                         SEQUENCE LISTING

<110>  BEIJING MEIKANG GENO-IMMUNE BIOTECHNOLOGY CO., LTD.
 
<120>  CD19- AND CD70-BASED COMBINED CAR-T IMMUNOTHERAPY

<130>  PCT190356PPC

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  the single chain antibody against CD19

<400>  1

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 
            100                 105                 110         


Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 
        115                 120                 125             


Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 
    130                 135                 140                 


Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 
145                 150                 155                 160 


Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
                165                 170                 175     


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 
            180                 185                 190         


Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 
        195                 200                 205             


Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 
    210                 215                 220                 


Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  2
<211>  247
<212>  PRT
<213>  Artificial sequence

<220>
<223>  the single chain antibody against CD70

<400>  2

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Ala Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Met Thr Thr Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Tyr Gly Asp Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly 
        115                 120                 125             


Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile Val Met Thr Gln Ser Pro 
    130                 135                 140                 


Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Arg 
145                 150                 155                 160 


Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr Ser Phe Met His Trp Tyr 
                165                 170                 175     


Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Leu Ala Ser 
            180                 185                 190         


Asn Leu Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala 
    210                 215                 220                 


Val Tyr Tyr Cys Gln His Ser Arg Glu Val Pro Trp Thr Phe Gly Gln 
225                 230                 235                 240 


Gly Thr Lys Val Glu Ile Lys 
                245         


<210>  3
<211>  423
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Caspase 9

<400>  3

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro Met Gly Val Gln Val Glu Thr Ile Ser Pro 
            20                  25                  30          


Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His 
        35                  40                  45              


Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp 
    50                  55                  60                  


Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg 
65                  70                  75                  80  


Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys 
                85                  90                  95      


Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly 
            100                 105                 110         


Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys 
        115                 120                 125             


Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ala Met Val 
    130                 135                 140                 


Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu 
145                 150                 155                 160 


Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe 
                165                 170                 175     


Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys 
            180                 185                 190         


Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met Val Glu Val 
        195                 200                 205             


Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu 
    210                 215                 220                 


Ala Arg Gln Asp His Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu 
225                 230                 235                 240 


Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr 
                245                 250                 255     


Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe 
            260                 265                 270         


Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe 
        275                 280                 285             


Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala 
    290                 295                 300                 


Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp 
305                 310                 315                 320 


Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala 
                325                 330                 335     


Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr 
            340                 345                 350         


Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr 
        355                 360                 365             


Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp 
    370                 375                 380                 


Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly 
385                 390                 395                 400 


Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu 
                405                 410                 415     


Phe Phe Lys Thr Ser Ala Ser 
            420             


<210>  4
<211>  986
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory-CD19 scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  4

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asn Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro 
65                  70                  75                  80  


Thr Tyr Ala Asp Ala Phe Lys Gly Arg Val Thr Met Thr Thr Asp Thr 
                85                  90                  95      


Ser Thr Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Asp Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Gly Asp Tyr Gly Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ser Thr Ser 
    130                 135                 140                 


Gly Ser Gly Lys Pro Gly Ser Ser Glu Gly Ser Thr Lys Gly Asp Ile 
145                 150                 155                 160 


Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg 
                165                 170                 175     


Ala Thr Ile Asn Cys Arg Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr 
            180                 185                 190         


Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu 
        195                 200                 205             


Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
225                 230                 235                 240 


Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln His Ser Arg Glu Val 
                245                 250                 255     


Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala Ala Ala 
            260                 265                 270         


Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
        275                 280                 285             


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
    290                 295                 300                 


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
305                 310                 315                 320 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                325                 330                 335     


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            340                 345                 350         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        355                 360                 365             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Gly Gly Gly 
    370                 375                 380                 


Gly Ser Gly Gly Gly Gly Ser Gln Arg Arg Lys Tyr Arg Ser Asn Lys 
385                 390                 395                 400 


Gly Glu Ser Pro Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro 
                405                 410                 415     


Arg Glu Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys 
            420                 425                 430         


Pro Glu Pro Ala Cys Ser Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        435                 440                 445             


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
    450                 455                 460                 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
465                 470                 475                 480 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
                485                 490                 495     


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
            500                 505                 510         


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
        515                 520                 525             


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
    530                 535                 540                 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
545                 550                 555                 560 


Arg Thr Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
                565                 570                 575     


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly Val Gln Val Glu Thr 
            580                 585                 590         


Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys 
        595                 600                 605             


Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser 
    610                 615                 620                 


Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu 
625                 630                 635                 640 


Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln 
                645                 650                 655     


Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly 
            660                 665                 670         


His Pro Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu 
        675                 680                 685             


Leu Leu Lys Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    690                 695                 700                 


