                         SEQUENCE LISTING

<110>  йũҵѧ
 
<120>  CRISPR-Cas12jøϵͳ

<130>  IEC180202PCT

<150>  CN201811355943.0
<151>  2018-11-15

<160>  108   

<170>  PatentIn version 3.5

<210>  1
<211>  1003
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.3İ

<400>  1

Met Thr Lys Glu Lys Ile Lys Lys Thr Lys Lys Ala Lys Val Glu Lys 
1               5                   10                  15      


Asp Ser Val Thr Arg Ala Gly Ile Leu Arg Ile Leu Leu Asn Pro Asp 
            20                  25                  30          


Gln His Gln Glu Leu Asp Thr Leu Ile Ser Asp His Gln Glu Ala Ala 
        35                  40                  45              


Arg Glu Ile Gln Thr Ala Thr Tyr Lys Leu Ser Gly Leu Lys Leu Tyr 
    50                  55                  60                  


Asp Lys Thr Asn Asn Met Val Val Asp Gly Ser Lys Ala Thr Pro Glu 
65                  70                  75                  80  


Glu Gln Glu Ala Tyr Tyr Lys Ile Ile Asn Trp Glu Gly Gln Pro Ile 
                85                  90                  95      


Ser Ile Ser Asn Pro Met Val Arg Ala Thr Phe Lys Ser Ile Ala Lys 
            100                 105                 110         


Val Lys Glu Asp Ile Arg Arg Lys Gln Glu Glu Tyr Ala Lys Leu Glu 
        115                 120                 125             


Glu Ala Asp Leu Thr Lys Met Ser Thr Gly Asp Val Lys Lys His Lys 
    130                 135                 140                 


Asn Glu Leu Arg Lys Ala Ala Asn Arg Ile Lys His Ser Glu Glu Ile 
145                 150                 155                 160 


Leu Gln Phe Ala Lys Trp Arg Leu Ala Asp Ile Phe Pro Leu Pro Leu 
                165                 170                 175     


Ser His Asn Ser Gln Leu His Leu Lys Asn Asn Tyr His Gln Asn Val 
            180                 185                 190         


Phe Ser Gly Phe His Ala Arg Val Lys Gly Trp Asn Ala Cys Asp Ile 
        195                 200                 205             


Ala Ala Gln Ala Asn Tyr Ala Glu Ile Asp Asn Arg Leu Thr Glu Leu 
    210                 215                 220                 


Ser Ser Glu Leu Ser Gly Asp Tyr Gly Ser Glu Val Ile Thr Asp Leu 
225                 230                 235                 240 


Met Gly Leu Leu Gln Tyr Thr Lys Glu Leu Gly Glu Gly Tyr Thr Asp 
                245                 250                 255     


Thr Ser Tyr Leu Asn Tyr Lys Phe Leu Ser Phe Phe Lys Glu Cys Trp 
            260                 265                 270         


Arg Pro Asn Ala Ile Ala Asn Asn Thr Gly Leu Leu Glu Gly Phe Trp 
        275                 280                 285             


Leu Ala Asn Asn Lys His Thr Asn Lys Lys Asn Gln Val Ala Tyr Ser 
    290                 295                 300                 


Phe Asn Pro Lys Ile Ser Glu Glu Leu Phe Arg Arg Arg Ser Leu Trp 
305                 310                 315                 320 


Glu Ser Asp Lys Cys Leu Leu Ser Asp Pro Arg Phe Glu Lys Tyr Val 
                325                 330                 335     


Glu Leu Phe Asp Lys His Gly Arg Tyr Arg Lys Gly Ala Ser Leu Thr 
            340                 345                 350         


Leu Ile Ser Lys Glu Ser Pro Ile Pro Ile Gly Phe Ser Met Asp Arg 
        355                 360                 365             


Asn Ala Ala Lys Leu Val Arg Ile Asp Asn Asp Thr Ala Asn Arg Gln 
    370                 375                 380                 


Leu Thr Ile Thr Ile Glu Leu Pro Asn Lys Glu Glu Arg Ser Tyr Val 
385                 390                 395                 400 


Ala Ala Tyr Gly Arg Lys His Glu Thr Lys Cys Tyr Tyr Asn Gly Leu 
                405                 410                 415     


Thr Thr Arg Leu Pro Arg Ser Glu Lys Glu Leu Leu Ala Leu Ala Lys 
            420                 425                 430         


Ala Glu Asn Arg Glu Leu Thr Asp Lys Glu Ile His Glu Ala Ser Leu 
        435                 440                 445             


Glu Lys Cys Tyr Ile Phe Glu Tyr Ala Arg Ala Gly Lys Ile Pro Val 
    450                 455                 460                 


Phe Ala Val Val Lys Thr Leu Tyr Phe Arg Arg Asn Pro Ser Asn Gly 
465                 470                 475                 480 


Glu Tyr Tyr Val Ile Leu Pro Thr Asn Ile Phe Val Glu Tyr His Ala 
                485                 490                 495     


Asn Asn Glu Phe Asn Ser Lys Glu Leu Phe Lys Ile Arg Ser Glu Leu 
            500                 505                 510         


Gln Lys Ala Trp Asp Glu Val Arg Thr Pro Lys Arg Asn Val Gln Ser 
        515                 520                 525             


Cys Val Leu Asp Lys Asp Leu Ser Lys Arg Phe Ala Gly Arg Thr Leu 
    530                 535                 540                 


Lys Tyr Ala Gly Ile Asp Leu Gly Tyr Ser Asn Pro Tyr Thr Val Ser 
545                 550                 555                 560 


Tyr Tyr Asn Val Val Gly Thr Glu Glu Gly Ile Gln Ile Lys Glu Thr 
                565                 570                 575     


Gly Asn Glu Ile Val Ser Thr Val Phe Asn Glu Gln Tyr Ile Gln Leu 
            580                 585                 590         


Lys Gly Asn Ile Tyr Gln Leu Ile Asn Ile Ile Arg Ala Ser Arg Arg 
        595                 600                 605             


Tyr Leu Gln Glu Ser Gly Glu Leu Lys Leu Ser Lys Asp Asp Ile Lys 
    610                 615                 620                 


Ser Phe Asp Gln Leu Met Glu Leu Leu Pro Ser Glu Gln Arg Ile Thr 
625                 630                 635                 640 


Ile Asp Gln Phe Ile Lys Asp Ile Lys Lys Ala Lys Gln Glu Gly Lys 
                645                 650                 655     


Leu Ile Arg Asp Ile Lys Gly Lys Leu Pro Val Glu Gly Lys Lys Lys 
            660                 665                 670         


Glu Tyr Trp Val Ile Ser Asn Leu Met Tyr Val Ile Thr Gln Thr Met 
        675                 680                 685             


Asn Gly Ile Arg Gly Asn Arg Asp Ser Asn Asn His Leu Thr Glu Lys 
    690                 695                 700                 


Lys Asn Trp Leu Ser Ala Pro Pro Leu Ile Glu Leu Ile Asp Ala Tyr 
705                 710                 715                 720 


Tyr Asn Leu Lys Lys Thr Phe Asn Asp Ser Gly Asp Gly Ile Lys Met 
                725                 730                 735     


Leu Pro Lys Asp His Val Tyr Ala Glu Gly Glu Lys Gln Arg Cys Thr 
            740                 745                 750         


Leu Arg Glu Glu Asn Phe Cys Lys Gly Ile Leu Glu Trp Arg Asp Asn 
        755                 760                 765             


Val Lys Asp Tyr Phe Ile Lys Lys Leu Phe Ser Gln Ile Ala His Arg 
    770                 775                 780                 


Cys Tyr Glu Leu Gly Ile Gly Ile Val Ala Met Glu Asn Leu Asp Ile 
785                 790                 795                 800 


Met Gly Ser Ser Lys Asn Thr Lys Gln Ser Asn Arg Met Phe Asn Ile 
                805                 810                 815     


Trp Pro Arg Gly Gln Met Lys Lys Ser Ala Glu Asp Ala Phe Ser Tyr 
            820                 825                 830         


Met Gly Ile Leu Ile Gln Tyr Val Asp Glu Asn Gly Thr Ser Arg His 
        835                 840                 845             


Asp Ala Asp Ser Gly Ile Tyr Gly Cys Arg Asp Gly Ala Asn Leu Trp 
    850                 855                 860                 


Leu Pro Asn Lys Lys Leu His Ala Asp Val Asn Ala Ser Arg Met Ile 
865                 870                 875                 880 


Ala Leu Arg Gly Leu Thr His His Thr Asn Leu Tyr Cys Arg Ser Leu 
                885                 890                 895     


Thr Glu Ile Glu Asn Gly Lys Tyr Val Asn Thr Tyr Glu Leu Phe Asp 
            900                 905                 910         


Thr Thr Lys Asn Asp Gln Ser Gly Ala Ala Lys Arg Leu Arg Gly Ala 
        915                 920                 925             


Glu Thr Leu Leu His Gly Tyr Ser Ala Thr Val Tyr Gln Ile His Thr 
    930                 935                 940                 


Thr Asn Thr Gly Ala Gly Val Ala Leu Leu Pro Asp Leu Thr Ala Thr 
945                 950                 955                 960 


Asp Val Ile Lys Asn Lys Lys Ile Thr Ala Thr Lys Glu Asn Thr Ala 
                965                 970                 975     


Lys Tyr Tyr Lys Leu Asp Asn Thr Asn Thr Tyr Tyr Pro Trp Ser Val 
            980                 985                 990         


Cys Glu Lys Leu His Lys Asn Trp  Lys Leu Ser 
        995                 1000             


<210>  2
<211>  874
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.4İ

<400>  2

Met Lys Lys Lys Lys Asn Phe Ser Val Ser Ala Thr Gly Val Phe Ser 
1               5                   10                  15      


Phe Pro Thr Thr Glu Ala Lys Met Asp Phe Phe His Arg Phe Ile Glu 
            20                  25                  30          


Leu Asn Gly Leu Ala Ala Glu Ile Glu Thr His Phe Leu Asn Leu Lys 
        35                  40                  45              


Asn Asp Lys Asn Gly Glu Ser Val Tyr Asn Lys Val Leu Ser Asn Ser 
    50                  55                  60                  


Asn His Ser Arg Pro Phe Ser Thr Pro Leu Leu Gly Thr Met Thr Gly 
65                  70                  75                  80  


Ser Thr Lys Val Thr Asp Lys Asn Ala Leu Tyr Gly Asn Asp Leu Asp 
                85                  90                  95      


His Cys Arg Lys Lys Lys Ile Val Pro Phe Ser Ser Ser Ser Pro Leu 
            100                 105                 110         


Ser Ser Gln Glu Lys Phe Phe Cys Ile Glu Ala Val Phe Arg Arg Ala 
        115                 120                 125             


Lys Ser His Met Glu Cys Lys Lys Leu Phe Gln Asp Glu Thr Asn Arg 
    130                 135                 140                 


Met Asp Ser Gln Ile Asn Gly Ile Leu Asn Glu Leu Pro Tyr Gly Val 
145                 150                 155                 160 


Glu Leu Ser Asn Met Leu Ser Glu Leu Ile Ala Ile Pro Phe Ala Ile 
                165                 170                 175     


Gly Trp Lys Leu Glu Gly Tyr Leu Gly Gln Val Phe Phe Pro Ser Ile 
            180                 185                 190         


Ala Glu Gly Leu Thr Pro Pro Lys Ser Ala Lys Ile Lys Gly Arg Arg 
        195                 200                 205             


Arg Ser Ile Asp Tyr Ser Val Thr Asp Glu Ala Tyr Asp Ile Leu Met 
    210                 215                 220                 


Lys Tyr Ser Asn Leu His Ser Ser Phe Glu Thr Gly Leu Lys Met Ser 
225                 230                 235                 240 


Asn Leu Phe Ser Ala Phe Tyr Lys Lys Ser Asn Arg Lys Asp Glu Ile 
                245                 250                 255     


Gln Phe Thr Pro Ile Ser Met Glu Ser Arg Cys Asp Leu Leu Leu Gly 
            260                 265                 270         


Lys Asn Phe Leu Lys Phe Asp Leu Lys Asn Cys Asp His Arg Ser Gly 
        275                 280                 285             


Ser Leu Met Leu Thr Ile Asn Asp Lys Asn Arg Leu Asn Gly Asp Tyr 
    290                 295                 300                 


Glu Ile Arg Val Gly Ser Asp Lys Lys Asp Ser Tyr Leu Thr Gly Val 
305                 310                 315                 320 


Asn Val Thr Asn Leu Gly Asp Asn Val Phe Asn Leu Asn Tyr Lys Val 
                325                 330                 335     


Asn Gly Lys Arg Glu Tyr Asn Met Leu Leu Lys Glu Pro Ser Ile His 
            340                 345                 350         


Ile Lys Met His Arg Met Arg Asp Asp Gly Asn Tyr Leu Ser Ser Asp 
        355                 360                 365             


Phe Asp Phe Tyr Met Ile Phe Ser Met Ser Ser Glu Lys Asp Glu Glu 
    370                 375                 380                 


Lys Leu Ala Arg Ser Trp Asp Met Arg Ala Ala Met Ser Thr Ala Tyr 
385                 390                 395                 400 


Gly Thr Asp Ile Lys Lys Tyr His Ser Ser Phe Pro Cys Arg Ile Leu 
                405                 410                 415     


Ala Cys Asp Leu Gly Val Lys His Pro Tyr Ser Ala Ala Val Met Asp 
            420                 425                 430         


Ile Gly Gln Leu Asn Glu Asn Gly Met Pro Val Ser Val Asp Lys Val 
        435                 440                 445             


His Cys Met His Ser Glu Gly Val Ser Glu Ile Gly Gln Gly Tyr Asn 
    450                 455                 460                 


His Leu Ile Gln Lys Ile Leu Ala Leu Asn Tyr Ile Leu Ala Tyr Cys 
465                 470                 475                 480 


Arg Glu Phe Val Ser Gly Thr Val Asp Asp Phe Asp Lys Ile Asp Tyr 
                485                 490                 495     


Lys Leu Ser Gln Leu Ser Tyr Lys Gln Glu Asp Leu Leu Ile Asn Leu 
            500                 505                 510         


Gln Glu Met Lys Asp His Phe Gly Asn Asp Met Gln Ala Trp Lys Lys 
        515                 520                 525             


Ser Arg Thr Trp Val Val Ser Thr Leu Phe Phe Glu Leu Arg Gln Glu 
    530                 535                 540                 


Phe Asn Gln Leu Arg Asn Gln Arg Pro Gly Lys Lys Thr Val Ser Leu 
545                 550                 555                 560 


Ala Asp Glu Phe Gln Tyr Ile Asp Met Arg Arg Lys Phe Ile Ser Leu 
                565                 570                 575     


Ser Arg Ser Tyr Thr Asn Val Gly Arg Gln Ser Ser Lys His Arg His 
            580                 585                 590         


Asp Ser Tyr Gln Thr His Tyr Asp Val Ile Asn Arg Cys Lys Lys Asn 
        595                 600                 605             


Leu Leu Arg Asn Ile Cys Arg Arg Met Ile Asp Met Ala Val Gln Asn 
    610                 615                 620                 


Lys Cys Asp Ile Ile Val Val Glu Asp Leu Ser Phe Gln Leu Ser Ser 
625                 630                 635                 640 


His Asn Ser Arg Arg Asp Asn Val Phe Asn Ala Leu Trp Ser Cys Lys 
                645                 650                 655     


Ser Ile Lys Asn Met Leu Gly Ile Met Ala Glu Gln His Asn Ile Ile 
            660                 665                 670         


Ile Ser Glu Val Asp Pro Asn His Thr Ser Lys Ile Asp Cys Glu Thr 
        675                 680                 685             


Gly Asn Phe Gly Tyr Arg Tyr Ser Ser Asp Phe Tyr Ser Val Ile Asp 
    690                 695                 700                 


Gly Gln Leu Val Arg Arg His Ala Asp Glu Asn Ala Ala Ile Asn Ile 
705                 710                 715                 720 


Gly Asn Arg Trp Ala Ser Arg His Thr Asp Leu Lys Ser Phe Asn Cys 
                725                 730                 735     


Arg Gln Ile Ser Ile Asp Gly Arg Lys Val Ala Phe Pro Tyr Ala Lys 
            740                 745                 750         


Gly Lys Arg Lys Ser Ala Leu Phe Gly Tyr Leu Phe Gly Asn Cys Lys 
        755                 760                 765             


Thr Val Phe Val Ser Asp Asp Gly Asp Ser Tyr Thr Pro Ile Pro Tyr 
    770                 775                 780                 


Ser Lys Phe Arg Lys Ser Ile Ser Lys Asp Asp His Asp Val Val Asn 
785                 790                 795                 800 


Tyr Leu His Asp Leu Thr Met Asn Lys Asn Val Ile Arg Val Glu Tyr 
                805                 810                 815     


Asn Lys Ser Ile Lys Ser Ala Ser Val Glu Leu Tyr Leu Asn Asp Asp 
            820                 825                 830         


Arg Val Ile Ser Arg Ser Leu Arg Asp Lys Glu Val Asp Ala Ile Glu 
        835                 840                 845             


Lys Leu Val Ser Arg Gly Ser Leu Ile Asn Glu Ser Gly Pro Ser Leu 
    850                 855                 860                 


Glu His Asp Glu Val Lys Ser Val Thr His 
865                 870                 


<210>  3
<211>  870
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.5İ

<400>  3

Met Lys Val His Glu Ile Pro Arg Ser Gln Leu Leu Lys Ile Lys Gln 
1               5                   10                  15      


Tyr Glu Gly Ser Phe Val Glu Trp Tyr Arg Asp Leu Gln Glu Asp Arg 
            20                  25                  30          


Lys Lys Phe Ala Ser Leu Leu Phe Arg Trp Ala Ala Phe Gly Tyr Ala 
        35                  40                  45              


Ala Arg Glu Asp Asp Gly Ala Thr Tyr Ile Ser Pro Ser Gln Ala Leu 
    50                  55                  60                  


Leu Glu Arg Arg Leu Leu Leu Gly Asp Ala Glu Asp Val Ala Ile Lys 
65                  70                  75                  80  


Phe Leu Asp Val Leu Phe Lys Gly Gly Ala Pro Ser Ser Ser Cys Tyr 
                85                  90                  95      


Ser Leu Phe Tyr Glu Asp Phe Ala Leu Arg Asp Lys Ala Lys Tyr Ser 
            100                 105                 110         


Gly Ala Lys Arg Glu Phe Ile Glu Gly Leu Ala Thr Met Pro Leu Asp 
        115                 120                 125             


Lys Ile Ile Glu Arg Ile Arg Gln Asp Glu Gln Leu Ser Lys Ile Pro 
    130                 135                 140                 


Ala Glu Glu Trp Leu Ile Leu Gly Ala Glu Tyr Ser Pro Glu Glu Ile 
145                 150                 155                 160 


Trp Glu Gln Val Ala Pro Arg Ile Val Asn Val Asp Arg Ser Leu Gly 
                165                 170                 175     


Lys Gln Leu Arg Glu Arg Leu Gly Ile Lys Cys Arg Arg Pro His Asp 
            180                 185                 190         


Ala Gly Tyr Cys Lys Ile Leu Met Glu Val Val Ala Arg Gln Leu Arg 
        195                 200                 205             


Ser His Asn Glu Thr Tyr His Glu Tyr Leu Asn Gln Thr His Glu Met 
    210                 215                 220                 


Lys Thr Lys Val Ala Asn Asn Leu Thr Asn Glu Phe Asp Leu Val Cys 
225                 230                 235                 240 


Glu Phe Ala Glu Val Leu Glu Glu Lys Asn Tyr Gly Leu Gly Trp Tyr 
                245                 250                 255     


Val Leu Trp Gln Gly Val Lys Gln Ala Leu Lys Glu Gln Lys Lys Pro 
            260                 265                 270         


Thr Lys Ile Gln Ile Ala Val Asp Gln Leu Arg Gln Pro Lys Phe Ala 
        275                 280                 285             


Gly Leu Leu Thr Ala Lys Trp Arg Ala Leu Lys Gly Ala Tyr Asp Thr 
    290                 295                 300                 


Trp Lys Leu Lys Lys Arg Leu Glu Lys Arg Lys Ala Phe Pro Tyr Met 
305                 310                 315                 320 


Pro Asn Trp Asp Asn Asp Tyr Gln Ile Pro Val Gly Leu Thr Gly Leu 
                325                 330                 335     


Gly Val Phe Thr Leu Glu Val Lys Arg Thr Glu Val Val Val Asp Leu 
            340                 345                 350         


Lys Glu His Gly Lys Leu Phe Cys Ser His Ser His Tyr Phe Gly Asp 
        355                 360                 365             


Leu Thr Ala Glu Lys His Pro Ser Arg Tyr His Leu Lys Phe Arg His 
    370                 375                 380                 


Lys Leu Lys Leu Arg Lys Arg Asp Ser Arg Val Glu Pro Thr Ile Gly 
385                 390                 395                 400 


Pro Trp Ile Glu Ala Ala Leu Arg Glu Ile Thr Ile Gln Lys Lys Pro 
                405                 410                 415     


Asn Gly Val Phe Tyr Leu Gly Leu Pro Tyr Ala Leu Ser His Gly Ile 
            420                 425                 430         


Asp Asn Phe Gln Ile Ala Lys Arg Phe Phe Ser Ala Ala Lys Pro Asp 
        435                 440                 445             


Lys Glu Val Ile Asn Gly Leu Pro Ser Glu Met Val Val Gly Ala Ala 
    450                 455                 460                 


Asp Leu Asn Leu Ser Asn Ile Val Ala Pro Val Lys Ala Arg Ile Gly 
465                 470                 475                 480 


Lys Gly Leu Glu Gly Pro Leu His Ala Leu Asp Tyr Gly Tyr Gly Glu 
                485                 490                 495     


Leu Ile Asp Gly Pro Lys Ile Leu Thr Pro Asp Gly Pro Arg Cys Gly 
            500                 505                 510         


Glu Leu Ile Ser Leu Lys Arg Asp Ile Val Glu Ile Lys Ser Ala Ile 
        515                 520                 525             


Lys Glu Phe Lys Ala Cys Gln Arg Glu Gly Leu Thr Met Ser Glu Glu 
    530                 535                 540                 


Thr Thr Thr Trp Leu Ser Glu Val Glu Ser Pro Ser Asp Ser Pro Arg 
545                 550                 555                 560 


Cys Met Ile Gln Ser Arg Ile Ala Asp Thr Ser Arg Arg Leu Asn Ser 
                565                 570                 575     


Phe Lys Tyr Gln Met Asn Lys Glu Gly Tyr Gln Asp Leu Ala Glu Ala 
            580                 585                 590         


Leu Arg Leu Leu Asp Ala Met Asp Ser Tyr Asn Ser Leu Leu Glu Ser 
        595                 600                 605             


Tyr Gln Arg Met His Leu Ser Pro Gly Glu Gln Ser Pro Lys Glu Ala 
    610                 615                 620                 


Lys Phe Asp Thr Lys Arg Ala Ser Phe Arg Asp Leu Leu Arg Arg Arg 
625                 630                 635                 640 


Val Ala His Thr Ile Val Glu Tyr Phe Asp Asp Cys Asp Ile Val Phe 
                645                 650                 655     


Phe Glu Asp Leu Asp Gly Pro Ser Asp Ser Asp Ser Arg Asn Asn Ala 
            660                 665                 670         


Leu Val Lys Leu Leu Ser Pro Arg Thr Leu Leu Leu Tyr Ile Arg Gln 
        675                 680                 685             


Ala Leu Glu Lys Arg Gly Ile Gly Met Val Glu Val Ala Lys Asp Gly 
    690                 695                 700                 


Thr Ser Gln Asn Asn Pro Ile Ser Gly His Val Gly Trp Arg Asn Lys 
705                 710                 715                 720 


Gln Asn Lys Ser Glu Ile Tyr Phe Tyr Glu Asp Lys Glu Leu Leu Val 
                725                 730                 735     


Met Asp Ala Asp Glu Val Gly Ala Met Asn Ile Leu Cys Arg Gly Leu 
            740                 745                 750         


Asn His Ser Val Cys Pro Tyr Ser Phe Val Thr Lys Ala Pro Glu Lys 
        755                 760                 765             


Lys Asn Asp Glu Lys Lys Glu Gly Asp Tyr Gly Lys Arg Val Lys Arg 
    770                 775                 780                 


Phe Leu Lys Asp Arg Tyr Gly Ser Ser Asn Val Arg Phe Leu Val Ala 
785                 790                 795                 800 


Ser Met Gly Phe Val Thr Val Thr Thr Lys Arg Pro Lys Asp Ala Leu 
                805                 810                 815     


Val Gly Lys Arg Leu Tyr Tyr His Gly Gly Glu Leu Val Thr His Asp 
            820                 825                 830         


Leu His Asn Arg Met Lys Asp Glu Ile Lys Tyr Leu Val Glu Lys Glu 
        835                 840                 845             


Val Leu Ala Arg Arg Val Ser Leu Ser Asp Ser Thr Ile Lys Ser Tyr 
    850                 855                 860                 


Lys Ser Phe Ala His Val 
865                 870 


<210>  4
<211>  964
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.6İ

<400>  4

Met Ser Ala Asn Arg Val Ser Ala Asn Ser Gln Phe Glu Leu Gly Tyr 
1               5                   10                  15      


Pro Met Ser Leu Ser Leu Arg Gly Lys Val Phe Asn Ser Arg Glu Met 
            20                  25                  30          


Met Lys Glu Ile Leu Pro Val Met Asn Asn Ile Val His Tyr Gln Asn 
        35                  40                  45              


Asn Leu Leu Lys Leu Met Leu Ile Leu Arg Gly Glu Lys Tyr Thr Leu 
    50                  55                  60                  


Asp Gly Gln Phe Phe Ser Gln Lys Asp Val Asp Arg Gln Phe Gly Asp 
65                  70                  75                  80  


Leu Cys Lys Glu His Asn Ile Lys Gly Ser Ile Cys Ser Leu Lys Glu 
                85                  90                  95      


Lys Ser Arg Lys Leu Tyr Glu Val Phe Ser Cys Tyr Ile Asp Lys Lys 
            100                 105                 110         


Gly Asn Leu Lys Thr Asn Ser Lys Ala Arg Ser Phe Ala Gly Val Leu 
        115                 120                 125             


Leu Asn Pro Lys Asp Val Lys Leu Pro Pro Gln Ile Asp Ser Ile Ser 
    130                 135                 140                 


Ser Phe Val Val Glu Leu Arg Ala Lys Gly Val Leu Pro Ile Lys His 
145                 150                 155                 160 


Glu Gly Asn Tyr Leu Ser Gly His Pro Ser Leu Lys Tyr Ser Val Ala 
                165                 170                 175     


Gln Asn Val Leu Val Lys Leu Thr Ser Met Glu Lys Leu Gln Lys Ile 
            180                 185                 190         


Tyr Ser Asp Glu Lys Ala Gly Trp Glu Asn Ile Val Ser Glu Val Arg 
        195                 200                 205             


Ser Asp Leu Pro Lys Ile Glu Arg Tyr Glu Arg Met Leu Leu Ser Ile 
    210                 215                 220                 


Lys Ala Val Lys Glu Met Glu Lys Phe Gly Ile Asn Asn Tyr Arg His 
225                 230                 235                 240 


Leu Leu Asn Asn Trp Arg Asp Glu Val Asp Lys Asp Ser Gly Lys Val 
                245                 250                 255     


Leu Lys Gln Gly Met Arg Thr Tyr Phe Val Asn Met Leu Glu Ser Lys 
            260                 265                 270         


Lys Asp Tyr Arg Phe Glu Glu Ser Asp Arg Tyr Leu Phe Gly Tyr Ala 
        275                 280                 285             


Pro Glu Val Met Asn Leu Val Tyr His Asp Phe Arg Asp Leu Trp Gln 
    290                 295                 300                 


Gly Glu Asp Ile Ile Gly Ser Gln Ser Pro Glu Lys Lys Asp Arg Asp 
305                 310                 315                 320 


Tyr Val Asp Val Ile Phe Asn Tyr Phe Asn Trp Arg Lys Glu Ser Ile 
                325                 330                 335     


Asn Ile Ser Ser Phe Asp Ser Tyr Gly Lys Thr Ala Gln Ile Lys Leu 
            340                 345                 350         


Gly Asp Asn Tyr Val Pro Phe Ser Asn Phe Gln Tyr Asp Lys Ile Leu 
        355                 360                 365             


Asp Ala Trp Thr Leu Glu Ile Ala Asn Val Ser Gly Glu Gly Asp Asn 
    370                 375                 380                 


His Lys Leu Val Ile Ala Arg Ser Pro Gln Phe Asp Ser His Ser Ser 
385                 390                 395                 400 


Val Lys Asp Ile Val Met Lys Asn Leu Lys Gly Lys Glu Ala Ser Lys 
                405                 410                 415     


Thr Thr Leu Glu Phe Arg Tyr Ser Gly Asp Ser Lys Lys Ser Thr Trp 
            420                 425                 430         


Tyr Arg Gly Thr Leu Lys Glu Pro Thr Leu Arg Tyr Ser Ser Ser Lys 
        435                 440                 445             


Asn Cys Leu Tyr Val Asp Phe Ala Leu Ser Asn His Ile Val Glu Gly 
    450                 455                 460                 


Leu Ile Ser Asp Asn Leu Gly Ile Ser Asp Lys Met Tyr Lys Phe Arg 
465                 470                 475                 480 


Gly Glu Phe Met Lys Ala Ser Pro Ser Ser Gly Lys Gln Ser Asn Ser 
                485                 490                 495     


Ile Asn Leu Pro Ile Lys Lys Leu Arg Ala Met Gly Val Asp Phe Asn 
            500                 505                 510         


Leu Arg Arg Pro Phe Gln Ala Ser Ile Tyr Asp Val Glu Asn Lys Asn 
        515                 520                 525             


Gly Asn Leu Glu Phe Ser Phe Val Lys His Val Gln Ser Phe Ser Asn 
    530                 535                 540                 


Glu Asn Asp Glu Glu Arg Ala Lys Glu Leu Leu Asn Ile Glu Arg Asn 
545                 550                 555                 560 


Ile Leu Ala Leu Lys Ile Leu Ile Trp Gln Thr Val Gly Tyr Val Thr 
                565                 570                 575     


Gly Lys Asn Asp Thr Ile Asp Gly Val Val Thr Arg Lys Asn Asn Ala 
            580                 585                 590         


Val Asp Ile Glu Lys Thr Leu Gly Ile Asn Met Lys Glu Tyr Met Ala 
        595                 600                 605             


Tyr Leu Asn Gln Phe Arg Ser Tyr Glu Asp Lys Asn Lys Ala Phe Met 
    610                 615                 620                 


Asp Leu Arg Lys Arg Glu Tyr Ala Trp Ile Val Pro Pro Leu Ile Phe 
625                 630                 635                 640 


Gln Cys Arg Ser Arg Leu Ile Ser Phe Arg Ser Glu Tyr Phe Asn Thr 
                645                 650                 655     


Pro Lys Asp Glu Lys Ser His Tyr Cys Gln His Arg Asn Phe Val Asp 
            660                 665                 670         


Tyr Ser Thr Phe Leu Lys Lys Asn Val Val Lys Lys Met Met Glu Leu 
        675                 680                 685             


Arg Arg Ser Tyr Ser Thr Phe Gly Met Ser Ser Glu Gln Ser Ile Trp 
    690                 695                 700                 


Val Thr Asn Asn Asp His Ala Lys Asp Gly Ser Lys Lys Asn Gly Asn 
705                 710                 715                 720 


Met Phe Asp Asp Asp Leu His Gln Trp Tyr Asn Gly Leu Val Arg Lys 
                725                 730                 735     


Cys Ser Ser Leu Ala Ser Ser Ile Ile Asn Val Ala Arg Asp Asn Gly 
            740                 745                 750         


Ala Ile Leu Val Phe Ile Glu Asp Leu Asp Cys His Pro Ser Ala Phe 
        755                 760                 765             


Asp Ser Glu Glu Asp Asn Ser Leu Lys Ser Ile Trp Gly Trp Gly Ser 
    770                 775                 780                 


Ile Lys Ala Ser Leu Ala His Gln Ala Arg Lys His Asn Ile Ala Val 
785                 790                 795                 800 


Val Ala Asn Asp Pro His Leu Thr Ser Leu Val Ser Ser Thr Thr Gly 
                805                 810                 815     


Glu Leu Gly Ile Ala Lys Gly Arg Asp Val Leu Phe Phe Asp Ser Lys 
            820                 825                 830         


Gly Lys Leu Thr Ser Lys Val Asn Arg Asp Glu Asn Ala Ala Gln Asn 
        835                 840                 845             


Ile Ala Ile Arg Gly Phe Val Arg His Ser Asp Leu Arg Glu Phe Val 
    850                 855                 860                 


Ala Glu Lys Ile Glu Glu Asn Arg Tyr Arg Val Val Val Asn Lys Thr 
865                 870                 875                 880 


His Lys Arg Lys Ala Gly Ala Ile Tyr Arg His Ile Gly Ser Thr Glu 
                885                 890                 895     


Cys Ile Met Ser Lys Gln Ala Asp Gly Ser Leu Lys Ile Asp Lys Thr 
            900                 905                 910         


Glu Leu Thr Pro Leu Glu Ile Lys Met Glu Lys Lys Asn Asp Lys Lys 
        915                 920                 925             


Met Tyr Val Ile Leu His Gly Lys Thr Trp Arg Leu Arg His Glu Leu 
    930                 935                 940                 


Asn Glu Lys Leu Glu Lys Asp Leu Asp Asn His Leu Lys Ser Lys Ser 
945                 950                 955                 960 


Ser Val Ile Ser 
                


<210>  5
<211>  962
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.7İ

<400>  5

Met Ser Ser Ala Asn Asp Gln Leu Gly Leu Gly Tyr Pro Leu Thr Leu 
1               5                   10                  15      


Thr Leu Arg Gly Lys Val Tyr Asn His Asp Thr Ala Met Glu Ala Phe 
            20                  25                  30          


Ala Pro Val Met Lys Gly Met Val Pro Tyr Ala Asn Asn Leu Met Arg 
        35                  40                  45              


Ile Leu Leu Thr Leu Arg Leu Glu Lys Tyr Thr Leu Asp Gly Ile His 
    50                  55                  60                  


His Thr Lys Glu Glu Val Glu Lys Asp Leu Arg Gly Leu Met Lys Glu 
65                  70                  75                  80  


Tyr Gly Ile Asn Leu Ser Phe Ala Lys Phe Ser Glu Met Ala Gly Glu 
                85                  90                  95      


Val Tyr Arg Val Phe Val Cys Tyr Val Asp Ala Lys Gly Lys Leu Lys 
            100                 105                 110         


Val Asn Gly Lys Ala Arg Gly Phe Ala Asn Val Phe Phe Ser Glu Asp 
        115                 120                 125             


Asp Ala Thr Ile Pro Glu Asn Cys Pro Ser Met Glu Leu Leu Arg Lys 
    130                 135                 140                 


Lys Gly Met Phe Pro Ile Leu Val Asp Gly Lys Pro Ile Ser Ser Ile 
145                 150                 155                 160 


Ser Arg Glu Lys Thr Pro Leu Lys Tyr Ser Val Ala Gln Asp Val Leu 
                165                 170                 175     


Thr Lys Leu Thr Ser Met Glu Glu Ile Ser Lys Glu Tyr Glu Lys Ala 
            180                 185                 190         


Lys Thr Asp Trp Glu Asn Glu Cys Gln Lys Val Ile Ser Gln Leu Pro 
        195                 200                 205             


Leu Ile Gly Arg Tyr Glu Ala Leu Leu Thr Thr Ile Pro Leu Ile Pro 
    210                 215                 220                 


Glu Met Arg Gly Phe Asp Gly Asp Asn Tyr Arg Lys Met Leu Asn Arg 
225                 230                 235                 240 


Trp Arg Asp Tyr Val Asn Glu Asp Gly Glu Leu Val Arg Gly Gly Met 
                245                 250                 255     


Lys Thr Tyr Phe Leu Asp Leu Leu Ser Lys Asp Thr Ser His Lys Phe 
            260                 265                 270         


Asn Glu Glu Glu Arg Tyr Leu Phe Gly Tyr Cys Pro Glu Phe Met Asn 
        275                 280                 285             


Leu Ile Tyr His Asp Phe Arg Asp Leu Trp Ser Lys Glu Asp Ile Ile 
    290                 295                 300                 


Gly Ser Gln Arg Lys Gly Lys Gly Leu Lys Gly Lys Asp Tyr Val Asp 
305                 310                 315                 320 


Val Ile Phe Asn Cys Phe His Trp Arg Arg Glu Ser Ile Asn Ile Ser 
                325                 330                 335     


Ser Phe Gly Asn Asn Asp Lys Val Met Asn Ile His Leu Gly Asp Asn 
            340                 345                 350         


Phe Val Pro Phe Glu Leu Lys Ser Gln Asn Gly Ile Trp Glu Val His 
        355                 360                 365             


Val Gln Asn Leu His Gly Gln Asn Asp Pro His Arg Val Ile Val Cys 
    370                 375                 380                 


Arg Cys Pro Gln Phe Asn Glu Asp Ser Ser Met Lys Met Val His Pro 
385                 390                 395                 400 


Leu Ala Lys Asn Gly Glu Glu Ser Asp Lys Glu Asn Ile Glu Phe Arg 
                405                 410                 415     


Tyr Ser Gly Asp Ser Lys Arg Glu Thr Trp Tyr Thr Gly Leu Leu Lys 
            420                 425                 430         


Glu Pro Thr Leu Arg Tyr Asp Val Glu Arg Lys Ser Leu Tyr Val Asp 
        435                 440                 445             


Phe Ile Leu Ser Asn His Arg Val Glu Gly Val Val Thr Asn Glu Tyr 
    450                 455                 460                 


Leu Lys Asp Pro Arg Asp Leu Phe Gly Val Arg Gly Tyr Phe Leu Ser 
465                 470                 475                 480 


Ser Ser Val Ser Asn Pro Arg Gln Lys Asp Lys Thr Ser Leu Pro Asp 
                485                 490                 495     


Gly Lys Phe Asn Val Met Gly Val Asp Leu Gly Leu Lys Cys Pro Tyr 
            500                 505                 510         


Glu Cys Ala Ile Tyr Gly Ile Thr Val Lys Asn Gly Lys Met Gln His 
        515                 520                 525             


Lys Trp Ser His Asn Val Ser Ala Glu Asp Asn Asn Asn Val Ser Glu 
    530                 535                 540                 


Arg Leu Ala Asn Leu Lys Lys Ile Asp Glu Lys Ile Leu Ala Thr Gln 
545                 550                 555                 560 


Val Leu Ile Ser Leu Thr Lys Met Cys Val Val Lys Asp Glu Glu Ile 
                565                 570                 575     


Pro Asp Ser Tyr Thr Leu Arg Glu His Arg Val Asp Ile Ala Lys Ser 
            580                 585                 590         


Leu Asp Leu Asp Met Asp Lys Tyr Arg Arg Tyr Val Glu Lys Cys Lys 
        595                 600                 605             


Lys Asn Pro Asp Lys Ile Gln Ala Leu Lys Asp Ile Arg Lys Ser Glu 
    610                 615                 620                 


Asn Asn Trp Ile Val Ala Glu Lys Ile Asn Glu Ile Arg Ser Leu Ile 
625                 630                 635                 640 


Ser Glu Ile Arg Ser Glu Tyr Tyr Ala Ser Lys Asp Lys Arg Asn Tyr 
                645                 650                 655     


Cys Arg Asn Leu Asn Gly Val Asp Leu Ser Val Phe Leu Lys Lys Lys 
            660                 665                 670         


Val Val Lys Asn Trp Ile Ser Leu Leu Arg Ser Phe Ser Thr Phe Gly 
        675                 680                 685             


Met Thr Pro Gln Glu Ser Ala Tyr Ile Arg Lys Asp Phe Ala Lys Asn 
    690                 695                 700                 


Leu Ser Lys Trp Tyr Lys Gly Leu Val Arg Lys Cys Gly Ser Ile Ala 
705                 710                 715                 720 


Ala His Ile Val Asn Ile Ala Arg Asp Asn Lys Val Met Val Ile Phe 
                725                 730                 735     


Ile Glu Asp Leu Asp Ala Arg Thr Ser Ala Phe Asp Ser Lys Glu Asp 
            740                 745                 750         


Asn Glu Leu Lys Ile Leu Trp Gly Trp Gly Glu Ile Lys Lys Trp Ile 
        755                 760                 765             


Gly His Gln Ala Arg Lys His Asn Ile Ala Val Val Ala Val Asp Pro 
    770                 775                 780                 


His Leu Thr Ser Leu Val Asn His Glu Ser Gly Leu Leu Gly Ile Ala 
785                 790                 795                 800 


Gly Ser Gly Asn Asp Arg Asn Ile Tyr Thr Phe Gln Lys Asn Lys Lys 
                805                 810                 815     


Tyr Val Val Ile Asn Arg Asp Asn Asn Ala Ala His Asn Ile Ala Leu 
            820                 825                 830         


Arg Gly Leu Ser Lys His Thr Asp Ile Arg Glu Phe Tyr Val Glu Gln 
        835                 840                 845             


Ile Asp Val Asp His Tyr Arg Leu Met Tyr Gly Pro Glu Ala Glu Asn 
    850                 855                 860                 


Gly Lys Arg Arg Ser Gly Ala Ile Tyr Lys His Ile Gly Ser Thr Glu 
865                 870                 875                 880 


Cys Val Phe Ser Lys Gln Lys Asn Gly Thr Leu Lys Val Glu Lys Thr 
                885                 890                 895     


Ser Leu Thr Lys Asp Glu Lys Glu Met Pro Lys Ile Asn Gly Lys Gly 
            900                 905                 910         


Val Tyr Ala Ile Leu His Gly Asn Glu Trp Arg Leu Arg His Glu Leu 
        915                 920                 925             


Asn Glu Glu Leu Gly Ala Lys Leu Asp Gly Ile Ser Val Lys Arg Val 
    930                 935                 940                 


Val Ser Glu Pro Asn Lys Val Lys Thr Ser Leu Val Lys Gly Ser Val 
945                 950                 955                 960 


Arg Ala 
        


<210>  6
<211>  907
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.8İ

<400>  6

Met Lys Lys Gln Thr Ile Val Lys Lys Asp Ser Lys Ala Glu Thr Lys 
1               5                   10                  15      


Glu Asn Lys Met Tyr Pro Asp Lys Asp Thr Asp Phe Pro Val Asn Ser 
            20                  25                  30          


Gln Phe Ser Arg Ser Ile Ser Ile Arg Ala Asn Val Asp Pro Lys Asp 
        35                  40                  45              


Leu Leu Val Leu Lys Arg Thr Phe Glu Glu Thr Thr Lys Ile Ser Asp 
    50                  55                  60                  


Glu Leu Leu Ser Thr Leu Leu Met Leu Arg Gly Lys Asp Tyr Cys Leu 
65                  70                  75                  80  


Asp Asn Val Val Cys Lys Gly Glu Glu Val Leu Glu Asn Leu Tyr Lys 
                85                  90                  95      


Lys Leu Ser Lys Asn Ala Thr Val Asn Arg Asp Lys Phe Ile Ser Thr 
            100                 105                 110         


Ala Lys Ala Phe Tyr Glu Tyr Phe His Gly Cys Ser Tyr His Lys Gly 
        115                 120                 125             


Phe Lys Ser Phe Phe Phe Ser Ser Lys Glu Ile Asp Ser Ile Gln Ser 
    130                 135                 140                 


Glu Lys Phe Gly Tyr Leu Arg Glu Ile Gly Leu Phe Pro Ile Lys Ile 
145                 150                 155                 160 


Asp Ala Gln Ile Ser Asn Asp Leu Gln Tyr Ser Ile Val Ala Ser Asn 
                165                 170                 175     


His Ala Lys Ile Lys Gly Phe Glu Lys Ile Asp Lys Glu Tyr Gln Ala 
            180                 185                 190         


Asn Lys Glu Lys Trp Asn Lys Thr Ile Gly Glu Ser Thr Leu Lys His 
        195                 200                 205             


Leu Asn Arg Tyr Gly Glu Met Leu Lys Gly Leu Ser Asp Leu Gly Thr 
    210                 215                 220                 


Met Gly Asn Phe Asn Gly Lys Lys Tyr Asp Arg Phe Met Gly His Trp 
225                 230                 235                 240 


Arg Asn Glu Gln Lys Ile Pro Asp His Ile Ser Met Leu Asp Phe Phe 
                245                 250                 255     


Arg Lys Ile Tyr Gln Glu Lys Gly Lys Ser His Arg Phe Thr Ala Ile 
            260                 265                 270         


Asp Asn Phe Thr Tyr Gly Tyr Glu Ser Glu Phe Met Asn His Ile Tyr 
        275                 280                 285             


Leu Asn Phe Ser Asp Leu Trp Leu Lys Glu Asp Val Ile Gly Asp Glu 
    290                 295                 300                 


Glu Tyr Val Ser Leu Ile Arg Gly Ala Tyr His Trp Gln Lys Asp Val 
305                 310                 315                 320 


Val Gly Ile Ala Ser Phe Ser Gly Tyr Asn Lys Tyr Glu Lys Leu Phe 
                325                 330                 335     


Met Gly Asp Asn Lys Ile Asn Tyr Ala Leu Asp Phe Ser Asn Lys Asp 
            340                 345                 350         


Gln Trp Leu Met Lys Phe Asn Asn Val Ile Ser Lys Glu Pro Glu Thr 
        355                 360                 365             


Ile Thr Leu Arg Leu Cys Lys Asn Gly Tyr Phe Asn Asn Leu Ser Val 
    370                 375                 380                 


Leu Glu Lys Asn Asp Glu Asn Gly Arg Tyr Lys Ile Arg Phe Ser Thr 
385                 390                 395                 400 


Glu Lys Gln Gly Lys Tyr Phe Tyr Glu Ala Phe Ile Arg Glu Pro Phe 
                405                 410                 415     


Leu Arg Tyr Asn Lys Asp Asn Asp Lys Ile Tyr Val His Phe Cys Leu 
            420                 425                 430         


Ser Glu Glu Ile Lys Glu Asn Cys Pro Asn His Leu Asp Thr Arg Ser 
        435                 440                 445             


Asp Lys Tyr Leu Phe Lys Ser Ala Leu Leu Thr Asn Ser Arg Gln Lys 
    450                 455                 460                 


Leu Gly Lys Leu His Tyr Arg Asp Phe His Ile Val Gly Val Asp Leu 
465                 470                 475                 480 


Gly Ile Asn Pro Val Ala Lys Ile Thr Val Cys Lys Val His Val Asp 
                485                 490                 495     


Lys Asn Glu Asn Leu Lys Ile Thr Lys Ile Ile Thr Glu Glu Thr Arg 
            500                 505                 510         


Lys Asn Ile Asp Thr Asn Tyr Leu Asp Gln Leu Asn Leu Leu Tyr Lys 
        515                 520                 525             


Lys Ile Val Ser Leu Lys Arg Leu Ile Arg Ala Thr Val Ala Phe Lys 
    530                 535                 540                 


Lys Asp Gly Glu Glu Ile Pro Lys Met Phe Lys Met Gly Lys Lys Ser 
545                 550                 555                 560 


Pro Tyr Phe Leu Asn Trp Thr Glu Val Leu Asn Val Asn Tyr Asp Asp 
                565                 570                 575     


Tyr Ile Lys Glu Ile Ser Thr Phe Ser Val Asp Arg Leu Ser Gly Leu 
            580                 585                 590         


Thr Leu Pro Met Gln Trp Ala Arg Ser Gln Asn Lys Trp Val Val Lys 
        595                 600                 605             


Asp Leu Thr Lys Met Val Arg Lys Gly Ile Ser Asp Leu Ile Tyr Ala 
    610                 615                 620                 


Arg Tyr Phe Asn Cys Ser Asp Lys Thr Gln Tyr Val Thr Glu Asn Asn 
625                 630                 635                 640 


Ala Val Asp Ile Thr Thr Phe Lys Lys His Asp Ile Ile Ser Glu Ile 
                645                 650                 655     


Ile Gly Leu Gln Lys Met Phe Ser Gly Gly Gly Lys Asp Val Ala Lys 
            660                 665                 670         


Lys Asp Tyr Leu Tyr Leu Arg Gly Leu Arg Lys His Ile Gly Asn Tyr 
        675                 680                 685             


Thr Ala Ser Ala Ile Val Ser Ile Ala Gln Lys Tyr Asn Ala Val Phe 
    690                 695                 700                 


Ile Phe Ile Glu Asp Leu Asp Leu Lys Ile Ser Gly Met Asn Gly Lys 
705                 710                 715                 720 


Lys Glu Asn Lys Val Lys Ile Leu Trp Gly Val Gly Gln Leu Lys Lys 
                725                 730                 735     


Arg Leu Ser Glu Lys Ala Glu Lys Phe Gly Ile Gly Ile Val Pro Val 
            740                 745                 750         


Asn Pro Glu Leu Thr Ser Gln Met Asp Arg Glu Thr Phe Leu Leu Gly 
        755                 760                 765             


Tyr Arg Asn Pro Thr Asn Lys Lys Glu Leu Tyr Val Lys Arg Asp Asp 
    770                 775                 780                 


Lys Ile Glu Ile Leu Asp Ala Asp Glu Thr Ala Ser Tyr Asn Val Ala 
785                 790                 795                 800 


Leu Arg Gly Leu Gly His His Ala Asn Leu Ile Gln Phe Arg Ala Asp 
                805                 810                 815     


Lys Met Pro Asn Gly Cys Phe Arg Val Met Pro Asp Arg Lys Tyr Lys 
            820                 825                 830         


Gln Gly Ala Leu Tyr Gly Tyr Leu Asn Ser Thr Ala Val Leu Phe Lys 
        835                 840                 845             


Asp Lys Gly Asp Gly Val Leu Thr Ile His Lys Ser Lys Leu Thr Lys 
    850                 855                 860                 


Lys Glu Arg Asp Ser Arg Pro Ile Lys Gly Lys Lys Thr Phe Val Val 
865                 870                 875                 880 


Lys Asn Gly Lys Arg Trp Ile Leu Arg His Val Leu Asp Glu Glu Val 
                885                 890                 895     


Lys Lys Tyr Pro Glu Met Tyr Asn Ser Gln Asn 
            900                 905         


<210>  7
<211>  912
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.9İ

<400>  7

Met Ser Asp Tyr Lys Phe Ser Asn Asn Gly Val Thr Asn Thr Gly Ser 
1               5                   10                  15      


Ala His Ile Gly Leu Ser Pro Glu Asn Ser Ser Thr Val Met Asp Met 
            20                  25                  30          


Phe Lys Val Ile Thr Lys Asp Ala Asp Phe Leu Leu Lys Asn Leu Leu 
        35                  40                  45              


Ile Met Glu Gly Gly Glu Tyr Met Leu Asn Arg Glu Ile His Asn Gly 
    50                  55                  60                  


Asp Lys Glu Phe Asp Lys Ile Ile Ser Lys Leu Gly Leu Ser Lys Lys 
65                  70                  75                  80  


Glu Lys Glu Asn Leu Lys Met Lys Cys Lys Asp Phe Phe Phe Asp Phe 
                85                  90                  95      


Val Lys Leu Gln Asn Gly Arg Ser Leu Ala Asn Ile Leu Phe Glu Thr 
            100                 105                 110         


Lys Gly Thr Thr Leu Ile Gly Cys Gly Lys Asp Lys Lys Gly Glu Lys 
        115                 120                 125             


Val Asp Gly Glu Tyr Pro Thr Ile Tyr His Asp His Glu Thr Leu Arg 
    130                 135                 140                 


Ser Thr Gly Leu Leu Pro Leu Lys Phe Ser Lys Asn Ile Asp Asp Val 
145                 150                 155                 160 


Asp Tyr Lys Tyr Leu Ile Cys Tyr Leu Val His Asn Val Leu Ser Ser 
                165                 170                 175     


Phe Ile Glu Lys Arg Asp Ala Tyr Asn Asp Asn Lys Lys Glu Trp Glu 
            180                 185                 190         


Ser Lys Leu Ser Asn Ser Asn Leu Pro Gln Leu Glu Arg Met Ser Glu 
        195                 200                 205             


Phe Leu Asn Gly Ile Asn His Leu Gly Asn Ile Ile Gly Trp Asn Gly 
    210                 215                 220                 


Lys Lys Tyr Ile Gly Phe Ile Lys Lys Trp Thr Asp Glu Glu Ser Ser 
225                 230                 235                 240 


Met Tyr Asp Phe Phe Val Gln Lys Leu Gln Asp Asn Pro Lys Tyr Lys 
                245                 250                 255     


Phe Gly Lys Lys Asp Gln Phe Leu Tyr Gly Tyr Glu Pro Glu Phe Leu 
            260                 265                 270         


Asn Tyr Leu Phe His Asp Phe Arg Asp Leu Trp His Pro Asp Asn Leu 
        275                 280                 285             


Ile Gly Lys Asp Glu Tyr Val Asp Leu Ile Ser Gly Lys Asn Asn Thr 
    290                 295                 300                 


Asp Ala Glu Thr Ala Asn Lys Gly Ala Tyr His Trp Leu Lys Asp Phe 
305                 310                 315                 320 


Ile Asn Ile Ser Ser Phe Asp Ala Tyr Gly Lys Met Ala Thr Ile Gly 
                325                 330                 335     


Met Gly Asn Asn Leu Ile Asn Tyr Ser Met Asn Ile Asp Lys Asp Gly 
            340                 345                 350         


Lys Ile Ile Val Asn Met Asp Asn Ile Phe Asp Arg Ser Lys Pro Ile 
        355                 360                 365             


Val Phe Asn Val Tyr Arg Asn Ser Tyr Phe Arg Asn Phe Lys Ile Ile 
    370                 375                 380                 


Glu Ser Asp Asp Lys Lys Gly Ile Tyr Lys Val Glu Phe Ser Thr Ser 
385                 390                 395                 400 


Asn Asn Gly Val Ile Tyr Glu Gly Tyr Ile Lys Ser Pro Ser Leu Arg 
                405                 410                 415     


Phe Ala Thr Lys Gly Gly Thr Ile Lys Ile Asp Phe Pro Ile Ser Asp 
            420                 425                 430         


Lys Arg Ile Lys Gly Gly Arg Glu Met Asn Thr Asp Leu Met Trp Phe 
        435                 440                 445             


Leu Asn Arg Ala Ser Pro Cys Ser Thr Lys Asn Lys Glu Val Asn Ser 
    450                 455                 460                 


Phe Ile Gly Lys Asn Phe Val Gly Leu Ala Ile Asp Arg Gly Ile Asn 
465                 470                 475                 480 


Pro Leu Met Ala Trp Tyr Val Ala Glu Trp Thr Tyr Asp Lys Asp Gly 
                485                 490                 495     


Lys Ala Lys Ile Val Arg Ser Ile Ala Asn Gly Arg Val Asp Ser Gly 
            500                 505                 510         


His Asn Glu Ser Glu Val Lys Phe Val Arg Glu Thr Thr Asn Arg Ile 
        515                 520                 525             


Val Gly Ile Lys Ser Leu Val Trp Asn Thr Val Lys Tyr Arg Thr Gly 
    530                 535                 540                 


Gly Ser Glu Gly Ile Asp Arg Cys Arg Lys Ser Gln Asn Gly Gln Val 
545                 550                 555                 560 


Asp Leu Phe Glu Met Phe Asp Ile Asp Tyr Asn Asn Tyr Leu Lys Glu 
                565                 570                 575     


Val Asn Asn Leu Pro Tyr Asp Pro Asn Ser Glu Arg Ser Ile Ile Gln 
            580                 585                 590         


Thr Trp Val Ser Ser Pro Trp Lys Val Lys Asp Leu Val Lys Asp Ala 
        595                 600                 605             


Lys Asn Arg Met Val Gln Ile Lys Thr Gln Tyr His Asn Ala Lys Asp 
    610                 615                 620                 


Lys Glu Lys Tyr Ile Thr Thr Gln Asn Arg Ala Gly Phe Tyr Asp Phe 
625                 630                 635                 640 


Leu Lys Ile Glu Met Glu Lys Gln Phe Thr Ser Leu Gln Arg Met Phe 
                645                 650                 655     


Ser Gly Gly Gln Lys Asp Ile Cys Lys Asn Asn Glu Glu Tyr Arg Arg 
            660                 665                 670         


Gly Leu Arg Arg Arg Ile Asn Leu Tyr Thr Ser Ser Val Ile Met Ser 
        675                 680                 685             


Leu Ala Arg Lys Phe Asn Val Asp Cys Ile Phe Leu Glu Asp Leu Asp 
    690                 695                 700                 


Ser Ser Lys Ser Ser Trp Asp Asp Ala Lys Lys Asn Ser Leu Lys Asp 
705                 710                 715                 720 


Leu Trp Ser Thr Gly Gly Ala Asp Asp Ile Leu Gly Lys Met Ala Asn 
                725                 730                 735     


Lys Tyr Lys Tyr Pro Ile Val Lys Val Asn Ser His Leu Thr Ser Leu 
            740                 745                 750         


Val Asp Asn Lys Thr Gly Lys Ile Gly Tyr Arg Asp Pro Lys Lys Lys 
        755                 760                 765             


Ser Asn Leu Tyr Val Glu Arg Gly Lys Lys Ile Glu Ile Ile Asp Ser 
    770                 775                 780                 


Asp Glu Asn Ala Ala Ile Asn Ile Leu Lys Arg Gly Ile Ser Lys His 
785                 790                 795                 800 


Ile Asp Ile Arg Glu Phe Phe Ala Glu Lys Ile Glu Val Ser Gly Lys 
                805                 810                 815     


Thr Leu Tyr Arg Ile Ser Asn Lys Leu Gly Lys Gln Arg Met Gly Ser 
            820                 825                 830         


Leu Tyr Tyr Leu Glu Gly Asn Lys Glu Ile Leu Phe Gly Leu Gly Lys 
        835                 840                 845             


Asn Gly Glu Pro Ile Val Cys Lys Arg Gly Leu Cys Lys Lys Glu Arg 
    850                 855                 860                 


Leu Ala Pro Arg Ile Ala Glu Lys Lys Ser Thr Tyr Leu Ile Met Asn 
865                 870                 875                 880 


Gly Ser Lys Trp Met Phe Arg His Glu Ala Lys Lys Ile Val Glu Thr 
                885                 890                 895     


Tyr Lys Asp Arg Tyr Cys Ala Asn His Lys Val Ala Ser Lys Asp Gly 
            900                 905                 910         


<210>  8
<211>  1119
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.10İ

<400>  8

Met Met Asn Ile Asn Glu Met Val Lys Leu Met Lys Ser Glu Tyr Leu 
1               5                   10                  15      


Phe Glu Asp Asp Gly Ile Val Thr Lys Asn Lys Ile Gln Glu Arg Leu 
            20                  25                  30          


Arg Asn Gly Phe Ser Asp Ile Gly Val Asp Pro Ser Leu Val Ser Tyr 
        35                  40                  45              


Ala Ser Lys Phe Leu Asp Ser Met Phe Ile Cys Phe Ser Arg Val Lys 
    50                  55                  60                  


Gly Glu Lys Asn Phe Lys Ala Lys Asn Val Arg Lys Asn Met Ser Ser 
65                  70                  75                  80  


Ala Glu Lys Lys Ala Gln Lys Lys Lys Glu Tyr Gln Glu Tyr Tyr Gln 
                85                  90                  95      


Gly Val Met Ala Gln Gln Asp Ala Tyr Ala Gln Leu Leu Ser Asp Pro 
            100                 105                 110         


Thr Gln Glu Asn Leu Asp Lys Leu Asn Glu Leu Ile Ser Met Ser Val 
        115                 120                 125             


Asn Gly Ser Leu Val Glu Asp Phe Phe Pro Ala Leu Lys Asn Met Ile 
    130                 135                 140                 


