgtgga aaaaatccgt ctgcatgatc agcaagaggg taaatgtctg     1620

tatagcctga aagatatccc gctggaagat ctgctgcgca atccgaacaa ttatgatatc     1680

gaccatatta ttccgcgaag cgtgagcttt gatgatagca tgcataacaa agttctggtt     1740

cgtcgcgaac agaatgccaa aaagaataat cagaccccgt atcagtatct gaccagtggt     1800

tatgcagata tcaaatacag cgtgtttaag cagcatgttc tgaatctggc cgaaaataaa     1860

gatcgcatga ccaaaaaaaa gcgcgagtat ctgctggaag aacgcgacat taacaaattt     1920

gaagtgcaga aagaatttat caaccgcaac ctggttgata cccgttatgc aacccgtgaa     1980

ctgaccaatt atctgaaagc atatttcagc gccaacaaca tgaacgtgaa agtgaaaacg     2040

attaacggca gctttaccga ttatctgcgt aaagtgtgga aattcaaaaa agaacgcaac     2100

cacggctata aacatcatgc cgaagatgcc ctgattattg caaatgcaga tttcctgttt     2160

aaagaaaaca aaaaactgaa agccgtcaac agcgtgctgg aaaaaccgga aattgagaca     2220

aaacagctgg acattcaggt tgatagcgaa gataattaca gcgaaatgtt tatcatcccg     2280

aaacaggtgc aggatatcaa agattttcgc aacttcaaat atagccaccg cgttgacaaa     2340

aaacctaatc gtcagctgat taacgatacc ctgtatagca cccgcaaaaa agataacagc     2400

acctatattg tgcagaccat taaagacatc tacgccaaag ataataccac cctgaaaaaa     2460

cagttcgaca aaagcccaga aaaatttctg atgtatcagc atgatccgcg taccttcgaa     2520

aaactggaag ttattatgaa acagtatgcc aacgagaaaa atccgctggc caaatatcac     2580

gaagaaaccg gtgaatatct gaccaaatat tccaagaaga acaacggtcc gatcgttaaa     2640

tccctgaaat atatcggtaa taaactgggc agccatctgg atgttaccca tcagtttaaa     2700

agctccacaa agaagctggt taaactgtcc atcaaaccgt atcgctttga tgtgtatctg     2760

accgacaaag gctataaatt cattaccatc agctatctgg acgtgctgaa aaaagacaac     2820

tattattata tcccggaaca gaaatatgat aaactgaaac tgggtaaagc catcgataaa     2880

aacgccaaat ttatcgccag cttctacaaa aacgacctga ttaaactgga tggcgagatc     2940

tataaaatca tcggtgttaa tagcgacacc cgcaatatga ttgagctgga tctgccggat     3000

attcgctata aagaatattg cgaactgaac aacattaaag gcgaaccgcg tatcaaaaag     3060

accatcggca aaaaagtgaa tagcatcgag aaactgacca ccgatgttct gggtaatgtg     3120

tttaccaata cccagtatac caaacctcag ctgctgttca aacgcggtaa t              3171


<210>  10
<211>  3159
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Original F8Cas9 polynucleotide

<400>  10
atgaaccaga aatttatcct gggtctggat attggtatta ccagcgttgg ttatggcctg       60

attgattacg aaaccaaaaa cattattgat gccggtgttc gtctgtttcc ggaagcaaat      120

gttgaaaata atgaaggtcg tcgtagcaaa cgtggtagcc gtcgtctgaa acgtcgtcgt      180

attcatcgtc tggaacgtgt taaaagcctg ctgagcgaat ataagattat tagcggtctg      240

gcaccgacca ataatcagcc gtataacatt cgtgttaaag gtctgaccga acagctgacc      300

aaagatgaac tggcagttgc actgctgcat attgccaaac gccgtggcat tcataaaatc      360

gatgtgattg atagcaatga cgatgtgggt aatgaactga gcaccaaaga acagctgaac      420

aaaaatagca aactgctgaa agacaaattc gtgtgtcaga ttcagctgga acgtatgaat      480

gaaggccagg ttcgtggtga aaagaatcgc tttaaaaccg cagacatcat caaagaaatt      540

atccagctgc tgaacgtgca gaaaaacttc catcagctgg atgaaaactt catcaacaaa      600

tacatcgagc tggttgaaat gcgtcgcgaa tattttgaag gtcctggtca gggtagcccg      660

tttggttgga atggtgatct gaaaaaatgg tacgaaatgc tgatgggtca ctgtacctat      720

tttccgcaag aactgcgtag cgttaaatat gcctatagcg cagacctgtt taatgcactg      780

aatgatctga acaacctgat tattcagcgc gataatagcg agaaactgga ataccatgag      840

aagtatcaca tcatcgagaa cgtgttcaag cagaaaaaaa agccgacgct gaaacaaatc      900

gcaaaagaga ttggcgttaa cccggaagat attaaaggtt atcgtattac caaaagcggt      960

acaccggaat tcaccgaatt taaactgtat cacgatctga aaagcgtgct gtttgatcag     1020

agcattctgg aaaatgaaga tgtgctggac cagattgcag aaattctgac catttatcag     1080

gacaaagaca gcatcaaaag caaactgacc gaactggata ttctgctgaa tgaagaagat     1140

aaagagaaca ttgcacagct gaccggttat aacggcacac atcgcctgag cctgaaatgt     1200

attcgtctgg tactggaaga acagtggtat agcagccgta atcagatgga aatctttacc     1260

catctgaaca ttaaaccgaa gaaaatcaat ctgaccgcag ccaacaaaat tccgaaagcc     1320

atgattgatg agtttattct gagtccggtt gtgaaacgca cctttattca gagcatcaac     1380

gtgatcaaca aagtgatcga gaaatatggc atccccgaag atatcattat cgaactggca     1440

cgtgaaaata actccgatga tcgcaaaaag ttcatcaaca acctgcagaa aaagaatgaa     1500

gcaacccgca aacgcattaa cgaaattatt ggtcagaccg gtaatcagaa tgccaaacgt     1560

attgtggaaa aaatccgtct gcatgatcag caagagggga aatgtctgta tagcctggaa     1620

agcattgccc tgatggatct gctgaataac ccgcagaatt atgaagtgga tcatattatt     1680

ccgcgtagcg tggcatttga taattccatt cataacaaag tgctggtgaa gcagatcgag     1740

aatagcaaaa aaggtaatcg tacgccgtat cagtatctga atagcagtga tgcaaaactg     1800

agctacaacc agtttaaaca gcatattctg aatctgagca aaagcaaaga tcgcatcagc     1860

aaaaaaaaga aggactacct gctggaagaa cgcgatatca acaaatttga agtccagaaa     1920

gagtttatca accgcaatct ggttgatacc cgttatgcaa cccgtgaact gaccagctat     1980

ctgaaagcat atttcagcgc caataacatg gacgtgaaag tgaaaacaat taacggcagc     2040

tttaccaacc atctgcgtaa agtttggcgc tttgataaat atcgcaacca cggctataaa     2100

catcatgcag aagatgccct gattattgca aatgcagatt tcctgtttaa agaaaacaaa     2160

aaactgaaag ccgtcaacag cgtgctggaa aaaccggaaa ttgagacaaa acagctggac     2220

attcaggttg atagcgaaga taattacagc gaaatgttta tcatcccgaa acaggtgcag     2280

gatatcaaag attttcgcaa cttcaaatat agccaccgcg ttgacaaaaa acctaatcgt     2340

cagctgatta acgataccct gtatagcacc cgcaaaaaag ataacagcac ctatattgtg     2400

cagaccatta aagacatcta cgccaaagat aataccaccc tgaaaaaaca gttcgacaaa     2460

agcccagaaa aatttctgat gtatcagcat gatccgcgta ccttcgaaaa actggaagtt     2520

attatgaaac agtatgccaa cgagaaaaat ccgctggcca aatatcacga agaaaccggt     2580

gaatatctga ccaaatattc caagaagaac aacggtccga tcgttaaatc cctgaaatat     2640

atcggtaata aactgggcag ccatctggat gttacccatc agtttaaaag ctccacaaag     2700

aagctggtta aactgtccat caaaccgtat cgctttgatg tgtatctgac cgacaaaggc     2760

tataaattca ttaccatcag ctatctggac gtgctgaaaa aagacaacta ttattatatc     2820

ccggaacaga aatatgataa actgaaactg ggtaaagcca tcgataaaaa cgccaaattt     2880

atcgccagct tctacaaaaa cgacctgatt aaactggatg gcgagatcta taaaatcatc     2940

ggtgttaata gcgacacccg caatatgatt gagctggatc tgccggatat tcgctataaa     3000

gaatattgcg aactgaacaa cattaaaggc gaaccgcgta tcaaaaagac catcggcaaa     3060

aaagtgaata gcatcgagaa actgaccacc gatgttctgg gtaatgtgtt taccaatacc     3120

cagtatacca aacctcagct gctgttcaaa cgcggtaat                            3159


<210>  11
<211>  3162
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Original P2H12Cas9 polynucleotide

<400>  11
atgaaccaga aatttatcct gggtctggat attggtatta ccagcgttgg ttatggcctg       60

attgattacg aaaccaaaaa cattattgat gccggtgttc gtctgtttcc ggaagcaaat      120

gttgaaaata atgaaggtcg tcgtagcaaa cgtggtagcc gtcgtctgaa acgtcgtcgt      180

attcatcgtc tggaacgtgt taaaaaactg ctggaagatt ataacctgct ggatcagagc      240

cagattccgc agagcaccaa tccgtatgca attcgtgtta aaggtctgag cgaagcactg      300

agcaaagatg aactggttat tgcactgctg catattgcaa aacgccgtgg cattcataat      360

atcaatgtta gcagcgaaga tgaggatgca agcaatgaac tgagcaccaa agaacaaatt      420

aaccgcaata ataagctgct gaaggacaaa tatgtttgcg aagttcagct gcagcgtctg      480

aaagaaggtc agattcgcgg agaaaaaaat cgctttaaaa ccaccgatat cctgaaagaa      540

attgatcagc tgcttaaagt gcagaaggat tatcataacc tggacatcga tttcatcaac      600

cagtacaaag aaatcgttga aacccgtcgc gaatattttg aaggtccggg taaaggtagc      660

ccgtatggtt gggaaggtga tccgaaagca tggtatgaaa ccctgatggg tcattgtacc      720

tattttccgg atgaactgcg tagcgttaaa tatgcctata gcgcagacct gtttaatgca      780

ctgaatgatc tgaataacct ggtgattcag cgtgatggtc tgagcaaact ggaatatcat      840

gagaaatatc acatcatcga aaacgtgttc aaacagaaga agaaaccgac cctgaaacaa      900

atcgccaacg aaattaatgt gaacccggaa gatattaaag gctaccgtat taccaaaagc      960

ggcaaaccgg aatttacatc ctttaaactg ttccacgatc tgaaaaaagt ggtgaaagat     1020

catgccatcc tggatgatat tgatctgctg aatcagattg cagaaatcct gaccatctat     1080

caggataaag atagcattgt tgcagaactg ggtcagctgg aatatctgat gagcgaagcc     1140

gataaacaga gcattagcga actgaccggt tataccggta cacatagcct gtcactgaaa     1200

tgcatgaaca tgattatcga tgaactgtgg catagcagca tgaaccagat ggaagttttt     1260

acctatctga atatgcgtcc gaaaaagtat gagctgaaag gttatcagcg tattccgacc     1320

gatatgattg atgatgcaat tctgagtccg gttgtgaaac gcacctttat tcagagcatc     1380

aacgtgatca acaaagtgat cgagaaatat ggcatccccg aagatatcat tatcgaactg     1440

gcacgtgaaa ataactccga tgatcgcaaa aagttcatca acaacctgca gaaaaagaat     1500

gaagcaaccc gcaaacgcat taacgaaatt attggtcaga ccggtaatca gaatgccaaa     1560

cgtattgtgg aaaaaatccg tctgcatgat cagcaagagg gtaaatgtct gtatagcctg     1620

gaaagcattc ctctggaaga tctgctgaac aatccgaatc attatgaagt ggatcacatt     1680

attccgcgta gcgtgagctt tgataattcc tatcataata aagtgctggt gaaacagagc     1740

gaaaactcca aaaaatccaa cctgacaccg tatcagtatt tcaatagcgg caaatccaaa     1800

ctgagctaca accagtttaa acagcatatt ctgaacctga gcaaaagcca ggatcgcatc     1860

agcaagaaga agaaggagta cctgctggaa gaacgcgata ttaacaaatt tgaagtgcag     1920

aaagaattta tcaaccgcaa cctggttgat acccgttatg caacccgtga actgaccaat     1980

tatctgaaag catatttcag cgccaacaac atgaacgtga aagtgaaaac gattaacggc     2040

agctttaccg attatctgcg taaagtgtgg aaattcaaaa aagaacgcaa ccacggctat     2100

aaacatcatg ccgaagatgc cctgattatt gcaaatgcag atttcctgtt taaagaaaac     2160

aaaaaactga aagccgtcaa cagcgtgctg gaaaaaccgg aaattgagac aaaacagctg     2220

gacattcagg ttgatagcga agataattac agcgaaatgt ttatcatccc gaaacaggtg     2280

caggatatca aagattttcg caacttcaaa tatagccacc gcgttgacaa aaaacctaat     2340

cgtcagctga ttaacgatac cctgtatagc acccgcaaaa aagataacag cacctatatt     2400

gtgcagacca ttaaagacat ctacgccaaa gataatacca ccctgaaaaa acagttcgac     2460

aaaagcccag aaaaatttct gatgtatcag catgatccgc gtaccttcga aaaactggaa     2520

gttattatga aacagtatgc caacgagaaa aatccgctgg ccaaatatca cgaagaaacc     2580

ggtgaatatc tgaccaaata ttccaagaag aacaacggtc cgatcgttaa atccctgaaa     2640

tatatcggta ataaactggg cagccatctg gatgttaccc atcagtttaa aagctccaca     2700

aagaagctgg ttaaactgtc catcaaaccg tatcgctttg atgtgtatct gaccgacaaa     2760

ggctataaat tcattaccat cagctatctg gacgtgctga aaaaagacaa ctattattat     2820

atcccggaac agaaatatga taaactgaaa ctgggtaaag ccatcgataa aaacgccaaa     2880

tttatcgcca gcttctacaa aaacgacctg attaaactgg atggcgagat ctataaaatc     2940

atcggtgtta atagcgacac ccgcaatatg attgagctgg atctgccgga tattcgctat     3000

aaagaatatt gcgaactgaa caacattaaa ggcgaaccgc gtatcaaaaa gaccatcggc     3060

aaaaaagtga atagcatcga gaaactgacc accgatgttc tgggtaatgt gtttaccaat     3120

acccagtata ccaaacctca gctgctgttc aaacgcggta at                        3162


<210>  12
<211>  3162
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Original SluCas9 polynucleotide

<400>  12
atgaaccaga aatttatcct gggtctggat attggtatta ccagcgttgg ttatggcctg       60

attgattacg aaaccaaaaa cattattgat gccggtgttc gtctgtttcc ggaagcaaat      120

gttgaaaata atgaaggtcg tcgtagcaaa cgtggtagcc gtcgtctgaa acgtcgtcgt      180

attcatcgtc tggaacgtgt taaaaaactg ctggaagatt ataacctgct ggatcagagc      240

cagattccgc agagcaccaa tccgtatgca attcgtgtta aaggtctgag cgaagcactg      300

agcaaagatg aactggttat tgcactgctg catattgcaa aacgccgtgg cattcataaa      360

atcgatgtga ttgatagcaa tgacgatgtg ggtaatgaac tgagcaccaa agaacagctg      420

aacaaaaata gcaaactgct gaaagacaaa ttcgtgtgtc agattcagct ggaacgtatg      480

aatgaaggcc aggttcgtgg tgaaaagaat cgctttaaaa ccgcagacat catcaaagaa      540

attatccagc tgctgaacgt gcagaaaaac ttccatcagc tggatgaaaa cttcatcaac      600

aaatacatcg agctggttga aatgcgtcgc gaatattttg aaggtccggg taaaggtagc      660

ccgtatggtt gggaaggtga tccgaaagca tggtatgaaa ccctgatggg tcattgtacc      720

tattttccgg atgaactgcg tagcgttaaa tatgcctata gcgcagacct gtttaatgca      780

ctgaatgatc tgaataacct ggtgattcag cgtgatggtc tgagcaaact ggaatatcat      840

gagaaatatc acatcatcga aaacgtgttc aaacagaaga agaaaccgac cctgaaacaa      900

atcgccaacg aaattaatgt gaacccggaa gatattaaag gctaccgtat taccaaaagc      960

ggtaaaccgc agttcaccga atttaaactg tatcacgatc tgaaaagcgt gctgtttgat     1020

cagagcattc tggaaaatga agatgtgctg gaccagattg cagaaattct gaccatttat     1080

caggacaaag acagcatcaa aagcaaactg accgaactgg atattctgct gaatgaagaa     1140

gataaagaga acattgcaca gctgaccggt tataccggca cccatcgtct gagcctgaaa     1200

tgtattcgtc tggtactgga agaacagtgg tatagcagcc gtaatcagat ggaaatcttt     1260

acccatctga acattaaacc gaagaaaatc aatctgaccg cagccaacaa aattccgaaa     1320

gccatgattg atgagtttat tctgagtccg gttgtgaaac gtacctttgg tcaggcaatt     1380

aacctgatca acaaaatcat tgaaaaatat ggcgtgcctg aggatatcat tattgaactg     1440

gcacgtgaaa acaacagcaa agataaacag aaattcatca acgagatgca gaagaagaac     1500

gaaaataccc gcaaacggat taacgagatc attggcaaat atggtaatca gaatgccaaa     1560

cgcctggtgg aaaaaattcg tctgcatgat gaacaagagg gcaaatgtct gtatagcctg     1620

gaaagcattc ctctggaaga tctgctgaac aatccgaatc attatgaagt ggatcacatt     1680

attccgcgta gcgtgagctt tgataattcc tatcataata aagtgctggt gaaacagagc     1740

gaaaactcca aaaaatccaa cctgacaccg tatcagtatt tcaatagcgg caaatccaaa     1800

ctgagctaca accagtttaa acagcatatt ctgaacctga gcaaaagcca ggatcgcatc     1860

agcaagaaga agaaggagta cctgctggaa gaacgcgaca tcaacaaatt tgaagtgcag     1920

aaagaattta tcaaccgcaa cctggttgat acccgttatg caacccgtga actgaccaat     1980

tatctgaaag catatttcag cgccaacaac atgaacgtga aagtgaaaac gattaacggc     2040

agctttaccg attatctgcg taaagtgtgg aaattcaaaa aagaacgcaa ccacggctat     2100

aaacatcatg ccgaagatgc cctgattatt gcaaatgcag atttcctgtt taaagaaaac     2160

aaaaaactga aagccgtcaa cagcgtgctg gaaaaaccgg aaattgagac aaaacagctg     2220

gacattcagg ttgatagcga agataattac agcgaaatgt ttatcatccc gaaacaggtg     2280

caggatatca aagattttcg caacttcaaa tatagccacc gcgttgacaa aaaacctaat     2340

cgtcagctga ttaacgatac cctgtatagc acccgcaaaa aagataacag cacctatatt     2400

gtgcagacca ttaaagacat ctacgccaaa gataatacca ccctgaaaaa acagttcgac     2460

aaaagcccag aaaaatttct gatgtatcag catgatccgc gtaccttcga aaaactggaa     2520

gttattatga aacagtatgc caacgagaaa aatccgctgg ccaaatatca cgaagaaacc     2580

ggtgaatatc tgaccaaata ttccaagaag aacaacggtc cgatcgttaa atccctgaaa     2640

tatatcggta ataaactggg cagccatctg gatgttaccc atcagtttaa aagctccaca     2700

aagaagctgg ttaaactgtc catcaaaccg tatcgctttg atgtgtatct gaccgacaaa     2760

ggctataaat tcattaccat cagctatctg gacgtgctga aaaaagacaa ctattattat     2820

atcccggaac agaaatatga taaactgaaa ctgggtaaag ccatcgataa aaacgccaaa     2880

tttatcgcca gcttctacaa aaacgacctg attaaactgg atggcgagat ctataaaatc     2940

atcggtgtta atagcgacac ccgcaatatg attgagctgg atctgccgga tattcgctat     3000

aaagaatatt gcgaactgaa caacattaaa ggcgaaccgc gtatcaaaaa gaccatcggc     3060

aaaaaagtga atagcatcga gaaactgacc accgatgttc tgggtaatgt gtttaccaat     3120

acccagtata ccaaacctca gctgctgttc aaacgcggta at                        3162


<210>  13
<211>  3171
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized Gib11SpaCas9-1 polynucleotide