Ala Met Val Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala 
705                 710                 715                 720 


Tyr Ile Leu Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn 
                725                 730                 735     


Val Asn Phe Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn 
            740                 745                 750         


Ile Asp Cys Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met 
        755                 760                 765             


Val Glu Val Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu 
    770                 775                 780                 


Leu Glu Leu Ala Arg Gln Asp His Gly Ala Leu Asp Cys Cys Val Val 
785                 790                 795                 800 


Val Ile Leu Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly 
                805                 810                 815     


Ala Val Tyr Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val 
            820                 825                 830         


Asn Ile Phe Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys 
        835                 840                 845             


Leu Phe Phe Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe 
    850                 855                 860                 


Glu Val Ala Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro 
865                 870                 875                 880 


Glu Pro Asp Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln 
                885                 890                 895     


Leu Asp Ala Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser 
            900                 905                 910         


Tyr Ser Thr Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly 
        915                 920                 925             


Ser Trp Tyr Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His 
    930                 935                 940                 


Ser Glu Asp Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser 
945                 950                 955                 960 


Val Lys Gly Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg 
                965                 970                 975     


Lys Lys Leu Phe Phe Lys Thr Ser Ala Ser 
            980                 985     


<210>  5
<211>  2955
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Secretory-CD19 scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  5
atgctgctgc tggtcacaag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg     60

atccccgaca tccagatgac ccagaccacc agcagcctga gcgccagcct gggcgacaga    120

gtgaccatca gctgccgggc cagccaggac atcagcaagt acctgaactg gtatcagcag    180

aaacccgacg gcaccgtgaa gctgctgatc taccacacca gccggctgca cagcggcgtg    240

cccagcagat tttctggcag cggatctggc accgactaca gcctgaccat ctccaacctg    300

gaacaggaag atatcgctac ctacttctgt cagcagggca acaccctgcc ctacaccttc    360

ggcggaggca ccaagctgga aatcaccggc agcaccagcg gctccggcaa gcctggatct    420

ggcgagggca gcaccaaggg cgaagtgaag ctgcaggaaa gcggccctgg cctggtcgcc    480

cctagccaga gcctgtccgt gacctgtacc gtgtccggcg tgtccctgcc cgactacggc    540

gtgtcctgga tcagacagcc ccccagaaag ggcctggaat ggctgggcgt gatctggggc    600

agcgagacaa cctactacaa cagcgccctg aagtcccggc tgaccatcat caaggacaac    660

agcaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac    720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc    780