Gln Lys Ala Asp Tyr Ser Ile Asp Lys Lys Gly Leu Leu Asp Phe Ser 
145                 150                 155                 160 


Cys Cys Met Met Asp Arg Tyr Glu Asp Arg Ser Leu Thr Arg Ala Ile 
                165                 170                 175     


Ser Ile Ser Ala Phe Asn Ile His Ser Gly Gly Leu Arg Lys Ala Leu 
            180                 185                 190         


Ser Asp Ile Ser Glu Lys Val Gln Asp Leu Ser Asn Thr Leu Leu Ile 
        195                 200                 205             


Arg Ile Leu Tyr Met Lys Gly Glu Glu Leu Ser Ile Asp Gly Glu Lys 
    210                 215                 220                 


Ile Ser Lys Glu Glu Val Gln Arg Gln Leu Lys Ala Asp Tyr Glu Glu 
225                 230                 235                 240 


His Lys Glu Tyr Phe Glu Asp Phe Glu Asp Phe Ala Lys Lys Cys Arg 
                245                 250                 255     


Phe Phe Tyr Asn Lys Phe Ser Lys Lys Lys Lys Thr Arg Gly Phe Gly 
            260                 265                 270         


Thr Tyr Phe Phe Gly Asp Lys Lys Lys Glu Ile Ser Ser Ala Glu Tyr 
        275                 280                 285             


Lys Ala His Lys Glu Leu Arg Asp Ser Gly Tyr Leu Trp Phe Asp Ile 
    290                 295                 300                 


Gly Trp Ser Glu Ser Ser Asp Phe Lys Tyr Val Ile Val Gly Asn Val 
305                 310                 315                 320 


Ser Gly Lys Leu Lys Ser Phe Glu Glu Thr Ser Glu Glu Tyr Gln Lys 
                325                 330                 335     


Ser Lys Asn Cys Trp Glu Ala Glu Arg Val Lys Leu Tyr Glu Gln Asp 
            340                 345                 350         


Ser Asp Phe Val Leu Phe Val Glu Asp Met Ile Glu Ser Lys Tyr Gly 
        355                 360                 365             


Pro Ile Glu Lys Met Lys Leu Arg Thr Phe Lys Thr Ile Val Lys Lys 
    370                 375                 380                 


Leu Asp Lys Glu Phe Gly Lys Arg Gly Asp Lys Thr Pro Ser Ile His 
385                 390                 395                 400 


Asp Tyr Phe Glu Ser Leu Asp Pro Asn His Thr Phe Ser Gln Ser Glu 
                405                 410                 415     


Gln Phe Met Tyr Gly Leu Asp Val Thr Leu Met Gln Phe Leu Phe Asn 
            420                 425                 430         


Asn Lys Lys Gln Phe Tyr Lys Leu Cys Lys Asp His Asp Gly Lys Arg 
        435                 440                 445             


Thr Phe Ala Lys Val Val Glu Glu Ser Tyr His Trp Gly Lys Asn Ser 
    450                 455                 460                 


Ile Asn Val Ser Thr Phe Gln Asn Ser Thr Ser Ile Leu Leu Gly Gly 
465                 470                 475                 480 


Asn Tyr Leu Asn Tyr Ser Met Ser Ile Glu Gly Glu Gly Leu Val Ile 
                485                 490                 495     


Lys Phe Asp Asn Pro Leu Ser Gly Lys Glu Val His Phe Val Val Cys 
            500                 505                 510         


Asn Asn Lys Tyr Leu Ser Asp Leu Glu Ile Leu Ser Gly Asn Pro Asn 
        515                 520                 525             


Arg Lys Asp Asn Asn Tyr Thr Ile Ser Tyr Ser Thr Gly Gly Lys Ala 
    530                 535                 540                 


Arg Phe Ile Ala Lys Ser Lys Glu Pro Arg Ile Phe Phe Asn Arg Lys 
545                 550                 555                 560 


Thr Lys Lys Trp Glu Ile Ala Phe Gln Leu Ser Asp Val Ser Pro Leu 
                565                 570                 575     


Asn Gly Lys Phe Gly Lys Gln Gly Glu Phe Leu Ser Asn Leu Arg Lys 
            580                 585                 590         


Phe Val Tyr Asn His Val Ala Lys Ser Pro Ser Lys Leu Asn Ile Ser 
        595                 600                 605             


Asp Asn Asn Cys Arg Ala Val Ala Tyr Asp Leu Gly Ile Arg Asn Val 
    610                 615                 620                 


Gly Ala Trp Ser Ser Phe Asp Phe Ser Tyr Lys Asp Gly Val Leu Gly 
625                 630                 635                 640 


Gly Tyr Lys Tyr Leu Thr Ser Gly Ser Leu Arg Ser Lys Ser Glu Ser 
                645                 650                 655     


Ser Glu Met Asp Gln Gly Tyr Tyr Phe Val Leu Asn Leu Lys Lys Ile 
            660                 665                 670         


Val Lys Leu Ile Pro Val Val Lys Lys Ser Ile Ile Asp Asp Pro Glu 
        675                 680                 685             


Leu Lys Arg Gln Phe Ile Gly Val Leu Asn Glu Asn Gly Asn Thr Val 
    690                 695                 700                 


Gly Leu Gly Asn Ile Gly Lys Leu Asp Ile Ala Ser Arg Lys Ala Val 
705                 710                 715                 720 


Gln Ser Phe His Asn Cys Ile Gln Gln Ile Asn Tyr Tyr Val Asp Thr 
                725                 730                 735     


Tyr Ala Asp His Ile Asp Lys Ile Ser Ala Lys Asp Phe Val Asp Asp 
            740                 745                 750         


Ile Asp Gly Ile Lys Val Leu Asp Glu Asp Asp Pro Tyr Val Val Lys 
        755                 760                 765             


Ile Leu Ser His Leu Pro Glu Asp Val Glu Gly Asn Gln Asp Asp Ile 
    770                 775                 780                 


Leu Asn Ile Ser Leu Leu Lys Trp Lys Thr Ser Asn Ala Gln Phe Val 
785                 790                 795                 800 


Pro Pro Leu Ile Gln Glu Ala Lys Ala Ile Met Ser Arg Ile Lys Arg 
                805                 810                 815     


Glu Asn Leu Asp Asn Ile Arg Gly Lys Lys Thr Gln Val Val Thr Gln 
            820                 825                 830         


Lys Thr Phe His Lys Ile Lys Phe Ala Lys Ala Leu Leu Ser Leu Met 
        835                 840                 845             


Lys Ser Trp Ser Ser Ile Gly Thr Val Arg Val Val Lys Thr Asp Gln 
    850                 855                 860                 


Ile Tyr Gly Lys Lys Ile Trp Asp Tyr Ile Asn Gly Leu Arg Arg Asn 
865                 870                 875                 880 


Val Leu Thr Tyr Leu Ser Ser Ala Ile Val Asn Asn Ala Leu Asp Leu 
                885                 890                 895     


Gly Ala His Met Ile Ile Leu Glu Asp Leu Asp Ser Ser Val Ser Lys 
            900                 905                 910         


Tyr Arg Glu Lys Asp Lys Asn Ala Ile Gln Ser Leu Trp Gly Ser Gly 
        915                 920                 925             


Glu Leu Lys Lys Arg Ile Glu Glu Lys Ala Glu Lys His Arg Val Val 
    930                 935                 940                 


Val Gln Tyr Val Ser Pro Tyr Leu Thr Ser Gln Leu Asp Asn Glu Thr 
945                 950                 955                 960 


Lys Asp Ile Gly Tyr Arg Lys Gly Gly Arg Leu Tyr Val Val Arg Asn 
                965                 970                 975     


Gly Lys Ile Lys Ser Ile Asp Ala Asp Ile Asn Ala Ser Lys Asn Ile 
            980                 985                 990         


Gly Glu Arg Phe Phe Asp Arg Asp  Leu Ile Gln Thr Leu  Ser Gly Val 
        995                 1000                 1005             


Val Val  Glu Asp Gln Ser Thr  Val Tyr Ile Leu Gln  Lys Arg Asn 
    1010                 1015                 1020             


Val Ser  Ser Asp Asn Arg Lys  Arg Phe Tyr Lys Lys  Phe Leu Glu 
    1025                 1030                 1035             


Asp Val  Gly Gly Lys Ser Lys  Lys Asp Ala Val Leu  Lys Met Gly 
    1040                 1045                 1050             


Asp His  Gly Glu Leu Glu Val  Glu Arg Leu Ile Asp  Gly Lys Lys 
    1055                 1060                 1065             


Leu Asp  Ile Asp Gly Lys Lys  Ile Leu Val Asp Gly  Glu Lys Val 
    1070                 1075                 1080             


Pro Phe  Arg Asn Thr Ser Val  Tyr Tyr Ser Pro Lys  Lys Lys Lys 
    1085                 1090                 1095             


Trp Val  Ser Lys Glu Leu Arg  Cys Asn His Ile Lys  Leu Thr Val 
    1100                 1105                 1110             


Glu Glu  Gln Asp Ile Lys 
    1115                 


<210>  9
<211>  1135
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.11İ

<400>  9

Met Asn Asn Tyr Asp Asn Tyr Leu Ser Asp Tyr Leu Ala Met Leu Pro 
1               5                   10                  15      


His Thr Lys Arg Thr Glu Ile Lys Lys Thr Ala Ser Lys Ile Ser Arg 
            20                  25                  30          


Lys Leu Asn Gln Lys Glu Val Lys Lys Gln Ile Glu Arg Ser Glu Tyr 
        35                  40                  45              


Ile Arg Ser Asn Cys Gly Tyr Ile Asn Ile Glu Arg Pro Gln Lys Ser 
    50                  55                  60                  


Leu Ser Phe Leu Ser Tyr Ser Thr Ile Lys Ser Ala Cys Met Ser Val 
65                  70                  75                  80  


Asn Phe Arg Ala Phe Gln Asn Pro Ile Asn Asp Tyr Glu Thr Ala Ile 
                85                  90                  95      


Cys Asn Gly Ile Asn Glu Cys Glu Arg Phe Phe Tyr Gln Gln Ile Asp 
            100                 105                 110         


Ser Ile Tyr Met Ser Gln Ile Ile Glu Gln Leu Phe Asp Phe Tyr Ile 
        115                 120                 125             


Ala Ser Arg Gln His Asp Met Phe Ile Asn Asn Thr Val Val Pro Tyr 
    130                 135                 140                 


Asp Val Asn Lys Leu Lys Ser Tyr Tyr Thr Ala Asn Glu Lys Tyr Ser 
145                 150                 155                 160 


Phe Glu Gln Phe Cys Asp Asp Ile Lys Glu Phe Thr Asn Lys Gly Phe 
                165                 170                 175     


Thr Ser Gly Gly Val Ser Cys Ile Leu Asn Leu Phe Tyr Lys Gly Ser 
            180                 185                 190         


Val Lys Asp Ser Lys Asn Lys Lys Asp Tyr Ile Lys Ser Val Lys Arg 
        195                 200                 205             


Leu Glu Thr Asn Gly Leu Phe Lys Lys Leu Asn Ile Phe Glu Lys Asn 
    210                 215                 220                 


Gly Ile Ser Lys Tyr Phe Ala Ala Ser Thr Leu Ser Thr Phe Phe Ala 
225                 230                 235                 240 


Thr Ile Ser Ser Trp Lys Lys Gln Asn Asp Asp Trp Thr Gly Val Ala 
                245                 250                 255     


Lys Asp Gly Thr Ser Leu Leu Ser Lys Leu Glu Asn Lys Thr Ile Thr 
            260                 265                 270         


Leu Gln Ser Ile Ile Lys His His Arg Val Ile Asn Glu Leu Ala Val 
        275                 280                 285             


Leu Ile Val Lys Ala Tyr Lys Asp Pro Val Lys Thr Leu Asn Asn Leu 
    290                 295                 300                 


Phe Glu Glu Arg Ser Asp Asn Asn Asn Asp Phe Lys Tyr Thr Cys Ser 
305                 310                 315                 320 


Asp Asp Glu Asp Lys Tyr Pro Met Tyr Ile Lys Arg Glu Ile Ala Glu 
                325                 330                 335     


Phe Val Lys Lys His Lys Thr Val Trp Glu Glu Ile Arg Tyr Phe Asp 
            340                 345                 350         


Glu Ser Asp Thr Lys Lys Lys Lys Arg Asp Lys Lys Glu Ser Ser Ser 
        355                 360                 365             


Asp Asp Lys Ser Tyr Leu Cys Cys Gly Asp Ser Trp Asp Tyr Leu Lys 
    370                 375                 380                 


Thr Trp Val Arg Leu Tyr Gly Glu Tyr Tyr Phe Phe Asp Asn Ala Leu 
385                 390                 395                 400 


Asn Gln Phe Leu Arg Lys Pro Ser Ala Ser Met His Leu Tyr Thr Ser 
                405                 410                 415     


Leu Asp Trp Ile Asn Lys Lys Thr Ile Cys Ile Val Gly Ala Asn Tyr 
            420                 425                 430         


Tyr Lys Ile Gly Lys Val Glu Val Val Glu Arg Asn Asn Gln Arg Phe 
        435                 440                 445             


Leu Leu Val Tyr Val Ser Val Pro Glu Met Glu Asn Tyr Ile Ile Ile 
    450                 455                 460                 


Pro Leu Gln Leu Asn Lys Tyr Phe Gly Asn Phe Gln Cys Lys Ile Phe 
465                 470                 475                 480 


Glu Gly Arg Leu Gln Ala Ile Phe Lys Arg Tyr Ala Asn Phe Asn Ala 
                485                 490                 495     


Leu Lys Asn Asn Lys Pro Gln Pro Ser Pro Asn Ile Ser Val Arg Ile 
            500                 505                 510         


Asn Glu Phe His Phe Ala Leu Arg Ser Tyr Arg Lys Gln Gln Ile Ser 
        515                 520                 525             


Ala Glu Asp Phe Ser Lys Gly Arg Phe Ser Leu Ile Ser Lys Ile Gly 
    530                 535                 540                 


Phe Gln Met Thr Asn Asp Glu Val Phe Gly Arg Thr Pro Arg Glu Ile 
545                 550                 555                 560 


Ala Leu Val Lys Asp His Leu Ser Lys Gly Tyr Val His Phe Gly Ser 
                565                 570                 575     


Gln Ile Ile Glu Asp Ser Arg Lys Glu Val Glu Gln Val Leu Lys Lys 
            580                 585                 590         


Pro Met Ile Leu Met Gly Val Asp Phe Gly Tyr Ser Pro Leu Ala Ser 
        595                 600                 605             


Tyr Asn Ile Lys Pro Leu Gln Thr Gly Lys Pro Ala Thr Asp Trp Val 
    610                 615                 620                 


Lys Asn Leu His Gly Asn Phe Leu Cys Gln Asn Val Ser Leu Gly Glu 
625                 630                 635                 640 


Thr Ile Thr Glu Gly Glu Ile Gly Asp Val Pro Thr Asp Thr Tyr Thr 
                645                 650                 655     


Ser Ser Asn Glu Ile Tyr Ser Ile Ala Thr Leu Thr Phe Arg Asn Ala 
            660                 665                 670         


Asp Gly Lys Leu Glu Asn Arg Ser Phe Ser Arg Phe Tyr His Glu Leu 
        675                 680                 685             


Asn Asn Thr Leu Asn Ile Ile Glu Gln Ile Lys Gly Thr Phe Asn Phe 
    690                 695                 700                 


Ile His Ser Ile Asn Thr Gln Phe Lys Glu Ile Lys Ala Leu Lys Thr 
705                 710                 715                 720 


Thr Glu Glu Phe Ser Ser Tyr Val Ser Thr Leu Thr Trp Asp Gln Phe 
                725                 730                 735     


Ile Glu Asp Ser Arg Lys Thr Ala Arg Tyr Ser Lys Tyr Trp Ile His 
            740                 745                 750         


Ile Ile Asn Glu Asn Pro Lys Arg Arg Thr Ile Ala Thr Leu Asn Glu 
        755                 760                 765             


Thr Leu Lys Leu Val Asp Glu Lys His Arg Phe Thr Val Thr Ile Gln 
    770                 775                 780                 


Glu Ile Phe Asp Leu Val Lys Tyr Cys Gln Gln His Gly Tyr Tyr Pro 
785                 790                 795                 800 


Lys Ser Asn Val Met Ser Lys Leu Arg Asn Leu Ala Ile Lys Leu Ile 
                805                 810                 815     


Asn Asp Leu Ile Arg Tyr Gln Lys Ile Gly Ile His Ser Cys Tyr Leu 
            820                 825                 830         


Asp Phe Cys Val Leu Ile Lys Asn His Ile Ala Leu Leu Asn Ser Ser 
        835                 840                 845             


Thr Ala Phe Ile Ile Asn Phe Ser Arg Asn Lys Glu Asn Ile Ile Arg 
    850                 855                 860                 


Asn Asn Thr Ser Lys Ile His Ser Leu Trp Val Tyr Arg Asp Asn Phe 
865                 870                 875                 880 


Arg Arg Gln Met Ile Lys Asn Leu Cys Ser Gln Ile Leu Lys Ile Ala 
                885                 890                 895     


Ala Lys Asn Lys Val His Ile Val Val Val Glu Lys Leu Asn Asn Met 
            900                 905                 910         


Arg Thr Asn Asn Arg Asn Asn Glu Asp Lys Asn Asn Met Ile Asp Leu 
        915                 920                 925             


Leu Ala Thr Gly Gln Phe Arg Lys Gln Leu Ser Asp Gln Ala Lys Trp 
    930                 935                 940                 


Tyr Gly Ile Ala Val Val Asp Thr Ala Glu Tyr Asn Thr Ser Lys Val 
945                 950                 955                 960 


Asp Phe Met Thr Gly Glu Tyr Gly Tyr Arg Asp Glu Asn Asn Lys Arg 
                965                 970                 975     


His Phe Tyr Cys Arg Lys Gln Asp Lys Thr Val Leu Leu Asp Cys Asp 
            980                 985                 990         


Lys Lys Ala Ser Glu Asn Ile Leu  Leu Ala Phe Val Thr  Gln Ser Leu 
        995                 1000                 1005             


Leu Leu  Asn His Leu Lys Val  Leu Ile Thr Glu Asp  Gly Lys Thr 
    1010                 1015                 1020             


Ala Val  Ile Asp Leu Ser Glu  Arg Thr Thr Glu Pro  Gln Lys Ile 
    1025                 1030                 1035             


Arg Ser  Lys Ile Trp Thr Asn  Ser Asp Val Gln Lys  Ile Ile Phe 
    1040                 1045                 1050             


Cys Lys  Gln Glu Asn Gly Ser  Tyr Val Leu Lys Lys  Gly Ser Thr 
    1055                 1060                 1065             


Asp Ile  Lys Glu Lys Met His  Lys Ala Val Leu His  Arg His Gly 
    1070                 1075                 1080             


Ser Leu  Trp Tyr Asp Tyr Leu  Asn His Lys Asn Met  Ile Glu Asp 
    1085                 1090                 1095             


Ile Lys  Asn Leu His Leu Ser  Asn Cys Ser Leu Thr  Thr Ser Thr 
    1100                 1105                 1110             


Asn Ser  Asp Val Ile Asn Ser  His Ser Gly Ser Ser  Arg Ser Leu 
    1115                 1120                 1125             


Asp Lys  Thr Lys Thr Tyr Ala  
    1130                 1135 


<210>  10
<211>  1013
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.12İ

<400>  10

Met Ala Ser Ser Asp Ala Gln Lys Phe Pro Gln Thr His Asn Lys Val 
1               5                   10                  15      


Met Ser Phe Arg Leu Thr Ala Ser Asn Ile Gly Ser Val Leu Ser Leu 
            20                  25                  30          


His Ser Asn Leu His Asp Ala Ala Glu Ile Gly Ile Asn Glu Cys Arg 
        35                  40                  45              


Trp Trp Ile Gly Asp Gly Glu Ile Tyr Glu Arg Asp Pro Ala Cys Arg 
    50                  55                  60                  


Ser Ile Lys Lys Gly Asn Asp Ile Arg Thr Val Thr Ser Glu Lys Ile 
65                  70                  75                  80  


Lys Glu Leu Trp Thr Lys His Thr Asp His Ser Val Pro Leu Val Asp 
                85                  90                  95      


Phe Ile Asp Met Leu Lys Phe Val Ala Gln Cys Ala Ile Tyr Gly Asp 
            100                 105                 110         


Ser Arg Ala Leu Ala Ser Thr Leu Phe Gly Lys Ser Lys Ala Glu Thr 
        115                 120                 125             


Arg Gly Val Ser Thr Glu Asp Met Thr Val Ile Arg Ala Trp Ile Ala 
    130                 135                 140                 


Glu Thr Asp Ala Val Leu Ala Ser Gly Leu Ser Pro Lys Lys Lys Lys 
145                 150                 155                 160 


Lys Lys Glu Lys Glu Ala Gly Lys Lys Glu Arg Lys Pro Asp Val Lys 
                165                 170                 175     


Met Glu Met Cys Arg Arg Ile Arg Cys Thr Met Val Gln Cys Gly Tyr 
            180                 185                 190         


Phe Arg Arg Phe Pro Phe Glu Ala Lys Ile Asp Asn Gly Gly Glu Arg 
        195                 200                 205             


Gly Lys Met Asp Ser Glu Leu Ser Tyr Val Ser Ala Arg Asn Leu Leu 
    210                 215                 220                 


Arg Cys Leu Ser Thr Trp Arg Ala Ser Ser Val Met Arg Arg Asp Ser 
225                 230                 235                 240 


Tyr Leu Ile Glu Glu Glu Arg Ile Lys Glu Ala Glu Ser Lys Met Thr 
                245                 250                 255     


Pro Glu Ile Ile Asp Gly Leu Arg Arg Leu Tyr Arg Tyr Cys Ala Val 
            260                 265                 270         


Asp His Asp Phe Leu Lys Trp Phe Gly Gly Arg Ile Ile Arg His Ile 
        275                 280                 285             


Asp Ser Cys Leu Ala Pro Ala Ile Ala Gly Asn Thr Gly Arg Pro Thr 
    290                 295                 300                 


Gly Gly Glu Ser Phe Thr Val Ile Tyr Asp Arg Arg Lys Lys Arg Asp 
305                 310                 315                 320 


Val Lys Ile Thr Tyr Ser Val Pro Glu Glu Ile Tyr Gly Tyr Leu Ser 
                325                 330                 335     


Ser His Pro Glu Leu Val Ala Ile Gly Lys Asp Gly Met Thr Pro Ile 
            340                 345                 350         


Ser Arg His Ala Asp Tyr Leu Glu Met Ile Ala Ser His Glu Lys His 
        355                 360                 365             


Arg Trp Tyr Ala Thr Phe Pro Thr Val Gly Lys Glu Asp Gly Tyr Arg 
    370                 375                 380                 


Thr Ser Val Leu Leu Gly Lys Asn Tyr Leu Thr Tyr Asp Leu Ser Tyr 
385                 390                 395                 400 


Asp Gly Glu Ser Val Pro Asp Lys Lys Ile Asn Val Ile Ser Lys Gly 
                405                 410                 415     


Gln Pro Val Cys Leu Asp Leu His Asp Gly Arg Arg Val Ser Ser Leu 
            420                 425                 430         


Tyr Leu Thr Val Gly Glu Ser Ala Ala Tyr Asp Ile Ala Val Arg Lys 
        435                 440                 445             


Asn Lys Arg His His Gly Lys Pro Ala Asp Tyr Cys Arg Met Arg Val 
    450                 455                 460                 


His Leu Thr Gln Glu Arg Glu Asp Lys Thr Tyr Asn Asp Pro Tyr Phe 
465                 470                 475                 480 


Ser Asn Met Glu Ile Trp Arg Ala Gly Asp Gln Val Tyr Ala Ile Glu 
                485                 490                 495     


Phe Asp Arg His Gly Ala Arg Tyr Thr Ala Ile Val Lys Glu Pro Ser 
            500                 505                 510         


Val Glu Tyr Arg Asn Lys Lys Leu Tyr Leu Arg Val Asn Met Val Leu 
        515                 520                 525             


Asp Ser Pro Ser Arg Gln Asp Asp Lys Asp Met Tyr Tyr Ala Tyr Met 
    530                 535                 540                 


Thr Ala Tyr Pro Ser Ser Asn Pro Pro Val Glu Thr Ser Asp Asn Lys 
545                 550                 555                 560 


Lys Arg Phe Glu Arg Leu Gly Pro Gly Arg Arg Ala Ile Gly Gly Ile 
                565                 570                 575     


Asp Ile Gly Ile Gly Arg Pro Tyr Val Ala Val Val Ala Ser Tyr Glu 
            580                 585                 590         


Val Gly Pro Ala Gly Thr Glu Gln Lys Phe Gln Ile Glu Asp Arg Leu 
        595                 600                 605             


Ile Glu Asp Asp Gly Ser Ser Pro Tyr Asp Ser Leu Tyr Asn Asp Phe 
    610                 615                 620                 


Leu Thr Asp Ile Arg Thr Val Ser Arg Ile Ile Glu Ala Ala Lys Lys 
625                 630                 635                 640 


Ile Ser Glu Gly Asp Leu Glu Asp Ile Pro Ser Asp Met Ser Val Asp 
                645                 650                 655     


Glu Asp Gly Ser Ile Ala Ala Thr Met Lys Arg Met Ser Ala Arg Ile 
            660                 665                 670         


Ala Glu Arg His His Leu Tyr Gly Glu Arg Lys Ser Glu Ala Tyr Ala 
        675                 680                 685             


Thr Phe Leu Lys Met Asn His Lys Gln Arg Leu Asp Ile Leu Leu Thr 
    690                 695                 700                 


Gln Lys Ala Ser Asn Ala Thr Leu Lys Gln Leu Val Glu Glu Asp Pro 
705                 710                 715                 720 


Ser Phe Leu Pro Arg Ile Cys Val Tyr Tyr Val Ile Ser Val Glu Arg 
                725                 730                 735     


Glu Leu Lys Asn Lys His Arg Asn Ala Tyr Leu Asp Gly Leu Thr Val 
            740                 745                 750         


Asp Glu Lys Tyr Ser Gly Glu Thr Lys Arg Gly Tyr Ala Gln Lys Arg 
        755                 760                 765             


Leu Asn Ser Met Leu Arg Ala Tyr Ser Ala Leu Gly Glu Glu Glu Thr 
    770                 775                 780                 


Asp Glu Val Arg Thr Phe Ser Thr Arg Ser Glu Lys Val Arg Asn Met 
785                 790                 795                 800 


Ala Lys Asn Ala Ile Lys Arg Asn Ala Arg Lys Leu Val Asn Phe Tyr 
                805                 810                 815     


Val Gly Lys Gly Ile Arg Thr Ile Val Ala Glu Asp Thr Asp Pro Thr 
            820                 825                 830         


Lys Ser Arg Asn Asp Gly Lys Lys Ser Asn Arg Ile Lys Ala Ala Trp 
        835                 840                 845             


Ser Pro Lys Gln Phe Leu Ala Ala Val Lys Asn Ala Ala Gln Trp His 
    850                 855                 860                 


Gly Leu Glu Ile Ala Glu Val Asp Pro Arg Met Thr Ser Gln Val His 
865                 870                 875                 880 


Pro Glu Thr Gly Leu Ile Gly Tyr Arg Asp Gly Asp Thr Leu His Cys 
                885                 890                 895     


Pro Asp Gly Ser Lys Ile Asp Ala Asp Val Ala Gly Ala Ala Asn Val 
            900                 905                 910         


Cys Arg Val Phe Ala Gly Arg Gly Leu Trp Arg Phe Ser Ile Asn Thr 
        915                 920                 925             


Asn Ile Asp Ile Ser Asn Lys Asp Glu Lys Lys Arg Leu Arg Ala Tyr 
    930                 935                 940                 


Ile Val His His Phe Gly Ser Glu Ser Asn Trp Glu Lys Phe Arg Lys 
945                 950                 955                 960 


Gln Tyr Pro Ser Gly Thr Thr Leu Tyr Leu His Gly Arg Glu Trp Leu 
                965                 970                 975     


Thr Ala Glu Glu His Lys Ser Ala Ile Asp Arg Ile Arg Asp Asp Val 
            980                 985                 990         


Gly Arg Asp Ala Glu Asn Asp His  Val Ala Ile Val Thr  Ala Ala Glu 
        995                 1000                 1005             


Lys Val  Glu Ile Phe 
    1010             


<210>  11
<211>  1052
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.13İ

<400>  11

Met Ser His Asp Leu Lys Pro Gln Arg Leu Ile Arg Ser Asn Ile Thr 
1               5                   10                  15      


Lys Thr His Ser Asp Gln Asn Ala Lys Gln Val Ala Glu Glu Val Lys 
            20                  25                  30          


Lys Glu His Leu Asn Tyr Leu Leu Ile Lys Asn Glu Met Leu Ile Ser 
        35                  40                  45              


Ile Val Pro Glu Ala Lys Asp Asp Asp Gly Asn Asp Ile Asp Phe Lys 
    50                  55                  60                  


Lys Gln Leu Lys Ser Leu Tyr Lys Glu Thr Asp Gln Ser Val Ser Phe 
65                  70                  75                  80  


Ser Val Phe Cys Gln Met Met Lys Phe Arg Asn Ile Ala Leu Leu Tyr 
                85                  90                  95      


Ala Lys Gly Gln Ser Arg Trp Ala Val Ser Ser Tyr Phe Thr Gly Asn 
            100                 105                 110         


Arg Arg Lys Asp Asp Tyr Ala Lys Asp Leu Ser Leu Leu Asp Glu Ala 
        115                 120                 125             


Ile Glu Leu Leu Glu Cys Lys Arg Arg Lys Lys Ala Glu Glu Glu Asn 
    130                 135                 140                 


Glu Glu Glu Asn Glu Thr Pro Lys Lys Lys Glu Asp Asn Pro Ser Asn 
145                 150                 155                 160 


Ile Ser Glu Glu Gln Ile Met Lys Leu Phe Tyr Ala Val Asn Lys Lys 
                165                 170                 175     


Leu Lys Glu Ile Gly Tyr Leu Asp Arg Tyr Ser His Ile Glu Lys Gln 
            180                 185                 190         


Glu Gln Tyr Ala Ile Ile Gly Val Thr Ser Arg Thr Val Lys Ala Trp 
        195                 200                 205             


Asp Tyr Ala Asn Phe Ala Thr Arg Asn His Tyr Gln Ser Val Gln Asn 
    210                 215                 220                 


Glu Tyr Gln Lys Lys Leu Lys Ala Leu Pro Gly Thr Lys Lys Asp Lys 
225                 230                 235                 240 


Val Cys Leu Glu Lys Phe Phe Asp His Leu Asn Glu Asn Asn Ile Ala 
                245                 250                 255     


Ala Asp Trp Asp Lys Trp Arg Leu Lys Lys His Ile Leu Gln Cys Ile 
            260                 265                 270         


Ile Pro Ala Ala Lys Ile Gly Leu Lys Glu Leu Lys Gln Ser Phe Tyr 
        275                 280                 285             


Val Asp Asn Lys Gly Asn Lys His Asn Tyr Phe Val Asn Gly Leu Tyr 
    290                 295                 300                 


Glu Glu Ile Leu Lys Arg Pro Phe Leu Tyr Ser Ala Glu Asp Pro Glu 
305                 310                 315                 320 


Glu Ser Ile Leu Tyr Leu Gly Val Glu Val Ala Ser Leu His Ser Lys 
                325                 330                 335     


Leu Asn His Leu Arg Ser Glu Ala Arg Phe Ser Phe Glu Thr Pro Asp 
            340                 345                 350         


Asp Ile Cys Lys Tyr Met Thr Ile Cys Gly Asp Asn Tyr His Asn Phe 
        355                 360                 365             


Thr Met Ser Ala Ile Gly Glu Asp Val Glu Asp Ile Glu Val Glu Val 
    370                 375                 380                 


Tyr Asp Tyr Asn His Ser Lys Lys Tyr Glu Thr Met Arg Phe Ile Asn 
385                 390                 395                 400 


Gly Lys Arg Thr Thr Asp Leu Ser Leu Asn Phe Lys Gly Ile Pro Val 
                405                 410                 415     


Arg Leu Cys Leu Glu Gly Lys Arg Asn Asn Ser Tyr Phe Ala Asp Ala 
            420                 425                 430         


Ile Val Trp Glu Leu Asp Asn Lys Asp Lys Thr Gly Tyr Leu Ile Glu 
        435                 440                 445             


Tyr Gly Lys Ser Asn Asn Arg Leu Tyr Met Leu Val Lys Glu Pro Leu 
    450                 455                 460                 


Ile Gly Cys Arg Arg Lys Phe Gly Lys Asp Val Leu Phe Val Ser Leu 
465                 470                 475                 480 


Ser Gly Thr Leu Val Asn Lys Tyr Ile Glu Asp Asp Ile Val Ser Ala 
                485                 490                 495     


Arg Tyr Leu Met Gln Thr Ala Ala Pro Ile Phe Lys Thr Ser Arg Ala 
            500                 505                 510         


Lys Lys Gln Asp Lys Ile Gly Asp Lys Trp Phe Glu His Cys Gln Gly 
        515                 520                 525             


Ser Thr Ile Lys Ile Ala Gly Ile Asp Ile Gly Ile Asn Pro Ile Ala 
    530                 535                 540                 


Ala Ile Thr Val Ala Asn Val Thr Phe Asp Arg Ala Leu Gly Asn Lys 
545                 550                 555                 560 


Ile Lys Asn Gln Lys Gln Ile Val Ile Asp Cys Tyr Ala Glu Asp Tyr 
                565                 570                 575     


Lys Ile Asp Pro Val Val Val Lys Arg Met Glu Asp Ile Arg His Ile 
            580                 585                 590         


Lys Tyr Thr Ile Asn Ser Trp Tyr His Leu Ala Asp Cys Cys Arg Leu 
        595                 600                 605             


Lys Ala Ala Asn Lys Glu Tyr Val Val Asn Glu Arg Lys Gln Gly Phe 
    610                 615                 620                 


Phe Arg Glu Asn Ile Glu Tyr Leu Lys Glu Val Ala Lys Lys Ala Ile 
625                 630                 635                 640 


Thr Glu Ser Asp Gln Gln Ile Lys Glu Gln Lys Ala Ala Leu Lys Arg 
                645                 650                 655     


Phe Asp Gly Glu Lys Lys Lys Glu Ile Gln Ala Thr Ile Asn Gly Phe 
            660                 665                 670         


Asn Leu Lys Ile Lys Ile Leu Lys Lys Phe Val Arg Gln Ser Ala Lys 
        675                 680                 685             


Lys Ile Phe Asp Ser Thr Leu Glu Thr Leu Glu Lys Tyr Asp Asn Asn 
    690                 695                 700                 


Ile Glu Gln Ala Lys Arg Asp Arg Glu Phe Gly Leu Lys Ile Ile Tyr 
705                 710                 715                 720 


Asp Leu Ile Ile Lys Tyr Tyr Lys Arg Ser Lys Lys Glu Arg Glu Met 
                725                 730                 735     


Asn Gln Arg Ile Tyr Val Asp Asp Tyr Asn Gln Glu Glu Ile Asp Thr 
            740                 745                 750         


Glu Arg Thr Lys Lys Ile Arg Lys Glu Thr Ile Thr Phe Cys Asp Asn 
        755                 760                 765             


Asp Trp Asn Ser Leu Thr Lys Arg Ile His Asp Leu Glu Lys Lys Met 
    770                 775                 780                 


Lys Lys Ile Gly Ile Ser Glu Pro Gly Arg Val Glu Gln Glu Ile Asn 
785                 790                 795                 800 


Asp Arg Asp Tyr Tyr Asn Asn Ile Gln Asp Asn Thr Lys Lys Arg Gln 
                805                 810                 815     


Ala Lys Ile Ile Val Asp Ala Leu Lys Glu Glu Gly Val Ser Ile Ile 
            820                 825                 830         


Val Val Glu Asp Leu Thr Gly Gly Gly Ser Glu Asn Thr Lys Glu Ile 
        835                 840                 845             


Asn Lys Ser Phe Asp Ala Phe Ala Pro Ile Arg Phe Leu Asn Ala Leu 
    850                 855                 860                 


Lys Asn Cys Ala Glu Thr Asn Gly Ile Gln Val Thr Glu Val Leu Ser 
865                 870                 875                 880 


Pro Met Ser Ser Lys Met Val Pro Ser Thr Gly Glu Ile Gly His Arg 
                885                 890                 895     


Asp Lys Arg Asp Lys Gln Leu Tyr Tyr Lys Asp Gly Glu Glu Leu Lys 
            900                 905                 910         


Ser Ile Asp Gly Asp Ile Ser Ala Ser Glu Ile Leu Leu Arg Arg Gly 
        915                 920                 925             


Val Ser Arg His Thr Glu Leu Ile Gly Thr Met Asn Val Glu Asp Val 
    930                 935                 940                 


Leu Asp Lys Asn Asn Asn Lys Asn Lys Cys Ile Lys Gly Tyr Val Cys 
945                 950                 955                 960 


Asn Arg Trp Gly Asn Ile Gln Asn Phe Glu Lys Ile Leu Lys Glu Lys 
                965                 970                 975     


Gly Ile Gly Glu Arg Glu Ile Ile Tyr Leu His Gly Asp Lys Ile Leu 
            980                 985                 990         


Thr Met Asp Glu Lys Arg Thr Leu  Gln Ala Ser Ile Arg  Lys Glu Leu 
        995                 1000                 1005             


Lys Glu  Met Arg Glu Arg Glu  Ser Gly Glu Glu Asn  Ala Gly Thr 
    1010                 1015                 1020             


Ala Arg  Lys Lys Ser Lys Pro  Lys Lys Lys Lys Lys  Ile Lys Arg 
    1025                 1030                 1035             


Asn Asn  Asp Gln Asp Leu Ser  Asn Asn Arg Pro Ala  Ala Ser 
    1040                 1045                 1050         


<210>  12
<211>  1045
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.14İ

<400>  12

Met Lys Glu Asn Lys Met Lys Glu Asn Gly Ser Met Thr Thr His Ser 
1               5                   10                  15      


Lys Val Ile Ala Leu Lys Met Lys Ser Glu Asn Val Glu Phe Asp Thr 
            20                  25                  30          


Phe Tyr Lys Glu Ser Phe Glu Leu Phe Lys Gln Phe Thr Asn Glu Phe 
        35                  40                  45              


Val Ala Trp Gly Asn Asp Glu Ile Tyr Gln Tyr Gly Ser Ser Lys Arg 
    50                  55                  60                  


Lys Lys Asp Asp Gln Lys Ile Ser Leu Ile Pro Val Ile Glu Asp Ile 
65                  70                  75                  80  


Tyr Lys Ser Val Glu Lys Lys Ala Thr Ala Glu Gly Ile Ser Lys Thr 
                85                  90                  95      


Asp Phe Arg Ala Val Leu Lys Tyr Leu Tyr His Gln Ile Ile Asn Val 
            100                 105                 110         


Gly Asn Ser Gly Arg Ser Tyr Gly Thr Ser Leu Phe Gly Gly Cys Glu 
        115                 120                 125             


Val Lys Glu Lys Leu Ser Lys Gln Asp Ile Ser Asn Ile Val Glu Cys 
    130                 135                 140                 


Val Lys Glu Leu Glu Leu Cys Lys Ser Lys Gln Glu Glu Ser Asp Ala 
145                 150                 155                 160 


Tyr Asp Lys Ile Leu Leu Lys Glu Lys Ile Thr His Ile Val Lys Ser 
                165                 170                 175     


Gly Glu Thr Ala Gly Asp Ile Thr Lys Lys Tyr Asn Gln Ala Thr Thr 
            180                 185                 190         


Gly Arg Lys Thr Ser Ser Lys Gly Phe Phe Asp Lys Ser Thr Lys Thr 
        195                 200                 205             


Glu Val Lys Tyr Lys Asp Ile Lys Asp Asp Thr Leu Leu Gln Asp Gly 
    210                 215                 220                 


Ser Thr Ile Phe Ile Lys Ser Ser Val Asp Leu Phe Val Lys Lys Val 
225                 230                 235                 240 


Cys Asn Thr Leu Arg Glu Ile Asn Phe Phe Asp Arg Leu Pro Phe Lys 
                245                 250                 255     


Asn Asn His Ser Asn Asn Tyr Gly Leu Leu Phe Ser Met Leu Ser Gln 
            260                 265                 270         


Ile Glu Ser Trp Lys Thr Ile Ser Glu Thr Thr Lys Lys Ser His Glu 
        275                 280                 285             


Glu His Gly Glu Lys Ile Ala Ser Met Val Lys Lys Leu Asp Leu Thr 
    290                 295                 300                 


Gln Thr Glu Leu Met Lys Asp Phe Ala Ala Phe Cys Ile Glu Asn Asn 
305                 310                 315                 320 


Ile Thr Lys Lys Phe Asp His Lys Phe Lys Arg His Met Glu Asp Cys 
                325                 330                 335     


Val Ile Pro Ser Phe Lys Asn Gly Lys Ile Pro Asp Lys Leu Phe Tyr 
            340                 345                 350         


Phe Asn Ile Ile Leu Ala Lys Lys Thr Asp Glu Gln Ile Asp Tyr Ser 
        355                 360                 365             


Leu Ser Ser Glu Phe Tyr Thr Lys Leu Phe Ser Met Pro Asn Leu Trp 
    370                 375                 380                 


Gln Glu Glu Glu Ala Phe Ile Val Lys Asn Ile Asn Leu Ile Glu Glu 
385                 390                 395                 400 


Ile Thr Ile Phe Asn Lys Arg Arg Asn Tyr Ala Cys Cys Pro Leu Ile 
                405                 410                 415     


Lys Glu Lys Glu Tyr Asp Arg Phe Gln Ile Gln Leu Asn Glu Thr Asn 
            420                 425                 430         


Phe Leu Lys Phe Gln Phe Asp Pro Lys Asn Val Val Asn Ile Asp Glu 
        435                 440                 445             


Asn Thr Thr Glu Ala Thr Val Gly Phe Asp Glu Lys Leu Lys Leu Val 
    450                 455                 460                 


Val Cys Ala Asp Lys Lys Tyr Ala Phe Ser Ile Phe Thr Gln Cys Lys 
465                 470                 475                 480 


Tyr His Gly Asn Lys His Lys Pro Asn Thr Tyr Phe Asn Asn Leu Lys 
                485                 490                 495     


Ile Ile Lys Val Ile Glu Ser Lys Ser Asn Ser Val Lys Ser Met Lys 
            500                 505                 510         


Tyr Thr Phe Glu Phe Thr Lys Arg Asn Glu Leu Lys Arg Ala Glu Ile 
        515                 520                 525             


Lys Gln Pro Ser Ile Val Tyr Lys Asn Asn Asn Tyr Tyr Ile Arg Ile 
    530                 535                 540                 


Asn Met Asn Val Ile Leu Asp Ala Asp Gln Thr Ser Tyr Lys Ile Ile 
545                 550                 555                 560 


Asn Asn Asn Gln Thr Ala Ser Leu Pro Ser Tyr Phe Gln Ser Ser Leu 
                565                 570                 575     


Pro Phe Glu Asn Asn Arg Gly Lys Ile His Asp Lys Gly Ile Val His 
            580                 585                 590         


Trp Glu Lys Ile Lys Asn Arg Lys Ile Ile Ala Met Gly Val Asp Leu 
        595                 600                 605             


Gly Val Arg Arg Pro Phe Ser Tyr Ala Ile Gly Asn Phe Thr Leu Asn 
    610                 615                 620                 


Lys Asp Ile Leu Asp Lys Asn Asp Val Asn Ile Val Ala Ser Gly Phe 
625                 630                 635                 640 


Asn Leu Cys Ser Asp Ser Asp Val Tyr Phe Gln Val Phe Asn Gln Ile 
                645                 650                 655     


Lys Thr Leu Ala Lys Phe Ile Gly Lys Leu Lys Ser His Asn Lys Gly 
            660                 665                 670         


Leu Lys Val Asp Phe Glu Lys Asp Lys Lys Tyr Ile Phe Asp Leu Val 
        675                 680                 685             


Asn Asp Ala Lys Ala Tyr Phe Lys Asp Met Ser Ala Lys Arg Ile Asn 
    690                 695                 700                 


Asp Thr Lys Asp Asn Ile Ser Asn Thr Val Thr Asn Lys Glu Arg Ile 
705                 710                 715                 720 


Tyr Gly Ser Phe Val Ser Glu Ser Ala Glu Ser Ala Ile Gln Cys Ala 
                725                 730                 735     


Ile Asp Arg Ser Glu Lys Glu Ser Gly Leu Thr Leu Lys Lys Asp Ile 
            740                 745                 750         


Ser Trp Leu Val Asn Val Leu Ser Lys Tyr Leu Glu Arg Lys Phe Lys 
        755                 760                 765             


Glu Val Lys Asn Asn Arg Lys Tyr Thr Asn Val Asn Lys Cys Asp Asn 
    770                 775                 780                 


Cys Phe Asn Trp Leu Arg Val Ile Glu Asn Ile Lys Arg Leu Lys Arg 
785                 790                 795                 800 


Ser Ile Ser Tyr Leu Gly Glu Asp Leu Gln Lys Asn Pro Glu Leu Lys 
                805                 810                 815     


Ile Glu Leu Lys Asn Leu Asn Glu Tyr Gly Asn Asn Val Lys Ser Asp 
            820                 825                 830         


Phe Leu Lys Gln Ile Ala Ser Asn Ile Ile Lys Val Ala Ile Glu His 
        835                 840                 845             


Lys Cys Asp Ile Val Phe Ile Glu Lys Leu Gly Lys Ala Asp Ser Arg 
    850                 855                 860                 


Ser Arg Lys Leu Asn Glu Met Phe Ser Phe Trp Ser Pro Lys Ala Ile 
865                 870                 875                 880 


Lys Lys Ala Ile Glu Asn Ala Ala Ser Trp His Gly Ile Pro Val Val 
                885                 890                 895     


Glu Val Asp Pro Ser Cys Thr Ser Lys Val His Tyr Glu Thr Asn Leu 
            900                 905                 910         


Phe Gly His Arg Ile Gly Asn Asp Leu Tyr Tyr Val Glu Asp Gln Cys 
        915                 920                 925             


Leu Lys Lys Val Asp Ala Asp Ile Asn Ala Ala Lys Gln Ile Leu Val 
    930                 935                 940                 


Arg Gly Ala Thr Arg His Gly Asn Ile Ser Ser Ile Asn Ile Lys Tyr 
945                 950                 955                 960 


Leu Gln Ala Lys Ile Ala Glu Leu Asn Ser Glu Ala Asn Ser Glu Glu 
                965                 970                 975     


Asp Lys Glu Glu Ile Lys Gln Gly Gly Lys Arg Ile Gln Gly Phe Leu 
            980                 985                 990         


Trp Lys Lys Tyr Gly Asn Ile Thr  Asn Ile Thr Asn Gln  Leu Thr Ala 
        995                 1000                 1005             


Ala His  Lys Glu Arg Glu Ser  Lys Phe Asp Tyr Ile  Tyr Leu His 
    1010                 1015                 1020             


Asn Asp  Lys Trp Ile Ala Tyr  Glu Asp Arg Asn Glu  Ile Lys Lys 
    1025                 1030                 1035             


Asp Ile  Glu Lys Arg Leu Glu  
    1040                 1045 


<210>  13
<211>  895
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.15İ

<400>  13

Met Thr Ala Lys Lys Thr Ala Lys Lys Tyr Phe Pro Pro Lys Cys Leu 
1               5                   10                  15      


Arg Ser Ser His Phe Lys Ile Tyr Gly Ile Pro Thr Ala Ile Arg Ala 
            20                  25                  30          


Leu Glu Glu Thr Asn Thr Phe Val Asn Lys Ala Ala Ala Asp Leu Met 
        35                  40                  45              


Glu Met Phe Phe Leu Met Arg Gly Gln Pro Tyr Arg Arg Arg Ile Gly 
    50                  55                  60                  


Ser Glu Glu Lys Gln Val Thr Gln Glu His Ile Asp Ala Arg Leu Arg 
65                  70                  75                  80  


Val Leu Val Gly Asp Tyr Ser Leu Asn Glu Val Lys Pro Leu Leu Arg 
                85                  90                  95      


Gln Leu Tyr Asp Gly Ile Lys Ala Lys Gln Asn Tyr Ala Pro Thr His 
            100                 105                 110         


Phe Val Arg Phe Phe Ile Gln Pro Thr Lys Gly Ala Ile Asp Lys Lys 
        115                 120                 125             


Ser Pro Val Ser Gln Arg Ala Lys Lys Ala Gly Gln Lys Leu Gln Lys 
    130                 135                 140                 


Met Gly Val Leu Pro Ile Leu Pro Leu Ser Pro Gly Phe Lys Phe Trp 
145                 150                 155                 160 


Thr Ala Ala Met Met Met Ala Cys Ser Arg Met Asn Ser Trp Glu Ala 
                165                 170                 175     


Cys Asn Glu Lys Thr Ile Glu Asn His Lys Ala Phe Leu Glu Gly Ile 
            180                 185                 190         


Glu Asn Tyr Lys Lys Glu Ile Arg Phe Glu Asp Leu Cys Glu Glu Trp 
        195                 200                 205             


Ser Leu Phe Ser Asp Trp Leu Thr Glu Ala Glu Ser Asp Asn Glu Gly 
    210                 215                 220                 


Gly Cys Lys Phe Lys Leu Thr Pro Arg Phe Leu Gln Arg Trp Glu Arg 
225                 230                 235                 240 


Ile Tyr Leu Lys Gln Met Arg Lys Gly Lys Ile Pro Ala Arg His Asn 
                245                 250                 255     


Leu Gly Pro Val Met Glu Ala Leu Ala Gly Asp Lys Tyr Arg Gln Leu 
            260                 265                 270         


Trp Asp Asn Gly Glu Glu Arg Asp Tyr Ile Thr Glu Leu Gly Asp Leu 
        275                 280                 285             


Val Thr Ser Gln Arg Lys Ala Val Arg Leu Ser Arg Asp Ser Ala Val 
    290                 295                 300                 


Thr Phe Pro Asp Glu Glu Leu Ser Pro Val Gly Thr Glu Phe Gly His 
305                 310                 315                 320 


Asn Tyr Met Ser Phe Ser Ile Asp Gln Glu Asn Ser His Leu Val Thr 
                325                 330                 335     


Leu Glu Val Ile Gly Gly Lys Tyr Gln Phe Glu Ile Ser Lys Ser Asp 
            340                 345                 350         


Tyr Phe Arg Asp Leu Ile Val Glu Glu Ala Gly Lys Gln Ser Lys Phe 
        355                 360                 365             


Tyr Asn Val Ser Tyr Arg Lys Gly Asn Val Arg Glu Glu Asn Leu Ala 
    370                 375                 380                 


Gly Asp Phe Lys Glu Ala Thr Val Arg Asn Arg Arg Ser Leu Lys Thr 
385                 390                 395                 400 


Gly Lys Arg Arg Leu Tyr Phe Tyr Met Ser His Ser Ile Pro Thr Arg 
                405                 410                 415     


Phe Asp Asp Asp Leu Tyr Ala Gln Phe Thr Glu Lys Gly Gln Pro Asp 
            420                 425                 430         


Phe Ser Lys Leu Tyr Lys Ala Val Thr Tyr Phe Gln Cys Ser Leu Gly 
        435                 440                 445             


Asn Lys Lys Ala Asp Thr Tyr Arg Val Tyr Val Lys Met Gly Thr Arg 
    450                 455                 460                 


Phe Leu Gly Val Asp Ile Gly Val Ser Arg Leu Phe Gly Phe Ser Leu 
465                 470                 475                 480 


Phe Glu Leu Arg Glu Glu Lys Pro Glu Lys Asn Pro Phe Phe Glu Leu 
                485                 490                 495     


Pro Asp Asp Leu Gly Tyr Ala Val Cys Leu Glu Ser Trp Val Asp Gly 
            500                 505                 510         


Val Glu Lys Asn His Lys Val Ala Gln Glu Met Lys Asp Trp Arg Arg 
        515                 520                 525             


Glu Cys Leu Ala Ala Gln Arg Leu Ile His Tyr Ala Lys Phe Leu Lys 
    530                 535                 540                 


Lys Arg Asp Lys Asn Glu Glu Ile Asp Tyr Lys His Glu Glu Ser Leu 
545                 550                 555                 560 


Glu Thr Ile Ala Gly Leu Leu Gly Ile Glu Ile Asp Pro Glu Gln Ile 
                565                 570                 575     


Ile Asp Val Pro Leu Lys Leu Leu Asp Leu Val Gly Gln Ala Ile Gly 
            580                 585                 590         


Ala Leu Arg Lys Lys Tyr Leu Val Leu Lys Lys Asn Glu Val Arg Gln 
        595                 600                 605             


Gly Arg Ile Thr Ser Glu Leu Phe Leu Trp Pro Glu Cys Val Asp Thr 
    610                 615                 620                 


Tyr Ile Arg Leu Leu Lys Ser Trp Thr Tyr Lys Asp Lys Lys Pro Tyr 
625                 630                 635                 640 


Gln Lys Gly Glu Thr Asn Lys Asp Ala Phe Lys Lys Leu Lys Gly Tyr 
                645                 650                 655     


Leu Ala Arg Leu Arg Lys Asp Leu Ala Pro Lys Tyr Ala Ala Val Ile 
            660                 665                 670         


Ala Asp Ala Ala Ile Arg His Lys Val His Val Val Val Ala Glu Asn 
        675                 680                 685             


Leu Glu Gln Phe Gly Leu Ser Met Lys Asn Glu Lys Asp Leu Asn Arg 
    690                 695                 700                 


Val Leu Ala His Trp Ser His Gln Lys Ile Trp Ser Met Val Glu Glu 
705                 710                 715                 720 


Gln Leu Arg Pro Tyr Gly Ile Met Val Val Tyr Val Asp Pro Arg His 
                725                 730                 735     


Thr Ser Lys Leu Asp Phe Ala Thr Asp Glu Phe Gly Gly Arg Cys Phe 
            740                 745                 750         


Thr Ser Leu Tyr Val Met Arg Asp Gly Lys Lys Thr Thr Thr Asp Thr 
        755                 760                 765             


Glu Lys Asn Ala Ser Gln Asn Ile Pro Lys Lys Phe Leu Thr Arg His 
    770                 775                 780                 


Arg Asn Val Ser Trp Leu Leu Ala Tyr Ala Val Asp Leu Ser Asp Ser 
785                 790                 795                 800 


Gln Lys Lys Lys Leu Gly Ile Gly Asp Glu Lys Val Trp Leu Pro Asn 
                805                 810                 815     


Met Gly Leu Met Ile Ser Gly Ala Leu Lys Ala Lys His Gly Lys Asn 
            820                 825                 830         


Ser Ala Leu Leu Val Glu Asp Gly Glu Asn Tyr Arg Leu Leu Pro Ile 
        835                 840                 845             


Thr Ala Ala Gln Ala Lys Lys Phe Val Val Lys Arg Lys Lys Glu Glu 
    850                 855                 860                 


Phe Tyr Arg His Gly Glu Ile Trp Leu Thr Lys Glu Ala His Lys Ala 
865                 870                 875                 880 


Arg Ile Glu Tyr Leu Phe Pro Glu Ser Lys Lys Gly Arg Lys Ser 
                885                 890                 895 


<210>  14
<211>  956
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.16İ

<400>  14

Met Lys Lys Thr Asn Tyr Lys Thr Ser His Leu Leu Ile Asp Asn Pro 
1               5                   10                  15      


Pro Gln Ser Ile Ile Asp Leu His Arg Asp Val Ile Glu Ile Gly Ser 
            20                  25                  30          


Tyr Leu Thr Lys Phe Phe Leu Ala Cys Leu Gly Arg Pro Val Asp Ser 
        35                  40                  45              


Thr Ile Leu Ser Glu Pro Ala Leu His Phe Gln Phe Val Asn Gly Ile 
    50                  55                  60                  


Leu Pro Val Lys Asn Gly Pro Gly Ala Asp Asp Ser Ser Trp Arg His 
65                  70                  75                  80  


Ser Glu Asn Cys Tyr Ser Met Leu Phe Glu Lys Asn Ser Lys Ser Gly 
                85                  90                  95      


Lys Ser Asp Gly Lys Val Arg Gln Val Arg Glu Leu Lys Val Ala Leu 
            100                 105                 110         


Phe Gly Lys Lys Glu Lys Gly Lys Gly Ile Val Gly Lys Lys Thr Trp 
        115                 120                 125             


Asp Glu Leu Lys Val Val Leu Glu Ala Leu Pro Glu Glu His Gln Ile 
    130                 135                 140                 


Leu Ser Leu Glu Ile Cys Gln Arg His Tyr Glu Ser Arg Asp Val Lys 
145                 150                 155                 160 


Ala Phe Gly Lys Leu Ala Leu Ser Ser Lys Ser Arg Pro Ser Val Glu 
                165                 170                 175     


Ala Gly Leu Lys Leu Arg Glu Leu Gly Leu Leu Pro Leu Asp Ser Arg 
            180                 185                 190         


Gly Leu Asp Lys Asn Lys Leu Leu Gly Ile Leu Ala Ala Val Thr Gly 
        195                 200                 205             


Arg Leu Lys Ser Trp Arg Asp Arg Asp Cys Ala Cys Lys Ala Asp Lys 
    210                 215                 220                 


Gln Ala Leu Arg Val Lys Phe Glu Glu Arg Leu Ser Lys Val Asp Gln 
225                 230                 235                 240 


Ser Ala Tyr Gln Gln Phe Lys Gln Phe Ala Asp Glu Leu Leu Thr Gln 
                245                 250                 255     


Glu Gly Tyr Arg Ile Ser Gly Arg Val Leu Arg Ala Val Glu Lys Lys 
            260                 265                 270         


Asp Ser Asp Tyr Ser Pro Val Leu Thr Val Leu Ala Lys Tyr Pro Asp 
        275                 280                 285             


Leu Gln Asp Asn Phe Glu Glu Leu Cys Arg Ala Cys Leu Ala Glu Gln 
    290                 295                 300                 


Ala Phe Asn Lys Lys Lys Ala Asp Ala Arg Val Thr Val Cys Ser Glu 
305                 310                 315                 320 


Thr Ser Pro Leu Gln Phe Pro Phe Gly Met Thr Gly Asn Gly Tyr Pro 
                325                 330                 335     


Phe Thr Leu Ser Ala Cys Glu Gly Arg Ile Asn Ala Thr Ile His Phe 
            340                 345                 350         


Pro Gly Gly Asp Leu Pro Leu Arg Leu Arg Lys Ser Lys Tyr Phe Gln 
        355                 360                 365             


Asn Pro Glu Ile Leu Pro Val Lys Asp Gly Phe Gln Ile Thr Phe Thr 
    370                 375                 380                 


Arg Gly Lys Thr Pro Leu Val Gly Thr Ile Lys Glu Pro Ser Leu Leu 
385                 390                 395                 400 


Lys Lys Asn Asn His Tyr Tyr Leu Ser Leu Arg Val Asn Val Pro Ser 
                405                 410                 415     


Val Lys Ile Pro Lys Glu Val Arg Asp Thr Arg Ala Tyr Tyr Ser Ser 
            420                 425                 430         


Ala Val Gly Gly Asp Glu Thr Thr Pro Val Pro Val Lys Ala Val Ala 
        435                 440                 445             


Ile Asp Leu Gly Val Thr Thr Leu Ala Asp Tyr Ser Ile Ile Asp Thr 
    450                 455                 460                 


Cys Leu Pro Gly Asp Cys Lys Val Phe Gly Gly Glu Thr Ala Ala Phe 
465                 470                 475                 480 


Thr Ala His Gly Lys Ile Gly Gln Cys Ala Asn Lys Ser Leu Arg Asp 
                485                 490                 495     


Arg Leu Tyr Lys Asn Thr Glu Glu Ala Leu Phe Leu Gly Lys Phe Ile 
            500                 505                 510         