<400>  13
atgaaccaga agttcattct cggtctggac attggcatta ctagcgtggg atacggcttg       60

attgactacg agactaagaa catcatcgat gccggcgtcc gcctgttccc ggaagccaac      120

gtggagaaca atgagggccg gaggtcgaag agaggctccc gccgcctgaa gcggcggcga      180

atccaccggc tggagagagt gaagctgctg ctcaccgaat acgacctgat caacaaagaa      240

cagatcccga cctccaacaa cccgtaccag atcagagtga agggtctgtc agaaatcctg      300

tccaaggacg aactggcaat cgccctgctg cacctggcca agcggcgcgg aatccacaac      360

gtggatgtgg ctgccgacaa ggaagaaacc gcttccgact ccctgagcac taaggaccag      420

atcaacaaga acgccaagtt cttggagtcc cgctacgtgt gcgagctgca gaaggaacgg      480

ctggaaaacg aaggtcacgt gcgcggagtg gaaaaccggt tcctgacaaa ggacattgtg      540

cgcgaagcga agaagatcat tgatacccaa atgcagtact accctgaaat cgacgagact      600

ttcaaggaaa agtacatttc cctggtggaa acccggcggg aatacttcga aggccccgga      660

cagggatcgc cgttcggatg gaacggggac ctcaagaagt ggtacgagat gctgatgggg      720

cactgtacct actttccgca agaactgcgc tccgtgaagt acgcgtactc cgcggatctc      780

ttcaacgcgt tgaatgacct gaacaacctg atcattcaga gagacaattc cgaaaagctc      840

gagtaccacg agaagtatca catcatcgag aatgtgttca agcagaagaa gaaaccgacc      900

ctcaagcaaa tcgccaagga gattggcgtc aacccagagg acatcaaggg atatcggatt      960

accaagagcg gcactcccga gtttacctct ttcaagctgt ttcatgatct gaagaaagtc     1020

gtgaaggacc atgccattct cgacgacatt gatctcctga atcagatcgc agagatcctg     1080

actatctacc aagacaagga ctcgattgtg gcagagctgg gtcagctcga atacctgatg     1140

tccgaggccg acaagcagtc catctccgaa ctgacagggt acacggggac tcatagcctg     1200

tcgctgaagt gcatgaacat gatcattgat gaactgtggc acagctccat gaaccaaatg     1260

gaagtgttta cctacctcaa catgcgccct aagaagtacg aactgaaagg ctaccagcgc     1320

atccccaccg acatgatcga cgacgcgatc ttgtcccctg tggtcaagag gaccttcatt     1380

caatccatca acgtgatcaa caaggtcatc gaaaagtacg gaataccaga agatatcatc     1440

attgagctcg ctcgggagaa caactcggat gaccggaaga agttcatcaa caatcttcag     1500

aagaagaacg aagcgactcg gaaacggatc aacgagatca tcggacagac cggaaaccag     1560

aacgccaaac ggattgtcga aaagattaga ctgcacgacc agcaggaagg gaagtgcctg     1620

tactcactcg agtcaatacc gctcgaggac ctgttgaaca accctaacca ctatgaagtg     1680

gaccacatca tccctcggtc cgtgagcttc gacaactcgt accacaacaa agtgctcgtg     1740

aagcagtccg aaaactccaa gaaatccaac ctgaccccgt accaatactt caattcggga     1800

aagtcgaagc tgtcgtacaa ccagttcaaa caacacatac tcaaccttag caaaagccag     1860

gatcgcattt ccaagaagaa gaaggaatac ctcctcgagg aaagggacat caacaagttc     1920

gaagtgcaga aagagttcat caatcgcaac ttggtggata ccagatatgc cacccgggaa     1980

ctcaccaact atctcaaggc ctacttttcc gccaacaaca tgaacgtgaa ggtcaagacc     2040

atcaacgggt ccttcactga ctacctgaga aaggtctgga agttcaagaa ggaacgcaac     2100

cacggataca agcaccacgc tgaggacgct ctgatcatcg ccaatgccga cttcctgttc     2160

aaggaaaaca agaagctgaa agctgtcaac tcagtgctgg aaaagcctga aatcgagact     2220

aagcagctgg atatccaagt ggactctgag gacaactaca gcgagatgtt catcatccct     2280

aaacaagtgc aggatatcaa ggactttcgc aacttcaagt actcacaccg ggtggacaag     2340

aaaccgaata gacagctgat caacgacacg ttgtattcca cccggaagaa ggataactca     2400

acctacattg tgcagactat caaggatatc tacgccaaag ataacactac tctgaagaaa     2460

caattcgaca agtccccaga gaagttcctg atgtaccagc acgacccccg aacctttgag     2520

aagcttgaag tgatcatgaa gcagtacgcc aacgagaaga acccgctggc caagtaccat     2580

gaagaaaccg gagaatacct gaccaagtac agcaagaaga acaacggtcc cattgtcaag     2640

agcctgaagt acatcggcaa caagctggga tcccacctcg acgtgacaca tcagttcaag     2700

tcgtcgacta agaagcttgt gaagctgtca atcaagaact atagattcga cgtgtacttg     2760

accgaaaagg gatacaagtt cgtgaccata gcctatctga acgtgttcaa gaaagataac     2820

tactactaca tccccaagga caagtaccag gagctcaaag aaaagaagaa gatcaaagac     2880

accgaccagt tcattgcctc cttctacaag aacgacctga tcaaactgaa cggcgacctc     2940

tacaagatca ttggagtgaa cagcgatgac aggaacatca ttgagctgga ctactacgac     3000

atcaagtaca aggactactg cgagatcaac aacatcaagg gcgaaccccg gatcaagaaa     3060

actattggaa agaaaaccga gtccattgag aagttcacca ctgacgtgct gggaaacctt     3120

tacctccact ccaccgagaa ggcaccacaa ctgatcttca agcgcggcct g              3171


<210>  14
<211>  3171
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized Gib11SpaCas9-3 polynucleotide

<400>  14
atgaaccaga agttcattct cggtctggac attggcatta ctagcgtggg atacggcttg       60

attgactacg agactaagaa catcatcgat gccggcgtcc gcctgttccc ggaagccaac      120

gtggagaaca atgagggccg gaggtcgaag agaggctccc gccgcctgaa gcggcggcga      180

atccaccggc tggagagagt gaagctgctg ctcaccgaat acgacctgat caacaaagaa      240

cagatcccga cctccaacaa cccgtaccag atcagagtga agggtctgtc agaaatcctg      300

tccaaggacg aactggcaat cgccctgctg cacctggcca agcggcgcgg aatccacaac      360

gtggatgtgg ctgccgacaa ggaagaaacc gcttccgact ccctgagcac taaggaccag      420

atcaacaaga acgccaagtt cttggagtcc cgctacgtgt gcgagctgca gaaggaacgg      480

ctggaaaacg aaggtcacgt gcgcggagtg gaaaaccggt tcctgacaaa ggacattgtg      540

cgcgaagcga agaagatcat tgatacccaa atgcagtact accctgaaat cgacgagact      600

ttcaaggaaa agtacatttc cctggtggaa acccggcggg aatacttcga aggccccgga      660

cagggatcgc cgttcggatg gaacggggac ctcaagaagt ggtacgagat gctgatgggg      720

cactgtacct actttccgca agaactgcgc tccgtgaagt acgcgtactc cgcggatctc      780

ttcaacgcgt tgaatgacct gaacaacctg atcattcaga gagacaattc cgaaaagctc      840

gagtaccacg agaagtatca catcatcgag aatgtgttca agcagaagaa gaaaccgacc      900

ctcaagcaaa tcgccaagga gattggcgtc aacccagagg acatcaaggg atatcggatt      960

accaagagcg gcactcccga gtttacctct ttcaagctgt ttcatgatct gaagaaagtc     1020

gtgaaggacc atgccattct cgacgacatt gatctcctga atcagatcgc agagatcctg     1080

actatctacc aagacaagga ctcgattgtg gcagagctgg gtcagctcga atacctgatg     1140

tccgaggccg acaagcagtc catctccgaa ctgacagggt acacggggac tcatagcctg     1200

tcgctgaagt gcatgaacat gatcattgat gaactgtggc acagctccat gaaccaaatg     1260

gaagtgttta cctacctcaa catgcgccct aagaagtacg aactgaaagg ctaccagcgc     1320

atccccaccg acatgatcga cgacgcgatc ttgtcccctg tggtcaagag gaccttcatt     1380

caatccatca acgtgatcaa caaggtcatc gaaaagtacg gtattccaga agatatcatc     1440

attgagctcg ctcgggagaa caactcggat gaccggaaga agttcatcaa caatcttcag     1500

aagaagaacg aagcgactcg gaaacggatc aacgagatca tcggacagac cggaaaccag     1560

aacgccaaac ggattgtcga aaagattaga ctgcacgacc agcaggaagg gaagtgcctg     1620

tactcactcg agtcaatacc gctcgaggac ctgttgaaca accctaacca ctatgaagtg     1680

gaccacatca tccctcggtc cgtgagcttc gacaactcgt accacaacaa agtgctcgtg     1740

aagcagtccg aaaactccaa gaaatccaac ctgaccccgt accaatactt caattcggga     1800

aagtcgaagc tgtcgtacaa ccagttcaaa caacacatac tcaaccttag caaaagccag     1860

gatcgcattt ccaagaagaa gaaggaatac ctcctcgagg aaagggacat caacaagttc     1920

gaagtgcaga aagagttcat caatcgcaac ttggtggata ccagatatgc cacccgggaa     1980

ctcaccagct atctcaaggc ctacttttcc gccaacaaca tggacgtgaa ggtcaagacc     2040

atcaacgggt ccttcactaa ccatctgaga aaggtctggc ggtttgacaa gtaccgcaac     2100

cacggataca agcaccacgc tgaagacgct ctgatcatcg ccaatgccga cttcctgttc     2160

aaggaaaaca agaagctgca gaacacgaac aagattctgg aaaagcctac cattgagaac     2220

aacactaaga aggtcaccgt ggagaaggaa gaggactaca acaacgtgtt cgaaactcct     2280

aaactggtgg aggatatcaa gcaataccgc gactacaagt tctcacaccg ggtggacaag     2340

aaaccgaata gacagctgat caacgacacg ttgtattcca cccggatgaa ggatgagcat     2400

gactacattg tgcagactat caccgatatc tacggaaaag ataacactaa cctgaagaaa     2460

caattcaaca agaacccaga gaagttcctg atgtaccaga acgaccccaa gacctttgag     2520

aagctttcca tcatcatgaa gcagtactcc gacgagaaga acccgctggc caagtactac     2580

gaagaaaccg gagaatacct gaccaagtac agcaagaaga acaacggtcc cattgtcaag     2640

aagatcaagc tgctcggcaa caaggtcgga aaccacctcg acgtgacaaa caagtacgag     2700

aactcgacta agaagcttgt gaagctgtca atcaagaact atagattcga cgtgtacttg     2760

accgaaaagg gatacaagtt cgtgaccata gcctatctga acgtgttcaa gaaagataac     2820

tactactaca tccccaagga caagtaccag gagctcaaag aaaagaagaa gatcaaagac     2880

accgaccagt tcattgcctc cttctacaag aacgacctga tcaaactgaa cggcgacctc     2940

tacaagatca ttggagtgaa cagcgatgac aggaacatca ttgagctgga ctactacgac     3000

atcaagtaca aggactactg cgagatcaac aacatcaagg gcgaaccccg gatcaagaaa     3060

actattggaa agaaaaccga gtccattgag aagttcacca ctgacgtgct gggaaacctt     3120

tacctccact ccaccgagaa ggcaccacaa ctgatcttca agcgcggcct g              3171


<210>  15
<211>  3171
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized E2Cas9 polynucleotide

<400>  15
atgaaccaaa agttcattct ggggctcgat atcggcatca cctccgtggg atatggtctg       60

atcgactacg agactaagaa catcatcgac gctggagtgc gactgttccc ggaagcgaac      120

gtggagaaca acgaaggccg cagatccaag cgcgggtcca gaaggctcaa gaggcggagg      180

atccatagac tcgacagagt gaagcacctc cttgccgaat acgatctgtt ggaccttacc      240

aacattccca agagcaccaa cccgtaccaa accagagtga agggcctgaa cgaaaagctg      300

tcgaaagatg aactggtcat tgccctgctg catattgcca aacggcgcgg aatccataac      360

gtggacgtgg ccgctgacaa ggaagagact gcgtccgact cgctgtcaac caaggaccag      420

atcaacaaga acgccaaatt cctggaaagc cgctacgtct gcgagcttca aaaagaacgg      480

ctggagaacg agggacacgt caggggagtg gagaaccggt tcctgaccaa ggacatcgtg      540

cgggaagcca agaagatcat cgacacccaa atgcagtatt atccggaaat tgatgaaact      600

tttaaggaga agtacatttc cctggtggaa actcggaggg agtacttcga gggacctgga      660

aagggatccc ctttcggctg ggaagggaac attaagaagt ggtttgaaca gatgatgggc      720

cattgcactt actttccgga agaactccgg tccgtgaagt actcatactc tgccgagctg      780

ttcaatgcac tcaacgacct taacaacttg gtgatcaccc gcgatgaaga tgccaagttg      840

aactacggag aaaagttcca gatcatcgag aacgtgttca agcagaaaaa gaccccaaat      900

ctgaagcaga ttgccatcga aattggcgtg cacgagactg agatcaaggg ataccgggtc      960

aacaagtccg gcacgccaga gttcaccgag ttcaagctgt accacgatct gaagtcgatc     1020

gtgtttgaca agtccatcct ggaaaacgaa gccattctgg accagattgc tgagatcctg     1080

accatctacc aggacgagca atcgattaag gaagaactga acaagctccc cgagattctg     1140

aacgaacagg ataaggccga gatcgcgaag ctcattggtt acaatggtac ccaccgcttg     1200

tcccttaagt gcatccatct gatcaatgag gaactgtggc agaccagccg gaaccagatg     1260

gagatcttca attacttgaa catcaagccg aacaaggtgg acctgtccga acagaacaag     1320

atacccaagg acatggtcaa cgactttatc ctctcaccgg tggtcaagcg caccttcatt     1380

caatctatca acgtgatcaa caaggtcatc gagaagtacg gcattcctga ggatatcatc     1440

atcgagctgg ctcgggagaa caactcagac gataggaaga agttcattaa caacctccag     1500

aaaaagaacg aggccactcg caagcggatt aatgagatca tcggtcagac cgggaaccag     1560

aacgccaagc ggatcgtgga aaagattcgg ctccacgacc aacaggaggg aaagtgtctg     1620

tactcgctga aggacattcc cctggaggac ctcctgagga acccaaacaa ctacgacatc     1680

gatcacataa tcccccgcag cgtgtcattc gacgattcca tgcataacaa ggtcctcgtg     1740

cggagagagc agaatgccaa gaagaacaac cagactccgt accagtacct gacgtccggc     1800

tacgcagaca tcaagtactc agtgttcaaa cagcacgtgc tcaacctggc cgagaacaag     1860

gacaggatga ccaagaagaa gcgcgaatac cttctcgagg aacgggatat caataagttc     1920

gaggtgcaga aggagtttat caatagaaac ctggtggaca ctcgctatgc cacccgcgaa     1980

ctgaccaact acctgaaggc gtacttctcc gccaacaaca tgaacgtgaa ggtcaaaact     2040

attaacggca gcttcaccga ctatctgcgc aaggtctgga agttcaagaa ggaacgcaac     2100

cacggttaca agcaccacgc ggaagatgcg ctgattatcg ccaacgctga cttcctgttc     2160

aaggaaaaca agaagctcaa ggccgtgaac tcagtgctcg agaagcctga aatcgagact     2220

aagcagctgg acatccaggt cgattcggaa gataactact ccgaaatgtt catcatccct     2280

aagcaagtgc aggacatcaa ggacttcagg aatttcaagt acagccatcg cgtggacaag     2340

aagccaaaca gacagctgat caacgataca ctgtattcca cccggaagaa ggacaactcc     2400

acctacatcg tccaaaccat taaggacatc tacgcaaagg acaacaccac gcttaagaag     2460

cagttcgaca agagccccga aaagttcctc atgtaccagc acgaccccag aaccttcgag     2520

aagcttgaag tgatcatgaa gcagtacgcc aacgaaaaga acccactggc taagtaccac     2580

gaggaaaccg gcgaatacct gaccaagtac tccaaaaaga acaacggacc gatcgtcaag     2640

tccctgaagt acattgggaa caagctcggc tcgcacctcg atgtgaccca ccagttcaag     2700

tcctcgacca aaaagctcgt gaagctgtcc atcaagccgt accggttcga cgtgtacctg     2760

actgacaagg gatataagtt catcaccatt tcctacctcg acgtgttgaa gaaggataac     2820

tactactaca ttccggaaca gaagtacgac aagctcaagc tcggaaaggc catcgacaaa     2880

aatgcgaagt tcatcgcgag cttctacaag aatgacttga tcaagctgga tggcgaaatc     2940

tacaagatca tcggggtcaa ctccgatacc cgcaacatga ttgagctgga tctgcccgac     3000

attcggtaca aggaatactg cgagctgaac aacatcaagg gagaaccgcg gatcaagaaa     3060

accatcggaa agaaagtgaa cagcatcgag aaactgacta ctgacgtcct gggaaacgtg     3120

ttcaccaaca cacaatacac caaaccccag ctgctgttta agcgcgggaa c              3171


<210>  16
<211>  3159
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized F8Cas9 polynucleotide

<400>  16
atgaaccaaa agttcattct ggggctcgat atcggcatca cctccgtggg atatggtctg       60

atcgactacg agactaagaa catcatcgac gctggagtgc gactgttccc ggaagcgaac      120

gtggagaaca acgaaggccg cagatccaag cgcgggtcca gaaggctcaa gaggcggagg      180

atccatagac tcgagagagt gaagtcgctc ctttcggaat acaagattat cagcggtctt      240

gcccccacca acaaccaacc gtacaacatc agagtgaagg gcctgaccga acagctgacc      300

aaagatgaac tggccgtcgc cctgctgcat attgccaaac ggcgcggaat ccataagatc      360

gacgtgattg acagcaacga tgacgtggga aacgagctgt caaccaagga acagcttaac      420

aagaacagca aattgctgaa ggacaagttt gtctgccaaa ttcaactgga acggatgaac      480

gagggacaag tcaggggaga gaaaaaccgg ttcaagaccg ccgacatcat caaggagatc      540

atccaactgc tgaacgtgca gaagaacttc caccaactgg atgaaaactt cattaacaag      600

tacattgaac tggtggaaat gcggagggag tacttcgagg gacctggaca gggatcccct      660

ttcggctgga atggggacct taagaagtgg tacgaaatgt tgatgggcca ttgcacttac      720

tttccgcaag aactccggtc cgtgaagtac gcatactctg ccgacctgtt caatgcactc      780

aacgacctta acaacttgat catccagcgc gataactcgg aaaagttgga ataccacgaa      840

aagtatcaca tcatcgagaa cgtgttcaag cagaaaaaga agccaactct gaagcagatt      900

gccaaggaaa ttggcgtgaa tccggaggat atcaagggat accggatcac taagtccggc      960

acgccagagt tcaccgagtt caagctgtac cacgatctga agtcggtgct ctttgaccag     1020

tccatcctgg aaaacgaaga tgtgctggac cagattgctg agatcctgac catctaccag     1080

gacaaggact cgattaagtc caagctcacc gagctggaca ttctgctgaa cgaagaagat     1140

aaggagaaca tcgcgcagct caccggttac aatggtaccc accgcttgtc ccttaagtgc     1200

atccgcctgg tgctggagga acagtggtac tcgagccgga accagatgga gatcttcact     1260

cacttgaaca tcaagccgaa aaagattaac ctgactgccg ccaacaagat acccaaggcc     1320

atgatcgacg agtttatcct ctcaccggtg gtcaagcgca ccttcattca atctatcaac     1380

gtgatcaaca aggtcatcga gaagtacggc attcctgagg atatcatcat cgagctggct     1440

cgggagaaca actcagacga taggaagaag ttcattaaca acctccagaa aaagaacgag     1500

gccactcgca agcggattaa tgagatcatc ggtcagaccg ggaaccagaa cgccaagcgg     1560

atcgtggaaa agattcggct ccacgaccaa caggagggaa agtgtctgta ctcgctggag     1620

tcgattgcac tgatggacct cctgaacaac ccacagaact acgaagtcga tcacataatc     1680

ccccgcagcg tggcattcga caactccatc cataacaagg tcctcgtgaa gcagatcgag     1740

aatagcaaga aggggaaccg gactccgtac cagtacctga actcctccga cgccaagctg     1800

tcatacaatc agttcaaaca gcacattctc aacctgtcca agtcaaagga caggatctcc     1860

aagaagaaga aggactacct tctcgaggaa cgggatatca ataagttcga ggtgcagaag     1920

gagtttatca atagaaacct ggtggacact cgctatgcca cccgcgaact gaccagctac     1980

ctgaaggcgt acttctccgc caacaacatg gacgtgaagg tcaaaactat taacggcagc     2040

ttcaccaacc atctgcgcaa ggtctggagg ttcgacaagt accgcaacca cggttacaag     2100

caccacgcgg aagatgcgct gattatcgcc aacgctgact tcctgttcaa ggaaaacaag     2160

aagctcaagg ccgtgaactc agtgctcgag aagcctgaaa tcgagactaa gcagctggac     2220

atccaggtcg attcggaaga taactactcc gaaatgttca tcatccctaa gcaagtgcag     2280

gacatcaagg acttcaggaa tttcaagtac agccatcgcg tggacaagaa gccaaacaga     2340

cagctgatca acgatacact gtattccacc cggaagaagg acaactccac ctacatcgtc     2400

caaaccatta aggacatcta cgcaaaggac aacaccacgc ttaagaagca gttcgacaag     2460

agccccgaaa agttcctcat gtaccagcac gaccccagaa ccttcgagaa gcttgaagtg     2520

atcatgaagc agtacgccaa cgaaaagaac ccactggcta agtaccacga ggaaaccggc     2580

gaatacctga ccaagtactc caaaaagaac aacggaccga tcgtcaagtc cctgaagtac     2640

attgggaaca agctcggctc gcacctcgat gtgacccacc agttcaagtc ctcgaccaaa     2700

aagctcgtga agctgtccat caagccgtac cggttcgacg tgtacctgac tgacaaggga     2760

tataagttca tcaccatttc ctacctcgac gtgttgaaga aggataacta ctactacatt     2820

ccggaacaga agtacgacaa gctcaagctc ggaaaggcca tcgacaaaaa tgcgaagttc     2880

atcgcgagct tctacaagaa tgacttgatc aagctggatg gcgaaatcta caagatcatc     2940

ggggtcaact ccgatacccg caacatgatt gagctggatc tgcccgacat tcggtacaag     3000

gaatactgcg agctgaacaa catcaaggga gaaccgcgga tcaagaaaac catcggaaag     3060

aaagtgaaca gcatcgagaa actgactact gacgtcctgg gaaacgtgtt caccaacaca     3120

caatacacca aaccccagct gctgtttaag cgcgggaac                            3159


<210>  17
<211>  3162
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized P2H12Cas9 polynucleotide