accagcgtga cagtctcttc tgcggccgca attgaagtta tgtatcctcc tccttaccta    840

gacaatgaga agagcaatgg aaccattatc catgtgaaag ggaaacacct ttgtccaagt    900

cccctatttc ccggaccttc taagcccttt tgggtgctgg tggtggttgg gggagtcctg    960

gcttgctata gcttgctagt aacagtggcc tttattattt tctgggtgag gagtaagagg   1020

agcaggctcc tgcacagtga ctacatgaac atgactcccc gccgccctgg gcccacccgc   1080

aagcattacc agccctatgc cccaccacgc gacttcgcag cctatcgctc cgctagcgga   1140

ggtggaggtt ctggaggtgg tggaagtcaa agaaggaagt accgcagcaa caaaggagaa   1200

tctcccgtcg agccagccga gccctgtcat tattcatgcc caagggagga ggagggaagt   1260

acaatcccaa ttcaagaaga ctacaggaag cccgaacctg catgcagtcc aggtggaggc   1320

ggttctggag gcggtggctc ccgggtgaaa ttctcacggt ctgcagacgc acccgcttac   1380

cagcaaggcc agaaccaact ctataacgag ctcaatctag gacgaagaga ggagtacgat   1440

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgag aaggaagaac   1500

cctcaggaag gcctgtacaa tgaactgcag aaagataaga tggcggaggc ctacagtgag   1560

attgggatga aaggcgagcg ccggaggggc aaggggcacg atggccttta ccagggtctc   1620

agtacagcca ccaaggacac ctacgacgcc cttcacatgc aggccctgcc ccctcgcact   1680

agtggctccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac   1740

cccggtccca tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc   1800

aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtg   1860

gactcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc   1920

cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata   1980

tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact   2040

ctcgtcttcg atgtggagct tctaaaactg gaaggtggag gcggttcagg cggcggcggc   2100

agcggcgcca tggtcggtgc tcttgagagt ttgaggggaa atgcagattt ggcttacatc   2160

ctgagcatgg agccctgtgg ccactgcctc attatcaaca atgtgaactt ctgccgtgag   2220

tccgggctcc gcacccgcac tggctccaac atcgactgtg agaagttgcg gcgtcgcttc   2280

tcctcgctgc atttcatggt ggaggtgaag ggcgacctga ctgccaagaa aatggtgctg   2340

gctttgctgg agctggcgcg gcaggaccac ggtgctctgg actgctgcgt ggtggtcatt   2400

ctctctcacg gctgtcaggc cagccacctg cagttcccag gggctgtcta cggcacagat   2460

ggatgccctg tgtcggtcga gaagattgtg aacatcttca atgggaccag ctgccccagc   2520

ctgggaggga agcccaagct ctttttcatc caggcctgtg gtggggagca gaaagaccat   2580

gggtttgagg tggcctccac ttcccctgaa gacgagtccc ctggcagtaa ccccgagcca   2640

gatgccaccc cgttccagga aggtttgagg accttcgacc agctggacgc catatctagt   2700

ttgcccacac ccagtgacat ctttgtgtcc tactctactt tcccaggttt tgtttcctgg   2760

agggacccca agagtggctc ctggtacgtt gagaccctgg acgacatctt tgagcagtgg   2820

gctcactctg aagacctgca gtccctcctg cttagggtcg ctaatgctgt ttcggtgaaa   2880

gggatttata aacagatgcc tggttgcttt aatttcctcc ggaaaaaact tttctttaaa   2940

acatcagcta gttaa                                                    2955


<210>  6
<211>  984
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory-CD70 scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  6

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly 
    50                  55                  60                  


Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr 
                85                  90                  95      


Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 
            100                 105                 110         


Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
        115                 120                 125             


Thr Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser 
    130                 135                 140                 


Thr Lys Gly Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala 
145                 150                 155                 160 


Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu 
                165                 170                 175     


Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu 
            180                 185                 190         


Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser 
        195                 200                 205             


Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln 
    210                 215                 220                 


Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 
225                 230                 235                 240 


Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly Thr 
        275                 280                 285             


Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe Pro 
    290                 295                 300                 


Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
305                 310                 315                 320 


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
                325                 330                 335     


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
            340                 345                 350         


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
        355                 360                 365             


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Ser Gly Gly Gly Gly Ser 
    370                 375                 380                 


Gly Gly Gly Gly Ser Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu 
385                 390                 395                 400 


Ser Pro Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu 
                405                 410                 415     


Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu 
            420                 425                 430         


Pro Ala Cys Ser Pro Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg 
        435                 440                 445             


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
    450                 455                 460                 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
465                 470                 475                 480 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
                485                 490                 495     


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
            500                 505                 510         


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
        515                 520                 525             


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
    530                 535                 540                 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Thr 
545                 550                 555                 560 


Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
                565                 570                 575     


Val Glu Glu Asn Pro Gly Pro Met Gly Val Gln Val Glu Thr Ile Ser 
            580                 585                 590         


Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val 
        595                 600                 605             


His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg 
    610                 615                 620                 


Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile 
625                 630                 635                 640 


Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala 
                645                 650                 655     


Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro 
            660                 665                 670         


Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu 
        675                 680                 685             


Lys Leu Glu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Ala Met 
    690                 695                 700                 


Val Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile 
705                 710                 715                 720 


Leu Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn Val Asn 
                725                 730                 735     


Phe Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp 
            740                 745                 750         


Cys Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met Val Glu 
        755                 760                 765             


Val Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu Leu Glu 
    770                 775                 780                 


Leu Ala Arg Gln Asp His Gly Ala Leu Asp Cys Cys Val Val Val Ile 
785                 790                 795                 800 


Leu Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly Ala Val 
                805                 810                 815     


Tyr Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val Asn Ile 
            820                 825                 830         


Phe Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe 
        835                 840                 845             


Phe Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe Glu Val 
    850                 855                 860                 


Ala Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro 
865                 870                 875                 880 


Asp Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp 
                885                 890                 895     


Ala Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser 
            900                 905                 910         


Thr Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp 
        915                 920                 925             


Tyr Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His Ser Glu 
    930                 935                 940                 


Asp Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser Val Lys 
945                 950                 955                 960 


Gly Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys 
                965                 970                 975     


Leu Phe Phe Lys Thr Ser Ala Ser 
            980                 


<210>  7
<211>  2961
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Secretory-CD70 scFv-CD28-CD27-CD3ζ-2A-FBKP.Casp9

