                         SEQUENCE LISTING

<110>  BEIJING MEIKANG GENO-IMMUNE BIOTECHNOLOGY CO., LTD.
 
<120>  CD19- AND PSMA-BASED COMBINED CAR-T IMMUNOTHERAPY

<130>  PCT190359PPC

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  the single-chain antibody against CD19

<400>  1

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 
            100                 105                 110         


Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 
        115                 120                 125             


Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 
    130                 135                 140                 


Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 
145                 150                 155                 160 


Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
                165                 170                 175     


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 
            180                 185                 190         


Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 
        195                 200                 205             


Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 
    210                 215                 220                 


Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  2
<211>  240
<212>  PRT
<213>  Artificial sequence

<220>
<223>  the single-chain antibody against PSMA

<400>  2

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ile Ser Gly Tyr Thr Phe Thr Glu Tyr 
            20                  25                  30          


Thr Ile His Trp Val Lys Gln Ala Ser Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Glu Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
            100                 105                 110         


Val Ser Ser Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Ser Glu 
        115                 120                 125             


Gly Ser Thr Lys Gly Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu 
    130                 135                 140                 


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Ile Cys Lys Ala Ser Gln 
145                 150                 155                 160 


Asp Val Gly Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
                165                 170                 175     


Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro 
            180                 185                 190         


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
        195                 200                 205             


Ser Ser Leu Gln Pro Glu Asp Phe Ala Asp Tyr Phe Cys Gln Gln Tyr 
    210                 215                 220                 


Asn Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
225                 230                 235                 240 


<210>  3
<211>  423
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Caspase 9

<400>  3

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro Met Gly Val Gln Val Glu Thr Ile Ser Pro 
            20                  25                  30          


Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His 
        35                  40                  45              


Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp 
    50                  55                  60                  


Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg 
65                  70                  75                  80  


Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys 
                85                  90                  95      


Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly 
            100                 105                 110         


Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys 
        115                 120                 125             


Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ala Met Val 
    130                 135                 140                 


Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu 
145                 150                 155                 160 


Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe 
                165                 170                 175     


Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys 
            180                 185                 190         


Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met Val Glu Val 
        195                 200                 205             


Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu 
    210                 215                 220                 


Ala Arg Gln Asp His Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu 
225                 230                 235                 240 


Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr 
                245                 250                 255     


Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe 
            260                 265                 270         


Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe 
        275                 280                 285             


Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala 
    290                 295                 300                 


Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp 
305                 310                 315                 320 


Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala 
                325                 330                 335     


Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr 
            340                 345                 350         


Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr 
        355                 360                 365             


Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp 
    370                 375                 380                 


Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly 
385                 390                 395                 400 


Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu 
                405                 410                 415     


Phe Phe Lys Thr Ser Ala Ser 
            420             


<210>  4
<211>  986
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory-CD19 scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  4

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asn Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro 
65                  70                  75                  80  


Thr Tyr Ala Asp Ala Phe Lys Gly Arg Val Thr Met Thr Thr Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Gly Asp Tyr Gly Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ser Thr Ser 
    130                 135                 140                 


Gly Ser Gly Lys Pro Gly Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile 
145                 150                 155                 160 


Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg 
                165                 170                 175     


Ala Thr Ile Asn Cys Arg Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr 
            180                 185                 190         


Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu 
        195                 200                 205             


Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
225                 230                 235                 240 


Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln His Ser Arg Glu Val 
                245                 250                 255     


Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala Ala Ala 
            260                 265                 270         


Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
        275                 280                 285             


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
    290                 295                 300                 


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
305                 310                 315                 320 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                325                 330                 335     


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            340                 345                 350         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        355                 360                 365             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Gly Gly Gly Gly Ser Gln Arg Arg Lys Tyr Arg Ser Asn Lys 
385                 390                 395                 400 


Gly Glu Ser Pro Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro 
                405                 410                 415     


Arg Glu Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys 
            420                 425                 430         


Pro Glu Pro Ala Cys Ser Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        435                 440                 445             


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
    450                 455                 460                 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
465                 470                 475                 480 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
                485                 490                 495     


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
            500                 505                 510         


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
        515                 520                 525             


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
    530                 535                 540                 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
545                 550                 555                 560 


Arg Thr Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
                565                 570                 575     


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly Val Gln Val Glu Thr 
            580                 585                 590         


Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys 
        595                 600                 605             


Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser 
    610                 615                 620                 


Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu 
625                 630                 635                 640 


Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln 
                645                 650                 655     


Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly 
            660                 665                 670         


His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu 
        675                 680                 685             


Leu Leu Lys Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    690                 695                 700                 