<400>  17
atgaaccaaa agttcattct ggggctcgat atcggcatca cctccgtggg atatggtctg       60

atcgactacg agactaagaa catcatcgac gctggagtgc gactgttccc ggaagcgaac      120

gtggagaaca acgaaggccg cagatccaag cgcgggtcca gaaggctcaa gaggcggagg      180

atccatagac tcgagagagt gaagaagctc cttgaagatt acaatctgtt ggaccagtca      240

cagattcccc aaagcaccaa cccgtacgcc atcagagtga agggcctgtc agaagcactg      300

tcgaaagatg aactggtcat tgccctgctg catattgcca aacggcgcgg aatccataac      360

atcaacgtgt cgagcgaaga tgaggacgcg tccaacgaac tgtcaaccaa ggaacagatc      420

aaccggaaca acaaactgct gaaggacaaa tacgtctgcg aggtgcagct tcaacggctg      480

aaagagggac agatcagggg agagaaaaac cggttcaaga ccaccgacat ccttaaggag      540

atcgaccaac tcctgaaagt gcagaaggac tatcacaacc tcgacattga ttttatcaac      600

cagtacaagg agattgtgga aactcggagg gagtacttcg agggacctgg aaagggatcc      660

ccttatggct gggaagggga ccccaaggct tggtacgaaa ccctgatggg ccattgcact      720

tactttccgg atgaactccg gtccgtgaag tacgcttact ctgccgacct gttcaatgca      780

ctcaacgacc ttaacaactt ggtgatccaa cgcgatggtc tttccaagtt ggagtaccac      840

gaaaagtacc acatcatcga gaacgtgttc aagcagaaaa agaagccaac tctgaagcag      900

attgccaacg aaattaacgt gaaccccgag gatatcaagg gataccggat taccaagtcc      960

ggcaaaccag agttcacctc attcaagctg tttcacgatc tgaagaaggt cgtgaaggac     1020

cacgccatcc tggatgacat tgatcttctg aaccagattg ctgagatcct gaccatctac     1080

caggacaagg actcgattgt ggccgaactg ggacagctcg agtacctgat gtccgaagcc     1140

gataagcagt ccatcagcga actcaccggt tacaccggta cccactcctt gtcccttaag     1200

tgcatgaaca tgatcattga cgaactgtgg cactccagca tgaaccagat ggaggtgttc     1260

acctacttga acatgcgccc gaagaagtac gagctgaagg gctaccagcg catacccacg     1320

gacatgatcg acgacgccat cctctcaccg gtggtcaagc gcaccttcat tcaatctatc     1380

aacgtgatca acaaggtcat cgagaagtac ggcattcctg aggatatcat catcgagctg     1440

gctcgggaga acaactcaga cgataggaag aagttcatta acaacctcca gaaaaagaac     1500

gaggccactc gcaagcggat taatgagatc atcggtcaga ccgggaacca gaacgccaag     1560

cggatcgtgg aaaagattcg gctccacgac caacaggagg gaaagtgtct gtactcgctg     1620

gagtccattc ccctggagga cctcctgaac aacccaaacc actacgaggt cgatcacata     1680

atcccccgca gcgtgtcatt cgacaactcc taccataaca aggtcctcgt gaagcagtcg     1740

gagaatagca agaagtcgaa cctgactccg taccagtact tcaactccgg caaatccaag     1800

ctgtcctaca atcagttcaa acagcacatt ctcaacctgt ccaagagcca ggacaggatt     1860

tcgaagaaga agaaggaata ccttctcgag gaacgggata tcaataagtt cgaggtgcag     1920

aaggagttta tcaatagaaa cctggtggac actcgctatg ccacccgcga actgaccaac     1980

tacctgaagg cgtacttctc cgccaacaac atgaacgtga aggtcaaaac tattaacggc     2040

agcttcaccg actatctgcg caaggtctgg aagttcaaga aggaacgcaa ccacggttac     2100

aagcaccacg cggaagatgc gctgattatc gccaacgctg acttcctgtt caaggaaaac     2160

aagaagctca aggccgtgaa ctcagtgctc gagaagcctg aaatcgagac taagcagctg     2220

gacatccagg tcgattcgga agataactac tccgaaatgt tcatcatccc taagcaagtg     2280

caggacatca aggacttcag gaatttcaag tacagccatc gcgtggacaa gaagccaaac     2340

agacagctga tcaacgatac actgtattcc acccggaaga aggacaactc cacctacatc     2400

gtccaaacca ttaaggacat ctacgcaaag gacaacacca cgcttaagaa gcagttcgac     2460

aagagccccg aaaagttcct catgtaccag cacgacccca gaaccttcga gaagcttgaa     2520

gtgatcatga agcagtacgc caacgaaaag aacccactgg ctaagtacca cgaggaaacc     2580

ggcgaatacc tgaccaagta ctccaaaaag aacaacggac cgatcgtcaa gtccctgaag     2640

tacattggga acaagctcgg ctcgcacctc gatgtgaccc accagttcaa gtcctcgacc     2700

aaaaagctcg tgaagctgtc catcaagccg taccggttcg acgtgtacct gactgacaag     2760

ggatataagt tcatcaccat ttcctacctc gacgtgttga agaaggataa ctactactac     2820

attccggaac agaagtacga caagctcaag ctcggaaagg ccatcgacaa aaatgcgaag     2880

ttcatcgcga gcttctacaa gaatgacttg atcaagctgg atggcgaaat ctacaagatc     2940

atcggggtca actccgatac ccgcaacatg attgagctgg atctgcccga cattcggtac     3000

aaggaatact gcgagctgaa caacatcaag ggagaaccgc ggatcaagaa aaccatcgga     3060

aagaaagtga acagcatcga gaaactgact actgacgtcc tgggaaacgt gttcaccaac     3120

acacaataca ccaaacccca gctgctgttt aagcgcggga ac                        3162


<210>  18
<211>  3162
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized SluCas9-1 polynucleotide

<400>  18
atgaaccaaa agttcattct ggggctcgat atcggcatca cctccgtggg atatggtctg       60

atcgactacg agactaagaa catcatcgac gctggagtgc gactgttccc ggaagcgaac      120

gtggagaaca acgaaggccg cagatccaag cgcgggtcca gaaggctcaa gaggcggagg      180

atccatagac tcgaaagagt gaagaagctc cttgaagatt acaatctgtt ggaccagagc      240

cagattcccc aaagcaccaa cccgtacgcc atcagagtga agggcctgtc cgaagccctg      300

tcgaaagatg aactggtcat tgccctgctg catattgcca aacggcgcgg aatccataag      360

atcgacgtga tagactccaa cgatgacgtg ggcaacgaac tgtcaaccaa ggagcagctg      420

aacaagaact cgaaactgct gaaggacaag ttcgtctgcc aaattcaact ggaacggatg      480

aacgagggac aagtcagggg agagaaaaac cggttcaaga ccgcggacat catcaaggag      540

atcatccaac tcctgaatgt gcagaagaac tttcaccagc tggatgaaaa cttcattaac      600

aagtacattg aactggtgga aatgcggagg gagtacttcg agggacctgg aaagggatcc      660

ccttacggct gggaagggga ccccaaggct tggtacgaaa cgctcatggg ccattgcact      720

tactttccgg acgaactccg gtccgtgaag tacgcatact ctgccgatct gttcaatgca      780

ctcaacgacc ttaacaactt ggtgatccag cgcgatggcc tgtccaagtt ggaataccac      840

gaaaagtatc acatcatcga gaacgtgttc aagcagaaaa agaagccaac tctgaagcag      900

attgccaacg aaattaacgt gaaccccgag gatatcaagg gataccggat cactaagtcc      960

ggcaaaccac agttcaccga gttcaagctg taccacgatc tgaagtcggt gctctttgac     1020

cagtccatcc tggaaaacga agatgtgctg gaccagattg ctgagatcct gaccatctac     1080

caggacaagg actcgattaa gagcaagctc acggagctgg acattctgct gaacgaagag     1140

gataaggaga acatcgcgca gctcactggt tacaccggta cccaccgctt gtcccttaag     1200

tgcatccggc tggtcctcga ggaacaatgg tactccagcc ggaaccagat ggagatcttc     1260

acgcacttga acatcaagcc gaagaagatt aacctgaccg ctgcgaacaa gatacccaag     1320

gccatgatcg acgagtttat cctctcaccg gtggtcaagc gcaccttcgg acaagccatc     1380

aacctcatca acaagattat cgagaagtac ggcgtgcctg aggatatcat catcgagctg     1440

gctcgggaga acaactcaaa ggataagcag aagttcatta acgagatgca gaaaaagaac     1500

gagaacactc gcaagcggat taatgagatc atcggtaaat acgggaacca gaacgccaag     1560

cggcttgtgg aaaagattcg gctccacgac gagcaggagg gaaagtgtct gtactcgctg     1620

gagagcattc ccctggagga cctcctgaac aacccaaacc actacgaagt ggatcacata     1680

atcccccgca gcgtgtcatt cgacaattcc taccataaca aggtcctcgt gaagcagtcc     1740

gagaatagca agaagtccaa cctgactccg taccagtact tcaactccgg caaatccaag     1800

ctgtcctaca accagttcaa acagcacatc ctcaacctgt caaagagcca ggacaggatc     1860

tcgaagaaga agaaggaata ccttctcgag gaacgggata tcaataagtt cgaggtgcag     1920

aaggagttta tcaatagaaa cctggtggac actcgctatg ccacccgcga actgaccaac     1980

tacctgaagg cgtacttctc cgccaacaac atgaacgtga aggtcaaaac tattaacggc     2040

agcttcaccg actatctgcg caaggtctgg aagttcaaga aggaacgcaa ccacggttac     2100

aagcaccacg cggaagatgc gctgattatc gccaacgctg acttcctgtt caaggaaaac     2160

aagaagctca aggccgtgaa ctcagtgctc gagaagcctg aaatcgagac taagcagctg     2220

gacatccagg tcgattcgga agataactac tccgaaatgt tcatcatccc taagcaagtg     2280

caggacatca aggacttcag gaatttcaag tacagccatc gcgtggacaa gaagccaaac     2340

agacagctga tcaacgatac actgtattcc acccggaaga aggacaactc cacctacatc     2400

gtccaaacca ttaaggacat ctacgcaaag gacaacacca cgcttaagaa gcagttcgac     2460

aagagccccg aaaagttcct catgtaccag cacgacccca gaaccttcga gaagcttgaa     2520

gtgatcatga agcagtacgc caacgaaaag aacccactgg ctaagtacca cgaggaaacc     2580

ggcgaatacc tgaccaagta ctccaaaaag aacaacggac cgatcgtcaa gtccctgaag     2640

tacattggga acaagctcgg ctcgcacctc gatgtgaccc accagttcaa gtcctcgacc     2700

aaaaagctcg tgaagctgtc catcaagccg taccggttcg acgtgtacct gactgacaag     2760

ggatataagt tcatcaccat ttcctacctc gacgtgttga agaaggataa ctactactac     2820

attccggaac agaagtacga caagctcaag ctcggaaagg ccatcgacaa aaatgcgaag     2880

ttcatcgcga gcttctacaa gaatgacttg atcaagctgg atggcgaaat ctacaagatc     2940

atcggggtca actccgatac ccgcaacatg attgagctgg atctgcccga cattcggtac     3000

aaggaatact gcgagctgaa caacatcaag ggagaaccgc ggatcaagaa aaccatcgga     3060

aagaaagtga acagcatcga gaaactgact actgacgtcc tgggaaacgt gttcaccaac     3120

acacaataca ccaaacccca gctgctgttt aagcgcggga ac                        3162


<210>  19
<211>  3162
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Codon-optimized SluCas9-2 polynucleotide

<400>  19
atgaaccaga agttcatcct gggcctcgac atcggcatca cctctgttgg ctacggcctg       60

atcgactacg agacaaagaa catcatcgat gccggcgtgc ggctgttccc tgaggccaac      120

gtggaaaaca acgagggccg cagaagcaag agaggcagca gaaggctgaa gcggcggaga      180

atccaccggc tggaaagagt gaagaagctg ctcgaggact acaacctgct ggaccagtct      240

cagatccctc agagcacaaa cccctacgcc atcagagtga agggcctgtc tgaggccctg      300

agcaaggacg agctggttat cgccctgctg cacattgcca agcggagagg catccacaag      360

atcgacgtga tcgacagcaa cgacgacgtg ggcaatgagc tgagcaccaa agagcagctg      420

aacaagaaca gcaagctgct gaaggacaag ttcgtgtgcc agattcagct ggaacggatg      480

aatgagggcc aagtgcgggg cgagaagaac agattcaaga ccgccgacat catcaaagag      540

atcatccagc tgctcaacgt gcagaagaac ttccaccagc tggacgagaa cttcatcaac      600

aagtacatcg agctggtcga gatgcggcgc gagtactttg aaggccctgg aaagggcagc      660

ccttatggct gggaaggcga tcccaaggct tggtacgaga cactgatggg ccactgcacc      720

tactttcccg acgagctgag aagcgtgaag tacgcctaca gcgccgacct gttcaacgcc      780

ctgaacgacc tgaacaacct cgtgatccag agagatggcc tgtccaagct ggaataccac      840

gagaagtacc acatcattga gaacgtgttc aagcagaaga agaagcccac actgaagcag      900

atcgccaacg agatcaacgt gaaccccgag gacatcaagg gctacagaat caccaagagc      960

ggcaagcccc agttcaccga gttcaagctg taccacgatc tgaagtccgt gctgttcgac     1020

cagagcatcc tggaaaacga ggacgtgctg gatcagatcg ccgagatcct gaccatctac     1080

caggacaagg acagcatcaa gagcaagctg accgagctgg acatcctgct gaacgaagag     1140

gacaaagaga atatcgccca gctgaccggc tacaccggca cacatagact gagcctgaag     1200

tgcatccggc tggtgctgga agaacagtgg tactccagcc ggaaccagat ggaaatcttc     1260

acccacctga acatcaagcc caagaagatc aacctgaccg ccgccaacaa gatccccaag     1320

gccatgatcg acgagttcat tctgagcccc gtggtcaaga gaaccttcgg ccaggccatc     1380

aatctgatca acaagattat cgagaagtat ggcgtgcccg aggatatcat catcgaactg     1440

gccagagaga acaacagcaa ggacaagcaa aagttcatca acgagatgca gaaaaagaac     1500

gagaacaccc ggaagcggat caacgaaatc atcgggaagt acggcaacca gaacgccaag     1560

agactggtgg aaaagatccg gctgcacgac gagcaagagg gcaagtgtct gtacagcctg     1620

gaatctatcc ctctcgagga tctgctgaac aatcccaacc actacgaggt ggaccacatt     1680

atccccagaa gcgtgtcctt cgacaacagc taccacaaca aggtgctggt caagcagagc     1740

gagaactcca agaagtccaa tctgacccct taccagtact tcaacagcgg caagtctaag     1800

ctgagctaca accagtttaa gcagcacatc ctgaacctca gcaagagcca ggaccggatc     1860

agcaagaaga agaaagagta cctgctcgaa gagagggaca ttaacaagtt cgaggtgcag     1920

aaagagttta tcaaccggaa cctggtggac accagatacg ccaccagaga gctgaccaac     1980

tacctgaagg cctacttcag cgccaacaac atgaacgtga aagtcaagac catcaacggc     2040

agcttcaccg actacctgcg gaaagtgtgg aagtttaaga aagagcggaa ccacggctac     2100

aagcaccacg ccgaagatgc cctgattatc gccaatgccg acttcctgtt caaagagaac     2160

aagaaactga aggccgtgaa cagcgtgctg gaaaagcccg agatcgagac aaaacagctc     2220

gacatccagg tggacagcga ggacaactac agcgagatgt tcatcatccc caaacaggtg     2280

caggatatca aggacttccg gaacttcaag tacagccacc gcgtggacaa gaagcctaac     2340

cggcagctga tcaatgacac cctgtacagc acccgcaaga aggacaacag cacctacatc     2400

gtgcagacga tcaaggacat ctacgccaag gacaatacga ccctgaagaa gcagttcgac     2460

aagagccccg agaagttcct gatgtaccag cacgacccca ggaccttcga gaagctggaa     2520

gtgatcatga agcagtacgc taatgagaag aacccgctgg ccaagtacca cgaggaaacc     2580

ggcgagtacc tgaccaagta ctctaagaag aacaacggcc ccatcgtgaa gtccctgaag     2640

tatatcggca acaagctggg cagccacctg gacgtgacac accagttcaa gagcagcacc     2700

aagaagctgg tcaaactgtc catcaagcca taccgcttcg acgtgtacct gacagacaag     2760

gggtacaagt ttatcaccat cagctacctc gacgtgctga agaaggataa ctactactac     2820

atccccgagc agaagtacga caagctgaag ctgggaaaag ccatcgacaa gaatgccaag     2880

ttcattgcca gcttctacaa gaacgacctc atcaagctgg acggcgagat ctacaagatc     2940

atcggcgtga actccgacac acggaacatg attgagctgg acctgcctga catccggtac     3000

aaagagtact gcgaactgaa caatatcaag ggcgagcccc ggatcaaaaa gacgatcggc     3060

aagaaagtga acagcattga gaagctgacc accgatgtgc tgggcaatgt gttcaccaac     3120

acacagtaca ccaagcctca gctgctgttc aagcggggca at                        3162


<210>  20
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  5 prime UTR

<400>  20
aggaaataag agagaaaaga agagtaagaa gaaatataag agccacc                     47


<210>  21
<211>  114
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  3 prime UTR

<400>  21
gcggccgctt aattaagctg ccttctgcgg ggcttgcctt ctggccatgc ccttcttctc       60

tcccttgcac ctgtacctct tggtctttga ataaagcctg agtaggaagt ctag            114


<210>  22
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  nucleoplasmin NLS, nucleotide

<400>  22
aaacggcctg ccgcgaccaa gaaagccggc caggccaaga agaagaag                    48


<210>  23
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  nucleoplasmin NLS, amino acid

<400>  23

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  24
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  SV40 NLS, nucleotide

<400>  24
cctaagaaga agcgcaaggt c                                                 21


<210>  25
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  SV40 NLS, amino acid

<400>  25

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  26
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  gly-ser linker 1, nucleotide

<400>  26
ggtggtggcg gatcgggggg gggcggtagc gggggggggg gctctggctc g                51


<210>  27
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  gly-ser linker 1, amino acid

<400>  27

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Ser 
    


<210>  28
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  gly-ser linker 2, nucleotide

<400>  28
gggggctccg gaggatccgg tggcagcggc ccc                                    33


<210>  29
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  gly-ser linker 2, amino acid

<400>  29

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Pro 
1               5                   10      


<210>  30
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  gly-ser linker 3, nucleotide

<400>  30
gggggcggag gaggctca                                                     18


<210>  31
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  gly-ser linker 3, amino acid

<400>  31

Gly Gly Gly Gly Gly Ser 
1               5       


<210>  32
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  6xHis tag, nucleotide

<400>  32
catcaccatc accaccat                                                     18


<210>  33
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  6xHis tag, amino acid

<400>  33

His His His His His His 
1               5       


<210>  34
<211>  3530
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  GST3, nucleotide

<400>  34
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg aaacggcctg       60

ccgcgaccaa gaaagccggc caggccaaga agaagaaggg tggtggcgga tcgggggggg      120

gcggtagcgg gggggggggc tctggctcga tgaaccagaa gttcattctc ggtctggaca      180

ttggcattac tagcgtggga tacggcttga ttgactacga gactaagaac atcatcgatg      240

ccggcgtccg cctgttcccg gaagccaacg tggagaacaa tgagggccgg aggtcgaaga      300

gaggctcccg ccgcctgaag cggcggcgaa tccaccggct ggagagagtg aagctgctgc      360

tcaccgaata cgacctgatc aacaaagaac agatcccgac ctccaacaac ccgtaccaga      420

tcagagtgaa gggtctgtca gaaatcctgt ccaaggacga actggcaatc gccctgctgc      480

acctggccaa gcggcgcgga atccacaacg tggatgtggc tgccgacaag gaagaaaccg      540

cttccgactc cctgagcact aaggaccaga tcaacaagaa cgccaagttc ttggagtccc      600

gctacgtgtg cgagctgcag aaggaacggc tggaaaacga aggtcacgtg cgcggagtgg      660

aaaaccggtt cctgacaaag gacattgtgc gcgaagcgaa gaagatcatt gatacccaaa      720

tgcagtacta ccctgaaatc gacgagactt tcaaggaaaa gtacatttcc ctggtggaaa      780

cccggcggga atacttcgaa ggccccggac agggatcgcc gttcggatgg aacggggacc      840

tcaagaagtg gtacgagatg ctgatggggc actgtaccta ctttccgcaa gaactgcgct      900

ccgtgaagta cgcgtactcc gcggatctct tcaacgcgtt gaatgacctg aacaacctga      960

tcattcagag agacaattcc gaaaagctcg agtaccacga gaagtatcac atcatcgaga     1020

atgtgttcaa gcagaagaag aaaccgaccc tcaagcaaat cgccaaggag attggcgtca     1080

acccagagga catcaaggga tatcggatta ccaagagcgg cactcccgag tttacctctt     1140

tcaagctgtt tcatgatctg aagaaagtcg tgaaggacca tgccattctc gacgacattg     1200

atctcctgaa tcagatcgca gagatcctga ctatctacca agacaaggac tcgattgtgg     1260

cagagctggg tcagctcgaa tacctgatgt ccgaggccga caagcagtcc atctccgaac     1320

tgacagggta cacggggact catagcctgt cgctgaagtg catgaacatg atcattgatg     1380

aactgtggca cagctccatg aaccaaatgg aagtgtttac ctacctcaac atgcgcccta     1440

agaagtacga actgaaaggc taccagcgca tccccaccga catgatcgac gacgcgatct     1500

tgtcccctgt ggtcaagagg accttcattc aatccatcaa cgtgatcaac aaggtcatcg     1560

aaaagtacgg tattccagaa gatatcatca ttgagctcgc tcgggagaac aactcggatg     1620

accggaagaa gttcatcaac aatcttcaga agaagaacga agcgactcgg aaacggatca     1680

acgagatcat cggacagacc ggaaaccaga acgccaaacg gattgtcgaa aagattagac     1740

tgcacgacca gcaggaaggg aagtgcctgt actcactcga gtcaataccg ctcgaggacc     1800

tgttgaacaa ccctaaccac tatgaagtgg accacatcat ccctcggtcc gtgagcttcg     1860

acaactcgta ccacaacaaa gtgctcgtga agcagtccga aaactccaag aaatccaacc     1920

tgaccccgta ccaatacttc aattcgggaa agtcgaagct gtcgtacaac cagttcaaac     1980

aacacatact caaccttagc aaaagccagg atcgcatttc caagaagaag aaggaatacc     2040

tcctcgagga aagggacatc aacaagttcg aagtgcagaa agagttcatc aatcgcaact     2100

tggtggatac cagatatgcc acccgggaac tcaccagcta tctcaaggcc tacttttccg     2160

ccaacaacat ggacgtgaag gtcaagacca tcaacgggtc cttcactaac catctgagaa     2220

aggtctggcg gtttgacaag taccgcaacc acggatacaa gcaccacgct gaagacgctc     2280

tgatcatcgc caatgccgac ttcctgttca aggaaaacaa gaagctgcag aacacgaaca     2340

agattctgga aaagcctacc attgagaaca acactaagaa ggtcaccgtg gagaaggaag     2400

aggactacaa caacgtgttc gaaactccta aactggtgga ggatatcaag caataccgcg     2460

actacaagtt ctcacaccgg gtggacaaga aaccgaatag acagctgatc aacgacacgt     2520

tgtattccac ccggatgaag gatgagcatg actacattgt gcagactatc accgatatct     2580

acggaaaaga taacactaac ctgaagaaac aattcaacaa gaacccagag aagttcctga     2640

tgtaccagaa cgaccccaag acctttgaga agctttccat catcatgaag cagtactccg     2700

acgagaagaa cccgctggcc aagtactacg aagaaaccgg agaatacctg accaagtaca     2760

gcaagaagaa caacggtccc attgtcaaga agatcaagct gctcggcaac aaggtcggaa     2820

accacctcga cgtgacaaac aagtacgaga actcgactaa gaagcttgtg aagctgtcaa     2880

tcaagaacta tagattcgac gtgtacttga ccgaaaaggg atacaagttc gtgaccatag     2940

cctatctgaa cgtgttcaag aaagataact actactacat ccccaaggac aagtaccagg     3000

agctcaaaga aaagaagaag atcaaagaca ccgaccagtt cattgcctcc ttctacaaga     3060

acgacctgat caaactgaac ggcgacctct acaagatcat tggagtgaac agcgatgaca     3120

ggaacatcat tgagctggac tactacgaca tcaagtacaa ggactactgc gagatcaaca     3180

acatcaaggg cgaaccccgg atcaagaaaa ctattggaaa gaaaaccgag tccattgaga     3240

agttcaccac tgacgtgctg ggaaaccttt acctccactc caccgagaag gcaccacaac     3300

tgatcttcaa gcgcggcctg gggggctccg gaggatccgg tggcagcggc cccccgaaga     3360

agaagcgcaa agtcgggggc ggaggaggct cacatcacca tcaccaccat taataagcgg     3420

ccgcttaatt aagctgcctt ctgcggggct tgccttctgg ccatgccctt cttctctccc     3480

ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaagtctag                3530