<400>  7
atgctgctgc tcgttacatc actcctcctg tgtgaactcc ctcacccagc cttcctcctg     60

atccctcagg ttcagctcgt gcagtccgga gccgaagtta agaaaccagg agcttcagtt    120

aaagtgtcat gtaaagcctc cggatacacc tttaccaact atggtatgaa ctgggttaga    180

caagctcctg gacaaggtct ggagtggatg ggttggatca acacttacac tggagagcca    240

acctacgcag atgcctttaa gggcagggtt accatgacta ccgatacttc cacctccact    300

gcatacatgg agctgagatc cctcaggtcc gacgatacag ccgtctatta ctgtgctagg    360

gattatggag attacggcat ggattactgg ggacaaggta ccacagtgac cgtctcttca    420

ggcagtactt caggatctgg taagcctgga agctccgaag gcagtaccaa gggagacatc    480

gttatgacac agtctccaga ttctctcgca gtcagtctgg gagaaagggc aaccatcaac    540

tgtagagcca gcaagtcagt cagcacctct ggctactctt tcatgcactg gtatcagcag    600

aaacctggac agccaccaaa gctcctcatc tacctggcct caaatctgga gagtggcgtg    660

cctgatcgct ttagtggaag cggttcaggg acagacttca cactgaccat tagtagcctg    720

caagccgagg atgtcgcagt gtactactgc cagcacagca gagaggtgcc ttggaccttt    780

gggcagggaa ctaaggtcga aatcaaagcg gccgcaattg aagttatgta tcctcctcct    840

tacctagaca atgagaagag caatggaacc attatccatg tgaaagggaa acacctttgt    900

ccaagtcccc tatttcccgg accttctaag cccttttggg tgctggtggt ggttggggga    960

gtcctggctt gctatagctt gctagtaaca gtggccttta ttattttctg ggtgaggagt   1020

aagaggagca ggctcctgca cagtgactac atgaacatga ctccccgccg ccctgggccc   1080

acccgcaagc attaccagcc ctatgcccca ccacgcgact tcgcagccta tcgctccgct   1140

agcggaggtg gaggttctgg aggtggtgga agtcaaagaa ggaagtaccg cagcaacaaa   1200

ggagaatctc ccgtcgagcc agccgagccc tgtcattatt catgcccaag ggaggaggag   1260

ggaagtacaa tcccaattca agaagactac aggaagcccg aacctgcatg cagtccaggt   1320

ggaggcggtt ctggaggcgg tggctcccgg gtgaaattct cacggtctgc agacgcaccc   1380

gcttaccagc aaggccagaa ccaactctat aacgagctca atctaggacg aagagaggag   1440

tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgagaagg   1500

aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc ggaggcctac   1560

agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg cctttaccag   1620

ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc cctgccccct   1680

cgcactagtg gctccggagc cacgaacttc tctctgttaa agcaagcagg agacgtggaa   1740

gaaaaccccg gtcccatggg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc   1800

ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag   1860

aaagtggact cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag   1920

gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg   1980

actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat   2040

gccactctcg tcttcgatgt ggagcttcta aaactggaag gtggaggcgg ttcaggcggc   2100

ggcggcagcg gcgccatggt cggtgctctt gagagtttga ggggaaatgc agatttggct   2160

tacatcctga gcatggagcc ctgtggccac tgcctcatta tcaacaatgt gaacttctgc   2220

cgtgagtccg ggctccgcac ccgcactggc tccaacatcg actgtgagaa gttgcggcgt   2280

cgcttctcct cgctgcattt catggtggag gtgaagggcg acctgactgc caagaaaatg   2340

gtgctggctt tgctggagct ggcgcggcag gaccacggtg ctctggactg ctgcgtggtg   2400

gtcattctct ctcacggctg tcaggccagc cacctgcagt tcccaggggc tgtctacggc   2460

acagatggat gccctgtgtc ggtcgagaag attgtgaaca tcttcaatgg gaccagctgc   2520

cccagcctgg gagggaagcc caagctcttt ttcatccagg cctgtggtgg ggagcagaaa   2580

gaccatgggt ttgaggtggc ctccacttcc cctgaagacg agtcccctgg cagtaacccc   2640

gagccagatg ccaccccgtt ccaggaaggt ttgaggacct tcgaccagct ggacgccata   2700

tctagtttgc ccacacccag tgacatcttt gtgtcctact ctactttccc aggttttgtt   2760

tcctggaggg accccaagag tggctcctgg tacgttgaga ccctggacga catctttgag   2820

cagtgggctc actctgaaga cctgcagtcc ctcctgctta gggtcgctaa tgctgtttcg   2880

gtgaaaggga tttataaaca gatgcctggt tgctttaatt tcctccggaa aaaacttttc   2940

tttaaaacat cagctagtta a                                             2961


<210>  8
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory signal peptide

<400>  8

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  9
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Secretory signal peptide

<400>  9

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