Ala Met Val Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala 
705                 710                 715                 720 


Tyr Ile Leu Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn 
                725                 730                 735     


Val Asn Phe Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn 
            740                 745                 750         


Ile Asp Cys Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met 
        755                 760                 765             


Val Glu Val Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu 
    770                 775                 780                 


Leu Glu Leu Ala Arg Gln Asp His Gly Ala Leu Asp Cys Cys Val Val 
785                 790                 795                 800 


Val Ile Leu Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly 
                805                 810                 815     


Ala Val Tyr Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val 
            820                 825                 830         


Asn Ile Phe Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys 
        835                 840                 845             


Leu Phe Phe Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe 
    850                 855                 860                 


Glu Val Ala Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro 
865                 870                 875                 880 


Glu Pro Asp Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln 
                885                 890                 895     


Leu Asp Ala Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser 
            900                 905                 910         


Tyr Ser Thr Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly 
        915                 920                 925             


Ser Trp Tyr Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His 
    930                 935                 940                 


Ser Glu Asp Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser 
945                 950                 955                 960 


Val Lys Gly Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg 
                965                 970                 975     


Lys Lys Leu Phe Phe Lys Thr Ser Ala Ser 
            980                 985     


<210>  5
<211>  2955
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Secretory-CD19 scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  5
atgctgctgc tggtcacaag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg     60

atccccgaca tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgacaga    120

gtgaccatca gctgccgggc cagccaggac atcagcaagt acctgaactg gtatcagcag    180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gccggctgca cagcggcgtg    240

cccagcagat tttctggcag cggatctggc accgactaca gcctgaccat ctccaacctg    300

gaacaggaag atatcgctac ctacttctgt cagcagggca acaccctgcc ctacaccttc    360

ggcggaggca ccaagctgga aatcaccggc agcaccagcg gctccggcaa gcctggatct    420

ggcgagggca gcaccaaggg cgaagtgaag ctgcaggaaa gcggccctgg cctggtcgcc    480

cctagccaga gcctgtccgt gacctgtacc gtgtccggcg tgtccctgcc cgactacggc    540

gtgtcctgga tcagacagcc ccccagaaag ggcctggaat ggctgggcgt gatctggggc    600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac    660

agcaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac    720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc    780

