                         SEQUENCE LISTING

<110>  Evonik Operations GmbH
 
<120>  Method for the fermentative production of L-lysine

<130>  file number: 2018E00276

<160>  19     

<170>  PatentIn version 3.5

<210>  1
<211>  2351
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  CDS
<222>  (1001)..(1348)
<223>  coding sequence of NCgl0275 (whiB4)

<220>
<221>  misc_feature
<222>  (1349)..(1351)
<223>  taa stop codon

<400>  1
agccctgcaa atccacacca tcatgctcat agaacctgcg atcctcaatc gaaacgatcg       60

catccttcat cgacgtagaa atctgatcac ccccaacctc aaaccgccgc tgcgcataaa      120

tataagcaat cggctggtca gtggaatcag taatcgtcgt gacgcccggc ccgcgaccat      180

ccgtcagatc tgaaaggttg gtctgcatcg tgtcattggt acgcgcaacc gcaacgccag      240

aaagactagc gaaaggcaca agtgcgagcg caccaaggac gccagcggcg actgtagatg      300

caacgagctt tgtcagagaa ttcgtggtgg acactccaac agcctagcga taaccccggg      360

aatcgtgaac ccaagactcc aacaatgtca gccaatcttt atttattcga ctgtgcgtta      420

ctgctgacag tcggcatgct ttgggaatcc accaggcaag ctatagggga ctgtagagct      480

gacgttcgga aaacgtgcag aaaaccacga actgcagtcc ggaaagcgcc ccactggccc      540

ttcgccggca gccacagagg ggttagacca tttattcgca actgtgctta cacccaagaa      600

ttctccaaca agaaaaatcc ctctatcaac attttaagtg aatgacctta agggtttgcg      660

gaatgcgatc catgttccaa ctcgcctcaa gcggtatgtg tgaagcaggt ttgaagccag      720

tgatgacctc tgatggggtt agtttgtggc aatttcgggg ctggctgaaa tgcctaattt      780

tccaaaactg aatttaatga gttgagctgt ggtaagacta gtgaactgca gatatgttgg      840

acaattgtaa agtcctctcg ttggaggctt ggcagtgtgg ccataaaagt attgcccccg      900

ttggtgtgat gcattcgaca gcaaattggc tgtgtgacta cacttgcgag tgtattaagt      960

attaggccgt gcatatgtag cgcattttaa ggagattgtc atg acg tct gtg att       1015
                                            Met Thr Ser Val Ile           
                                            1               5             

cca gag cag cgc aac aac ccc ttt tat agg gac agc gcc aca att gct       1063
Pro Glu Gln Arg Asn Asn Pro Phe Tyr Arg Asp Ser Ala Thr Ile Ala           
                10                  15                  20                

tcc tcg gac cac aca gag cgt ggt gag tgg gtc act cag gca aag tgt       1111
Ser Ser Asp His Thr Glu Arg Gly Glu Trp Val Thr Gln Ala Lys Cys           
            25                  30                  35                    

cga aat ggc gac cca gat gca ttg ttt gtt cgt ggt gca gcg caa cgc       1159
Arg Asn Gly Asp Pro Asp Ala Leu Phe Val Arg Gly Ala Ala Gln Arg           
        40                  45                  50                        

cga gca gca gca att tgc cgc cac tgc cct gta gcc atg cag tgc tgc       1207
Arg Ala Ala Ala Ile Cys Arg His Cys Pro Val Ala Met Gln Cys Cys           
    55                  60                  65                            

gcc gat gcc tta gat aac aag gtg gaa ttc gga gtc tgg gga ggc ctg       1255
Ala Asp Ala Leu Asp Asn Lys Val Glu Phe Gly Val Trp Gly Gly Leu           
70                  75                  80                  85            

acc gag cgc cag cgc cgt gca ttg ctt cga aag aag ccg cac att act       1303
Thr Glu Arg Gln Arg Arg Ala Leu Leu Arg Lys Lys Pro His Ile Thr           
                90                  95                  100               