Arg Leu Ser Lys Lys Leu Arg Asp Gly Glu Gly Leu Asn Arg Trp Glu 
        515                 520                 525             


Val Glu Lys Leu Pro Gly Tyr Ala Glu Arg Leu Gly Ile Thr Gln His 
    530                 535                 540                 


Leu Asp Asn Ala Tyr Thr Arg Lys Asp Glu Ile Ala Arg Lys Phe Lys 
545                 550                 555                 560 


Gln Ile Lys Gly Asn Phe Asp Lys Leu Val Ser Glu Phe Ala Leu Arg 
                565                 570                 575     


Asp His Pro Ser Lys Lys Gly Glu Ser Trp Glu Thr Ile Ser Ala Glu 
            580                 585                 590         


Thr Ile Gln Val Leu Ala Ala Leu Lys Arg Ile Gln Ser Leu Leu Lys 
        595                 600                 605             


Ser Trp Thr Tyr Tyr Ser Trp Thr Ala Glu Asp Tyr Val Leu Ala Leu 
    610                 615                 620                 


Thr Ala Asp Gly Pro Val Cys Ile Asp Gly Glu His Val Lys Ala Val 
625                 630                 635                 640 


Thr Ala Thr Ser Arg Arg Ser Phe Ala Pro Cys Gly Lys Ala Ala Leu 
                645                 650                 655     


Leu Arg Leu Ile Glu Ser Gly Glu Ile Val Glu Thr Gly Gly Gln Tyr 
            660                 665                 670         


Gln Leu Ala Thr Gly Val Lys His Arg Asn His Pro Val Asn Phe Leu 
        675                 680                 685             


Ser Ser Tyr Ile Lys His Phe Asn Gly Leu Arg Arg Asp Leu Thr Asn 
    690                 695                 700                 


Lys Leu Val Arg Ala Ile Val Asn Lys Ala Gln Glu Tyr Arg Val Gln 
705                 710                 715                 720 


Ile Val Ile Val Glu Asp Phe Gly Ile Ala Asp Leu Glu Asp Arg Ile 
                725                 730                 735     


Lys Asp Ala Tyr Glu Asn Tyr Arg Trp Asn Leu Phe Ala Pro Ala Thr 
            740                 745                 750         


Ile Val Lys Lys Leu Glu Ala Ala Leu Leu Glu Val Gly Ile Ala Met 
        755                 760                 765             


Ala Gln Val Asp Pro Arg His Thr Ser Gln Ile Ala Pro Thr Gly Ala 
    770                 775                 780                 


Phe Gly Phe Arg Asp His Ala Phe Leu Tyr Tyr Gln Asp Asp Gly Leu 
785                 790                 795                 800 


Cys Arg Ile Asp Ala Asn Thr Asn Ala Ser Met Arg Ile Ala Glu Arg 
                805                 810                 815     


Phe Phe Met Arg His Ser Val Leu Thr Gln Leu Arg Ala Ala Lys Ile 
            820                 825                 830         


Gly Glu Thr Glu Tyr Leu Ile Pro Glu Ser Ala Ser Lys Arg Leu Asn 
        835                 840                 845             


Ala Phe Val Lys Leu Gln Thr Gly Lys Pro Phe Ala Lys Leu Ile Met 
    850                 855                 860                 


Asn Cys Ser Gly Phe Val Leu Glu Gly Leu Thr Lys Lys Gln Tyr Ala 
865                 870                 875                 880 


Lys Leu Ala Glu Thr Ala Gly Lys Lys Glu Ser Phe Tyr Gln Tyr Asp 
                885                 890                 895     


Asp Arg Trp Phe Asp Lys Gly His His Phe Ala Cys Arg Ala Thr Leu 
            900                 905                 910         


Glu Asn Lys Val Gln Val Cys Leu Asn Gly Gly Gly Arg Ile Lys Asp 
        915                 920                 925             


Thr Thr Pro Asp Phe Asn Pro Lys Ser Leu Leu Arg Ser Asp Leu Gln 
    930                 935                 940                 


Thr Pro Leu Asp Gln Leu Phe Gly Asn Ser Gly Ala 
945                 950                 955     


<210>  15
<211>  946
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.17İ

<400>  15

Met Ser Asn Thr Thr Tyr Lys Thr Ser His Leu Leu Ile Asp Leu Pro 
1               5                   10                  15      


Gln Gln Glu Leu Ile Asp Leu His Arg Asp Ser Asn Glu Met Gly Ser 
            20                  25                  30          


Tyr Leu Thr Lys Phe Phe Leu Ala Ala Leu Gly Arg Pro Val Asp Asn 
        35                  40                  45              


Ser Ile Val Leu Pro Pro Glu Leu Ala Asp Leu Tyr Phe Gln Phe Ala 
    50                  55                  60                  


Asn Gly Ile Leu Pro Val Asp Lys Gly Pro Gly Ser Asp Asp Pro Ser 
65                  70                  75                  80  


Trp Leu His Ser Glu Asn Cys Tyr Ser Met Phe Phe Glu Lys Asp Ser 
                85                  90                  95      


Met Ser Gly Asn Cys Thr Asn Lys Ile Lys Gln Tyr Gln Glu Leu Lys 
            100                 105                 110         


Thr Ala Leu Cys Gly Gln Lys Val Lys Gly Gln Lys Gly Leu Val Gly 
        115                 120                 125             


Lys Lys Thr Trp Ala Gln Leu Lys Lys Val Leu Thr Ala Leu Pro Gln 
    130                 135                 140                 


Lys Tyr Gln Ile Leu Ser Pro Lys Ile Cys Gln Lys Tyr Phe Lys Ser 
145                 150                 155                 160 


Gly Asn Leu Glu Gly Phe Gly Lys Leu Ala Leu Ala Gly Lys Asn Arg 
                165                 170                 175     


Pro Ser Met Ser Ala Gly Leu Gln Leu Arg Glu Leu Gly Leu Leu Pro 
            180                 185                 190         


Leu Asp Ser Arg Gly Ile Asp Lys Asn Lys Leu Leu Gly Ile Leu Val 
        195                 200                 205             


Gly Ile Thr Gly Arg Leu Lys Ser Trp Arg Asp Arg Asp Trp Ala Cys 
    210                 215                 220                 


Lys Thr Val Lys Glu Glu Leu Arg Val Thr Phe Glu Lys Gly Leu Gly 
225                 230                 235                 240 


Glu Val Asp Pro Thr Ala Tyr Pro Gln Phe Lys Gln Phe Ala Asp Gln 
                245                 250                 255     


Leu Phe Lys Gln Glu Gly Tyr Lys Ile Ser Gly Arg Val Leu Arg Ala 
            260                 265                 270         


Val Glu Gly Lys Asp Ala Asp Tyr Gln Pro Val Leu Ser Leu Leu Thr 
        275                 280                 285             


Gln Tyr Pro Asp Leu Gln Gly Asp Phe Glu Glu Leu Gly Arg Val Tyr 
    290                 295                 300                 


Leu Ala Glu Ala Glu Tyr Leu Arg Lys Lys Val Asp Ala Arg Val Thr 
305                 310                 315                 320 


Val Cys Asp Ala Glu Thr Ser Pro Leu Gln Phe Pro Phe Gly Leu Thr 
                325                 330                 335     


Gly Asn Gly Tyr Ser Ile Thr Leu Thr Val Val Lys Gly Gln Ile Ala 
            340                 345                 350         


Ala Thr Leu His Leu Pro Gly Gly Asp Ile Thr Pro Arg Leu Arg Arg 
        355                 360                 365             


Ser Lys Tyr Phe Gln Asn Pro Glu Ile Ala Pro Val Lys Asp Gly Lys 
    370                 375                 380                 


Gly Lys Val Asn Gly Phe Gln Ile Ser Phe Lys Arg Gly Lys Thr Pro 
385                 390                 395                 400 


Leu Val Gly Ile Ile Lys Glu Pro Lys Leu Leu Lys Lys Asn Gly Asn 
                405                 410                 415     


Tyr Tyr Leu Ser Leu Ala Val Gly Ile Asn Lys Thr Glu Ile Pro Lys 
            420                 425                 430         


Glu Ile Cys Asp Ala Arg Ala Tyr Tyr Ser Ser Thr Ser Arg Thr Asp 
        435                 440                 445             


Thr Pro Pro Ala Val Lys Ala Met Ser Ile Asp Leu Gly Val Thr Thr 
    450                 455                 460                 


Leu Ala Asp Tyr Ser Ile Ile Asp Thr Gly Leu Pro Gly Asp Cys Gly 
465                 470                 475                 480 


Val Phe Gly Gly Ser Thr Ala Ala Phe Thr Glu His Gly Lys Ile Gly 
                485                 490                 495     


Arg Cys Gly Ser Lys Ser Leu Arg Asp Gly Leu Tyr Lys Asn Thr Glu 
            500                 505                 510         


Ala Gly Tyr Phe Leu Ala Lys Tyr Ile Arg Leu Ser Lys Asn Leu Arg 
        515                 520                 525             


Gly Gly Val Gly Leu Asn Lys Leu Glu Lys Glu Lys Leu Leu Glu His 
    530                 535                 540                 


Val Glu Arg Leu Gly Ile Glu His Cys Ala Asp Asp Phe Ala Arg Lys 
545                 550                 555                 560 


Asp Glu Ile His Arg Lys Phe Ser Glu Ile Lys Ser Lys Leu Glu Lys 
                565                 570                 575     


Ser Ile Ser Glu Phe Ala Leu Arg Asp Arg Pro Asp Lys Lys Gly Ala 
            580                 585                 590         


Ser Trp Glu Gly Ile Cys Ala Glu Thr Val Gln Val Leu Gly Ala Val 
        595                 600                 605             


Lys Arg Trp Gln Ser Leu Ala Lys Ser Trp Thr Tyr Tyr Ser Trp Thr 
    610                 615                 620                 


Ala Glu Asp Tyr Val Leu Ala Leu Thr Gly Glu Gly Arg Thr Arg Val 
625                 630                 635                 640 


Ser Asp Glu His Val Glu Ser Val Val Lys Thr Gly Arg Arg Gln Phe 
                645                 650                 655     


Ala Pro Cys Gly Lys Ala Ala Leu Leu Arg Leu Leu Glu Lys Gly Lys 
            660                 665                 670         


Ile Val Glu Val Cys Pro Gly Gln Phe Gln Leu Ala Glu Gly Val Asp 
        675                 680                 685             


Tyr Lys Arg His Pro Thr Glu Phe Leu Ala Ala His Ile Arg His Phe 
    690                 695                 700                 


Asn Gly Leu Arg Arg Asp Leu Thr Asn Lys Leu Val Arg Ala Ile Val 
705                 710                 715                 720 


Glu Lys Ala Gln Gln His Arg Val Gln Ile Val Ile Val Glu Asp Phe 
                725                 730                 735     


Gly Ile Pro Asp Ile Glu Gly Arg Ile Met Asp His Tyr Asp Asn Tyr 
            740                 745                 750         


Arg Trp Asn Leu Phe Ala Pro Ala Lys Val Ile Glu Lys Leu Glu Glu 
        755                 760                 765             


Ala Leu Ser Glu Val Gly Ile Ala Met Ala Glu Val Asp Pro Arg His 
    770                 775                 780                 


Thr Ser Gln Leu Ala Pro Thr Gly Asp Phe Gly Phe Arg Asp His Glu 
785                 790                 795                 800 


Asn Leu Tyr Phe Trp Glu Lys Gly Leu Cys Arg Thr Asp Ala Asn Thr 
                805                 810                 815     


Asn Ala Ser Met Arg Ile Ala Glu Arg Phe Phe Thr Arg His Ser Val 
            820                 825                 830         


Leu Ser Gln Leu Arg Ala Val Lys Ile Ser Glu Thr Glu Phe Leu Ile 
        835                 840                 845             


Pro Val Ser Thr Gly Lys Arg Glu Asn Ala Phe Ile Lys Ser Gln Thr 
    850                 855                 860                 


Gly Lys Leu Phe Ala Lys Leu Val Ala Asp Ser Asn Gly Phe Val Met 
865                 870                 875                 880 


Val Gly Leu Thr Glu Lys Gln His Gly Ala Thr Val Thr Val Gly Lys 
                885                 890                 895     


Lys Val Ser Phe Tyr Asn His Ala Gly Arg Trp Leu Gly Lys Ala His 
            900                 905                 910         


His Ile Ala His Arg Asp Arg Ile Lys Asn Glu Val Asn Gln Val Leu 
        915                 920                 925             


Thr Ser Gly Arg Gly Arg Ile Arg Asn Ile Ala Pro Glu Leu Ser Pro 
    930                 935                 940                 


Lys Thr 
945     


<210>  16
<211>  930
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.18İ

<400>  16

Met Thr Asn Gln Lys Pro Lys Phe Lys Ser Ser Asp Ile Gln Ile Lys 
1               5                   10                  15      


His Ile Ser Pro Thr Asp Lys Lys Arg Leu Lys Thr Phe Tyr His Gln 
            20                  25                  30          


Leu Tyr Glu Gln Val Asn Phe Ile Leu Glu Arg Met Ile Val Met Arg 
        35                  40                  45              


Gly Arg Pro Arg Thr Ile Arg Asn Ile Asp Gly Thr Glu Ile Phe Val 
    50                  55                  60                  


Ser Gln Glu Glu Ala Asp Gln Gln Leu Leu Ser Leu Ala Gly Gly Ser 
65                  70                  75                  80  


His Glu Gly Val Lys Tyr Leu Lys Gln Tyr Tyr Glu Ser Cys Val Asp 
                85                  90                  95      


Ala Gly Lys Pro Ala Lys Tyr Ala Ala Asn Met Phe Leu Thr Lys Thr 
            100                 105                 110         


Ile Ser Gly Thr Asn Pro Leu Gln Cys His Thr Ala Val Tyr Lys Leu 
        115                 120                 125             


Tyr Lys Lys Val Gln Ala Lys Gln Ile Thr Lys Lys Glu Phe Ile Asp 
    130                 135                 140                 


Lys Leu Tyr Ser Lys Thr Lys Lys Lys Lys Ser Leu Lys Pro Ala Tyr 
145                 150                 155                 160 


Lys Val Phe Thr Glu Asn Glu His Ile Glu Phe Tyr His Lys Val Arg 
                165                 170                 175     


Ser Gly Lys Leu Pro Ala Ser Glu Val Arg Leu Glu Glu Ser Arg Arg 
            180                 185                 190         


Ala Pro Asp Val Gly Leu Glu Val Gly Leu Leu Leu Arg Glu Leu Gly 
        195                 200                 205             


Ile Phe Pro Phe Asn Phe Pro His Phe Thr Glu Lys Lys Tyr Leu Asp 
    210                 215                 220                 


Leu Ala Trp Thr Ile Ala Ile Arg Trp Leu Lys Asn Trp Asn Glu Asn 
225                 230                 235                 240 


Asn Lys Asn Thr Ala Lys Glu Lys Ala Lys Gln Lys Ala Ile Val Asp 
                245                 250                 255     


Lys Leu Arg Thr Ser Leu Asp Gln Lys Glu Val Asp Leu Phe Glu Glu 
            260                 265                 270         


Phe Ala Glu Glu Cys Ser Gln Glu Gln Phe Gly Ile Arg Glu Gly Phe 
        275                 280                 285             


Val Lys Ala Lys Lys Arg Leu Lys Ser Phe Pro Lys Gly Ile Glu Lys 
    290                 295                 300                 


Ser Ser Tyr Lys Glu Gly Met Arg Ile Leu Val Gln Asn Lys His Gly 
305                 310                 315                 320 


Ser Ile Trp Asp Asn Phe Glu Asn Leu Ala Tyr His His Ile Ala Leu 
                325                 330                 335     


Asn Glu Tyr Asn Arg Leu Arg Asp Glu Ala Ser Phe Ser Phe Pro Asp 
            340                 345                 350         


Pro Ile Tyr His Pro Ile Arg Ala Glu Phe Gly Leu Thr Ser Leu Pro 
        355                 360                 365             


Lys Phe Asn Val Gly Leu Asn Asp Arg Gly Asn Tyr Glu Phe Thr Ile 
    370                 375                 380                 


Asn Leu Pro Asp Gly Pro Leu Met Met Leu Gly Lys Lys Ser Arg Tyr 
385                 390                 395                 400 


Tyr Leu Lys Pro Ile Ile Gln Gly Pro Leu Asn Asn Ala Phe Ser Phe 
                405                 410                 415     


Glu Phe Ile Lys Gly Asn Lys Lys Arg Pro Lys Ile Ser Ala Lys Leu 
            420                 425                 430         


Lys Ser Ile Thr Val Val Phe Ala Lys Ser Ser Ile Tyr Val Gly Leu 
        435                 440                 445             


Pro Tyr Arg Pro Ile Ser Ile Pro Ile Pro Gln Ala Val Thr Asn Ser 
    450                 455                 460                 


Thr Tyr Tyr Phe Lys Lys Asn Leu Ser Ser Thr Ser Lys Phe Asp Lys 
465                 470                 475                 480 


Asp Val Phe Met Gly Leu Thr Ala Val Ser Val Asp Leu Gly Leu Asn 
                485                 490                 495     


Pro Val Phe Ser Met Ser Ala Cys Arg Leu Asp Glu Met Lys Ala Asp 
            500                 505                 510         


Glu His Tyr Ser Cys Glu Val Pro Gly Phe Gly Trp Ala Asn Gln Ile 
        515                 520                 525             


Trp Ser Lys Arg Ala Gly Gly Val Trp Asn Arg Ser Phe Arg Asp Lys 
    530                 535                 540                 


Ile Arg Gly Phe Val Pro Gly Asn Leu Ser Asp Arg Ile Phe Cys Cys 
545                 550                 555                 560 


Lys Lys Ser Ile Ile Val Ser Lys Lys Leu Arg Asp Glu Lys Pro Leu 
                565                 570                 575     


Thr Gln Tyr Glu Glu Glu Asn Phe Glu Arg Trp Met Gln Val Val Gly 
            580                 585                 590         


Val Asp Pro Asn Glu Asp His Tyr Lys Gln Leu Arg Ile Ala Ile Arg 
        595                 600                 605             


Asp Ile Lys Thr Glu Tyr Glu Thr Val Arg Ser Glu Phe Ala Leu Arg 
    610                 615                 620                 


Asp His Pro Asn Asn Ser Asn Lys Thr Thr Glu Asn Ile Cys Thr Glu 
625                 630                 635                 640 


Cys Phe Asp Met Leu Phe Val Ile Lys Asn Leu Ile Ser Leu Leu Lys 
                645                 650                 655     


Ser Trp Asn Arg Trp His Arg Thr Thr Gly Asp Ile Glu Glu Arg Gly 
            660                 665                 670         


Lys Asp Pro Asn Glu Cys Ser Thr Tyr Trp Arg His Tyr Asn Gly Leu 
        675                 680                 685             


Lys Thr Asp Leu Leu Lys Lys Leu Thr Asn Ile Leu Ile Glu Ser Ala 
    690                 695                 700                 


Lys Ser Ile Gly Ala His Ile Ile Ile Leu Glu Asp Leu Thr Leu Ser 
705                 710                 715                 720 


Gln Arg Ser Ser Arg Ser Arg Arg Glu Asn Ser Leu Val Ala Ile Phe 
                725                 730                 735     


Gly Ala Gln Thr Ile Ile Lys Thr Ile Ser Glu Glu Ala Glu Ile Asn 
            740                 745                 750         


Gly Ile Leu Val Tyr Leu Glu Asp Pro Arg His Ser Ser Gln Ile Ser 
        755                 760                 765             


Ile Val Thr Asn Glu Phe Gly Tyr Arg Pro Lys Glu Asp Lys Ala Lys 
    770                 775                 780                 


Leu Tyr Phe Met Asp Glu Glu Thr Val Cys Val Thr Asn Cys Asp Asp 
785                 790                 795                 800 


Ser Ala Ala Leu Met Leu Gln Gln Ser Phe Trp Ser Arg His Lys Asp 
                805                 810                 815     


Val Val Lys Val Lys Gly Thr Lys Val Ser Asp Thr Glu Tyr Leu Val 
            820                 825                 830         


Ser Ser Glu Asp Lys Asp Gly Thr Lys Met Arg Leu Arg Ser Tyr Leu 
        835                 840                 845             


Lys Arg Asn Val Gly Thr Ala Asn Ala Ile Leu Gln Lys Asn Cys Asp 
    850                 855                 860                 


Gly Tyr Asp Leu Lys Lys Ile Ser Pro Gln Lys Lys Lys Lys Ile Glu 
865                 870                 875                 880 


Glu Phe Gly Lys Asp Glu Tyr Phe Tyr Arg His Gly Glu Gln Trp Phe 
                885                 890                 895     


Thr Ala Asp Ala His Phe Asp Lys Leu Arg Glu Phe Gly Asn Gln Val 
            900                 905                 910         


Phe Leu Thr Pro Gln Ser Gln Ile Lys Arg Ile Asn Leu Gln Val Glu 
        915                 920                 925             


Gly Thr 
    930 


<210>  17
<211>  908
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.19İ

<400>  17

Met Pro Ser Tyr Lys Ser Ser Arg Val Leu Val Arg Asp Val Pro Glu 
1               5                   10                  15      


Glu Leu Val Asp His Tyr Glu Arg Ser His Arg Val Ala Ala Phe Phe 
            20                  25                  30          


Met Arg Leu Leu Leu Ala Met Arg Arg Glu Pro Tyr Ser Leu Arg Met 
        35                  40                  45              


Arg Asp Gly Thr Glu Arg Glu Val Asp Leu Asp Glu Thr Asp Asp Phe 
    50                  55                  60                  


Leu Arg Ser Ala Gly Cys Glu Glu Pro Asp Ala Val Ser Asp Asp Leu 
65                  70                  75                  80  


Arg Ser Phe Ala Leu Ala Val Leu His Gln Asp Asn Pro Lys Lys Arg 
                85                  90                  95      


Ala Phe Leu Glu Ser Glu Asn Cys Val Ser Ile Leu Cys Leu Glu Lys 
            100                 105                 110         


Ser Ala Ser Gly Thr Arg Tyr Tyr Lys Arg Pro Gly Tyr Gln Leu Leu 
        115                 120                 125             


Lys Lys Ala Ile Glu Glu Glu Trp Gly Trp Asp Lys Phe Glu Ala Ser 
    130                 135                 140                 


Leu Leu Asp Glu Arg Thr Gly Glu Val Ala Glu Lys Phe Ala Ala Leu 
145                 150                 155                 160 


Ser Met Glu Asp Trp Arg Arg Phe Phe Ala Ala Arg Asp Pro Asp Asp 
                165                 170                 175     


Leu Gly Arg Glu Leu Leu Lys Thr Asp Thr Arg Glu Gly Met Ala Ala 
            180                 185                 190         


Ala Leu Arg Leu Arg Glu Arg Gly Val Phe Pro Val Ser Val Pro Glu 
        195                 200                 205             


His Leu Asp Leu Asp Ser Leu Lys Ala Ala Met Ala Ser Ala Ala Glu 
    210                 215                 220                 


Arg Leu Lys Ser Trp Leu Ala Cys Asn Gln Arg Ala Val Asp Glu Lys 
225                 230                 235                 240 


Ser Glu Leu Arg Lys Arg Phe Glu Glu Ala Leu Asp Gly Val Asp Pro 
                245                 250                 255     


Glu Lys Tyr Ala Leu Phe Glu Lys Phe Ala Ala Glu Leu Gln Gln Ala 
            260                 265                 270         


Asp Tyr Asn Val Thr Lys Lys Leu Val Leu Ala Val Ser Ala Lys Phe 
        275                 280                 285             


Pro Ala Thr Glu Pro Ser Glu Phe Lys Arg Gly Val Glu Ile Leu Lys 
    290                 295                 300                 


Glu Asp Gly Tyr Lys Pro Leu Trp Glu Asp Phe Arg Glu Leu Gly Phe 
305                 310                 315                 320 


Val Tyr Leu Ala Glu Arg Lys Trp Glu Arg Arg Arg Gly Gly Ala Ala 
                325                 330                 335     


Val Thr Leu Cys Asp Ala Asp Asp Ser Pro Ile Lys Val Arg Phe Gly 
            340                 345                 350         


Leu Thr Gly Arg Gly Arg Lys Phe Val Leu Ser Ala Ala Gly Ser Arg 
        355                 360                 365             


Phe Leu Ile Thr Val Lys Leu Pro Cys Gly Asp Val Gly Leu Thr Ala 
    370                 375                 380                 


Val Pro Ser Arg Tyr Phe Trp Asn Pro Ser Val Gly Arg Thr Thr Ser 
385                 390                 395                 400 


Asn Ser Phe Arg Ile Glu Phe Thr Lys Arg Thr Thr Glu Asn Arg Arg 
                405                 410                 415     


Tyr Val Gly Glu Val Lys Glu Ile Gly Leu Val Arg Gln Arg Gly Arg 
            420                 425                 430         


Tyr Tyr Phe Phe Ile Asp Tyr Asn Phe Asp Pro Glu Glu Val Ser Asp 
        435                 440                 445             


Glu Thr Lys Val Gly Arg Ala Phe Phe Arg Ala Pro Leu Asn Glu Ser 
    450                 455                 460                 


Arg Pro Lys Pro Lys Asp Lys Leu Thr Val Met Gly Ile Asp Leu Gly 
465                 470                 475                 480 


Ile Asn Pro Ala Phe Ala Phe Ala Val Cys Thr Leu Gly Glu Cys Gln 
                485                 490                 495     


Asp Gly Ile Arg Ser Pro Val Ala Lys Met Glu Asp Val Ser Phe Asp 
            500                 505                 510         


Ser Thr Gly Leu Arg Gly Gly Ile Gly Ser Gln Lys Leu His Arg Glu 
        515                 520                 525             


Met His Asn Leu Ser Asp Arg Cys Phe Tyr Gly Ala Arg Tyr Ile Arg 
    530                 535                 540                 


Leu Ser Lys Lys Leu Arg Asp Arg Gly Ala Leu Asn Asp Ile Glu Ala 
545                 550                 555                 560 


Arg Leu Leu Glu Glu Lys Tyr Ile Pro Gly Phe Arg Ile Val His Ile 
                565                 570                 575     


Glu Asp Ala Asp Glu Arg Arg Arg Thr Val Gly Arg Thr Val Lys Glu 
            580                 585                 590         


Ile Lys Gln Glu Tyr Lys Arg Ile Arg His Gln Phe Tyr Leu Arg Tyr 
        595                 600                 605             


His Thr Ser Lys Arg Asp Arg Thr Glu Leu Ile Ser Ala Glu Tyr Phe 
    610                 615                 620                 


Arg Met Leu Phe Leu Val Lys Asn Leu Arg Asn Leu Leu Lys Ser Trp 
625                 630                 635                 640 


Asn Arg Tyr His Trp Thr Thr Gly Asp Arg Glu Arg Arg Gly Gly Asn 
                645                 650                 655     


Pro Asp Glu Leu Lys Ser Tyr Val Arg Tyr Tyr Asn Asn Leu Arg Met 
            660                 665                 670         


Asp Thr Leu Lys Lys Leu Thr Cys Ala Ile Val Arg Thr Ala Lys Glu 
        675                 680                 685             


His Gly Ala Thr Leu Val Ala Met Glu Asn Ile Gln Arg Val Asp Arg 
    690                 695                 700                 


Asp Asp Glu Val Lys Arg Arg Lys Glu Asn Ser Leu Leu Ser Leu Trp 
705                 710                 715                 720 


Ala Pro Gly Met Val Leu Glu Arg Val Glu Gln Glu Leu Lys Asn Glu 
                725                 730                 735     


Gly Ile Leu Ala Trp Glu Val Asp Pro Arg His Thr Ser Gln Thr Ser 
            740                 745                 750         


Cys Ile Thr Asp Glu Phe Gly Tyr Arg Ser Leu Val Ala Lys Asp Thr 
        755                 760                 765             


Phe Tyr Phe Glu Gln Asp Arg Lys Ile His Arg Ile Asp Ala Asp Val 
    770                 775                 780                 


Asn Ala Ala Ile Asn Ile Ala Arg Arg Phe Leu Thr Arg Tyr Arg Ser 
785                 790                 795                 800 


Leu Thr Gln Leu Trp Ala Ser Leu Leu Asp Asp Gly Arg Tyr Leu Val 
                805                 810                 815     


Asn Val Thr Arg Gln His Glu Arg Ala Tyr Leu Glu Leu Gln Thr Gly 
            820                 825                 830         


Ala Pro Ala Ala Thr Leu Asn Pro Thr Ala Glu Ala Ser Tyr Glu Leu 
        835                 840                 845             


Val Gly Leu Ser Pro Glu Glu Glu Glu Leu Ala Gln Thr Arg Ile Lys 
    850                 855                 860                 


Arg Lys Lys Arg Glu Pro Phe Tyr Arg His Glu Gly Val Trp Leu Thr 
865                 870                 875                 880 


Arg Glu Lys His Arg Glu Gln Val His Glu Leu Arg Asn Gln Val Leu 
                885                 890                 895     


Ala Leu Gly Asn Ala Lys Ile Pro Glu Ile Arg Thr 
            900                 905             


<210>  18
<211>  821
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.20İ

<400>  18

Met Ala Phe Gln Ser Lys Arg Arg Ile Val Gly Asn Phe Val Lys Glu 
1               5                   10                  15      


Gln Cys Leu Lys Ala Val Asp Gly Lys Val Ile Leu Thr Asp Gln Glu 
            20                  25                  30          


Lys Arg Glu Leu Ile Lys Arg Tyr Glu Leu His Leu Glu Pro His Lys 
        35                  40                  45              


Trp Leu Leu Arg Leu Phe Leu Ser Gly Tyr Glu Gly Arg Asp Asp Gly 
    50                  55                  60                  


Phe Tyr Glu Glu Leu Gly Asn Thr Asn Leu Asp Lys Glu Lys Phe Phe 
65                  70                  75                  80  


Glu Val Thr Ala Gly Leu Arg Asp Ala Leu Leu Arg Gln Ser Gly Ser 
                85                  90                  95      


Ser Arg Ala Leu Lys Ser Ser Met Leu Gly Lys Cys Pro Pro Ser Ala 
            100                 105                 110         


Ala Val Gly Lys Ala Ala Lys His Ile Gln Thr Leu Arg Asp Ala Gly 
        115                 120                 125             


Ile Leu Pro Phe Lys Thr Gly Leu Thr Ser Gly Glu Asp Tyr Asn Val 
    130                 135                 140                 


Leu Gln Gln Ala Val Gln Gln Leu Arg Ser Trp Val Ala Cys Asp His 
145                 150                 155                 160 


Arg Thr Arg Glu Ala Tyr Ala Glu Gln Gln Glu Lys Thr Ser Gln Ala 
                165                 170                 175     


Glu Glu Ala Ala Lys Lys Ala Ala Asn Glu Val Lys Pro Glu Asp Ala 
            180                 185                 190         


Lys Ser Leu Glu Arg His Glu Arg Val Leu Thr Lys Leu Arg Lys Gln 
        195                 200                 205             


Glu Arg Arg Leu Glu Arg Met Lys Ser His Ala Gln Phe Ser Leu Asp 
    210                 215                 220                 


Glu Met Asp Cys Thr Gly Tyr Ser Leu Cys Met Gly Ala Asn Tyr Leu 
225                 230                 235                 240 


Lys Asp Tyr Cys Leu Glu Lys Glu Gly Arg Gly Leu Arg Leu Thr Leu 
                245                 250                 255     


Lys Asn Ser Thr Met Ala Gly Ser Tyr Tyr Val Ser Val Gly Asp Gly 
            260                 265                 270         


Gln His Ala Gly Met Lys Asn Pro Gly Thr Pro Ala Gly Gly Ser Pro 
        275                 280                 285             


Glu Lys Gly Arg Arg Arg Asn Ile Leu Phe Asp Phe Thr Val Glu Lys 
    290                 295                 300                 


Cys Gly Asp Asn Tyr Leu Phe Arg Tyr Asp Glu Asn Gly Lys Arg Pro 
305                 310                 315                 320 


Arg Ala Gly Val Val Lys Glu Pro Arg Phe Cys Trp Arg Arg Lys Gly 
                325                 330                 335     


Asn Ser Val Glu Leu Tyr Leu Ala Met Pro Ile Asn Ile Glu Asn Ser 
            340                 345                 350         


Met Arg Asn Ile Phe Val Gly Lys Gln Lys Ser Gly Lys His Ser Ala 
        355                 360                 365             


Phe Thr Arg Gln Trp Pro Lys Glu Val Glu Gly Leu Asp Glu Leu Arg 
    370                 375                 380                 


Asp Ala Val Val Leu Gly Val Asp Ile Gly Ile Asn Arg Ala Ala Phe 
385                 390                 395                 400 


Cys Ala Ala Leu Lys Thr Ser Arg Phe Glu Asn Gly Leu Pro Ala Asp 
                405                 410                 415     


Val Gln Val Met Asp Thr Thr Cys Asp Ala Leu Thr Glu Lys Gly Gln 
            420                 425                 430         


Glu Tyr Arg Gln Leu Arg Lys Asp Ala Thr Cys Leu Ala Trp Leu Ile 
        435                 440                 445             


Arg Thr Thr Arg Arg Phe Lys Ala Asp Pro Gly Asn Lys His Asn Gln 
    450                 455                 460                 


Ile Lys Glu Lys Asp Val Glu Arg Phe Asp Ser Ala Asp Gly Ala Tyr 
465                 470                 475                 480 


Arg Arg Tyr Met Asp Ala Ile Ala Glu Met Pro Ser Asp Pro Leu Gln 
                485                 490                 495     


Val Trp Glu Ala Ala Arg Ile Thr Gly Tyr Gly Glu Trp Ala Lys Glu 
            500                 505                 510         


Ile Phe Ala Arg Phe Asn His Tyr Lys His Glu His Ala Cys Cys Ala 
        515                 520                 525             


Val Ser Leu Ser Leu Ser Asp Arg Leu Val Trp Cys Arg Leu Ile Asp 
    530                 535                 540                 


Arg Ile Leu Ser Leu Lys Lys Cys Leu His Phe Gly Gly Tyr Glu Ser 
545                 550                 555                 560 


Lys His Arg Lys Gly Phe Cys Lys Ser Leu Tyr Arg Leu Arg His Asn 
                565                 570                 575     


Ala Arg Asn Asp Val Arg Lys Lys Leu Ala Arg Phe Ile Val Asp Ala 
            580                 585                 590         


Ala Val Asp Ala Gly Ala Ser Val Ile Ala Met Glu Lys Leu Pro Ser 
        595                 600                 605             


Ser Gly Gly Lys Gln Ser Lys Asp Asp Asn Arg Ile Trp Asp Leu Met 
    610                 615                 620                 


Ala Pro Asn Thr Leu Ala Thr Thr Val Cys Leu Met Ala Lys Val Glu 
625                 630                 635                 640 


Gly Ile Gly Phe Val Gln Val Asp Pro Glu Phe Thr Ser Gln Trp Val 
                645                 650                 655     


Phe Glu Gln Arg Val Ile Gly Asp Arg Glu Gly Arg Ile Val Ser Cys 
            660                 665                 670         


Leu Asp Ala Glu Gly Val Arg Arg Asp Tyr Asp Ala Asp Glu Asn Ala 
        675                 680                 685             


Ala Lys Asn Ile Ala Trp Leu Ala Leu Thr Arg Glu Ala Glu Pro Phe 
    690                 695                 700                 


Cys Met Ala Phe Glu Lys Arg Asn Gly Val Val Glu Pro Lys Gly Leu 
705                 710                 715                 720 


Arg Phe Asp Ile Pro Glu Glu Pro Thr Arg Glu Gln Asp Glu Ser Asp 
                725                 730                 735     


Gln Asp Phe Lys Lys Arg Leu Glu Glu Arg Asp Lys Leu Ile Glu Arg 
            740                 745                 750         


Leu Gln Ala Lys Ala Asp Arg Met Gln Ala Ile Val Gln Arg Leu Phe 
        755                 760                 765             


Gly Asp Arg Arg Pro Trp Asp Ala Phe Ala Asp Arg Ile Pro Glu Gly 
    770                 775                 780                 


Lys Ser Lys Arg Leu Phe Arg His Arg Asp Gly Leu Val Leu Asn Lys 
785                 790                 795                 800 


Pro Phe Lys Gly Leu Cys Gly Ser Glu Asn Ser Glu Gln Lys Ala Ser 
                805                 810                 815     


Ala Arg Asn Ser Arg 
            820     


<210>  19
<211>  837
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.21İ

<400>  19

Met Gly Arg Phe Gly Lys Lys Lys Ile Ala Val Asn Gly Tyr Val Glu 
1               5                   10                  15      


Gln Asp Cys Ile Lys Thr Ile Ser Ala Lys Cys Leu Leu Thr Arg Ala 
            20                  25                  30          


Gln Ile Asp Glu Leu Arg Ala Lys Tyr Asp Ala Val Leu Asp Thr Met 
        35                  40                  45              


Arg Pro Leu Ile Arg Leu Ile Leu Ala Gly Tyr Glu Gly Arg Asp Asp 
    50                  55                  60                  


Gly Ile Tyr Glu Glu Ile Ala Pro Glu Met Ser Lys Lys Lys Phe Phe 
65                  70                  75                  80  


Glu Ala Ala Thr Glu Trp Arg Glu Ser Ile Val Lys Asn Ala Ser Pro 
                85                  90                  95      


Arg Ala Met Lys Ala Ser Val Phe Gly Asp Lys Glu Pro Cys Lys Ser 
            100                 105                 110         


Thr Gly Gly Ala Arg Ala Val Ile Gly Lys Leu Arg Lys Ser Gly Val 
        115                 120                 125             


Phe Pro Ile Glu Thr Gly Leu Ser Gly Gly Asp Glu Tyr Asn Leu Ile 
    130                 135                 140                 


Glu Gln Ala Ile Glu Tyr Ala Lys Ser Trp Leu Lys Ser Asp Glu Ala 
145                 150                 155                 160 


Thr Arg Glu Ala Tyr Ala Asp Gln Gln Lys Asp Ile Lys Arg Leu Ile 
                165                 170                 175     


Gly Glu Ala Lys Lys Leu Ala Leu Lys Ile Glu Lys Ala Glu Lys Lys 
            180                 185                 190         


Leu Glu Ala Thr Asn Pro Gln Thr Lys Ser Trp Lys Lys Thr Thr Glu 
        195                 200                 205             


Ile Ile Lys Lys Ser Lys Arg Glu Phe Gly Ser Val Thr Thr Lys Thr 
    210                 215                 220                 


Glu Lys Ala Glu Lys Arg Phe Glu Arg Met Lys Pro Phe Ser Lys Leu 
225                 230                 235                 240 


Glu Leu Gln Asn Met Asp Cys Thr Lys Tyr Ser Thr Tyr Leu Gly Thr 
                245                 250                 255     


Asn Tyr Ser Pro Phe Lys Leu Lys Lys Glu Gly Asp Leu Leu Gln Ile 
            260                 265                 270         


Thr Val Thr Ser Ser Val Met Lys Gly Thr Tyr Leu Ala Ser Tyr Gly 
        275                 280                 285             


Asp Gly Gln Tyr Gly Ser Arg Arg Asn Asn Gly Gln Ser Arg Arg Asp 
    290                 295                 300                 


Asp Phe Val Pro Asn Met Asn Gln Lys Arg Arg Arg Asn Leu Met Phe 
305                 310                 315                 320 


Asp Cys Thr Val Glu Pro Phe Gly Asp Gly Ser Leu Leu Arg Tyr Glu 
                325                 330                 335     


Glu Asn Gly Leu Arg Pro Arg Val Ala Glu Leu Lys Glu Pro Arg Leu 
            340                 345                 350         


Cys Trp Arg Arg Arg Asn Gly Asn Tyr Glu Leu Tyr Leu Met Met Pro 
        355                 360                 365             


Val Lys Met His Val Lys Ser Pro Glu Met Phe Ala Gly Asp His Leu 
    370                 375                 380                 


Ala Phe Ser Arg Tyr Trp Pro Lys Glu Val Glu Gly Leu Asp Ser Asp 
385                 390                 395                 400 


Thr Lys Ile Thr Ala Leu Gly Val Asp Val Gly Ile Ile Arg Ser Ala 
                405                 410                 415     


Tyr Cys Val Ala Val Thr Ala Glu Arg Phe Val Asp Gly Leu Pro Thr 
            420                 425                 430         


Glu Met Thr Val Gly Lys Ala Ser Phe Asp Ala Gln Thr Glu Lys Gly 
        435                 440                 445             


Arg Glu Tyr Phe Glu Leu Gly Arg Arg Ala Thr Met Leu Gly Trp Leu 
    450                 455                 460                 


Ile Lys Thr Thr Arg Arg Tyr Lys Lys Asp Pro Lys Asn Glu His Asn 
465                 470                 475                 480 


Gln Ile Lys Glu Ser Asp Val Ala Ala Phe Asp Gly Ser Pro Gly Ala 
                485                 490                 495     


Phe Glu His Tyr Ile Leu Ala Val Asp Glu Met Ser Asp Asp Pro Leu 
            500                 505                 510         


Asp Val Trp Gly His Ala Asn Ile Thr Gly Tyr Gly Lys Trp Thr Lys 
        515                 520                 525             


Gln Ile Phe Lys Glu Phe Asn Gln Leu Lys Arg Glu Arg Ala Glu Gly 
    530                 535                 540                 


Gln Val Glu Pro Asn Met Thr Asp Asp Leu Thr Trp Cys Ser Leu Ile 
545                 550                 555                 560 


Asp Tyr Ile Ile Ser Leu Lys Lys Thr Leu His Phe Gly Gly Tyr Glu 
                565                 570                 575     


Thr Lys Glu Arg Glu Ser Phe Cys Pro Ala Leu Tyr Asn Glu Arg Ala 
            580                 585                 590         


Asn Cys Arg Asp Val Val Arg Lys Arg Leu Ala Arg Tyr Val Val Glu 
        595                 600                 605             


Arg Ala Ile Ala Ala Glu Ala Gln Val Ile Ser Val Glu Asn Leu Ser 
    610                 615                 620                 


Lys Cys Arg Arg Asp Asp Lys Arg Lys Asn Arg Val Trp Asp Leu Met 
625                 630                 635                 640 


Ser Gln Gln Ser Trp Ile Gly Val Leu Thr Asn Met Ala Arg Met Glu 
                645                 650                 655     


Asn Ile Ala Val Val Ser Val Asn Pro Asp Leu Thr Ser Gln Trp Val 
            660                 665                 670         


Glu Gln Cys Gly Ala Ile Gly Asp Arg Lys Ala Arg Thr Ile Ala Cys 
        675                 680                 685             


Arg Asp Val Asn Gly Lys Phe Val Ser Leu Asp Ala Asp Leu Asn Ala 
    690                 695                 700                 


Ala Tyr Asn Ile Ala Ser Arg Ala Leu Thr Arg His Ala Glu Pro Phe 
705                 710                 715                 720 


Ser Ile Thr Phe Lys Lys Lys Asp Gly Ile Leu Glu Gln Lys Asp Val 
                725                 730                 735     


Cys Phe Asp Pro Gly Val Ile Pro Val Leu Glu Lys Asn Glu Asn Glu 
            740                 745                 750         


Glu Lys Phe Arg Glu Arg Val Glu Lys Tyr Glu Lys Ser Leu Val Ile 
        755                 760                 765             


Lys Gln Glu Arg Ala Val Arg Trp Arg Ala Ile Leu Gln His Leu Phe 
    770                 775                 780                 


Gly Asn Glu Arg Pro Trp Asp Glu Phe Thr Asp Glu Val Lys Glu Gly 
785                 790                 795                 800 


Arg His Val Ser Leu Tyr Arg His His Gly Lys Leu Val Arg Thr Lys 
                805                 810                 815     


Gln Tyr Ala Gly Leu Val Lys Glu Ala Asn Asn Glu Leu Val Pro Val 
            820                 825                 830         


Cys Ala Val Ala Arg 
        835         


<210>  20
<211>  968
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.22İ

<400>  20

Met Ser Lys Ala Thr Arg Lys Thr Lys Thr Thr Val Pro Glu Ser Thr 
1               5                   10                  15      


Asp Thr Glu Ser Pro Ala Ala Asp Thr Gln Val Arg Val His Trp Leu 
            20                  25                  30          


Ala Ala Ser His Arg Ala Ser Pro Gly Leu Gln Gln Val Lys Glu Met 
        35                  40                  45              


Ile Gln Gln His Ala Asp Val Ala Ser Val Leu Phe Gln Gly Leu Val 
    50                  55                  60                  


Arg Thr Ala Pro Ile Val Phe Arg Asn Asp Asp Gly Ser Pro Val Lys 
65                  70                  75                  80  


Pro Leu Asp Leu Leu Leu Ala Ser Leu Arg Pro Thr Tyr Lys Val Gln 
                85                  90                  95      


Arg Asp Thr Glu Thr Val Leu Val Thr Lys Asp Asp Val Ile Arg Cys 
            100                 105                 110         


Leu Thr Leu Ala Thr Thr Ala Val Asn Gly Gly Gln Ala Thr Asn Val 
        115                 120                 125             


Ala Val Phe Ala Ser Ala Asp Pro Ala Leu Ser Ala Pro Leu Ala Thr 
    130                 135                 140                 


Leu Leu Ala Gln Leu Arg Ala Leu Glu Ser Val Asp Ser Ser Trp Ser 
145                 150                 155                 160 


Val Val Gly Lys Leu Asp Ile Asn Leu Arg Lys Phe Val Trp Leu Val 
                165                 170                 175     


Leu Ser Ala Ala Gly Val Leu Pro Ala Leu Ala Asp Leu Glu Gly Tyr 
            180                 185                 190         


Ala Ala Lys Ser Val Leu Ala Asn Val Gln Gly Lys Tyr Lys Ser Leu 
        195                 200                 205             


Gln Ala Cys Ala Asp Thr His Ala Ala Leu Tyr Lys Gln His Gln Thr 
    210                 215                 220                 


Asn Lys Glu Gln Leu Glu Lys Leu Ile Ala Asp Pro Gly Phe Val Ala 
225                 230                 235                 240 


Leu Cys Ser Ala Leu Leu Gln Asp Pro Asp Leu Arg Ser Val Asp Ser 
                245                 250                 255     


Arg Arg Leu Ala Ala Leu Glu Glu Met Leu Gly Phe Val Ala Ala Asp 
            260                 265                 270         


Lys Asn Tyr Ser Glu Tyr Thr Ser Thr Arg Lys Cys Asp Gly Trp Ala 
        275                 280                 285             


Pro Pro Ala Asn Met Phe Asp Leu Leu Cys Glu His Lys Glu Ala Val 
    290                 295                 300                 


Arg Arg Asn Ile Val Val Asp Asn Ser Lys Cys Leu Ser Arg Arg Ile 
305                 310                 315                 320 


Ser Leu Val Ala Asp Gly Asp Val Asn Glu Val Ser Val Phe Glu Leu 
                325                 330                 335     


Leu Asn Glu Met Arg Trp Leu Ser Val His Ser Ser Gly Ile Arg Met 
            340                 345                 350         


Pro Asn Tyr Pro Lys His Ala Tyr Ala Leu Lys Phe Gly Asp Asn Tyr 
        355                 360                 365             


Ile Ser Val Lys Ser Phe Glu Thr Val Val Asp Gly Gly Cys Ser Leu 
    370                 375                 380                 


Leu Arg Met Thr Ala Arg Val Gly Lys Asn Asp Leu Val Cys Asp Phe 
385                 390                 395                 400 


Val Leu Gly Arg Gly Asn Glu Tyr Trp Asn Asn Leu Lys Ile Thr Pro 
                405                 410                 415     


Met Gly Lys Gly Ile Phe Ala Val Val Lys Thr Val Arg Arg Phe Thr 
            420                 425                 430         


Ala Thr Gly Ala Lys Leu Val Glu Leu Arg Gly Val Cys Lys Glu Pro 
        435                 440                 445             


Glu Ile Arg Tyr Glu Arg Gly Val Leu Gly Leu Arg Leu Pro Ile Ser 
    450                 455                 460                 


Phe Asp Val Tyr Gly Lys Val Glu Glu Asp Ser Ile Ala Phe Gly Lys 
465                 470                 475                 480 


Asn Arg Val Ser Leu Arg Thr Thr Pro Phe Val Glu Lys Ala Asp Lys 
                485                 490                 495     


Phe Gln Gly Leu Leu Asp Tyr Arg Asn Thr Thr Ala Arg Asp Gly Tyr 
            500                 505                 510         


Ile Tyr Tyr Ala Gly Phe Asp Gln Gly Glu Asn Asp Gln Val Val Gly 
        515                 520                 525             


Ile Tyr Arg Thr Arg Thr Tyr Lys Asn Ala Thr Met Leu Glu Phe Phe 
    530                 535                 540                 


Asn Val Ser Asp Thr Leu Glu Glu Val Ala Ser Cys Arg Phe Ser Asp 
545                 550                 555                 560 


Tyr Gln Glu Arg Lys Arg Arg Leu Arg Gly Asp Thr Gly Val Leu Asp 
                565                 570                 575     


Ile Asn Ser Ile Asn Val Leu Ala Asp Lys Val Gln Arg Leu Arg Arg 
            580                 585                 590         


Leu Ile Ser Thr Leu Arg Ala Cys Ala Ser His Thr Asp Trp Tyr Pro 
        595                 600                 605             


Lys Leu Lys Glu Arg Arg Arg Leu Glu Trp Ala Val Leu Ala Gln Gly 
    610                 615                 620                 


Val Gly Val Ser Asp Phe Asp Thr Glu Ile Glu Arg Ala Glu Thr Ala 
625                 630                 635                 640 


Leu Ser Ala Val Ala Ala Val Asp Phe Val Arg Asp Pro Thr Cys Ile 
                645                 650                 655     


Ile Asn Val Met Asp Lys His Ile Tyr Ala Gln Phe Lys Gln Leu Arg 
            660                 665                 670         


Ser Glu Arg Asn Glu Lys Tyr Arg Ser Gln His Gln His Asp Tyr Lys 
        675                 680                 685             


Trp Leu Gln Leu Val Asp Ser Val Ile Ser Leu Arg Lys Ser Ile Tyr 
    690                 695                 700                 


Arg Phe Gly Lys Ala Pro Glu Pro Arg Gly Ala Gly Glu Leu Tyr Pro 
705                 710                 715                 720 


Gln Asn Leu Tyr Thr Tyr Arg Asp Asn Leu Met Gln Gln Tyr Arg Lys 
                725                 730                 735     


Glu Val Ala Ala Phe Ile Arg Asp Val Cys Leu Glu His Gly Val Arg 
            740                 745                 750         


Gln Leu Ala Val Glu Ala Leu Asn Pro Thr Ser Tyr Ile Gly Glu Asp 
        755                 760                 765             


Ser Asp Ala Asn Arg Lys Arg Ala Leu Phe Ala Pro Ser Glu Leu His 
    770                 775                 780                 


Asn Asp Ile Val Leu Ala Cys Ser Leu His Ser Ile Ala Val Val Ala 
785                 790                 795                 800 


Val Asp Glu Thr Met Thr Ser Arg Val Ala Pro Asn Asn Arg Leu Gly 
                805                 810                 815     


Phe Arg Ser His Gly Asp Tyr Gln Lys Phe Ser Glu Thr Ala Gln Gly 
            820                 825                 830         


Arg Phe Asn Trp Lys His Leu His Tyr Phe Gly Asp Asn Asp Val Ser 
        835                 840                 845             


Glu His Cys Asp Ala Asp Glu Asn Ala Cys Arg Asn Ile Val Leu Arg 
    850                 855                 860                 


Ala Leu Thr Cys Gly Ala Ser Lys Pro Arg Phe Ser Arg Gln Ser Leu 
865                 870                 875                 880 


Leu Gly Lys Ile Lys Gly Pro Val Leu Arg Thr Gln Leu Ala Tyr Leu 
                885                 890                 895     


Ala His Lys Arg Gly Leu Leu Thr Ala Ser Thr Glu Pro Lys Lys Ala 
            900                 905                 910         


Ala Glu Thr Gly Phe Glu Leu Val Glu Ala Asp Leu Gly Gly Ala Leu 
        915                 920                 925             


Arg Val Gly Lys Gly Phe Ile Tyr Val Asp Ala Gly Ile Cys Ile Asn 
    930                 935                 940                 


Ala Thr Thr Arg Lys Glu Arg Ser His Lys Val Gly Glu Ala Val Val 
945                 950                 955                 960 