accagcgtga cagtctcttc tgcggccgca attgaagtta tgtatcctcc tccttaccta    840

gacaatgaga agagcaatgg aaccattatc catgtgaaag ggaaacacct ttgtccaagt    900

cccctatttc ccggaccttc taagcccttt tgggtgctgg tggtggttgg gggagtcctg    960

gcttgctata gcttgctagt aacagtggcc tttattattt tctgggtgag gagtaagagg   1020

agcaggctcc tgcacagtga ctacatgaac atgactcccc gccgccctgg gcccacccgc   1080

aagcattacc agccctatgc cccaccacgc gacttcgcag cctatcgctc cgctagcgga   1140

ggtggaggtt ctggaggtgg tggaagtcaa agaaggaagt accgcagcaa caaaggagaa   1200

tctcccgtcg agccagccga gccctgtcat tattcatgcc caagggagga ggagggaagt   1260

acaatcccaa ttcaagaaga ctacaggaag cccgaacctg catgcagtcc aggtggaggc   1320

ggttctggag gcggtggctc ccgggtgaaa ttctcacggt ctgcagacgc acccgcttac   1380

cagcaaggcc agaaccaact ctataacgag ctcaatctag gacgaagaga ggagtacgat   1440

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgag aaggaagaac   1500

cctcaggaag gcctgtacaa tgaactgcag aaagataaga tggcggaggc ctacagtgag   1560

attgggatga aaggcgagcg ccggaggggc aaggggcacg atggccttta ccagggtctc   1620

agtacagcca ccaaggacac ctacgacgcc cttcacatgc aggccctgcc ccctcgcact   1680

agtggctccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac   1740

cccggtccca tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc   1800

aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtg   1860

gactcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc   1920

cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata   1980

tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact   2040

ctcgtcttcg atgtggagct tctaaaactg gaaggtggag gcggttcagg cggcggcggc   2100

agcggcgcca tggtcggtgc tcttgagagt ttgaggggaa atgcagattt ggcttacatc   2160

ctgagcatgg agccctgtgg ccactgcctc attatcaaca atgtgaactt ctgccgtgag   2220

tccgggctcc gcacccgcac tggctccaac atcgactgtg agaagttgcg gcgtcgcttc   2280

tcctcgctgc atttcatggt ggaggtgaag ggcgacctga ctgccaagaa aatggtgctg   2340

gctttgctgg agctggcgcg gcaggaccac ggtgctctgg actgctgcgt ggtggtcatt   2400

ctctctcacg gctgtcaggc cagccacctg cagttcccag gggctgtcta cggcacagat   2460

ggatgccctg tgtcggtcga gaagattgtg aacatcttca atgggaccag ctgccccagc   2520

ctgggaggga agcccaagct ctttttcatc caggcctgtg gtggggagca gaaagaccat   2580

gggtttgagg tggcctccac ttcccctgaa gacgagtccc ctggcagtaa ccccgagcca   2640

gatgccaccc cgttccagga aggtttgagg accttcgacc agctggacgc catatctagt   2700

ttgcccacac ccagtgacat ctttgtgtcc tactctactt tcccaggttt tgtttcctgg   2760

agggacccca agagtggctc ctggtacgtt gagaccctgg acgacatctt tgagcagtgg   2820

gctcactctg aagacctgca gtccctcctg cttagggtcg ctaatgctgt ttcggtgaaa   2880

gggatttata aacagatgcc tggttgcttt aatttcctcc ggaaaaaact tttctttaaa   2940

acatcagcta gttaa                                                    2955


<210>  6
<211>  978
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory-PSMA scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  6

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ile Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Glu Tyr Thr Ile His Trp Val Lys Gln Ala Ser Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Glu Asp Arg Ala Thr Leu Thr Val Asp Lys Ser 
                85                  90                  95      


Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Thr Val Thr Val Ser Ser Gly Ser Thr Ser Gly Ser Gly Lys 
    130                 135                 140                 


Pro Gly Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile Val Met Thr Gln 
145                 150                 155                 160 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Ile 
                165                 170                 175     


Cys Lys Ala Ser Gln Asp Val Gly Thr Ala Val Asp Trp Tyr Gln Gln 
            180                 185                 190         


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg 
        195                 200                 205             


His Thr Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Asp Tyr 
225                 230                 235                 240 


Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Ala Ala Ala Ile Glu Val Met Tyr Pro Pro Pro 
            260                 265                 270         


Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly 
        275                 280                 285             


Lys His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe 
    290                 295                 300                 


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
305                 310                 315                 320 


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
                325                 330                 335     


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
            340                 345                 350         


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
        355                 360                 365             


Tyr Arg Ser Ala Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln 
    370                 375                 380                 


Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro Ala 
385                 390                 395                 400 


Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr Ile 
                405                 410                 415     


Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro Gly 
            420                 425                 430         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Val Lys Phe Ser Arg Ser 
        435                 440                 445             


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    450                 455                 460                 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
465                 470                 475                 480 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                485                 490                 495     


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            500                 505                 510         


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        515                 520                 525             


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    530                 535                 540                 


Leu His Met Gln Ala Leu Pro Pro Arg Thr Ser Gly Ser Gly Ala Thr 
545                 550                 555                 560 


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
                565                 570                 575     


Pro Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
            580                 585                 590         


Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
        595                 600                 605             


Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
    610                 615                 620                 


Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
625                 630                 635                 640 


Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
                645                 650                 655     


Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
            660                 665                 670         


Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Gly Gly 
        675                 680                 685             


Gly Ser Gly Gly Gly Gly Ser Gly Ala Met Val Gly Ala Leu Glu Ser 
    690                 695                 700                 


Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys 
705                 710                 715                 720 


Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly 
                725                 730                 735     


Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg 
            740                 745                 750         


Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr 
        755                 760                 765             


Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Arg Gln Asp His 
    770                 775                 780                 


Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln 
785                 790                 795                 800 


Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys 
                805                 810                 815     


Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys 
            820                 825                 830         


Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly 
        835                 840                 845             


Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu 
    850                 855                 860                 


Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln 
865                 870                 875                 880 


Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro 
                885                 890                 895     


Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val 
            900                 905                 910         


Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp 
        915                 920                 925             


Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu 
    930                 935                 940                 


Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met 
945                 950                 955                 960 


Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser 
                965                 970                 975     