aac tgg gct gaa tat ttg gct cag ggg ggc gag atc gcc ggg gtt           1348
Asn Trp Ala Glu Tyr Leu Ala Gln Gly Gly Glu Ile Ala Gly Val               
            105                 110                 115                   

taattaattt caagggctgg ccattaacgt ggtcggcttt tttgattcag ggcgcacccc     1408

aggcgcaccc caggcgcacc ccagcgcacc acaggcgcac cccagcgcac cacaggcgca     1468

ccagcccagc acaagagcca acgcaagtgt caggcacgcc agcaaagggg ctagcaagaa     1528

gcacgccccc gaagccttag aaatgcgttc cccggtagga tgattgccat gactaaatgg     1588

gaatacgcaa ctgtgccttt gattacgcat gcaactaagc agatcctcga tacttggggt     1648

gaggacggct gggagttggt ctcggtcatg cctggtatga accctgagaa cctcgttgct     1708

tacatgaagc gtgaggtggc ttagttctta tggcttctaa ttccgaacgc cttgcagagc     1768

tgggcatttc tcttccttcc gttgcagcgc ctgttgctgc gtatgttcct gcgattcaga     1828

ccggtaacca ggtgtggact tctggtcagc tgcctttcgt tgatggtcag cttccggcca     1888

ccggcaaggt tggcgctgag gtttccgctg aggatgcgga gaagttggct cgtgcggctg     1948

cgctaaacgc tcttgctgcg attgatgcgc ttgttggcat tgataaggtc actcgcgttt     2008

tgaagattgt tggtttcgtg gcgtctgctg atgatttcag tggtcagcct gctgtcgtca     2068

acggtgcttc caatttgatg ggtgaggttt tcggcgaggc tggggcgcat gcgcgttctg     2128

ctgtgggcgt ggcggagttg ccgctcaact cgcctgtcga ggtcgaggtt atcgtcgaga     2188

tcgcgcagta gcacgctttt cgacgcaaaa tgccggttcc gcacttcgtg cgaaaccggc     2248

atttttaaat tgaccctatc gtccccttag tgttcgtaag tagaaagatc gtggtctttt     2308

gattctctgg tgattgcgat ggcgatgatg gacactaggg aca                       2351


<210>  2
<211>  116
<212>  PRT
<213>  Corynebacterium glutamicum ATCC13032

<400>  2

Met Thr Ser Val Ile Pro Glu Gln Arg Asn Asn Pro Phe Tyr Arg Asp 
1               5                   10                  15      


Ser Ala Thr Ile Ala Ser Ser Asp His Thr Glu Arg Gly Glu Trp Val 
            20                  25                  30          


Thr Gln Ala Lys Cys Arg Asn Gly Asp Pro Asp Ala Leu Phe Val Arg 
        35                  40                  45              


Gly Ala Ala Gln Arg Arg Ala Ala Ala Ile Cys Arg His Cys Pro Val 
    50                  55                  60                  


Ala Met Gln Cys Cys Ala Asp Ala Leu Asp Asn Lys Val Glu Phe Gly 
65                  70                  75                  80  


Val Trp Gly Gly Leu Thr Glu Arg Gln Arg Arg Ala Leu Leu Arg Lys 
                85                  90                  95      


Lys Pro His Ile Thr Asn Trp Ala Glu Tyr Leu Ala Gln Gly Gly Glu 
            100                 105                 110         


Ile Ala Gly Val 
        115     


<210>  3
<211>  1266
<212>  DNA
<213>  Corynebacterium glutamicum ATCC13032


<220>
<221>  CDS
<222>  (1)..(1263)
<223>  cds of aspartokinase gene

<220>
<221>  misc_feature
<222>  (1264)..(1266)
<223>  taa stop codon

<400>  3
gtg gcc ctg gtc gta cag aaa tat ggc ggt tcc tcg ctt gag agt gcg         48
Met Ala Leu Val Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Ser Ala           
1               5                   10                  15                

gaa cgc att aga aac gtc gct gaa cgg atc gtt gcc acc aag aag gct         96
Glu Arg Ile Arg Asn Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala           
            20                  25                  30                    

