                         SEQUENCE LISTING

<110>  Erytech Pharma SA
       Bourgeaux, Vanessa
       Scheer, Alexander
       Senechal, Karine
 
<120>  Synergistic Combinations of Methionine Depletion Agents and 
       Immune Checkpoint Modulators

<130>  ERY2018002

<150>  62/824,249
<151>  2019-03-26

<150>  62/768,036
<151>  2018-11-15

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  398
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide encoding P. putida methionine gamma lyase (MGL) 
       (GenBank  D88554.1)

<400>  1

Met His Gly Ser Asn Lys Leu Pro Gly Phe Ala Thr Arg Ala Ile His 
1               5                   10                  15      


His Gly Tyr Asp Pro Gln Asp His Gly Gly Ala Leu Val Pro Pro Val 
            20                  25                  30          


Tyr Gln Thr Ala Thr Phe Thr Phe Pro Thr Val Glu Tyr Gly Ala Ala 
        35                  40                  45              


Cys Phe Ala Gly Glu Gln Ala Gly His Phe Tyr Ser Arg Ile Ser Asn 
    50                  55                  60                  


Pro Thr Leu Asn Leu Leu Glu Ala Arg Met Ala Ser Leu Glu Gly Gly 
65                  70                  75                  80  


Glu Ala Gly Leu Ala Leu Ala Ser Gly Met Gly Ala Ile Thr Ser Thr 
                85                  90                  95      


Leu Trp Thr Leu Leu Arg Pro Gly Asp Glu Val Leu Leu Gly Asn Thr 
            100                 105                 110         


Leu Tyr Gly Cys Thr Phe Ala Phe Leu His His Gly Ile Gly Glu Phe 
        115                 120                 125             


Gly Val Lys Leu Arg His Val Asp Met Ala Asp Leu Gln Ala Leu Glu 
    130                 135                 140                 


Ala Ala Met Thr Pro Ala Thr Arg Val Ile Tyr Phe Glu Ser Pro Ala 
145                 150                 155                 160 


Asn Pro Asn Met His Met Ala Asp Ile Ala Gly Val Ala Lys Ile Ala 
                165                 170                 175     


Arg Lys His Gly Ala Thr Val Val Val Asp Asn Thr Tyr Cys Thr Pro 
            180                 185                 190         


Tyr Leu Gln Arg Pro Leu Glu Leu Gly Ala Asp Leu Val Val His Ser 
        195                 200                 205             


Ala Thr Lys Tyr Leu Ser Gly His Gly Asp Ile Thr Ala Gly Ile Val 
    210                 215                 220                 


Val Gly Ser Gln Ala Leu Val Asp Arg Ile Arg Leu Gln Gly Leu Lys 
225                 230                 235                 240 


Asp Met Thr Gly Ala Val Leu Ser Pro His Asp Ala Ala Leu Leu Met 
                245                 250                 255     


Arg Gly Ile Lys Thr Leu Asn Leu Arg Met Asp Arg His Cys Ala Asn 
            260                 265                 270         


Ala Gln Val Leu Ala Glu Phe Leu Ala Arg Gln Pro Gln Val Glu Leu 
        275                 280                 285             


Ile His Tyr Pro Gly Leu Ala Ser Phe Pro Gln Tyr Thr Leu Ala Arg 
    290                 295                 300                 


Gln Gln Met Ser Gln Pro Gly Gly Met Ile Ala Phe Glu Leu Lys Gly 
305                 310                 315                 320 


Gly Ile Gly Ala Gly Arg Arg Phe Met Asn Ala Leu Gln Leu Phe Ser 
                325                 330                 335     


Arg Ala Val Ser Leu Gly Asp Ala Glu Ser Leu Ala Gln His Pro Ala 
            340                 345                 350         


Ser Met Thr His Ser Ser Tyr Thr Pro Glu Glu Arg Ala His Tyr Gly 
        355                 360                 365             


Ile Ser Glu Gly Leu Val Arg Leu Ser Val Gly Leu Glu Asp Ile Asp 
    370                 375                 380                 