Ser Arg Ser Leu Ala Ser Pro Phe 
                965             


<210>  21
<211>  3012
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.3

<400>  21
atgaccaagg agaagatcaa gaagaccaag aaggccaagg tggagaagga ctccgtgacc       60

agggccggca tcctgaggat cctgctgaac ccggaccagc accaggagct ggacaccctg      120

atctccgacc accaggaggc cgccagggag atccagaccg ccacctacaa gctgtccggc      180

ctgaagctgt acgacaagac caacaacatg gtggtggacg gctccaaggc caccccggag      240

gagcaggagg cctactacaa gatcatcaac tgggagggcc agccgatctc catctccaac      300

ccgatggtga gggccacctt caagtccatc gccaaggtga aggaggacat caggaggaag      360

caggaggagt acgccaagct ggaggaggcc gacctgacca agatgtccac cggcgacgtg      420

aagaagcaca agaacgagct gaggaaggcc gccaacagga tcaagcactc cgaggagatc      480

ctgcagttcg ccaagtggag gctggccgac atcttcccgc tgccgctgtc ccacaactcc      540

cagctgcacc tgaagaacaa ctaccaccag aacgtgttct ccggcttcca cgccagggtg      600

aagggctgga acgcctgcga catcgccgcc caggccaact acgccgagat cgacaacagg      660

ctgaccgagc tgtcctccga gctgtccggc gactacggct ccgaggtgat caccgacctg      720

atgggcctgc tgcagtacac caaggagctg ggcgagggct acaccgacac ctcctacctg      780

aactacaagt tcctgtcctt cttcaaggag tgctggaggc cgaacgccat cgccaacaac      840

accggcctgc tggagggctt ctggctggcc aacaacaagc acaccaacaa gaagaaccag      900

gtggcctact ccttcaaccc gaagatctcc gaggagctgt tcaggaggag gtccctgtgg      960

gagtccgaca agtgcctgct gtccgacccg aggttcgaga agtacgtgga gctgttcgac     1020

aagcacggca ggtacaggaa gggcgcctcc ctgaccctga tctccaagga gtccccgatc     1080

ccgatcggct tctccatgga caggaacgcc gccaagctgg tgaggatcga caacgacacc     1140

gccaacaggc agctgaccat caccatcgag ctgccgaaca aggaggagag gtcctacgtg     1200

gccgcctacg gcaggaagca cgagaccaag tgctactaca acggcctgac caccaggctg     1260

ccgaggtccg agaaggagct gctggccctg gccaaggccg agaacaggga gctgaccgac     1320

aaggagatcc acgaggcctc cctggagaag tgctacatct tcgagtacgc cagggccggc     1380

aagatcccgg tgttcgccgt ggtgaagacc ctgtacttca ggaggaaccc gtccaacggc     1440

gagtactacg tgatcctgcc gaccaacatc ttcgtggagt accacgccaa caacgagttc     1500

aactccaagg agctgttcaa gatcaggtcc gagctgcaga aggcctggga cgaggtgagg     1560

accccgaaga ggaacgtgca gtcctgcgtg ctggacaagg acctgtccaa gaggttcgcc     1620

ggcaggaccc tgaagtacgc cggcatcgac ctgggctact ccaacccgta caccgtgtcc     1680

tactacaacg tggtgggcac cgaggagggc atccagatca aggagaccgg caacgagatc     1740

gtgtccaccg tgttcaacga gcagtacatc cagctgaagg gcaacatcta ccagctgatc     1800

aacatcatca gggcctccag gaggtacctg caggagtccg gcgagctgaa gctgtccaag     1860

gacgacatca agtccttcga ccagctgatg gagctgctgc cgtccgagca gaggatcacc     1920

atcgaccagt tcatcaagga catcaagaag gccaagcagg agggcaagct gatcagggac     1980

atcaagggca agctgccggt ggagggcaag aagaaggagt actgggtgat ctccaacctg     2040

atgtacgtga tcacccagac catgaacggc atcaggggca acagggactc caacaaccac     2100

ctgaccgaga agaagaactg gctgtccgcc ccgccgctga tcgagctgat cgacgcctac     2160

tacaacctga agaagacctt caacgactcc ggcgacggca tcaagatgct gccgaaggac     2220

cacgtgtacg ccgagggcga gaagcagagg tgcaccctga gggaggagaa cttctgcaag     2280

ggcatcctgg agtggaggga caacgtgaag gactacttca tcaagaagct gttctcccag     2340

atcgcccaca ggtgctacga gctgggcatc ggcatcgtgg ccatggagaa cctggacatc     2400

atgggctcct ccaagaacac caagcagtcc aacaggatgt tcaacatctg gccgaggggc     2460

cagatgaaga agtccgccga ggacgccttc tcctacatgg gcatcctgat ccagtacgtg     2520

gacgagaacg gcacctccag gcacgacgcc gactccggca tctacggctg cagggacggc     2580

gccaacctgt ggctgccgaa caagaagctg cacgccgacg tgaacgcctc caggatgatc     2640

gccctgaggg gcctgaccca ccacaccaac ctgtactgca ggtccctgac cgagatcgag     2700

aacggcaagt acgtgaacac ctacgagctg ttcgacacca ccaagaacga ccagtccggc     2760

gccgccaaga ggctgagggg cgccgagacc ctgctgcacg gctactccgc caccgtgtac     2820

cagatccaca ccaccaacac cggcgccggc gtggccctgc tgccggacct gaccgccacc     2880

gacgtgatca agaacaagaa gatcaccgcc accaaggaga acaccgccaa gtactacaag     2940

ctggacaaca ccaacaccta ctacccgtgg tccgtgtgcg agaagctgca caagaactgg     3000

aagctgtcct ga                                                         3012


<210>  22
<211>  2625
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.4

<400>  22
atgaagaaga agaagaactt ctccgtgtcc gccaccggcg tgttctcctt cccgaccacc       60

gaggccaaga tggacttctt ccacaggttc atcgagctga acggcctggc cgccgagatc      120

gagacccact tcctgaacct gaagaacgac aagaacggcg agtccgtgta caacaaggtg      180

ctgtccaact ccaaccactc caggccgttc tccaccccgc tgctgggcac catgaccggc      240

tccaccaagg tgaccgacaa gaacgccctg tacggcaacg acctggacca ctgcaggaag      300

aagaagatcg tgccgttctc ctcctcctcc ccgctgtcct cccaggagaa gttcttctgc      360

atcgaggccg tgttcaggag ggccaagtcc cacatggagt gcaagaagct gttccaggac      420

gagaccaaca ggatggactc ccagatcaac ggcatcctga acgagctgcc gtacggcgtg      480

gagctgtcca acatgctgtc cgagctgatc gccatcccgt tcgccatcgg ctggaagctg      540

gagggctacc tgggccaggt gttcttcccg tccatcgccg agggcctgac cccgccgaag      600

tccgccaaga tcaagggcag gaggaggtcc atcgactact ccgtgaccga cgaggcctac      660

gacatcctga tgaagtactc caacctgcac tcctccttcg agaccggcct gaagatgtcc      720

aacctgttct ccgccttcta caagaagtcc aacaggaagg acgagatcca gttcaccccg      780

atctccatgg agtccaggtg cgacctgctg ctgggcaaga acttcctgaa gttcgacctg      840

aagaactgcg accacaggtc cggctccctg atgctgacca tcaacgacaa gaacaggctg      900

aacggcgact acgagatcag ggtgggctcc gacaagaagg actcctacct gaccggcgtg      960

aacgtgacca acctgggcga caacgtgttc aacctgaact acaaggtgaa cggcaagagg     1020

gagtacaaca tgctgctgaa ggagccgtcc atccacatca agatgcacag gatgagggac     1080

gacggcaact acctgtcctc cgacttcgac ttctacatga tcttctccat gtcctccgag     1140

aaggacgagg agaagctggc caggtcctgg gacatgaggg ccgccatgtc caccgcctac     1200

ggcaccgaca tcaagaagta ccactcctcc ttcccgtgca ggatcctggc ctgcgacctg     1260

ggcgtgaagc acccgtactc cgccgccgtg atggacatcg gccagctgaa cgagaacggc     1320

atgccggtgt ccgtggacaa ggtgcactgc atgcactccg agggcgtgtc cgagatcggc     1380

cagggctaca accacctgat ccagaagatc ctggccctga actacatcct ggcctactgc     1440

agggagttcg tgtccggcac cgtggacgac ttcgacaaga tcgactacaa gctgtcccag     1500

ctgtcctaca agcaggagga cctgctgatc aacctgcagg agatgaagga ccacttcggc     1560

aacgacatgc aggcctggaa gaagtccagg acctgggtgg tgtccaccct gttcttcgag     1620

ctgaggcagg agttcaacca gctgaggaac cagaggccgg gcaagaagac cgtgtccctg     1680

gccgacgagt tccagtacat cgacatgagg aggaagttca tctccctgtc caggtcctac     1740

accaacgtgg gcaggcagtc ctccaagcac aggcacgact cctaccagac ccactacgac     1800

gtgatcaaca ggtgcaagaa gaacctgctg aggaacatct gcaggaggat gatcgacatg     1860

gccgtgcaga acaagtgcga catcatcgtg gtggaggacc tgtccttcca gctgtcctcc     1920

cacaactcca ggagggacaa cgtgttcaac gccctgtggt cctgcaagtc catcaagaac     1980

atgctgggca tcatggccga gcagcacaac atcatcatct ccgaggtgga cccgaaccac     2040

acctccaaga tcgactgcga gaccggcaac ttcggctaca ggtactcctc cgacttctac     2100

tccgtgatcg acggccagct ggtgaggagg cacgccgacg agaacgccgc catcaacatc     2160

ggcaacaggt gggcctccag gcacaccgac ctgaagtcct tcaactgcag gcagatctcc     2220

atcgacggca ggaaggtggc cttcccgtac gccaagggca agaggaagtc cgccctgttc     2280

ggctacctgt tcggcaactg caagaccgtg ttcgtgtccg acgacggcga ctcctacacc     2340

ccgatcccgt actccaagtt caggaagtcc atctccaagg acgaccacga cgtggtgaac     2400

tacctgcacg acctgaccat gaacaagaac gtgatcaggg tggagtacaa caagtccatc     2460

aagtccgcct ccgtggagct gtacctgaac gacgacaggg tgatctccag gtccctgagg     2520

gacaaggagg tggacgccat cgagaagctg gtgtccaggg gctccctgat caacgagtcc     2580

ggcccgtccc tggagcacga cgaggtgaag tccgtgaccc actga                     2625


<210>  23
<211>  2613
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.5

<400>  23
atgaaggtgc acgagatccc gaggtcccag ctgctgaaga tcaagcagta cgagggctcc       60

ttcgtggagt ggtacaggga cctgcaggag gacaggaaga agttcgcctc cctgctgttc      120

aggtgggccg ccttcggcta cgccgccagg gaggacgacg gcgccaccta catctccccg      180

tcccaggccc tgctggagag gaggctgctg ctgggcgacg ccgaggacgt ggccatcaag      240

ttcctggacg tgctgttcaa gggcggcgcc ccgtcctcct cctgctactc cctgttctac      300

gaggacttcg ccctgaggga caaggccaag tactccggcg ccaagaggga gttcatcgag      360

ggcctggcca ccatgccgct ggacaagatc atcgagagga tcaggcagga cgagcagctg      420

tccaagatcc cggccgagga gtggctgatc ctgggcgccg agtactcccc ggaggagatc      480

tgggagcagg tggccccgag gatcgtgaac gtggacaggt ccctgggcaa gcagctgagg      540

gagaggctgg gcatcaagtg caggaggccg cacgacgccg gctactgcaa gatcctgatg      600

gaggtggtgg ccaggcagct gaggtcccac aacgagacct accacgagta cctgaaccag      660

acccacgaga tgaagaccaa ggtggccaac aacctgacca acgagttcga cctggtgtgc      720

gagttcgccg aggtgctgga ggagaagaac tacggcctgg gctggtacgt gctgtggcag      780

ggcgtgaagc aggccctgaa ggagcagaag aagccgacca agatccagat cgccgtggac      840

cagctgaggc agccgaagtt cgccggcctg ctgaccgcca agtggagggc cctgaagggc      900

gcctacgaca cctggaagct gaagaagagg ctggagaaga ggaaggcctt cccgtacatg      960

ccgaactggg acaacgacta ccagatcccg gtgggcctga ccggcctggg cgtgttcacc     1020

ctggaggtga agaggaccga ggtggtggtg gacctgaagg agcacggcaa gctgttctgc     1080

tcccactccc actacttcgg cgacctgacc gccgagaagc acccgtccag gtaccacctg     1140

aagttcaggc acaagctgaa gctgaggaag agggactcca gggtggagcc gaccatcggc     1200

ccgtggatcg aggccgccct gagggagatc accatccaga agaagccgaa cggcgtgttc     1260

tacctgggcc tgccgtacgc cctgtcccac ggcatcgaca acttccagat cgccaagagg     1320

ttcttctccg ccgccaagcc ggacaaggag gtgatcaacg gcctgccgtc cgagatggtg     1380

gtgggcgccg ccgacctgaa cctgtccaac atcgtggccc cggtgaaggc caggatcggc     1440

aagggcctgg agggcccgct gcacgccctg gactacggct acggcgagct gatcgacggc     1500

ccgaagatcc tgaccccgga cggcccgagg tgcggcgagc tgatctccct gaagagggac     1560

atcgtggaga tcaagtccgc catcaaggag ttcaaggcct gccagaggga gggcctgacc     1620

atgtccgagg agaccaccac ctggctgtcc gaggtggagt ccccgtccga ctccccgagg     1680

tgcatgatcc agtccaggat cgccgacacc tccaggaggc tgaactcctt caagtaccag     1740

atgaacaagg agggctacca ggacctggcc gaggccctga ggctgctgga cgccatggac     1800

tcctacaact ccctgctgga gtcctaccag aggatgcacc tgtccccggg cgagcagtcc     1860

ccgaaggagg ccaagttcga caccaagagg gcctccttca gggacctgct gaggaggagg     1920

gtggcccaca ccatcgtgga gtacttcgac gactgcgaca tcgtgttctt cgaggacctg     1980

gacggcccgt ccgactccga ctccaggaac aacgccctgg tgaagctgct gtccccgagg     2040

accctgctgc tgtacatcag gcaggccctg gagaagaggg gcatcggcat ggtggaggtg     2100

gccaaggacg gcacctccca gaacaacccg atctccggcc acgtgggctg gaggaacaag     2160

cagaacaagt ccgagatcta cttctacgag gacaaggagc tgctggtgat ggacgccgac     2220

gaggtgggcg ccatgaacat cctgtgcagg ggcctgaacc actccgtgtg cccgtactcc     2280

ttcgtgacca aggccccgga gaagaagaac gacgagaaga aggagggcga ctacggcaag     2340

agggtgaaga ggttcctgaa ggacaggtac ggctcctcca acgtgaggtt cctggtggcc     2400

tccatgggct tcgtgaccgt gaccaccaag aggccgaagg acgccctggt gggcaagagg     2460

ctgtactacc acggcggcga gctggtgacc cacgacctgc acaacaggat gaaggacgag     2520

atcaagtacc tggtggagaa ggaggtgctg gccaggaggg tgtccctgtc cgactccacc     2580

atcaagtcct acaagtcctt cgcccacgtg tga                                  2613


<210>  24
<211>  2895
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.6

<400>  24
atgtccgcca acagggtgtc cgccaactcc cagttcgagc tgggctaccc gatgtccctg       60

tccctgaggg gcaaggtgtt caactccagg gagatgatga aggagatcct gccggtgatg      120

aacaacatcg tgcactacca gaacaacctg ctgaagctga tgctgatcct gaggggcgag      180

aagtacaccc tggacggcca gttcttctcc cagaaggacg tggacaggca gttcggcgac      240

ctgtgcaagg agcacaacat caagggctcc atctgctccc tgaaggagaa gtccaggaag      300

ctgtacgagg tgttctcctg ctacatcgac aagaagggca acctgaagac caactccaag      360

gccaggtcct tcgccggcgt gctgctgaac ccgaaggacg tgaagctgcc gccgcagatc      420

gactccatct cctccttcgt ggtggagctg agggccaagg gcgtgctgcc gatcaagcac      480

gagggcaact acctgtccgg ccacccgtcc ctgaagtact ccgtggccca gaacgtgctg      540

gtgaagctga cctccatgga gaagctgcag aagatctact ccgacgagaa ggccggctgg      600

gagaacatcg tgtccgaggt gaggtccgac ctgccgaaga tcgagaggta cgagaggatg      660

ctgctgtcca tcaaggccgt gaaggagatg gagaagttcg gcatcaacaa ctacaggcac      720

ctgctgaaca actggaggga cgaggtggac aaggactccg gcaaggtgct gaagcagggc      780

atgaggacct acttcgtgaa catgctggag tccaagaagg actacaggtt cgaggagtcc      840

gacaggtacc tgttcggcta cgccccggag gtgatgaacc tggtgtacca cgacttcagg      900

gacctgtggc agggcgagga catcatcggc tcccagtccc cggagaagaa ggacagggac      960

tacgtggacg tgatcttcaa ctacttcaac tggaggaagg agtccatcaa catctcctcc     1020

ttcgactcct acggcaagac cgcccagatc aagctgggcg acaactacgt gccgttctcc     1080

aacttccagt acgacaagat cctggacgcc tggaccctgg agatcgccaa cgtgtccggc     1140

gagggcgaca accacaagct ggtgatcgcc aggtccccgc agttcgactc ccactcctcc     1200

gtgaaggaca tcgtgatgaa gaacctgaag ggcaaggagg cctccaagac caccctggag     1260

ttcaggtact ccggcgactc caagaagtcc acctggtaca ggggcaccct gaaggagccg     1320

accctgaggt actcctcctc caagaactgc ctgtacgtgg acttcgccct gtccaaccac     1380

atcgtggagg gcctgatctc cgacaacctg ggcatctccg acaagatgta caagttcagg     1440

ggcgagttca tgaaggcctc cccgtcctcc ggcaagcagt ccaactccat caacctgccg     1500

atcaagaagc tgagggccat gggcgtggac ttcaacctga ggaggccgtt ccaggcctcc     1560

atctacgacg tggagaacaa gaacggcaac ctggagttct ccttcgtgaa gcacgtgcag     1620

tccttctcca acgagaacga cgaggagagg gccaaggagc tgctgaacat cgagaggaac     1680

atcctggccc tgaagatcct gatctggcag accgtgggct acgtgaccgg caagaacgac     1740

accatcgacg gcgtggtgac caggaagaac aacgccgtgg acatcgagaa gaccctgggc     1800

atcaacatga aggagtacat ggcctacctg aaccagttca ggtcctacga ggacaagaac     1860

aaggccttca tggacctgag gaagagggag tacgcctgga tcgtgccgcc gctgatcttc     1920

cagtgcaggt ccaggctgat ctccttcagg tccgagtact tcaacacccc gaaggacgag     1980

aagtcccact actgccagca caggaacttc gtggactact ccaccttcct gaagaagaac     2040

gtggtgaaga agatgatgga gctgaggagg tcctactcca ccttcggcat gtcctccgag     2100

cagtccatct gggtgaccaa caacgaccac gccaaggacg gctccaagaa gaacggcaac     2160

atgttcgacg acgacctgca ccagtggtac aacggcctgg tgaggaagtg ctcctccctg     2220

gcctcctcca tcatcaacgt ggccagggac aacggcgcca tcctggtgtt catcgaggac     2280

ctggactgcc acccgtccgc cttcgactcc gaggaggaca actccctgaa gtccatctgg     2340

ggctggggct ccatcaaggc ctccctggcc caccaggcca ggaagcacaa catcgccgtg     2400

gtggccaacg acccgcacct gacctccctg gtgtcctcca ccaccggcga gctgggcatc     2460

gccaagggca gggacgtgct gttcttcgac tccaagggca agctgacctc caaggtgaac     2520

agggacgaga acgccgccca gaacatcgcc atcaggggct tcgtgaggca ctccgacctg     2580

agggagttcg tggccgagaa gatcgaggag aacaggtaca gggtggtggt gaacaagacc     2640

cacaagagga aggccggcgc catctacagg cacatcggct ccaccgagtg catcatgtcc     2700

aagcaggccg acggctccct gaagatcgac aagaccgagc tgaccccgct ggagatcaag     2760

atggagaaga agaacgacaa gaagatgtac gtgatcctgc acggcaagac ctggaggctg     2820

aggcacgagc tgaacgagaa gctggagaag gacctggaca accacctgaa gtccaagtcc     2880

tccgtgatct cctga                                                      2895


<210>  25
<211>  2889
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.7

<400>  25
atgtcctccg ccaacgacca gctgggcctg ggctacccgc tgaccctgac cctgaggggc       60

aaggtgtaca accacgacac cgccatggag gccttcgccc cggtgatgaa gggcatggtg      120

ccgtacgcca acaacctgat gaggatcctg ctgaccctga ggctggagaa gtacaccctg      180

gacggcatcc accacaccaa ggaggaggtg gagaaggacc tgaggggcct gatgaaggag      240

tacggcatca acctgtcctt cgccaagttc tccgagatgg ccggcgaggt gtacagggtg      300

ttcgtgtgct acgtggacgc caagggcaag ctgaaggtga acggcaaggc caggggcttc      360

gccaacgtgt tcttctccga ggacgacgcc accatcccgg agaactgccc gtccatggag      420

ctgctgagga agaagggcat gttcccgatc ctggtggacg gcaagccgat ctcctccatc      480

tccagggaga agaccccgct gaagtactcc gtggcccagg acgtgctgac caagctgacc      540

tccatggagg agatctccaa ggagtacgag aaggccaaga ccgactggga gaacgagtgc      600

cagaaggtga tctcccagct gccgctgatc ggcaggtacg aggccctgct gaccaccatc      660

ccgctgatcc cggagatgag gggcttcgac ggcgacaact acaggaagat gctgaacagg      720

tggagggact acgtgaacga ggacggcgag ctggtgaggg gcggcatgaa gacctacttc      780

ctggacctgc tgtccaagga cacctcccac aagttcaacg aggaggagag gtacctgttc      840

ggctactgcc cggagttcat gaacctgatc taccacgact tcagggacct gtggtccaag      900

gaggacatca tcggctccca gaggaagggc aagggcctga agggcaagga ctacgtggac      960

gtgatcttca actgcttcca ctggaggagg gagtccatca acatctcctc cttcggcaac     1020

aacgacaagg tgatgaacat ccacctgggc gacaacttcg tgccgttcga gctgaagtcc     1080

cagaacggca tctgggaggt gcacgtgcag aacctgcacg gccagaacga cccgcacagg     1140

gtgatcgtgt gcaggtgccc gcagttcaac gaggactcct ccatgaagat ggtgcacccg     1200

ctggccaaga acggcgagga gtccgacaag gagaacatcg agttcaggta ctccggcgac     1260

tccaagaggg agacctggta caccggcctg ctgaaggagc cgaccctgag gtacgacgtg     1320

gagaggaagt ccctgtacgt ggacttcatc ctgtccaacc acagggtgga gggcgtggtg     1380

accaacgagt acctgaagga cccgagggac ctgttcggcg tgaggggcta cttcctgtcc     1440

tcctccgtgt ccaacccgag gcagaaggac aagacctccc tgccggacgg caagttcaac     1500

gtgatgggcg tggacctggg cctgaagtgc ccgtacgagt gcgccatcta cggcatcacc     1560

gtgaagaacg gcaagatgca gcacaagtgg tcccacaacg tgtccgccga ggacaacaac     1620

aacgtgtccg agaggctggc caacctgaag aagatcgacg agaagatcct ggccacccag     1680

gtgctgatct ccctgaccaa gatgtgcgtg gtgaaggacg aggagatccc ggactcctac     1740

accctgaggg agcacagggt ggacatcgcc aagtccctgg acctggacat ggacaagtac     1800

aggaggtacg tggagaagtg caagaagaac ccggacaaga tccaggccct gaaggacatc     1860

aggaagtccg agaacaactg gatcgtggcc gagaagatca acgagatcag gtccctgatc     1920

tccgagatca ggtccgagta ctacgcctcc aaggacaaga ggaactactg caggaacctg     1980

aacggcgtgg acctgtccgt gttcctgaag aagaaggtgg tgaagaactg gatctccctg     2040

ctgaggtcct tctccacctt cggcatgacc ccgcaggagt ccgcctacat caggaaggac     2100

ttcgccaaga acctgtccaa gtggtacaag ggcctggtga ggaagtgcgg ctccatcgcc     2160

gcccacatcg tgaacatcgc cagggacaac aaggtgatgg tgatcttcat cgaggacctg     2220

gacgccagga cctccgcctt cgactccaag gaggacaacg agctgaagat cctgtggggc     2280

tggggcgaga tcaagaagtg gatcggccac caggccagga agcacaacat cgccgtggtg     2340

gccgtggacc cgcacctgac ctccctggtg aaccacgagt ccggcctgct gggcatcgcc     2400

ggctccggca acgacaggaa catctacacc ttccagaaga acaagaagta cgtggtgatc     2460

aacagggaca acaacgccgc ccacaacatc gccctgaggg gcctgtccaa gcacaccgac     2520

atcagggagt tctacgtgga gcagatcgac gtggaccact acaggctgat gtacggcccg     2580

gaggccgaga acggcaagag gaggtccggc gccatctaca agcacatcgg ctccaccgag     2640

tgcgtgttct ccaagcagaa gaacggcacc ctgaaggtgg agaagacctc cctgaccaag     2700

gacgagaagg agatgccgaa gatcaacggc aagggcgtgt acgccatcct gcacggcaac     2760

gagtggaggc tgaggcacga gctgaacgag gagctgggcg ccaagctgga cggcatctcc     2820

gtgaagaggg tggtgtccga gccgaacaag gtgaagacct ccctggtgaa gggctccgtg     2880

agggcctga                                                             2889


<210>  26
<211>  2724
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.8

<400>  26
atgaagaagc agaccatcgt gaagaaggac tccaaggccg agaccaagga gaacaagatg       60

tacccggaca aggacaccga cttcccggtg aactcccagt tctccaggtc catctccatc      120

agggccaacg tggacccgaa ggacctgctg gtgctgaaga ggaccttcga ggagaccacc      180

aagatctccg acgagctgct gtccaccctg ctgatgctga ggggcaagga ctactgcctg      240

gacaacgtgg tgtgcaaggg cgaggaggtg ctggagaacc tgtacaagaa gctgtccaag      300

aacgccaccg tgaacaggga caagttcatc tccaccgcca aggccttcta cgagtacttc      360

cacggctgct cctaccacaa gggcttcaag tccttcttct tctcctccaa ggagatcgac      420

tccatccagt ccgagaagtt cggctacctg agggagatcg gcctgttccc gatcaagatc      480

gacgcccaga tctccaacga cctgcagtac tccatcgtgg cctccaacca cgccaagatc      540

aagggcttcg agaagatcga caaggagtac caggccaaca aggagaagtg gaacaagacc      600

atcggcgagt ccaccctgaa gcacctgaac aggtacggcg agatgctgaa gggcctgtcc      660

gacctgggca ccatgggcaa cttcaacggc aagaagtacg acaggttcat gggccactgg      720

aggaacgagc agaagatccc ggaccacatc tccatgctgg acttcttcag gaagatctac      780

caggagaagg gcaagtccca caggttcacc gccatcgaca acttcaccta cggctacgag      840

tccgagttca tgaaccacat ctacctgaac ttctccgacc tgtggctgaa ggaggacgtg      900

atcggcgacg aggagtacgt gtccctgatc aggggcgcct accactggca gaaggacgtg      960

gtgggcatcg cctccttctc cggctacaac aagtacgaga agctgttcat gggcgacaac     1020

aagatcaact acgccctgga cttctccaac aaggaccagt ggctgatgaa gttcaacaac     1080

gtgatctcca aggagccgga gaccatcacc ctgaggctgt gcaagaacgg ctacttcaac     1140

aacctgtccg tgctggagaa gaacgacgag aacggcaggt acaagatcag gttctccacc     1200

gagaagcagg gcaagtactt ctacgaggcc ttcatcaggg agccgttcct gaggtacaac     1260

aaggacaacg acaagatcta cgtgcacttc tgcctgtccg aggagatcaa ggagaactgc     1320

ccgaaccacc tggacaccag gtccgacaag tacctgttca agtccgccct gctgaccaac     1380

tccaggcaga agctgggcaa gctgcactac agggacttcc acatcgtggg cgtggacctg     1440

ggcatcaacc cggtggccaa gatcaccgtg tgcaaggtgc acgtggacaa gaacgagaac     1500

ctgaagatca ccaagatcat caccgaggag accaggaaga acatcgacac caactacctg     1560

gaccagctga acctgctgta caagaagatc gtgtccctga agaggctgat cagggccacc     1620

gtggccttca agaaggacgg cgaggagatc ccgaagatgt tcaagatggg caagaagtcc     1680

ccgtacttcc tgaactggac cgaggtgctg aacgtgaact acgacgacta catcaaggag     1740

atctccacct tctccgtgga caggctgtcc ggcctgaccc tgccgatgca gtgggccagg     1800

tcccagaaca agtgggtggt gaaggacctg accaagatgg tgaggaaggg catctccgac     1860

ctgatctacg ccaggtactt caactgctcc gacaagaccc agtacgtgac cgagaacaac     1920

gccgtggaca tcaccacctt caagaagcac gacatcatct ccgagatcat cggcctgcag     1980

aagatgttct ccggcggcgg caaggacgtg gccaagaagg actacctgta cctgaggggc     2040

ctgaggaagc acatcggcaa ctacaccgcc tccgccatcg tgtccatcgc ccagaagtac     2100

aacgccgtgt tcatcttcat cgaggacctg gacctgaaga tctccggcat gaacggcaag     2160

aaggagaaca aggtgaagat cctgtggggc gtgggccagc tgaagaagag gctgtccgag     2220

aaggccgaga agttcggcat cggcatcgtg ccggtgaacc cggagctgac ctcccagatg     2280

gacagggaga ccttcctgct gggctacagg aacccgacca acaagaagga gctgtacgtg     2340

aagagggacg acaagatcga gatcctggac gccgacgaga ccgcctccta caacgtggcc     2400

ctgaggggcc tgggccacca cgccaacctg atccagttca gggccgacaa gatgccgaac     2460

ggctgcttca gggtgatgcc ggacaggaag tacaagcagg gcgccctgta cggctacctg     2520

aactccaccg ccgtgctgtt caaggacaag ggcgacggcg tgctgaccat ccacaagtcc     2580

aagctgacca agaaggagag ggactccagg ccgatcaagg gcaagaagac cttcgtggtg     2640

aagaacggca agaggtggat cctgaggcac gtgctggacg aggaggtgaa gaagtacccg     2700

gagatgtaca actcccagaa ctga                                            2724


<210>  27
<211>  2739
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.9

<400>  27
atgtccgact acaagttctc caacaacggc gtgaccaaca ccggctccgc ccacatcggc       60

ctgtccccgg agaactcctc caccgtgatg gacatgttca aggtgatcac caaggacgcc      120

gacttcctgc tgaagaacct gctgatcatg gagggcggcg agtacatgct gaacagggag      180

atccacaacg gcgacaagga gttcgacaag atcatctcca agctgggcct gtccaagaag      240

gagaaggaga acctgaagat gaagtgcaag gacttcttct tcgacttcgt gaagctgcag      300

aacggcaggt ccctggccaa catcctgttc gagaccaagg gcaccaccct gatcggctgc      360

ggcaaggaca agaagggcga gaaggtggac ggcgagtacc cgaccatcta ccacgaccac      420

gagaccctga ggtccaccgg cctgctgccg ctgaagttct ccaagaacat cgacgacgtg      480

gactacaagt acctgatctg ctacctggtg cacaacgtgc tgtcctcctt catcgagaag      540

agggacgcct acaacgacaa caagaaggag tgggagtcca agctgtccaa ctccaacctg      600

ccgcagctgg agaggatgtc cgagttcctg aacggcatca accacctggg caacatcatc      660

ggctggaacg gcaagaagta catcggcttc atcaagaagt ggaccgacga ggagtcctcc      720

atgtacgact tcttcgtgca gaagctgcag gacaacccga agtacaagtt cggcaagaag      780

gaccagttcc tgtacggcta cgagccggag ttcctgaact acctgttcca cgacttcagg      840

gacctgtggc acccggacaa cctgatcggc aaggacgagt acgtggacct gatctccggc      900

aagaacaaca ccgacgccga gaccgccaac aagggcgcct accactggct gaaggacttc      960

atcaacatct cctccttcga cgcctacggc aagatggcca ccatcggcat gggcaacaac     1020

ctgatcaact actccatgaa catcgacaag gacggcaaga tcatcgtgaa catggacaac     1080

atcttcgaca ggtccaagcc gatcgtgttc aacgtgtaca ggaactccta cttcaggaac     1140

ttcaagatca tcgagtccga cgacaagaag ggcatctaca aggtggagtt ctccacctcc     1200

aacaacggcg tgatctacga gggctacatc aagtccccgt ccctgaggtt cgccaccaag     1260

ggcggcacca tcaagatcga cttcccgatc tccgacaaga ggatcaaggg cggcagggag     1320

atgaacaccg acctgatgtg gttcctgaac agggcctccc cgtgctccac caagaacaag     1380

gaggtgaact ccttcatcgg caagaacttc gtgggcctgg ccatcgacag gggcatcaac     1440

ccgctgatgg cctggtacgt ggccgagtgg acctacgaca aggacggcaa ggccaagatc     1500

gtgaggtcca tcgccaacgg cagggtggac tccggccaca acgagtccga ggtgaagttc     1560

gtgagggaga ccaccaacag gatcgtgggc atcaagtccc tggtgtggaa caccgtgaag     1620

tacaggaccg gcggctccga gggcatcgac aggtgcagga agtcccagaa cggccaggtg     1680

gacctgttcg agatgttcga catcgactac aacaactacc tgaaggaggt gaacaacctg     1740

ccgtacgacc cgaactccga gaggtccatc atccagacct gggtgtcctc cccgtggaag     1800

gtgaaggacc tggtgaagga cgccaagaac aggatggtgc agatcaagac ccagtaccac     1860

aacgccaagg acaaggagaa gtacatcacc acccagaaca gggccggctt ctacgacttc     1920

ctgaagatcg agatggagaa gcagttcacc tccctgcaga ggatgttctc cggcggccag     1980

aaggacatct gcaagaacaa cgaggagtac aggaggggcc tgaggaggag gatcaacctg     2040

tacacctcct ccgtgatcat gtccctggcc aggaagttca acgtggactg catcttcctg     2100

gaggacctgg actcctccaa gtcctcctgg gacgacgcca agaagaactc cctgaaggac     2160

ctgtggtcca ccggcggcgc cgacgacatc ctgggcaaga tggccaacaa gtacaagtac     2220

ccgatcgtga aggtgaactc ccacctgacc tccctggtgg acaacaagac cggcaagatc     2280

ggctacaggg acccgaagaa gaagtccaac ctgtacgtgg agaggggcaa gaagatcgag     2340

atcatcgact ccgacgagaa cgccgccatc aacatcctga agaggggcat ctccaagcac     2400

atcgacatca gggagttctt cgccgagaag atcgaggtgt ccggcaagac cctgtacagg     2460

atctccaaca agctgggcaa gcagaggatg ggctccctgt actacctgga gggcaacaag     2520

gagatcctgt tcggcctggg caagaacggc gagccgatcg tgtgcaagag gggcctgtgc     2580

aagaaggaga ggctggcccc gaggatcgcc gagaagaagt ccacctacct gatcatgaac     2640

ggctccaagt ggatgttcag gcacgaggcc aagaagatcg tggagaccta caaggacagg     2700

tactgcgcca accacaaggt ggcctccaag gacggctga                            2739


<210>  28
<211>  3360
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.10

<400>  28
atgatgaaca tcaacgagat ggtgaagctg atgaagtccg agtacctgtt cgaggacgac       60

ggcatcgtga ccaagaacaa gatccaggag aggctgagga acggcttctc cgacatcggc      120

gtggacccgt ccctggtgtc ctacgcctcc aagttcctgg actccatgtt catctgcttc      180

tccagggtga agggcgagaa gaacttcaag gccaagaacg tgaggaagaa catgtcctcc      240

gccgagaaga aggcccagaa gaagaaggag taccaggagt actaccaggg cgtgatggcc      300

cagcaggacg cctacgccca gctgctgtcc gacccgaccc aggagaacct ggacaagctg      360

aacgagctga tctccatgtc cgtgaacggc tccctggtgg aggacttctt cccggccctg      420

aagaacatga tccagaaggc cgactactcc atcgacaaga agggcctgct ggacttctcc      480

tgctgcatga tggacaggta cgaggacagg tccctgacca gggccatctc catctccgcc      540

ttcaacatcc actccggcgg cctgaggaag gccctgtccg acatctccga gaaggtgcag      600

gacctgtcca acaccctgct gatcaggatc ctgtacatga agggcgagga gctgtccatc      660

gacggcgaga agatctccaa ggaggaggtg cagaggcagc tgaaggccga ctacgaggag      720

cacaaggagt acttcgagga cttcgaggac ttcgccaaga agtgcaggtt cttctacaac      780

aagttctcca agaagaagaa gaccaggggc ttcggcacct acttcttcgg cgacaagaag      840

aaggagatct cctccgccga gtacaaggcc cacaaggagc tgagggactc cggctacctg      900

tggttcgaca tcggctggtc cgagtcctcc gacttcaagt acgtgatcgt gggcaacgtg      960

tccggcaagc tgaagtcctt cgaggagacc tccgaggagt accagaagtc caagaactgc     1020

tgggaggccg agagggtgaa gctgtacgag caggactccg acttcgtgct gttcgtggag     1080

gacatgatcg agtccaagta cggcccgatc gagaagatga agctgaggac cttcaagacc     1140

atcgtgaaga agctggacaa ggagttcggc aagaggggcg acaagacccc gtccatccac     1200

gactacttcg agtccctgga cccgaaccac accttctccc agtccgagca gttcatgtac     1260

ggcctggacg tgaccctgat gcagttcctg ttcaacaaca agaagcagtt ctacaagctg     1320

tgcaaggacc acgacggcaa gaggaccttc gccaaggtgg tggaggagtc ctaccactgg     1380

ggcaagaact ccatcaacgt gtccaccttc cagaactcca cctccatcct gctgggcggc     1440

aactacctga actactccat gtccatcgag ggcgagggcc tggtgatcaa gttcgacaac     1500

ccgctgtccg gcaaggaggt gcacttcgtg gtgtgcaaca acaagtacct gtccgacctg     1560

gagatcctgt ccggcaaccc gaacaggaag gacaacaact acaccatctc ctactccacc     1620

ggcggcaagg ccaggttcat cgccaagtcc aaggagccga ggatcttctt caacaggaag     1680

accaagaagt gggagatcgc cttccagctg tccgacgtgt ccccgctgaa cggcaagttc     1740

ggcaagcagg gcgagttcct gtccaacctg aggaagttcg tgtacaacca cgtggccaag     1800

tccccgtcca agctgaacat ctccgacaac aactgcaggg ccgtggccta cgacctgggc     1860

atcaggaacg tgggcgcctg gtcctccttc gacttctcct acaaggacgg cgtgctgggc     1920

ggctacaagt acctgacctc cggctccctg aggtccaagt ccgagtcctc cgagatggac     1980

cagggctact acttcgtgct gaacctgaag aagatcgtga agctgatccc ggtggtgaag     2040

aagtccatca tcgacgaccc ggagctgaag aggcagttca tcggcgtgct gaacgagaac     2100

ggcaacaccg tgggcctggg caacatcggc aagctggaca tcgcctccag gaaggccgtg     2160

cagtccttcc acaactgcat ccagcagatc aactactacg tggacaccta cgccgaccac     2220

atcgacaaga tctccgccaa ggacttcgtg gacgacatcg acggcatcaa ggtgctggac     2280

gaggacgacc cgtacgtggt gaagatcctg tcccacctgc cggaggacgt ggagggcaac     2340

caggacgaca tcctgaacat ctccctgctg aagtggaaga cctccaacgc ccagttcgtg     2400

ccgccgctga tccaggaggc caaggccatc atgtccagga tcaagaggga gaacctggac     2460

aacatcaggg gcaagaagac ccaggtggtg acccagaaga ccttccacaa gatcaagttc     2520

gccaaggccc tgctgtccct gatgaagtcc tggtcctcca tcggcaccgt gagggtggtg     2580

aagaccgacc agatctacgg caagaagatc tgggactaca tcaacggcct gaggaggaac     2640

gtgctgacct acctgtcctc cgccatcgtg aacaacgccc tggacctggg cgcccacatg     2700

atcatcctgg aggacctgga ctcctccgtg tccaagtaca gggagaagga caagaacgcc     2760

atccagtccc tgtggggctc cggcgagctg aagaagagga tcgaggagaa ggccgagaag     2820

cacagggtgg tggtgcagta cgtgtccccg tacctgacct cccagctgga caacgagacc     2880

aaggacatcg gctacaggaa gggcggcagg ctgtacgtgg tgaggaacgg caagatcaag     2940

tccatcgacg ccgacatcaa cgcctccaag aacatcggcg agaggttctt cgacagggac     3000

ctgatccaga ccctgtccgg cgtggtggtg gaggaccagt ccaccgtgta catcctgcag     3060

aagaggaacg tgtcctccga caacaggaag aggttctaca agaagttcct ggaggacgtg     3120

ggcggcaagt ccaagaagga cgccgtgctg aagatgggcg accacggcga gctggaggtg     3180

gagaggctga tcgacggcaa gaagctggac atcgacggca agaagatcct ggtggacggc     3240

gagaaggtgc cgttcaggaa cacctccgtg tactactccc cgaagaagaa gaagtgggtg     3300

tccaaggagc tgaggtgcaa ccacatcaag ctgaccgtgg aggagcagga catcaagtga     3360


<210>  29
<211>  3408
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.11

<400>  29
atgaacaact acgacaacta cctgtccgac tacctggcca tgctgccgca caccaagagg       60

accgagatca agaagaccgc ctccaagatc tccaggaagc tgaaccagaa ggaggtgaag      120

aagcagatcg agaggtccga gtacatcagg tccaactgcg gctacatcaa catcgagagg      180

ccgcagaagt ccctgtcctt cctgtcctac tccaccatca agtccgcctg catgtccgtg      240

aacttcaggg ccttccagaa cccgatcaac gactacgaga ccgccatctg caacggcatc      300

aacgagtgcg agaggttctt ctaccagcag atcgactcca tctacatgtc ccagatcatc      360

gagcagctgt tcgacttcta catcgcctcc aggcagcacg acatgttcat caacaacacc      420

gtggtgccgt acgacgtgaa caagctgaag tcctactaca ccgccaacga gaagtactcc      480

ttcgagcagt tctgcgacga catcaaggag ttcaccaaca agggcttcac ctccggcggc      540

gtgtcctgca tcctgaacct gttctacaag ggctccgtga aggactccaa gaacaagaag      600

gactacatca agtccgtgaa gaggctggag accaacggcc tgttcaagaa gctgaacatc      660

ttcgagaaga acggcatctc caagtacttc gccgcctcca ccctgtccac cttcttcgcc      720

accatctcct cctggaagaa gcagaacgac gactggaccg gcgtggccaa ggacggcacc      780

tccctgctgt ccaagctgga gaacaagacc atcaccctgc agtccatcat caagcaccac      840

agggtgatca acgagctggc cgtgctgatc gtgaaggcct acaaggaccc ggtgaagacc      900

ctgaacaacc tgttcgagga gaggtccgac aacaacaacg acttcaagta cacctgctcc      960

gacgacgagg acaagtaccc gatgtacatc aagagggaga tcgccgagtt cgtgaagaag     1020

cacaagaccg tgtgggagga gatcaggtac ttcgacgagt ccgacaccaa gaagaagaag     1080

agggacaaga aggagtcctc ctccgacgac aagtcctacc tgtgctgcgg cgactcctgg     1140

gactacctga agacctgggt gaggctgtac ggcgagtact acttcttcga caacgccctg     1200

aaccagttcc tgaggaagcc gtccgcctcc atgcacctgt acacctccct ggactggatc     1260

aacaagaaga ccatctgcat cgtgggcgcc aactactaca agatcggcaa ggtggaggtg     1320

gtggagagga acaaccagag gttcctgctg gtgtacgtgt ccgtgccgga gatggagaac     1380

tacatcatca tcccgctgca gctgaacaag tacttcggca acttccagtg caagatcttc     1440

gagggcaggc tgcaggccat cttcaagagg tacgccaact tcaacgccct gaagaacaac     1500

aagccgcagc cgtccccgaa catctccgtg aggatcaacg agttccactt cgccctgagg     1560

tcctacagga agcagcagat ctccgccgag gacttctcca agggcaggtt ctccctgatc     1620

tccaagatcg gcttccagat gaccaacgac gaggtgttcg gcaggacccc gagggagatc     1680

gccctggtga aggaccacct gtccaagggc tacgtgcact tcggctccca gatcatcgag     1740

gactccagga aggaggtgga gcaggtgctg aagaagccga tgatcctgat gggcgtggac     1800

ttcggctact ccccgctggc ctcctacaac atcaagccgc tgcagaccgg caagccggcc     1860

accgactggg tgaagaacct gcacggcaac ttcctgtgcc agaacgtgtc cctgggcgag     1920

accatcaccg agggcgagat cggcgacgtg ccgaccgaca cctacacctc ctccaacgag     1980

atctactcca tcgccaccct gaccttcagg aacgccgacg gcaagctgga gaacaggtcc     2040

ttctccaggt tctaccacga gctgaacaac accctgaaca tcatcgagca gatcaagggc     2100

accttcaact tcatccactc catcaacacc cagttcaagg agatcaaggc cctgaagacc     2160

accgaggagt tctcctccta cgtgtccacc ctgacctggg accagttcat cgaggactcc     2220

aggaagaccg ccaggtactc caagtactgg atccacatca tcaacgagaa cccgaagagg     2280

aggaccatcg ccaccctgaa cgagaccctg aagctggtgg acgagaagca caggttcacc     2340

gtgaccatcc aggagatctt cgacctggtg aagtactgcc agcagcacgg ctactacccg     2400

aagtccaacg tgatgtccaa gctgaggaac ctggccatca agctgatcaa cgacctgatc     2460

aggtaccaga agatcggcat ccactcctgc tacctggact tctgcgtgct gatcaagaac     2520

cacatcgccc tgctgaactc ctccaccgcc ttcatcatca acttctccag gaacaaggag     2580

aacatcatca ggaacaacac ctccaagatc cactccctgt gggtgtacag ggacaacttc     2640

aggaggcaga tgatcaagaa cctgtgctcc cagatcctga agatcgccgc caagaacaag     2700

gtgcacatcg tggtggtgga gaagctgaac aacatgagga ccaacaacag gaacaacgag     2760

gacaagaaca acatgatcga cctgctggcc accggccagt tcaggaagca gctgtccgac     2820

caggccaagt ggtacggcat cgccgtggtg gacaccgccg agtacaacac ctccaaggtg     2880

gacttcatga ccggcgagta cggctacagg gacgagaaca acaagaggca cttctactgc     2940

aggaagcagg acaagaccgt gctgctggac tgcgacaaga aggcctccga gaacatcctg     3000

ctggccttcg tgacccagtc cctgctgctg aaccacctga aggtgctgat caccgaggac     3060

ggcaagaccg ccgtgatcga cctgtccgag aggaccaccg agccgcagaa gatcaggtcc     3120

aagatctgga ccaactccga cgtgcagaag atcatcttct gcaagcagga gaacggctcc     3180

tacgtgctga agaagggctc caccgacatc aaggagaaga tgcacaaggc cgtgctgcac     3240

aggcacggct ccctgtggta cgactacctg aaccacaaga acatgatcga ggacatcaag     3300

aacctgcacc tgtccaactg ctccctgacc acctccacca actccgacgt gatcaactcc     3360

cactccggct cctccaggtc cctggacaag accaagacct acgcctga                  3408


<210>  30
<211>  3042
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.12

<400>  30
atggcctcct ccgacgccca gaagttcccg cagacccaca acaaggtgat gtccttcagg       60

ctgaccgcct ccaacatcgg ctccgtgctg tccctgcact ccaacctgca cgacgccgcc      120

gagatcggca tcaacgagtg caggtggtgg atcggcgacg gcgagatcta cgagagggac      180

ccggcctgca ggtccatcaa gaagggcaac gacatcagga ccgtgacctc cgagaagatc      240

aaggagctgt ggaccaagca caccgaccac tccgtgccgc tggtggactt catcgacatg      300

ctgaagttcg tggcccagtg cgccatctac ggcgactcca gggccctggc ctccaccctg      360

ttcggcaagt ccaaggccga gaccaggggc gtgtccaccg aggacatgac cgtgatcagg      420

gcctggatcg ccgagaccga cgccgtgctg gcctccggcc tgtccccgaa gaagaagaag      480

aagaaggaga aggaggccgg caagaaggag aggaagccgg acgtgaagat ggagatgtgc      540

aggaggatca ggtgcaccat ggtgcagtgc ggctacttca ggaggttccc gttcgaggcc      600

aagatcgaca acggcggcga gaggggcaag atggactccg agctgtccta cgtgtccgcc      660

aggaacctgc tgaggtgcct gtccacctgg agggcctcct ccgtgatgag gagggactcc      720

tacctgatcg aggaggagag gatcaaggag gccgagtcca agatgacccc ggagatcatc      780

gacggcctga ggaggctgta caggtactgc gccgtggacc acgacttcct gaagtggttc      840

ggcggcagga tcatcaggca catcgactcc tgcctggccc cggccatcgc cggcaacacc      900

ggcaggccga ccggcggcga gtccttcacc gtgatctacg acaggaggaa gaagagggac      960

gtgaagatca cctactccgt gccggaggag atctacggct acctgtcctc ccacccggag     1020

ctggtggcca tcggcaagga cggcatgacc ccgatctcca ggcacgccga ctacctggag     1080

atgatcgcct cccacgagaa gcacaggtgg tacgccacct tcccgaccgt gggcaaggag     1140

gacggctaca ggacctccgt gctgctgggc aagaactacc tgacctacga cctgtcctac     1200

gacggcgagt ccgtgccgga caagaagatc aacgtgatct ccaagggcca gccggtgtgc     1260

ctggacctgc acgacggcag gagggtgtcc tccctgtacc tgaccgtggg cgagtccgcc     1320

gcctacgaca tcgccgtgag gaagaacaag aggcaccacg gcaagccggc cgactactgc     1380

aggatgaggg tgcacctgac ccaggagagg gaggacaaga cctacaacga cccgtacttc     1440

tccaacatgg agatctggag ggccggcgac caggtgtacg ccatcgagtt cgacaggcac     1500

ggcgccaggt acaccgccat cgtgaaggag ccgtccgtgg agtacaggaa caagaagctg     1560

tacctgaggg tgaacatggt gctggactcc ccgtccaggc aggacgacaa ggacatgtac     1620

tacgcctaca tgaccgccta cccgtcctcc aacccgccgg tggagacctc cgacaacaag     1680

aagaggttcg agaggctggg cccgggcagg agggccatcg gcggcatcga catcggcatc     1740

ggcaggccgt acgtggccgt ggtggcctcc tacgaggtgg gcccggccgg caccgagcag     1800

aagttccaga tcgaggacag gctgatcgag gacgacggct cctccccgta cgactccctg     1860

tacaacgact tcctgaccga catcaggacc gtgtccagga tcatcgaggc cgccaagaag     1920

atctccgagg gcgacctgga ggacatcccg tccgacatgt ccgtggacga ggacggctcc     1980

atcgccgcca ccatgaagag gatgtccgcc aggatcgccg agaggcacca cctgtacggc     2040

gagaggaagt ccgaggccta cgccaccttc ctgaagatga accacaagca gaggctggac     2100

atcctgctga cccagaaggc ctccaacgcc accctgaagc agctggtgga ggaggacccg     2160

tccttcctgc cgaggatctg cgtgtactac gtgatctccg tggagaggga gctgaagaac     2220

aagcacagga acgcctacct ggacggcctg accgtggacg agaagtactc cggcgagacc     2280

aagaggggct acgcccagaa gaggctgaac tccatgctga gggcctactc cgccctgggc     2340

gaggaggaga ccgacgaggt gaggaccttc tccaccaggt ccgagaaggt gaggaacatg     2400

gccaagaacg ccatcaagag gaacgccagg aagctggtga acttctacgt gggcaagggc     2460

atcaggacca tcgtggccga ggacaccgac ccgaccaagt ccaggaacga cggcaagaag     2520

tccaacagga tcaaggccgc ctggtccccg aagcagttcc tggccgccgt gaagaacgcc     2580

gcccagtggc acggcctgga gatcgccgag gtggacccga ggatgacctc ccaggtgcac     2640

ccggagaccg gcctgatcgg ctacagggac ggcgacaccc tgcactgccc ggacggctcc     2700

aagatcgacg ccgacgtggc cggcgccgcc aacgtgtgca gggtgttcgc cggcaggggc     2760

ctgtggaggt tctccatcaa caccaacatc gacatctcca acaaggacga gaagaagagg     2820

ctgagggcct acatcgtgca ccacttcggc tccgagtcca actgggagaa gttcaggaag     2880

cagtacccgt ccggcaccac cctgtacctg cacggcaggg agtggctgac cgccgaggag     2940

cacaagtccg ccatcgacag gatcagggac gacgtgggca gggacgccga gaacgaccac     3000

gtggccatcg tgaccgccgc cgagaaggtg gagatcttct ga                        3042


<210>  31
<211>  3159
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.13

<400>  31
atgtcccacg acctgaagcc gcagaggctg atcaggtcca acatcaccaa gacccactcc       60

gaccagaacg ccaagcaggt ggccgaggag gtgaagaagg agcacctgaa ctacctgctg      120

atcaagaacg agatgctgat ctccatcgtg ccggaggcca aggacgacga cggcaacgac      180

atcgacttca agaagcagct gaagtccctg tacaaggaga ccgaccagtc cgtgtccttc      240

tccgtgttct gccagatgat gaagttcagg aacatcgccc tgctgtacgc caagggccag      300

tccaggtggg ccgtgtcctc ctacttcacc ggcaacagga ggaaggacga ctacgccaag      360

gacctgtccc tgctggacga ggccatcgag ctgctggagt gcaagaggag gaagaaggcc      420

gaggaggaga acgaggagga gaacgagacc ccgaagaaga aggaggacaa cccgtccaac      480

atctccgagg agcagatcat gaagctgttc tacgccgtga acaagaagct gaaggagatc      540

ggctacctgg acaggtactc ccacatcgag aagcaggagc agtacgccat catcggcgtg      600

acctccagga ccgtgaaggc ctgggactac gccaacttcg ccaccaggaa ccactaccag      660

tccgtgcaga acgagtacca gaagaagctg aaggccctgc cgggcaccaa gaaggacaag      720

gtgtgcctgg agaagttctt cgaccacctg aacgagaaca acatcgccgc cgactgggac      780

aagtggaggc tgaagaagca catcctgcag tgcatcatcc cggccgccaa gatcggcctg      840

aaggagctga agcagtcctt ctacgtggac aacaagggca acaagcacaa ctacttcgtg      900

aacggcctgt acgaggagat cctgaagagg ccgttcctgt actccgccga ggacccggag      960

gagtccatcc tgtacctggg cgtggaggtg gcctccctgc actccaagct gaaccacctg     1020

aggtccgagg ccaggttctc cttcgagacc ccggacgaca tctgcaagta catgaccatc     1080

tgcggcgaca actaccacaa cttcaccatg tccgccatcg gcgaggacgt ggaggacatc     1140

gaggtggagg tgtacgacta caaccactcc aagaagtacg agaccatgag gttcatcaac     1200

ggcaagagga ccaccgacct gtccctgaac ttcaagggca tcccggtgag gctgtgcctg     1260

gagggcaaga ggaacaactc ctacttcgcc gacgccatcg tgtgggagct ggacaacaag     1320

gacaagaccg gctacctgat cgagtacggc aagtccaaca acaggctgta catgctggtg     1380

aaggagccgc tgatcggctg caggaggaag ttcggcaagg acgtgctgtt cgtgtccctg     1440

tccggcaccc tggtgaacaa gtacatcgag gacgacatcg tgtccgccag gtacctgatg     1500

cagaccgccg ccccgatctt caagacctcc agggccaaga agcaggacaa gatcggcgac     1560

aagtggttcg agcactgcca gggctccacc atcaagatcg ccggcatcga catcggcatc     1620

aacccgatcg ccgccatcac cgtggccaac gtgaccttcg acagggccct gggcaacaag     1680

atcaagaacc agaagcagat cgtgatcgac tgctacgccg aggactacaa gatcgacccg     1740

gtggtggtga agaggatgga ggacatcagg cacatcaagt acaccatcaa ctcctggtac     1800

cacctggccg actgctgcag gctgaaggcc gccaacaagg agtacgtggt gaacgagagg     1860

aagcagggct tcttcaggga gaacatcgag tacctgaagg aggtggccaa gaaggccatc     1920

accgagtccg accagcagat caaggagcag aaggccgccc tgaagaggtt cgacggcgag     1980

aagaagaagg agatccaggc caccatcaac ggcttcaacc tgaagatcaa gatcctgaag     2040

aagttcgtga ggcagtccgc caagaagatc ttcgactcca ccctggagac cctggagaag     2100

tacgacaaca acatcgagca ggccaagagg gacagggagt tcggcctgaa gatcatctac     2160

gacctgatca tcaagtacta caagaggtcc aagaaggaga gggagatgaa ccagaggatc     2220

tacgtggacg actacaacca ggaggagatc gacaccgaga ggaccaagaa gatcaggaag     2280

gagaccatca ccttctgcga caacgactgg aactccctga ccaagaggat ccacgacctg     2340

gagaagaaga tgaagaagat cggcatctcc gagccgggca gggtggagca ggagatcaac     2400

gacagggact actacaacaa catccaggac aacaccaaga agaggcaggc caagatcatc     2460

gtggacgccc tgaaggagga gggcgtgtcc atcatcgtgg tggaggacct gaccggcggc     2520

ggctccgaga acaccaagga gatcaacaag tccttcgacg ccttcgcccc gatcaggttc     2580

ctgaacgccc tgaagaactg cgccgagacc aacggcatcc aggtgaccga ggtgctgtcc     2640

ccgatgtcct ccaagatggt gccgtccacc ggcgagatcg gccacaggga caagagggac     2700

aagcagctgt actacaagga cggcgaggag ctgaagtcca tcgacggcga catctccgcc     2760

tccgagatcc tgctgaggag gggcgtgtcc aggcacaccg agctgatcgg caccatgaac     2820

gtggaggacg tgctggacaa gaacaacaac aagaacaagt gcatcaaggg ctacgtgtgc     2880

aacaggtggg gcaacatcca gaacttcgag aagatcctga aggagaaggg catcggcgag     2940

agggagatca tctacctgca cggcgacaag atcctgacca tggacgagaa gaggaccctg     3000

caggcctcca tcaggaagga gctgaaggag atgagggaga gggagtccgg cgaggagaac     3060

gccggcaccg ccaggaagaa gtccaagccg aagaagaaga agaagatcaa gaggaacaac     3120

gaccaggacc tgtccaacaa caggccggcc gcctcctga                            3159


<210>  32
<211>  3138
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.14

<400>  32
atgaaggaga acaagatgaa ggagaacggc tccatgacca cccactccaa ggtgatcgcc       60

ctgaagatga agtccgagaa cgtggagttc gacaccttct acaaggagtc cttcgagctg      120

ttcaagcagt tcaccaacga gttcgtggcc tggggcaacg acgagatcta ccagtacggc      180

tcctccaaga ggaagaagga cgaccagaag atctccctga tcccggtgat cgaggacatc      240

tacaagtccg tggagaagaa ggccaccgcc gagggcatct ccaagaccga cttcagggcc      300

gtgctgaagt acctgtacca ccagatcatc aacgtgggca actccggcag gtcctacggc      360

acctccctgt tcggcggctg cgaggtgaag gagaagctgt ccaagcagga catctccaac      420

atcgtggagt gcgtgaagga gctggagctg tgcaagtcca agcaggagga gtccgacgcc      480

tacgacaaga tcctgctgaa ggagaagatc acccacatcg tgaagtccgg cgagaccgcc      540

ggcgacatca ccaagaagta caaccaggcc accaccggca ggaagacctc ctccaagggc      600

ttcttcgaca agtccaccaa gaccgaggtg aagtacaagg acatcaagga cgacaccctg      660

ctgcaggacg gctccaccat cttcatcaag tcctccgtgg acctgttcgt gaagaaggtg      720

tgcaacaccc tgagggagat caacttcttc gacaggctgc cgttcaagaa caaccactcc      780

aacaactacg gcctgctgtt ctccatgctg tcccagatcg agtcctggaa gaccatctcc      840

gagaccacca agaagtccca cgaggagcac ggcgagaaga tcgcctccat ggtgaagaag      900

ctggacctga cccagaccga gctgatgaag gacttcgccg ccttctgcat cgagaacaac      960

atcaccaaga agttcgacca caagttcaag aggcacatgg aggactgcgt gatcccgtcc     1020

ttcaagaacg gcaagatccc ggacaagctg ttctacttca acatcatcct ggccaagaag     1080

accgacgagc agatcgacta ctccctgtcc tccgagttct acaccaagct gttctccatg     1140

ccgaacctgt ggcaggagga ggaggccttc atcgtgaaga acatcaacct gatcgaggag     1200

atcaccatct tcaacaagag gaggaactac gcctgctgcc cgctgatcaa ggagaaggag     1260

tacgacaggt tccagatcca gctgaacgag accaacttcc tgaagttcca gttcgacccg     1320

aagaacgtgg tgaacatcga cgagaacacc accgaggcca ccgtgggctt cgacgagaag     1380

ctgaagctgg tggtgtgcgc cgacaagaag tacgccttct ccatcttcac ccagtgcaag     1440

taccacggca acaagcacaa gccgaacacc tacttcaaca acctgaagat catcaaggtg     1500

atcgagtcca agtccaactc cgtgaagtcc atgaagtaca ccttcgagtt caccaagagg     1560

aacgagctga agagggccga gatcaagcag ccgtccatcg tgtacaagaa caacaactac     1620

tacatcagga tcaacatgaa cgtgatcctg gacgccgacc agacctccta caagatcatc     1680

aacaacaacc agaccgcctc cctgccgtcc tacttccagt cctccctgcc gttcgagaac     1740

aacaggggca agatccacga caagggcatc gtgcactggg agaagatcaa gaacaggaag     1800

atcatcgcca tgggcgtgga cctgggcgtg aggaggccgt tctcctacgc catcggcaac     1860

ttcaccctga acaaggacat cctggacaag aacgacgtga acatcgtggc ctccggcttc     1920

aacctgtgct ccgactccga cgtgtacttc caggtgttca accagatcaa gaccctggcc     1980

aagttcatcg gcaagctgaa gtcccacaac aagggcctga aggtggactt cgagaaggac     2040

aagaagtaca tcttcgacct ggtgaacgac gccaaggcct acttcaagga catgtccgcc     2100

aagaggatca acgacaccaa ggacaacatc tccaacaccg tgaccaacaa ggagaggatc     2160

tacggctcct tcgtgtccga gtccgccgag tccgccatcc agtgcgccat cgacaggtcc     2220

gagaaggagt ccggcctgac cctgaagaag gacatctcct ggctggtgaa cgtgctgtcc     2280

aagtacctgg agaggaagtt caaggaggtg aagaacaaca ggaagtacac caacgtgaac     2340

aagtgcgaca actgcttcaa ctggctgagg gtgatcgaga acatcaagag gctgaagagg     2400

tccatctcct acctgggcga ggacctgcag aagaacccgg agctgaagat cgagctgaag     2460

aacctgaacg agtacggcaa caacgtgaag tccgacttcc tgaagcagat cgcctccaac     2520

atcatcaagg tggccatcga gcacaagtgc gacatcgtgt tcatcgagaa gctgggcaag     2580

gccgactcca ggtccaggaa gctgaacgag atgttctcct tctggtcccc gaaggccatc     2640

aagaaggcca tcgagaacgc cgcctcctgg cacggcatcc cggtggtgga ggtggacccg     2700

tcctgcacct ccaaggtgca ctacgagacc aacctgttcg gccacaggat cggcaacgac     2760

ctgtactacg tggaggacca gtgcctgaag aaggtggacg ccgacatcaa cgccgccaag     2820

cagatcctgg tgaggggcgc caccaggcac ggcaacatct cctccatcaa catcaagtac     2880

ctgcaggcca agatcgccga gctgaactcc gaggccaact ccgaggagga caaggaggag     2940

atcaagcagg gcggcaagag gatccagggc ttcctgtgga agaagtacgg caacatcacc     3000

aacatcacca accagctgac cgccgcccac aaggagaggg agtccaagtt cgactacatc     3060

tacctgcaca acgacaagtg gatcgcctac gaggacagga acgagatcaa gaaggacatc     3120

gagaagaggc tggagtga                                                   3138


<210>  33
<211>  2688
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.15

<400>  33
atgaccgcca agaagaccgc caagaagtac ttcccgccga agtgcctgag gtcctcccac       60

ttcaagatct acggcatccc gaccgccatc agggccctgg aggagaccaa caccttcgtg      120

aacaaggccg ccgccgacct gatggagatg ttcttcctga tgaggggcca gccgtacagg      180

aggaggatcg gctccgagga gaagcaggtg acccaggagc acatcgacgc caggctgagg      240

gtgctggtgg gcgactactc cctgaacgag gtgaagccgc tgctgaggca gctgtacgac      300

ggcatcaagg ccaagcagaa ctacgccccg acccacttcg tgaggttctt catccagccg      360

accaagggcg ccatcgacaa gaagtccccg gtgtcccaga gggccaagaa ggccggccag      420

aagctgcaga agatgggcgt gctgccgatc ctgccgctgt ccccgggctt caagttctgg      480

accgccgcca tgatgatggc ctgctccagg atgaactcct gggaggcctg caacgagaag      540

accatcgaga accacaaggc cttcctggag ggcatcgaga actacaagaa ggagatcagg      600

ttcgaggacc tgtgcgagga gtggtccctg ttctccgact ggctgaccga ggccgagtcc      660

gacaacgagg gcggctgcaa gttcaagctg accccgaggt tcctgcagag gtgggagagg      720

atctacctga agcagatgag gaagggcaag atcccggcca ggcacaacct gggcccggtg      780

atggaggccc tggccggcga caagtacagg cagctgtggg acaacggcga ggagagggac      840

tacatcaccg agctgggcga cctggtgacc tcccagagga aggccgtgag gctgtccagg      900

gactccgccg tgaccttccc ggacgaggag ctgtccccgg tgggcaccga gttcggccac      960

aactacatgt ccttctccat cgaccaggag aactcccacc tggtgaccct ggaggtgatc     1020

ggcggcaagt accagttcga gatctccaag tccgactact tcagggacct gatcgtggag     1080

gaggccggca agcagtccaa gttctacaac gtgtcctaca ggaagggcaa cgtgagggag     1140

gagaacctgg ccggcgactt caaggaggcc accgtgagga acaggaggtc cctgaagacc     1200

ggcaagagga ggctgtactt ctacatgtcc cactccatcc cgaccaggtt cgacgacgac     1260

ctgtacgccc agttcaccga gaagggccag ccggacttct ccaagctgta caaggccgtg     1320

acctacttcc agtgctccct gggcaacaag aaggccgaca cctacagggt gtacgtgaag     1380

atgggcacca ggttcctggg cgtggacatc ggcgtgtcca ggctgttcgg cttctccctg     1440

ttcgagctga gggaggagaa gccggagaag aacccgttct tcgagctgcc ggacgacctg     1500

ggctacgccg tgtgcctgga gtcctgggtg gacggcgtgg agaagaacca caaggtggcc     1560

caggagatga aggactggag gagggagtgc ctggccgccc agaggctgat ccactacgcc     1620

aagttcctga agaagaggga caagaacgag gagatcgact acaagcacga ggagtccctg     1680

gagaccatcg ccggcctgct gggcatcgag atcgacccgg agcagatcat cgacgtgccg     1740

ctgaagctgc tggacctggt gggccaggcc atcggcgccc tgaggaagaa gtacctggtg     1800

ctgaagaaga acgaggtgag gcagggcagg atcacctccg agctgttcct gtggccggag     1860

tgcgtggaca cctacatcag gctgctgaag tcctggacct acaaggacaa gaagccgtac     1920

cagaagggcg agaccaacaa ggacgccttc aagaagctga agggctacct ggccaggctg     1980

aggaaggacc tggccccgaa gtacgccgcc gtgatcgccg acgccgccat caggcacaag     2040

gtgcacgtgg tggtggccga gaacctggag cagttcggcc tgtccatgaa gaacgagaag     2100

gacctgaaca gggtgctggc ccactggtcc caccagaaga tctggtccat ggtggaggag     2160

cagctgaggc cgtacggcat catggtggtg tacgtggacc cgaggcacac ctccaagctg     2220

gacttcgcca ccgacgagtt cggcggcagg tgcttcacct ccctgtacgt gatgagggac     2280

ggcaagaaga ccaccaccga caccgagaag aacgcctccc agaacatccc gaagaagttc     2340

ctgaccaggc acaggaacgt gtcctggctg ctggcctacg ccgtggacct gtccgactcc     2400

cagaagaaga agctgggcat cggcgacgag aaggtgtggc tgccgaacat gggcctgatg     2460

atctccggcg ccctgaaggc caagcacggc aagaactccg ccctgctggt ggaggacggc     2520

gagaactaca ggctgctgcc gatcaccgcc gcccaggcca agaagttcgt ggtgaagagg     2580

aagaaggagg agttctacag gcacggcgag atctggctga ccaaggaggc ccacaaggcc     2640

aggatcgagt acctgttccc ggagtccaag aagggcagga agtcctga                  2688


<210>  34
<211>  2871
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.16

<400>  34
atgaagaaga ccaactacaa gacctcccac ctgctgatcg acaacccgcc gcagtccatc       60