gga aat gat gtc gtg gtt gtc tgc tcc gca atg gga gac acc acg gat        144
Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp           
        35                  40                  45                        

gaa ctt cta gaa ctt gca gcg gca gtg aat ccc gtt ccg cca gct cgt        192
Glu Leu Leu Glu Leu Ala Ala Ala Val Asn Pro Val Pro Pro Ala Arg           
    50                  55                  60                            

gaa atg gat atg ctc ctg act gct ggt gag cgt att tct aac gct ctc        240
Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu           
65                  70                  75                  80            

gtc gcc atg gct att gag tcc ctt ggc gca gaa gcc caa tct ttc acg        288
Val Ala Met Ala Ile Glu Ser Leu Gly Ala Glu Ala Gln Ser Phe Thr           
                85                  90                  95                

ggc tct cag gct ggt gtg ctc acc acc gag cgc cac gga aac gca cgc        336
Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg           
            100                 105                 110                   

att gtt gat gtc act cca ggt cgt gtg cgt gaa gca ctc gat gag ggc        384
Ile Val Asp Val Thr Pro Gly Arg Val Arg Glu Ala Leu Asp Glu Gly           
        115                 120                 125                       

aag atc tgc att gtt gct ggt ttc cag ggt gtt aat aaa gaa acc cgc        432
Lys Ile Cys Ile Val Ala Gly Phe Gln Gly Val Asn Lys Glu Thr Arg           
    130                 135                 140                           

gat gtc acc acg ttg ggt cgt ggt ggt tct gac acc act gca gtt gcg        480
Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala           
145                 150                 155                 160           

ttg gca gct gct ttg aac gct gat gtg tgt gag att tac tcg gac gtt        528
Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val           
                165                 170                 175               

gac ggt gtg tat acc gct gac ccg cgc atc gtt cct aat gca cag aag        576
Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys           
            180                 185                 190                   

ctg gaa aag ctc agc ttc gaa gaa atg ctg gaa ctt gct gct gtt ggc        624
Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly           
        195                 200                 205                       

tcc aag att ttg gtg ctg cgc agt gtt gaa tac gct cgt gca ttc aat        672
Ser Lys Ile Leu Val Leu Arg Ser Val Glu Tyr Ala Arg Ala Phe Asn           
    210                 215                 220                           

gtg cca ctt cgc gta cgc tcg tct tat agt aat gat ccc ggc act ttg        720
Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Pro Gly Thr Leu           
225                 230                 235                 240           

att gcc ggc tct atg gag gat att cct gtg gaa gaa gca gtc ctt acc        768
Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr           
                245                 250                 255               

ggt gtc gca acc gac aag tcc gaa gcc aaa gta acc gtt ctg ggt att        816
Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Val Leu Gly Ile           
            260                 265                 270                   

tcc gat aag cca ggc gag gct gcg aag gtt ttc cgt gcg ttg gct gat        864
Ser Asp Lys Pro Gly Glu Ala Ala Lys Val Phe Arg Ala Leu Ala Asp           
        275                 280                 285                       

gca gaa atc aac att gac atg gtt ctg cag aac gtc tct tct gta gaa        912
Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Val Glu           
    290                 295                 300                           

gac ggc acc acc gac atc acc ttc acc tgc cct cgt tcc gac ggc cgc        960
Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ser Asp Gly Arg           
305                 310                 315                 320           

cgc gcg atg gag atc ttg aag aag ctt cag gtt cag ggc aac tgg acc       1008
Arg Ala Met Glu Ile Leu Lys Lys Leu Gln Val Gln Gly Asn Trp Thr           
                325                 330                 335               

aat gtg ctt tac gac gac cag gtc ggc aaa gtc tcc ctc gtg ggt gct       1056
Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala           
            340                 345                 350                   

ggc atg aag tct cac cca ggt gtt acc gca gag ttc atg gaa gct ctg       1104
Gly Met Lys Ser His Pro Gly Val Thr Ala Glu Phe Met Glu Ala Leu           
        355                 360                 365                       

