                         SEQUENCE LISTING

<110>  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
       Vale, Ronald D
       Morrisey, Meghan A
       Williamson, Adam P
 
<120>  CHIMERIC ANTIGEN RECEPTORS FOR PHAGOCYTOSIS

<130>  048536-626001WO

<140>  Herewith
<141>  Herewith

<150>  US 62/756,235
<151>  2018-11-06

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  578
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(332)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (333)..(339)
<223>  basic sequence to improve surface expression

<220>
<221>  misc_feature
<222>  (340)..(578)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-empty CAR-P - control, basic region improves
       membrane expression

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
                325                 330                 335     


Arg Arg Arg Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
            340                 345                 350         


Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
        355                 360                 365             


Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
    370                 375                 380                 


Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
385                 390                 395                 400 


Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 
                405                 410                 415     


His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
            420                 425                 430         


Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 
        435                 440                 445             


Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
    450                 455                 460                 


Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
465                 470                 475                 480 


Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys 
                485                 490                 495     


Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu 
            500                 505                 510         


Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
        515                 520                 525             


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 
    530                 535                 540                 


Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
545                 550                 555                 560 


Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
                565                 570                 575     


Tyr Lys 
        


<210>  2
<211>  847
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(332)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (333)..(601)
<223>  native phagocytosis receptor signaling domain

<220>
<221>  misc_feature
<222>  (609)..(847)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-mMegf10 CAR-P

<400>  2

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Tyr Arg His Lys 
                325                 330                 335     


Gln Lys Arg Lys Glu Ser Ser Met Pro Ala Val Thr Tyr Thr Pro Ala 
            340                 345                 350         


Met Arg Val Ile Asn Ala Asp Tyr Thr Ile Ala Glu Thr Leu Pro His 
        355                 360                 365             


Ser Asn Gly Gly Asn Ala Asn Ser His Tyr Phe Thr Asn Pro Ser Tyr 
    370                 375                 380                 


His Thr Leu Ser Gln Cys Ala Thr Ser Pro His Val Asn Asn Arg Asp 
385                 390                 395                 400 


Arg Met Thr Ile Ala Lys Ser Lys Asn Asn Gln Leu Phe Val Asn Leu 
                405                 410                 415     


Lys Asn Val Asn Pro Gly Lys Arg Gly Thr Leu Val Asp Cys Thr Gly 
            420                 425                 430         


Thr Leu Pro Ala Asp Trp Lys Gln Gly Gly Tyr Leu Asn Glu Leu Gly 
        435                 440                 445             


Ala Phe Gly Leu Asp Arg Ser Tyr Met Gly Lys Ser Leu Lys Asp Leu 
    450                 455                 460                 


Gly Lys Asn Ser Glu Tyr Asn Ser Ser Thr Cys Ser Leu Ser Ser Ser 
465                 470                 475                 480 


Glu Asn Pro Tyr Ala Thr Ile Lys Asp Pro Pro Ala Leu Leu Pro Lys 
                485                 490                 495     


Ser Ser Glu Cys Gly Tyr Val Glu Met Lys Ser Pro Ala Arg Arg Asp 
            500                 505                 510         


Ser Pro Tyr Ala Glu Ile Asn Asn Ser Thr Pro Ala Asn Arg Asn Val 
        515                 520                 525             


Tyr Glu Val Glu Pro Thr Val Ser Val Val Gln Gly Val Phe Ser Asn 
    530                 535                 540                 


Ser Gly His Val Thr Gln Asp Pro Tyr Asp Leu Pro Lys Asn Ser His 
545                 550                 555                 560 


Ile Pro Cys His Tyr Asp Leu Leu Pro Val Arg Asp Ser Ser Ser Ser 
                565                 570                 575     


Pro Lys Arg Glu Asp Gly Gly Gly Ser Asn Ser Thr Ser Ser Asn Ser 
            580                 585                 590         


Thr Ser Ser Ser Ser Ser Ser Ser Glu Ala Asp Pro Pro Val Ala Thr 
        595                 600                 605             


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
    610                 615                 620                 


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
625                 630                 635                 640 


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
                645                 650                 655     


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
            660                 665                 670         


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
        675                 680                 685             


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
    690                 695                 700                 


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
705                 710                 715                 720 


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
                725                 730                 735     


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
            740                 745                 750         


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
        755                 760                 765             


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
    770                 775                 780                 


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
785                 790                 795                 800 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu 
                805                 810                 815     


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
            820                 825                 830         


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        835                 840                 845         


<210>  3
<211>  618
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(332)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (334)..(377)
<223>  native phagocytosis receptor signaling domain

<220>
<221>  misc_feature
<222>  (380)..(618)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-FcGamma CAR-P - Native receptor (non-optimized)

<400>  3

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Arg Leu Lys 
                325                 330                 335     


Ile Gln Val Arg Lys Ala Ala Ile Ala Ser Arg Glu Lys Ala Asp Ala 
            340                 345                 350         


Val Tyr Thr Gly Leu Asn Thr Arg Ser Gln Glu Thr Tyr Glu Thr Leu 
        355                 360                 365             