atcgacctgc acagggacgt gatcgagatc ggctcctacc tgaccaagtt cttcctggcc      120

tgcctgggca ggccggtgga ctccaccatc ctgtccgagc cggccctgca cttccagttc      180

gtgaacggca tcctgccggt gaagaacggc ccgggcgccg acgactcctc ctggaggcac      240

tccgagaact gctactccat gctgttcgag aagaactcca agtccggcaa gtccgacggc      300

aaggtgaggc aggtgaggga gctgaaggtg gccctgttcg gcaagaagga gaagggcaag      360

ggcatcgtgg gcaagaagac ctgggacgag ctgaaggtgg tgctggaggc cctgccggag      420

gagcaccaga tcctgtccct ggagatctgc cagaggcact acgagtccag ggacgtgaag      480

gccttcggca agctggccct gtcctccaag tccaggccgt ccgtggaggc cggcctgaag      540

ctgagggagc tgggcctgct gccgctggac tccaggggcc tggacaagaa caagctgctg      600

ggcatcctgg ccgccgtgac cggcaggctg aagtcctgga gggacaggga ctgcgcctgc      660

aaggccgaca agcaggccct gagggtgaag ttcgaggaga ggctgtccaa ggtggaccag      720

tccgcctacc agcagttcaa gcagttcgcc gacgagctgc tgacccagga gggctacagg      780

atctccggca gggtgctgag ggccgtggag aagaaggact ccgactactc cccggtgctg      840

accgtgctgg ccaagtaccc ggacctgcag gacaacttcg aggagctgtg cagggcctgc      900

ctggccgagc aggccttcaa caagaagaag gccgacgcca gggtgaccgt gtgctccgag      960

acctccccgc tgcagttccc gttcggcatg accggcaacg gctacccgtt caccctgtcc     1020

gcctgcgagg gcaggatcaa cgccaccatc cacttcccgg gcggcgacct gccgctgagg     1080

ctgaggaagt ccaagtactt ccagaacccg gagatcctgc cggtgaagga cggcttccag     1140

atcaccttca ccaggggcaa gaccccgctg gtgggcacca tcaaggagcc gtccctgctg     1200

aagaagaaca accactacta cctgtccctg agggtgaacg tgccgtccgt gaagatcccg     1260

aaggaggtga gggacaccag ggcctactac tcctccgccg tgggcggcga cgagaccacc     1320

ccggtgccgg tgaaggccgt ggccatcgac ctgggcgtga ccaccctggc cgactactcc     1380

atcatcgaca cctgcctgcc gggcgactgc aaggtgttcg gcggcgagac cgccgccttc     1440

accgcccacg gcaagatcgg ccagtgcgcc aacaagtccc tgagggacag gctgtacaag     1500

aacaccgagg aggccctgtt cctgggcaag ttcatcaggc tgtccaagaa gctgagggac     1560

ggcgagggcc tgaacaggtg ggaggtggag aagctgccgg gctacgccga gaggctgggc     1620

atcacccagc acctggacaa cgcctacacc aggaaggacg agatcgccag gaagttcaag     1680

cagatcaagg gcaacttcga caagctggtg tccgagttcg ccctgaggga ccacccgtcc     1740

aagaagggcg agtcctggga gaccatctcc gccgagacca tccaggtgct ggccgccctg     1800

aagaggatcc agtccctgct gaagtcctgg acctactact cctggaccgc cgaggactac     1860

gtgctggccc tgaccgccga cggcccggtg tgcatcgacg gcgagcacgt gaaggccgtg     1920

accgccacct ccaggaggtc cttcgccccg tgcggcaagg ccgccctgct gaggctgatc     1980

gagtccggcg agatcgtgga gaccggcggc cagtaccagc tggccaccgg cgtgaagcac     2040

aggaaccacc cggtgaactt cctgtcctcc tacatcaagc acttcaacgg cctgaggagg     2100

gacctgacca acaagctggt gagggccatc gtgaacaagg cccaggagta cagggtgcag     2160

atcgtgatcg tggaggactt cggcatcgcc gacctggagg acaggatcaa ggacgcctac     2220

gagaactaca ggtggaacct gttcgccccg gccaccatcg tgaagaagct ggaggccgcc     2280

ctgctggagg tgggcatcgc catggcccag gtggacccga ggcacacctc ccagatcgcc     2340

ccgaccggcg ccttcggctt cagggaccac gccttcctgt actaccagga cgacggcctg     2400

tgcaggatcg acgccaacac caacgcctcc atgaggatcg ccgagaggtt cttcatgagg     2460

cactccgtgc tgacccagct gagggccgcc aagatcggcg agaccgagta cctgatcccg     2520

gagtccgcct ccaagaggct gaacgccttc gtgaagctgc agaccggcaa gccgttcgcc     2580

aagctgatca tgaactgctc cggcttcgtg ctggagggcc tgaccaagaa gcagtacgcc     2640

aagctggccg agaccgccgg caagaaggag tccttctacc agtacgacga caggtggttc     2700

gacaagggcc accacttcgc ctgcagggcc accctggaga acaaggtgca ggtgtgcctg     2760

aacggcggcg gcaggatcaa ggacaccacc ccggacttca acccgaagtc cctgctgagg     2820

tccgacctgc agaccccgct ggaccagctg ttcggcaact ccggcgcctg a              2871


<210>  35
<211>  2841
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.17

<400>  35
atgtccaaca ccacctacaa gacctcccac ctgctgatcg acctgccgca gcaggagctg       60

atcgacctgc acagggactc caacgagatg ggctcctacc tgaccaagtt cttcctggcc      120

gccctgggca ggccggtgga caactccatc gtgctgccgc cggagctggc cgacctgtac      180

ttccagttcg ccaacggcat cctgccggtg gacaagggcc cgggctccga cgacccgtcc      240

tggctgcact ccgagaactg ctactccatg ttcttcgaga aggactccat gtccggcaac      300

tgcaccaaca agatcaagca gtaccaggag ctgaagaccg ccctgtgcgg ccagaaggtg      360

aagggccaga agggcctggt gggcaagaag acctgggccc agctgaagaa ggtgctgacc      420

gccctgccgc agaagtacca gatcctgtcc ccgaagatct gccagaagta cttcaagtcc      480

ggcaacctgg agggcttcgg caagctggcc ctggccggca agaacaggcc gtccatgtcc      540

gccggcctgc agctgaggga gctgggcctg ctgccgctgg actccagggg catcgacaag      600

aacaagctgc tgggcatcct ggtgggcatc accggcaggc tgaagtcctg gagggacagg      660

gactgggcct gcaagaccgt gaaggaggag ctgagggtga ccttcgagaa gggcctgggc      720

gaggtggacc cgaccgccta cccgcagttc aagcagttcg ccgaccagct gttcaagcag      780

gagggctaca agatctccgg cagggtgctg agggccgtgg agggcaagga cgccgactac      840

cagccggtgc tgtccctgct gacccagtac ccggacctgc agggcgactt cgaggagctg      900

ggcagggtgt acctggccga ggccgagtac ctgaggaaga aggtggacgc cagggtgacc      960

gtgtgcgacg ccgagacctc cccgctgcag ttcccgttcg gcctgaccgg caacggctac     1020

tccatcaccc tgaccgtggt gaagggccag atcgccgcca ccctgcacct gccgggcggc     1080

gacatcaccc cgaggctgag gaggtccaag tacttccaga acccggagat cgccccggtg     1140

aaggacggca agggcaaggt gaacggcttc cagatctcct tcaagagggg caagaccccg     1200

ctggtgggca tcatcaagga gccgaagctg ctgaagaaga acggcaacta ctacctgtcc     1260

ctggccgtgg gcatcaacaa gaccgagatc ccgaaggaga tctgcgacgc cagggcctac     1320

tactcctcca cctccaggac cgacaccccg ccggccgtga aggccatgtc catcgacctg     1380

ggcgtgacca ccctggccga ctactccatc atcgacaccg gcctgccggg cgactgcggc     1440

gtgttcggcg gctccaccgc cgccttcacc gagcacggca agatcggcag gtgcggctcc     1500

aagtccctga gggacggcct gtacaagaac accgaggccg gctacttcct ggccaagtac     1560

atcaggctgt ccaagaacct gaggggcggc gtgggcctga acaagctgga gaaggagaag     1620

ctgctggagc acgtggagag gctgggcatc gagcactgcg ccgacgactt cgccaggaag     1680

gacgagatcc acaggaagtt ctccgagatc aagtccaagc tggagaagtc catctccgag     1740

ttcgccctga gggacaggcc ggacaagaag ggcgcctcct gggagggcat ctgcgccgag     1800

accgtgcagg tgctgggcgc cgtgaagagg tggcagtccc tggccaagtc ctggacctac     1860

tactcctgga ccgccgagga ctacgtgctg gccctgaccg gcgagggcag gaccagggtg     1920

tccgacgagc acgtggagtc cgtggtgaag accggcagga ggcagttcgc cccgtgcggc     1980

aaggccgccc tgctgaggct gctggagaag ggcaagatcg tggaggtgtg cccgggccag     2040

ttccagctgg ccgagggcgt ggactacaag aggcacccga ccgagttcct ggccgcccac     2100

atcaggcact tcaacggcct gaggagggac ctgaccaaca agctggtgag ggccatcgtg     2160

gagaaggccc agcagcacag ggtgcagatc gtgatcgtgg aggacttcgg catcccggac     2220

atcgagggca ggatcatgga ccactacgac aactacaggt ggaacctgtt cgccccggcc     2280

aaggtgatcg agaagctgga ggaggccctg tccgaggtgg gcatcgccat ggccgaggtg     2340

gacccgaggc acacctccca gctggccccg accggcgact tcggcttcag ggaccacgag     2400

aacctgtact tctgggagaa gggcctgtgc aggaccgacg ccaacaccaa cgcctccatg     2460

aggatcgccg agaggttctt caccaggcac tccgtgctgt cccagctgag ggccgtgaag     2520

atctccgaga ccgagttcct gatcccggtg tccaccggca agagggagaa cgccttcatc     2580

aagtcccaga ccggcaagct gttcgccaag ctggtggccg actccaacgg cttcgtgatg     2640

gtgggcctga ccgagaagca gcacggcgcc accgtgaccg tgggcaagaa ggtgtccttc     2700

tacaaccacg ccggcaggtg gctgggcaag gcccaccaca tcgcccacag ggacaggatc     2760

aagaacgagg tgaaccaggt gctgacctcc ggcaggggca ggatcaggaa catcgccccg     2820

gagctgtccc cgaagacctg a                                               2841


<210>  36
<211>  2793
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.18

<400>  36
atgaccaacc agaagccgaa gttcaagtcc tccgacatcc agatcaagca catctccccg       60

accgacaaga agaggctgaa gaccttctac caccagctgt acgagcaggt gaacttcatc      120

ctggagagga tgatcgtgat gaggggcagg ccgaggacca tcaggaacat cgacggcacc      180

gagatcttcg tgtcccagga ggaggccgac cagcagctgc tgtccctggc cggcggctcc      240

cacgagggcg tgaagtacct gaagcagtac tacgagtcct gcgtggacgc cggcaagccg      300

gccaagtacg ccgccaacat gttcctgacc aagaccatct ccggcaccaa cccgctgcag      360

tgccacaccg ccgtgtacaa gctgtacaag aaggtgcagg ccaagcagat caccaagaag      420

gagttcatcg acaagctgta ctccaagacc aagaagaaga agtccctgaa gccggcctac      480

aaggtgttca ccgagaacga gcacatcgag ttctaccaca aggtgaggtc cggcaagctg      540

ccggcctccg aggtgaggct ggaggagtcc aggagggccc cggacgtggg cctggaggtg      600

ggcctgctgc tgagggagct gggcatcttc ccgttcaact tcccgcactt caccgagaag      660

aagtacctgg acctggcctg gaccatcgcc atcaggtggc tgaagaactg gaacgagaac      720

aacaagaaca ccgccaagga gaaggccaag cagaaggcca tcgtggacaa gctgaggacc      780

tccctggacc agaaggaggt ggacctgttc gaggagttcg ccgaggagtg ctcccaggag      840

cagttcggca tcagggaggg cttcgtgaag gccaagaaga ggctgaagtc cttcccgaag      900

ggcatcgaga agtcctccta caaggagggc atgaggatcc tggtgcagaa caagcacggc      960

tccatctggg acaacttcga gaacctggcc taccaccaca tcgccctgaa cgagtacaac     1020

aggctgaggg acgaggcctc cttctccttc ccggacccga tctaccaccc gatcagggcc     1080

gagttcggcc tgacctccct gccgaagttc aacgtgggcc tgaacgacag gggcaactac     1140

gagttcacca tcaacctgcc ggacggcccg ctgatgatgc tgggcaagaa gtccaggtac     1200

tacctgaagc cgatcatcca gggcccgctg aacaacgcct tctccttcga gttcatcaag     1260

ggcaacaaga agaggccgaa gatctccgcc aagctgaagt ccatcaccgt ggtgttcgcc     1320

aagtcctcca tctacgtggg cctgccgtac aggccgatct ccatcccgat cccgcaggcc     1380

gtgaccaact ccacctacta cttcaagaag aacctgtcct ccacctccaa gttcgacaag     1440

gacgtgttca tgggcctgac cgccgtgtcc gtggacctgg gcctgaaccc ggtgttctcc     1500

atgtccgcct gcaggctgga cgagatgaag gccgacgagc actactcctg cgaggtgccg     1560

ggcttcggct gggccaacca gatctggtcc aagagggccg gcggcgtgtg gaacaggtcc     1620

ttcagggaca agatcagggg cttcgtgccg ggcaacctgt ccgacaggat cttctgctgc     1680

aagaagtcca tcatcgtgtc caagaagctg agggacgaga agccgctgac ccagtacgag     1740

gaggagaact tcgagaggtg gatgcaggtg gtgggcgtgg acccgaacga ggaccactac     1800

aagcagctga ggatcgccat cagggacatc aagaccgagt acgagaccgt gaggtccgag     1860

ttcgccctga gggaccaccc gaacaactcc aacaagacca ccgagaacat ctgcaccgag     1920

tgcttcgaca tgctgttcgt gatcaagaac ctgatctccc tgctgaagtc ctggaacagg     1980

tggcacagga ccaccggcga catcgaggag aggggcaagg acccgaacga gtgctccacc     2040

tactggaggc actacaacgg cctgaagacc gacctgctga agaagctgac caacatcctg     2100

atcgagtccg ccaagtccat cggcgcccac atcatcatcc tggaggacct gaccctgtcc     2160

cagaggtcct ccaggtccag gagggagaac tccctggtgg ccatcttcgg cgcccagacc     2220

atcatcaaga ccatctccga ggaggccgag atcaacggca tcctggtgta cctggaggac     2280

ccgaggcact cctcccagat ctccatcgtg accaacgagt tcggctacag gccgaaggag     2340

gacaaggcca agctgtactt catggacgag gagaccgtgt gcgtgaccaa ctgcgacgac     2400

tccgccgccc tgatgctgca gcagtccttc tggtccaggc acaaggacgt ggtgaaggtg     2460

aagggcacca aggtgtccga caccgagtac ctggtgtcct ccgaggacaa ggacggcacc     2520

aagatgaggc tgaggtccta cctgaagagg aacgtgggca ccgccaacgc catcctgcag     2580

aagaactgcg acggctacga cctgaagaag atctccccgc agaagaagaa gaagatcgag     2640

gagttcggca aggacgagta cttctacagg cacggcgagc agtggttcac cgccgacgcc     2700

cacttcgaca agctgaggga gttcggcaac caggtgttcc tgaccccgca gtcccagatc     2760

aagaggatca acctgcaggt ggagggcacc tga                                  2793


<210>  37
<211>  2727
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.19

<400>  37
atgccgtcct acaagtcctc cagggtgctg gtgagggacg tgccggagga gctggtggac       60

cactacgaga ggtcccacag ggtggccgcc ttcttcatga ggctgctgct ggccatgagg      120

agggagccgt actccctgag gatgagggac ggcaccgaga gggaggtgga cctggacgag      180

accgacgact tcctgaggtc cgccggctgc gaggagccgg acgccgtgtc cgacgacctg      240

aggtccttcg ccctggccgt gctgcaccag gacaacccga agaagagggc cttcctggag      300

tccgagaact gcgtgtccat cctgtgcctg gagaagtccg cctccggcac caggtactac      360

aagaggccgg gctaccagct gctgaagaag gccatcgagg aggagtgggg ctgggacaag      420

ttcgaggcct ccctgctgga cgagaggacc ggcgaggtgg ccgagaagtt cgccgccctg      480

tccatggagg actggaggag gttcttcgcc gccagggacc cggacgacct gggcagggag      540

ctgctgaaga ccgacaccag ggagggcatg gccgccgccc tgaggctgag ggagaggggc      600

gtgttcccgg tgtccgtgcc ggagcacctg gacctggact ccctgaaggc cgccatggcc      660

tccgccgccg agaggctgaa gtcctggctg gcctgcaacc agagggccgt ggacgagaag      720

tccgagctga ggaagaggtt cgaggaggcc ctggacggcg tggacccgga gaagtacgcc      780

ctgttcgaga agttcgccgc cgagctgcag caggccgact acaacgtgac caagaagctg      840

gtgctggccg tgtccgccaa gttcccggcc accgagccgt ccgagttcaa gaggggcgtg      900

gagatcctga aggaggacgg ctacaagccg ctgtgggagg acttcaggga gctgggcttc      960

gtgtacctgg ccgagaggaa gtgggagagg aggaggggcg gcgccgccgt gaccctgtgc     1020

gacgccgacg actccccgat caaggtgagg ttcggcctga ccggcagggg caggaagttc     1080

gtgctgtccg ccgccggctc caggttcctg atcaccgtga agctgccgtg cggcgacgtg     1140

ggcctgaccg ccgtgccgtc caggtacttc tggaacccgt ccgtgggcag gaccacctcc     1200

aactccttca ggatcgagtt caccaagagg accaccgaga acaggaggta cgtgggcgag     1260

gtgaaggaga tcggcctggt gaggcagagg ggcaggtact acttcttcat cgactacaac     1320

ttcgacccgg aggaggtgtc cgacgagacc aaggtgggca gggccttctt cagggccccg     1380

ctgaacgagt ccaggccgaa gccgaaggac aagctgaccg tgatgggcat cgacctgggc     1440

atcaacccgg ccttcgcctt cgccgtgtgc accctgggcg agtgccagga cggcatcagg     1500

tccccggtgg ccaagatgga ggacgtgtcc ttcgactcca ccggcctgag gggcggcatc     1560

ggctcccaga agctgcacag ggagatgcac aacctgtccg acaggtgctt ctacggcgcc     1620

aggtacatca ggctgtccaa gaagctgagg gacaggggcg ccctgaacga catcgaggcc     1680

aggctgctgg aggagaagta catcccgggc ttcaggatcg tgcacatcga ggacgccgac     1740

gagaggagga ggaccgtggg caggaccgtg aaggagatca agcaggagta caagaggatc     1800

aggcaccagt tctacctgag gtaccacacc tccaagaggg acaggaccga gctgatctcc     1860

gccgagtact tcaggatgct gttcctggtg aagaacctga ggaacctgct gaagtcctgg     1920

aacaggtacc actggaccac cggcgacagg gagaggaggg gcggcaaccc ggacgagctg     1980

aagtcctacg tgaggtacta caacaacctg aggatggaca ccctgaagaa gctgacctgc     2040

gccatcgtga ggaccgccaa ggagcacggc gccaccctgg tggccatgga gaacatccag     2100

agggtggaca gggacgacga ggtgaagagg aggaaggaga actccctgct gtccctgtgg     2160

gccccgggca tggtgctgga gagggtggag caggagctga agaacgaggg catcctggcc     2220

tgggaggtgg acccgaggca cacctcccag acctcctgca tcaccgacga gttcggctac     2280

aggtccctgg tggccaagga caccttctac ttcgagcagg acaggaagat ccacaggatc     2340

gacgccgacg tgaacgccgc catcaacatc gccaggaggt tcctgaccag gtacaggtcc     2400

ctgacccagc tgtgggcctc cctgctggac gacggcaggt acctggtgaa cgtgaccagg     2460

cagcacgaga gggcctacct ggagctgcag accggcgccc cggccgccac cctgaacccg     2520

accgccgagg cctcctacga gctggtgggc ctgtccccgg aggaggagga gctggcccag     2580

accaggatca agaggaagaa gagggagccg ttctacaggc acgagggcgt gtggctgacc     2640

agggagaagc acagggagca ggtgcacgag ctgaggaacc aggtgctggc cctgggcaac     2700

gccaagatcc cggagatcag gacctga                                         2727


<210>  38
<211>  2466
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.20

<400>  38
atggccttcc agtccaagag gaggatcgtg ggcaacttcg tgaaggagca gtgcctgaag       60

gccgtggacg gcaaggtgat cctgaccgac caggagaaga gggagctgat caagaggtac      120

gagctgcacc tggagccgca caagtggctg ctgaggctgt tcctgtccgg ctacgagggc      180

agggacgacg gcttctacga ggagctgggc aacaccaacc tggacaagga gaagttcttc      240

gaggtgaccg ccggcctgag ggacgccctg ctgaggcagt ccggctcctc cagggccctg      300

aagtcctcca tgctgggcaa gtgcccgccg tccgccgccg tgggcaaggc cgccaagcac      360

atccagaccc tgagggacgc cggcatcctg ccgttcaaga ccggcctgac ctccggcgag      420

gactacaacg tgctgcagca ggccgtgcag cagctgaggt cctgggtggc ctgcgaccac      480

aggaccaggg aggcctacgc cgagcagcag gagaagacct cccaggccga ggaggccgcc      540

aagaaggccg ccaacgaggt gaagccggag gacgccaagt ccctggagag gcacgagagg      600

gtgctgacca agctgaggaa gcaggagagg aggctggaga ggatgaagtc ccacgcccag      660

ttctccctgg acgagatgga ctgcaccggc tactccctgt gcatgggcgc caactacctg      720

aaggactact gcctggagaa ggagggcagg ggcctgaggc tgaccctgaa gaactccacc      780

atggccggct cctactacgt gtccgtgggc gacggccagc acgccggcat gaagaacccg      840

ggcaccccgg ccggcggctc cccggagaag ggcaggagga ggaacatcct gttcgacttc      900

accgtggaga agtgcggcga caactacctg ttcaggtacg acgagaacgg caagaggccg      960

agggccggcg tggtgaagga gccgaggttc tgctggagga ggaagggcaa ctccgtggag     1020

ctgtacctgg ccatgccgat caacatcgag aactccatga ggaacatctt cgtgggcaag     1080

cagaagtccg gcaagcactc cgccttcacc aggcagtggc cgaaggaggt ggagggcctg     1140

gacgagctga gggacgccgt ggtgctgggc gtggacatcg gcatcaacag ggccgccttc     1200

tgcgccgccc tgaagacctc caggttcgag aacggcctgc cggccgacgt gcaggtgatg     1260

gacaccacct gcgacgccct gaccgagaag ggccaggagt acaggcagct gaggaaggac     1320

gccacctgcc tggcctggct gatcaggacc accaggaggt tcaaggccga cccgggcaac     1380

aagcacaacc agatcaagga gaaggacgtg gagaggttcg actccgccga cggcgcctac     1440

aggaggtaca tggacgccat cgccgagatg ccgtccgacc cgctgcaggt gtgggaggcc     1500

gccaggatca ccggctacgg cgagtgggcc aaggagatct tcgccaggtt caaccactac     1560

aagcacgagc acgcctgctg cgccgtgtcc ctgtccctgt ccgacaggct ggtgtggtgc     1620

aggctgatcg acaggatcct gtccctgaag aagtgcctgc acttcggcgg ctacgagtcc     1680

aagcacagga agggcttctg caagtccctg tacaggctga ggcacaacgc caggaacgac     1740

gtgaggaaga agctggccag gttcatcgtg gacgccgccg tggacgccgg cgcctccgtg     1800

atcgccatgg agaagctgcc gtcctccggc ggcaagcagt ccaaggacga caacaggatc     1860

tgggacctga tggccccgaa caccctggcc accaccgtgt gcctgatggc caaggtggag     1920

ggcatcggct tcgtgcaggt ggacccggag ttcacctccc agtgggtgtt cgagcagagg     1980

gtgatcggcg acagggaggg caggatcgtg tcctgcctgg acgccgaggg cgtgaggagg     2040

gactacgacg ccgacgagaa cgccgccaag aacatcgcct ggctggccct gaccagggag     2100

gccgagccgt tctgcatggc cttcgagaag aggaacggcg tggtggagcc gaagggcctg     2160

aggttcgaca tcccggagga gccgaccagg gagcaggacg agtccgacca ggacttcaag     2220

aagaggctgg aggagaggga caagctgatc gagaggctgc aggccaaggc cgacaggatg     2280

caggccatcg tgcagaggct gttcggcgac aggaggccgt gggacgcctt cgccgacagg     2340

atcccggagg gcaagtccaa gaggctgttc aggcacaggg acggcctggt gctgaacaag     2400

ccgttcaagg gcctgtgcgg ctccgagaac tccgagcaga aggcctccgc caggaactcc     2460

aggtga                                                                2466


<210>  39
<211>  2514
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.21

<400>  39
atgggcaggt tcggcaagaa gaagatcgcc gtgaacggct acgtggagca ggactgcatc       60

aagaccatct ccgccaagtg cctgctgacc agggcccaga tcgacgagct gagggccaag      120

tacgacgccg tgctggacac catgaggccg ctgatcaggc tgatcctggc cggctacgag      180

ggcagggacg acggcatcta cgaggagatc gccccggaga tgtccaagaa gaagttcttc      240

gaggccgcca ccgagtggag ggagtccatc gtgaagaacg cctccccgag ggccatgaag      300

gcctccgtgt tcggcgacaa ggagccgtgc aagtccaccg gcggcgccag ggccgtgatc      360

ggcaagctga ggaagtccgg cgtgttcccg atcgagaccg gcctgtccgg cggcgacgag      420

tacaacctga tcgagcaggc catcgagtac gccaagtcct ggctgaagtc cgacgaggcc      480

accagggagg cctacgccga ccagcagaag gacatcaaga ggctgatcgg cgaggccaag      540

aagctggccc tgaagatcga gaaggccgag aagaagctgg aggccaccaa cccgcagacc      600

aagtcctgga agaagaccac cgagatcatc aagaagtcca agagggagtt cggctccgtg      660

accaccaaga ccgagaaggc cgagaagagg ttcgagagga tgaagccgtt ctccaagctg      720

gagctgcaga acatggactg caccaagtac tccacctacc tgggcaccaa ctactccccg      780

ttcaagctga agaaggaggg cgacctgctg cagatcaccg tgacctcctc cgtgatgaag      840

ggcacctacc tggcctccta cggcgacggc cagtacggct ccaggaggaa caacggccag      900

tccaggaggg acgacttcgt gccgaacatg aaccagaaga ggaggaggaa cctgatgttc      960

gactgcaccg tggagccgtt cggcgacggc tccctgctga ggtacgagga gaacggcctg     1020

aggccgaggg tggccgagct gaaggagccg aggctgtgct ggaggaggag gaacggcaac     1080

tacgagctgt acctgatgat gccggtgaag atgcacgtga agtccccgga gatgttcgcc     1140

ggcgaccacc tggccttctc caggtactgg ccgaaggagg tggagggcct ggactccgac     1200

accaagatca ccgccctggg cgtggacgtg ggcatcatca ggtccgccta ctgcgtggcc     1260

gtgaccgccg agaggttcgt ggacggcctg ccgaccgaga tgaccgtggg caaggcctcc     1320

ttcgacgccc agaccgagaa gggcagggag tacttcgagc tgggcaggag ggccaccatg     1380

ctgggctggc tgatcaagac caccaggagg tacaagaagg acccgaagaa cgagcacaac     1440

cagatcaagg agtccgacgt ggccgccttc gacggctccc cgggcgcctt cgagcactac     1500

atcctggccg tggacgagat gtccgacgac ccgctggacg tgtggggcca cgccaacatc     1560

accggctacg gcaagtggac caagcagatc ttcaaggagt tcaaccagct gaagagggag     1620

agggccgagg gccaggtgga gccgaacatg accgacgacc tgacctggtg ctccctgatc     1680

gactacatca tctccctgaa gaagaccctg cacttcggcg gctacgagac caaggagagg     1740

gagtccttct gcccggccct gtacaacgag agggccaact gcagggacgt ggtgaggaag     1800

aggctggcca ggtacgtggt ggagagggcc atcgccgccg aggcccaggt gatctccgtg     1860

gagaacctgt ccaagtgcag gagggacgac aagaggaaga acagggtgtg ggacctgatg     1920

tcccagcagt cctggatcgg cgtgctgacc aacatggcca ggatggagaa catcgccgtg     1980

gtgtccgtga acccggacct gacctcccag tgggtggagc agtgcggcgc catcggcgac     2040

aggaaggcca ggaccatcgc ctgcagggac gtgaacggca agttcgtgtc cctggacgcc     2100

gacctgaacg ccgcctacaa catcgcctcc agggccctga ccaggcacgc cgagccgttc     2160

tccatcacct tcaagaagaa ggacggcatc ctggagcaga aggacgtgtg cttcgacccg     2220

ggcgtgatcc cggtgctgga gaagaacgag aacgaggaga agttcaggga gagggtggag     2280

aagtacgaga agtccctggt gatcaagcag gagagggccg tgaggtggag ggccatcctg     2340

cagcacctgt tcggcaacga gaggccgtgg gacgagttca ccgacgaggt gaaggagggc     2400

aggcacgtgt ccctgtacag gcaccacggc aagctggtga ggaccaagca gtacgccggc     2460

ctggtgaagg aggccaacaa cgagctggtg ccggtgtgcg ccgtggccag gtga           2514


<210>  40
<211>  2907
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.22

<400>  40
atgtccaagg ccaccaggaa gaccaagacc accgtgccgg agtccaccga caccgagtcc       60

ccggccgccg acacccaggt gagggtgcac tggctggccg cctcccacag ggcctccccg      120

ggcctgcagc aggtgaagga gatgatccag cagcacgccg acgtggcctc cgtgctgttc      180

cagggcctgg tgaggaccgc cccgatcgtg ttcaggaacg acgacggctc cccggtgaag      240

ccgctggacc tgctgctggc ctccctgagg ccgacctaca aggtgcagag ggacaccgag      300

accgtgctgg tgaccaagga cgacgtgatc aggtgcctga ccctggccac caccgccgtg      360

aacggcggcc aggccaccaa cgtggccgtg ttcgcctccg ccgacccggc cctgtccgcc      420

ccgctggcca ccctgctggc ccagctgagg gccctggagt ccgtggactc ctcctggtcc      480

gtggtgggca agctggacat caacctgagg aagttcgtgt ggctggtgct gtccgccgcc      540

ggcgtgctgc cggccctggc cgacctggag ggctacgccg ccaagtccgt gctggccaac      600

gtgcagggca agtacaagtc cctgcaggcc tgcgccgaca cccacgccgc cctgtacaag      660

cagcaccaga ccaacaagga gcagctggag aagctgatcg ccgacccggg cttcgtggcc      720

ctgtgctccg ccctgctgca ggacccggac ctgaggtccg tggactccag gaggctggcc      780

gccctggagg agatgctggg cttcgtggcc gccgacaaga actactccga gtacacctcc      840

accaggaagt gcgacggctg ggccccgccg gccaacatgt tcgacctgct gtgcgagcac      900

aaggaggccg tgaggaggaa catcgtggtg gacaactcca agtgcctgtc caggaggatc      960

tccctggtgg ccgacggcga cgtgaacgag gtgtccgtgt tcgagctgct gaacgagatg     1020

aggtggctgt ccgtgcactc ctccggcatc aggatgccga actacccgaa gcacgcctac     1080

gccctgaagt tcggcgacaa ctacatctcc gtgaagtcct tcgagaccgt ggtggacggc     1140

ggctgctccc tgctgaggat gaccgccagg gtgggcaaga acgacctggt gtgcgacttc     1200

gtgctgggca ggggcaacga gtactggaac aacctgaaga tcaccccgat gggcaagggc     1260

atcttcgccg tggtgaagac cgtgaggagg ttcaccgcca ccggcgccaa gctggtggag     1320

ctgaggggcg tgtgcaagga gccggagatc aggtacgaga ggggcgtgct gggcctgagg     1380

ctgccgatct ccttcgacgt gtacggcaag gtggaggagg actccatcgc cttcggcaag     1440

aacagggtgt ccctgaggac caccccgttc gtggagaagg ccgacaagtt ccagggcctg     1500

ctggactaca ggaacaccac cgccagggac ggctacatct actacgccgg cttcgaccag     1560

ggcgagaacg accaggtggt gggcatctac aggaccagga cctacaagaa cgccaccatg     1620

ctggagttct tcaacgtgtc cgacaccctg gaggaggtgg cctcctgcag gttctccgac     1680

taccaggaga ggaagaggag gctgaggggc gacaccggcg tgctggacat caactccatc     1740

aacgtgctgg ccgacaaggt gcagaggctg aggaggctga tctccaccct gagggcctgc     1800

gcctcccaca ccgactggta cccgaagctg aaggagagga ggaggctgga gtgggccgtg     1860

ctggcccagg gcgtgggcgt gtccgacttc gacaccgaga tcgagagggc cgagaccgcc     1920

ctgtccgccg tggccgccgt ggacttcgtg agggacccga cctgcatcat caacgtgatg     1980

gacaagcaca tctacgccca gttcaagcag ctgaggtccg agaggaacga gaagtacagg     2040

tcccagcacc agcacgacta caagtggctg cagctggtgg actccgtgat ctccctgagg     2100

aagtccatct acaggttcgg caaggccccg gagccgaggg gcgccggcga gctgtacccg     2160

cagaacctgt acacctacag ggacaacctg atgcagcagt acaggaagga ggtggccgcc     2220

ttcatcaggg acgtgtgcct ggagcacggc gtgaggcagc tggccgtgga ggccctgaac     2280

ccgacctcct acatcggcga ggactccgac gccaacagga agagggccct gttcgccccg     2340

tccgagctgc acaacgacat cgtgctggcc tgctccctgc actccatcgc cgtggtggcc     2400

gtggacgaga ccatgacctc cagggtggcc ccgaacaaca ggctgggctt caggtcccac     2460

ggcgactacc agaagttctc cgagaccgcc cagggcaggt tcaactggaa gcacctgcac     2520

tacttcggcg acaacgacgt gtccgagcac tgcgacgccg acgagaacgc ctgcaggaac     2580

atcgtgctga gggccctgac ctgcggcgcc tccaagccga ggttctccag gcagtccctg     2640

ctgggcaaga tcaagggccc ggtgctgagg acccagctgg cctacctggc ccacaagagg     2700

ggcctgctga ccgcctccac cgagccgaag aaggccgccg agaccggctt cgagctggtg     2760

gaggccgacc tgggcggcgc cctgagggtg ggcaagggct tcatctacgt ggacgccggc     2820

atctgcatca acgccaccac caggaaggag aggtcccaca aggtgggcga ggccgtggtg     2880

tccaggtccc tggcctcccc gttctga                                         2907


<210>  41
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.3ԭͬظ

<400>  41
ggugauauag uaacuggucu guuccagcac uucacc                                 36


<210>  42
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.4ԭͬظ

<400>  42
gugucaaugc gaugcugaac aucgcaugag uaacac                                 36


<210>  43
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.5ԭͬظ

<400>  43
gugcuggccg cucucgcuag agggagguca gagcac                                 36


<210>  44
<211>  37
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.6ԭͬظ

<400>  44
guugcaaucu aguagagaaa cuacagguaa uugcaac                                37


<210>  45
<211>  37
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.7ԭͬظ

<400>  45
auuacaaccu acugaugaua caguagguga uuguaac                                37


<210>  46
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.8ԭͬظ

<400>  46
gugcaauuaa guagaaauac ugcuagugau ugcaac                                 36


<210>  47
<211>  37
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.9ԭͬظ

<400>  47
ggugcaauca ucuggaaaua ccagagauaa uugcaac                                37


<210>  48
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.10ԭͬظ

<400>  48
gguacaggcu caagaaaaac uugagccaaa ugugac                                 36


<210>  49
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.11ԭͬظ

<400>  49
guuguaauac auuauguuaa aguaauguua uacaac                                 36


<210>  50
<211>  37
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.12ԭͬظ

<400>  50
gagguagugu ggaaguccag cagggcuucg uugacac                                37


<210>  51
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.13ԭͬظ

<400>  51
cuaucagugu aaaacccauc gaggguuuau cuacac                                 36


<210>  52
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.14ԭͬظ

<400>  52
auaucagugu ggguccgcaa aacggaucaa ugacac                                 36


<210>  53
<211>  37
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.15ԭͬظ

<400>  53
gugcagccua uugggaucgc ccauaggcau gagacac                                37


<210>  54
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.16ԭͬظ

<400>  54
gugccgucac cgccuuaguu gagcgggguc aagcac                                 36


<210>  55
<211>  37
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.17ԭͬظ

<400>  55
gugccaaccu caccggagac gaguggggca ccagcac                                37


<210>  56
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.18ԭͬظ

<400>  56
gugccgcugg ccuuucgaag aggggccuuu aagcac                                 36


<210>  57
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.19ԭͬظ

<400>  57
gugcugcugu cucccagacg ggaggcagaa cugcac                                 36


<210>  58
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.20ԭͬظ

<400>  58
guguaggccu ccucugaaug ggguggcuaa ugacac                                 36


<210>  59
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.21ԭͬظ

<400>  59
guguugaucc guucugaaug gauggauugc ugacac                                 36


<210>  60
<211>  36
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.22ԭͬظ

<400>  60
auuucagugc uggccugugg aagcaggcuc ugucac                                 36


<210>  61
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.3ԭͬظеı

<400>  61
ggtgatatag taactggtct gttccagcac ttcacc                                 36


<210>  62
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.4ԭͬظеı

<400>  62
gtgtcaatgc gatgctgaac atcgcatgag taacac                                 36


<210>  63
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.5ԭͬظеı

<400>  63
gtgctggccg ctctcgctag agggaggtca gagcac                                 36


<210>  64
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.6ԭͬظеı

<400>  64
gttgcaatct agtagagaaa ctacaggtaa ttgcaac                                37


<210>  65
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.7ԭͬظеı

<400>  65
attacaacct actgatgata cagtaggtga ttgtaac                                37


<210>  66
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.8ԭͬظеı

<400>  66
gtgcaattaa gtagaaatac tgctagtgat tgcaac                                 36


<210>  67
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.9ԭͬظеı

<400>  67
ggtgcaatca tctggaaata ccagagataa ttgcaac                                37


<210>  68
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.10ԭͬظеı

<400>  68
ggtacaggct caagaaaaac ttgagccaaa tgtgac                                 36


<210>  69
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.11ԭͬظеı

<400>  69
gttgtaatac attatgttaa agtaatgtta tacaac                                 36


<210>  70
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.12ԭͬظеı

<400>  70
gaggtagtgt ggaagtccag cagggcttcg ttgacac                                37


<210>  71
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.13ԭͬظеı

<400>  71
ctatcagtgt aaaacccatc gagggtttat ctacac                                 36


<210>  72
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.14ԭͬظеı

<400>  72
atatcagtgt gggtccgcaa aacggatcaa tgacac                                 36


<210>  73
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.15ԭͬظеı

<400>  73
gtgcagccta ttgggatcgc ccataggcat gagacac                                37


<210>  74
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.16ԭͬظеı

<400>  74
gtgccgtcac cgccttagtt gagcggggtc aagcac                                 36


<210>  75
<211>  37
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.17ԭͬظеı

<400>  75
gtgccaacct caccggagac gagtggggca ccagcac                                37


<210>  76
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.18ԭͬظеı

<400>  76
gtgccgctgg cctttcgaag aggggccttt aagcac                                 36


<210>  77
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.19ԭͬظеı

<400>  77
gtgctgctgt ctcccagacg ggaggcagaa ctgcac                                 36


<210>  78
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.20ԭͬظеı

<400>  78
gtgtaggcct cctctgaatg gggtggctaa tgacac                                 36


<210>  79
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.21ԭͬظеı

<400>  79
gtgttgatcc gttctgaatg gatggattgc tgacac                                 36


<210>  80
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.22ԭͬظеı

<400>  80
atttcagtgc tggcctgtgg aagcaggctc tgtcac                                 36


<210>  81
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  NLS

<400>  81

Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
1               5                   10      


<210>  82
<211>  1014
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.3-NLSںϵ׵İ

<400>  82

Met Thr Lys Glu Lys Ile Lys Lys Thr Lys Lys Ala Lys Val Glu Lys 
1               5                   10                  15      


Asp Ser Val Thr Arg Ala Gly Ile Leu Arg Ile Leu Leu Asn Pro Asp 
            20                  25                  30          


Gln His Gln Glu Leu Asp Thr Leu Ile Ser Asp His Gln Glu Ala Ala 
        35                  40                  45              


Arg Glu Ile Gln Thr Ala Thr Tyr Lys Leu Ser Gly Leu Lys Leu Tyr 
    50                  55                  60                  


Asp Lys Thr Asn Asn Met Val Val Asp Gly Ser Lys Ala Thr Pro Glu 
65                  70                  75                  80  


Glu Gln Glu Ala Tyr Tyr Lys Ile Ile Asn Trp Glu Gly Gln Pro Ile 
                85                  90                  95      


Ser Ile Ser Asn Pro Met Val Arg Ala Thr Phe Lys Ser Ile Ala Lys 
            100                 105                 110         


Val Lys Glu Asp Ile Arg Arg Lys Gln Glu Glu Tyr Ala Lys Leu Glu 
        115                 120                 125             


Glu Ala Asp Leu Thr Lys Met Ser Thr Gly Asp Val Lys Lys His Lys 
    130                 135                 140                 


Asn Glu Leu Arg Lys Ala Ala Asn Arg Ile Lys His Ser Glu Glu Ile 
145                 150                 155                 160 


Leu Gln Phe Ala Lys Trp Arg Leu Ala Asp Ile Phe Pro Leu Pro Leu 
                165                 170                 175     


Ser His Asn Ser Gln Leu His Leu Lys Asn Asn Tyr His Gln Asn Val 
            180                 185                 190         


Phe Ser Gly Phe His Ala Arg Val Lys Gly Trp Asn Ala Cys Asp Ile 
        195                 200                 205             


Ala Ala Gln Ala Asn Tyr Ala Glu Ile Asp Asn Arg Leu Thr Glu Leu 
    210                 215                 220                 


Ser Ser Glu Leu Ser Gly Asp Tyr Gly Ser Glu Val Ile Thr Asp Leu 
225                 230                 235                 240 


Met Gly Leu Leu Gln Tyr Thr Lys Glu Leu Gly Glu Gly Tyr Thr Asp 
                245                 250                 255     


Thr Ser Tyr Leu Asn Tyr Lys Phe Leu Ser Phe Phe Lys Glu Cys Trp 
            260                 265                 270         


Arg Pro Asn Ala Ile Ala Asn Asn Thr Gly Leu Leu Glu Gly Phe Trp 
        275                 280                 285             


Leu Ala Asn Asn Lys His Thr Asn Lys Lys Asn Gln Val Ala Tyr Ser 
    290                 295                 300                 


Phe Asn Pro Lys Ile Ser Glu Glu Leu Phe Arg Arg Arg Ser Leu Trp 
305                 310                 315                 320 


Glu Ser Asp Lys Cys Leu Leu Ser Asp Pro Arg Phe Glu Lys Tyr Val 
                325                 330                 335     


Glu Leu Phe Asp Lys His Gly Arg Tyr Arg Lys Gly Ala Ser Leu Thr 
            340                 345                 350         


Leu Ile Ser Lys Glu Ser Pro Ile Pro Ile Gly Phe Ser Met Asp Arg 
        355                 360                 365             


Asn Ala Ala Lys Leu Val Arg Ile Asp Asn Asp Thr Ala Asn Arg Gln 
    370                 375                 380                 


Leu Thr Ile Thr Ile Glu Leu Pro Asn Lys Glu Glu Arg Ser Tyr Val 
385                 390                 395                 400 


Ala Ala Tyr Gly Arg Lys His Glu Thr Lys Cys Tyr Tyr Asn Gly Leu 
                405                 410                 415     


Thr Thr Arg Leu Pro Arg Ser Glu Lys Glu Leu Leu Ala Leu Ala Lys 
            420                 425                 430         


Ala Glu Asn Arg Glu Leu Thr Asp Lys Glu Ile His Glu Ala Ser Leu 
        435                 440                 445             


Glu Lys Cys Tyr Ile Phe Glu Tyr Ala Arg Ala Gly Lys Ile Pro Val 
    450                 455                 460                 


Phe Ala Val Val Lys Thr Leu Tyr Phe Arg Arg Asn Pro Ser Asn Gly 
465                 470                 475                 480 


Glu Tyr Tyr Val Ile Leu Pro Thr Asn Ile Phe Val Glu Tyr His Ala 
                485                 490                 495     


Asn Asn Glu Phe Asn Ser Lys Glu Leu Phe Lys Ile Arg Ser Glu Leu 
            500                 505                 510         


Gln Lys Ala Trp Asp Glu Val Arg Thr Pro Lys Arg Asn Val Gln Ser 
        515                 520                 525             


Cys Val Leu Asp Lys Asp Leu Ser Lys Arg Phe Ala Gly Arg Thr Leu 
    530                 535                 540                 


Lys Tyr Ala Gly Ile Asp Leu Gly Tyr Ser Asn Pro Tyr Thr Val Ser 
545                 550                 555                 560 


Tyr Tyr Asn Val Val Gly Thr Glu Glu Gly Ile Gln Ile Lys Glu Thr 
                565                 570                 575     


Gly Asn Glu Ile Val Ser Thr Val Phe Asn Glu Gln Tyr Ile Gln Leu 
            580                 585                 590         


Lys Gly Asn Ile Tyr Gln Leu Ile Asn Ile Ile Arg Ala Ser Arg Arg 
        595                 600                 605             


Tyr Leu Gln Glu Ser Gly Glu Leu Lys Leu Ser Lys Asp Asp Ile Lys 
    610                 615                 620                 


Ser Phe Asp Gln Leu Met Glu Leu Leu Pro Ser Glu Gln Arg Ile Thr 
625                 630                 635                 640 


Ile Asp Gln Phe Ile Lys Asp Ile Lys Lys Ala Lys Gln Glu Gly Lys 
                645                 650                 655     


Leu Ile Arg Asp Ile Lys Gly Lys Leu Pro Val Glu Gly Lys Lys Lys 
            660                 665                 670         


Glu Tyr Trp Val Ile Ser Asn Leu Met Tyr Val Ile Thr Gln Thr Met 
        675                 680                 685             


Asn Gly Ile Arg Gly Asn Arg Asp Ser Asn Asn His Leu Thr Glu Lys 
    690                 695                 700                 


Lys Asn Trp Leu Ser Ala Pro Pro Leu Ile Glu Leu Ile Asp Ala Tyr 
705                 710                 715                 720 


Tyr Asn Leu Lys Lys Thr Phe Asn Asp Ser Gly Asp Gly Ile Lys Met 
                725                 730                 735     


Leu Pro Lys Asp His Val Tyr Ala Glu Gly Glu Lys Gln Arg Cys Thr 
            740                 745                 750         


Leu Arg Glu Glu Asn Phe Cys Lys Gly Ile Leu Glu Trp Arg Asp Asn 
        755                 760                 765             


Val Lys Asp Tyr Phe Ile Lys Lys Leu Phe Ser Gln Ile Ala His Arg 
    770                 775                 780                 


Cys Tyr Glu Leu Gly Ile Gly Ile Val Ala Met Glu Asn Leu Asp Ile 
785                 790                 795                 800 


Met Gly Ser Ser Lys Asn Thr Lys Gln Ser Asn Arg Met Phe Asn Ile 
                805                 810                 815     


Trp Pro Arg Gly Gln Met Lys Lys Ser Ala Glu Asp Ala Phe Ser Tyr 
            820                 825                 830         


Met Gly Ile Leu Ile Gln Tyr Val Asp Glu Asn Gly Thr Ser Arg His 
        835                 840                 845             


Asp Ala Asp Ser Gly Ile Tyr Gly Cys Arg Asp Gly Ala Asn Leu Trp 
    850                 855                 860                 


Leu Pro Asn Lys Lys Leu His Ala Asp Val Asn Ala Ser Arg Met Ile 
865                 870                 875                 880 


Ala Leu Arg Gly Leu Thr His His Thr Asn Leu Tyr Cys Arg Ser Leu 
                885                 890                 895     


Thr Glu Ile Glu Asn Gly Lys Tyr Val Asn Thr Tyr Glu Leu Phe Asp 
            900                 905                 910         


Thr Thr Lys Asn Asp Gln Ser Gly Ala Ala Lys Arg Leu Arg Gly Ala 
        915                 920                 925             


Glu Thr Leu Leu His Gly Tyr Ser Ala Thr Val Tyr Gln Ile His Thr 
    930                 935                 940                 


Thr Asn Thr Gly Ala Gly Val Ala Leu Leu Pro Asp Leu Thr Ala Thr 
945                 950                 955                 960 


Asp Val Ile Lys Asn Lys Lys Ile Thr Ala Thr Lys Glu Asn Thr Ala 
                965                 970                 975     


Lys Tyr Tyr Lys Leu Asp Asn Thr Asn Thr Tyr Tyr Pro Trp Ser Val 
            980                 985                 990         


Cys Glu Lys Leu His Lys Asn Trp  Lys Leu Ser Ser Arg  Ala Asp Pro 
        995                 1000                 1005             


Lys Lys  Lys Arg Lys Val 
    1010                 


<210>  83
<211>  885
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.4-NLSںϵ׵İ

<400>  83

Met Lys Lys Lys Lys Asn Phe Ser Val Ser Ala Thr Gly Val Phe Ser 
1               5                   10                  15      


Phe Pro Thr Thr Glu Ala Lys Met Asp Phe Phe His Arg Phe Ile Glu 
            20                  25                  30          


Leu Asn Gly Leu Ala Ala Glu Ile Glu Thr His Phe Leu Asn Leu Lys 
        35                  40                  45              


Asn Asp Lys Asn Gly Glu Ser Val Tyr Asn Lys Val Leu Ser Asn Ser 
    50                  55                  60                  


Asn His Ser Arg Pro Phe Ser Thr Pro Leu Leu Gly Thr Met Thr Gly 
65                  70                  75                  80  


Ser Thr Lys Val Thr Asp Lys Asn Ala Leu Tyr Gly Asn Asp Leu Asp 
                85                  90                  95      


His Cys Arg Lys Lys Lys Ile Val Pro Phe Ser Ser Ser Ser Pro Leu 
            100                 105                 110         


Ser Ser Gln Glu Lys Phe Phe Cys Ile Glu Ala Val Phe Arg Arg Ala 
        115                 120                 125             


Lys Ser His Met Glu Cys Lys Lys Leu Phe Gln Asp Glu Thr Asn Arg 
    130                 135                 140                 


Met Asp Ser Gln Ile Asn Gly Ile Leu Asn Glu Leu Pro Tyr Gly Val 
145                 150                 155                 160 


Glu Leu Ser Asn Met Leu Ser Glu Leu Ile Ala Ile Pro Phe Ala Ile 
                165                 170                 175     


Gly Trp Lys Leu Glu Gly Tyr Leu Gly Gln Val Phe Phe Pro Ser Ile 
            180                 185                 190         


Ala Glu Gly Leu Thr Pro Pro Lys Ser Ala Lys Ile Lys Gly Arg Arg 
        195                 200                 205             


Arg Ser Ile Asp Tyr Ser Val Thr Asp Glu Ala Tyr Asp Ile Leu Met 
    210                 215                 220                 


Lys Tyr Ser Asn Leu His Ser Ser Phe Glu Thr Gly Leu Lys Met Ser 
225                 230                 235                 240 


Asn Leu Phe Ser Ala Phe Tyr Lys Lys Ser Asn Arg Lys Asp Glu Ile 
                245                 250                 255     


Gln Phe Thr Pro Ile Ser Met Glu Ser Arg Cys Asp Leu Leu Leu Gly 
            260                 265                 270         


Lys Asn Phe Leu Lys Phe Asp Leu Lys Asn Cys Asp His Arg Ser Gly 
        275                 280                 285             


Ser Leu Met Leu Thr Ile Asn Asp Lys Asn Arg Leu Asn Gly Asp Tyr 
    290                 295                 300                 


Glu Ile Arg Val Gly Ser Asp Lys Lys Asp Ser Tyr Leu Thr Gly Val 
305                 310                 315                 320 


Asn Val Thr Asn Leu Gly Asp Asn Val Phe Asn Leu Asn Tyr Lys Val 
                325                 330                 335     


Asn Gly Lys Arg Glu Tyr Asn Met Leu Leu Lys Glu Pro Ser Ile His 
            340                 345                 350         


Ile Lys Met His Arg Met Arg Asp Asp Gly Asn Tyr Leu Ser Ser Asp 
        355                 360                 365             


Phe Asp Phe Tyr Met Ile Phe Ser Met Ser Ser Glu Lys Asp Glu Glu 
    370                 375                 380                 


Lys Leu Ala Arg Ser Trp Asp Met Arg Ala Ala Met Ser Thr Ala Tyr 
385                 390                 395                 400 


Gly Thr Asp Ile Lys Lys Tyr His Ser Ser Phe Pro Cys Arg Ile Leu 
                405                 410                 415     


Ala Cys Asp Leu Gly Val Lys His Pro Tyr Ser Ala Ala Val Met Asp 
            420                 425                 430         


Ile Gly Gln Leu Asn Glu Asn Gly Met Pro Val Ser Val Asp Lys Val 
        435                 440                 445             


His Cys Met His Ser Glu Gly Val Ser Glu Ile Gly Gln Gly Tyr Asn 
    450                 455                 460                 


His Leu Ile Gln Lys Ile Leu Ala Leu Asn Tyr Ile Leu Ala Tyr Cys 
465                 470                 475                 480 


Arg Glu Phe Val Ser Gly Thr Val Asp Asp Phe Asp Lys Ile Asp Tyr 
                485                 490                 495     


Lys Leu Ser Gln Leu Ser Tyr Lys Gln Glu Asp Leu Leu Ile Asn Leu 
            500                 505                 510         


Gln Glu Met Lys Asp His Phe Gly Asn Asp Met Gln Ala Trp Lys Lys 
        515                 520                 525             


Ser Arg Thr Trp Val Val Ser Thr Leu Phe Phe Glu Leu Arg Gln Glu 
    530                 535                 540                 


Phe Asn Gln Leu Arg Asn Gln Arg Pro Gly Lys Lys Thr Val Ser Leu 
545                 550                 555                 560 


Ala Asp Glu Phe Gln Tyr Ile Asp Met Arg Arg Lys Phe Ile Ser Leu 
                565                 570                 575     


Ser Arg Ser Tyr Thr Asn Val Gly Arg Gln Ser Ser Lys His Arg His 
            580                 585                 590         


Asp Ser Tyr Gln Thr His Tyr Asp Val Ile Asn Arg Cys Lys Lys Asn 
        595                 600                 605             


Leu Leu Arg Asn Ile Cys Arg Arg Met Ile Asp Met Ala Val Gln Asn 
    610                 615                 620                 


Lys Cys Asp Ile Ile Val Val Glu Asp Leu Ser Phe Gln Leu Ser Ser 
625                 630                 635                 640 


His Asn Ser Arg Arg Asp Asn Val Phe Asn Ala Leu Trp Ser Cys Lys 
                645                 650                 655     


Ser Ile Lys Asn Met Leu Gly Ile Met Ala Glu Gln His Asn Ile Ile 
            660                 665                 670         


Ile Ser Glu Val Asp Pro Asn His Thr Ser Lys Ile Asp Cys Glu Thr 
        675                 680                 685             


Gly Asn Phe Gly Tyr Arg Tyr Ser Ser Asp Phe Tyr Ser Val Ile Asp 
    690                 695                 700                 


Gly Gln Leu Val Arg Arg His Ala Asp Glu Asn Ala Ala Ile Asn Ile 
705                 710                 715                 720 


Gly Asn Arg Trp Ala Ser Arg His Thr Asp Leu Lys Ser Phe Asn Cys 
                725                 730                 735     


Arg Gln Ile Ser Ile Asp Gly Arg Lys Val Ala Phe Pro Tyr Ala Lys 
            740                 745                 750         


Gly Lys Arg Lys Ser Ala Leu Phe Gly Tyr Leu Phe Gly Asn Cys Lys 
        755                 760                 765             


Thr Val Phe Val Ser Asp Asp Gly Asp Ser Tyr Thr Pro Ile Pro Tyr 
    770                 775                 780                 


Ser Lys Phe Arg Lys Ser Ile Ser Lys Asp Asp His Asp Val Val Asn 
785                 790                 795                 800 


Tyr Leu His Asp Leu Thr Met Asn Lys Asn Val Ile Arg Val Glu Tyr 
                805                 810                 815     


Asn Lys Ser Ile Lys Ser Ala Ser Val Glu Leu Tyr Leu Asn Asp Asp 
            820                 825                 830         


Arg Val Ile Ser Arg Ser Leu Arg Asp Lys Glu Val Asp Ala Ile Glu 
        835                 840                 845             


Lys Leu Val Ser Arg Gly Ser Leu Ile Asn Glu Ser Gly Pro Ser Leu 
    850                 855                 860                 


Glu His Asp Glu Val Lys Ser Val Thr His Ser Arg Ala Asp Pro Lys 
865                 870                 875                 880 


Lys Lys Arg Lys Val 
                885 


<210>  84
<211>  881
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.5-NLSںϵ׵İ

<400>  84

Met Lys Val His Glu Ile Pro Arg Ser Gln Leu Leu Lys Ile Lys Gln 
1               5                   10                  15      