cgc gat gtc aac gtg aac atc gaa ttg att tcc acc tct gag att cgt       1152
Arg Asp Val Asn Val Asn Ile Glu Leu Ile Ser Thr Ser Glu Ile Arg           
    370                 375                 380                           

att tcc gtg ctg atc cgt gaa gat gat ctg gat gct gct gca cgt gca       1200
Ile Ser Val Leu Ile Arg Glu Asp Asp Leu Asp Ala Ala Ala Arg Ala           
385                 390                 395                 400           

ttg cat gag cag ttc cag ctg ggc ggc gaa gac gaa gcc gtc gtt tat       1248
Leu His Glu Gln Phe Gln Leu Gly Gly Glu Asp Glu Ala Val Val Tyr           
                405                 410                 415               

gca ggc acc gga cgc taa                                               1266
Ala Gly Thr Gly Arg                                                       
            420                                                           


<210>  4
<211>  421
<212>  PRT
<213>  Corynebacterium glutamicum ATCC13032

<400>  4

Met Ala Leu Val Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Ser Ala 
1               5                   10                  15      


Glu Arg Ile Arg Asn Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala 
            20                  25                  30          


Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp 
        35                  40                  45              


Glu Leu Leu Glu Leu Ala Ala Ala Val Asn Pro Val Pro Pro Ala Arg 
    50                  55                  60                  


Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu 
65                  70                  75                  80  


Val Ala Met Ala Ile Glu Ser Leu Gly Ala Glu Ala Gln Ser Phe Thr 
                85                  90                  95      


Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg 
            100                 105                 110         


Ile Val Asp Val Thr Pro Gly Arg Val Arg Glu Ala Leu Asp Glu Gly 
        115                 120                 125             


Lys Ile Cys Ile Val Ala Gly Phe Gln Gly Val Asn Lys Glu Thr Arg 
    130                 135                 140                 


Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala 
145                 150                 155                 160 


Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val 
                165                 170                 175     


Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys 
            180                 185                 190         


Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly 
        195                 200                 205             


Ser Lys Ile Leu Val Leu Arg Ser Val Glu Tyr Ala Arg Ala Phe Asn 
    210                 215                 220                 


Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Pro Gly Thr Leu 
225                 230                 235                 240 


Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr 
                245                 250                 255     


Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Val Leu Gly Ile 
            260                 265                 270         


Ser Asp Lys Pro Gly Glu Ala Ala Lys Val Phe Arg Ala Leu Ala Asp 
        275                 280                 285             


Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Val Glu 
    290                 295                 300                 


Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ser Asp Gly Arg 
305                 310                 315                 320 


Arg Ala Met Glu Ile Leu Lys Lys Leu Gln Val Gln Gly Asn Trp Thr 
                325                 330                 335     


Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala 
            340                 345                 350         


Gly Met Lys Ser His Pro Gly Val Thr Ala Glu Phe Met Glu Ala Leu 
        355                 360                 365             


Arg Asp Val Asn Val Asn Ile Glu Leu Ile Ser Thr Ser Glu Ile Arg 
    370                 375                 380                 


Ile Ser Val Leu Ile Arg Glu Asp Asp Leu Asp Ala Ala Ala Arg Ala 
385                 390                 395                 400 


Leu His Glu Gln Phe Gln Leu Gly Gly Glu Asp Glu Ala Val Val Tyr 
                405                 410                 415     


Ala Gly Thr Gly Arg 
            420     


<210>  5
<211>  1631
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence comprising a deletion of the cds of the whiB4
       gene of Corynebacterium glutamicum ATCC13032 and the adjoining 
       stop codon


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  sequence required for Gibson assembly

<220>
<221>  misc_feature
<222>  (17)..(816)
<223>  sequence upstream of the site of deletion (5'-flanking sequence)

<220>
<221>  misc_feature
<222>  (811)..(822)
<223>  sequence attgtcttaatt

<220>
<221>  misc_feature
<222>  (816)..(817)
<223>  positions identifying the site of deletion of the coding sequence
       and the adjoining stop codon of the whiB4 gene

<220>
<221>  misc_feature
<222>  (817)..(1616)
<223>  sequence downstream of the site of deletion (3'-flanking 
       sequence)