Asp Leu Leu Ala Asp Val Gln Gln Ala Leu Lys Ala Ser Ala 
385                 390                 395             


<210>  2
<211>  405
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide encoding cysteine gamma lyase (CGL)

<400>  2

Met Gln Glu Lys Asp Ala Ser Ser Gln Gly Phe Leu Pro His Phe Gln 
1               5                   10                  15      


His Phe Ala Thr Gln Ala Ile His Val Gly Gln Asp Pro Glu Gln Trp 
            20                  25                  30          


Thr Ser Arg Ala Val Val Pro Pro Ile Ser Leu Ser Thr Thr Phe Lys 
        35                  40                  45              


Gln Gly Ala Pro Gly Gln His Ser Gly Phe Glu Tyr Ser Arg Ser Gly 
    50                  55                  60                  


Asn Pro Thr Arg Asn Cys Leu Glu Lys Ala Val Ala Ala Leu Asp Gly 
65                  70                  75                  80  


Ala Lys Tyr Cys Leu Ala Phe Ala Ser Gly Leu Ala Ala Thr Val Thr 
                85                  90                  95      


Ile Thr His Leu Leu Lys Ala Gly Asp Gln Ile Ile Cys Met Asp Asp 
            100                 105                 110         


Val Tyr Gly Gly Thr Asn Arg Tyr Phe Arg Gln Val Ala Ser Glu Phe 
        115                 120                 125             


Gly Leu Lys Ile Ser Phe Val Asp Cys Ser Lys Ile Lys Leu Leu Glu 
    130                 135                 140                 


Ala Ala Ile Thr Pro Glu Thr Lys Leu Val Trp Ile Glu Thr Pro Thr 
145                 150                 155                 160 


Asn Pro Thr Gln Lys Val Ile Asp Ile Glu Gly Cys Ala His Ile Val 
                165                 170                 175     


His Lys His Gly Asp Ile Ile Leu Val Val Asp Asn Thr Phe Met Ser 
            180                 185                 190         


Pro Tyr Phe Gln Arg Pro Leu Ala Leu Gly Ala Asp Ile Ser Met Tyr 
        195                 200                 205             


Ser Ala Thr Lys Tyr Met Asn Gly His Ser Asp Val Val Met Gly Leu 
    210                 215                 220                 


Val Ser Val Asn Cys Glu Ser Leu His Asn Arg Leu Arg Phe Leu Gln 
225                 230                 235                 240 


Asn Ser Leu Gly Ala Val Pro Ser Pro Ile Asp Cys Tyr Leu Cys Asn 
                245                 250                 255     


Arg Gly Leu Lys Thr Leu His Val Arg Met Glu Lys His Phe Lys Asn 
            260                 265                 270         


Gly Met Ala Val Ala Gln Phe Leu Glu Ser Asn Pro Trp Val Glu Lys 
        275                 280                 285             


Val Ile Tyr Pro Gly Leu Pro Ser His Pro Gln His Glu Leu Val Lys 
    290                 295                 300                 


Arg Gln Cys Thr Gly Cys Thr Gly Met Val Thr Phe Tyr Ile Lys Gly 
305                 310                 315                 320 


Thr Leu Gln His Ala Glu Ile Phe Leu Lys Asn Leu Lys Leu Phe Thr 
                325                 330                 335     


Leu Ala Glu Ser Leu Gly Gly Phe Glu Ser Leu Ala Glu Leu Pro Ala 
            340                 345                 350         


Ile Met Thr His Ala Ser Val Leu Lys Asn Asp Arg Asp Val Leu Gly 
        355                 360                 365             


Ile Ser Asp Thr Leu Ile Arg Leu Ser Val Gly Leu Glu Asp Glu Glu 
    370                 375                 380                 


Asp Leu Leu Glu Asp Leu Asp Gln Ala Leu Lys Ala Ala His Pro Pro 
385                 390                 395                 400 


Ser Gly Ser His Ser 
                405 