Ala Ser 
        


<210>  7
<211>  2937
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Secretory-PSMA scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  7
atggccctcc ctgttaccgc tctgctcctc cccttggccc ttctgctgca tgcagccaga     60

cctgaggtcc aactggtgca gtctggtgct gaagtgaaga agcctggtgc cagcgtgaag    120

attagctgca agattagcgg ctacaccttc accgagtata ccattcactg ggtcaaacaa    180

gcctctggaa aagggctgga atggataggc aatatcaacc ccaacaatgg cggaacaacc    240

tacaaccaaa agtttgagga tcgtgccact ctgacggttg acaagtccac gagcacagcc    300

tacatggagc tgtcaagtct gaggtccgag gatactgcgg tctactattg tgctgctggg    360

tggaacttcg actattgggg acagggtacg acagttactg tgtcctcagg ctcaacatcc    420

ggctctggaa agcctgggtc cagtgaaggg agcacaaaag gggacatcgt gatgacccag    480

tctccaagca gccttagcgc tagtgtaggg gatcgagtga ccatcatctg caaagcatct    540

caggacgtag gcactgcagt ggattggtat cagcagaaac caggaaaagc gccgaaactg    600

ttgatctact gggcaagtac acgccacact ggagtcccag atcggtttac cgggtccggc    660

tcaggcactg acttcactct gaccatttcc tctcttcagc ccgaagattt tgccgactac    720

ttctgtcagc agtacaatag ctatcccctg acatttggcg gtggaacaaa gctcgagata    780

aaggcggccg caattgaagt tatgtatcct cctccttacc tagacaatga gaagagcaat    840

ggaaccatta tccatgtgaa agggaaacac ctttgtccaa gtcccctatt tcccggacct    900

tctaagccct tttgggtgct ggtggtggtt gggggagtcc tggcttgcta tagcttgcta    960

gtaacagtgg cctttattat tttctgggtg aggagtaaga ggagcaggct cctgcacagt   1020

gactacatga acatgactcc ccgccgccct gggcccaccc gcaagcatta ccagccctat   1080

gccccaccac gcgacttcgc agcctatcgc tccgctagcg gaggtggagg ttctggaggt   1140

ggtggaagtc aaagaaggaa gtaccgcagc aacaaaggag aatctcccgt cgagccagcc   1200

gagccctgtc attattcatg cccaagggag gaggagggaa gtacaatccc aattcaagaa   1260

gactacagga agcccgaacc tgcatgcagt ccaggtggag gcggttctgg aggcggtggc   1320

tcccgggtga aattctcacg gtctgcagac gcacccgctt accagcaagg ccagaaccaa   1380

ctctataacg agctcaatct aggacgaaga gaggagtacg atgttttgga caagagacgt   1440

ggccgggacc ctgagatggg gggaaagccg agaaggaaga accctcagga aggcctgtac   1500

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag   1560

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac   1620

acctacgacg cccttcacat gcaggccctg ccccctcgca ctagtggctc cggagccacg   1680

aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc catgggagtg   1740

caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc   1800

gtggtgcact acaccgggat gcttgaagat ggaaagaaag tggactcctc ccgggacaga   1860

aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg   1920

gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat   1980

ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag   2040

cttctaaaac tggaaggtgg aggcggttca ggcggcggcg gcagcggcgc catggtcggt   2100

gctcttgaga gtttgagggg aaatgcagat ttggcttaca tcctgagcat ggagccctgt   2160

ggccactgcc tcattatcaa caatgtgaac ttctgccgtg agtccgggct ccgcacccgc   2220

actggctcca acatcgactg tgagaagttg cggcgtcgct tctcctcgct gcatttcatg   2280

gtggaggtga agggcgacct gactgccaag aaaatggtgc tggctttgct ggagctggcg   2340

cggcaggacc acggtgctct ggactgctgc gtggtggtca ttctctctca cggctgtcag   2400

gccagccacc tgcagttccc aggggctgtc tacggcacag atggatgccc tgtgtcggtc   2460

gagaagattg tgaacatctt caatgggacc agctgcccca gcctgggagg gaagcccaag   2520

ctctttttca tccaggcctg tggtggggag cagaaagacc atgggtttga ggtggcctcc   2580

acttcccctg aagacgagtc ccctggcagt aaccccgagc cagatgccac cccgttccag   2640

gaaggtttga ggaccttcga ccagctggac gccatatcta gtttgcccac acccagtgac   2700

atctttgtgt cctactctac tttcccaggt tttgtttcct ggagggaccc caagagtggc   2760

tcctggtacg ttgagaccct ggacgacatc tttgagcagt gggctcactc tgaagacctg   2820

cagtccctcc tgcttagggt cgctaatgct gtttcggtga aagggattta taaacagatg   2880

cctggttgct ttaatttcct ccggaaaaaa cttttcttta aaacatcagc tagttaa      2937


<210>  8
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory signal peptide

<400>  8

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  9
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory signal peptide

<400>  9

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