<210>  35
<211>  1121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  GST3, amino acid

<400>  35

Met Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys 
1               5                   10                  15      


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25                  30          


Gly Ser Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile Thr 
        35                  40                  45              


Ser Val Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile Asp 
    50                  55                  60                  


Ala Gly Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu Gly 
65                  70                  75                  80  


Arg Arg Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile His 
                85                  90                  95      


Arg Leu Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile Asn 
            100                 105                 110         


Lys Glu Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val Lys 
        115                 120                 125             


Gly Leu Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu Leu 
    130                 135                 140                 


His Leu Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala Asp 
145                 150                 155                 160 


Lys Glu Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile Asn 
                165                 170                 175     


Lys Asn Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln Lys 
            180                 185                 190         


Glu Arg Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg Phe 
        195                 200                 205             


Leu Thr Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr Gln 
    210                 215                 220                 


Met Gln Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr Ile 
225                 230                 235                 240 


Ser Leu Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln Gly 
                245                 250                 255     


Ser Pro Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met Leu 
            260                 265                 270         


Met Gly His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys Tyr 
        275                 280                 285             


Ala Tyr Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn Leu 
    290                 295                 300                 


Ile Ile Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His Glu Lys Tyr 
305                 310                 315                 320 


His Ile Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu Lys 
                325                 330                 335     


Gln Ile Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile Lys Gly Tyr 
            340                 345                 350         


Arg Ile Thr Lys Ser Gly Thr Pro Glu Phe Thr Ser Phe Lys Leu Phe 
        355                 360                 365             


His Asp Leu Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp Ile 
    370                 375                 380                 


Asp Leu Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp Lys 
385                 390                 395                 400 


Asp Ser Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser Glu 
                405                 410                 415     


Ala Asp Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr His 
            420                 425                 430         


Ser Leu Ser Leu Lys Cys Met Asn Met Ile Ile Asp Glu Leu Trp His 
        435                 440                 445             


Ser Ser Met Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg Pro 
    450                 455                 460                 


Lys Lys Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met Ile 
465                 470                 475                 480 


Asp Asp Ala Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln Ser 
                485                 490                 495     


Ile Asn Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu Asp 
            500                 505                 510         


Ile Ile Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys Lys 
        515                 520                 525             


Phe Ile Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg Ile 
    530                 535                 540                 


Asn Glu Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile Val 
545                 550                 555                 560 


Glu Lys Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr Ser 
                565                 570                 575     


Leu Glu Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Asn His Tyr 
            580                 585                 590         


Glu Val Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr 
        595                 600                 605             


His Asn Lys Val Leu Val Lys Gln Ser Glu Asn Ser Lys Lys Ser Asn 
    610                 615                 620                 


Leu Thr Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser Tyr 
625                 630                 635                 640 


Asn Gln Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser Gln Asp Arg 
                645                 650                 655     


Ile Ser Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile Asn 
            660                 665                 670         


Lys Phe Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp Thr 
        675                 680                 685             


Arg Tyr Ala Thr Arg Glu Leu Thr Ser Tyr Leu Lys Ala Tyr Phe Ser 
    690                 695                 700                 


Ala Asn Asn Met Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe Thr 
705                 710                 715                 720 


Asn His Leu Arg Lys Val Trp Arg Phe Asp Lys Tyr Arg Asn His Gly 
                725                 730                 735     


Tyr Lys His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp Phe 
            740                 745                 750         


Leu Phe Lys Glu Asn Lys Lys Leu Gln Asn Thr Asn Lys Ile Leu Glu 
        755                 760                 765             


Lys Pro Thr Ile Glu Asn Asn Thr Lys Lys Val Thr Val Glu Lys Glu 
    770                 775                 780                 


Glu Asp Tyr Asn Asn Val Phe Glu Thr Pro Lys Leu Val Glu Asp Ile 
785                 790                 795                 800 


Lys Gln Tyr Arg Asp Tyr Lys Phe Ser His Arg Val Asp Lys Lys Pro 
                805                 810                 815     


Asn Arg Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Met Lys Asp 
            820                 825                 830         


Glu His Asp Tyr Ile Val Gln Thr Ile Thr Asp Ile Tyr Gly Lys Asp 
        835                 840                 845             


Asn Thr Asn Leu Lys Lys Gln Phe Asn Lys Asn Pro Glu Lys Phe Leu 
    850                 855                 860                 


Met Tyr Gln Asn Asp Pro Lys Thr Phe Glu Lys Leu Ser Ile Ile Met 
865                 870                 875                 880 


Lys Gln Tyr Ser Asp Glu Lys Asn Pro Leu Ala Lys Tyr Tyr Glu Glu 
                885                 890                 895     


Thr Gly Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro Ile 
            900                 905                 910         


Val Lys Lys Ile Lys Leu Leu Gly Asn Lys Val Gly Asn His Leu Asp 
        915                 920                 925             


Val Thr Asn Lys Tyr Glu Asn Ser Thr Lys Lys Leu Val Lys Leu Ser 
    930                 935                 940                 


Ile Lys Asn Tyr Arg Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr Lys 
945                 950                 955                 960 


Phe Val Thr Ile Ala Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr Tyr 
                965                 970                 975     


Tyr Ile Pro Lys Asp Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys Ile 
            980                 985                 990         


Lys Asp Thr Asp Gln Phe Ile Ala  Ser Phe Tyr Lys Asn  Asp Leu Ile 
        995                 1000                 1005             


Lys Leu  Asn Gly Asp Leu Tyr  Lys Ile Ile Gly Val  Asn Ser Asp 
    1010                 1015                 1020             


Asp Arg  Asn Ile Ile Glu Leu  Asp Tyr Tyr Asp Ile  Lys Tyr Lys 
    1025                 1030                 1035             


Asp Tyr  Cys Glu Ile Asn Asn  Ile Lys Gly Glu Pro  Arg Ile Lys 
    1040                 1045                 1050             


Lys Thr  Ile Gly Lys Lys Thr  Glu Ser Ile Glu Lys  Phe Thr Thr 
    1055                 1060                 1065             


Asp Val  Leu Gly Asn Leu Tyr  Leu His Ser Thr Glu  Lys Ala Pro 
    1070                 1075                 1080             


Gln Leu  Ile Phe Lys Arg Gly  Leu Gly Gly Ser Gly  Gly Ser Gly 
    1085                 1090                 1095             


Gly Ser  Gly Pro Pro Lys Lys  Lys Arg Lys Val Gly  Gly Gly Gly 
    1100                 1105                 1110             


Gly Ser  His His His His His  His 
    1115                 1120     


<210>  36
<211>  3533
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  GST3-K, nucleotide

<400>  36
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcgaaacggc       60

ctgccgcgac caagaaagcc ggccaggcca agaagaagaa gggtggtggc ggatcggggg      120

ggggcggtag cggggggggg ggctctggct cgatgaacca gaagttcatt ctcggtctgg      180

acattggcat tactagcgtg ggatacggct tgattgacta cgagactaag aacatcatcg      240

atgccggcgt ccgcctgttc ccggaagcca acgtggagaa caatgagggc cggaggtcga      300

agagaggctc ccgccgcctg aagcggcggc gaatccaccg gctggagaga gtgaagctgc      360

tgctcaccga atacgacctg atcaacaaag aacagatccc gacctccaac aacccgtacc      420

agatcagagt gaagggtctg tcagaaatcc tgtccaagga cgaactggca atcgccctgc      480

tgcacctggc caagcggcgc ggaatccaca acgtggatgt ggctgccgac aaggaagaaa      540

ccgcttccga ctccctgagc actaaggacc agatcaacaa gaacgccaag ttcttggagt      600

cccgctacgt gtgcgagctg cagaaggaac ggctggaaaa cgaaggtcac gtgcgcggag      660

tggaaaaccg gttcctgaca aaggacattg tgcgcgaagc gaagaagatc attgataccc      720

aaatgcagta ctaccctgaa atcgacgaga ctttcaagga aaagtacatt tccctggtgg      780

aaacccggcg ggaatacttc gaaggccccg gacagggatc gccgttcgga tggaacgggg      840

acctcaagaa gtggtacgag atgctgatgg ggcactgtac ctactttccg caagaactgc      900

gctccgtgaa gtacgcgtac tccgcggatc tcttcaacgc gttgaatgac ctgaacaacc      960

tgatcattca gagagacaat tccgaaaagc tcgagtacca cgagaagtat cacatcatcg     1020

agaatgtgtt caagcagaag aagaaaccga ccctcaagca aatcgccaag gagattggcg     1080

tcaacccaga ggacatcaag ggatatcgga ttaccaagag cggcactccc gagtttacct     1140

ctttcaagct gtttcatgat ctgaagaaag tcgtgaagga ccatgccatt ctcgacgaca     1200

ttgatctcct gaatcagatc gcagagatcc tgactatcta ccaagacaag gactcgattg     1260

tggcagagct gggtcagctc gaatacctga tgtccgaggc cgacaagcag tccatctccg     1320

aactgacagg gtacacgggg actcatagcc tgtcgctgaa gtgcatgaac atgatcattg     1380

atgaactgtg gcacagctcc atgaaccaaa tggaagtgtt tacctacctc aacatgcgcc     1440

ctaagaagta cgaactgaaa ggctaccagc gcatccccac cgacatgatc gacgacgcga     1500

tcttgtcccc tgtggtcaag aggaccttca ttcaatccat caacgtgatc aacaaggtca     1560

tcgaaaagta cggtattcca gaagatatca tcattgagct cgctcgggag aacaactcgg     1620

atgaccggaa gaagttcatc aacaatcttc agaagaagaa cgaagcgact cggaaacgga     1680

tcaacgagat catcggacag accggaaacc agaacgccaa acggattgtc gaaaagatta     1740

gactgcacga ccagcaggaa gggaagtgcc tgtactcact cgagtcaata ccgctcgagg     1800

acctgttgaa caaccctaac cactatgaag tggaccacat catccctcgg tccgtgagct     1860

tcgacaactc gtaccacaac aaagtgctcg tgaagcagtc cgaaaactcc aagaaatcca     1920

acctgacccc gtaccaatac ttcaattcgg gaaagtcgaa gctgtcgtac aaccagttca     1980

aacaacacat actcaacctt agcaaaagcc aggatcgcat ttccaagaag aagaaggaat     2040

acctcctcga ggaaagggac atcaacaagt tcgaagtgca gaaagagttc atcaatcgca     2100

acttggtgga taccagatat gccacccggg aactcaccag ctatctcaag gcctactttt     2160

ccgccaacaa catggacgtg aaggtcaaga ccatcaacgg gtccttcact aaccatctga     2220

gaaaggtctg gcggtttgac aagtaccgca accacggata caagcaccac gctgaagacg     2280

ctctgatcat cgccaatgcc gacttcctgt tcaaggaaaa caagaagctg cagaacacga     2340

acaagattct ggaaaagcct accattgaga acaacactaa gaaggtcacc gtggagaagg     2400

aagaggacta caacaacgtg ttcgaaactc ctaaactggt ggaggatatc aagcaatacc     2460

gcgactacaa gttctcacac cgggtggaca agaaaccgaa tagacagctg atcaacgaca     2520

cgttgtattc cacccggatg aaggatgagc atgactacat tgtgcagact atcaccgata     2580

tctacggaaa agataacact aacctgaaga aacaattcaa caagaaccca gagaagttcc     2640

tgatgtacca gaacgacccc aagacctttg agaagctttc catcatcatg aagcagtact     2700

ccgacgagaa gaacccgctg gccaagtact acgaagaaac cggagaatac ctgaccaagt     2760

acagcaagaa gaacaacggt cccattgtca agaagatcaa gctgctcggc aacaaggtcg     2820

gaaaccacct cgacgtgaca aacaagtacg agaactcgac taagaagctt gtgaagctgt     2880

caatcaagaa ctatagattc gacgtgtact tgaccgaaaa gggatacaag ttcgtgacca     2940

tagcctatct gaacgtgttc aagaaagata actactacta catccccaag gacaagtacc     3000

aggagctcaa agaaaagaag aagatcaaag acaccgacca gttcattgcc tccttctaca     3060

agaacgacct gatcaaactg aacggcgacc tctacaagat cattggagtg aacagcgatg     3120

acaggaacat cattgagctg gactactacg acatcaagta caaggactac tgcgagatca     3180

acaacatcaa gggcgaaccc cggatcaaga aaactattgg aaagaaaacc gagtccattg     3240

agaagttcac cactgacgtg ctgggaaacc tttacctcca ctccaccgag aaggcaccac     3300

aactgatctt caagcgcggc ctggggggct ccggaggatc cggtggcagc ggccccccga     3360

agaagaagcg caaagtcggg ggcggaggag gctcacatca ccatcaccac cattgataag     3420

cggccgctta attaagctgc cttctgcggg gcttgccttc tggccatgcc cttcttctct     3480

cccttgcacc tgtacctctt ggtctttgaa taaagcctga gtaggaagtc tag            3533


<210>  37
<211>  1122
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  GST3-K, amino acid

<400>  37

Met Ala Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys 
1               5                   10                  15      


Lys Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            20                  25                  30          


Ser Gly Ser Met Asn Gln Lys Phe Ile Leu Gly Leu Asp Ile Gly Ile 
        35                  40                  45              


Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr Glu Thr Lys Asn Ile Ile 
    50                  55                  60                  


Asp Ala Gly Val Arg Leu Phe Pro Glu Ala Asn Val Glu Asn Asn Glu 
65                  70                  75                  80  


Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg Leu Lys Arg Arg Arg Ile 
                85                  90                  95      


His Arg Leu Glu Arg Val Lys Leu Leu Leu Thr Glu Tyr Asp Leu Ile 
            100                 105                 110         


Asn Lys Glu Gln Ile Pro Thr Ser Asn Asn Pro Tyr Gln Ile Arg Val 
        115                 120                 125             


Lys Gly Leu Ser Glu Ile Leu Ser Lys Asp Glu Leu Ala Ile Ala Leu 
    130                 135                 140                 


Leu His Leu Ala Lys Arg Arg Gly Ile His Asn Val Asp Val Ala Ala 
145                 150                 155                 160 


Asp Lys Glu Glu Thr Ala Ser Asp Ser Leu Ser Thr Lys Asp Gln Ile 
                165                 170                 175     


Asn Lys Asn Ala Lys Phe Leu Glu Ser Arg Tyr Val Cys Glu Leu Gln 
            180                 185                 190         


Lys Glu Arg Leu Glu Asn Glu Gly His Val Arg Gly Val Glu Asn Arg 
        195                 200                 205             


Phe Leu Thr Lys Asp Ile Val Arg Glu Ala Lys Lys Ile Ile Asp Thr 
    210                 215                 220                 


Gln Met Gln Tyr Tyr Pro Glu Ile Asp Glu Thr Phe Lys Glu Lys Tyr 
225                 230                 235                 240 


Ile Ser Leu Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly Pro Gly Gln 
                245                 250                 255     


Gly Ser Pro Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr Glu Met 
            260                 265                 270         


Leu Met Gly His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser Val Lys 
        275                 280                 285             


Tyr Ala Tyr Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu Asn Asn 
    290                 295                 300                 


Leu Ile Ile Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His Glu Lys 
305                 310                 315                 320 


Tyr His Ile Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro Thr Leu 
                325                 330                 335     


Lys Gln Ile Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile Lys Gly 
            340                 345                 350         


Tyr Arg Ile Thr Lys Ser Gly Thr Pro Glu Phe Thr Ser Phe Lys Leu 
        355                 360                 365             


Phe His Asp Leu Lys Lys Val Val Lys Asp His Ala Ile Leu Asp Asp 
    370                 375                 380                 


Ile Asp Leu Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile Tyr Gln Asp 
385                 390                 395                 400 


Lys Asp Ser Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr Leu Met Ser 
                405                 410                 415     


Glu Ala Asp Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr Thr Gly Thr 
            420                 425                 430         


His Ser Leu Ser Leu Lys Cys Met Asn Met Ile Ile Asp Glu Leu Trp 
        435                 440                 445             


His Ser Ser Met Asn Gln Met Glu Val Phe Thr Tyr Leu Asn Met Arg 
    450                 455                 460                 


Pro Lys Lys Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro Thr Asp Met 
465                 470                 475                 480 


Ile Asp Asp Ala Ile Leu Ser Pro Val Val Lys Arg Thr Phe Ile Gln 
                485                 490                 495     


Ser Ile Asn Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile Pro Glu 
            500                 505                 510         


Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp Arg Lys 
        515                 520                 525             


Lys Phe Ile Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg Lys Arg 
    530                 535                 540                 


Ile Asn Glu Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys Arg Ile 
545                 550                 555                 560 


Val Glu Lys Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys Leu Tyr 
                565                 570                 575     


Ser Leu Glu Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn Pro Asn His 
            580                 585                 590         


Tyr Glu Val Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser 
        595                 600                 605             


Tyr His Asn Lys Val Leu Val Lys Gln Ser Glu Asn Ser Lys Lys Ser 
    610                 615                 620                 


Asn Leu Thr Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser Lys Leu Ser 
625                 630                 635                 640 


Tyr Asn Gln Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser Gln Asp 
                645                 650                 655     


Arg Ile Ser Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu Arg Asp Ile 
            660                 665                 670         


Asn Lys Phe Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu Val Asp 
        675                 680                 685             


Thr Arg Tyr Ala Thr Arg Glu Leu Thr Ser Tyr Leu Lys Ala Tyr Phe 
    690                 695                 700                 


Ser Ala Asn Asn Met Asp Val Lys Val Lys Thr Ile Asn Gly Ser Phe 
705                 710                 715                 720 


Thr Asn His Leu Arg Lys Val Trp Arg Phe Asp Lys Tyr Arg Asn His 
                725                 730                 735     


Gly Tyr Lys His His Ala Glu Asp Ala Leu Ile Ile Ala Asn Ala Asp 
            740                 745                 750         


Phe Leu Phe Lys Glu Asn Lys Lys Leu Gln Asn Thr Asn Lys Ile Leu 
        755                 760                 765             


Glu Lys Pro Thr Ile Glu Asn Asn Thr Lys Lys Val Thr Val Glu Lys 
    770                 775                 780                 


Glu Glu Asp Tyr Asn Asn Val Phe Glu Thr Pro Lys Leu Val Glu Asp 
785                 790                 795                 800 


Ile Lys Gln Tyr Arg Asp Tyr Lys Phe Ser His Arg Val Asp Lys Lys 
                805                 810                 815     


Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg Met Lys 
            820                 825                 830         


Asp Glu His Asp Tyr Ile Val Gln Thr Ile Thr Asp Ile Tyr Gly Lys 
        835                 840                 845             


Asp Asn Thr Asn Leu Lys Lys Gln Phe Asn Lys Asn Pro Glu Lys Phe 
    850                 855                 860                 


Leu Met Tyr Gln Asn Asp Pro Lys Thr Phe Glu Lys Leu Ser Ile Ile 
865                 870                 875                 880 


Met Lys Gln Tyr Ser Asp Glu Lys Asn Pro Leu Ala Lys Tyr Tyr Glu 
                885                 890                 895     


Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn Gly Pro 
            900                 905                 910         


Ile Val Lys Lys Ile Lys Leu Leu Gly Asn Lys Val Gly Asn His Leu 
        915                 920                 925             


Asp Val Thr Asn Lys Tyr Glu Asn Ser Thr Lys Lys Leu Val Lys Leu 
    930                 935                 940                 


Ser Ile Lys Asn Tyr Arg Phe Asp Val Tyr Leu Thr Glu Lys Gly Tyr 
945                 950                 955                 960 


Lys Phe Val Thr Ile Ala Tyr Leu Asn Val Phe Lys Lys Asp Asn Tyr 
                965                 970                 975     


Tyr Tyr Ile Pro Lys Asp Lys Tyr Gln Glu Leu Lys Glu Lys Lys Lys 
            980                 985                 990         


Ile Lys Asp Thr Asp Gln Phe Ile  Ala Ser Phe Tyr Lys  Asn Asp Leu 
        995                 1000                 1005             


Ile Lys  Leu Asn Gly Asp Leu  Tyr Lys Ile Ile Gly  Val Asn Ser 
    1010                 1015                 1020             


Asp Asp  Arg Asn Ile Ile Glu  Leu Asp Tyr Tyr Asp  Ile Lys Tyr 
    1025                 1030                 1035             


Lys Asp  Tyr Cys Glu Ile Asn  Asn Ile Lys Gly Glu  Pro Arg Ile 
    1040                 1045                 1050             


Lys Lys  Thr Ile Gly Lys Lys  Thr Glu Ser Ile Glu  Lys Phe Thr 
    1055                 1060                 1065             


Thr Asp  Val Leu Gly Asn Leu  Tyr Leu His Ser Thr  Glu Lys Ala 
    1070                 1075                 1080             