<220>
<221>  misc_feature
<222>  (1617)..(1631)
<223>  sequence required for Gibson assembly

<400>  5
ggtacccggg gatcctgtct gcatcgtgtc attggtacgc gcaaccgcaa cgccagaaag       60

actagcgaaa ggcacaagtg cgagcgcacc aaggacgcca gcggcgactg tagatgcaac      120

gagctttgtc agagaattcg tggtggacac tccaacagcc tagcgataac cccgggaatc      180

gtgaacccaa gactccaaca atgtcagcca atctttattt attcgactgt gcgttactgc      240

tgacagtcgg catgctttgg gaatccacca ggcaagctat aggggactgt agagctgacg      300

ttcggaaaac gtgcagaaaa ccacgaactg cagtccggaa agcgccccac tggcccttcg      360

ccggcagcca cagaggggtt agaccattta ttcgcaactg tgcttacacc caagaattct      420

ccaacaagaa aaatccctct atcaacattt taagtgaatg accttaaggg tttgcggaat      480

gcgatccatg ttccaactcg cctcaagcgg tatgtgtgaa gcaggtttga agccagtgat      540

gacctctgat ggggttagtt tgtggcaatt tcggggctgg ctgaaatgcc taattttcca      600

aaactgaatt taatgagttg agctgtggta agactagtga actgcagata tgttggacaa      660

ttgtaaagtc ctctcgttgg aggcttggca gtgtggccat aaaagtattg cccccgttgg      720

tgtgatgcat tcgacagcaa attggctgtg tgactacact tgcgagtgta ttaagtatta      780

ggccgtgcat atgtagcgca ttttaaggag attgtcttaa tttcaagggc tggccattaa      840

cgtggtcggc ttttttgatt cagggcgcac cccaggcgca ccccaggcgc accccagcgc      900

accacaggcg caccccagcg caccacaggc gcaccagccc agcacaagag ccaacgcaag      960

tgtcaggcac gccagcaaag gggctagcaa gaagcacgcc cccgaagcct tagaaatgcg     1020

ttccccggta ggatgattgc catgactaaa tgggaatacg caactgtgcc tttgattacg     1080

catgcaacta agcagatcct cgatacttgg ggtgaggacg gctgggagtt ggtctcggtc     1140

atgcctggta tgaaccctga gaacctcgtt gcttacatga agcgtgaggt ggcttagttc     1200

ttatggcttc taattccgaa cgccttgcag agctgggcat ttctcttcct tccgttgcag     1260

cgcctgttgc tgcgtatgtt cctgcgattc agaccggtaa ccaggtgtgg acttctggtc     1320

agctgccttt cgttgatggt cagcttccgg ccaccggcaa ggttggcgct gaggtttccg     1380

ctgaggatgc ggagaagttg gctcgtgcgg ctgcgctaaa cgctcttgct gcgattgatg     1440

cgcttgttgg cattgataag gtcactcgcg ttttgaagat tgttggtttc gtggcgtctg     1500

ctgatgattt cagtggtcag cctgctgtcg tcaacggtgc ttccaatttg atgggtgagg     1560

ttttcggcga ggctggggcg catgcgcgtt ctgctgtggg cgtggcggag ttgccgagtc     1620

gacctgcagg c                                                          1631


<210>  6
<211>  1600
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleotide sequence comprising a deletion of part of the cds of 
       the whiB4 gene of Corynebacterium glutamicum ATCC13032


<220>
<221>  misc_feature
<222>  (1)..(800)
<223>  sequence upstream of the site of deletion (5'-flanking sequence)

<220>
<221>  CDS
<222>  (774)..(800)
<223>  residual 5'-coding sequence

<220>
<221>  misc_feature
<222>  (794)..(807)
<223>  sequence gcagcgcgccgggg

<220>
<221>  misc_feature
<222>  (800)..(801)
<223>  positions identfying the site of deletion within the coding 
       sequence of the whiB4 gene

<220>
<221>  CDS
<222>  (801)..(809)
<223>  residual 3'-coding sequence

<220>
<221>  misc_feature
<222>  (801)..(1600)
<223>  sequence downstream of the site of mutation (3'-flanking 
       sequence)