Tyr Glu Gly Ser Phe Val Glu Trp Tyr Arg Asp Leu Gln Glu Asp Arg 
            20                  25                  30          


Lys Lys Phe Ala Ser Leu Leu Phe Arg Trp Ala Ala Phe Gly Tyr Ala 
        35                  40                  45              


Ala Arg Glu Asp Asp Gly Ala Thr Tyr Ile Ser Pro Ser Gln Ala Leu 
    50                  55                  60                  


Leu Glu Arg Arg Leu Leu Leu Gly Asp Ala Glu Asp Val Ala Ile Lys 
65                  70                  75                  80  


Phe Leu Asp Val Leu Phe Lys Gly Gly Ala Pro Ser Ser Ser Cys Tyr 
                85                  90                  95      


Ser Leu Phe Tyr Glu Asp Phe Ala Leu Arg Asp Lys Ala Lys Tyr Ser 
            100                 105                 110         


Gly Ala Lys Arg Glu Phe Ile Glu Gly Leu Ala Thr Met Pro Leu Asp 
        115                 120                 125             


Lys Ile Ile Glu Arg Ile Arg Gln Asp Glu Gln Leu Ser Lys Ile Pro 
    130                 135                 140                 


Ala Glu Glu Trp Leu Ile Leu Gly Ala Glu Tyr Ser Pro Glu Glu Ile 
145                 150                 155                 160 


Trp Glu Gln Val Ala Pro Arg Ile Val Asn Val Asp Arg Ser Leu Gly 
                165                 170                 175     


Lys Gln Leu Arg Glu Arg Leu Gly Ile Lys Cys Arg Arg Pro His Asp 
            180                 185                 190         


Ala Gly Tyr Cys Lys Ile Leu Met Glu Val Val Ala Arg Gln Leu Arg 
        195                 200                 205             


Ser His Asn Glu Thr Tyr His Glu Tyr Leu Asn Gln Thr His Glu Met 
    210                 215                 220                 


Lys Thr Lys Val Ala Asn Asn Leu Thr Asn Glu Phe Asp Leu Val Cys 
225                 230                 235                 240 


Glu Phe Ala Glu Val Leu Glu Glu Lys Asn Tyr Gly Leu Gly Trp Tyr 
                245                 250                 255     


Val Leu Trp Gln Gly Val Lys Gln Ala Leu Lys Glu Gln Lys Lys Pro 
            260                 265                 270         


Thr Lys Ile Gln Ile Ala Val Asp Gln Leu Arg Gln Pro Lys Phe Ala 
        275                 280                 285             


Gly Leu Leu Thr Ala Lys Trp Arg Ala Leu Lys Gly Ala Tyr Asp Thr 
    290                 295                 300                 


Trp Lys Leu Lys Lys Arg Leu Glu Lys Arg Lys Ala Phe Pro Tyr Met 
305                 310                 315                 320 


Pro Asn Trp Asp Asn Asp Tyr Gln Ile Pro Val Gly Leu Thr Gly Leu 
                325                 330                 335     


Gly Val Phe Thr Leu Glu Val Lys Arg Thr Glu Val Val Val Asp Leu 
            340                 345                 350         


Lys Glu His Gly Lys Leu Phe Cys Ser His Ser His Tyr Phe Gly Asp 
        355                 360                 365             


Leu Thr Ala Glu Lys His Pro Ser Arg Tyr His Leu Lys Phe Arg His 
    370                 375                 380                 


Lys Leu Lys Leu Arg Lys Arg Asp Ser Arg Val Glu Pro Thr Ile Gly 
385                 390                 395                 400 


Pro Trp Ile Glu Ala Ala Leu Arg Glu Ile Thr Ile Gln Lys Lys Pro 
                405                 410                 415     


Asn Gly Val Phe Tyr Leu Gly Leu Pro Tyr Ala Leu Ser His Gly Ile 
            420                 425                 430         


Asp Asn Phe Gln Ile Ala Lys Arg Phe Phe Ser Ala Ala Lys Pro Asp 
        435                 440                 445             


Lys Glu Val Ile Asn Gly Leu Pro Ser Glu Met Val Val Gly Ala Ala 
    450                 455                 460                 


Asp Leu Asn Leu Ser Asn Ile Val Ala Pro Val Lys Ala Arg Ile Gly 
465                 470                 475                 480 


Lys Gly Leu Glu Gly Pro Leu His Ala Leu Asp Tyr Gly Tyr Gly Glu 
                485                 490                 495     


Leu Ile Asp Gly Pro Lys Ile Leu Thr Pro Asp Gly Pro Arg Cys Gly 
            500                 505                 510         


Glu Leu Ile Ser Leu Lys Arg Asp Ile Val Glu Ile Lys Ser Ala Ile 
        515                 520                 525             


Lys Glu Phe Lys Ala Cys Gln Arg Glu Gly Leu Thr Met Ser Glu Glu 
    530                 535                 540                 


Thr Thr Thr Trp Leu Ser Glu Val Glu Ser Pro Ser Asp Ser Pro Arg 
545                 550                 555                 560 


Cys Met Ile Gln Ser Arg Ile Ala Asp Thr Ser Arg Arg Leu Asn Ser 
                565                 570                 575     


Phe Lys Tyr Gln Met Asn Lys Glu Gly Tyr Gln Asp Leu Ala Glu Ala 
            580                 585                 590         


Leu Arg Leu Leu Asp Ala Met Asp Ser Tyr Asn Ser Leu Leu Glu Ser 
        595                 600                 605             


Tyr Gln Arg Met His Leu Ser Pro Gly Glu Gln Ser Pro Lys Glu Ala 
    610                 615                 620                 


Lys Phe Asp Thr Lys Arg Ala Ser Phe Arg Asp Leu Leu Arg Arg Arg 
625                 630                 635                 640 


Val Ala His Thr Ile Val Glu Tyr Phe Asp Asp Cys Asp Ile Val Phe 
                645                 650                 655     


Phe Glu Asp Leu Asp Gly Pro Ser Asp Ser Asp Ser Arg Asn Asn Ala 
            660                 665                 670         


Leu Val Lys Leu Leu Ser Pro Arg Thr Leu Leu Leu Tyr Ile Arg Gln 
        675                 680                 685             


Ala Leu Glu Lys Arg Gly Ile Gly Met Val Glu Val Ala Lys Asp Gly 
    690                 695                 700                 


Thr Ser Gln Asn Asn Pro Ile Ser Gly His Val Gly Trp Arg Asn Lys 
705                 710                 715                 720 


Gln Asn Lys Ser Glu Ile Tyr Phe Tyr Glu Asp Lys Glu Leu Leu Val 
                725                 730                 735     


Met Asp Ala Asp Glu Val Gly Ala Met Asn Ile Leu Cys Arg Gly Leu 
            740                 745                 750         


Asn His Ser Val Cys Pro Tyr Ser Phe Val Thr Lys Ala Pro Glu Lys 
        755                 760                 765             


Lys Asn Asp Glu Lys Lys Glu Gly Asp Tyr Gly Lys Arg Val Lys Arg 
    770                 775                 780                 


Phe Leu Lys Asp Arg Tyr Gly Ser Ser Asn Val Arg Phe Leu Val Ala 
785                 790                 795                 800 


Ser Met Gly Phe Val Thr Val Thr Thr Lys Arg Pro Lys Asp Ala Leu 
                805                 810                 815     


Val Gly Lys Arg Leu Tyr Tyr His Gly Gly Glu Leu Val Thr His Asp 
            820                 825                 830         


Leu His Asn Arg Met Lys Asp Glu Ile Lys Tyr Leu Val Glu Lys Glu 
        835                 840                 845             


Val Leu Ala Arg Arg Val Ser Leu Ser Asp Ser Thr Ile Lys Ser Tyr 
    850                 855                 860                 


Lys Ser Phe Ala His Val Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys 
865                 870                 875                 880 


Val 
    


<210>  85
<211>  975
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.6-NLSںϵ׵İ

<400>  85

Met Ser Ala Asn Arg Val Ser Ala Asn Ser Gln Phe Glu Leu Gly Tyr 
1               5                   10                  15      


Pro Met Ser Leu Ser Leu Arg Gly Lys Val Phe Asn Ser Arg Glu Met 
            20                  25                  30          


Met Lys Glu Ile Leu Pro Val Met Asn Asn Ile Val His Tyr Gln Asn 
        35                  40                  45              


Asn Leu Leu Lys Leu Met Leu Ile Leu Arg Gly Glu Lys Tyr Thr Leu 
    50                  55                  60                  


Asp Gly Gln Phe Phe Ser Gln Lys Asp Val Asp Arg Gln Phe Gly Asp 
65                  70                  75                  80  


Leu Cys Lys Glu His Asn Ile Lys Gly Ser Ile Cys Ser Leu Lys Glu 
                85                  90                  95      


Lys Ser Arg Lys Leu Tyr Glu Val Phe Ser Cys Tyr Ile Asp Lys Lys 
            100                 105                 110         


Gly Asn Leu Lys Thr Asn Ser Lys Ala Arg Ser Phe Ala Gly Val Leu 
        115                 120                 125             


Leu Asn Pro Lys Asp Val Lys Leu Pro Pro Gln Ile Asp Ser Ile Ser 
    130                 135                 140                 


Ser Phe Val Val Glu Leu Arg Ala Lys Gly Val Leu Pro Ile Lys His 
145                 150                 155                 160 


Glu Gly Asn Tyr Leu Ser Gly His Pro Ser Leu Lys Tyr Ser Val Ala 
                165                 170                 175     


Gln Asn Val Leu Val Lys Leu Thr Ser Met Glu Lys Leu Gln Lys Ile 
            180                 185                 190         


Tyr Ser Asp Glu Lys Ala Gly Trp Glu Asn Ile Val Ser Glu Val Arg 
        195                 200                 205             


Ser Asp Leu Pro Lys Ile Glu Arg Tyr Glu Arg Met Leu Leu Ser Ile 
    210                 215                 220                 


Lys Ala Val Lys Glu Met Glu Lys Phe Gly Ile Asn Asn Tyr Arg His 
225                 230                 235                 240 


Leu Leu Asn Asn Trp Arg Asp Glu Val Asp Lys Asp Ser Gly Lys Val 
                245                 250                 255     


Leu Lys Gln Gly Met Arg Thr Tyr Phe Val Asn Met Leu Glu Ser Lys 
            260                 265                 270         


Lys Asp Tyr Arg Phe Glu Glu Ser Asp Arg Tyr Leu Phe Gly Tyr Ala 
        275                 280                 285             


Pro Glu Val Met Asn Leu Val Tyr His Asp Phe Arg Asp Leu Trp Gln 
    290                 295                 300                 


Gly Glu Asp Ile Ile Gly Ser Gln Ser Pro Glu Lys Lys Asp Arg Asp 
305                 310                 315                 320 


Tyr Val Asp Val Ile Phe Asn Tyr Phe Asn Trp Arg Lys Glu Ser Ile 
                325                 330                 335     


Asn Ile Ser Ser Phe Asp Ser Tyr Gly Lys Thr Ala Gln Ile Lys Leu 
            340                 345                 350         


Gly Asp Asn Tyr Val Pro Phe Ser Asn Phe Gln Tyr Asp Lys Ile Leu 
        355                 360                 365             


Asp Ala Trp Thr Leu Glu Ile Ala Asn Val Ser Gly Glu Gly Asp Asn 
    370                 375                 380                 


His Lys Leu Val Ile Ala Arg Ser Pro Gln Phe Asp Ser His Ser Ser 
385                 390                 395                 400 


Val Lys Asp Ile Val Met Lys Asn Leu Lys Gly Lys Glu Ala Ser Lys 
                405                 410                 415     


Thr Thr Leu Glu Phe Arg Tyr Ser Gly Asp Ser Lys Lys Ser Thr Trp 
            420                 425                 430         


Tyr Arg Gly Thr Leu Lys Glu Pro Thr Leu Arg Tyr Ser Ser Ser Lys 
        435                 440                 445             


Asn Cys Leu Tyr Val Asp Phe Ala Leu Ser Asn His Ile Val Glu Gly 
    450                 455                 460                 


Leu Ile Ser Asp Asn Leu Gly Ile Ser Asp Lys Met Tyr Lys Phe Arg 
465                 470                 475                 480 


Gly Glu Phe Met Lys Ala Ser Pro Ser Ser Gly Lys Gln Ser Asn Ser 
                485                 490                 495     


Ile Asn Leu Pro Ile Lys Lys Leu Arg Ala Met Gly Val Asp Phe Asn 
            500                 505                 510         


Leu Arg Arg Pro Phe Gln Ala Ser Ile Tyr Asp Val Glu Asn Lys Asn 
        515                 520                 525             


Gly Asn Leu Glu Phe Ser Phe Val Lys His Val Gln Ser Phe Ser Asn 
    530                 535                 540                 


Glu Asn Asp Glu Glu Arg Ala Lys Glu Leu Leu Asn Ile Glu Arg Asn 
545                 550                 555                 560 


Ile Leu Ala Leu Lys Ile Leu Ile Trp Gln Thr Val Gly Tyr Val Thr 
                565                 570                 575     


Gly Lys Asn Asp Thr Ile Asp Gly Val Val Thr Arg Lys Asn Asn Ala 
            580                 585                 590         


Val Asp Ile Glu Lys Thr Leu Gly Ile Asn Met Lys Glu Tyr Met Ala 
        595                 600                 605             


Tyr Leu Asn Gln Phe Arg Ser Tyr Glu Asp Lys Asn Lys Ala Phe Met 
    610                 615                 620                 


Asp Leu Arg Lys Arg Glu Tyr Ala Trp Ile Val Pro Pro Leu Ile Phe 
625                 630                 635                 640 


Gln Cys Arg Ser Arg Leu Ile Ser Phe Arg Ser Glu Tyr Phe Asn Thr 
                645                 650                 655     


Pro Lys Asp Glu Lys Ser His Tyr Cys Gln His Arg Asn Phe Val Asp 
            660                 665                 670         


Tyr Ser Thr Phe Leu Lys Lys Asn Val Val Lys Lys Met Met Glu Leu 
        675                 680                 685             


Arg Arg Ser Tyr Ser Thr Phe Gly Met Ser Ser Glu Gln Ser Ile Trp 
    690                 695                 700                 


Val Thr Asn Asn Asp His Ala Lys Asp Gly Ser Lys Lys Asn Gly Asn 
705                 710                 715                 720 


Met Phe Asp Asp Asp Leu His Gln Trp Tyr Asn Gly Leu Val Arg Lys 
                725                 730                 735     


Cys Ser Ser Leu Ala Ser Ser Ile Ile Asn Val Ala Arg Asp Asn Gly 
            740                 745                 750         


Ala Ile Leu Val Phe Ile Glu Asp Leu Asp Cys His Pro Ser Ala Phe 
        755                 760                 765             


Asp Ser Glu Glu Asp Asn Ser Leu Lys Ser Ile Trp Gly Trp Gly Ser 
    770                 775                 780                 


Ile Lys Ala Ser Leu Ala His Gln Ala Arg Lys His Asn Ile Ala Val 
785                 790                 795                 800 


Val Ala Asn Asp Pro His Leu Thr Ser Leu Val Ser Ser Thr Thr Gly 
                805                 810                 815     


Glu Leu Gly Ile Ala Lys Gly Arg Asp Val Leu Phe Phe Asp Ser Lys 
            820                 825                 830         


Gly Lys Leu Thr Ser Lys Val Asn Arg Asp Glu Asn Ala Ala Gln Asn 
        835                 840                 845             


Ile Ala Ile Arg Gly Phe Val Arg His Ser Asp Leu Arg Glu Phe Val 
    850                 855                 860                 


Ala Glu Lys Ile Glu Glu Asn Arg Tyr Arg Val Val Val Asn Lys Thr 
865                 870                 875                 880 


His Lys Arg Lys Ala Gly Ala Ile Tyr Arg His Ile Gly Ser Thr Glu 
                885                 890                 895     


Cys Ile Met Ser Lys Gln Ala Asp Gly Ser Leu Lys Ile Asp Lys Thr 
            900                 905                 910         


Glu Leu Thr Pro Leu Glu Ile Lys Met Glu Lys Lys Asn Asp Lys Lys 
        915                 920                 925             


Met Tyr Val Ile Leu His Gly Lys Thr Trp Arg Leu Arg His Glu Leu 
    930                 935                 940                 


Asn Glu Lys Leu Glu Lys Asp Leu Asp Asn His Leu Lys Ser Lys Ser 
945                 950                 955                 960 


Ser Val Ile Ser Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
                965                 970                 975 


<210>  86
<211>  973
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.7-NLSںϵ׵İ

<400>  86

Met Ser Ser Ala Asn Asp Gln Leu Gly Leu Gly Tyr Pro Leu Thr Leu 
1               5                   10                  15      


Thr Leu Arg Gly Lys Val Tyr Asn His Asp Thr Ala Met Glu Ala Phe 
            20                  25                  30          


Ala Pro Val Met Lys Gly Met Val Pro Tyr Ala Asn Asn Leu Met Arg 
        35                  40                  45              


Ile Leu Leu Thr Leu Arg Leu Glu Lys Tyr Thr Leu Asp Gly Ile His 
    50                  55                  60                  


His Thr Lys Glu Glu Val Glu Lys Asp Leu Arg Gly Leu Met Lys Glu 
65                  70                  75                  80  


Tyr Gly Ile Asn Leu Ser Phe Ala Lys Phe Ser Glu Met Ala Gly Glu 
                85                  90                  95      


Val Tyr Arg Val Phe Val Cys Tyr Val Asp Ala Lys Gly Lys Leu Lys 
            100                 105                 110         


Val Asn Gly Lys Ala Arg Gly Phe Ala Asn Val Phe Phe Ser Glu Asp 
        115                 120                 125             


Asp Ala Thr Ile Pro Glu Asn Cys Pro Ser Met Glu Leu Leu Arg Lys 
    130                 135                 140                 


Lys Gly Met Phe Pro Ile Leu Val Asp Gly Lys Pro Ile Ser Ser Ile 
145                 150                 155                 160 


Ser Arg Glu Lys Thr Pro Leu Lys Tyr Ser Val Ala Gln Asp Val Leu 
                165                 170                 175     


Thr Lys Leu Thr Ser Met Glu Glu Ile Ser Lys Glu Tyr Glu Lys Ala 
            180                 185                 190         


Lys Thr Asp Trp Glu Asn Glu Cys Gln Lys Val Ile Ser Gln Leu Pro 
        195                 200                 205             


Leu Ile Gly Arg Tyr Glu Ala Leu Leu Thr Thr Ile Pro Leu Ile Pro 
    210                 215                 220                 


Glu Met Arg Gly Phe Asp Gly Asp Asn Tyr Arg Lys Met Leu Asn Arg 
225                 230                 235                 240 


Trp Arg Asp Tyr Val Asn Glu Asp Gly Glu Leu Val Arg Gly Gly Met 
                245                 250                 255     


Lys Thr Tyr Phe Leu Asp Leu Leu Ser Lys Asp Thr Ser His Lys Phe 
            260                 265                 270         


Asn Glu Glu Glu Arg Tyr Leu Phe Gly Tyr Cys Pro Glu Phe Met Asn 
        275                 280                 285             


Leu Ile Tyr His Asp Phe Arg Asp Leu Trp Ser Lys Glu Asp Ile Ile 
    290                 295                 300                 


Gly Ser Gln Arg Lys Gly Lys Gly Leu Lys Gly Lys Asp Tyr Val Asp 
305                 310                 315                 320 


Val Ile Phe Asn Cys Phe His Trp Arg Arg Glu Ser Ile Asn Ile Ser 
                325                 330                 335     


Ser Phe Gly Asn Asn Asp Lys Val Met Asn Ile His Leu Gly Asp Asn 
            340                 345                 350         


Phe Val Pro Phe Glu Leu Lys Ser Gln Asn Gly Ile Trp Glu Val His 
        355                 360                 365             


Val Gln Asn Leu His Gly Gln Asn Asp Pro His Arg Val Ile Val Cys 
    370                 375                 380                 


Arg Cys Pro Gln Phe Asn Glu Asp Ser Ser Met Lys Met Val His Pro 
385                 390                 395                 400 


Leu Ala Lys Asn Gly Glu Glu Ser Asp Lys Glu Asn Ile Glu Phe Arg 
                405                 410                 415     


Tyr Ser Gly Asp Ser Lys Arg Glu Thr Trp Tyr Thr Gly Leu Leu Lys 
            420                 425                 430         


Glu Pro Thr Leu Arg Tyr Asp Val Glu Arg Lys Ser Leu Tyr Val Asp 
        435                 440                 445             


Phe Ile Leu Ser Asn His Arg Val Glu Gly Val Val Thr Asn Glu Tyr 
    450                 455                 460                 


Leu Lys Asp Pro Arg Asp Leu Phe Gly Val Arg Gly Tyr Phe Leu Ser 
465                 470                 475                 480 


Ser Ser Val Ser Asn Pro Arg Gln Lys Asp Lys Thr Ser Leu Pro Asp 
                485                 490                 495     


Gly Lys Phe Asn Val Met Gly Val Asp Leu Gly Leu Lys Cys Pro Tyr 
            500                 505                 510         


Glu Cys Ala Ile Tyr Gly Ile Thr Val Lys Asn Gly Lys Met Gln His 
        515                 520                 525             


Lys Trp Ser His Asn Val Ser Ala Glu Asp Asn Asn Asn Val Ser Glu 
    530                 535                 540                 


Arg Leu Ala Asn Leu Lys Lys Ile Asp Glu Lys Ile Leu Ala Thr Gln 
545                 550                 555                 560 


Val Leu Ile Ser Leu Thr Lys Met Cys Val Val Lys Asp Glu Glu Ile 
                565                 570                 575     


Pro Asp Ser Tyr Thr Leu Arg Glu His Arg Val Asp Ile Ala Lys Ser 
            580                 585                 590         


Leu Asp Leu Asp Met Asp Lys Tyr Arg Arg Tyr Val Glu Lys Cys Lys 
        595                 600                 605             


Lys Asn Pro Asp Lys Ile Gln Ala Leu Lys Asp Ile Arg Lys Ser Glu 
    610                 615                 620                 


Asn Asn Trp Ile Val Ala Glu Lys Ile Asn Glu Ile Arg Ser Leu Ile 
625                 630                 635                 640 


Ser Glu Ile Arg Ser Glu Tyr Tyr Ala Ser Lys Asp Lys Arg Asn Tyr 
                645                 650                 655     


Cys Arg Asn Leu Asn Gly Val Asp Leu Ser Val Phe Leu Lys Lys Lys 
            660                 665                 670         


Val Val Lys Asn Trp Ile Ser Leu Leu Arg Ser Phe Ser Thr Phe Gly 
        675                 680                 685             


Met Thr Pro Gln Glu Ser Ala Tyr Ile Arg Lys Asp Phe Ala Lys Asn 
    690                 695                 700                 


Leu Ser Lys Trp Tyr Lys Gly Leu Val Arg Lys Cys Gly Ser Ile Ala 
705                 710                 715                 720 


Ala His Ile Val Asn Ile Ala Arg Asp Asn Lys Val Met Val Ile Phe 
                725                 730                 735     


Ile Glu Asp Leu Asp Ala Arg Thr Ser Ala Phe Asp Ser Lys Glu Asp 
            740                 745                 750         


Asn Glu Leu Lys Ile Leu Trp Gly Trp Gly Glu Ile Lys Lys Trp Ile 
        755                 760                 765             


Gly His Gln Ala Arg Lys His Asn Ile Ala Val Val Ala Val Asp Pro 
    770                 775                 780                 


His Leu Thr Ser Leu Val Asn His Glu Ser Gly Leu Leu Gly Ile Ala 
785                 790                 795                 800 


Gly Ser Gly Asn Asp Arg Asn Ile Tyr Thr Phe Gln Lys Asn Lys Lys 
                805                 810                 815     


Tyr Val Val Ile Asn Arg Asp Asn Asn Ala Ala His Asn Ile Ala Leu 
            820                 825                 830         


Arg Gly Leu Ser Lys His Thr Asp Ile Arg Glu Phe Tyr Val Glu Gln 
        835                 840                 845             


Ile Asp Val Asp His Tyr Arg Leu Met Tyr Gly Pro Glu Ala Glu Asn 
    850                 855                 860                 


Gly Lys Arg Arg Ser Gly Ala Ile Tyr Lys His Ile Gly Ser Thr Glu 
865                 870                 875                 880 


Cys Val Phe Ser Lys Gln Lys Asn Gly Thr Leu Lys Val Glu Lys Thr 
                885                 890                 895     


Ser Leu Thr Lys Asp Glu Lys Glu Met Pro Lys Ile Asn Gly Lys Gly 
            900                 905                 910         


Val Tyr Ala Ile Leu His Gly Asn Glu Trp Arg Leu Arg His Glu Leu 
        915                 920                 925             


Asn Glu Glu Leu Gly Ala Lys Leu Asp Gly Ile Ser Val Lys Arg Val 
    930                 935                 940                 


Val Ser Glu Pro Asn Lys Val Lys Thr Ser Leu Val Lys Gly Ser Val 
945                 950                 955                 960 


Arg Ala Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
                965                 970             


<210>  87
<211>  918
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.8-NLSںϵ׵İ

<400>  87

Met Lys Lys Gln Thr Ile Val Lys Lys Asp Ser Lys Ala Glu Thr Lys 
1               5                   10                  15      


Glu Asn Lys Met Tyr Pro Asp Lys Asp Thr Asp Phe Pro Val Asn Ser 
            20                  25                  30          


Gln Phe Ser Arg Ser Ile Ser Ile Arg Ala Asn Val Asp Pro Lys Asp 
        35                  40                  45              


Leu Leu Val Leu Lys Arg Thr Phe Glu Glu Thr Thr Lys Ile Ser Asp 
    50                  55                  60                  


Glu Leu Leu Ser Thr Leu Leu Met Leu Arg Gly Lys Asp Tyr Cys Leu 
65                  70                  75                  80  


Asp Asn Val Val Cys Lys Gly Glu Glu Val Leu Glu Asn Leu Tyr Lys 
                85                  90                  95      


Lys Leu Ser Lys Asn Ala Thr Val Asn Arg Asp Lys Phe Ile Ser Thr 
            100                 105                 110         


Ala Lys Ala Phe Tyr Glu Tyr Phe His Gly Cys Ser Tyr His Lys Gly 
        115                 120                 125             


Phe Lys Ser Phe Phe Phe Ser Ser Lys Glu Ile Asp Ser Ile Gln Ser 
    130                 135                 140                 


Glu Lys Phe Gly Tyr Leu Arg Glu Ile Gly Leu Phe Pro Ile Lys Ile 
145                 150                 155                 160 


Asp Ala Gln Ile Ser Asn Asp Leu Gln Tyr Ser Ile Val Ala Ser Asn 
                165                 170                 175     


His Ala Lys Ile Lys Gly Phe Glu Lys Ile Asp Lys Glu Tyr Gln Ala 
            180                 185                 190         


Asn Lys Glu Lys Trp Asn Lys Thr Ile Gly Glu Ser Thr Leu Lys His 
        195                 200                 205             


Leu Asn Arg Tyr Gly Glu Met Leu Lys Gly Leu Ser Asp Leu Gly Thr 
    210                 215                 220                 


Met Gly Asn Phe Asn Gly Lys Lys Tyr Asp Arg Phe Met Gly His Trp 
225                 230                 235                 240 


Arg Asn Glu Gln Lys Ile Pro Asp His Ile Ser Met Leu Asp Phe Phe 
                245                 250                 255     


Arg Lys Ile Tyr Gln Glu Lys Gly Lys Ser His Arg Phe Thr Ala Ile 
            260                 265                 270         


Asp Asn Phe Thr Tyr Gly Tyr Glu Ser Glu Phe Met Asn His Ile Tyr 
        275                 280                 285             


Leu Asn Phe Ser Asp Leu Trp Leu Lys Glu Asp Val Ile Gly Asp Glu 
    290                 295                 300                 


Glu Tyr Val Ser Leu Ile Arg Gly Ala Tyr His Trp Gln Lys Asp Val 
305                 310                 315                 320 


Val Gly Ile Ala Ser Phe Ser Gly Tyr Asn Lys Tyr Glu Lys Leu Phe 
                325                 330                 335     


Met Gly Asp Asn Lys Ile Asn Tyr Ala Leu Asp Phe Ser Asn Lys Asp 
            340                 345                 350         


Gln Trp Leu Met Lys Phe Asn Asn Val Ile Ser Lys Glu Pro Glu Thr 
        355                 360                 365             


Ile Thr Leu Arg Leu Cys Lys Asn Gly Tyr Phe Asn Asn Leu Ser Val 
    370                 375                 380                 


Leu Glu Lys Asn Asp Glu Asn Gly Arg Tyr Lys Ile Arg Phe Ser Thr 
385                 390                 395                 400 


Glu Lys Gln Gly Lys Tyr Phe Tyr Glu Ala Phe Ile Arg Glu Pro Phe 
                405                 410                 415     


Leu Arg Tyr Asn Lys Asp Asn Asp Lys Ile Tyr Val His Phe Cys Leu 
            420                 425                 430         


Ser Glu Glu Ile Lys Glu Asn Cys Pro Asn His Leu Asp Thr Arg Ser 
        435                 440                 445             


Asp Lys Tyr Leu Phe Lys Ser Ala Leu Leu Thr Asn Ser Arg Gln Lys 
    450                 455                 460                 


Leu Gly Lys Leu His Tyr Arg Asp Phe His Ile Val Gly Val Asp Leu 
465                 470                 475                 480 


Gly Ile Asn Pro Val Ala Lys Ile Thr Val Cys Lys Val His Val Asp 
                485                 490                 495     


Lys Asn Glu Asn Leu Lys Ile Thr Lys Ile Ile Thr Glu Glu Thr Arg 
            500                 505                 510         


Lys Asn Ile Asp Thr Asn Tyr Leu Asp Gln Leu Asn Leu Leu Tyr Lys 
        515                 520                 525             


Lys Ile Val Ser Leu Lys Arg Leu Ile Arg Ala Thr Val Ala Phe Lys 
    530                 535                 540                 


Lys Asp Gly Glu Glu Ile Pro Lys Met Phe Lys Met Gly Lys Lys Ser 
545                 550                 555                 560 


Pro Tyr Phe Leu Asn Trp Thr Glu Val Leu Asn Val Asn Tyr Asp Asp 
                565                 570                 575     


Tyr Ile Lys Glu Ile Ser Thr Phe Ser Val Asp Arg Leu Ser Gly Leu 
            580                 585                 590         


Thr Leu Pro Met Gln Trp Ala Arg Ser Gln Asn Lys Trp Val Val Lys 
        595                 600                 605             


Asp Leu Thr Lys Met Val Arg Lys Gly Ile Ser Asp Leu Ile Tyr Ala 
    610                 615                 620                 


Arg Tyr Phe Asn Cys Ser Asp Lys Thr Gln Tyr Val Thr Glu Asn Asn 
625                 630                 635                 640 


Ala Val Asp Ile Thr Thr Phe Lys Lys His Asp Ile Ile Ser Glu Ile 
                645                 650                 655     


Ile Gly Leu Gln Lys Met Phe Ser Gly Gly Gly Lys Asp Val Ala Lys 
            660                 665                 670         


Lys Asp Tyr Leu Tyr Leu Arg Gly Leu Arg Lys His Ile Gly Asn Tyr 
        675                 680                 685             


Thr Ala Ser Ala Ile Val Ser Ile Ala Gln Lys Tyr Asn Ala Val Phe 
    690                 695                 700                 


Ile Phe Ile Glu Asp Leu Asp Leu Lys Ile Ser Gly Met Asn Gly Lys 
705                 710                 715                 720 


Lys Glu Asn Lys Val Lys Ile Leu Trp Gly Val Gly Gln Leu Lys Lys 
                725                 730                 735     


Arg Leu Ser Glu Lys Ala Glu Lys Phe Gly Ile Gly Ile Val Pro Val 
            740                 745                 750         


Asn Pro Glu Leu Thr Ser Gln Met Asp Arg Glu Thr Phe Leu Leu Gly 
        755                 760                 765             


Tyr Arg Asn Pro Thr Asn Lys Lys Glu Leu Tyr Val Lys Arg Asp Asp 
    770                 775                 780                 


Lys Ile Glu Ile Leu Asp Ala Asp Glu Thr Ala Ser Tyr Asn Val Ala 
785                 790                 795                 800 


Leu Arg Gly Leu Gly His His Ala Asn Leu Ile Gln Phe Arg Ala Asp 
                805                 810                 815     


Lys Met Pro Asn Gly Cys Phe Arg Val Met Pro Asp Arg Lys Tyr Lys 
            820                 825                 830         


Gln Gly Ala Leu Tyr Gly Tyr Leu Asn Ser Thr Ala Val Leu Phe Lys 
        835                 840                 845             


Asp Lys Gly Asp Gly Val Leu Thr Ile His Lys Ser Lys Leu Thr Lys 
    850                 855                 860                 


Lys Glu Arg Asp Ser Arg Pro Ile Lys Gly Lys Lys Thr Phe Val Val 
865                 870                 875                 880 


Lys Asn Gly Lys Arg Trp Ile Leu Arg His Val Leu Asp Glu Glu Val 
                885                 890                 895     


Lys Lys Tyr Pro Glu Met Tyr Asn Ser Gln Asn Ser Arg Ala Asp Pro 
            900                 905                 910         


Lys Lys Lys Arg Lys Val 
        915             


<210>  88
<211>  923
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.9-NLSںϵ׵İ

<400>  88

Met Ser Asp Tyr Lys Phe Ser Asn Asn Gly Val Thr Asn Thr Gly Ser 
1               5                   10                  15      


Ala His Ile Gly Leu Ser Pro Glu Asn Ser Ser Thr Val Met Asp Met 
            20                  25                  30          


Phe Lys Val Ile Thr Lys Asp Ala Asp Phe Leu Leu Lys Asn Leu Leu 
        35                  40                  45              


Ile Met Glu Gly Gly Glu Tyr Met Leu Asn Arg Glu Ile His Asn Gly 
    50                  55                  60                  


Asp Lys Glu Phe Asp Lys Ile Ile Ser Lys Leu Gly Leu Ser Lys Lys 
65                  70                  75                  80  


Glu Lys Glu Asn Leu Lys Met Lys Cys Lys Asp Phe Phe Phe Asp Phe 
                85                  90                  95      


Val Lys Leu Gln Asn Gly Arg Ser Leu Ala Asn Ile Leu Phe Glu Thr 
            100                 105                 110         


Lys Gly Thr Thr Leu Ile Gly Cys Gly Lys Asp Lys Lys Gly Glu Lys 
        115                 120                 125             


Val Asp Gly Glu Tyr Pro Thr Ile Tyr His Asp His Glu Thr Leu Arg 
    130                 135                 140                 


Ser Thr Gly Leu Leu Pro Leu Lys Phe Ser Lys Asn Ile Asp Asp Val 
145                 150                 155                 160 


Asp Tyr Lys Tyr Leu Ile Cys Tyr Leu Val His Asn Val Leu Ser Ser 
                165                 170                 175     


Phe Ile Glu Lys Arg Asp Ala Tyr Asn Asp Asn Lys Lys Glu Trp Glu 
            180                 185                 190         


Ser Lys Leu Ser Asn Ser Asn Leu Pro Gln Leu Glu Arg Met Ser Glu 
        195                 200                 205             


Phe Leu Asn Gly Ile Asn His Leu Gly Asn Ile Ile Gly Trp Asn Gly 
    210                 215                 220                 


Lys Lys Tyr Ile Gly Phe Ile Lys Lys Trp Thr Asp Glu Glu Ser Ser 
225                 230                 235                 240 


Met Tyr Asp Phe Phe Val Gln Lys Leu Gln Asp Asn Pro Lys Tyr Lys 
                245                 250                 255     


Phe Gly Lys Lys Asp Gln Phe Leu Tyr Gly Tyr Glu Pro Glu Phe Leu 
            260                 265                 270         


Asn Tyr Leu Phe His Asp Phe Arg Asp Leu Trp His Pro Asp Asn Leu 
        275                 280                 285             


Ile Gly Lys Asp Glu Tyr Val Asp Leu Ile Ser Gly Lys Asn Asn Thr 
    290                 295                 300                 


Asp Ala Glu Thr Ala Asn Lys Gly Ala Tyr His Trp Leu Lys Asp Phe 
305                 310                 315                 320 


Ile Asn Ile Ser Ser Phe Asp Ala Tyr Gly Lys Met Ala Thr Ile Gly 
                325                 330                 335     


Met Gly Asn Asn Leu Ile Asn Tyr Ser Met Asn Ile Asp Lys Asp Gly 
            340                 345                 350         


Lys Ile Ile Val Asn Met Asp Asn Ile Phe Asp Arg Ser Lys Pro Ile 
        355                 360                 365             


Val Phe Asn Val Tyr Arg Asn Ser Tyr Phe Arg Asn Phe Lys Ile Ile 
    370                 375                 380                 


Glu Ser Asp Asp Lys Lys Gly Ile Tyr Lys Val Glu Phe Ser Thr Ser 
385                 390                 395                 400 


Asn Asn Gly Val Ile Tyr Glu Gly Tyr Ile Lys Ser Pro Ser Leu Arg 
                405                 410                 415     


Phe Ala Thr Lys Gly Gly Thr Ile Lys Ile Asp Phe Pro Ile Ser Asp 
            420                 425                 430         


Lys Arg Ile Lys Gly Gly Arg Glu Met Asn Thr Asp Leu Met Trp Phe 
        435                 440                 445             


Leu Asn Arg Ala Ser Pro Cys Ser Thr Lys Asn Lys Glu Val Asn Ser 
    450                 455                 460                 


Phe Ile Gly Lys Asn Phe Val Gly Leu Ala Ile Asp Arg Gly Ile Asn 
465                 470                 475                 480 


Pro Leu Met Ala Trp Tyr Val Ala Glu Trp Thr Tyr Asp Lys Asp Gly 
                485                 490                 495     


Lys Ala Lys Ile Val Arg Ser Ile Ala Asn Gly Arg Val Asp Ser Gly 
            500                 505                 510         


His Asn Glu Ser Glu Val Lys Phe Val Arg Glu Thr Thr Asn Arg Ile 
        515                 520                 525             


Val Gly Ile Lys Ser Leu Val Trp Asn Thr Val Lys Tyr Arg Thr Gly 
    530                 535                 540                 


Gly Ser Glu Gly Ile Asp Arg Cys Arg Lys Ser Gln Asn Gly Gln Val 
545                 550                 555                 560 


Asp Leu Phe Glu Met Phe Asp Ile Asp Tyr Asn Asn Tyr Leu Lys Glu 
                565                 570                 575     


Val Asn Asn Leu Pro Tyr Asp Pro Asn Ser Glu Arg Ser Ile Ile Gln 
            580                 585                 590         


Thr Trp Val Ser Ser Pro Trp Lys Val Lys Asp Leu Val Lys Asp Ala 
        595                 600                 605             


Lys Asn Arg Met Val Gln Ile Lys Thr Gln Tyr His Asn Ala Lys Asp 
    610                 615                 620                 


Lys Glu Lys Tyr Ile Thr Thr Gln Asn Arg Ala Gly Phe Tyr Asp Phe 
625                 630                 635                 640 


Leu Lys Ile Glu Met Glu Lys Gln Phe Thr Ser Leu Gln Arg Met Phe 
                645                 650                 655     


Ser Gly Gly Gln Lys Asp Ile Cys Lys Asn Asn Glu Glu Tyr Arg Arg 
            660                 665                 670         


Gly Leu Arg Arg Arg Ile Asn Leu Tyr Thr Ser Ser Val Ile Met Ser 
        675                 680                 685             


Leu Ala Arg Lys Phe Asn Val Asp Cys Ile Phe Leu Glu Asp Leu Asp 
    690                 695                 700                 


Ser Ser Lys Ser Ser Trp Asp Asp Ala Lys Lys Asn Ser Leu Lys Asp 
705                 710                 715                 720 


Leu Trp Ser Thr Gly Gly Ala Asp Asp Ile Leu Gly Lys Met Ala Asn 
                725                 730                 735     


Lys Tyr Lys Tyr Pro Ile Val Lys Val Asn Ser His Leu Thr Ser Leu 
            740                 745                 750         


Val Asp Asn Lys Thr Gly Lys Ile Gly Tyr Arg Asp Pro Lys Lys Lys 
        755                 760                 765             


Ser Asn Leu Tyr Val Glu Arg Gly Lys Lys Ile Glu Ile Ile Asp Ser 
    770                 775                 780                 


Asp Glu Asn Ala Ala Ile Asn Ile Leu Lys Arg Gly Ile Ser Lys His 
785                 790                 795                 800 


Ile Asp Ile Arg Glu Phe Phe Ala Glu Lys Ile Glu Val Ser Gly Lys 
                805                 810                 815     


Thr Leu Tyr Arg Ile Ser Asn Lys Leu Gly Lys Gln Arg Met Gly Ser 
            820                 825                 830         


Leu Tyr Tyr Leu Glu Gly Asn Lys Glu Ile Leu Phe Gly Leu Gly Lys 
        835                 840                 845             


Asn Gly Glu Pro Ile Val Cys Lys Arg Gly Leu Cys Lys Lys Glu Arg 
    850                 855                 860                 


Leu Ala Pro Arg Ile Ala Glu Lys Lys Ser Thr Tyr Leu Ile Met Asn 
865                 870                 875                 880 


Gly Ser Lys Trp Met Phe Arg His Glu Ala Lys Lys Ile Val Glu Thr 
                885                 890                 895     


Tyr Lys Asp Arg Tyr Cys Ala Asn His Lys Val Ala Ser Lys Asp Gly 
            900                 905                 910         


Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
        915                 920             


<210>  89
<211>  1130
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.10-NLSںϵ׵İ

<400>  89

Met Met Asn Ile Asn Glu Met Val Lys Leu Met Lys Ser Glu Tyr Leu 
1               5                   10                  15      


Phe Glu Asp Asp Gly Ile Val Thr Lys Asn Lys Ile Gln Glu Arg Leu 
            20                  25                  30          


Arg Asn Gly Phe Ser Asp Ile Gly Val Asp Pro Ser Leu Val Ser Tyr 
        35                  40                  45              


Ala Ser Lys Phe Leu Asp Ser Met Phe Ile Cys Phe Ser Arg Val Lys 
    50                  55                  60                  


Gly Glu Lys Asn Phe Lys Ala Lys Asn Val Arg Lys Asn Met Ser Ser 
65                  70                  75                  80  


Ala Glu Lys Lys Ala Gln Lys Lys Lys Glu Tyr Gln Glu Tyr Tyr Gln 
                85                  90                  95      


Gly Val Met Ala Gln Gln Asp Ala Tyr Ala Gln Leu Leu Ser Asp Pro 
            100                 105                 110         


Thr Gln Glu Asn Leu Asp Lys Leu Asn Glu Leu Ile Ser Met Ser Val 
        115                 120                 125             


Asn Gly Ser Leu Val Glu Asp Phe Phe Pro Ala Leu Lys Asn Met Ile 
    130                 135                 140                 


Gln Lys Ala Asp Tyr Ser Ile Asp Lys Lys Gly Leu Leu Asp Phe Ser 
145                 150                 155                 160 


Cys Cys Met Met Asp Arg Tyr Glu Asp Arg Ser Leu Thr Arg Ala Ile 
                165                 170                 175     


Ser Ile Ser Ala Phe Asn Ile His Ser Gly Gly Leu Arg Lys Ala Leu 
            180                 185                 190         


Ser Asp Ile Ser Glu Lys Val Gln Asp Leu Ser Asn Thr Leu Leu Ile 
        195                 200                 205             


Arg Ile Leu Tyr Met Lys Gly Glu Glu Leu Ser Ile Asp Gly Glu Lys 
    210                 215                 220                 


Ile Ser Lys Glu Glu Val Gln Arg Gln Leu Lys Ala Asp Tyr Glu Glu 
225                 230                 235                 240 


His Lys Glu Tyr Phe Glu Asp Phe Glu Asp Phe Ala Lys Lys Cys Arg 
                245                 250                 255     


Phe Phe Tyr Asn Lys Phe Ser Lys Lys Lys Lys Thr Arg Gly Phe Gly 
            260                 265                 270         


Thr Tyr Phe Phe Gly Asp Lys Lys Lys Glu Ile Ser Ser Ala Glu Tyr 
        275                 280                 285             


Lys Ala His Lys Glu Leu Arg Asp Ser Gly Tyr Leu Trp Phe Asp Ile 
    290                 295                 300                 


Gly Trp Ser Glu Ser Ser Asp Phe Lys Tyr Val Ile Val Gly Asn Val 
305                 310                 315                 320 


Ser Gly Lys Leu Lys Ser Phe Glu Glu Thr Ser Glu Glu Tyr Gln Lys 
                325                 330                 335     


Ser Lys Asn Cys Trp Glu Ala Glu Arg Val Lys Leu Tyr Glu Gln Asp 
            340                 345                 350         


Ser Asp Phe Val Leu Phe Val Glu Asp Met Ile Glu Ser Lys Tyr Gly 
        355                 360                 365             


Pro Ile Glu Lys Met Lys Leu Arg Thr Phe Lys Thr Ile Val Lys Lys 
    370                 375                 380                 


Leu Asp Lys Glu Phe Gly Lys Arg Gly Asp Lys Thr Pro Ser Ile His 
385                 390                 395                 400 


Asp Tyr Phe Glu Ser Leu Asp Pro Asn His Thr Phe Ser Gln Ser Glu 
                405                 410                 415     


Gln Phe Met Tyr Gly Leu Asp Val Thr Leu Met Gln Phe Leu Phe Asn 
            420                 425                 430         


Asn Lys Lys Gln Phe Tyr Lys Leu Cys Lys Asp His Asp Gly Lys Arg 
        435                 440                 445             


Thr Phe Ala Lys Val Val Glu Glu Ser Tyr His Trp Gly Lys Asn Ser 
    450                 455                 460                 


Ile Asn Val Ser Thr Phe Gln Asn Ser Thr Ser Ile Leu Leu Gly Gly 
465                 470                 475                 480 


Asn Tyr Leu Asn Tyr Ser Met Ser Ile Glu Gly Glu Gly Leu Val Ile 
                485                 490                 495     


Lys Phe Asp Asn Pro Leu Ser Gly Lys Glu Val His Phe Val Val Cys 
            500                 505                 510         


Asn Asn Lys Tyr Leu Ser Asp Leu Glu Ile Leu Ser Gly Asn Pro Asn 
        515                 520                 525             


Arg Lys Asp Asn Asn Tyr Thr Ile Ser Tyr Ser Thr Gly Gly Lys Ala 
    530                 535                 540                 


Arg Phe Ile Ala Lys Ser Lys Glu Pro Arg Ile Phe Phe Asn Arg Lys 
545                 550                 555                 560 


Thr Lys Lys Trp Glu Ile Ala Phe Gln Leu Ser Asp Val Ser Pro Leu 
                565                 570                 575     


Asn Gly Lys Phe Gly Lys Gln Gly Glu Phe Leu Ser Asn Leu Arg Lys 
            580                 585                 590         


Phe Val Tyr Asn His Val Ala Lys Ser Pro Ser Lys Leu Asn Ile Ser 
        595                 600                 605             


Asp Asn Asn Cys Arg Ala Val Ala Tyr Asp Leu Gly Ile Arg Asn Val 
    610                 615                 620                 


Gly Ala Trp Ser Ser Phe Asp Phe Ser Tyr Lys Asp Gly Val Leu Gly 
625                 630                 635                 640 


Gly Tyr Lys Tyr Leu Thr Ser Gly Ser Leu Arg Ser Lys Ser Glu Ser 
                645                 650                 655     


Ser Glu Met Asp Gln Gly Tyr Tyr Phe Val Leu Asn Leu Lys Lys Ile 
            660                 665                 670         


Val Lys Leu Ile Pro Val Val Lys Lys Ser Ile Ile Asp Asp Pro Glu 
        675                 680                 685             


Leu Lys Arg Gln Phe Ile Gly Val Leu Asn Glu Asn Gly Asn Thr Val 
    690                 695                 700                 


Gly Leu Gly Asn Ile Gly Lys Leu Asp Ile Ala Ser Arg Lys Ala Val 
705                 710                 715                 720 


Gln Ser Phe His Asn Cys Ile Gln Gln Ile Asn Tyr Tyr Val Asp Thr 
                725                 730                 735     


Tyr Ala Asp His Ile Asp Lys Ile Ser Ala Lys Asp Phe Val Asp Asp 
            740                 745                 750         


Ile Asp Gly Ile Lys Val Leu Asp Glu Asp Asp Pro Tyr Val Val Lys 
        755                 760                 765             


Ile Leu Ser His Leu Pro Glu Asp Val Glu Gly Asn Gln Asp Asp Ile 
    770                 775                 780                 


Leu Asn Ile Ser Leu Leu Lys Trp Lys Thr Ser Asn Ala Gln Phe Val 
785                 790                 795                 800 


Pro Pro Leu Ile Gln Glu Ala Lys Ala Ile Met Ser Arg Ile Lys Arg 
                805                 810                 815     


Glu Asn Leu Asp Asn Ile Arg Gly Lys Lys Thr Gln Val Val Thr Gln 
            820                 825                 830         


Lys Thr Phe His Lys Ile Lys Phe Ala Lys Ala Leu Leu Ser Leu Met 
        835                 840                 845             


Lys Ser Trp Ser Ser Ile Gly Thr Val Arg Val Val Lys Thr Asp Gln 
    850                 855                 860                 


Ile Tyr Gly Lys Lys Ile Trp Asp Tyr Ile Asn Gly Leu Arg Arg Asn 
865                 870                 875                 880 


Val Leu Thr Tyr Leu Ser Ser Ala Ile Val Asn Asn Ala Leu Asp Leu 
                885                 890                 895     


Gly Ala His Met Ile Ile Leu Glu Asp Leu Asp Ser Ser Val Ser Lys 
            900                 905                 910         


Tyr Arg Glu Lys Asp Lys Asn Ala Ile Gln Ser Leu Trp Gly Ser Gly 
        915                 920                 925             


Glu Leu Lys Lys Arg Ile Glu Glu Lys Ala Glu Lys His Arg Val Val 
    930                 935                 940                 


Val Gln Tyr Val Ser Pro Tyr Leu Thr Ser Gln Leu Asp Asn Glu Thr 
945                 950                 955                 960 


Lys Asp Ile Gly Tyr Arg Lys Gly Gly Arg Leu Tyr Val Val Arg Asn 
                965                 970                 975     


Gly Lys Ile Lys Ser Ile Asp Ala Asp Ile Asn Ala Ser Lys Asn Ile 
            980                 985                 990         


Gly Glu Arg Phe Phe Asp Arg Asp  Leu Ile Gln Thr Leu  Ser Gly Val 
        995                 1000                 1005             


Val Val  Glu Asp Gln Ser Thr  Val Tyr Ile Leu Gln  Lys Arg Asn 
    1010                 1015                 1020             


Val Ser  Ser Asp Asn Arg Lys  Arg Phe Tyr Lys Lys  Phe Leu Glu 
    1025                 1030                 1035             


Asp Val  Gly Gly Lys Ser Lys  Lys Asp Ala Val Leu  Lys Met Gly 
    1040                 1045                 1050             


Asp His  Gly Glu Leu Glu Val  Glu Arg Leu Ile Asp  Gly Lys Lys 
    1055                 1060                 1065             


Leu Asp  Ile Asp Gly Lys Lys  Ile Leu Val Asp Gly  Glu Lys Val 
    1070                 1075                 1080             


Pro Phe  Arg Asn Thr Ser Val  Tyr Tyr Ser Pro Lys  Lys Lys Lys 
    1085                 1090                 1095             


Trp Val  Ser Lys Glu Leu Arg  Cys Asn His Ile Lys  Leu Thr Val 
    1100                 1105                 1110             


Glu Glu  Gln Asp Ile Lys Ser  Arg Ala Asp Pro Lys  Lys Lys Arg 
    1115                 1120                 1125             


Lys Val  
    1130 


<210>  90
<211>  1146
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.11-NLSںϵ׵İ

<400>  90

Met Asn Asn Tyr Asp Asn Tyr Leu Ser Asp Tyr Leu Ala Met Leu Pro 
1               5                   10                  15      


His Thr Lys Arg Thr Glu Ile Lys Lys Thr Ala Ser Lys Ile Ser Arg 
            20                  25                  30          


Lys Leu Asn Gln Lys Glu Val Lys Lys Gln Ile Glu Arg Ser Glu Tyr 
        35                  40                  45              


Ile Arg Ser Asn Cys Gly Tyr Ile Asn Ile Glu Arg Pro Gln Lys Ser 
    50                  55                  60                  


Leu Ser Phe Leu Ser Tyr Ser Thr Ile Lys Ser Ala Cys Met Ser Val 
65                  70                  75                  80  


Asn Phe Arg Ala Phe Gln Asn Pro Ile Asn Asp Tyr Glu Thr Ala Ile 
                85                  90                  95      


Cys Asn Gly Ile Asn Glu Cys Glu Arg Phe Phe Tyr Gln Gln Ile Asp 
            100                 105                 110         


Ser Ile Tyr Met Ser Gln Ile Ile Glu Gln Leu Phe Asp Phe Tyr Ile 
        115                 120                 125             


Ala Ser Arg Gln His Asp Met Phe Ile Asn Asn Thr Val Val Pro Tyr 
    130                 135                 140                 


Asp Val Asn Lys Leu Lys Ser Tyr Tyr Thr Ala Asn Glu Lys Tyr Ser 
145                 150                 155                 160 


Phe Glu Gln Phe Cys Asp Asp Ile Lys Glu Phe Thr Asn Lys Gly Phe 
                165                 170                 175     


Thr Ser Gly Gly Val Ser Cys Ile Leu Asn Leu Phe Tyr Lys Gly Ser 
            180                 185                 190         


Val Lys Asp Ser Lys Asn Lys Lys Asp Tyr Ile Lys Ser Val Lys Arg 
        195                 200                 205             


Leu Glu Thr Asn Gly Leu Phe Lys Lys Leu Asn Ile Phe Glu Lys Asn 
    210                 215                 220                 


Gly Ile Ser Lys Tyr Phe Ala Ala Ser Thr Leu Ser Thr Phe Phe Ala 
225                 230                 235                 240 


Thr Ile Ser Ser Trp Lys Lys Gln Asn Asp Asp Trp Thr Gly Val Ala 
                245                 250                 255     


Lys Asp Gly Thr Ser Leu Leu Ser Lys Leu Glu Asn Lys Thr Ile Thr 
            260                 265                 270         


Leu Gln Ser Ile Ile Lys His His Arg Val Ile Asn Glu Leu Ala Val 
        275                 280                 285             


Leu Ile Val Lys Ala Tyr Lys Asp Pro Val Lys Thr Leu Asn Asn Leu 
    290                 295                 300                 


Phe Glu Glu Arg Ser Asp Asn Asn Asn Asp Phe Lys Tyr Thr Cys Ser 
305                 310                 315                 320 


Asp Asp Glu Asp Lys Tyr Pro Met Tyr Ile Lys Arg Glu Ile Ala Glu 
                325                 330                 335     


Phe Val Lys Lys His Lys Thr Val Trp Glu Glu Ile Arg Tyr Phe Asp 
            340                 345                 350         


Glu Ser Asp Thr Lys Lys Lys Lys Arg Asp Lys Lys Glu Ser Ser Ser 
        355                 360                 365             


Asp Asp Lys Ser Tyr Leu Cys Cys Gly Asp Ser Trp Asp Tyr Leu Lys 
    370                 375                 380                 


Thr Trp Val Arg Leu Tyr Gly Glu Tyr Tyr Phe Phe Asp Asn Ala Leu 
385                 390                 395                 400 


Asn Gln Phe Leu Arg Lys Pro Ser Ala Ser Met His Leu Tyr Thr Ser 
                405                 410                 415     


Leu Asp Trp Ile Asn Lys Lys Thr Ile Cys Ile Val Gly Ala Asn Tyr 
            420                 425                 430         


Tyr Lys Ile Gly Lys Val Glu Val Val Glu Arg Asn Asn Gln Arg Phe 
        435                 440                 445             


Leu Leu Val Tyr Val Ser Val Pro Glu Met Glu Asn Tyr Ile Ile Ile 
    450                 455                 460                 


Pro Leu Gln Leu Asn Lys Tyr Phe Gly Asn Phe Gln Cys Lys Ile Phe 
465                 470                 475                 480 


Glu Gly Arg Leu Gln Ala Ile Phe Lys Arg Tyr Ala Asn Phe Asn Ala 
                485                 490                 495     


Leu Lys Asn Asn Lys Pro Gln Pro Ser Pro Asn Ile Ser Val Arg Ile 
            500                 505                 510         


Asn Glu Phe His Phe Ala Leu Arg Ser Tyr Arg Lys Gln Gln Ile Ser 
        515                 520                 525             


Ala Glu Asp Phe Ser Lys Gly Arg Phe Ser Leu Ile Ser Lys Ile Gly 
    530                 535                 540                 


Phe Gln Met Thr Asn Asp Glu Val Phe Gly Arg Thr Pro Arg Glu Ile 
545                 550                 555                 560 


Ala Leu Val Lys Asp His Leu Ser Lys Gly Tyr Val His Phe Gly Ser 
                565                 570                 575     


Gln Ile Ile Glu Asp Ser Arg Lys Glu Val Glu Gln Val Leu Lys Lys 
            580                 585                 590         


Pro Met Ile Leu Met Gly Val Asp Phe Gly Tyr Ser Pro Leu Ala Ser 
        595                 600                 605             


Tyr Asn Ile Lys Pro Leu Gln Thr Gly Lys Pro Ala Thr Asp Trp Val 
    610                 615                 620                 


Lys Asn Leu His Gly Asn Phe Leu Cys Gln Asn Val Ser Leu Gly Glu 
625                 630                 635                 640 


Thr Ile Thr Glu Gly Glu Ile Gly Asp Val Pro Thr Asp Thr Tyr Thr 
                645                 650                 655     


Ser Ser Asn Glu Ile Tyr Ser Ile Ala Thr Leu Thr Phe Arg Asn Ala 
            660                 665                 670         


Asp Gly Lys Leu Glu Asn Arg Ser Phe Ser Arg Phe Tyr His Glu Leu 
        675                 680                 685             


Asn Asn Thr Leu Asn Ile Ile Glu Gln Ile Lys Gly Thr Phe Asn Phe 
    690                 695                 700                 


Ile His Ser Ile Asn Thr Gln Phe Lys Glu Ile Lys Ala Leu Lys Thr 
705                 710                 715                 720 


Thr Glu Glu Phe Ser Ser Tyr Val Ser Thr Leu Thr Trp Asp Gln Phe 
                725                 730                 735     


Ile Glu Asp Ser Arg Lys Thr Ala Arg Tyr Ser Lys Tyr Trp Ile His 
            740                 745                 750         


Ile Ile Asn Glu Asn Pro Lys Arg Arg Thr Ile Ala Thr Leu Asn Glu 
        755                 760                 765             


Thr Leu Lys Leu Val Asp Glu Lys His Arg Phe Thr Val Thr Ile Gln 
    770                 775                 780                 


Glu Ile Phe Asp Leu Val Lys Tyr Cys Gln Gln His Gly Tyr Tyr Pro 
785                 790                 795                 800 


Lys Ser Asn Val Met Ser Lys Leu Arg Asn Leu Ala Ile Lys Leu Ile 
                805                 810                 815     


Asn Asp Leu Ile Arg Tyr Gln Lys Ile Gly Ile His Ser Cys Tyr Leu 
            820                 825                 830         


Asp Phe Cys Val Leu Ile Lys Asn His Ile Ala Leu Leu Asn Ser Ser 
        835                 840                 845             


Thr Ala Phe Ile Ile Asn Phe Ser Arg Asn Lys Glu Asn Ile Ile Arg 
    850                 855                 860                 


Asn Asn Thr Ser Lys Ile His Ser Leu Trp Val Tyr Arg Asp Asn Phe 
865                 870                 875                 880 


Arg Arg Gln Met Ile Lys Asn Leu Cys Ser Gln Ile Leu Lys Ile Ala 
                885                 890                 895     