Pro Gln  Leu Ile Phe Lys Arg  Gly Leu Gly Gly Ser  Gly Gly Ser 
    1085                 1090                 1095             


Gly Gly  Ser Gly Pro Pro Lys  Lys Lys Arg Lys Val  Gly Gly Gly 
    1100                 1105                 1110             


Gly Gly  Ser His His His His  His His 
    1115                 1120         


<210>  38
<211>  3410
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  GST3-v1, nucleotide

<400>  38
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcccctaaga       60

agaagcgcaa ggtcatgaac cagaagttca ttctcggtct ggacattggc attactagcg      120

tgggatacgg cttgattgac tacgagacta agaacatcat cgatgccggc gtccgcctgt      180

tcccggaagc caacgtggag aacaatgagg gccggaggtc gaagagaggc tcccgccgcc      240

tgaagcggcg gcgaatccac cggctggaga gagtgaagct gctgctcacc gaatacgacc      300

tgatcaacaa agaacagatc ccgacctcca acaacccgta ccagatcaga gtgaagggtc      360

tgtcagaaat cctgtccaag gacgaactgg caatcgccct gctgcacctg gccaagcggc      420

gcggaatcca caacgtggat gtggctgccg acaaggaaga aaccgcttcc gactccctga      480

gcactaagga ccagatcaac aagaacgcca agttcttgga gtcccgctac gtgtgcgagc      540

tgcagaagga acggctggaa aacgaaggtc acgtgcgcgg agtggaaaac cggttcctga      600

caaaggacat tgtgcgcgaa gcgaagaaga tcattgatac ccaaatgcag tactaccctg      660

aaatcgacga gactttcaag gaaaagtaca tttccctggt ggaaacccgg cgggaatact      720

tcgaaggccc cggacaggga tcgccgttcg gatggaacgg ggacctcaag aagtggtacg      780

agatgctgat ggggcactgt acctactttc cgcaagaact gcgctccgtg aagtacgcgt      840

actccgcgga tctcttcaac gcgttgaatg acctgaacaa cctgatcatt cagagagaca      900

attccgaaaa gctcgagtac cacgagaagt atcacatcat cgagaatgtg ttcaagcaga      960

agaagaaacc gaccctcaag caaatcgcca aggagattgg cgtcaaccca gaggacatca     1020

agggatatcg gattaccaag agcggcactc ccgagtttac ctctttcaag ctgtttcatg     1080

atctgaagaa agtcgtgaag gaccatgcca ttctcgacga cattgatctc ctgaatcaga     1140

tcgcagagat cctgactatc taccaagaca aggactcgat tgtggcagag ctgggtcagc     1200

tcgaatacct gatgtccgag gccgacaagc agtccatctc cgaactgaca gggtacacgg     1260

ggactcatag cctgtcgctg aagtgcatga acatgatcat tgatgaactg tggcacagct     1320

ccatgaacca aatggaagtg tttacctacc tcaacatgcg ccctaagaag tacgaactga     1380

aaggctacca gcgcatcccc accgacatga tcgacgacgc gatcttgtcc cctgtggtca     1440

agaggacctt cattcaatcc atcaacgtga tcaacaaggt catcgaaaag tacggaatac     1500

cagaagatat catcattgag ctcgctcggg agaacaactc ggatgaccgg aagaagttca     1560

tcaacaatct tcagaagaag aacgaagcga ctcggaaacg gatcaacgag atcatcggac     1620

agaccggaaa ccagaacgcc aaacggattg tcgaaaagat tagactgcac gaccagcagg     1680

aagggaagtg cctgtactca ctcgagtcaa taccgctcga ggacctgttg aacaacccta     1740

accactatga agtggaccac atcatccctc ggtccgtgag cttcgacaac tcgtaccaca     1800

acaaagtgct cgtgaagcag tccgaaaact ccaagaaatc caacctgacc ccgtaccaat     1860

acttcaattc gggaaagtcg aagctgtcgt acaaccagtt caaacaacac atactcaacc     1920

ttagcaaaag ccaggatcgc atttccaaga agaagaagga atacctcctc gaggaaaggg     1980

acatcaacaa gttcgaagtg cagaaagagt tcatcaatcg caacttggtg gataccagat     2040

atgccacccg ggaactcacc agctatctca aggcctactt ttccgccaac aacatggacg     2100

tgaaggtcaa gaccatcaac gggtccttca ctaaccatct gagaaaggtc tggcggtttg     2160

acaagtaccg caaccacgga tacaagcacc acgctgagga cgctctgatc atcgccaatg     2220

ccgacttcct gttcaaggaa aacaagaagc tgcagaacac gaacaagatt ctggaaaagc     2280

ctaccattga gaacaacact aagaaggtca ccgtggagaa ggaagaggac tacaacaacg     2340

tgttcgaaac tcctaaactg gtggaggata tcaagcaata ccgcgactac aagttctcac     2400

accgggtgga caagaaaccg aatagacagc tgatcaacga cacgttgtat tccacccgga     2460

tgaaggatga gcatgactac attgtgcaga ctatcaccga tatctacgga aaagataaca     2520

ctaacctgaa gaaacaattc aacaagaacc cagagaagtt cctgatgtac cagaacgacc     2580

ccaagacctt tgagaagctt tccatcatca tgaagcagta ctccgacgag aagaacccgc     2640

tggccaagta ctacgaagaa accggagaat acctgaccaa gtacagcaag aagaacaacg     2700

gtcccattgt caagaagatc aagctgctcg gcaacaaggt cggaaaccac ctcgacgtga     2760

caaacaagta cgagaactcg actaagaagc ttgtgaagct gtcaatcaag aactatagat     2820

tcgacgtgta cttgaccgaa aagggataca agttcgtgac catagcctat ctgaacgtgt     2880

tcaagaaaga taactactac tacatcccca aggacaagta ccaggagctc aaagaaaaga     2940

agaagatcaa agacaccgac cagttcattg cctccttcta caagaacgac ctgatcaaac     3000

tgaacggcga cctctacaag atcattggag tgaacagcga tgacaggaac atcattgagc     3060

tggactacta cgacatcaag tacaaggact actgcgagat caacaacatc aagggcgaac     3120

cccggatcaa gaaaactatt ggaaagaaaa ccgagtccat tgagaagttc accactgacg     3180

tgctgggaaa cctttacctc cactccaccg agaaggcacc acaactgatc ttcaagcgcg     3240

gcctgaaacg gcccgccgca accaagaagg ccggccaggc gaagaagaag aaatgagcgg     3300

ccgcttaatt aagctgcctt ctgcggggct tgccttctgg ccatgccctt cttctctccc     3360

ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaagtctag                3410


<210>  39
<211>  1082
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  GST3-v1, amino acid

<400>  39

Met Ala Pro Lys Lys Lys Arg Lys Val Met Asn Gln Lys Phe Ile Leu 
1               5                   10                  15      


Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr 
            20                  25                  30          


Glu Thr Lys Asn Ile Ile Asp Ala Gly Val Arg Leu Phe Pro Glu Ala 
        35                  40                  45              


Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg 
    50                  55                  60                  


Leu Lys Arg Arg Arg Ile His Arg Leu Glu Arg Val Lys Leu Leu Leu 
65                  70                  75                  80  


Thr Glu Tyr Asp Leu Ile Asn Lys Glu Gln Ile Pro Thr Ser Asn Asn 
                85                  90                  95      


Pro Tyr Gln Ile Arg Val Lys Gly Leu Ser Glu Ile Leu Ser Lys Asp 
            100                 105                 110         


Glu Leu Ala Ile Ala Leu Leu His Leu Ala Lys Arg Arg Gly Ile His 
        115                 120                 125             


Asn Val Asp Val Ala Ala Asp Lys Glu Glu Thr Ala Ser Asp Ser Leu 
    130                 135                 140                 


Ser Thr Lys Asp Gln Ile Asn Lys Asn Ala Lys Phe Leu Glu Ser Arg 
145                 150                 155                 160 


Tyr Val Cys Glu Leu Gln Lys Glu Arg Leu Glu Asn Glu Gly His Val 
                165                 170                 175     


Arg Gly Val Glu Asn Arg Phe Leu Thr Lys Asp Ile Val Arg Glu Ala 
            180                 185                 190         


Lys Lys Ile Ile Asp Thr Gln Met Gln Tyr Tyr Pro Glu Ile Asp Glu 
        195                 200                 205             


Thr Phe Lys Glu Lys Tyr Ile Ser Leu Val Glu Thr Arg Arg Glu Tyr 
    210                 215                 220                 


Phe Glu Gly Pro Gly Gln Gly Ser Pro Phe Gly Trp Asn Gly Asp Leu 
225                 230                 235                 240 


Lys Lys Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro Gln 
                245                 250                 255     


Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp Leu Phe Asn Ala 
            260                 265                 270         


Leu Asn Asp Leu Asn Asn Leu Ile Ile Gln Arg Asp Asn Ser Glu Lys 
        275                 280                 285             


Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn Val Phe Lys Gln 
    290                 295                 300                 


Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Gly Val Asn 
305                 310                 315                 320 


Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser Gly Thr Pro Glu 
                325                 330                 335     


Phe Thr Ser Phe Lys Leu Phe His Asp Leu Lys Lys Val Val Lys Asp 
            340                 345                 350         


His Ala Ile Leu Asp Asp Ile Asp Leu Leu Asn Gln Ile Ala Glu Ile 
        355                 360                 365             


Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Val Ala Glu Leu Gly Gln 
    370                 375                 380                 


Leu Glu Tyr Leu Met Ser Glu Ala Asp Lys Gln Ser Ile Ser Glu Leu 
385                 390                 395                 400 


Thr Gly Tyr Thr Gly Thr His Ser Leu Ser Leu Lys Cys Met Asn Met 
                405                 410                 415     


Ile Ile Asp Glu Leu Trp His Ser Ser Met Asn Gln Met Glu Val Phe 
            420                 425                 430         


Thr Tyr Leu Asn Met Arg Pro Lys Lys Tyr Glu Leu Lys Gly Tyr Gln 
        435                 440                 445             


Arg Ile Pro Thr Asp Met Ile Asp Asp Ala Ile Leu Ser Pro Val Val 
    450                 455                 460                 


Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn Lys Val Ile Glu 
465                 470                 475                 480 


Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn 
                485                 490                 495     


Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu Gln Lys Lys Asn 
            500                 505                 510         


Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly Gln Thr Gly Asn 
        515                 520                 525             


Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu His Asp Gln Gln 
    530                 535                 540                 


Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro Leu Glu Asp Leu 
545                 550                 555                 560 


Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile Ile Pro Arg Ser 
                565                 570                 575     


Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu Val Lys Gln Ser 
            580                 585                 590         


Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln Tyr Phe Asn Ser 
        595                 600                 605             


Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln His Ile Leu Asn 
    610                 615                 620                 


Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys Lys Glu Tyr Leu 
625                 630                 635                 640 


Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln Lys Glu Phe Ile 
                645                 650                 655     


Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu Thr Ser 
            660                 665                 670         


Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asp Val Lys Val Lys 
        675                 680                 685             


Thr Ile Asn Gly Ser Phe Thr Asn His Leu Arg Lys Val Trp Arg Phe 
    690                 695                 700                 


Asp Lys Tyr Arg Asn His Gly Tyr Lys His His Ala Glu Asp Ala Leu 
705                 710                 715                 720 


Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn Lys Lys Leu Gln 
                725                 730                 735     


Asn Thr Asn Lys Ile Leu Glu Lys Pro Thr Ile Glu Asn Asn Thr Lys 
            740                 745                 750         


Lys Val Thr Val Glu Lys Glu Glu Asp Tyr Asn Asn Val Phe Glu Thr 
        755                 760                 765             


Pro Lys Leu Val Glu Asp Ile Lys Gln Tyr Arg Asp Tyr Lys Phe Ser 
    770                 775                 780                 


His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu 
785                 790                 795                 800 


Tyr Ser Thr Arg Met Lys Asp Glu His Asp Tyr Ile Val Gln Thr Ile 
                805                 810                 815     


Thr Asp Ile Tyr Gly Lys Asp Asn Thr Asn Leu Lys Lys Gln Phe Asn 
            820                 825                 830         


Lys Asn Pro Glu Lys Phe Leu Met Tyr Gln Asn Asp Pro Lys Thr Phe 
        835                 840                 845             


Glu Lys Leu Ser Ile Ile Met Lys Gln Tyr Ser Asp Glu Lys Asn Pro 
    850                 855                 860                 


Leu Ala Lys Tyr Tyr Glu Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser 
865                 870                 875                 880 


Lys Lys Asn Asn Gly Pro Ile Val Lys Lys Ile Lys Leu Leu Gly Asn 
                885                 890                 895     


Lys Val Gly Asn His Leu Asp Val Thr Asn Lys Tyr Glu Asn Ser Thr 
            900                 905                 910         


Lys Lys Leu Val Lys Leu Ser Ile Lys Asn Tyr Arg Phe Asp Val Tyr 
        915                 920                 925             


Leu Thr Glu Lys Gly Tyr Lys Phe Val Thr Ile Ala Tyr Leu Asn Val 
    930                 935                 940                 


Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Lys Asp Lys Tyr Gln Glu 
945                 950                 955                 960 


Leu Lys Glu Lys Lys Lys Ile Lys Asp Thr Asp Gln Phe Ile Ala Ser 
                965                 970                 975     


Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asn Gly Asp Leu Tyr Lys Ile 
            980                 985                 990         


Ile Gly Val Asn Ser Asp Asp Arg  Asn Ile Ile Glu Leu  Asp Tyr Tyr 
        995                 1000                 1005             


Asp Ile  Lys Tyr Lys Asp Tyr  Cys Glu Ile Asn Asn  Ile Lys Gly 
    1010                 1015                 1020             


Glu Pro  Arg Ile Lys Lys Thr  Ile Gly Lys Lys Thr  Glu Ser Ile 
    1025                 1030                 1035             


Glu Lys  Phe Thr Thr Asp Val  Leu Gly Asn Leu Tyr  Leu His Ser 
    1040                 1045                 1050             


Thr Glu  Lys Ala Pro Gln Leu  Ile Phe Lys Arg Gly  Leu Lys Arg 
    1055                 1060                 1065             


Pro Ala  Ala Thr Lys Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1070                 1075                 1080         


<210>  40
<211>  3410
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  GST1-v1, nucleotide

<400>  40
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcccctaaga       60

agaagcgcaa ggtcatgaac cagaagttca ttctcggtct ggacattggc attactagcg      120

tgggatacgg cttgattgac tacgagacta agaacatcat cgatgccggc gtccgcctgt      180

tcccggaagc caacgtggag aacaatgagg gccggaggtc gaagagaggc tcccgccgcc      240

tgaagcggcg gcgaatccac cggctggaga gagtgaagct gctgctcacc gaatacgacc      300

tgatcaacaa agaacagatc ccgacctcca acaacccgta ccagatcaga gtgaagggtc      360

tgtcagaaat cctgtccaag gacgaactgg caatcgccct gctgcacctg gccaagcggc      420

gcggaatcca caacgtggat gtggctgccg acaaggaaga aaccgcttcc gactccctga      480

gcactaagga ccagatcaac aagaacgcca agttcttgga gtcccgctac gtgtgcgagc      540

tgcagaagga acggctggaa aacgaaggtc acgtgcgcgg agtggaaaac cggttcctga      600

caaaggacat tgtgcgcgaa gcgaagaaga tcattgatac ccaaatgcag tactaccctg      660

aaatcgacga gactttcaag gaaaagtaca tttccctggt ggaaacccgg cgggaatact      720

tcgaaggccc cggacaggga tcgccgttcg gatggaacgg ggacctcaag aagtggtacg      780

agatgctgat ggggcactgt acctactttc cgcaagaact gcgctccgtg aagtacgcgt      840

actccgcgga tctcttcaac gcgttgaatg acctgaacaa cctgatcatt cagagagaca      900

attccgaaaa gctcgagtac cacgagaagt atcacatcat cgagaatgtg ttcaagcaga      960

agaagaaacc gaccctcaag caaatcgcca aggagattgg cgtcaaccca gaggacatca     1020

agggatatcg gattaccaag agcggcactc ccgagtttac ctctttcaag ctgtttcatg     1080

atctgaagaa agtcgtgaag gaccatgcca ttctcgacga cattgatctc ctgaatcaga     1140

tcgcagagat cctgactatc taccaagaca aggactcgat tgtggcagag ctgggtcagc     1200

tcgaatacct gatgtccgag gccgacaagc agtccatctc cgaactgaca gggtacacgg     1260

ggactcatag cctgtcgctg aagtgcatga acatgatcat tgatgaactg tggcacagct     1320

ccatgaacca aatggaagtg tttacctacc tcaacatgcg ccctaagaag tacgaactga     1380

aaggctacca gcgcatcccc accgacatga tcgacgacgc gatcttgtcc cctgtggtca     1440

agaggacctt cattcaatcc atcaacgtga tcaacaaggt catcgaaaag tacggaatac     1500

cagaagatat catcattgag ctcgctcggg agaacaactc ggatgaccgg aagaagttca     1560

tcaacaatct tcagaagaag aacgaagcga ctcggaaacg gatcaacgag atcatcggac     1620

agaccggaaa ccagaacgcc aaacggattg tcgaaaagat tagactgcac gaccagcagg     1680

aagggaagtg cctgtactca ctcgagtcaa taccgctcga ggacctgttg aacaacccta     1740

accactatga agtggaccac atcatccctc ggtccgtgag cttcgacaac tcgtaccaca     1800

acaaagtgct cgtgaagcag tccgaaaact ccaagaaatc caacctgacc ccgtaccaat     1860

acttcaattc gggaaagtcg aagctgtcgt acaaccagtt caaacaacac atactcaacc     1920

ttagcaaaag ccaggatcgc atttccaaga agaagaagga atacctcctc gaggaaaggg     1980

acatcaacaa gttcgaagtg cagaaagagt tcatcaatcg caacttggtg gataccagat     2040

atgccacccg ggaactcacc aactatctca aggcctactt ttccgccaac aacatgaacg     2100

tgaaggtcaa gaccatcaac gggtccttca ctgactacct gagaaaggtc tggaagttca     2160

agaaggaacg caaccacgga tacaagcacc acgctgagga cgctctgatc atcgccaatg     2220

ccgacttcct gttcaaggaa aacaagaagc tgaaagctgt caactcagtg ctggaaaagc     2280

ctgaaatcga gactaagcag ctggatatcc aagtggactc tgaggacaac tacagcgaga     2340

tgttcatcat ccctaaacaa gtgcaggata tcaaggactt tcgcaacttc aagtactcac     2400

accgggtgga caagaaaccg aatagacagc tgatcaacga cacgttgtat tccacccgga     2460

agaaggataa ctcaacctac attgtgcaga ctatcaagga tatctacgcc aaagataaca     2520

ctactctgaa gaaacaattc gacaagtccc cagagaagtt cctgatgtac cagcacgacc     2580

cccgaacctt tgagaagctt gaagtgatca tgaagcagta cgccaacgag aagaacccgc     2640

tggccaagta ccatgaagaa accggagaat acctgaccaa gtacagcaag aagaacaacg     2700

gtcccattgt caagagcctg aagtacatcg gcaacaagct gggatcccac ctcgacgtga     2760

cacatcagtt caagtcgtcg actaagaagc ttgtgaagct gtcaatcaag aactatagat     2820

tcgacgtgta cttgaccgaa aagggataca agttcgtgac catagcctat ctgaacgtgt     2880

tcaagaaaga taactactac tacatcccca aggacaagta ccaggagctc aaagaaaaga     2940

agaagatcaa agacaccgac cagttcattg cctccttcta caagaacgac ctgatcaaac     3000

tgaacggcga cctctacaag atcattggag tgaacagcga tgacaggaac atcattgagc     3060

tggactacta cgacatcaag tacaaggact actgcgagat caacaacatc aagggcgaac     3120

cccggatcaa gaaaactatt ggaaagaaaa ccgagtccat tgagaagttc accactgacg     3180

tgctgggaaa cctttacctc cactccaccg agaaggcacc acaactgatc ttcaagcgcg     3240

gcctgaaacg gcccgccgca accaagaagg ccggccaggc gaagaagaag aaatgagcgg     3300

ccgcttaatt aagctgcctt ctgcggggct tgccttctgg ccatgccctt cttctctccc     3360

ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaagtctag                3410


<210>  41
<211>  1082
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  GST1-v1, amino acid

<400>  41

Met Ala Pro Lys Lys Lys Arg Lys Val Met Asn Gln Lys Phe Ile Leu 
1               5                   10                  15      


Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr 
            20                  25                  30          


Glu Thr Lys Asn Ile Ile Asp Ala Gly Val Arg Leu Phe Pro Glu Ala 
        35                  40                  45              


Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg 
    50                  55                  60                  


Leu Lys Arg Arg Arg Ile His Arg Leu Glu Arg Val Lys Leu Leu Leu 
65                  70                  75                  80  


Thr Glu Tyr Asp Leu Ile Asn Lys Glu Gln Ile Pro Thr Ser Asn Asn 
                85                  90                  95      


Pro Tyr Gln Ile Arg Val Lys Gly Leu Ser Glu Ile Leu Ser Lys Asp 
            100                 105                 110         


Glu Leu Ala Ile Ala Leu Leu His Leu Ala Lys Arg Arg Gly Ile His 
        115                 120                 125             


Asn Val Asp Val Ala Ala Asp Lys Glu Glu Thr Ala Ser Asp Ser Leu 
    130                 135                 140                 


Ser Thr Lys Asp Gln Ile Asn Lys Asn Ala Lys Phe Leu Glu Ser Arg 
145                 150                 155                 160 


Tyr Val Cys Glu Leu Gln Lys Glu Arg Leu Glu Asn Glu Gly His Val 
                165                 170                 175     


Arg Gly Val Glu Asn Arg Phe Leu Thr Lys Asp Ile Val Arg Glu Ala 
            180                 185                 190         


Lys Lys Ile Ile Asp Thr Gln Met Gln Tyr Tyr Pro Glu Ile Asp Glu 
        195                 200                 205             


Thr Phe Lys Glu Lys Tyr Ile Ser Leu Val Glu Thr Arg Arg Glu Tyr 
    210                 215                 220                 


Phe Glu Gly Pro Gly Gln Gly Ser Pro Phe Gly Trp Asn Gly Asp Leu 
225                 230                 235                 240 


Lys Lys Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro Gln 
                245                 250                 255     


Glu Leu Arg Ser Val Lys Tyr Ala Tyr Ser Ala Asp Leu Phe Asn Ala 
            260                 265                 270         