<220>
<221>  misc_feature
<222>  (810)..(812)
<223>  taa stop codon

<400>  6
accgcaacgc cagaaagact agcgaaaggc acaagtgcga gcgcaccaag gacgccagcg       60

gcgactgtag atgcaacgag ctttgtcaga gaattcgtgg tggacactcc aacagcctag      120

cgataacccc gggaatcgtg aacccaagac tccaacaatg tcagccaatc tttatttatt      180

cgactgtgcg ttactgctga cagtcggcat gctttgggaa tccaccaggc aagctatagg      240

ggactgtaga gctgacgttc ggaaaacgtg cagaaaacca cgaactgcag tccggaaagc      300

gccccactgg cccttcgccg gcagccacag aggggttaga ccatttattc gcaactgtgc      360

ttacacccaa gaattctcca acaagaaaaa tccctctatc aacattttaa gtgaatgacc      420

ttaagggttt gcggaatgcg atccatgttc caactcgcct caagcggtat gtgtgaagca      480

ggtttgaagc cagtgatgac ctctgatggg gttagtttgt ggcaatttcg gggctggctg      540

aaatgcctaa ttttccaaaa ctgaatttaa tgagttgagc tgtggtaaga ctagtgaact      600

gcagatatgt tggacaattg taaagtcctc tcgttggagg cttggcagtg tggccataaa      660

agtattgccc ccgttggtgt gatgcattcg acagcaaatt ggctgtgtga ctacacttgc      720

gagtgtatta agtattaggc cgtgcatatg tagcgcattt taaggagatt gtc atg         776
                                                           Met            
                                                           1              

acg tct gtg att cca gag cag cgc gcc ggg gtt taattaattt caagggctgg      829
Thr Ser Val Ile Pro Glu Gln Arg Ala Gly Val                               
            5                   10                                        

ccattaacgt ggtcggcttt tttgattcag ggcgcacccc aggcgcaccc caggcgcacc      889

ccagcgcacc acaggcgcac cccagcgcac cacaggcgca ccagcccagc acaagagcca      949

acgcaagtgt caggcacgcc agcaaagggg ctagcaagaa gcacgccccc gaagccttag     1009

aaatgcgttc cccggtagga tgattgccat gactaaatgg gaatacgcaa ctgtgccttt     1069

gattacgcat gcaactaagc agatcctcga tacttggggt gaggacggct gggagttggt     1129

ctcggtcatg cctggtatga accctgagaa cctcgttgct tacatgaagc gtgaggtggc     1189

ttagttctta tggcttctaa ttccgaacgc cttgcagagc tgggcatttc tcttccttcc     1249

gttgcagcgc ctgttgctgc gtatgttcct gcgattcaga ccggtaacca ggtgtggact     1309

tctggtcagc tgcctttcgt tgatggtcag cttccggcca ccggcaaggt tggcgctgag     1369

gtttccgctg aggatgcgga gaagttggct cgtgcggctg cgctaaacgc tcttgctgcg     1429

attgatgcgc ttgttggcat tgataaggtc actcgcgttt tgaagattgt tggtttcgtg     1489

gcgtctgctg atgatttcag tggtcagcct gctgtcgtca acggtgcttc caatttgatg     1549

ggtgaggttt tcggcgaggc tggggcgcat gcgcgttctg ctgtgggcgt g              1600


<210>  7
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  7

Met Thr Ser Val Ile Pro Glu Gln Arg 
1               5                   


<210>  8
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  primer 1f-NCgl0275

<400>  8
ggtacccggg gatcctgtct gcatcgtgtc attgg   


<210>  9
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(37)
<223>  primer 1r-NCgl0275

<400>  9
cccttgaaat taagacaatc tccttaaaat gcgctac


<210>  10
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  primer 2f-NCgl0275

<400>  10
taaggagatt gtcttaattt caagggctgg ccatt


<210>  11
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  primer 2f-NCgl0275_2