Ala Lys Asn Lys Val His Ile Val Val Val Glu Lys Leu Asn Asn Met 
            900                 905                 910         


Arg Thr Asn Asn Arg Asn Asn Glu Asp Lys Asn Asn Met Ile Asp Leu 
        915                 920                 925             


Leu Ala Thr Gly Gln Phe Arg Lys Gln Leu Ser Asp Gln Ala Lys Trp 
    930                 935                 940                 


Tyr Gly Ile Ala Val Val Asp Thr Ala Glu Tyr Asn Thr Ser Lys Val 
945                 950                 955                 960 


Asp Phe Met Thr Gly Glu Tyr Gly Tyr Arg Asp Glu Asn Asn Lys Arg 
                965                 970                 975     


His Phe Tyr Cys Arg Lys Gln Asp Lys Thr Val Leu Leu Asp Cys Asp 
            980                 985                 990         


Lys Lys Ala Ser Glu Asn Ile Leu  Leu Ala Phe Val Thr  Gln Ser Leu 
        995                 1000                 1005             


Leu Leu  Asn His Leu Lys Val  Leu Ile Thr Glu Asp  Gly Lys Thr 
    1010                 1015                 1020             


Ala Val  Ile Asp Leu Ser Glu  Arg Thr Thr Glu Pro  Gln Lys Ile 
    1025                 1030                 1035             


Arg Ser  Lys Ile Trp Thr Asn  Ser Asp Val Gln Lys  Ile Ile Phe 
    1040                 1045                 1050             


Cys Lys  Gln Glu Asn Gly Ser  Tyr Val Leu Lys Lys  Gly Ser Thr 
    1055                 1060                 1065             


Asp Ile  Lys Glu Lys Met His  Lys Ala Val Leu His  Arg His Gly 
    1070                 1075                 1080             


Ser Leu  Trp Tyr Asp Tyr Leu  Asn His Lys Asn Met  Ile Glu Asp 
    1085                 1090                 1095             


Ile Lys  Asn Leu His Leu Ser  Asn Cys Ser Leu Thr  Thr Ser Thr 
    1100                 1105                 1110             


Asn Ser  Asp Val Ile Asn Ser  His Ser Gly Ser Ser  Arg Ser Leu 
    1115                 1120                 1125             


Asp Lys  Thr Lys Thr Tyr Ala  Ser Arg Ala Asp Pro  Lys Lys Lys 
    1130                 1135                 1140             


Arg Lys  Val 
    1145     


<210>  91
<211>  1024
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.12-NLSںϵ׵İ

<400>  91

Met Ala Ser Ser Asp Ala Gln Lys Phe Pro Gln Thr His Asn Lys Val 
1               5                   10                  15      


Met Ser Phe Arg Leu Thr Ala Ser Asn Ile Gly Ser Val Leu Ser Leu 
            20                  25                  30          


His Ser Asn Leu His Asp Ala Ala Glu Ile Gly Ile Asn Glu Cys Arg 
        35                  40                  45              


Trp Trp Ile Gly Asp Gly Glu Ile Tyr Glu Arg Asp Pro Ala Cys Arg 
    50                  55                  60                  


Ser Ile Lys Lys Gly Asn Asp Ile Arg Thr Val Thr Ser Glu Lys Ile 
65                  70                  75                  80  


Lys Glu Leu Trp Thr Lys His Thr Asp His Ser Val Pro Leu Val Asp 
                85                  90                  95      


Phe Ile Asp Met Leu Lys Phe Val Ala Gln Cys Ala Ile Tyr Gly Asp 
            100                 105                 110         


Ser Arg Ala Leu Ala Ser Thr Leu Phe Gly Lys Ser Lys Ala Glu Thr 
        115                 120                 125             


Arg Gly Val Ser Thr Glu Asp Met Thr Val Ile Arg Ala Trp Ile Ala 
    130                 135                 140                 


Glu Thr Asp Ala Val Leu Ala Ser Gly Leu Ser Pro Lys Lys Lys Lys 
145                 150                 155                 160 


Lys Lys Glu Lys Glu Ala Gly Lys Lys Glu Arg Lys Pro Asp Val Lys 
                165                 170                 175     


Met Glu Met Cys Arg Arg Ile Arg Cys Thr Met Val Gln Cys Gly Tyr 
            180                 185                 190         


Phe Arg Arg Phe Pro Phe Glu Ala Lys Ile Asp Asn Gly Gly Glu Arg 
        195                 200                 205             


Gly Lys Met Asp Ser Glu Leu Ser Tyr Val Ser Ala Arg Asn Leu Leu 
    210                 215                 220                 


Arg Cys Leu Ser Thr Trp Arg Ala Ser Ser Val Met Arg Arg Asp Ser 
225                 230                 235                 240 


Tyr Leu Ile Glu Glu Glu Arg Ile Lys Glu Ala Glu Ser Lys Met Thr 
                245                 250                 255     


Pro Glu Ile Ile Asp Gly Leu Arg Arg Leu Tyr Arg Tyr Cys Ala Val 
            260                 265                 270         


Asp His Asp Phe Leu Lys Trp Phe Gly Gly Arg Ile Ile Arg His Ile 
        275                 280                 285             


Asp Ser Cys Leu Ala Pro Ala Ile Ala Gly Asn Thr Gly Arg Pro Thr 
    290                 295                 300                 


Gly Gly Glu Ser Phe Thr Val Ile Tyr Asp Arg Arg Lys Lys Arg Asp 
305                 310                 315                 320 


Val Lys Ile Thr Tyr Ser Val Pro Glu Glu Ile Tyr Gly Tyr Leu Ser 
                325                 330                 335     


Ser His Pro Glu Leu Val Ala Ile Gly Lys Asp Gly Met Thr Pro Ile 
            340                 345                 350         


Ser Arg His Ala Asp Tyr Leu Glu Met Ile Ala Ser His Glu Lys His 
        355                 360                 365             


Arg Trp Tyr Ala Thr Phe Pro Thr Val Gly Lys Glu Asp Gly Tyr Arg 
    370                 375                 380                 


Thr Ser Val Leu Leu Gly Lys Asn Tyr Leu Thr Tyr Asp Leu Ser Tyr 
385                 390                 395                 400 


Asp Gly Glu Ser Val Pro Asp Lys Lys Ile Asn Val Ile Ser Lys Gly 
                405                 410                 415     


Gln Pro Val Cys Leu Asp Leu His Asp Gly Arg Arg Val Ser Ser Leu 
            420                 425                 430         


Tyr Leu Thr Val Gly Glu Ser Ala Ala Tyr Asp Ile Ala Val Arg Lys 
        435                 440                 445             


Asn Lys Arg His His Gly Lys Pro Ala Asp Tyr Cys Arg Met Arg Val 
    450                 455                 460                 


His Leu Thr Gln Glu Arg Glu Asp Lys Thr Tyr Asn Asp Pro Tyr Phe 
465                 470                 475                 480 


Ser Asn Met Glu Ile Trp Arg Ala Gly Asp Gln Val Tyr Ala Ile Glu 
                485                 490                 495     


Phe Asp Arg His Gly Ala Arg Tyr Thr Ala Ile Val Lys Glu Pro Ser 
            500                 505                 510         


Val Glu Tyr Arg Asn Lys Lys Leu Tyr Leu Arg Val Asn Met Val Leu 
        515                 520                 525             


Asp Ser Pro Ser Arg Gln Asp Asp Lys Asp Met Tyr Tyr Ala Tyr Met 
    530                 535                 540                 


Thr Ala Tyr Pro Ser Ser Asn Pro Pro Val Glu Thr Ser Asp Asn Lys 
545                 550                 555                 560 


Lys Arg Phe Glu Arg Leu Gly Pro Gly Arg Arg Ala Ile Gly Gly Ile 
                565                 570                 575     


Asp Ile Gly Ile Gly Arg Pro Tyr Val Ala Val Val Ala Ser Tyr Glu 
            580                 585                 590         


Val Gly Pro Ala Gly Thr Glu Gln Lys Phe Gln Ile Glu Asp Arg Leu 
        595                 600                 605             


Ile Glu Asp Asp Gly Ser Ser Pro Tyr Asp Ser Leu Tyr Asn Asp Phe 
    610                 615                 620                 


Leu Thr Asp Ile Arg Thr Val Ser Arg Ile Ile Glu Ala Ala Lys Lys 
625                 630                 635                 640 


Ile Ser Glu Gly Asp Leu Glu Asp Ile Pro Ser Asp Met Ser Val Asp 
                645                 650                 655     


Glu Asp Gly Ser Ile Ala Ala Thr Met Lys Arg Met Ser Ala Arg Ile 
            660                 665                 670         


Ala Glu Arg His His Leu Tyr Gly Glu Arg Lys Ser Glu Ala Tyr Ala 
        675                 680                 685             


Thr Phe Leu Lys Met Asn His Lys Gln Arg Leu Asp Ile Leu Leu Thr 
    690                 695                 700                 


Gln Lys Ala Ser Asn Ala Thr Leu Lys Gln Leu Val Glu Glu Asp Pro 
705                 710                 715                 720 


Ser Phe Leu Pro Arg Ile Cys Val Tyr Tyr Val Ile Ser Val Glu Arg 
                725                 730                 735     


Glu Leu Lys Asn Lys His Arg Asn Ala Tyr Leu Asp Gly Leu Thr Val 
            740                 745                 750         


Asp Glu Lys Tyr Ser Gly Glu Thr Lys Arg Gly Tyr Ala Gln Lys Arg 
        755                 760                 765             


Leu Asn Ser Met Leu Arg Ala Tyr Ser Ala Leu Gly Glu Glu Glu Thr 
    770                 775                 780                 


Asp Glu Val Arg Thr Phe Ser Thr Arg Ser Glu Lys Val Arg Asn Met 
785                 790                 795                 800 


Ala Lys Asn Ala Ile Lys Arg Asn Ala Arg Lys Leu Val Asn Phe Tyr 
                805                 810                 815     


Val Gly Lys Gly Ile Arg Thr Ile Val Ala Glu Asp Thr Asp Pro Thr 
            820                 825                 830         


Lys Ser Arg Asn Asp Gly Lys Lys Ser Asn Arg Ile Lys Ala Ala Trp 
        835                 840                 845             


Ser Pro Lys Gln Phe Leu Ala Ala Val Lys Asn Ala Ala Gln Trp His 
    850                 855                 860                 


Gly Leu Glu Ile Ala Glu Val Asp Pro Arg Met Thr Ser Gln Val His 
865                 870                 875                 880 


Pro Glu Thr Gly Leu Ile Gly Tyr Arg Asp Gly Asp Thr Leu His Cys 
                885                 890                 895     


Pro Asp Gly Ser Lys Ile Asp Ala Asp Val Ala Gly Ala Ala Asn Val 
            900                 905                 910         


Cys Arg Val Phe Ala Gly Arg Gly Leu Trp Arg Phe Ser Ile Asn Thr 
        915                 920                 925             


Asn Ile Asp Ile Ser Asn Lys Asp Glu Lys Lys Arg Leu Arg Ala Tyr 
    930                 935                 940                 


Ile Val His His Phe Gly Ser Glu Ser Asn Trp Glu Lys Phe Arg Lys 
945                 950                 955                 960 


Gln Tyr Pro Ser Gly Thr Thr Leu Tyr Leu His Gly Arg Glu Trp Leu 
                965                 970                 975     


Thr Ala Glu Glu His Lys Ser Ala Ile Asp Arg Ile Arg Asp Asp Val 
            980                 985                 990         


Gly Arg Asp Ala Glu Asn Asp His  Val Ala Ile Val Thr  Ala Ala Glu 
        995                 1000                 1005             


Lys Val  Glu Ile Phe Ser Arg  Ala Asp Pro Lys Lys  Lys Arg Lys 
    1010                 1015                 1020             


Val 
    


<210>  92
<211>  1063
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.13-NLSںϵ׵İ

<400>  92

Met Ser His Asp Leu Lys Pro Gln Arg Leu Ile Arg Ser Asn Ile Thr 
1               5                   10                  15      


Lys Thr His Ser Asp Gln Asn Ala Lys Gln Val Ala Glu Glu Val Lys 
            20                  25                  30          


Lys Glu His Leu Asn Tyr Leu Leu Ile Lys Asn Glu Met Leu Ile Ser 
        35                  40                  45              


Ile Val Pro Glu Ala Lys Asp Asp Asp Gly Asn Asp Ile Asp Phe Lys 
    50                  55                  60                  


Lys Gln Leu Lys Ser Leu Tyr Lys Glu Thr Asp Gln Ser Val Ser Phe 
65                  70                  75                  80  


Ser Val Phe Cys Gln Met Met Lys Phe Arg Asn Ile Ala Leu Leu Tyr 
                85                  90                  95      


Ala Lys Gly Gln Ser Arg Trp Ala Val Ser Ser Tyr Phe Thr Gly Asn 
            100                 105                 110         


Arg Arg Lys Asp Asp Tyr Ala Lys Asp Leu Ser Leu Leu Asp Glu Ala 
        115                 120                 125             


Ile Glu Leu Leu Glu Cys Lys Arg Arg Lys Lys Ala Glu Glu Glu Asn 
    130                 135                 140                 


Glu Glu Glu Asn Glu Thr Pro Lys Lys Lys Glu Asp Asn Pro Ser Asn 
145                 150                 155                 160 


Ile Ser Glu Glu Gln Ile Met Lys Leu Phe Tyr Ala Val Asn Lys Lys 
                165                 170                 175     


Leu Lys Glu Ile Gly Tyr Leu Asp Arg Tyr Ser His Ile Glu Lys Gln 
            180                 185                 190         


Glu Gln Tyr Ala Ile Ile Gly Val Thr Ser Arg Thr Val Lys Ala Trp 
        195                 200                 205             


Asp Tyr Ala Asn Phe Ala Thr Arg Asn His Tyr Gln Ser Val Gln Asn 
    210                 215                 220                 


Glu Tyr Gln Lys Lys Leu Lys Ala Leu Pro Gly Thr Lys Lys Asp Lys 
225                 230                 235                 240 


Val Cys Leu Glu Lys Phe Phe Asp His Leu Asn Glu Asn Asn Ile Ala 
                245                 250                 255     


Ala Asp Trp Asp Lys Trp Arg Leu Lys Lys His Ile Leu Gln Cys Ile 
            260                 265                 270         


Ile Pro Ala Ala Lys Ile Gly Leu Lys Glu Leu Lys Gln Ser Phe Tyr 
        275                 280                 285             


Val Asp Asn Lys Gly Asn Lys His Asn Tyr Phe Val Asn Gly Leu Tyr 
    290                 295                 300                 


Glu Glu Ile Leu Lys Arg Pro Phe Leu Tyr Ser Ala Glu Asp Pro Glu 
305                 310                 315                 320 


Glu Ser Ile Leu Tyr Leu Gly Val Glu Val Ala Ser Leu His Ser Lys 
                325                 330                 335     


Leu Asn His Leu Arg Ser Glu Ala Arg Phe Ser Phe Glu Thr Pro Asp 
            340                 345                 350         


Asp Ile Cys Lys Tyr Met Thr Ile Cys Gly Asp Asn Tyr His Asn Phe 
        355                 360                 365             


Thr Met Ser Ala Ile Gly Glu Asp Val Glu Asp Ile Glu Val Glu Val 
    370                 375                 380                 


Tyr Asp Tyr Asn His Ser Lys Lys Tyr Glu Thr Met Arg Phe Ile Asn 
385                 390                 395                 400 


Gly Lys Arg Thr Thr Asp Leu Ser Leu Asn Phe Lys Gly Ile Pro Val 
                405                 410                 415     


Arg Leu Cys Leu Glu Gly Lys Arg Asn Asn Ser Tyr Phe Ala Asp Ala 
            420                 425                 430         


Ile Val Trp Glu Leu Asp Asn Lys Asp Lys Thr Gly Tyr Leu Ile Glu 
        435                 440                 445             


Tyr Gly Lys Ser Asn Asn Arg Leu Tyr Met Leu Val Lys Glu Pro Leu 
    450                 455                 460                 


Ile Gly Cys Arg Arg Lys Phe Gly Lys Asp Val Leu Phe Val Ser Leu 
465                 470                 475                 480 


Ser Gly Thr Leu Val Asn Lys Tyr Ile Glu Asp Asp Ile Val Ser Ala 
                485                 490                 495     


Arg Tyr Leu Met Gln Thr Ala Ala Pro Ile Phe Lys Thr Ser Arg Ala 
            500                 505                 510         


Lys Lys Gln Asp Lys Ile Gly Asp Lys Trp Phe Glu His Cys Gln Gly 
        515                 520                 525             


Ser Thr Ile Lys Ile Ala Gly Ile Asp Ile Gly Ile Asn Pro Ile Ala 
    530                 535                 540                 


Ala Ile Thr Val Ala Asn Val Thr Phe Asp Arg Ala Leu Gly Asn Lys 
545                 550                 555                 560 


Ile Lys Asn Gln Lys Gln Ile Val Ile Asp Cys Tyr Ala Glu Asp Tyr 
                565                 570                 575     


Lys Ile Asp Pro Val Val Val Lys Arg Met Glu Asp Ile Arg His Ile 
            580                 585                 590         


Lys Tyr Thr Ile Asn Ser Trp Tyr His Leu Ala Asp Cys Cys Arg Leu 
        595                 600                 605             


Lys Ala Ala Asn Lys Glu Tyr Val Val Asn Glu Arg Lys Gln Gly Phe 
    610                 615                 620                 


Phe Arg Glu Asn Ile Glu Tyr Leu Lys Glu Val Ala Lys Lys Ala Ile 
625                 630                 635                 640 


Thr Glu Ser Asp Gln Gln Ile Lys Glu Gln Lys Ala Ala Leu Lys Arg 
                645                 650                 655     


Phe Asp Gly Glu Lys Lys Lys Glu Ile Gln Ala Thr Ile Asn Gly Phe 
            660                 665                 670         


Asn Leu Lys Ile Lys Ile Leu Lys Lys Phe Val Arg Gln Ser Ala Lys 
        675                 680                 685             


Lys Ile Phe Asp Ser Thr Leu Glu Thr Leu Glu Lys Tyr Asp Asn Asn 
    690                 695                 700                 


Ile Glu Gln Ala Lys Arg Asp Arg Glu Phe Gly Leu Lys Ile Ile Tyr 
705                 710                 715                 720 


Asp Leu Ile Ile Lys Tyr Tyr Lys Arg Ser Lys Lys Glu Arg Glu Met 
                725                 730                 735     


Asn Gln Arg Ile Tyr Val Asp Asp Tyr Asn Gln Glu Glu Ile Asp Thr 
            740                 745                 750         


Glu Arg Thr Lys Lys Ile Arg Lys Glu Thr Ile Thr Phe Cys Asp Asn 
        755                 760                 765             


Asp Trp Asn Ser Leu Thr Lys Arg Ile His Asp Leu Glu Lys Lys Met 
    770                 775                 780                 


Lys Lys Ile Gly Ile Ser Glu Pro Gly Arg Val Glu Gln Glu Ile Asn 
785                 790                 795                 800 


Asp Arg Asp Tyr Tyr Asn Asn Ile Gln Asp Asn Thr Lys Lys Arg Gln 
                805                 810                 815     


Ala Lys Ile Ile Val Asp Ala Leu Lys Glu Glu Gly Val Ser Ile Ile 
            820                 825                 830         


Val Val Glu Asp Leu Thr Gly Gly Gly Ser Glu Asn Thr Lys Glu Ile 
        835                 840                 845             


Asn Lys Ser Phe Asp Ala Phe Ala Pro Ile Arg Phe Leu Asn Ala Leu 
    850                 855                 860                 


Lys Asn Cys Ala Glu Thr Asn Gly Ile Gln Val Thr Glu Val Leu Ser 
865                 870                 875                 880 


Pro Met Ser Ser Lys Met Val Pro Ser Thr Gly Glu Ile Gly His Arg 
                885                 890                 895     


Asp Lys Arg Asp Lys Gln Leu Tyr Tyr Lys Asp Gly Glu Glu Leu Lys 
            900                 905                 910         


Ser Ile Asp Gly Asp Ile Ser Ala Ser Glu Ile Leu Leu Arg Arg Gly 
        915                 920                 925             


Val Ser Arg His Thr Glu Leu Ile Gly Thr Met Asn Val Glu Asp Val 
    930                 935                 940                 


Leu Asp Lys Asn Asn Asn Lys Asn Lys Cys Ile Lys Gly Tyr Val Cys 
945                 950                 955                 960 


Asn Arg Trp Gly Asn Ile Gln Asn Phe Glu Lys Ile Leu Lys Glu Lys 
                965                 970                 975     


Gly Ile Gly Glu Arg Glu Ile Ile Tyr Leu His Gly Asp Lys Ile Leu 
            980                 985                 990         


Thr Met Asp Glu Lys Arg Thr Leu  Gln Ala Ser Ile Arg  Lys Glu Leu 
        995                 1000                 1005             


Lys Glu  Met Arg Glu Arg Glu  Ser Gly Glu Glu Asn  Ala Gly Thr 
    1010                 1015                 1020             


Ala Arg  Lys Lys Ser Lys Pro  Lys Lys Lys Lys Lys  Ile Lys Arg 
    1025                 1030                 1035             


Asn Asn  Asp Gln Asp Leu Ser  Asn Asn Arg Pro Ala  Ala Ser Ser 
    1040                 1045                 1050             


Arg Ala  Asp Pro Lys Lys Lys  Arg Lys Val 
    1055                 1060             


<210>  93
<211>  1056
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.14-NLSںϵ׵İ

<400>  93

Met Lys Glu Asn Lys Met Lys Glu Asn Gly Ser Met Thr Thr His Ser 
1               5                   10                  15      


Lys Val Ile Ala Leu Lys Met Lys Ser Glu Asn Val Glu Phe Asp Thr 
            20                  25                  30          


Phe Tyr Lys Glu Ser Phe Glu Leu Phe Lys Gln Phe Thr Asn Glu Phe 
        35                  40                  45              


Val Ala Trp Gly Asn Asp Glu Ile Tyr Gln Tyr Gly Ser Ser Lys Arg 
    50                  55                  60                  


Lys Lys Asp Asp Gln Lys Ile Ser Leu Ile Pro Val Ile Glu Asp Ile 
65                  70                  75                  80  


Tyr Lys Ser Val Glu Lys Lys Ala Thr Ala Glu Gly Ile Ser Lys Thr 
                85                  90                  95      


Asp Phe Arg Ala Val Leu Lys Tyr Leu Tyr His Gln Ile Ile Asn Val 
            100                 105                 110         


Gly Asn Ser Gly Arg Ser Tyr Gly Thr Ser Leu Phe Gly Gly Cys Glu 
        115                 120                 125             


Val Lys Glu Lys Leu Ser Lys Gln Asp Ile Ser Asn Ile Val Glu Cys 
    130                 135                 140                 


Val Lys Glu Leu Glu Leu Cys Lys Ser Lys Gln Glu Glu Ser Asp Ala 
145                 150                 155                 160 


Tyr Asp Lys Ile Leu Leu Lys Glu Lys Ile Thr His Ile Val Lys Ser 
                165                 170                 175     


Gly Glu Thr Ala Gly Asp Ile Thr Lys Lys Tyr Asn Gln Ala Thr Thr 
            180                 185                 190         


Gly Arg Lys Thr Ser Ser Lys Gly Phe Phe Asp Lys Ser Thr Lys Thr 
        195                 200                 205             


Glu Val Lys Tyr Lys Asp Ile Lys Asp Asp Thr Leu Leu Gln Asp Gly 
    210                 215                 220                 


Ser Thr Ile Phe Ile Lys Ser Ser Val Asp Leu Phe Val Lys Lys Val 
225                 230                 235                 240 


Cys Asn Thr Leu Arg Glu Ile Asn Phe Phe Asp Arg Leu Pro Phe Lys 
                245                 250                 255     


Asn Asn His Ser Asn Asn Tyr Gly Leu Leu Phe Ser Met Leu Ser Gln 
            260                 265                 270         


Ile Glu Ser Trp Lys Thr Ile Ser Glu Thr Thr Lys Lys Ser His Glu 
        275                 280                 285             


Glu His Gly Glu Lys Ile Ala Ser Met Val Lys Lys Leu Asp Leu Thr 
    290                 295                 300                 


Gln Thr Glu Leu Met Lys Asp Phe Ala Ala Phe Cys Ile Glu Asn Asn 
305                 310                 315                 320 


Ile Thr Lys Lys Phe Asp His Lys Phe Lys Arg His Met Glu Asp Cys 
                325                 330                 335     


Val Ile Pro Ser Phe Lys Asn Gly Lys Ile Pro Asp Lys Leu Phe Tyr 
            340                 345                 350         


Phe Asn Ile Ile Leu Ala Lys Lys Thr Asp Glu Gln Ile Asp Tyr Ser 
        355                 360                 365             


Leu Ser Ser Glu Phe Tyr Thr Lys Leu Phe Ser Met Pro Asn Leu Trp 
    370                 375                 380                 


Gln Glu Glu Glu Ala Phe Ile Val Lys Asn Ile Asn Leu Ile Glu Glu 
385                 390                 395                 400 


Ile Thr Ile Phe Asn Lys Arg Arg Asn Tyr Ala Cys Cys Pro Leu Ile 
                405                 410                 415     


Lys Glu Lys Glu Tyr Asp Arg Phe Gln Ile Gln Leu Asn Glu Thr Asn 
            420                 425                 430         


Phe Leu Lys Phe Gln Phe Asp Pro Lys Asn Val Val Asn Ile Asp Glu 
        435                 440                 445             


Asn Thr Thr Glu Ala Thr Val Gly Phe Asp Glu Lys Leu Lys Leu Val 
    450                 455                 460                 


Val Cys Ala Asp Lys Lys Tyr Ala Phe Ser Ile Phe Thr Gln Cys Lys 
465                 470                 475                 480 


Tyr His Gly Asn Lys His Lys Pro Asn Thr Tyr Phe Asn Asn Leu Lys 
                485                 490                 495     


Ile Ile Lys Val Ile Glu Ser Lys Ser Asn Ser Val Lys Ser Met Lys 
            500                 505                 510         


Tyr Thr Phe Glu Phe Thr Lys Arg Asn Glu Leu Lys Arg Ala Glu Ile 
        515                 520                 525             


Lys Gln Pro Ser Ile Val Tyr Lys Asn Asn Asn Tyr Tyr Ile Arg Ile 
    530                 535                 540                 


Asn Met Asn Val Ile Leu Asp Ala Asp Gln Thr Ser Tyr Lys Ile Ile 
545                 550                 555                 560 


Asn Asn Asn Gln Thr Ala Ser Leu Pro Ser Tyr Phe Gln Ser Ser Leu 
                565                 570                 575     


Pro Phe Glu Asn Asn Arg Gly Lys Ile His Asp Lys Gly Ile Val His 
            580                 585                 590         


Trp Glu Lys Ile Lys Asn Arg Lys Ile Ile Ala Met Gly Val Asp Leu 
        595                 600                 605             


Gly Val Arg Arg Pro Phe Ser Tyr Ala Ile Gly Asn Phe Thr Leu Asn 
    610                 615                 620                 


Lys Asp Ile Leu Asp Lys Asn Asp Val Asn Ile Val Ala Ser Gly Phe 
625                 630                 635                 640 


Asn Leu Cys Ser Asp Ser Asp Val Tyr Phe Gln Val Phe Asn Gln Ile 
                645                 650                 655     


Lys Thr Leu Ala Lys Phe Ile Gly Lys Leu Lys Ser His Asn Lys Gly 
            660                 665                 670         


Leu Lys Val Asp Phe Glu Lys Asp Lys Lys Tyr Ile Phe Asp Leu Val 
        675                 680                 685             


Asn Asp Ala Lys Ala Tyr Phe Lys Asp Met Ser Ala Lys Arg Ile Asn 
    690                 695                 700                 


Asp Thr Lys Asp Asn Ile Ser Asn Thr Val Thr Asn Lys Glu Arg Ile 
705                 710                 715                 720 


Tyr Gly Ser Phe Val Ser Glu Ser Ala Glu Ser Ala Ile Gln Cys Ala 
                725                 730                 735     


Ile Asp Arg Ser Glu Lys Glu Ser Gly Leu Thr Leu Lys Lys Asp Ile 
            740                 745                 750         


Ser Trp Leu Val Asn Val Leu Ser Lys Tyr Leu Glu Arg Lys Phe Lys 
        755                 760                 765             


Glu Val Lys Asn Asn Arg Lys Tyr Thr Asn Val Asn Lys Cys Asp Asn 
    770                 775                 780                 


Cys Phe Asn Trp Leu Arg Val Ile Glu Asn Ile Lys Arg Leu Lys Arg 
785                 790                 795                 800 


Ser Ile Ser Tyr Leu Gly Glu Asp Leu Gln Lys Asn Pro Glu Leu Lys 
                805                 810                 815     


Ile Glu Leu Lys Asn Leu Asn Glu Tyr Gly Asn Asn Val Lys Ser Asp 
            820                 825                 830         


Phe Leu Lys Gln Ile Ala Ser Asn Ile Ile Lys Val Ala Ile Glu His 
        835                 840                 845             


Lys Cys Asp Ile Val Phe Ile Glu Lys Leu Gly Lys Ala Asp Ser Arg 
    850                 855                 860                 


Ser Arg Lys Leu Asn Glu Met Phe Ser Phe Trp Ser Pro Lys Ala Ile 
865                 870                 875                 880 


Lys Lys Ala Ile Glu Asn Ala Ala Ser Trp His Gly Ile Pro Val Val 
                885                 890                 895     


Glu Val Asp Pro Ser Cys Thr Ser Lys Val His Tyr Glu Thr Asn Leu 
            900                 905                 910         


Phe Gly His Arg Ile Gly Asn Asp Leu Tyr Tyr Val Glu Asp Gln Cys 
        915                 920                 925             


Leu Lys Lys Val Asp Ala Asp Ile Asn Ala Ala Lys Gln Ile Leu Val 
    930                 935                 940                 


Arg Gly Ala Thr Arg His Gly Asn Ile Ser Ser Ile Asn Ile Lys Tyr 
945                 950                 955                 960 


Leu Gln Ala Lys Ile Ala Glu Leu Asn Ser Glu Ala Asn Ser Glu Glu 
                965                 970                 975     


Asp Lys Glu Glu Ile Lys Gln Gly Gly Lys Arg Ile Gln Gly Phe Leu 
            980                 985                 990         


Trp Lys Lys Tyr Gly Asn Ile Thr  Asn Ile Thr Asn Gln  Leu Thr Ala 
        995                 1000                 1005             


Ala His  Lys Glu Arg Glu Ser  Lys Phe Asp Tyr Ile  Tyr Leu His 
    1010                 1015                 1020             


Asn Asp  Lys Trp Ile Ala Tyr  Glu Asp Arg Asn Glu  Ile Lys Lys 
    1025                 1030                 1035             


Asp Ile  Glu Lys Arg Leu Glu  Ser Arg Ala Asp Pro  Lys Lys Lys 
    1040                 1045                 1050             


Arg Lys  Val 
    1055     


<210>  94
<211>  906
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.15-NLSںϵ׵İ

<400>  94

Met Thr Ala Lys Lys Thr Ala Lys Lys Tyr Phe Pro Pro Lys Cys Leu 
1               5                   10                  15      


Arg Ser Ser His Phe Lys Ile Tyr Gly Ile Pro Thr Ala Ile Arg Ala 
            20                  25                  30          


Leu Glu Glu Thr Asn Thr Phe Val Asn Lys Ala Ala Ala Asp Leu Met 
        35                  40                  45              


Glu Met Phe Phe Leu Met Arg Gly Gln Pro Tyr Arg Arg Arg Ile Gly 
    50                  55                  60                  


Ser Glu Glu Lys Gln Val Thr Gln Glu His Ile Asp Ala Arg Leu Arg 
65                  70                  75                  80  


Val Leu Val Gly Asp Tyr Ser Leu Asn Glu Val Lys Pro Leu Leu Arg 
                85                  90                  95      


Gln Leu Tyr Asp Gly Ile Lys Ala Lys Gln Asn Tyr Ala Pro Thr His 
            100                 105                 110         


Phe Val Arg Phe Phe Ile Gln Pro Thr Lys Gly Ala Ile Asp Lys Lys 
        115                 120                 125             


Ser Pro Val Ser Gln Arg Ala Lys Lys Ala Gly Gln Lys Leu Gln Lys 
    130                 135                 140                 


Met Gly Val Leu Pro Ile Leu Pro Leu Ser Pro Gly Phe Lys Phe Trp 
145                 150                 155                 160 


Thr Ala Ala Met Met Met Ala Cys Ser Arg Met Asn Ser Trp Glu Ala 
                165                 170                 175     


Cys Asn Glu Lys Thr Ile Glu Asn His Lys Ala Phe Leu Glu Gly Ile 
            180                 185                 190         


Glu Asn Tyr Lys Lys Glu Ile Arg Phe Glu Asp Leu Cys Glu Glu Trp 
        195                 200                 205             


Ser Leu Phe Ser Asp Trp Leu Thr Glu Ala Glu Ser Asp Asn Glu Gly 
    210                 215                 220                 


Gly Cys Lys Phe Lys Leu Thr Pro Arg Phe Leu Gln Arg Trp Glu Arg 
225                 230                 235                 240 


Ile Tyr Leu Lys Gln Met Arg Lys Gly Lys Ile Pro Ala Arg His Asn 
                245                 250                 255     


Leu Gly Pro Val Met Glu Ala Leu Ala Gly Asp Lys Tyr Arg Gln Leu 
            260                 265                 270         


Trp Asp Asn Gly Glu Glu Arg Asp Tyr Ile Thr Glu Leu Gly Asp Leu 
        275                 280                 285             


Val Thr Ser Gln Arg Lys Ala Val Arg Leu Ser Arg Asp Ser Ala Val 
    290                 295                 300                 


Thr Phe Pro Asp Glu Glu Leu Ser Pro Val Gly Thr Glu Phe Gly His 
305                 310                 315                 320 


Asn Tyr Met Ser Phe Ser Ile Asp Gln Glu Asn Ser His Leu Val Thr 
                325                 330                 335     


Leu Glu Val Ile Gly Gly Lys Tyr Gln Phe Glu Ile Ser Lys Ser Asp 
            340                 345                 350         


Tyr Phe Arg Asp Leu Ile Val Glu Glu Ala Gly Lys Gln Ser Lys Phe 
        355                 360                 365             


Tyr Asn Val Ser Tyr Arg Lys Gly Asn Val Arg Glu Glu Asn Leu Ala 
    370                 375                 380                 


Gly Asp Phe Lys Glu Ala Thr Val Arg Asn Arg Arg Ser Leu Lys Thr 
385                 390                 395                 400 


Gly Lys Arg Arg Leu Tyr Phe Tyr Met Ser His Ser Ile Pro Thr Arg 
                405                 410                 415     


Phe Asp Asp Asp Leu Tyr Ala Gln Phe Thr Glu Lys Gly Gln Pro Asp 
            420                 425                 430         


Phe Ser Lys Leu Tyr Lys Ala Val Thr Tyr Phe Gln Cys Ser Leu Gly 
        435                 440                 445             


Asn Lys Lys Ala Asp Thr Tyr Arg Val Tyr Val Lys Met Gly Thr Arg 
    450                 455                 460                 


Phe Leu Gly Val Asp Ile Gly Val Ser Arg Leu Phe Gly Phe Ser Leu 
465                 470                 475                 480 


Phe Glu Leu Arg Glu Glu Lys Pro Glu Lys Asn Pro Phe Phe Glu Leu 
                485                 490                 495     


Pro Asp Asp Leu Gly Tyr Ala Val Cys Leu Glu Ser Trp Val Asp Gly 
            500                 505                 510         


Val Glu Lys Asn His Lys Val Ala Gln Glu Met Lys Asp Trp Arg Arg 
        515                 520                 525             


Glu Cys Leu Ala Ala Gln Arg Leu Ile His Tyr Ala Lys Phe Leu Lys 
    530                 535                 540                 


Lys Arg Asp Lys Asn Glu Glu Ile Asp Tyr Lys His Glu Glu Ser Leu 
545                 550                 555                 560 


Glu Thr Ile Ala Gly Leu Leu Gly Ile Glu Ile Asp Pro Glu Gln Ile 
                565                 570                 575     


Ile Asp Val Pro Leu Lys Leu Leu Asp Leu Val Gly Gln Ala Ile Gly 
            580                 585                 590         


Ala Leu Arg Lys Lys Tyr Leu Val Leu Lys Lys Asn Glu Val Arg Gln 
        595                 600                 605             


Gly Arg Ile Thr Ser Glu Leu Phe Leu Trp Pro Glu Cys Val Asp Thr 
    610                 615                 620                 


Tyr Ile Arg Leu Leu Lys Ser Trp Thr Tyr Lys Asp Lys Lys Pro Tyr 
625                 630                 635                 640 


Gln Lys Gly Glu Thr Asn Lys Asp Ala Phe Lys Lys Leu Lys Gly Tyr 
                645                 650                 655     


Leu Ala Arg Leu Arg Lys Asp Leu Ala Pro Lys Tyr Ala Ala Val Ile 
            660                 665                 670         


Ala Asp Ala Ala Ile Arg His Lys Val His Val Val Val Ala Glu Asn 
        675                 680                 685             


Leu Glu Gln Phe Gly Leu Ser Met Lys Asn Glu Lys Asp Leu Asn Arg 
    690                 695                 700                 


Val Leu Ala His Trp Ser His Gln Lys Ile Trp Ser Met Val Glu Glu 
705                 710                 715                 720 


Gln Leu Arg Pro Tyr Gly Ile Met Val Val Tyr Val Asp Pro Arg His 
                725                 730                 735     


Thr Ser Lys Leu Asp Phe Ala Thr Asp Glu Phe Gly Gly Arg Cys Phe 
            740                 745                 750         


Thr Ser Leu Tyr Val Met Arg Asp Gly Lys Lys Thr Thr Thr Asp Thr 
        755                 760                 765             


Glu Lys Asn Ala Ser Gln Asn Ile Pro Lys Lys Phe Leu Thr Arg His 
    770                 775                 780                 


Arg Asn Val Ser Trp Leu Leu Ala Tyr Ala Val Asp Leu Ser Asp Ser 
785                 790                 795                 800 


Gln Lys Lys Lys Leu Gly Ile Gly Asp Glu Lys Val Trp Leu Pro Asn 
                805                 810                 815     


Met Gly Leu Met Ile Ser Gly Ala Leu Lys Ala Lys His Gly Lys Asn 
            820                 825                 830         


Ser Ala Leu Leu Val Glu Asp Gly Glu Asn Tyr Arg Leu Leu Pro Ile 
        835                 840                 845             


Thr Ala Ala Gln Ala Lys Lys Phe Val Val Lys Arg Lys Lys Glu Glu 
    850                 855                 860                 


Phe Tyr Arg His Gly Glu Ile Trp Leu Thr Lys Glu Ala His Lys Ala 
865                 870                 875                 880 


Arg Ile Glu Tyr Leu Phe Pro Glu Ser Lys Lys Gly Arg Lys Ser Ser 
                885                 890                 895     


Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
            900                 905     


<210>  95
<211>  967
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.16-NLSںϵ׵İ

<400>  95

Met Lys Lys Thr Asn Tyr Lys Thr Ser His Leu Leu Ile Asp Asn Pro 
1               5                   10                  15      


Pro Gln Ser Ile Ile Asp Leu His Arg Asp Val Ile Glu Ile Gly Ser 
            20                  25                  30          


Tyr Leu Thr Lys Phe Phe Leu Ala Cys Leu Gly Arg Pro Val Asp Ser 
        35                  40                  45              


Thr Ile Leu Ser Glu Pro Ala Leu His Phe Gln Phe Val Asn Gly Ile 
    50                  55                  60                  


Leu Pro Val Lys Asn Gly Pro Gly Ala Asp Asp Ser Ser Trp Arg His 
65                  70                  75                  80  


Ser Glu Asn Cys Tyr Ser Met Leu Phe Glu Lys Asn Ser Lys Ser Gly 
                85                  90                  95      


Lys Ser Asp Gly Lys Val Arg Gln Val Arg Glu Leu Lys Val Ala Leu 
            100                 105                 110         


Phe Gly Lys Lys Glu Lys Gly Lys Gly Ile Val Gly Lys Lys Thr Trp 
        115                 120                 125             


Asp Glu Leu Lys Val Val Leu Glu Ala Leu Pro Glu Glu His Gln Ile 
    130                 135                 140                 


Leu Ser Leu Glu Ile Cys Gln Arg His Tyr Glu Ser Arg Asp Val Lys 
145                 150                 155                 160 


Ala Phe Gly Lys Leu Ala Leu Ser Ser Lys Ser Arg Pro Ser Val Glu 
                165                 170                 175     


Ala Gly Leu Lys Leu Arg Glu Leu Gly Leu Leu Pro Leu Asp Ser Arg 
            180                 185                 190         


Gly Leu Asp Lys Asn Lys Leu Leu Gly Ile Leu Ala Ala Val Thr Gly 
        195                 200                 205             


Arg Leu Lys Ser Trp Arg Asp Arg Asp Cys Ala Cys Lys Ala Asp Lys 
    210                 215                 220                 


Gln Ala Leu Arg Val Lys Phe Glu Glu Arg Leu Ser Lys Val Asp Gln 
225                 230                 235                 240 


Ser Ala Tyr Gln Gln Phe Lys Gln Phe Ala Asp Glu Leu Leu Thr Gln 
                245                 250                 255     


Glu Gly Tyr Arg Ile Ser Gly Arg Val Leu Arg Ala Val Glu Lys Lys 
            260                 265                 270         


Asp Ser Asp Tyr Ser Pro Val Leu Thr Val Leu Ala Lys Tyr Pro Asp 
        275                 280                 285             


Leu Gln Asp Asn Phe Glu Glu Leu Cys Arg Ala Cys Leu Ala Glu Gln 
    290                 295                 300                 


Ala Phe Asn Lys Lys Lys Ala Asp Ala Arg Val Thr Val Cys Ser Glu 
305                 310                 315                 320 


Thr Ser Pro Leu Gln Phe Pro Phe Gly Met Thr Gly Asn Gly Tyr Pro 
                325                 330                 335     


Phe Thr Leu Ser Ala Cys Glu Gly Arg Ile Asn Ala Thr Ile His Phe 
            340                 345                 350         


Pro Gly Gly Asp Leu Pro Leu Arg Leu Arg Lys Ser Lys Tyr Phe Gln 
        355                 360                 365             


Asn Pro Glu Ile Leu Pro Val Lys Asp Gly Phe Gln Ile Thr Phe Thr 
    370                 375                 380                 


Arg Gly Lys Thr Pro Leu Val Gly Thr Ile Lys Glu Pro Ser Leu Leu 
385                 390                 395                 400 


Lys Lys Asn Asn His Tyr Tyr Leu Ser Leu Arg Val Asn Val Pro Ser 
                405                 410                 415     


Val Lys Ile Pro Lys Glu Val Arg Asp Thr Arg Ala Tyr Tyr Ser Ser 
            420                 425                 430         


Ala Val Gly Gly Asp Glu Thr Thr Pro Val Pro Val Lys Ala Val Ala 
        435                 440                 445             


Ile Asp Leu Gly Val Thr Thr Leu Ala Asp Tyr Ser Ile Ile Asp Thr 
    450                 455                 460                 


Cys Leu Pro Gly Asp Cys Lys Val Phe Gly Gly Glu Thr Ala Ala Phe 
465                 470                 475                 480 


Thr Ala His Gly Lys Ile Gly Gln Cys Ala Asn Lys Ser Leu Arg Asp 
                485                 490                 495     


Arg Leu Tyr Lys Asn Thr Glu Glu Ala Leu Phe Leu Gly Lys Phe Ile 
            500                 505                 510         


Arg Leu Ser Lys Lys Leu Arg Asp Gly Glu Gly Leu Asn Arg Trp Glu 
        515                 520                 525             


Val Glu Lys Leu Pro Gly Tyr Ala Glu Arg Leu Gly Ile Thr Gln His 
    530                 535                 540                 


Leu Asp Asn Ala Tyr Thr Arg Lys Asp Glu Ile Ala Arg Lys Phe Lys 
545                 550                 555                 560 


Gln Ile Lys Gly Asn Phe Asp Lys Leu Val Ser Glu Phe Ala Leu Arg 
                565                 570                 575     


Asp His Pro Ser Lys Lys Gly Glu Ser Trp Glu Thr Ile Ser Ala Glu 
            580                 585                 590         


Thr Ile Gln Val Leu Ala Ala Leu Lys Arg Ile Gln Ser Leu Leu Lys 
        595                 600                 605             


Ser Trp Thr Tyr Tyr Ser Trp Thr Ala Glu Asp Tyr Val Leu Ala Leu 
    610                 615                 620                 


Thr Ala Asp Gly Pro Val Cys Ile Asp Gly Glu His Val Lys Ala Val 
625                 630                 635                 640 


Thr Ala Thr Ser Arg Arg Ser Phe Ala Pro Cys Gly Lys Ala Ala Leu 
                645                 650                 655     


Leu Arg Leu Ile Glu Ser Gly Glu Ile Val Glu Thr Gly Gly Gln Tyr 
            660                 665                 670         


Gln Leu Ala Thr Gly Val Lys His Arg Asn His Pro Val Asn Phe Leu 
        675                 680                 685             


Ser Ser Tyr Ile Lys His Phe Asn Gly Leu Arg Arg Asp Leu Thr Asn 
    690                 695                 700                 


Lys Leu Val Arg Ala Ile Val Asn Lys Ala Gln Glu Tyr Arg Val Gln 
705                 710                 715                 720 


Ile Val Ile Val Glu Asp Phe Gly Ile Ala Asp Leu Glu Asp Arg Ile 
                725                 730                 735     


Lys Asp Ala Tyr Glu Asn Tyr Arg Trp Asn Leu Phe Ala Pro Ala Thr 
            740                 745                 750         


Ile Val Lys Lys Leu Glu Ala Ala Leu Leu Glu Val Gly Ile Ala Met 
        755                 760                 765             


Ala Gln Val Asp Pro Arg His Thr Ser Gln Ile Ala Pro Thr Gly Ala 
    770                 775                 780                 


Phe Gly Phe Arg Asp His Ala Phe Leu Tyr Tyr Gln Asp Asp Gly Leu 
785                 790                 795                 800 


Cys Arg Ile Asp Ala Asn Thr Asn Ala Ser Met Arg Ile Ala Glu Arg 
                805                 810                 815     


Phe Phe Met Arg His Ser Val Leu Thr Gln Leu Arg Ala Ala Lys Ile 
            820                 825                 830         


Gly Glu Thr Glu Tyr Leu Ile Pro Glu Ser Ala Ser Lys Arg Leu Asn 
        835                 840                 845             


Ala Phe Val Lys Leu Gln Thr Gly Lys Pro Phe Ala Lys Leu Ile Met 
    850                 855                 860                 


Asn Cys Ser Gly Phe Val Leu Glu Gly Leu Thr Lys Lys Gln Tyr Ala 
865                 870                 875                 880 


Lys Leu Ala Glu Thr Ala Gly Lys Lys Glu Ser Phe Tyr Gln Tyr Asp 
                885                 890                 895     


Asp Arg Trp Phe Asp Lys Gly His His Phe Ala Cys Arg Ala Thr Leu 
            900                 905                 910         


Glu Asn Lys Val Gln Val Cys Leu Asn Gly Gly Gly Arg Ile Lys Asp 
        915                 920                 925             


Thr Thr Pro Asp Phe Asn Pro Lys Ser Leu Leu Arg Ser Asp Leu Gln 
    930                 935                 940                 


Thr Pro Leu Asp Gln Leu Phe Gly Asn Ser Gly Ala Ser Arg Ala Asp 
945                 950                 955                 960 


Pro Lys Lys Lys Arg Lys Val 
                965         


<210>  96
<211>  957
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.17-NLSںϵ׵İ

<400>  96

Met Ser Asn Thr Thr Tyr Lys Thr Ser His Leu Leu Ile Asp Leu Pro 
1               5                   10                  15      


Gln Gln Glu Leu Ile Asp Leu His Arg Asp Ser Asn Glu Met Gly Ser 
            20                  25                  30          


Tyr Leu Thr Lys Phe Phe Leu Ala Ala Leu Gly Arg Pro Val Asp Asn 
        35                  40                  45              


Ser Ile Val Leu Pro Pro Glu Leu Ala Asp Leu Tyr Phe Gln Phe Ala 
    50                  55                  60                  


Asn Gly Ile Leu Pro Val Asp Lys Gly Pro Gly Ser Asp Asp Pro Ser 
65                  70                  75                  80  


Trp Leu His Ser Glu Asn Cys Tyr Ser Met Phe Phe Glu Lys Asp Ser 
                85                  90                  95      


Met Ser Gly Asn Cys Thr Asn Lys Ile Lys Gln Tyr Gln Glu Leu Lys 
            100                 105                 110         


Thr Ala Leu Cys Gly Gln Lys Val Lys Gly Gln Lys Gly Leu Val Gly 
        115                 120                 125             


Lys Lys Thr Trp Ala Gln Leu Lys Lys Val Leu Thr Ala Leu Pro Gln 
    130                 135                 140                 


Lys Tyr Gln Ile Leu Ser Pro Lys Ile Cys Gln Lys Tyr Phe Lys Ser 
145                 150                 155                 160 


Gly Asn Leu Glu Gly Phe Gly Lys Leu Ala Leu Ala Gly Lys Asn Arg 
                165                 170                 175     


Pro Ser Met Ser Ala Gly Leu Gln Leu Arg Glu Leu Gly Leu Leu Pro 
            180                 185                 190         


Leu Asp Ser Arg Gly Ile Asp Lys Asn Lys Leu Leu Gly Ile Leu Val 
        195                 200                 205             


Gly Ile Thr Gly Arg Leu Lys Ser Trp Arg Asp Arg Asp Trp Ala Cys 
    210                 215                 220                 


Lys Thr Val Lys Glu Glu Leu Arg Val Thr Phe Glu Lys Gly Leu Gly 
225                 230                 235                 240 


Glu Val Asp Pro Thr Ala Tyr Pro Gln Phe Lys Gln Phe Ala Asp Gln 
                245                 250                 255     


Leu Phe Lys Gln Glu Gly Tyr Lys Ile Ser Gly Arg Val Leu Arg Ala 
            260                 265                 270         


Val Glu Gly Lys Asp Ala Asp Tyr Gln Pro Val Leu Ser Leu Leu Thr 
        275                 280                 285             


Gln Tyr Pro Asp Leu Gln Gly Asp Phe Glu Glu Leu Gly Arg Val Tyr 
    290                 295                 300                 


Leu Ala Glu Ala Glu Tyr Leu Arg Lys Lys Val Asp Ala Arg Val Thr 
305                 310                 315                 320 


Val Cys Asp Ala Glu Thr Ser Pro Leu Gln Phe Pro Phe Gly Leu Thr 
                325                 330                 335     


Gly Asn Gly Tyr Ser Ile Thr Leu Thr Val Val Lys Gly Gln Ile Ala 
            340                 345                 350         


Ala Thr Leu His Leu Pro Gly Gly Asp Ile Thr Pro Arg Leu Arg Arg 
        355                 360                 365             


Ser Lys Tyr Phe Gln Asn Pro Glu Ile Ala Pro Val Lys Asp Gly Lys 
    370                 375                 380                 


Gly Lys Val Asn Gly Phe Gln Ile Ser Phe Lys Arg Gly Lys Thr Pro 
385                 390                 395                 400 


Leu Val Gly Ile Ile Lys Glu Pro Lys Leu Leu Lys Lys Asn Gly Asn 
                405                 410                 415     


Tyr Tyr Leu Ser Leu Ala Val Gly Ile Asn Lys Thr Glu Ile Pro Lys 
            420                 425                 430         


Glu Ile Cys Asp Ala Arg Ala Tyr Tyr Ser Ser Thr Ser Arg Thr Asp 
        435                 440                 445             


Thr Pro Pro Ala Val Lys Ala Met Ser Ile Asp Leu Gly Val Thr Thr 
    450                 455                 460                 


Leu Ala Asp Tyr Ser Ile Ile Asp Thr Gly Leu Pro Gly Asp Cys Gly 
465                 470                 475                 480 


Val Phe Gly Gly Ser Thr Ala Ala Phe Thr Glu His Gly Lys Ile Gly 
                485                 490                 495     


Arg Cys Gly Ser Lys Ser Leu Arg Asp Gly Leu Tyr Lys Asn Thr Glu 
            500                 505                 510         


Ala Gly Tyr Phe Leu Ala Lys Tyr Ile Arg Leu Ser Lys Asn Leu Arg 
        515                 520                 525             


Gly Gly Val Gly Leu Asn Lys Leu Glu Lys Glu Lys Leu Leu Glu His 
    530                 535                 540                 


Val Glu Arg Leu Gly Ile Glu His Cys Ala Asp Asp Phe Ala Arg Lys 
545                 550                 555                 560 


Asp Glu Ile His Arg Lys Phe Ser Glu Ile Lys Ser Lys Leu Glu Lys 
                565                 570                 575     


Ser Ile Ser Glu Phe Ala Leu Arg Asp Arg Pro Asp Lys Lys Gly Ala 
            580                 585                 590         


Ser Trp Glu Gly Ile Cys Ala Glu Thr Val Gln Val Leu Gly Ala Val 
        595                 600                 605             


Lys Arg Trp Gln Ser Leu Ala Lys Ser Trp Thr Tyr Tyr Ser Trp Thr 
    610                 615                 620                 


Ala Glu Asp Tyr Val Leu Ala Leu Thr Gly Glu Gly Arg Thr Arg Val 
625                 630                 635                 640 


Ser Asp Glu His Val Glu Ser Val Val Lys Thr Gly Arg Arg Gln Phe 
                645                 650                 655     


Ala Pro Cys Gly Lys Ala Ala Leu Leu Arg Leu Leu Glu Lys Gly Lys 
            660                 665                 670         


Ile Val Glu Val Cys Pro Gly Gln Phe Gln Leu Ala Glu Gly Val Asp 
        675                 680                 685             


Tyr Lys Arg His Pro Thr Glu Phe Leu Ala Ala His Ile Arg His Phe 
    690                 695                 700                 


Asn Gly Leu Arg Arg Asp Leu Thr Asn Lys Leu Val Arg Ala Ile Val 
705                 710                 715                 720 


Glu Lys Ala Gln Gln His Arg Val Gln Ile Val Ile Val Glu Asp Phe 
                725                 730                 735     


Gly Ile Pro Asp Ile Glu Gly Arg Ile Met Asp His Tyr Asp Asn Tyr 
            740                 745                 750         


Arg Trp Asn Leu Phe Ala Pro Ala Lys Val Ile Glu Lys Leu Glu Glu 
        755                 760                 765             


Ala Leu Ser Glu Val Gly Ile Ala Met Ala Glu Val Asp Pro Arg His 
    770                 775                 780                 


Thr Ser Gln Leu Ala Pro Thr Gly Asp Phe Gly Phe Arg Asp His Glu 
785                 790                 795                 800 


Asn Leu Tyr Phe Trp Glu Lys Gly Leu Cys Arg Thr Asp Ala Asn Thr 
                805                 810                 815     


Asn Ala Ser Met Arg Ile Ala Glu Arg Phe Phe Thr Arg His Ser Val 
            820                 825                 830         


Leu Ser Gln Leu Arg Ala Val Lys Ile Ser Glu Thr Glu Phe Leu Ile 
        835                 840                 845             


Pro Val Ser Thr Gly Lys Arg Glu Asn Ala Phe Ile Lys Ser Gln Thr 
    850                 855                 860                 


Gly Lys Leu Phe Ala Lys Leu Val Ala Asp Ser Asn Gly Phe Val Met 
865                 870                 875                 880 


Val Gly Leu Thr Glu Lys Gln His Gly Ala Thr Val Thr Val Gly Lys 
                885                 890                 895     


Lys Val Ser Phe Tyr Asn His Ala Gly Arg Trp Leu Gly Lys Ala His 
            900                 905                 910         


His Ile Ala His Arg Asp Arg Ile Lys Asn Glu Val Asn Gln Val Leu 
        915                 920                 925             


Thr Ser Gly Arg Gly Arg Ile Arg Asn Ile Ala Pro Glu Leu Ser Pro 
    930                 935                 940                 


Lys Thr Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
945                 950                 955         


<210>  97
<211>  941
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.18-NLSںϵ׵İ

<400>  97

Met Thr Asn Gln Lys Pro Lys Phe Lys Ser Ser Asp Ile Gln Ile Lys 
1               5                   10                  15      


His Ile Ser Pro Thr Asp Lys Lys Arg Leu Lys Thr Phe Tyr His Gln 
            20                  25                  30          


Leu Tyr Glu Gln Val Asn Phe Ile Leu Glu Arg Met Ile Val Met Arg 
        35                  40                  45              


Gly Arg Pro Arg Thr Ile Arg Asn Ile Asp Gly Thr Glu Ile Phe Val 
    50                  55                  60                  


Ser Gln Glu Glu Ala Asp Gln Gln Leu Leu Ser Leu Ala Gly Gly Ser 
65                  70                  75                  80  


His Glu Gly Val Lys Tyr Leu Lys Gln Tyr Tyr Glu Ser Cys Val Asp 
                85                  90                  95      


Ala Gly Lys Pro Ala Lys Tyr Ala Ala Asn Met Phe Leu Thr Lys Thr 
            100                 105                 110         


Ile Ser Gly Thr Asn Pro Leu Gln Cys His Thr Ala Val Tyr Lys Leu 
        115                 120                 125             


Tyr Lys Lys Val Gln Ala Lys Gln Ile Thr Lys Lys Glu Phe Ile Asp 
    130                 135                 140                 


Lys Leu Tyr Ser Lys Thr Lys Lys Lys Lys Ser Leu Lys Pro Ala Tyr 
145                 150                 155                 160 


Lys Val Phe Thr Glu Asn Glu His Ile Glu Phe Tyr His Lys Val Arg 
                165                 170                 175     


Ser Gly Lys Leu Pro Ala Ser Glu Val Arg Leu Glu Glu Ser Arg Arg 
            180                 185                 190         


Ala Pro Asp Val Gly Leu Glu Val Gly Leu Leu Leu Arg Glu Leu Gly 
        195                 200                 205             


Ile Phe Pro Phe Asn Phe Pro His Phe Thr Glu Lys Lys Tyr Leu Asp 
    210                 215                 220                 


Leu Ala Trp Thr Ile Ala Ile Arg Trp Leu Lys Asn Trp Asn Glu Asn 
225                 230                 235                 240 


Asn Lys Asn Thr Ala Lys Glu Lys Ala Lys Gln Lys Ala Ile Val Asp 
                245                 250                 255     


Lys Leu Arg Thr Ser Leu Asp Gln Lys Glu Val Asp Leu Phe Glu Glu 
            260                 265                 270         


Phe Ala Glu Glu Cys Ser Gln Glu Gln Phe Gly Ile Arg Glu Gly Phe 
        275                 280                 285             


Val Lys Ala Lys Lys Arg Leu Lys Ser Phe Pro Lys Gly Ile Glu Lys 
    290                 295                 300                 


Ser Ser Tyr Lys Glu Gly Met Arg Ile Leu Val Gln Asn Lys His Gly 
305                 310                 315                 320 


Ser Ile Trp Asp Asn Phe Glu Asn Leu Ala Tyr His His Ile Ala Leu 
                325                 330                 335     


Asn Glu Tyr Asn Arg Leu Arg Asp Glu Ala Ser Phe Ser Phe Pro Asp 
            340                 345                 350         


Pro Ile Tyr His Pro Ile Arg Ala Glu Phe Gly Leu Thr Ser Leu Pro 
        355                 360                 365             


Lys Phe Asn Val Gly Leu Asn Asp Arg Gly Asn Tyr Glu Phe Thr Ile 
    370                 375                 380                 


Asn Leu Pro Asp Gly Pro Leu Met Met Leu Gly Lys Lys Ser Arg Tyr 
385                 390                 395                 400 


Tyr Leu Lys Pro Ile Ile Gln Gly Pro Leu Asn Asn Ala Phe Ser Phe 
                405                 410                 415     