Leu Asn Asp Leu Asn Asn Leu Ile Ile Gln Arg Asp Asn Ser Glu Lys 
        275                 280                 285             


Leu Glu Tyr His Glu Lys Tyr His Ile Ile Glu Asn Val Phe Lys Gln 
    290                 295                 300                 


Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Gly Val Asn 
305                 310                 315                 320 


Pro Glu Asp Ile Lys Gly Tyr Arg Ile Thr Lys Ser Gly Thr Pro Glu 
                325                 330                 335     


Phe Thr Ser Phe Lys Leu Phe His Asp Leu Lys Lys Val Val Lys Asp 
            340                 345                 350         


His Ala Ile Leu Asp Asp Ile Asp Leu Leu Asn Gln Ile Ala Glu Ile 
        355                 360                 365             


Leu Thr Ile Tyr Gln Asp Lys Asp Ser Ile Val Ala Glu Leu Gly Gln 
    370                 375                 380                 


Leu Glu Tyr Leu Met Ser Glu Ala Asp Lys Gln Ser Ile Ser Glu Leu 
385                 390                 395                 400 


Thr Gly Tyr Thr Gly Thr His Ser Leu Ser Leu Lys Cys Met Asn Met 
                405                 410                 415     


Ile Ile Asp Glu Leu Trp His Ser Ser Met Asn Gln Met Glu Val Phe 
            420                 425                 430         


Thr Tyr Leu Asn Met Arg Pro Lys Lys Tyr Glu Leu Lys Gly Tyr Gln 
        435                 440                 445             


Arg Ile Pro Thr Asp Met Ile Asp Asp Ala Ile Leu Ser Pro Val Val 
    450                 455                 460                 


Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn Lys Val Ile Glu 
465                 470                 475                 480 


Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn 
                485                 490                 495     


Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu Gln Lys Lys Asn 
            500                 505                 510         


Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly Gln Thr Gly Asn 
        515                 520                 525             


Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu His Asp Gln Gln 
    530                 535                 540                 


Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ser Ile Pro Leu Glu Asp Leu 
545                 550                 555                 560 


Leu Asn Asn Pro Asn His Tyr Glu Val Asp His Ile Ile Pro Arg Ser 
                565                 570                 575     


Val Ser Phe Asp Asn Ser Tyr His Asn Lys Val Leu Val Lys Gln Ser 
            580                 585                 590         


Glu Asn Ser Lys Lys Ser Asn Leu Thr Pro Tyr Gln Tyr Phe Asn Ser 
        595                 600                 605             


Gly Lys Ser Lys Leu Ser Tyr Asn Gln Phe Lys Gln His Ile Leu Asn 
    610                 615                 620                 


Leu Ser Lys Ser Gln Asp Arg Ile Ser Lys Lys Lys Lys Glu Tyr Leu 
625                 630                 635                 640 


Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln Lys Glu Phe Ile 
                645                 650                 655     


Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu Thr Asn 
            660                 665                 670         


Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn Val Lys Val Lys 
        675                 680                 685             


Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys Val Trp Lys Phe 
    690                 695                 700                 


Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala Glu Asp Ala Leu 
705                 710                 715                 720 


Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn Lys Lys Leu Lys 
                725                 730                 735     


Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu Thr Lys Gln Leu 
            740                 745                 750         


Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu Met Phe Ile Ile 
        755                 760                 765             


Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn Phe Lys Tyr Ser 
    770                 775                 780                 


His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu 
785                 790                 795                 800 


Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile Val Gln Thr Ile 
                805                 810                 815     


Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys Lys Gln Phe Asp 
            820                 825                 830         


Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp Pro Arg Thr Phe 
        835                 840                 845             


Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn Glu Lys Asn Pro 
    850                 855                 860                 


Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser 
865                 870                 875                 880 


Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys Tyr Ile Gly Asn 
                885                 890                 895     


Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe Lys Ser Ser Thr 
            900                 905                 910         


Lys Lys Leu Val Lys Leu Ser Ile Lys Asn Tyr Arg Phe Asp Val Tyr 
        915                 920                 925             


Leu Thr Glu Lys Gly Tyr Lys Phe Val Thr Ile Ala Tyr Leu Asn Val 
    930                 935                 940                 


Phe Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Lys Asp Lys Tyr Gln Glu 
945                 950                 955                 960 


Leu Lys Glu Lys Lys Lys Ile Lys Asp Thr Asp Gln Phe Ile Ala Ser 
                965                 970                 975     


Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asn Gly Asp Leu Tyr Lys Ile 
            980                 985                 990         


Ile Gly Val Asn Ser Asp Asp Arg  Asn Ile Ile Glu Leu  Asp Tyr Tyr 
        995                 1000                 1005             


Asp Ile  Lys Tyr Lys Asp Tyr  Cys Glu Ile Asn Asn  Ile Lys Gly 
    1010                 1015                 1020             


Glu Pro  Arg Ile Lys Lys Thr  Ile Gly Lys Lys Thr  Glu Ser Ile 
    1025                 1030                 1035             


Glu Lys  Phe Thr Thr Asp Val  Leu Gly Asn Leu Tyr  Leu His Ser 
    1040                 1045                 1050             


Thr Glu  Lys Ala Pro Gln Leu  Ile Phe Lys Arg Gly  Leu Lys Arg 
    1055                 1060                 1065             


Pro Ala  Ala Thr Lys Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1070                 1075                 1080         


<210>  42
<211>  3396
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Slu, nucleotide

<400>  42
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcccccaaga       60

agaaacgcaa agtcatgaac caaaagttca ttctggggct cgatatcggc atcacctccg      120

tgggatatgg tctgatcgac tacgagacta agaacatcat cgacgctgga gtgcgactgt      180

tcccggaagc gaacgtggag aacaacgaag gccgcagatc caagcgcggg tccagaaggc      240

tcaagaggcg gaggatccat agactcgaaa gagtgaagaa gctccttgaa gattacaatc      300

tgttggacca gagccagatt ccccaaagca ccaacccgta cgccatcaga gtgaagggcc      360

tgtccgaagc cctgtcgaaa gatgaactgg tcattgccct gctgcatatt gccaaacggc      420

gcggaatcca taagatcgac gtgatagact ccaacgatga cgtgggcaac gaactgtcaa      480

ccaaggagca gctgaacaag aactcgaaac tgctgaagga caagttcgtc tgccaaattc      540

aactggaacg gatgaacgag ggacaagtca ggggagagaa aaaccggttc aagaccgcgg      600

acatcatcaa ggagatcatc caactcctga atgtgcagaa gaactttcac cagctggatg      660

aaaacttcat taacaagtac attgaactgg tggaaatgcg gagggagtac ttcgagggac      720

ctggaaaggg atccccttac ggctgggaag gggaccccaa ggcttggtac gaaacgctca      780

tgggccattg cacttacttt ccggacgaac tccggtccgt gaagtacgca tactctgccg      840

atctgttcaa tgcactcaac gaccttaaca acttggtgat ccagcgcgat ggcctgtcca      900

agttggaata ccacgaaaag tatcacatca tcgagaacgt gttcaagcag aaaaagaagc      960

caactctgaa gcagattgcc aacgaaatta acgtgaaccc cgaggatatc aagggatacc     1020

ggatcactaa gtccggcaaa ccacagttca ccgagttcaa gctgtaccac gatctgaagt     1080

cggtgctctt tgaccagtcc atcctggaaa acgaagatgt gctggaccag attgctgaga     1140

tcctgaccat ctaccaggac aaggactcga ttaagagcaa gctcacggag ctggacattc     1200

tgctgaacga agaggataag gagaacatcg cgcagctcac tggttacacc ggtacccacc     1260

gcttgtccct taagtgcatc cggctggtcc tcgaggaaca atggtactcc agccggaacc     1320

agatggagat cttcacgcac ttgaacatca agccgaagaa gattaacctg accgctgcga     1380

acaagatacc caaggccatg atcgacgagt ttatcctctc accggtggtc aagcgcacct     1440

tcggacaagc catcaacctc atcaacaaga ttatcgagaa gtacggcgtg cctgaggata     1500

tcatcatcga gctggctcgg gagaacaact caaaggataa gcagaagttc attaacgaga     1560

tgcagaaaaa gaacgagaac actcgcaagc ggattaatga gatcatcggt aaatacggga     1620

accagaacgc caagcggctt gtggaaaaga ttcggctcca cgacgagcag gagggaaagt     1680

gtctgtactc gctggagagc attcccctgg aggacctcct gaacaaccca aaccactacg     1740

aagtggatca cataatcccc cgcagcgtgt cattcgacaa ttcctaccat aacaaggtcc     1800

tcgtgaagca gtccgagaat agcaagaagt ccaacctgac tccgtaccag tacttcaact     1860

ccggcaaatc caagctgtcc tacaaccagt tcaaacagca catcctcaac ctgtcaaaga     1920

gccaggacag gatctcgaag aagaagaagg aataccttct cgaggaacgg gatatcaata     1980

agttcgaggt gcagaaggag tttatcaata gaaacctggt ggacactcgc tatgccaccc     2040

gcgaactgac caactacctg aaggcgtact tctccgccaa caacatgaac gtgaaggtca     2100

aaactattaa cggcagcttc accgactatc tgcgcaaggt ctggaagttc aagaaggaac     2160

gcaaccacgg ttacaagcac cacgcggaag atgcgctgat tatcgccaac gctgacttcc     2220

tgttcaagga aaacaagaag ctcaaggccg tgaactcagt gctcgagaag cctgaaatcg     2280

agactaagca gctggacatc caggtcgatt cggaagataa ctactccgaa atgttcatca     2340

tccctaagca agtgcaggac atcaaggact tcaggaattt caagtacagc catcgcgtgg     2400

acaagaagcc aaacagacag ctgatcaacg atacactgta ttccacccgg aagaaggaca     2460

actccaccta catcgtccaa accattaagg acatctacgc aaaggacaac accacgctta     2520

agaagcagtt cgacaagagc cccgaaaagt tcctcatgta ccagcacgac cccagaacct     2580

tcgagaagct tgaagtgatc atgaagcagt acgccaacga aaagaaccca ctggctaagt     2640

accacgagga aaccggcgaa tacctgacca agtactccaa aaagaacaac ggaccgatcg     2700

tcaagtccct gaagtacatt gggaacaagc tcggctcgca cctcgatgtg acccaccagt     2760

tcaagtcctc gaccaaaaag ctcgtgaagc tgtccatcaa gccgtaccgg ttcgacgtgt     2820

acctgactga caagggatat aagttcatca ccatttccta cctcgacgtg ttgaagaagg     2880

ataactacta ctacattccg gaacagaagt acgacaagct caagctcgga aaggccatcg     2940

acaaaaatgc gaagttcatc gcgagcttct acaagaatga cttgatcaag ctggatggcg     3000

aaatctacaa gatcatcggg gtcaactccg atacccgcaa catgattgag ctggatctgc     3060

ccgacattcg gtacaaggaa tactgcgagc tgaacaacat caagggagaa ccgcggatca     3120

agaaaaccat cggaaagaaa gtgaacagca tcgagaaact gactactgac gtcctgggaa     3180

acgtgttcac caacacacaa tacaccaaac cccagctgct gtttaagcgc gggaacaagc     3240

gccctgccgc aactaagaag gccggacagg ccaaaaagaa gaaatgagcg gccgcttaat     3300

taagctgcct tctgcggggc ttgccttctg gccatgccct tcttctctcc cttgcacctg     3360

tacctcttgg tctttgaata aagcctgagt aggaag                               3396


<210>  43
<211>  1079
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  Slu, amino acid

<400>  43

Met Ala Pro Lys Lys Lys Arg Lys Val Met Asn Gln Lys Phe Ile Leu 
1               5                   10                  15      


Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr 
            20                  25                  30          


Glu Thr Lys Asn Ile Ile Asp Ala Gly Val Arg Leu Phe Pro Glu Ala 
        35                  40                  45              


Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg 
    50                  55                  60                  


Leu Lys Arg Arg Arg Ile His Arg Leu Glu Arg Val Lys Lys Leu Leu 
65                  70                  75                  80  


Glu Asp Tyr Asn Leu Leu Asp Gln Ser Gln Ile Pro Gln Ser Thr Asn 
                85                  90                  95      


Pro Tyr Ala Ile Arg Val Lys Gly Leu Ser Glu Ala Leu Ser Lys Asp 
            100                 105                 110         


Glu Leu Val Ile Ala Leu Leu His Ile Ala Lys Arg Arg Gly Ile His 
        115                 120                 125             


Lys Ile Asp Val Ile Asp Ser Asn Asp Asp Val Gly Asn Glu Leu Ser 
    130                 135                 140                 


Thr Lys Glu Gln Leu Asn Lys Asn Ser Lys Leu Leu Lys Asp Lys Phe 
145                 150                 155                 160 


Val Cys Gln Ile Gln Leu Glu Arg Met Asn Glu Gly Gln Val Arg Gly 
                165                 170                 175     


Glu Lys Asn Arg Phe Lys Thr Ala Asp Ile Ile Lys Glu Ile Ile Gln 
            180                 185                 190         


Leu Leu Asn Val Gln Lys Asn Phe His Gln Leu Asp Glu Asn Phe Ile 
        195                 200                 205             


Asn Lys Tyr Ile Glu Leu Val Glu Met Arg Arg Glu Tyr Phe Glu Gly 
    210                 215                 220                 


Pro Gly Lys Gly Ser Pro Tyr Gly Trp Glu Gly Asp Pro Lys Ala Trp 
225                 230                 235                 240 


Tyr Glu Thr Leu Met Gly His Cys Thr Tyr Phe Pro Asp Glu Leu Arg 
                245                 250                 255     


Ser Val Lys Tyr Ala Tyr Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp 
            260                 265                 270         


Leu Asn Asn Leu Val Ile Gln Arg Asp Gly Leu Ser Lys Leu Glu Tyr 
        275                 280                 285             


His Glu Lys Tyr His Ile Ile Glu Asn Val Phe Lys Gln Lys Lys Lys 
    290                 295                 300                 


Pro Thr Leu Lys Gln Ile Ala Asn Glu Ile Asn Val Asn Pro Glu Asp 
305                 310                 315                 320 


Ile Lys Gly Tyr Arg Ile Thr Lys Ser Gly Lys Pro Gln Phe Thr Glu 
                325                 330                 335     


Phe Lys Leu Tyr His Asp Leu Lys Ser Val Leu Phe Asp Gln Ser Ile 
            340                 345                 350         


Leu Glu Asn Glu Asp Val Leu Asp Gln Ile Ala Glu Ile Leu Thr Ile 
        355                 360                 365             


Tyr Gln Asp Lys Asp Ser Ile Lys Ser Lys Leu Thr Glu Leu Asp Ile 
    370                 375                 380                 


Leu Leu Asn Glu Glu Asp Lys Glu Asn Ile Ala Gln Leu Thr Gly Tyr 
385                 390                 395                 400 


Thr Gly Thr His Arg Leu Ser Leu Lys Cys Ile Arg Leu Val Leu Glu 
                405                 410                 415     


Glu Gln Trp Tyr Ser Ser Arg Asn Gln Met Glu Ile Phe Thr His Leu 
            420                 425                 430         


Asn Ile Lys Pro Lys Lys Ile Asn Leu Thr Ala Ala Asn Lys Ile Pro 
        435                 440                 445             


Lys Ala Met Ile Asp Glu Phe Ile Leu Ser Pro Val Val Lys Arg Thr 
    450                 455                 460                 


Phe Gly Gln Ala Ile Asn Leu Ile Asn Lys Ile Ile Glu Lys Tyr Gly 
465                 470                 475                 480 


Val Pro Glu Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn Asn Ser Lys 
                485                 490                 495     


Asp Lys Gln Lys Phe Ile Asn Glu Met Gln Lys Lys Asn Glu Asn Thr 
            500                 505                 510         


Arg Lys Arg Ile Asn Glu Ile Ile Gly Lys Tyr Gly Asn Gln Asn Ala 
        515                 520                 525             


Lys Arg Leu Val Glu Lys Ile Arg Leu His Asp Glu Gln Glu Gly Lys 
    530                 535                 540                 


Cys Leu Tyr Ser Leu Glu Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn 
545                 550                 555                 560 


Pro Asn His Tyr Glu Val Asp His Ile Ile Pro Arg Ser Val Ser Phe 
                565                 570                 575     


Asp Asn Ser Tyr His Asn Lys Val Leu Val Lys Gln Ser Glu Asn Ser 
            580                 585                 590         


Lys Lys Ser Asn Leu Thr Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser 
        595                 600                 605             


Lys Leu Ser Tyr Asn Gln Phe Lys Gln His Ile Leu Asn Leu Ser Lys 
    610                 615                 620                 


Ser Gln Asp Arg Ile Ser Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu 
625                 630                 635                 640 


Arg Asp Ile Asn Lys Phe Glu Val Gln Lys Glu Phe Ile Asn Arg Asn 
                645                 650                 655     


Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu Thr Asn Tyr Leu Lys 
            660                 665                 670         


Ala Tyr Phe Ser Ala Asn Asn Met Asn Val Lys Val Lys Thr Ile Asn 
        675                 680                 685             


Gly Ser Phe Thr Asp Tyr Leu Arg Lys Val Trp Lys Phe Lys Lys Glu 
    690                 695                 700                 


Arg Asn His Gly Tyr Lys His His Ala Glu Asp Ala Leu Ile Ile Ala 
705                 710                 715                 720 


Asn Ala Asp Phe Leu Phe Lys Glu Asn Lys Lys Leu Lys Ala Val Asn 
                725                 730                 735     


Ser Val Leu Glu Lys Pro Glu Ile Glu Thr Lys Gln Leu Asp Ile Gln 
            740                 745                 750         


Val Asp Ser Glu Asp Asn Tyr Ser Glu Met Phe Ile Ile Pro Lys Gln 
        755                 760                 765             


Val Gln Asp Ile Lys Asp Phe Arg Asn Phe Lys Tyr Ser His Arg Val 
    770                 775                 780                 


Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr 
785                 790                 795                 800 


Arg Lys Lys Asp Asn Ser Thr Tyr Ile Val Gln Thr Ile Lys Asp Ile 
                805                 810                 815     


Tyr Ala Lys Asp Asn Thr Thr Leu Lys Lys Gln Phe Asp Lys Ser Pro 
            820                 825                 830         


Glu Lys Phe Leu Met Tyr Gln His Asp Pro Arg Thr Phe Glu Lys Leu 
        835                 840                 845             


Glu Val Ile Met Lys Gln Tyr Ala Asn Glu Lys Asn Pro Leu Ala Lys 
    850                 855                 860                 


Tyr His Glu Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn 
865                 870                 875                 880 


Asn Gly Pro Ile Val Lys Ser Leu Lys Tyr Ile Gly Asn Lys Leu Gly 
                885                 890                 895     


Ser His Leu Asp Val Thr His Gln Phe Lys Ser Ser Thr Lys Lys Leu 
            900                 905                 910         


Val Lys Leu Ser Ile Lys Pro Tyr Arg Phe Asp Val Tyr Leu Thr Asp 
        915                 920                 925             


Lys Gly Tyr Lys Phe Ile Thr Ile Ser Tyr Leu Asp Val Leu Lys Lys 
    930                 935                 940                 


Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln Lys Tyr Asp Lys Leu Lys Leu 
945                 950                 955                 960 


Gly Lys Ala Ile Asp Lys Asn Ala Lys Phe Ile Ala Ser Phe Tyr Lys 
                965                 970                 975     


Asn Asp Leu Ile Lys Leu Asp Gly Glu Ile Tyr Lys Ile Ile Gly Val 
            980                 985                 990         


Asn Ser Asp Thr Arg Asn Met Ile  Glu Leu Asp Leu Pro  Asp Ile Arg 
        995                 1000                 1005             


Tyr Lys  Glu Tyr Cys Glu Leu  Asn Asn Ile Lys Gly  Glu Pro Arg 
    1010                 1015                 1020             


Ile Lys  Lys Thr Ile Gly Lys  Lys Val Asn Ser Ile  Glu Lys Leu 
    1025                 1030                 1035             


Thr Thr  Asp Val Leu Gly Asn  Val Phe Thr Asn Thr  Gln Tyr Thr 
    1040                 1045                 1050             


Lys Pro  Gln Leu Leu Phe Lys  Arg Gly Asn Lys Arg  Pro Ala Ala 
    1055                 1060                 1065             


Thr Lys  Lys Ala Gly Gln Ala  Lys Lys Lys Lys 
    1070                 1075                 


<210>  44
<211>  3393
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  F8 nucleotide