<400>  11
gcctgcaggt cgactcggca actccgccac gcc


<210>  12
<211>  829
<212>  DNA
<213>  artificial sequence

<220>
<223>  amplificate whiB4_up

<400>  12
ggtacccggg gatcctgtct gcatcgtgtc attggtacgc gcaaccgcaa cgccagaaag       60

actagcgaaa ggcacaagtg cgagcgcacc aaggacgcca gcggcgactg tagatgcaac      120

gagctttgtc agagaattcg tggtggacac tccaacagcc tagcgataac cccgggaatc      180

gtgaacccaa gactccaaca atgtcagcca atctttattt attcgactgt gcgttactgc      240

tgacagtcgg catgctttgg gaatccacca ggcaagctat aggggactgt agagctgacg      300

ttcggaaaac gtgcagaaaa ccacgaactg cagtccggaa agcgccccac tggcccttcg      360

ccggcagcca cagaggggtt agaccattta ttcgcaactg tgcttacacc caagaattct      420

ccaacaagaa aaatccctct atcaacattt taagtgaatg accttaaggg tttgcggaat      480

gcgatccatg ttccaactcg cctcaagcgg tatgtgtgaa gcaggtttga agccagtgat      540

gacctctgat ggggttagtt tgtggcaatt tcggggctgg ctgaaatgcc taattttcca      600

aaactgaatt taatgagttg agctgtggta agactagtga actgcagata tgttggacaa      660

ttgtaaagtc ctctcgttgg aggcttggca gtgtggccat aaaagtattg cccccgttgg      720

tgtgatgcat tcgacagcaa attggctgtg tgactacact tgcgagtgta ttaagtatta      780

ggccgtgcat atgtagcgca ttttaaggag attgtcttaa tttcaaggg                  829


<210>  13
<211>  828
<212>  DNA
<213>  artificial sequence

<220>
<223>  amplificate whiB4_down

<400>  13
taaggagatt gtcttaattt caagggctgg ccattaacgt ggtcggcttt tttgattcag       60

ggcgcacccc aggcgcaccc caggcgcacc ccagcgcacc acaggcgcac cccagcgcac      120

cacaggcgca ccagcccagc acaagagcca acgcaagtgt caggcacgcc agcaaagggg      180

ctagcaagaa gcacgccccc gaagccttag aaatgcgttc cccggtagga tgattgccat      240

gactaaatgg gaatacgcaa ctgtgccttt gattacgcat gcaactaagc agatcctcga      300

tacttggggt gaggacggct gggagttggt ctcggtcatg cctggtatga accctgagaa      360

cctcgttgct tacatgaagc gtgaggtggc ttagttctta tggcttctaa ttccgaacgc      420

cttgcagagc tgggcatttc tcttccttcc gttgcagcgc ctgttgctgc gtatgttcct      480

gcgattcaga ccggtaacca ggtgtggact tctggtcagc tgcctttcgt tgatggtcag      540

cttccggcca ccggcaaggt tggcgctgag gtttccgctg aggatgcgga gaagttggct      600

cgtgcggctg cgctaaacgc tcttgctgcg attgatgcgc ttgttggcat tgataaggtc      660

actcgcgttt tgaagattgt tggtttcgtg gcgtctgctg atgatttcag tggtcagcct      720

gctgtcgtca acggtgcttc caatttgatg ggtgaggttt tcggcgaggc tggggcgcat      780

gcgcgttctg ctgtgggcgt ggcggagttg ccgagtcgac ctgcaggc                   828


<210>  14
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  primer DNCgl0275_seq_1

<400>  14
gccagtgatg acctctgatg


<210>  15
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  primer DNCgl0275_seq_2

<400>  15
ttccgaacgc cttgcagagc


<210>  16
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  primer pCV22_1.p

<400>  16
aggtttcccg actggaaagc


<210>  17
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  primer Ncgl0275_rev

<400>  17
gtggcggcaa attgctgctg


<210>  18
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  primer NCgl0276_rev2

<400>  18
ataccaggca tgaccgagac


<210>  19
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  primer Ncgl0274_fw

<400>  19
tgcaacgagc tttgtcagag