Glu Phe Ile Lys Gly Asn Lys Lys Arg Pro Lys Ile Ser Ala Lys Leu 
            420                 425                 430         


Lys Ser Ile Thr Val Val Phe Ala Lys Ser Ser Ile Tyr Val Gly Leu 
        435                 440                 445             


Pro Tyr Arg Pro Ile Ser Ile Pro Ile Pro Gln Ala Val Thr Asn Ser 
    450                 455                 460                 


Thr Tyr Tyr Phe Lys Lys Asn Leu Ser Ser Thr Ser Lys Phe Asp Lys 
465                 470                 475                 480 


Asp Val Phe Met Gly Leu Thr Ala Val Ser Val Asp Leu Gly Leu Asn 
                485                 490                 495     


Pro Val Phe Ser Met Ser Ala Cys Arg Leu Asp Glu Met Lys Ala Asp 
            500                 505                 510         


Glu His Tyr Ser Cys Glu Val Pro Gly Phe Gly Trp Ala Asn Gln Ile 
        515                 520                 525             


Trp Ser Lys Arg Ala Gly Gly Val Trp Asn Arg Ser Phe Arg Asp Lys 
    530                 535                 540                 


Ile Arg Gly Phe Val Pro Gly Asn Leu Ser Asp Arg Ile Phe Cys Cys 
545                 550                 555                 560 


Lys Lys Ser Ile Ile Val Ser Lys Lys Leu Arg Asp Glu Lys Pro Leu 
                565                 570                 575     


Thr Gln Tyr Glu Glu Glu Asn Phe Glu Arg Trp Met Gln Val Val Gly 
            580                 585                 590         


Val Asp Pro Asn Glu Asp His Tyr Lys Gln Leu Arg Ile Ala Ile Arg 
        595                 600                 605             


Asp Ile Lys Thr Glu Tyr Glu Thr Val Arg Ser Glu Phe Ala Leu Arg 
    610                 615                 620                 


Asp His Pro Asn Asn Ser Asn Lys Thr Thr Glu Asn Ile Cys Thr Glu 
625                 630                 635                 640 


Cys Phe Asp Met Leu Phe Val Ile Lys Asn Leu Ile Ser Leu Leu Lys 
                645                 650                 655     


Ser Trp Asn Arg Trp His Arg Thr Thr Gly Asp Ile Glu Glu Arg Gly 
            660                 665                 670         


Lys Asp Pro Asn Glu Cys Ser Thr Tyr Trp Arg His Tyr Asn Gly Leu 
        675                 680                 685             


Lys Thr Asp Leu Leu Lys Lys Leu Thr Asn Ile Leu Ile Glu Ser Ala 
    690                 695                 700                 


Lys Ser Ile Gly Ala His Ile Ile Ile Leu Glu Asp Leu Thr Leu Ser 
705                 710                 715                 720 


Gln Arg Ser Ser Arg Ser Arg Arg Glu Asn Ser Leu Val Ala Ile Phe 
                725                 730                 735     


Gly Ala Gln Thr Ile Ile Lys Thr Ile Ser Glu Glu Ala Glu Ile Asn 
            740                 745                 750         


Gly Ile Leu Val Tyr Leu Glu Asp Pro Arg His Ser Ser Gln Ile Ser 
        755                 760                 765             


Ile Val Thr Asn Glu Phe Gly Tyr Arg Pro Lys Glu Asp Lys Ala Lys 
    770                 775                 780                 


Leu Tyr Phe Met Asp Glu Glu Thr Val Cys Val Thr Asn Cys Asp Asp 
785                 790                 795                 800 


Ser Ala Ala Leu Met Leu Gln Gln Ser Phe Trp Ser Arg His Lys Asp 
                805                 810                 815     


Val Val Lys Val Lys Gly Thr Lys Val Ser Asp Thr Glu Tyr Leu Val 
            820                 825                 830         


Ser Ser Glu Asp Lys Asp Gly Thr Lys Met Arg Leu Arg Ser Tyr Leu 
        835                 840                 845             


Lys Arg Asn Val Gly Thr Ala Asn Ala Ile Leu Gln Lys Asn Cys Asp 
    850                 855                 860                 


Gly Tyr Asp Leu Lys Lys Ile Ser Pro Gln Lys Lys Lys Lys Ile Glu 
865                 870                 875                 880 


Glu Phe Gly Lys Asp Glu Tyr Phe Tyr Arg His Gly Glu Gln Trp Phe 
                885                 890                 895     


Thr Ala Asp Ala His Phe Asp Lys Leu Arg Glu Phe Gly Asn Gln Val 
            900                 905                 910         


Phe Leu Thr Pro Gln Ser Gln Ile Lys Arg Ile Asn Leu Gln Val Glu 
        915                 920                 925             


Gly Thr Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
    930                 935                 940     


<210>  98
<211>  919
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.19-NLSںϵ׵İ

<400>  98

Met Pro Ser Tyr Lys Ser Ser Arg Val Leu Val Arg Asp Val Pro Glu 
1               5                   10                  15      


Glu Leu Val Asp His Tyr Glu Arg Ser His Arg Val Ala Ala Phe Phe 
            20                  25                  30          


Met Arg Leu Leu Leu Ala Met Arg Arg Glu Pro Tyr Ser Leu Arg Met 
        35                  40                  45              


Arg Asp Gly Thr Glu Arg Glu Val Asp Leu Asp Glu Thr Asp Asp Phe 
    50                  55                  60                  


Leu Arg Ser Ala Gly Cys Glu Glu Pro Asp Ala Val Ser Asp Asp Leu 
65                  70                  75                  80  


Arg Ser Phe Ala Leu Ala Val Leu His Gln Asp Asn Pro Lys Lys Arg 
                85                  90                  95      


Ala Phe Leu Glu Ser Glu Asn Cys Val Ser Ile Leu Cys Leu Glu Lys 
            100                 105                 110         


Ser Ala Ser Gly Thr Arg Tyr Tyr Lys Arg Pro Gly Tyr Gln Leu Leu 
        115                 120                 125             


Lys Lys Ala Ile Glu Glu Glu Trp Gly Trp Asp Lys Phe Glu Ala Ser 
    130                 135                 140                 


Leu Leu Asp Glu Arg Thr Gly Glu Val Ala Glu Lys Phe Ala Ala Leu 
145                 150                 155                 160 


Ser Met Glu Asp Trp Arg Arg Phe Phe Ala Ala Arg Asp Pro Asp Asp 
                165                 170                 175     


Leu Gly Arg Glu Leu Leu Lys Thr Asp Thr Arg Glu Gly Met Ala Ala 
            180                 185                 190         


Ala Leu Arg Leu Arg Glu Arg Gly Val Phe Pro Val Ser Val Pro Glu 
        195                 200                 205             


His Leu Asp Leu Asp Ser Leu Lys Ala Ala Met Ala Ser Ala Ala Glu 
    210                 215                 220                 


Arg Leu Lys Ser Trp Leu Ala Cys Asn Gln Arg Ala Val Asp Glu Lys 
225                 230                 235                 240 


Ser Glu Leu Arg Lys Arg Phe Glu Glu Ala Leu Asp Gly Val Asp Pro 
                245                 250                 255     


Glu Lys Tyr Ala Leu Phe Glu Lys Phe Ala Ala Glu Leu Gln Gln Ala 
            260                 265                 270         


Asp Tyr Asn Val Thr Lys Lys Leu Val Leu Ala Val Ser Ala Lys Phe 
        275                 280                 285             


Pro Ala Thr Glu Pro Ser Glu Phe Lys Arg Gly Val Glu Ile Leu Lys 
    290                 295                 300                 


Glu Asp Gly Tyr Lys Pro Leu Trp Glu Asp Phe Arg Glu Leu Gly Phe 
305                 310                 315                 320 


Val Tyr Leu Ala Glu Arg Lys Trp Glu Arg Arg Arg Gly Gly Ala Ala 
                325                 330                 335     


Val Thr Leu Cys Asp Ala Asp Asp Ser Pro Ile Lys Val Arg Phe Gly 
            340                 345                 350         


Leu Thr Gly Arg Gly Arg Lys Phe Val Leu Ser Ala Ala Gly Ser Arg 
        355                 360                 365             


Phe Leu Ile Thr Val Lys Leu Pro Cys Gly Asp Val Gly Leu Thr Ala 
    370                 375                 380                 


Val Pro Ser Arg Tyr Phe Trp Asn Pro Ser Val Gly Arg Thr Thr Ser 
385                 390                 395                 400 


Asn Ser Phe Arg Ile Glu Phe Thr Lys Arg Thr Thr Glu Asn Arg Arg 
                405                 410                 415     


Tyr Val Gly Glu Val Lys Glu Ile Gly Leu Val Arg Gln Arg Gly Arg 
            420                 425                 430         


Tyr Tyr Phe Phe Ile Asp Tyr Asn Phe Asp Pro Glu Glu Val Ser Asp 
        435                 440                 445             


Glu Thr Lys Val Gly Arg Ala Phe Phe Arg Ala Pro Leu Asn Glu Ser 
    450                 455                 460                 


Arg Pro Lys Pro Lys Asp Lys Leu Thr Val Met Gly Ile Asp Leu Gly 
465                 470                 475                 480 


Ile Asn Pro Ala Phe Ala Phe Ala Val Cys Thr Leu Gly Glu Cys Gln 
                485                 490                 495     


Asp Gly Ile Arg Ser Pro Val Ala Lys Met Glu Asp Val Ser Phe Asp 
            500                 505                 510         


Ser Thr Gly Leu Arg Gly Gly Ile Gly Ser Gln Lys Leu His Arg Glu 
        515                 520                 525             


Met His Asn Leu Ser Asp Arg Cys Phe Tyr Gly Ala Arg Tyr Ile Arg 
    530                 535                 540                 


Leu Ser Lys Lys Leu Arg Asp Arg Gly Ala Leu Asn Asp Ile Glu Ala 
545                 550                 555                 560 


Arg Leu Leu Glu Glu Lys Tyr Ile Pro Gly Phe Arg Ile Val His Ile 
                565                 570                 575     


Glu Asp Ala Asp Glu Arg Arg Arg Thr Val Gly Arg Thr Val Lys Glu 
            580                 585                 590         


Ile Lys Gln Glu Tyr Lys Arg Ile Arg His Gln Phe Tyr Leu Arg Tyr 
        595                 600                 605             


His Thr Ser Lys Arg Asp Arg Thr Glu Leu Ile Ser Ala Glu Tyr Phe 
    610                 615                 620                 


Arg Met Leu Phe Leu Val Lys Asn Leu Arg Asn Leu Leu Lys Ser Trp 
625                 630                 635                 640 


Asn Arg Tyr His Trp Thr Thr Gly Asp Arg Glu Arg Arg Gly Gly Asn 
                645                 650                 655     


Pro Asp Glu Leu Lys Ser Tyr Val Arg Tyr Tyr Asn Asn Leu Arg Met 
            660                 665                 670         


Asp Thr Leu Lys Lys Leu Thr Cys Ala Ile Val Arg Thr Ala Lys Glu 
        675                 680                 685             


His Gly Ala Thr Leu Val Ala Met Glu Asn Ile Gln Arg Val Asp Arg 
    690                 695                 700                 


Asp Asp Glu Val Lys Arg Arg Lys Glu Asn Ser Leu Leu Ser Leu Trp 
705                 710                 715                 720 


Ala Pro Gly Met Val Leu Glu Arg Val Glu Gln Glu Leu Lys Asn Glu 
                725                 730                 735     


Gly Ile Leu Ala Trp Glu Val Asp Pro Arg His Thr Ser Gln Thr Ser 
            740                 745                 750         


Cys Ile Thr Asp Glu Phe Gly Tyr Arg Ser Leu Val Ala Lys Asp Thr 
        755                 760                 765             


Phe Tyr Phe Glu Gln Asp Arg Lys Ile His Arg Ile Asp Ala Asp Val 
    770                 775                 780                 


Asn Ala Ala Ile Asn Ile Ala Arg Arg Phe Leu Thr Arg Tyr Arg Ser 
785                 790                 795                 800 


Leu Thr Gln Leu Trp Ala Ser Leu Leu Asp Asp Gly Arg Tyr Leu Val 
                805                 810                 815     


Asn Val Thr Arg Gln His Glu Arg Ala Tyr Leu Glu Leu Gln Thr Gly 
            820                 825                 830         


Ala Pro Ala Ala Thr Leu Asn Pro Thr Ala Glu Ala Ser Tyr Glu Leu 
        835                 840                 845             


Val Gly Leu Ser Pro Glu Glu Glu Glu Leu Ala Gln Thr Arg Ile Lys 
    850                 855                 860                 


Arg Lys Lys Arg Glu Pro Phe Tyr Arg His Glu Gly Val Trp Leu Thr 
865                 870                 875                 880 


Arg Glu Lys His Arg Glu Gln Val His Glu Leu Arg Asn Gln Val Leu 
                885                 890                 895     


Ala Leu Gly Asn Ala Lys Ile Pro Glu Ile Arg Thr Ser Arg Ala Asp 
            900                 905                 910         


Pro Lys Lys Lys Arg Lys Val 
        915                 


<210>  99
<211>  832
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.20-NLSںϵ׵İ

<400>  99

Met Ala Phe Gln Ser Lys Arg Arg Ile Val Gly Asn Phe Val Lys Glu 
1               5                   10                  15      


Gln Cys Leu Lys Ala Val Asp Gly Lys Val Ile Leu Thr Asp Gln Glu 
            20                  25                  30          


Lys Arg Glu Leu Ile Lys Arg Tyr Glu Leu His Leu Glu Pro His Lys 
        35                  40                  45              


Trp Leu Leu Arg Leu Phe Leu Ser Gly Tyr Glu Gly Arg Asp Asp Gly 
    50                  55                  60                  


Phe Tyr Glu Glu Leu Gly Asn Thr Asn Leu Asp Lys Glu Lys Phe Phe 
65                  70                  75                  80  


Glu Val Thr Ala Gly Leu Arg Asp Ala Leu Leu Arg Gln Ser Gly Ser 
                85                  90                  95      


Ser Arg Ala Leu Lys Ser Ser Met Leu Gly Lys Cys Pro Pro Ser Ala 
            100                 105                 110         


Ala Val Gly Lys Ala Ala Lys His Ile Gln Thr Leu Arg Asp Ala Gly 
        115                 120                 125             


Ile Leu Pro Phe Lys Thr Gly Leu Thr Ser Gly Glu Asp Tyr Asn Val 
    130                 135                 140                 


Leu Gln Gln Ala Val Gln Gln Leu Arg Ser Trp Val Ala Cys Asp His 
145                 150                 155                 160 


Arg Thr Arg Glu Ala Tyr Ala Glu Gln Gln Glu Lys Thr Ser Gln Ala 
                165                 170                 175     


Glu Glu Ala Ala Lys Lys Ala Ala Asn Glu Val Lys Pro Glu Asp Ala 
            180                 185                 190         


Lys Ser Leu Glu Arg His Glu Arg Val Leu Thr Lys Leu Arg Lys Gln 
        195                 200                 205             


Glu Arg Arg Leu Glu Arg Met Lys Ser His Ala Gln Phe Ser Leu Asp 
    210                 215                 220                 


Glu Met Asp Cys Thr Gly Tyr Ser Leu Cys Met Gly Ala Asn Tyr Leu 
225                 230                 235                 240 


Lys Asp Tyr Cys Leu Glu Lys Glu Gly Arg Gly Leu Arg Leu Thr Leu 
                245                 250                 255     


Lys Asn Ser Thr Met Ala Gly Ser Tyr Tyr Val Ser Val Gly Asp Gly 
            260                 265                 270         


Gln His Ala Gly Met Lys Asn Pro Gly Thr Pro Ala Gly Gly Ser Pro 
        275                 280                 285             


Glu Lys Gly Arg Arg Arg Asn Ile Leu Phe Asp Phe Thr Val Glu Lys 
    290                 295                 300                 


Cys Gly Asp Asn Tyr Leu Phe Arg Tyr Asp Glu Asn Gly Lys Arg Pro 
305                 310                 315                 320 


Arg Ala Gly Val Val Lys Glu Pro Arg Phe Cys Trp Arg Arg Lys Gly 
                325                 330                 335     


Asn Ser Val Glu Leu Tyr Leu Ala Met Pro Ile Asn Ile Glu Asn Ser 
            340                 345                 350         


Met Arg Asn Ile Phe Val Gly Lys Gln Lys Ser Gly Lys His Ser Ala 
        355                 360                 365             


Phe Thr Arg Gln Trp Pro Lys Glu Val Glu Gly Leu Asp Glu Leu Arg 
    370                 375                 380                 


Asp Ala Val Val Leu Gly Val Asp Ile Gly Ile Asn Arg Ala Ala Phe 
385                 390                 395                 400 


Cys Ala Ala Leu Lys Thr Ser Arg Phe Glu Asn Gly Leu Pro Ala Asp 
                405                 410                 415     


Val Gln Val Met Asp Thr Thr Cys Asp Ala Leu Thr Glu Lys Gly Gln 
            420                 425                 430         


Glu Tyr Arg Gln Leu Arg Lys Asp Ala Thr Cys Leu Ala Trp Leu Ile 
        435                 440                 445             


Arg Thr Thr Arg Arg Phe Lys Ala Asp Pro Gly Asn Lys His Asn Gln 
    450                 455                 460                 


Ile Lys Glu Lys Asp Val Glu Arg Phe Asp Ser Ala Asp Gly Ala Tyr 
465                 470                 475                 480 


Arg Arg Tyr Met Asp Ala Ile Ala Glu Met Pro Ser Asp Pro Leu Gln 
                485                 490                 495     


Val Trp Glu Ala Ala Arg Ile Thr Gly Tyr Gly Glu Trp Ala Lys Glu 
            500                 505                 510         


Ile Phe Ala Arg Phe Asn His Tyr Lys His Glu His Ala Cys Cys Ala 
        515                 520                 525             


Val Ser Leu Ser Leu Ser Asp Arg Leu Val Trp Cys Arg Leu Ile Asp 
    530                 535                 540                 


Arg Ile Leu Ser Leu Lys Lys Cys Leu His Phe Gly Gly Tyr Glu Ser 
545                 550                 555                 560 


Lys His Arg Lys Gly Phe Cys Lys Ser Leu Tyr Arg Leu Arg His Asn 
                565                 570                 575     


Ala Arg Asn Asp Val Arg Lys Lys Leu Ala Arg Phe Ile Val Asp Ala 
            580                 585                 590         


Ala Val Asp Ala Gly Ala Ser Val Ile Ala Met Glu Lys Leu Pro Ser 
        595                 600                 605             


Ser Gly Gly Lys Gln Ser Lys Asp Asp Asn Arg Ile Trp Asp Leu Met 
    610                 615                 620                 


Ala Pro Asn Thr Leu Ala Thr Thr Val Cys Leu Met Ala Lys Val Glu 
625                 630                 635                 640 


Gly Ile Gly Phe Val Gln Val Asp Pro Glu Phe Thr Ser Gln Trp Val 
                645                 650                 655     


Phe Glu Gln Arg Val Ile Gly Asp Arg Glu Gly Arg Ile Val Ser Cys 
            660                 665                 670         


Leu Asp Ala Glu Gly Val Arg Arg Asp Tyr Asp Ala Asp Glu Asn Ala 
        675                 680                 685             


Ala Lys Asn Ile Ala Trp Leu Ala Leu Thr Arg Glu Ala Glu Pro Phe 
    690                 695                 700                 


Cys Met Ala Phe Glu Lys Arg Asn Gly Val Val Glu Pro Lys Gly Leu 
705                 710                 715                 720 


Arg Phe Asp Ile Pro Glu Glu Pro Thr Arg Glu Gln Asp Glu Ser Asp 
                725                 730                 735     


Gln Asp Phe Lys Lys Arg Leu Glu Glu Arg Asp Lys Leu Ile Glu Arg 
            740                 745                 750         


Leu Gln Ala Lys Ala Asp Arg Met Gln Ala Ile Val Gln Arg Leu Phe 
        755                 760                 765             


Gly Asp Arg Arg Pro Trp Asp Ala Phe Ala Asp Arg Ile Pro Glu Gly 
    770                 775                 780                 


Lys Ser Lys Arg Leu Phe Arg His Arg Asp Gly Leu Val Leu Asn Lys 
785                 790                 795                 800 


Pro Phe Lys Gly Leu Cys Gly Ser Glu Asn Ser Glu Gln Lys Ala Ser 
                805                 810                 815     


Ala Arg Asn Ser Arg Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
            820                 825                 830         


<210>  100
<211>  848
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.21-NLSںϵ׵İ

<400>  100

Met Gly Arg Phe Gly Lys Lys Lys Ile Ala Val Asn Gly Tyr Val Glu 
1               5                   10                  15      


Gln Asp Cys Ile Lys Thr Ile Ser Ala Lys Cys Leu Leu Thr Arg Ala 
            20                  25                  30          


Gln Ile Asp Glu Leu Arg Ala Lys Tyr Asp Ala Val Leu Asp Thr Met 
        35                  40                  45              


Arg Pro Leu Ile Arg Leu Ile Leu Ala Gly Tyr Glu Gly Arg Asp Asp 
    50                  55                  60                  


Gly Ile Tyr Glu Glu Ile Ala Pro Glu Met Ser Lys Lys Lys Phe Phe 
65                  70                  75                  80  


Glu Ala Ala Thr Glu Trp Arg Glu Ser Ile Val Lys Asn Ala Ser Pro 
                85                  90                  95      


Arg Ala Met Lys Ala Ser Val Phe Gly Asp Lys Glu Pro Cys Lys Ser 
            100                 105                 110         


Thr Gly Gly Ala Arg Ala Val Ile Gly Lys Leu Arg Lys Ser Gly Val 
        115                 120                 125             


Phe Pro Ile Glu Thr Gly Leu Ser Gly Gly Asp Glu Tyr Asn Leu Ile 
    130                 135                 140                 


Glu Gln Ala Ile Glu Tyr Ala Lys Ser Trp Leu Lys Ser Asp Glu Ala 
145                 150                 155                 160 


Thr Arg Glu Ala Tyr Ala Asp Gln Gln Lys Asp Ile Lys Arg Leu Ile 
                165                 170                 175     


Gly Glu Ala Lys Lys Leu Ala Leu Lys Ile Glu Lys Ala Glu Lys Lys 
            180                 185                 190         


Leu Glu Ala Thr Asn Pro Gln Thr Lys Ser Trp Lys Lys Thr Thr Glu 
        195                 200                 205             


Ile Ile Lys Lys Ser Lys Arg Glu Phe Gly Ser Val Thr Thr Lys Thr 
    210                 215                 220                 


Glu Lys Ala Glu Lys Arg Phe Glu Arg Met Lys Pro Phe Ser Lys Leu 
225                 230                 235                 240 


Glu Leu Gln Asn Met Asp Cys Thr Lys Tyr Ser Thr Tyr Leu Gly Thr 
                245                 250                 255     


Asn Tyr Ser Pro Phe Lys Leu Lys Lys Glu Gly Asp Leu Leu Gln Ile 
            260                 265                 270         


Thr Val Thr Ser Ser Val Met Lys Gly Thr Tyr Leu Ala Ser Tyr Gly 
        275                 280                 285             


Asp Gly Gln Tyr Gly Ser Arg Arg Asn Asn Gly Gln Ser Arg Arg Asp 
    290                 295                 300                 


Asp Phe Val Pro Asn Met Asn Gln Lys Arg Arg Arg Asn Leu Met Phe 
305                 310                 315                 320 


Asp Cys Thr Val Glu Pro Phe Gly Asp Gly Ser Leu Leu Arg Tyr Glu 
                325                 330                 335     


Glu Asn Gly Leu Arg Pro Arg Val Ala Glu Leu Lys Glu Pro Arg Leu 
            340                 345                 350         


Cys Trp Arg Arg Arg Asn Gly Asn Tyr Glu Leu Tyr Leu Met Met Pro 
        355                 360                 365             


Val Lys Met His Val Lys Ser Pro Glu Met Phe Ala Gly Asp His Leu 
    370                 375                 380                 


Ala Phe Ser Arg Tyr Trp Pro Lys Glu Val Glu Gly Leu Asp Ser Asp 
385                 390                 395                 400 


Thr Lys Ile Thr Ala Leu Gly Val Asp Val Gly Ile Ile Arg Ser Ala 
                405                 410                 415     


Tyr Cys Val Ala Val Thr Ala Glu Arg Phe Val Asp Gly Leu Pro Thr 
            420                 425                 430         


Glu Met Thr Val Gly Lys Ala Ser Phe Asp Ala Gln Thr Glu Lys Gly 
        435                 440                 445             


Arg Glu Tyr Phe Glu Leu Gly Arg Arg Ala Thr Met Leu Gly Trp Leu 
    450                 455                 460                 


Ile Lys Thr Thr Arg Arg Tyr Lys Lys Asp Pro Lys Asn Glu His Asn 
465                 470                 475                 480 


Gln Ile Lys Glu Ser Asp Val Ala Ala Phe Asp Gly Ser Pro Gly Ala 
                485                 490                 495     


Phe Glu His Tyr Ile Leu Ala Val Asp Glu Met Ser Asp Asp Pro Leu 
            500                 505                 510         


Asp Val Trp Gly His Ala Asn Ile Thr Gly Tyr Gly Lys Trp Thr Lys 
        515                 520                 525             


Gln Ile Phe Lys Glu Phe Asn Gln Leu Lys Arg Glu Arg Ala Glu Gly 
    530                 535                 540                 


Gln Val Glu Pro Asn Met Thr Asp Asp Leu Thr Trp Cys Ser Leu Ile 
545                 550                 555                 560 


Asp Tyr Ile Ile Ser Leu Lys Lys Thr Leu His Phe Gly Gly Tyr Glu 
                565                 570                 575     


Thr Lys Glu Arg Glu Ser Phe Cys Pro Ala Leu Tyr Asn Glu Arg Ala 
            580                 585                 590         


Asn Cys Arg Asp Val Val Arg Lys Arg Leu Ala Arg Tyr Val Val Glu 
        595                 600                 605             


Arg Ala Ile Ala Ala Glu Ala Gln Val Ile Ser Val Glu Asn Leu Ser 
    610                 615                 620                 


Lys Cys Arg Arg Asp Asp Lys Arg Lys Asn Arg Val Trp Asp Leu Met 
625                 630                 635                 640 


Ser Gln Gln Ser Trp Ile Gly Val Leu Thr Asn Met Ala Arg Met Glu 
                645                 650                 655     


Asn Ile Ala Val Val Ser Val Asn Pro Asp Leu Thr Ser Gln Trp Val 
            660                 665                 670         


Glu Gln Cys Gly Ala Ile Gly Asp Arg Lys Ala Arg Thr Ile Ala Cys 
        675                 680                 685             


Arg Asp Val Asn Gly Lys Phe Val Ser Leu Asp Ala Asp Leu Asn Ala 
    690                 695                 700                 


Ala Tyr Asn Ile Ala Ser Arg Ala Leu Thr Arg His Ala Glu Pro Phe 
705                 710                 715                 720 


Ser Ile Thr Phe Lys Lys Lys Asp Gly Ile Leu Glu Gln Lys Asp Val 
                725                 730                 735     


Cys Phe Asp Pro Gly Val Ile Pro Val Leu Glu Lys Asn Glu Asn Glu 
            740                 745                 750         


Glu Lys Phe Arg Glu Arg Val Glu Lys Tyr Glu Lys Ser Leu Val Ile 
        755                 760                 765             


Lys Gln Glu Arg Ala Val Arg Trp Arg Ala Ile Leu Gln His Leu Phe 
    770                 775                 780                 


Gly Asn Glu Arg Pro Trp Asp Glu Phe Thr Asp Glu Val Lys Glu Gly 
785                 790                 795                 800 


Arg His Val Ser Leu Tyr Arg His His Gly Lys Leu Val Arg Thr Lys 
                805                 810                 815     


Gln Tyr Ala Gly Leu Val Lys Glu Ala Asn Asn Glu Leu Val Pro Val 
            820                 825                 830         


Cys Ala Val Ala Arg Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
        835                 840                 845             


<210>  101
<211>  979
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.22-NLSںϵ׵İ

<400>  101

Met Ser Lys Ala Thr Arg Lys Thr Lys Thr Thr Val Pro Glu Ser Thr 
1               5                   10                  15      


Asp Thr Glu Ser Pro Ala Ala Asp Thr Gln Val Arg Val His Trp Leu 
            20                  25                  30          


Ala Ala Ser His Arg Ala Ser Pro Gly Leu Gln Gln Val Lys Glu Met 
        35                  40                  45              


Ile Gln Gln His Ala Asp Val Ala Ser Val Leu Phe Gln Gly Leu Val 
    50                  55                  60                  


Arg Thr Ala Pro Ile Val Phe Arg Asn Asp Asp Gly Ser Pro Val Lys 
65                  70                  75                  80  


Pro Leu Asp Leu Leu Leu Ala Ser Leu Arg Pro Thr Tyr Lys Val Gln 
                85                  90                  95      


Arg Asp Thr Glu Thr Val Leu Val Thr Lys Asp Asp Val Ile Arg Cys 
            100                 105                 110         


Leu Thr Leu Ala Thr Thr Ala Val Asn Gly Gly Gln Ala Thr Asn Val 
        115                 120                 125             


Ala Val Phe Ala Ser Ala Asp Pro Ala Leu Ser Ala Pro Leu Ala Thr 
    130                 135                 140                 


Leu Leu Ala Gln Leu Arg Ala Leu Glu Ser Val Asp Ser Ser Trp Ser 
145                 150                 155                 160 


Val Val Gly Lys Leu Asp Ile Asn Leu Arg Lys Phe Val Trp Leu Val 
                165                 170                 175     


Leu Ser Ala Ala Gly Val Leu Pro Ala Leu Ala Asp Leu Glu Gly Tyr 
            180                 185                 190         


Ala Ala Lys Ser Val Leu Ala Asn Val Gln Gly Lys Tyr Lys Ser Leu 
        195                 200                 205             


Gln Ala Cys Ala Asp Thr His Ala Ala Leu Tyr Lys Gln His Gln Thr 
    210                 215                 220                 


Asn Lys Glu Gln Leu Glu Lys Leu Ile Ala Asp Pro Gly Phe Val Ala 
225                 230                 235                 240 


Leu Cys Ser Ala Leu Leu Gln Asp Pro Asp Leu Arg Ser Val Asp Ser 
                245                 250                 255     


Arg Arg Leu Ala Ala Leu Glu Glu Met Leu Gly Phe Val Ala Ala Asp 
            260                 265                 270         


Lys Asn Tyr Ser Glu Tyr Thr Ser Thr Arg Lys Cys Asp Gly Trp Ala 
        275                 280                 285             


Pro Pro Ala Asn Met Phe Asp Leu Leu Cys Glu His Lys Glu Ala Val 
    290                 295                 300                 


Arg Arg Asn Ile Val Val Asp Asn Ser Lys Cys Leu Ser Arg Arg Ile 
305                 310                 315                 320 


Ser Leu Val Ala Asp Gly Asp Val Asn Glu Val Ser Val Phe Glu Leu 
                325                 330                 335     


Leu Asn Glu Met Arg Trp Leu Ser Val His Ser Ser Gly Ile Arg Met 
            340                 345                 350         


Pro Asn Tyr Pro Lys His Ala Tyr Ala Leu Lys Phe Gly Asp Asn Tyr 
        355                 360                 365             


Ile Ser Val Lys Ser Phe Glu Thr Val Val Asp Gly Gly Cys Ser Leu 
    370                 375                 380                 


Leu Arg Met Thr Ala Arg Val Gly Lys Asn Asp Leu Val Cys Asp Phe 
385                 390                 395                 400 


Val Leu Gly Arg Gly Asn Glu Tyr Trp Asn Asn Leu Lys Ile Thr Pro 
                405                 410                 415     


Met Gly Lys Gly Ile Phe Ala Val Val Lys Thr Val Arg Arg Phe Thr 
            420                 425                 430         


Ala Thr Gly Ala Lys Leu Val Glu Leu Arg Gly Val Cys Lys Glu Pro 
        435                 440                 445             


Glu Ile Arg Tyr Glu Arg Gly Val Leu Gly Leu Arg Leu Pro Ile Ser 
    450                 455                 460                 


Phe Asp Val Tyr Gly Lys Val Glu Glu Asp Ser Ile Ala Phe Gly Lys 
465                 470                 475                 480 


Asn Arg Val Ser Leu Arg Thr Thr Pro Phe Val Glu Lys Ala Asp Lys 
                485                 490                 495     


Phe Gln Gly Leu Leu Asp Tyr Arg Asn Thr Thr Ala Arg Asp Gly Tyr 
            500                 505                 510         


Ile Tyr Tyr Ala Gly Phe Asp Gln Gly Glu Asn Asp Gln Val Val Gly 
        515                 520                 525             


Ile Tyr Arg Thr Arg Thr Tyr Lys Asn Ala Thr Met Leu Glu Phe Phe 
    530                 535                 540                 


Asn Val Ser Asp Thr Leu Glu Glu Val Ala Ser Cys Arg Phe Ser Asp 
545                 550                 555                 560 


Tyr Gln Glu Arg Lys Arg Arg Leu Arg Gly Asp Thr Gly Val Leu Asp 
                565                 570                 575     


Ile Asn Ser Ile Asn Val Leu Ala Asp Lys Val Gln Arg Leu Arg Arg 
            580                 585                 590         


Leu Ile Ser Thr Leu Arg Ala Cys Ala Ser His Thr Asp Trp Tyr Pro 
        595                 600                 605             


Lys Leu Lys Glu Arg Arg Arg Leu Glu Trp Ala Val Leu Ala Gln Gly 
    610                 615                 620                 


Val Gly Val Ser Asp Phe Asp Thr Glu Ile Glu Arg Ala Glu Thr Ala 
625                 630                 635                 640 


Leu Ser Ala Val Ala Ala Val Asp Phe Val Arg Asp Pro Thr Cys Ile 
                645                 650                 655     


Ile Asn Val Met Asp Lys His Ile Tyr Ala Gln Phe Lys Gln Leu Arg 
            660                 665                 670         


Ser Glu Arg Asn Glu Lys Tyr Arg Ser Gln His Gln His Asp Tyr Lys 
        675                 680                 685             


Trp Leu Gln Leu Val Asp Ser Val Ile Ser Leu Arg Lys Ser Ile Tyr 
    690                 695                 700                 


Arg Phe Gly Lys Ala Pro Glu Pro Arg Gly Ala Gly Glu Leu Tyr Pro 
705                 710                 715                 720 


Gln Asn Leu Tyr Thr Tyr Arg Asp Asn Leu Met Gln Gln Tyr Arg Lys 
                725                 730                 735     


Glu Val Ala Ala Phe Ile Arg Asp Val Cys Leu Glu His Gly Val Arg 
            740                 745                 750         


Gln Leu Ala Val Glu Ala Leu Asn Pro Thr Ser Tyr Ile Gly Glu Asp 
        755                 760                 765             


Ser Asp Ala Asn Arg Lys Arg Ala Leu Phe Ala Pro Ser Glu Leu His 
    770                 775                 780                 


Asn Asp Ile Val Leu Ala Cys Ser Leu His Ser Ile Ala Val Val Ala 
785                 790                 795                 800 


Val Asp Glu Thr Met Thr Ser Arg Val Ala Pro Asn Asn Arg Leu Gly 
                805                 810                 815     


Phe Arg Ser His Gly Asp Tyr Gln Lys Phe Ser Glu Thr Ala Gln Gly 
            820                 825                 830         


Arg Phe Asn Trp Lys His Leu His Tyr Phe Gly Asp Asn Asp Val Ser 
        835                 840                 845             


Glu His Cys Asp Ala Asp Glu Asn Ala Cys Arg Asn Ile Val Leu Arg 
    850                 855                 860                 


Ala Leu Thr Cys Gly Ala Ser Lys Pro Arg Phe Ser Arg Gln Ser Leu 
865                 870                 875                 880 


Leu Gly Lys Ile Lys Gly Pro Val Leu Arg Thr Gln Leu Ala Tyr Leu 
                885                 890                 895     


Ala His Lys Arg Gly Leu Leu Thr Ala Ser Thr Glu Pro Lys Lys Ala 
            900                 905                 910         


Ala Glu Thr Gly Phe Glu Leu Val Glu Ala Asp Leu Gly Gly Ala Leu 
        915                 920                 925             


Arg Val Gly Lys Gly Phe Ile Tyr Val Asp Ala Gly Ile Cys Ile Asn 
    930                 935                 940                 


Ala Thr Thr Arg Lys Glu Arg Ser His Lys Val Gly Glu Ala Val Val 
945                 950                 955                 960 


Ser Arg Ser Leu Ala Ser Pro Phe Ser Arg Ala Asp Pro Lys Lys Lys 
                965                 970                 975     


Arg Lys Val 
            


<210>  102
<211>  3268
<212>  DNA
<213>  ˹

<220>
<223>  Cas12j.3ϵͳ

<400>  102
tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatgacc aaggagaaga       60

tcaagaagac caagaaggcc aaggtggaga aggactccgt gaccagggcc ggcatcctga      120

ggatcctgct gaacccggac cagcaccagg agctggacac cctgatctcc gaccaccagg      180

aggccgccag ggagatccag accgccacct acaagctgtc cggcctgaag ctgtacgaca      240

agaccaacaa catggtggtg gacggctcca aggccacccc ggaggagcag gaggcctact      300

acaagatcat caactgggag ggccagccga tctccatctc caacccgatg gtgagggcca      360

ccttcaagtc catcgccaag gtgaaggagg acatcaggag gaagcaggag gagtacgcca      420

agctggagga ggccgacctg accaagatgt ccaccggcga cgtgaagaag cacaagaacg      480

agctgaggaa ggccgccaac aggatcaagc actccgagga gatcctgcag ttcgccaagt      540

ggaggctggc cgacatcttc ccgctgccgc tgtcccacaa ctcccagctg cacctgaaga      600

acaactacca ccagaacgtg ttctccggct tccacgccag ggtgaagggc tggaacgcct      660

gcgacatcgc cgcccaggcc aactacgccg agatcgacaa caggctgacc gagctgtcct      720

ccgagctgtc cggcgactac ggctccgagg tgatcaccga cctgatgggc ctgctgcagt      780

acaccaagga gctgggcgag ggctacaccg acacctccta cctgaactac aagttcctgt      840

ccttcttcaa ggagtgctgg aggccgaacg ccatcgccaa caacaccggc ctgctggagg      900

gcttctggct ggccaacaac aagcacacca acaagaagaa ccaggtggcc tactccttca      960

acccgaagat ctccgaggag ctgttcagga ggaggtccct gtgggagtcc gacaagtgcc     1020

tgctgtccga cccgaggttc gagaagtacg tggagctgtt cgacaagcac ggcaggtaca     1080

ggaagggcgc ctccctgacc ctgatctcca aggagtcccc gatcccgatc ggcttctcca     1140

tggacaggaa cgccgccaag ctggtgagga tcgacaacga caccgccaac aggcagctga     1200

ccatcaccat cgagctgccg aacaaggagg agaggtccta cgtggccgcc tacggcagga     1260

agcacgagac caagtgctac tacaacggcc tgaccaccag gctgccgagg tccgagaagg     1320

agctgctggc cctggccaag gccgagaaca gggagctgac cgacaaggag atccacgagg     1380

cctccctgga gaagtgctac atcttcgagt acgccagggc cggcaagatc ccggtgttcg     1440

ccgtggtgaa gaccctgtac ttcaggagga acccgtccaa cggcgagtac tacgtgatcc     1500

tgccgaccaa catcttcgtg gagtaccacg ccaacaacga gttcaactcc aaggagctgt     1560

tcaagatcag gtccgagctg cagaaggcct gggacgaggt gaggaccccg aagaggaacg     1620

tgcagtcctg cgtgctggac aaggacctgt ccaagaggtt cgccggcagg accctgaagt     1680

acgccggcat cgacctgggc tactccaacc cgtacaccgt gtcctactac aacgtggtgg     1740

gcaccgagga gggcatccag atcaaggaga ccggcaacga gatcgtgtcc accgtgttca     1800

acgagcagta catccagctg aagggcaaca tctaccagct gatcaacatc atcagggcct     1860

ccaggaggta cctgcaggag tccggcgagc tgaagctgtc caaggacgac atcaagtcct     1920

tcgaccagct gatggagctg ctgccgtccg agcagaggat caccatcgac cagttcatca     1980

aggacatcaa gaaggccaag caggagggca agctgatcag ggacatcaag ggcaagctgc     2040

cggtggaggg caagaagaag gagtactggg tgatctccaa cctgatgtac gtgatcaccc     2100

agaccatgaa cggcatcagg ggcaacaggg actccaacaa ccacctgacc gagaagaaga     2160

actggctgtc cgccccgccg ctgatcgagc tgatcgacgc ctactacaac ctgaagaaga     2220

ccttcaacga ctccggcgac ggcatcaaga tgctgccgaa ggaccacgtg tacgccgagg     2280

gcgagaagca gaggtgcacc ctgagggagg agaacttctg caagggcatc ctggagtgga     2340

gggacaacgt gaaggactac ttcatcaaga agctgttctc ccagatcgcc cacaggtgct     2400

acgagctggg catcggcatc gtggccatgg agaacctgga catcatgggc tcctccaaga     2460

acaccaagca gtccaacagg atgttcaaca tctggccgag gggccagatg aagaagtccg     2520

ccgaggacgc cttctcctac atgggcatcc tgatccagta cgtggacgag aacggcacct     2580

ccaggcacga cgccgactcc ggcatctacg gctgcaggga cggcgccaac ctgtggctgc     2640

cgaacaagaa gctgcacgcc gacgtgaacg cctccaggat gatcgccctg aggggcctga     2700

cccaccacac caacctgtac tgcaggtccc tgaccgagat cgagaacggc aagtacgtga     2760

acacctacga gctgttcgac accaccaaga acgaccagtc cggcgccgcc aagaggctga     2820

ggggcgccga gaccctgctg cacggctact ccgccaccgt gtaccagatc cacaccacca     2880

acaccggcgc cggcgtggcc ctgctgccgg acctgaccgc caccgacgtg atcaagaaca     2940

agaagatcac cgccaccaag gagaacaccg ccaagtacta caagctggac aacaccaaca     3000

cctactaccc gtggtccgtg tgcgagaagc tgcacaagaa ctggaagctg tcctgacaaa     3060

taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga     3120

acgctctcct gagtaggaca aatttgacag ctagctcagt cctaggtata atgctagcgg     3180

tgatatagta actggtctgt tccagcactt caccggtata acaacttcga cgagctctac     3240

aagaaggcca tcctgacgga tggccttt                                        3268


<210>  103
<211>  35
<212>  DNA
<213>  ˹

<220>
<223>  PAMĿ


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  n = a or t or c or g

<400>  103
nnnnnnnngg tataacaact tcgacgagct ctaca                                  35


<210>  104
<211>  27
<212>  RNA
<213>  ˹

<220>
<223>  Pre-crRNAӹPAMĵRNA

<400>  104
gguauaacaa cuucgacgag cucuaca                                           27


<210>  105
<211>  24
<212>  RNA
<213>  ˹

<220>
<223>  Cas12j.19RNA

<400>  105
cuuccaucag agaaccucac ugcg                                              24


<210>  106
<211>  1020
<212>  DNA
<213>  ˹

<220>
<223>  ˫DNA

<400>  106
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accccctctt      420

cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc      480

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtacca      540

tcgtattagg tatagcaagc cgtctcgcag tgaggttctc tgatggaagc atatcgtagc      600

ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca      660

cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa      720

ctggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc      780

tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca      840

taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct      900

cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac      960

gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc     1020


<210>  107
<211>  882
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.1

<400>  107

Met Leu Tyr Thr Met Asn Val Lys Thr Ile Lys Leu Lys Val Asp Ala 
1               5                   10                  15      


Thr Lys Glu Val Glu Ser Arg Leu Thr Lys Met Leu Leu Val His Asn 
            20                  25                  30          


Asn Ile Gly Arg Glu Ile Ile Asn Phe Leu Ile Leu Cys Ser Gly Asn 
        35                  40                  45              


Asp Asn Ile Arg Lys Thr Lys Phe Asp Glu Phe Gly Asn Ser Tyr Asp 
    50                  55                  60                  


Glu Phe Cys Asn Leu Lys Leu Asp Gln Phe Asn Leu Tyr Asp Arg Leu 
65                  70                  75                  80  


Thr Glu Ile His Asp Glu Val Thr Leu Glu Asp Phe Gln Lys Thr Leu 
                85                  90                  95      


Asn Asp Ile Tyr Asp Leu Val Leu Asn Ser Lys Ser Phe Ser Asn Val 
            100                 105                 110         


Ser Ser Thr Ile Phe Asn Lys Asn Lys Lys Val Asn Phe Asp Glu Thr 
        115                 120                 125             


Lys Lys Gly Asp Leu Ser Arg Lys Cys Leu Met Asn Ala Arg Asp Trp 
    130                 135                 140                 


Gly Val Leu Pro Leu Ile Ser Val Asp Asp Asp Ile Val Thr Cys Gly 
145                 150                 155                 160 


Thr Leu Lys Gly Ile Leu Ser Glu Cys Gln Ser Arg Ile Leu Ser Trp 
                165                 170                 175     


Asn Glu Cys Asn Leu Ser Thr Lys Glu Thr Tyr Ser Glu Lys Lys Ser 
            180                 185                 190         


Glu Tyr Gln Ser Ile Leu Asp Asp Ser Met Thr Lys Asp Ala Asp Val 
        195                 200                 205             


Thr Thr Ala Met Ile Gln Phe Met Asp Asp Val Ser Asn Val Tyr Gly 
    210                 215                 220                 


Ser Asn Asn Glu Asn Gln Leu Lys Trp Phe Asn Asn Arg Phe Leu Thr 
225                 230                 235                 240 


Tyr Val Arg Asn Lys Ile Arg Pro Phe Leu Leu Thr Asn Ser Pro Ile 
                245                 250                 255     


Asp Asn Phe Glu Gln Ser Asp Thr Ser Tyr Asn Cys Ser Ile Glu Ile 
            260                 265                 270         


Val Arg Ile Leu Ser Lys Tyr Glu Ile Leu Trp Lys Asp Glu Val Ser 
        275                 280                 285             


Val Asn Arg Tyr Lys Lys Thr Cys Asp Asp Gly Ile Asn Ile Glu Lys 
    290                 295                 300                 


Tyr Arg Tyr Leu Val His Ala Lys Ser Asp Phe Leu Arg Tyr Lys Glu 
305                 310                 315                 320 


Thr Ala Ser Phe Lys Glu Ile His Ala Val Lys Ser Pro Ile Ser Leu 
                325                 330                 335     


Cys Phe Gly Asn Asn Tyr Gln Pro Phe Ser Leu Ser Asp Val Gly Asp 
            340                 345                 350         


Arg His Asn Ile Asn Phe Gly Tyr Lys Phe Gly Lys Leu Gly Lys Gln 
        355                 360                 365             


Arg Lys Glu Cys Ser Phe Asn Leu Asn Tyr Arg Arg Lys Lys Val Lys 
    370                 375                 380                 


Tyr Ala Asn Thr Pro Val Arg Ser Asp Glu Asn Lys Cys Tyr Leu Asp 
385                 390                 395                 400 


Asn Leu Glu Ile Glu Asp Ala Lys Asn Gly Ser Tyr Lys Leu Ser Tyr 
                405                 410                 415     


Met Val Asn Lys Lys Tyr Lys Arg Glu Ser Phe Ile Lys Glu Pro Lys 
            420                 425                 430         


Met Lys Met Tyr Asn Gly Lys Leu Tyr Met Tyr Phe Pro Met Ser Asn 
        435                 440                 445             


Glu Phe Glu Glu Asp Arg Asp Ser Phe Ala Leu Leu Thr Tyr Phe Ser 
    450                 455                 460                 


Arg Ser Ser Asn Ser Lys Ser Gln Ile Asp Glu Ala Ser Asn Ile Leu 
465                 470                 475                 480 


Gln Asn Arg Lys Ile Arg Val Cys Gly Val Asp Leu Gly Ile Asn Pro 
                485                 490                 495     


Thr Phe Ala Leu Ser Val Leu Glu Tyr Ser Asp Asn Lys Ile Thr Asp 
            500                 505                 510         


Thr Asn Ile Gly Met Lys His Glu Gly Ser Tyr Asn Asn Phe Ser Glu 
        515                 520                 525             


Ile Arg Lys Gln Ile Asn Asp Val Thr Asp Met Ile Ser Tyr Leu Lys 
    530                 535                 540                 


Ser Lys Tyr Asp Asn Cys Glu Lys Asp Tyr Ser Ser Lys Ile Asp Asp 
545                 550                 555                 560 


His Ile Lys Ser Arg Leu Asn Glu Glu Ile Ser Asn Phe Cys Asp Leu 
                565                 570                 575     


Val Ser Tyr Lys Arg Asn Lys Asn Thr Ile Ile Arg Lys Glu Ile Lys 
            580                 585                 590         


Asn Val Glu Lys Glu Ile Asn Lys Ile Lys Asn Cys Arg Arg His Thr 
        595                 600                 605             


Leu Lys Lys Asp Leu Thr Glu Asn Phe Gly Trp Val Ser Ala Leu Asn 
    610                 615                 620                 


Glu Phe Ile Ser Leu Lys His Ser Phe Asn Asp Met Gly Glu Ser Phe 
625                 630                 635                 640 


Asp Ser Lys Thr Asn Pro Ser Tyr Ser Tyr Phe Glu Lys Trp Lys Arg 
                645                 650                 655     


Tyr Ile Asp Asn Ile Lys Asp Asp Ser Leu Lys Thr Val Ser Arg Glu 
            660                 665                 670         


Ile Leu Asn Phe Cys Ile Glu Asn Ser Val Asp Phe Ile Ala Leu Glu 
        675                 680                 685             


Asp Leu Gln Thr Phe Ala Pro Ser Asp Asp Arg Thr Lys Ser His Asn 
    690                 695                 700                 


Lys Leu Thr Gln Leu Trp Cys Phe Gly Lys Leu Lys Lys Cys Leu Glu 
705                 710                 715                 720 


Asp Ile Ala Ser Met Tyr Gly Ile His Val Tyr Ser Ser Thr Asp Pro 
                725                 730                 735     


Arg Asn Thr Ser Asp Thr His Phe Glu Ser Lys Asn Phe Gly Tyr Arg 
            740                 745                 750         


Asp Glu Ser Asn Lys His Asn Leu Trp Val Asn Val Asp Gly Glu Tyr 
        755                 760                 765             


Thr Val Val Asp Ser Asp Ile Asn Ala Ser Lys Asn Ile Ala Asn Arg 
    770                 775                 780                 


Phe Leu Thr His His Lys Asp Leu Lys Gln Leu Pro Met Ile Gly Asp 
785                 790                 795                 800 


Gly Thr Leu Phe Lys Ile Asp Ser Ser Ser Lys Arg Asn Lys Ser Phe 
                805                 810                 815     


Ala Val Lys Leu Asn Ile His Lys Asn Val Tyr Glu Leu Ile Asp Gly 
            820                 825                 830         


Glu Phe Val Lys Ser Asn Lys Lys Pro Asn Gly Thr Ser Arg Lys Gln 
        835                 840                 845             


Thr Ala Tyr Ile His Gly Asp Met Phe Ile Asp Ser Ile Ser His Lys 
    850                 855                 860                 


Asn Lys Lys Met Phe Leu Arg Glu Asn Leu Ile Arg Asn Gly Phe Ile 
865                 870                 875                 880 


Ser Lys 
        


<210>  108
<211>  935
<212>  PRT
<213>  ˹

<220>
<223>  Cas12j.2

<400>  108

Met Asn Lys Thr Asp Thr Gln Asn Asn Glu Gln Ile Asn Lys Pro Thr 
1               5                   10                  15      


Gln Leu Leu Asn Asn Lys Asp Ile Glu Leu Thr Val Lys Thr Val Lys 
            20                  25                  30          


Ser Ala Thr Val Lys Val Asp Asn Asn Ser Lys Lys Glu Leu Phe Gly 
        35                  40                  45              


Leu Phe Asn Tyr Phe Thr Ser Val Ala Ser Gly Ile Lys Asp Lys Val 
    50                  55                  60                  


Tyr Asn Leu Gln Ser Asp Glu Lys Thr Ala Pro Ile Phe Asn Asp Tyr 
65                  70                  75                  80  


Val Lys Gln Pro Gln Arg Gly Arg Ser Ala Ala Thr Thr Leu Phe Thr 
                85                  90                  95      


Lys Leu Asp Ala Glu Lys Thr Tyr Thr Ser Gln His Ser Phe Pro Gly 
            100                 105                 110         


Lys Trp Arg Asp Ser Gly Ile Phe Pro Leu Tyr Asn Lys Glu Ser Glu 
        115                 120                 125             


Lys Tyr Asp Leu Ser Thr His Gly Tyr His Tyr Ser Ala Asn Ala Glu 
    130                 135                 140                 


Ile His Thr Gln Leu Asp Ser His Asp Glu Cys Asn Lys Glu Cys Glu 
145                 150                 155                 160 


Lys Glu Tyr Ala Ala Leu Arg Asp Glu Val Asn Asn Tyr Lys Tyr Glu 
                165                 170                 175     


Phe Thr Leu Gln Phe Lys Ala Glu Asn Ala Glu Lys Phe Tyr Asn Phe 
            180                 185                 190         


Val Glu Lys Leu Thr Leu Met Gly Trp Arg Tyr Asp Ala Thr Phe Arg 
        195                 200                 205             


Ser Phe Phe Glu Leu His Met His Pro Lys Leu Lys Thr Gly Glu Thr 
    210                 215                 220                 


Thr Tyr Arg Ala Thr Tyr Lys Leu Pro Ser Gly Lys Ser Lys Arg Tyr 
225                 230                 235                 240 


Ser Phe Phe Arg Asp Asp Ile Ala Asp Glu Ile Ala Lys Asn Pro Glu 
                245                 250                 255     


Phe Trp Pro Met Leu Glu Ser Ser Asn Ala Ile Ser Trp Ile Asn Ser 
            260                 265                 270         


Asn Asn Leu Leu Ser Arg Lys Lys Asp Lys Ala Asn Tyr Ser Ser Thr 
        275                 280                 285             


Ser Leu Ile Lys Ser Gln Ile Arg Leu Tyr Leu Gly Asn Asn Gly Val 
    290                 295                 300                 


Pro Phe Thr Ala Arg Glu His Asp Gly Arg Ile Tyr Phe Ser Phe Arg 
305                 310                 315                 320 


Leu Pro Ala Ile Asn Gly Glu Lys Gly Arg Met Val Glu Ile Pro Cys 
                325                 330                 335     


Ser Tyr Lys Lys Val Phe Asn Gly Lys Ala Arg Lys Ser Cys Tyr Leu 
            340                 345                 350         


Gly Gly Leu Thr Ile Glu Lys Thr Asp Ala Gly Lys His Ile Phe Lys 
        355                 360                 365             


Tyr Ser Val Asn Asn Lys Lys Pro Gln Val Ala Glu Leu Asn Glu Cys 
    370                 375                 380                 


Phe Leu Arg Leu Val Val Arg Asn Arg Glu Tyr Phe Asn Asn Val Val 
385                 390                 395                 400 


Ala Gly Lys Ile Thr Asp Ile Asn Thr Asp His Phe Asp Phe Tyr Val 
                405                 410                 415     


Asp Leu Pro Leu Asn Val Lys Glu Asp Pro Ile His Asp Leu Ser Ser 
            420                 425                 430         


Thr Glu Val Phe Gly Lys Asn Gly Leu Arg Ser Tyr Tyr Ser Ser Ala 
        435                 440                 445             


Tyr Pro Glu Ile Lys Asn Leu Gly Ser Gln Ile Glu Thr Gly Lys Asn 
    450                 455                 460                 


Leu Thr Cys Pro Ile Thr Lys Thr His Asn Ile Met Gly Ile Asp Leu 
465                 470                 475                 480 


Gly Gln Arg Asn Pro Phe Ala Tyr Cys Ile Lys Asp Asn Thr Gly Lys 
                485                 490                 495     


Leu Ile Ala Gln Gly His Met Asp Gly Ser Lys Asn Glu Thr Tyr Lys 
            500                 505                 510         


Lys Tyr Ile Asn Phe Gly Lys Glu Ser Thr Ser Val Ser His Leu Ile 
        515                 520                 525             


Lys Glu Thr Arg Ser Tyr Leu His Gly Asp Pro Glu Ala Ile Ser Lys 
    530                 535                 540                 


Glu Leu Tyr Asn Glu Val Ala Gly Phe Cys Asn Asn Pro Val Ser Tyr 
545                 550                 555                 560 


Glu Glu Tyr Leu Lys Tyr Leu Asp Ser Lys Lys Phe Leu Ile Asn Lys 
                565                 570                 575     


Glu Asp Leu Ser Lys Asn Ala Met His Leu Leu Arg Gln Lys Asp His 
            580                 585                 590         


Asn Trp Ile Gly Arg Asp Trp Leu Trp Tyr Ile Ser Lys Gln Tyr Lys 
        595                 600                 605             


Lys His Asn Glu Asn Arg Met Gln Asp Ala Asp Trp Arg Gln Thr Leu 
    610                 615                 620                 


Tyr Trp Ile Asp Ser Leu Tyr Arg Tyr Ile Asp Val Met Lys Ser Phe 
625                 630                 635                 640 


His Asn Phe Gly Ser Phe Tyr Asp Lys Asn Leu Lys Lys Lys Val Asn 
                645                 650                 655     


Gly Thr Val Val Gly Phe Cys Lys Thr Val His Asp Gln Ile Asn Asn 
            660                 665                 670         


Asn Asn Asp Asp Met Phe Lys Lys Phe Thr Asn Glu Leu Met Ser Val 
        675                 680                 685             


Ile Arg Glu His Lys Val Ser Val Val Ala Leu Glu Lys Met Asp Ser 
    690                 695                 700                 


Met Leu Gly Asp Lys Ser Arg His Thr Phe Glu Asn Arg Asn Tyr Asn 
705                 710                 715                 720 


Leu Trp Pro Val Gly Gln Leu Lys Thr Phe Met Glu Gly Lys Leu Glu 
                725                 730                 735     


Ser Phe Asn Val Ala Leu Ile Glu Ile Asp Glu Arg Asn Thr Ser Gln 
            740                 745                 750         


Val Cys Lys Glu Asn Trp Ser Tyr Arg Glu Ala Asp Asp Leu Tyr Tyr 
        755                 760                 765             


Val Thr Asp Gly Glu Ser His Lys Val His Ala Asp Glu Asn Ala Ala 
    770                 775                 780                 


Asn Asn Ile Val Asp Arg Cys Ile Ser Arg His Thr Asn Met Phe Ser 
785                 790                 795                 800 


Leu His Met Val Asn Pro Lys Asp Asp Tyr Tyr Val Pro Thr Cys Ile 
                805                 810                 815     


Trp Asp Thr Thr Glu Glu Ser Gly Lys Arg Val Arg Gly Phe Leu Thr 
            820                 825                 830         


Lys Leu Tyr Lys Asn Ser Asp Val Val Phe Thr Lys Lys Gly Asp Lys 
        835                 840                 845             


Leu Val Lys Ser Lys Thr Ser Val Lys Glu Leu Lys Lys Leu Val Gly 
    850                 855                 860                 


Lys Thr Lys Glu Lys Arg Gly Gln Tyr Trp Tyr Arg Phe Glu Gly Lys 
865                 870                 875                 880 


Ser Trp Ile Asn Glu Ala Asp Arg Asp Thr Ile Ile Leu Asn Ala Lys 
                885                 890                 895     


Lys Ile Ser Arg Glu Arg Asp Asn Gly Glu Gln Ser Thr Asp Thr Arg 
            900                 905                 910         


Ser Gln Asn Val Thr Val Ser Val Leu Asp Val Cys Glu Thr Ala Glu 
        915                 920                 925             


Lys Lys Lys Leu Val Leu Val 
    930                 935 