<400>  44
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcccccaaga       60

agaaacgcaa agtcatgaac caaaagttca ttctggggct cgatatcggc atcacctccg      120

tgggatatgg tctgatcgac tacgagacta agaacatcat cgacgctgga gtgcgactgt      180

tcccggaagc gaacgtggag aacaacgaag gccgcagatc caagcgcggg tccagaaggc      240

tcaagaggcg gaggatccat agactcgaga gagtgaagtc gctcctttcg gaatacaaga      300

ttatcagcgg tcttgccccc accaacaacc aaccgtacaa catcagagtg aagggcctga      360

ccgaacagct gaccaaagat gaactggccg tcgccctgct gcatattgcc aaacggcgcg      420

gaatccataa gatcgacgtg attgacagca acgatgacgt gggaaacgag ctgtcaacca      480

aggaacagct taacaagaac agcaaattgc tgaaggacaa gtttgtctgc caaattcaac      540

tggaacggat gaacgaggga caagtcaggg gagagaaaaa ccggttcaag accgccgaca      600

tcatcaagga gatcatccaa ctgctgaacg tgcagaagaa cttccaccaa ctggatgaaa      660

acttcattaa caagtacatt gaactggtgg aaatgcggag ggagtacttc gagggacctg      720

gacagggatc ccctttcggc tggaatgggg accttaagaa gtggtacgaa atgttgatgg      780

gccattgcac ttactttccg caagaactcc ggtccgtgaa gtacgcatac tctgccgacc      840

tgttcaatgc actcaacgac cttaacaact tgatcatcca gcgcgataac tcggaaaagt      900

tggaatacca cgaaaagtat cacatcatcg agaacgtgtt caagcagaaa aagaagccaa      960

ctctgaagca gattgccaag gaaattggcg tgaatccgga ggatatcaag ggataccgga     1020

tcactaagtc cggcacgcca gagttcaccg agttcaagct gtaccacgat ctgaagtcgg     1080

tgctctttga ccagtccatc ctggaaaacg aagatgtgct ggaccagatt gctgagatcc     1140

tgaccatcta ccaggacaag gactcgatta agtccaagct caccgagctg gacattctgc     1200

tgaacgaaga agataaggag aacatcgcgc agctcaccgg ttacaatggt acccaccgct     1260

tgtcccttaa gtgcatccgc ctggtgctgg aggaacagtg gtactcgagc cggaaccaga     1320

tggagatctt cactcacttg aacatcaagc cgaaaaagat taacctgact gccgccaaca     1380

agatacccaa ggccatgatc gacgagttta tcctctcacc ggtggtcaag cgcaccttca     1440

ttcaatctat caacgtgatc aacaaggtca tcgagaagta cggcattcct gaggatatca     1500

tcatcgagct ggctcgggag aacaactcag acgataggaa gaagttcatt aacaacctcc     1560

agaaaaagaa cgaggccact cgcaagcgga ttaatgagat catcggtcag accgggaacc     1620

agaacgccaa gcggatcgtg gaaaagattc ggctccacga ccaacaggag ggaaagtgtc     1680

tgtactcgct ggagtcgatt gcactgatgg acctcctgaa caacccacag aactacgaag     1740

tcgatcacat aatcccccgc agcgtggcat tcgacaactc catccataac aaggtcctcg     1800

tgaagcagat cgagaatagc aagaagggga accggactcc gtaccagtac ctgaactcct     1860

ccgacgccaa gctgtcatac aatcagttca aacagcacat tctcaacctg tccaagtcaa     1920

aggacaggat ctccaagaag aagaaggact accttctcga ggaacgggat atcaataagt     1980

tcgaggtgca gaaggagttt atcaatagaa acctggtgga cactcgctat gccacccgcg     2040

aactgaccag ctacctgaag gcgtacttct ccgccaacaa catggacgtg aaggtcaaaa     2100

ctattaacgg cagcttcacc aaccatctgc gcaaggtctg gaggttcgac aagtaccgca     2160

accacggtta caagcaccac gcggaagatg cgctgattat cgccaacgct gacttcctgt     2220

tcaaggaaaa caagaagctc aaggccgtga actcagtgct cgagaagcct gaaatcgaga     2280

ctaagcagct ggacatccag gtcgattcgg aagataacta ctccgaaatg ttcatcatcc     2340

ctaagcaagt gcaggacatc aaggacttca ggaatttcaa gtacagccat cgcgtggaca     2400

agaagccaaa cagacagctg atcaacgata cactgtattc cacccggaag aaggacaact     2460

ccacctacat cgtccaaacc attaaggaca tctacgcaaa ggacaacacc acgcttaaga     2520

agcagttcga caagagcccc gaaaagttcc tcatgtacca gcacgacccc agaaccttcg     2580

agaagcttga agtgatcatg aagcagtacg ccaacgaaaa gaacccactg gctaagtacc     2640

acgaggaaac cggcgaatac ctgaccaagt actccaaaaa gaacaacgga ccgatcgtca     2700

agtccctgaa gtacattggg aacaagctcg gctcgcacct cgatgtgacc caccagttca     2760

agtcctcgac caaaaagctc gtgaagctgt ccatcaagcc gtaccggttc gacgtgtacc     2820

tgactgacaa gggatataag ttcatcacca tttcctacct cgacgtgttg aagaaggata     2880

actactacta cattccggaa cagaagtacg acaagctcaa gctcggaaag gccatcgaca     2940

aaaatgcgaa gttcatcgcg agcttctaca agaatgactt gatcaagctg gatggcgaaa     3000

tctacaagat catcggggtc aactccgata cccgcaacat gattgagctg gatctgcccg     3060

acattcggta caaggaatac tgcgagctga acaacatcaa gggagaaccg cggatcaaga     3120

aaaccatcgg aaagaaagtg aacagcatcg agaaactgac tactgacgtc ctgggaaacg     3180

tgttcaccaa cacacaatac accaaacccc agctgctgtt taagcgcggg aacaagcgcc     3240

ctgccgcaac taagaaggcc ggacaggcca aaaagaagaa atgagcggcc gcttaattaa     3300

gctgccttct gcggggcttg ccttctggcc atgcccttct tctctccctt gcacctgtac     3360

ctcttggtct ttgaataaag cctgagtagg aag                                  3393


<210>  45
<211>  1078
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  F8, amino acid

<400>  45

Met Ala Pro Lys Lys Lys Arg Lys Val Met Asn Gln Lys Phe Ile Leu 
1               5                   10                  15      


Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr 
            20                  25                  30          


Glu Thr Lys Asn Ile Ile Asp Ala Gly Val Arg Leu Phe Pro Glu Ala 
        35                  40                  45              


Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg 
    50                  55                  60                  


Leu Lys Arg Arg Arg Ile His Arg Leu Glu Arg Val Lys Ser Leu Leu 
65                  70                  75                  80  


Ser Glu Tyr Lys Ile Ile Ser Gly Leu Ala Pro Thr Asn Asn Gln Pro 
                85                  90                  95      


Tyr Asn Ile Arg Val Lys Gly Leu Thr Glu Gln Leu Thr Lys Asp Glu 
            100                 105                 110         


Leu Ala Val Ala Leu Leu His Ile Ala Lys Arg Arg Gly Ile His Lys 
        115                 120                 125             


Ile Asp Val Ile Asp Ser Asn Asp Asp Val Gly Asn Glu Leu Ser Thr 
    130                 135                 140                 


Lys Glu Gln Leu Asn Lys Asn Ser Lys Leu Leu Lys Asp Lys Phe Val 
145                 150                 155                 160 


Cys Gln Ile Gln Leu Glu Arg Met Asn Glu Gly Gln Val Arg Gly Glu 
                165                 170                 175     


Lys Asn Arg Phe Lys Thr Ala Asp Ile Ile Lys Glu Ile Ile Gln Leu 
            180                 185                 190         


Leu Asn Val Gln Lys Asn Phe His Gln Leu Asp Glu Asn Phe Ile Asn 
        195                 200                 205             


Lys Tyr Ile Glu Leu Val Glu Met Arg Arg Glu Tyr Phe Glu Gly Pro 
    210                 215                 220                 


Gly Gln Gly Ser Pro Phe Gly Trp Asn Gly Asp Leu Lys Lys Trp Tyr 
225                 230                 235                 240 


Glu Met Leu Met Gly His Cys Thr Tyr Phe Pro Gln Glu Leu Arg Ser 
                245                 250                 255     


Val Lys Tyr Ala Tyr Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp Leu 
            260                 265                 270         


Asn Asn Leu Ile Ile Gln Arg Asp Asn Ser Glu Lys Leu Glu Tyr His 
        275                 280                 285             


Glu Lys Tyr His Ile Ile Glu Asn Val Phe Lys Gln Lys Lys Lys Pro 
    290                 295                 300                 


Thr Leu Lys Gln Ile Ala Lys Glu Ile Gly Val Asn Pro Glu Asp Ile 
305                 310                 315                 320 


Lys Gly Tyr Arg Ile Thr Lys Ser Gly Thr Pro Glu Phe Thr Glu Phe 
                325                 330                 335     


Lys Leu Tyr His Asp Leu Lys Ser Val Leu Phe Asp Gln Ser Ile Leu 
            340                 345                 350         


Glu Asn Glu Asp Val Leu Asp Gln Ile Ala Glu Ile Leu Thr Ile Tyr 
        355                 360                 365             


Gln Asp Lys Asp Ser Ile Lys Ser Lys Leu Thr Glu Leu Asp Ile Leu 
    370                 375                 380                 


Leu Asn Glu Glu Asp Lys Glu Asn Ile Ala Gln Leu Thr Gly Tyr Asn 
385                 390                 395                 400 


Gly Thr His Arg Leu Ser Leu Lys Cys Ile Arg Leu Val Leu Glu Glu 
                405                 410                 415     


Gln Trp Tyr Ser Ser Arg Asn Gln Met Glu Ile Phe Thr His Leu Asn 
            420                 425                 430         


Ile Lys Pro Lys Lys Ile Asn Leu Thr Ala Ala Asn Lys Ile Pro Lys 
        435                 440                 445             


Ala Met Ile Asp Glu Phe Ile Leu Ser Pro Val Val Lys Arg Thr Phe 
    450                 455                 460                 


Ile Gln Ser Ile Asn Val Ile Asn Lys Val Ile Glu Lys Tyr Gly Ile 
465                 470                 475                 480 


Pro Glu Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp Asp 
                485                 490                 495     


Arg Lys Lys Phe Ile Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr Arg 
            500                 505                 510         


Lys Arg Ile Asn Glu Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala Lys 
        515                 520                 525             


Arg Ile Val Glu Lys Ile Arg Leu His Asp Gln Gln Glu Gly Lys Cys 
    530                 535                 540                 


Leu Tyr Ser Leu Glu Ser Ile Ala Leu Met Asp Leu Leu Asn Asn Pro 
545                 550                 555                 560 


Gln Asn Tyr Glu Val Asp His Ile Ile Pro Arg Ser Val Ala Phe Asp 
                565                 570                 575     


Asn Ser Ile His Asn Lys Val Leu Val Lys Gln Ile Glu Asn Ser Lys 
            580                 585                 590         


Lys Gly Asn Arg Thr Pro Tyr Gln Tyr Leu Asn Ser Ser Asp Ala Lys 
        595                 600                 605             


Leu Ser Tyr Asn Gln Phe Lys Gln His Ile Leu Asn Leu Ser Lys Ser 
    610                 615                 620                 


Lys Asp Arg Ile Ser Lys Lys Lys Lys Asp Tyr Leu Leu Glu Glu Arg 
625                 630                 635                 640 


Asp Ile Asn Lys Phe Glu Val Gln Lys Glu Phe Ile Asn Arg Asn Leu 
                645                 650                 655     


Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu Thr Ser Tyr Leu Lys Ala 
            660                 665                 670         


Tyr Phe Ser Ala Asn Asn Met Asp Val Lys Val Lys Thr Ile Asn Gly 
        675                 680                 685             


Ser Phe Thr Asn His Leu Arg Lys Val Trp Arg Phe Asp Lys Tyr Arg 
    690                 695                 700                 


Asn His Gly Tyr Lys His His Ala Glu Asp Ala Leu Ile Ile Ala Asn 
705                 710                 715                 720 


Ala Asp Phe Leu Phe Lys Glu Asn Lys Lys Leu Lys Ala Val Asn Ser 
                725                 730                 735     


Val Leu Glu Lys Pro Glu Ile Glu Thr Lys Gln Leu Asp Ile Gln Val 
            740                 745                 750         


Asp Ser Glu Asp Asn Tyr Ser Glu Met Phe Ile Ile Pro Lys Gln Val 
        755                 760                 765             


Gln Asp Ile Lys Asp Phe Arg Asn Phe Lys Tyr Ser His Arg Val Asp 
    770                 775                 780                 


Lys Lys Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr Arg 
785                 790                 795                 800 


Lys Lys Asp Asn Ser Thr Tyr Ile Val Gln Thr Ile Lys Asp Ile Tyr 
                805                 810                 815     


Ala Lys Asp Asn Thr Thr Leu Lys Lys Gln Phe Asp Lys Ser Pro Glu 
            820                 825                 830         


Lys Phe Leu Met Tyr Gln His Asp Pro Arg Thr Phe Glu Lys Leu Glu 
        835                 840                 845             


Val Ile Met Lys Gln Tyr Ala Asn Glu Lys Asn Pro Leu Ala Lys Tyr 
    850                 855                 860                 


His Glu Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn Asn 
865                 870                 875                 880 


Gly Pro Ile Val Lys Ser Leu Lys Tyr Ile Gly Asn Lys Leu Gly Ser 
                885                 890                 895     


His Leu Asp Val Thr His Gln Phe Lys Ser Ser Thr Lys Lys Leu Val 
            900                 905                 910         


Lys Leu Ser Ile Lys Pro Tyr Arg Phe Asp Val Tyr Leu Thr Asp Lys 
        915                 920                 925             


Gly Tyr Lys Phe Ile Thr Ile Ser Tyr Leu Asp Val Leu Lys Lys Asp 
    930                 935                 940                 


Asn Tyr Tyr Tyr Ile Pro Glu Gln Lys Tyr Asp Lys Leu Lys Leu Gly 
945                 950                 955                 960 


Lys Ala Ile Asp Lys Asn Ala Lys Phe Ile Ala Ser Phe Tyr Lys Asn 
                965                 970                 975     


Asp Leu Ile Lys Leu Asp Gly Glu Ile Tyr Lys Ile Ile Gly Val Asn 
            980                 985                 990         


Ser Asp Thr Arg Asn Met Ile Glu  Leu Asp Leu Pro Asp  Ile Arg Tyr 
        995                 1000                 1005             


Lys Glu  Tyr Cys Glu Leu Asn  Asn Ile Lys Gly Glu  Pro Arg Ile 
    1010                 1015                 1020             


Lys Lys  Thr Ile Gly Lys Lys  Val Asn Ser Ile Glu  Lys Leu Thr 
    1025                 1030                 1035             


Thr Asp  Val Leu Gly Asn Val  Phe Thr Asn Thr Gln  Tyr Thr Lys 
    1040                 1045                 1050             


Pro Gln  Leu Leu Phe Lys Arg  Gly Asn Lys Arg Pro  Ala Ala Thr 
    1055                 1060                 1065             


Lys Lys  Ala Gly Gln Ala Lys  Lys Lys Lys 
    1070                 1075             


<210>  46
<211>  3405
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  E2, nucleotide

<400>  46
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcccccaaga       60

agaaacgcaa agtcatgaac caaaagttca ttctggggct cgatatcggc atcacctccg      120

tgggatatgg tctgatcgac tacgagacta agaacatcat cgacgctgga gtgcgactgt      180

tcccggaagc gaacgtggag aacaacgaag gccgcagatc caagcgcggg tccagaaggc      240

tcaagaggcg gaggatccat agactcgaca gagtgaagca cctccttgcc gaatacgatc      300

tgttggacct taccaacatt cccaagagca ccaacccgta ccaaaccaga gtgaagggcc      360

tgaacgaaaa gctgtcgaaa gatgaactgg tcattgccct gctgcatatt gccaaacggc      420

gcggaatcca taacgtggac gtggccgctg acaaggaaga gactgcgtcc gactcgctgt      480

caaccaagga ccagatcaac aagaacgcca aattcctgga aagccgctac gtctgcgagc      540

ttcaaaaaga acggctggag aacgagggac acgtcagggg agtggagaac cggttcctga      600

ccaaggacat cgtgcgggaa gccaagaaga tcatcgacac ccaaatgcag tattatccgg      660

aaattgatga aacttttaag gagaagtaca tttccctggt ggaaactcgg agggagtact      720

tcgagggacc tggaaaggga tcccctttcg gctgggaagg gaacattaag aagtggtttg      780

aacagatgat gggccattgc acttactttc cggaagaact ccggtccgtg aagtactcat      840

actctgccga gctgttcaat gcactcaacg accttaacaa cttggtgatc acccgcgatg      900

aagatgccaa gttgaactac ggagaaaagt tccagatcat cgagaacgtg ttcaagcaga      960

aaaagacccc aaatctgaag cagattgcca tcgaaattgg cgtgcacgag actgagatca     1020

agggataccg ggtcaacaag tccggcacgc cagagttcac cgagttcaag ctgtaccacg     1080

atctgaagtc gatcgtgttt gacaagtcca tcctggaaaa cgaagccatt ctggaccaga     1140

ttgctgagat cctgaccatc taccaggacg agcaatcgat taaggaagaa ctgaacaagc     1200

tccccgagat tctgaacgaa caggataagg ccgagatcgc gaagctcatt ggttacaatg     1260

gtacccaccg cttgtccctt aagtgcatcc atctgatcaa tgaggaactg tggcagacca     1320

gccggaacca gatggagatc ttcaattact tgaacatcaa gccgaacaag gtggacctgt     1380

ccgaacagaa caagataccc aaggacatgg tcaacgactt tatcctctca ccggtggtca     1440

agcgcacctt cattcaatct atcaacgtga tcaacaaggt catcgagaag tacggcattc     1500

ctgaggatat catcatcgag ctggctcggg agaacaactc agacgatagg aagaagttca     1560

ttaacaacct ccagaaaaag aacgaggcca ctcgcaagcg gattaatgag atcatcggtc     1620

agaccgggaa ccagaacgcc aagcggatcg tggaaaagat tcggctccac gaccaacagg     1680

agggaaagtg tctgtactcg ctgaaggaca ttcccctgga ggacctcctg aggaacccaa     1740

acaactacga catcgatcac ataatccccc gcagcgtgtc attcgacgat tccatgcata     1800

acaaggtcct cgtgcggaga gagcagaatg ccaagaagaa caaccagact ccgtaccagt     1860

acctgacgtc cggctacgca gacatcaagt actcagtgtt caaacagcac gtgctcaacc     1920

tggccgagaa caaggacagg atgaccaaga agaagcgcga ataccttctc gaggaacggg     1980

atatcaataa gttcgaggtg cagaaggagt ttatcaatag aaacctggtg gacactcgct     2040

atgccacccg cgaactgacc aactacctga aggcgtactt ctccgccaac aacatgaacg     2100

tgaaggtcaa aactattaac ggcagcttca ccgactatct gcgcaaggtc tggaagttca     2160

agaaggaacg caaccacggt tacaagcacc acgcggaaga tgcgctgatt atcgccaacg     2220

ctgacttcct gttcaaggaa aacaagaagc tcaaggccgt gaactcagtg ctcgagaagc     2280

ctgaaatcga gactaagcag ctggacatcc aggtcgattc ggaagataac tactccgaaa     2340

tgttcatcat ccctaagcaa gtgcaggaca tcaaggactt caggaatttc aagtacagcc     2400

atcgcgtgga caagaagcca aacagacagc tgatcaacga tacactgtat tccacccgga     2460

agaaggacaa ctccacctac atcgtccaaa ccattaagga catctacgca aaggacaaca     2520

ccacgcttaa gaagcagttc gacaagagcc ccgaaaagtt cctcatgtac cagcacgacc     2580

ccagaacctt cgagaagctt gaagtgatca tgaagcagta cgccaacgaa aagaacccac     2640

tggctaagta ccacgaggaa accggcgaat acctgaccaa gtactccaaa aagaacaacg     2700

gaccgatcgt caagtccctg aagtacattg ggaacaagct cggctcgcac ctcgatgtga     2760

cccaccagtt caagtcctcg accaaaaagc tcgtgaagct gtccatcaag ccgtaccggt     2820

tcgacgtgta cctgactgac aagggatata agttcatcac catttcctac ctcgacgtgt     2880

tgaagaagga taactactac tacattccgg aacagaagta cgacaagctc aagctcggaa     2940

aggccatcga caaaaatgcg aagttcatcg cgagcttcta caagaatgac ttgatcaagc     3000

tggatggcga aatctacaag atcatcgggg tcaactccga tacccgcaac atgattgagc     3060

tggatctgcc cgacattcgg tacaaggaat actgcgagct gaacaacatc aagggagaac     3120

cgcggatcaa gaaaaccatc ggaaagaaag tgaacagcat cgagaaactg actactgacg     3180

tcctgggaaa cgtgttcacc aacacacaat acaccaaacc ccagctgctg tttaagcgcg     3240

ggaacaagcg ccctgccgca actaagaagg ccggacaggc caaaaagaag aaatgagcgg     3300

ccgcttaatt aagctgcctt ctgcggggct tgccttctgg ccatgccctt cttctctccc     3360

ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaag                     3405


<210>  47
<211>  1082
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  E2, amino acid

<400>  47

Met Ala Pro Lys Lys Lys Arg Lys Val Met Asn Gln Lys Phe Ile Leu 
1               5                   10                  15      


Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr 
            20                  25                  30          


Glu Thr Lys Asn Ile Ile Asp Ala Gly Val Arg Leu Phe Pro Glu Ala 
        35                  40                  45              


Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg 
    50                  55                  60                  


Leu Lys Arg Arg Arg Ile His Arg Leu Asp Arg Val Lys His Leu Leu 
65                  70                  75                  80  


Ala Glu Tyr Asp Leu Leu Asp Leu Thr Asn Ile Pro Lys Ser Thr Asn 
                85                  90                  95      


Pro Tyr Gln Thr Arg Val Lys Gly Leu Asn Glu Lys Leu Ser Lys Asp 
            100                 105                 110         


Glu Leu Val Ile Ala Leu Leu His Ile Ala Lys Arg Arg Gly Ile His 
        115                 120                 125             


Asn Val Asp Val Ala Ala Asp Lys Glu Glu Thr Ala Ser Asp Ser Leu 
    130                 135                 140                 


Ser Thr Lys Asp Gln Ile Asn Lys Asn Ala Lys Phe Leu Glu Ser Arg 
145                 150                 155                 160 


Tyr Val Cys Glu Leu Gln Lys Glu Arg Leu Glu Asn Glu Gly His Val 
                165                 170                 175     


Arg Gly Val Glu Asn Arg Phe Leu Thr Lys Asp Ile Val Arg Glu Ala 
            180                 185                 190         


Lys Lys Ile Ile Asp Thr Gln Met Gln Tyr Tyr Pro Glu Ile Asp Glu 
        195                 200                 205             


Thr Phe Lys Glu Lys Tyr Ile Ser Leu Val Glu Thr Arg Arg Glu Tyr 
    210                 215                 220                 


Phe Glu Gly Pro Gly Lys Gly Ser Pro Phe Gly Trp Glu Gly Asn Ile 
225                 230                 235                 240 


Lys Lys Trp Phe Glu Gln Met Met Gly His Cys Thr Tyr Phe Pro Glu 
                245                 250                 255     


Glu Leu Arg Ser Val Lys Tyr Ser Tyr Ser Ala Glu Leu Phe Asn Ala 
            260                 265                 270         


Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asp Ala Lys 
        275                 280                 285             


Leu Asn Tyr Gly Glu Lys Phe Gln Ile Ile Glu Asn Val Phe Lys Gln 
    290                 295                 300                 


Lys Lys Thr Pro Asn Leu Lys Gln Ile Ala Ile Glu Ile Gly Val His 
305                 310                 315                 320 


Glu Thr Glu Ile Lys Gly Tyr Arg Val Asn Lys Ser Gly Thr Pro Glu 
                325                 330                 335     


Phe Thr Glu Phe Lys Leu Tyr His Asp Leu Lys Ser Ile Val Phe Asp 
            340                 345                 350         


Lys Ser Ile Leu Glu Asn Glu Ala Ile Leu Asp Gln Ile Ala Glu Ile 
        355                 360                 365             


Leu Thr Ile Tyr Gln Asp Glu Gln Ser Ile Lys Glu Glu Leu Asn Lys 
    370                 375                 380                 


Leu Pro Glu Ile Leu Asn Glu Gln Asp Lys Ala Glu Ile Ala Lys Leu 
385                 390                 395                 400 


Ile Gly Tyr Asn Gly Thr His Arg Leu Ser Leu Lys Cys Ile His Leu 
                405                 410                 415     


Ile Asn Glu Glu Leu Trp Gln Thr Ser Arg Asn Gln Met Glu Ile Phe 
            420                 425                 430         


Asn Tyr Leu Asn Ile Lys Pro Asn Lys Val Asp Leu Ser Glu Gln Asn 
        435                 440                 445             


Lys Ile Pro Lys Asp Met Val Asn Asp Phe Ile Leu Ser Pro Val Val 
    450                 455                 460                 


Lys Arg Thr Phe Ile Gln Ser Ile Asn Val Ile Asn Lys Val Ile Glu 
465                 470                 475                 480 


Lys Tyr Gly Ile Pro Glu Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn 
                485                 490                 495     


Asn Ser Asp Asp Arg Lys Lys Phe Ile Asn Asn Leu Gln Lys Lys Asn 
            500                 505                 510         


Glu Ala Thr Arg Lys Arg Ile Asn Glu Ile Ile Gly Gln Thr Gly Asn 
        515                 520                 525             


Gln Asn Ala Lys Arg Ile Val Glu Lys Ile Arg Leu His Asp Gln Gln 
    530                 535                 540                 


Glu Gly Lys Cys Leu Tyr Ser Leu Lys Asp Ile Pro Leu Glu Asp Leu 
545                 550                 555                 560 


Leu Arg Asn Pro Asn Asn Tyr Asp Ile Asp His Ile Ile Pro Arg Ser 
                565                 570                 575     


Val Ser Phe Asp Asp Ser Met His Asn Lys Val Leu Val Arg Arg Glu 
            580                 585                 590         


Gln Asn Ala Lys Lys Asn Asn Gln Thr Pro Tyr Gln Tyr Leu Thr Ser 
        595                 600                 605             


Gly Tyr Ala Asp Ile Lys Tyr Ser Val Phe Lys Gln His Val Leu Asn 
    610                 615                 620                 


Leu Ala Glu Asn Lys Asp Arg Met Thr Lys Lys Lys Arg Glu Tyr Leu 
625                 630                 635                 640 


Leu Glu Glu Arg Asp Ile Asn Lys Phe Glu Val Gln Lys Glu Phe Ile 
                645                 650                 655     


Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu Thr Asn 
            660                 665                 670         


Tyr Leu Lys Ala Tyr Phe Ser Ala Asn Asn Met Asn Val Lys Val Lys 
        675                 680                 685             


Thr Ile Asn Gly Ser Phe Thr Asp Tyr Leu Arg Lys Val Trp Lys Phe 
    690                 695                 700                 


Lys Lys Glu Arg Asn His Gly Tyr Lys His His Ala Glu Asp Ala Leu 
705                 710                 715                 720 


Ile Ile Ala Asn Ala Asp Phe Leu Phe Lys Glu Asn Lys Lys Leu Lys 
                725                 730                 735     


Ala Val Asn Ser Val Leu Glu Lys Pro Glu Ile Glu Thr Lys Gln Leu 
            740                 745                 750         


Asp Ile Gln Val Asp Ser Glu Asp Asn Tyr Ser Glu Met Phe Ile Ile 
        755                 760                 765             


Pro Lys Gln Val Gln Asp Ile Lys Asp Phe Arg Asn Phe Lys Tyr Ser 
    770                 775                 780                 


His Arg Val Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu 
785                 790                 795                 800 


Tyr Ser Thr Arg Lys Lys Asp Asn Ser Thr Tyr Ile Val Gln Thr Ile 
                805                 810                 815     


Lys Asp Ile Tyr Ala Lys Asp Asn Thr Thr Leu Lys Lys Gln Phe Asp 
            820                 825                 830         


Lys Ser Pro Glu Lys Phe Leu Met Tyr Gln His Asp Pro Arg Thr Phe 
        835                 840                 845             


Glu Lys Leu Glu Val Ile Met Lys Gln Tyr Ala Asn Glu Lys Asn Pro 
    850                 855                 860                 


Leu Ala Lys Tyr His Glu Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser 
865                 870                 875                 880 


Lys Lys Asn Asn Gly Pro Ile Val Lys Ser Leu Lys Tyr Ile Gly Asn 
                885                 890                 895     


Lys Leu Gly Ser His Leu Asp Val Thr His Gln Phe Lys Ser Ser Thr 
            900                 905                 910         


Lys Lys Leu Val Lys Leu Ser Ile Lys Pro Tyr Arg Phe Asp Val Tyr 
        915                 920                 925             


Leu Thr Asp Lys Gly Tyr Lys Phe Ile Thr Ile Ser Tyr Leu Asp Val 
    930                 935                 940                 


Leu Lys Lys Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln Lys Tyr Asp Lys 
945                 950                 955                 960 


Leu Lys Leu Gly Lys Ala Ile Asp Lys Asn Ala Lys Phe Ile Ala Ser 
                965                 970                 975     


Phe Tyr Lys Asn Asp Leu Ile Lys Leu Asp Gly Glu Ile Tyr Lys Ile 
            980                 985                 990         


Ile Gly Val Asn Ser Asp Thr Arg  Asn Met Ile Glu Leu  Asp Leu Pro 
        995                 1000                 1005             


Asp Ile  Arg Tyr Lys Glu Tyr  Cys Glu Leu Asn Asn  Ile Lys Gly 
    1010                 1015                 1020             


Glu Pro  Arg Ile Lys Lys Thr  Ile Gly Lys Lys Val  Asn Ser Ile 
    1025                 1030                 1035             


Glu Lys  Leu Thr Thr Asp Val  Leu Gly Asn Val Phe  Thr Asn Thr 
    1040                 1045                 1050             


Gln Tyr  Thr Lys Pro Gln Leu  Leu Phe Lys Arg Gly  Asn Lys Arg 
    1055                 1060                 1065             


Pro Ala  Ala Thr Lys Lys Ala  Gly Gln Ala Lys Lys  Lys Lys 
    1070                 1075                 1080         


<210>  48
<211>  3396
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  P2H12, nucleotide

<400>  48
aggaaataag agagaaaaga agagtaagaa gaaatataag agccaccatg gcccccaaga       60

agaaacgcaa agtcatgaac caaaagttca ttctggggct cgatatcggc atcacctccg      120

tgggatatgg tctgatcgac tacgagacta agaacatcat cgacgctgga gtgcgactgt      180

tcccggaagc gaacgtggag aacaacgaag gccgcagatc caagcgcggg tccagaaggc      240

tcaagaggcg gaggatccat agactcgaga gagtgaagaa gctccttgaa gattacaatc      300

tgttggacca gtcacagatt ccccaaagca ccaacccgta cgccatcaga gtgaagggcc      360

tgtcagaagc actgtcgaaa gatgaactgg tcattgccct gctgcatatt gccaaacggc      420

gcggaatcca taacatcaac gtgtcgagcg aagatgagga cgcgtccaac gaactgtcaa      480

ccaaggaaca gatcaaccgg aacaacaaac tgctgaagga caaatacgtc tgcgaggtgc      540

agcttcaacg gctgaaagag ggacagatca ggggagagaa aaaccggttc aagaccaccg      600

acatccttaa ggagatcgac caactcctga aagtgcagaa ggactatcac aacctcgaca      660

ttgattttat caaccagtac aaggagattg tggaaactcg gagggagtac ttcgagggac      720

ctggaaaggg atccccttat ggctgggaag gggaccccaa ggcttggtac gaaaccctga      780

tgggccattg cacttacttt ccggatgaac tccggtccgt gaagtacgct tactctgccg      840

acctgttcaa tgcactcaac gaccttaaca acttggtgat ccaacgcgat ggtctttcca      900

agttggagta ccacgaaaag taccacatca tcgagaacgt gttcaagcag aaaaagaagc      960

caactctgaa gcagattgcc aacgaaatta acgtgaaccc cgaggatatc aagggatacc     1020

ggattaccaa gtccggcaaa ccagagttca cctcattcaa gctgtttcac gatctgaaga     1080

aggtcgtgaa ggaccacgcc atcctggatg acattgatct tctgaaccag attgctgaga     1140

tcctgaccat ctaccaggac aaggactcga ttgtggccga actgggacag ctcgagtacc     1200

tgatgtccga agccgataag cagtccatca gcgaactcac cggttacacc ggtacccact     1260

ccttgtccct taagtgcatg aacatgatca ttgacgaact gtggcactcc agcatgaacc     1320

agatggaggt gttcacctac ttgaacatgc gcccgaagaa gtacgagctg aagggctacc     1380

agcgcatacc cacggacatg atcgacgacg ccatcctctc accggtggtc aagcgcacct     1440

tcattcaatc tatcaacgtg atcaacaagg tcatcgagaa gtacggcatt cctgaggata     1500

tcatcatcga gctggctcgg gagaacaact cagacgatag gaagaagttc attaacaacc     1560

tccagaaaaa gaacgaggcc actcgcaagc ggattaatga gatcatcggt cagaccggga     1620

accagaacgc caagcggatc gtggaaaaga ttcggctcca cgaccaacag gagggaaagt     1680

gtctgtactc gctggagtcc attcccctgg aggacctcct gaacaaccca aaccactacg     1740

aggtcgatca cataatcccc cgcagcgtgt cattcgacaa ctcctaccat aacaaggtcc     1800

tcgtgaagca gtcggagaat agcaagaagt cgaacctgac tccgtaccag tacttcaact     1860

ccggcaaatc caagctgtcc tacaatcagt tcaaacagca cattctcaac ctgtccaaga     1920

gccaggacag gatttcgaag aagaagaagg aataccttct cgaggaacgg gatatcaata     1980

agttcgaggt gcagaaggag tttatcaata gaaacctggt ggacactcgc tatgccaccc     2040

gcgaactgac caactacctg aaggcgtact tctccgccaa caacatgaac gtgaaggtca     2100

aaactattaa cggcagcttc accgactatc tgcgcaaggt ctggaagttc aagaaggaac     2160

gcaaccacgg ttacaagcac cacgcggaag atgcgctgat tatcgccaac gctgacttcc     2220

tgttcaagga aaacaagaag ctcaaggccg tgaactcagt gctcgagaag cctgaaatcg     2280

agactaagca gctggacatc caggtcgatt cggaagataa ctactccgaa atgttcatca     2340

tccctaagca agtgcaggac atcaaggact tcaggaattt caagtacagc catcgcgtgg     2400

acaagaagcc aaacagacag ctgatcaacg atacactgta ttccacccgg aagaaggaca     2460

actccaccta catcgtccaa accattaagg acatctacgc aaaggacaac accacgctta     2520

agaagcagtt cgacaagagc cccgaaaagt tcctcatgta ccagcacgac cccagaacct     2580

tcgagaagct tgaagtgatc atgaagcagt acgccaacga aaagaaccca ctggctaagt     2640

accacgagga aaccggcgaa tacctgacca agtactccaa aaagaacaac ggaccgatcg     2700

tcaagtccct gaagtacatt gggaacaagc tcggctcgca cctcgatgtg acccaccagt     2760

tcaagtcctc gaccaaaaag ctcgtgaagc tgtccatcaa gccgtaccgg ttcgacgtgt     2820

acctgactga caagggatat aagttcatca ccatttccta cctcgacgtg ttgaagaagg     2880

ataactacta ctacattccg gaacagaagt acgacaagct caagctcgga aaggccatcg     2940

acaaaaatgc gaagttcatc gcgagcttct acaagaatga cttgatcaag ctggatggcg     3000

aaatctacaa gatcatcggg gtcaactccg atacccgcaa catgattgag ctggatctgc     3060

ccgacattcg gtacaaggaa tactgcgagc tgaacaacat caagggagaa ccgcggatca     3120

agaaaaccat cggaaagaaa gtgaacagca tcgagaaact gactactgac gtcctgggaa     3180

acgtgttcac caacacacaa tacaccaaac cccagctgct gtttaagcgc gggaacaagc     3240

gccctgccgc aactaagaag gccggacagg ccaaaaagaa gaaatgagcg gccgcttaat     3300

taagctgcct tctgcggggc ttgccttctg gccatgccct tcttctctcc cttgcacctg     3360

tacctcttgg tctttgaata aagcctgagt aggaag                               3396


<210>  49
<211>  1079
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  P2H12, amino acid

<400>  49

Met Ala Pro Lys Lys Lys Arg Lys Val Met Asn Gln Lys Phe Ile Leu 
1               5                   10                  15      


Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile Asp Tyr 
            20                  25                  30          


Glu Thr Lys Asn Ile Ile Asp Ala Gly Val Arg Leu Phe Pro Glu Ala 
        35                  40                  45              


Asn Val Glu Asn Asn Glu Gly Arg Arg Ser Lys Arg Gly Ser Arg Arg 
    50                  55                  60                  


Leu Lys Arg Arg Arg Ile His Arg Leu Glu Arg Val Lys Lys Leu Leu 
65                  70                  75                  80  


Glu Asp Tyr Asn Leu Leu Asp Gln Ser Gln Ile Pro Gln Ser Thr Asn 
                85                  90                  95      


Pro Tyr Ala Ile Arg Val Lys Gly Leu Ser Glu Ala Leu Ser Lys Asp 
            100                 105                 110         


Glu Leu Val Ile Ala Leu Leu His Ile Ala Lys Arg Arg Gly Ile His 
        115                 120                 125             


Asn Ile Asn Val Ser Ser Glu Asp Glu Asp Ala Ser Asn Glu Leu Ser 
    130                 135                 140                 


Thr Lys Glu Gln Ile Asn Arg Asn Asn Lys Leu Leu Lys Asp Lys Tyr 
145                 150                 155                 160 


Val Cys Glu Val Gln Leu Gln Arg Leu Lys Glu Gly Gln Ile Arg Gly 
                165                 170                 175     


Glu Lys Asn Arg Phe Lys Thr Thr Asp Ile Leu Lys Glu Ile Asp Gln 
            180                 185                 190         


Leu Leu Lys Val Gln Lys Asp Tyr His Asn Leu Asp Ile Asp Phe Ile 
        195                 200                 205             


Asn Gln Tyr Lys Glu Ile Val Glu Thr Arg Arg Glu Tyr Phe Glu Gly 
    210                 215                 220                 


Pro Gly Lys Gly Ser Pro Tyr Gly Trp Glu Gly Asp Pro Lys Ala Trp 
225                 230                 235                 240 


Tyr Glu Thr Leu Met Gly His Cys Thr Tyr Phe Pro Asp Glu Leu Arg 
                245                 250                 255     


Ser Val Lys Tyr Ala Tyr Ser Ala Asp Leu Phe Asn Ala Leu Asn Asp 
            260                 265                 270         


Leu Asn Asn Leu Val Ile Gln Arg Asp Gly Leu Ser Lys Leu Glu Tyr 
        275                 280                 285             


His Glu Lys Tyr His Ile Ile Glu Asn Val Phe Lys Gln Lys Lys Lys 
    290                 295                 300                 


Pro Thr Leu Lys Gln Ile Ala Asn Glu Ile Asn Val Asn Pro Glu Asp 
305                 310                 315                 320 


Ile Lys Gly Tyr Arg Ile Thr Lys Ser Gly Lys Pro Glu Phe Thr Ser 
                325                 330                 335     


Phe Lys Leu Phe His Asp Leu Lys Lys Val Val Lys Asp His Ala Ile 
            340                 345                 350         


Leu Asp Asp Ile Asp Leu Leu Asn Gln Ile Ala Glu Ile Leu Thr Ile 
        355                 360                 365             


Tyr Gln Asp Lys Asp Ser Ile Val Ala Glu Leu Gly Gln Leu Glu Tyr 
    370                 375                 380                 


Leu Met Ser Glu Ala Asp Lys Gln Ser Ile Ser Glu Leu Thr Gly Tyr 
385                 390                 395                 400 


Thr Gly Thr His Ser Leu Ser Leu Lys Cys Met Asn Met Ile Ile Asp 
                405                 410                 415     


Glu Leu Trp His Ser Ser Met Asn Gln Met Glu Val Phe Thr Tyr Leu 
            420                 425                 430         


Asn Met Arg Pro Lys Lys Tyr Glu Leu Lys Gly Tyr Gln Arg Ile Pro 
        435                 440                 445             


Thr Asp Met Ile Asp Asp Ala Ile Leu Ser Pro Val Val Lys Arg Thr 
    450                 455                 460                 


Phe Ile Gln Ser Ile Asn Val Ile Asn Lys Val Ile Glu Lys Tyr Gly 
465                 470                 475                 480 


Ile Pro Glu Asp Ile Ile Ile Glu Leu Ala Arg Glu Asn Asn Ser Asp 
                485                 490                 495     


Asp Arg Lys Lys Phe Ile Asn Asn Leu Gln Lys Lys Asn Glu Ala Thr 
            500                 505                 510         


Arg Lys Arg Ile Asn Glu Ile Ile Gly Gln Thr Gly Asn Gln Asn Ala 
        515                 520                 525             


Lys Arg Ile Val Glu Lys Ile Arg Leu His Asp Gln Gln Glu Gly Lys 
    530                 535                 540                 


Cys Leu Tyr Ser Leu Glu Ser Ile Pro Leu Glu Asp Leu Leu Asn Asn 
545                 550                 555                 560 


Pro Asn His Tyr Glu Val Asp His Ile Ile Pro Arg Ser Val Ser Phe 
                565                 570                 575     


Asp Asn Ser Tyr His Asn Lys Val Leu Val Lys Gln Ser Glu Asn Ser 
            580                 585                 590         


Lys Lys Ser Asn Leu Thr Pro Tyr Gln Tyr Phe Asn Ser Gly Lys Ser 
        595                 600                 605             


Lys Leu Ser Tyr Asn Gln Phe Lys Gln His Ile Leu Asn Leu Ser Lys 
    610                 615                 620                 


Ser Gln Asp Arg Ile Ser Lys Lys Lys Lys Glu Tyr Leu Leu Glu Glu 
625                 630                 635                 640 


Arg Asp Ile Asn Lys Phe Glu Val Gln Lys Glu Phe Ile Asn Arg Asn 
                645                 650                 655     


Leu Val Asp Thr Arg Tyr Ala Thr Arg Glu Leu Thr Asn Tyr Leu Lys 
            660                 665                 670         


Ala Tyr Phe Ser Ala Asn Asn Met Asn Val Lys Val Lys Thr Ile Asn 
        675                 680                 685             


Gly Ser Phe Thr Asp Tyr Leu Arg Lys Val Trp Lys Phe Lys Lys Glu 
    690                 695                 700                 


Arg Asn His Gly Tyr Lys His His Ala Glu Asp Ala Leu Ile Ile Ala 
705                 710                 715                 720 


Asn Ala Asp Phe Leu Phe Lys Glu Asn Lys Lys Leu Lys Ala Val Asn 
                725                 730                 735     


Ser Val Leu Glu Lys Pro Glu Ile Glu Thr Lys Gln Leu Asp Ile Gln 
            740                 745                 750         


Val Asp Ser Glu Asp Asn Tyr Ser Glu Met Phe Ile Ile Pro Lys Gln 
        755                 760                 765             


Val Gln Asp Ile Lys Asp Phe Arg Asn Phe Lys Tyr Ser His Arg Val 
    770                 775                 780                 


Asp Lys Lys Pro Asn Arg Gln Leu Ile Asn Asp Thr Leu Tyr Ser Thr 
785                 790                 795                 800 


Arg Lys Lys Asp Asn Ser Thr Tyr Ile Val Gln Thr Ile Lys Asp Ile 
                805                 810                 815     


Tyr Ala Lys Asp Asn Thr Thr Leu Lys Lys Gln Phe Asp Lys Ser Pro 
            820                 825                 830         


Glu Lys Phe Leu Met Tyr Gln His Asp Pro Arg Thr Phe Glu Lys Leu 
        835                 840                 845             


Glu Val Ile Met Lys Gln Tyr Ala Asn Glu Lys Asn Pro Leu Ala Lys 
    850                 855                 860                 


Tyr His Glu Glu Thr Gly Glu Tyr Leu Thr Lys Tyr Ser Lys Lys Asn 
865                 870                 875                 880 


Asn Gly Pro Ile Val Lys Ser Leu Lys Tyr Ile Gly Asn Lys Leu Gly 
                885                 890                 895     


Ser His Leu Asp Val Thr His Gln Phe Lys Ser Ser Thr Lys Lys Leu 
            900                 905                 910         


Val Lys Leu Ser Ile Lys Pro Tyr Arg Phe Asp Val Tyr Leu Thr Asp 
        915                 920                 925             


Lys Gly Tyr Lys Phe Ile Thr Ile Ser Tyr Leu Asp Val Leu Lys Lys 
    930                 935                 940                 


Asp Asn Tyr Tyr Tyr Ile Pro Glu Gln Lys Tyr Asp Lys Leu Lys Leu 
945                 950                 955                 960 


Gly Lys Ala Ile Asp Lys Asn Ala Lys Phe Ile Ala Ser Phe Tyr Lys 
                965                 970                 975     


Asn Asp Leu Ile Lys Leu Asp Gly Glu Ile Tyr Lys Ile Ile Gly Val 
            980                 985                 990         


Asn Ser Asp Thr Arg Asn Met Ile  Glu Leu Asp Leu Pro  Asp Ile Arg 
        995                 1000                 1005             


Tyr Lys  Glu Tyr Cys Glu Leu  Asn Asn Ile Lys Gly  Glu Pro Arg 
    1010                 1015                 1020             


Ile Lys  Lys Thr Ile Gly Lys  Lys Val Asn Ser Ile  Glu Lys Leu 
    1025                 1030                 1035             


Thr Thr  Asp Val Leu Gly Asn  Val Phe Thr Asn Thr  Gln Tyr Thr 
    1040                 1045                 1050             


Lys Pro  Gln Leu Leu Phe Lys  Arg Gly Asn Lys Arg  Pro Ala Ala 
    1055                 1060                 1065             


Thr Lys  Lys Ala Gly Gln Ala  Lys Lys Lys Lys 
    1070                 1075                 


<210>  50
<211>  100
<212>  RNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  gRNA targeting albumin gene

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (98)..(99)
<223>  phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  phosphorothioate linkage

<400>  50
ugccaguucc cgaucguuac guuuuaguac ucuggaaaca gaaucuacug aaacaagaca       60

auaugucgug uuuaucccau caauuuauug gugggauuuu                            100