Lys His Glu Lys Pro Pro Gln Gly Ser Gly Ser Met Val Ser Lys Gly 
    370                 375                 380                 


Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 
385                 390                 395                 400 


Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 
                405                 410                 415     


Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 
            420                 425                 430         


Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val 
        435                 440                 445             


Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe 
    450                 455                 460                 


Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe 
465                 470                 475                 480 


Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 
                485                 490                 495     


Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 
            500                 505                 510         


Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 
        515                 520                 525             


Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn 
    530                 535                 540                 


Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp 
545                 550                 555                 560 


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
                565                 570                 575     


Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn 
            580                 585                 590         


Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
        595                 600                 605             


Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    610                 615             


<210>  4
<211>  620
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(332)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (333)..(339)
<223>  basic sequence to improve surface expression

<220>
<221>  misc_feature
<222>  (345)..(379)
<223>  PI3K recruitment domain

<220>
<221>  misc_feature
<222>  (387)..(620)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-PI3K CAR-P - Rationally designed ingest large targets

<400>  4

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
                325                 330                 335     


Arg Arg Arg Gly Ser Gly Gly Ser Tyr Glu Asp Met Arg Gly Ile Leu 
            340                 345                 350         


Tyr Ala Ala Pro Gln Leu Arg Ser Ile Arg Gly Gln Pro Gly Pro Asn 
        355                 360                 365             


His Glu Glu Asp Ala Asp Ser Tyr Glu Asn Met Ser Gly Gly Ser Gly 
    370                 375                 380                 


Ser Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
385                 390                 395                 400 


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
                405                 410                 415     


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
            420                 425                 430         


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
        435                 440                 445             


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
    450                 455                 460                 


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
465                 470                 475                 480 


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
                485                 490                 495     


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
            500                 505                 510         


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
        515                 520                 525             


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
    530                 535                 540                 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
545                 550                 555                 560 


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
                565                 570                 575     


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
            580                 585                 590         


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
        595                 600                 605             


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
    610                 615                 620 


<210>  5
<211>  656
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(332)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (334)..(377)
<223>  native phagocytosis receptor signaling domain

<220>
<221>  misc_feature
<222>  (380)..(413)
<223>  PI3K recruitment domain

<220>
<221>  misc_feature
<222>  (419)..(656)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-tandem CAR-P - Optimized CAR-P with tandem signaling
       domains (Fc-gamma and PI3K in a linear array)

<400>  5

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Arg Leu Lys 
                325                 330                 335     


Ile Gln Val Arg Lys Ala Ala Ile Ala Ser Arg Glu Lys Ala Asp Ala 
            340                 345                 350         


Val Tyr Thr Gly Leu Asn Thr Arg Ser Gln Glu Thr Tyr Glu Thr Leu 
        355                 360                 365             


Lys His Glu Lys Pro Pro Gln Gly Ser Gly Ser Tyr Glu Asp Met Arg 
    370                 375                 380                 


Gly Ile Leu Tyr Ala Ala Pro Gln Leu Arg Ser Ile Arg Gly Gln Pro 
385                 390                 395                 400 


Gly Pro Asn His Glu Glu Asp Ala Asp Ser Tyr Glu Asn Met Gly Ser 
                405                 410                 415     


Gly Ser Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro 
            420                 425                 430         


Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
        435                 440                 445             


Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys 
    450                 455                 460                 


Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val 
465                 470                 475                 480 


Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His 
                485                 490                 495     


Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val 
            500                 505                 510         


Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg 
        515                 520                 525             


Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu 
    530                 535                 540                 


Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu 
545                 550                 555                 560 


Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln 
                565                 570                 575     


Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp 
            580                 585                 590         


Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
        595                 600                 605             


Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser 
    610                 615                 620                 


Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu 
625                 630                 635                 640 


Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr 
                645                 650                 655     


<210>  6
<211>  842
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(258)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (260)..(327)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (328)..(596)
<223>  native phagocytosis receptor signaling domain

<220>
<221>  misc_feature
<222>  (604)..(842)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD22-Megf10 CAR-P

<400>  6

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly 
        35                  40                  45              


Asp Ser Val Ser Ser Asn Ser Ala Ala Trp Asn Trp Ile Arg Gln Ser 
    50                  55                  60                  


Pro Ser Arg Gly Leu Glu Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys 
65                  70                  75                  80  


Trp Tyr Asn Asp Tyr Ala Val Ser Val Lys Ser Arg Ile Thr Ile Asn 
                85                  90                  95      


Pro Asp Thr Ser Lys Asn Gln Phe Ser Leu Gln Leu Asn Ser Val Thr 
            100                 105                 110         


Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Val Thr Gly Asp 
        115                 120                 125             


Leu Glu Asp Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser 
145                 150                 155                 160 


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
                165                 170                 175     


Ser Gln Thr Ile Trp Ser Tyr Leu Asn Trp Tyr Gln Gln Arg Pro Gly 
            180                 185                 190         


Lys Ala Pro Asn Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly 
        195                 200                 205             


Val Pro Ser Arg Phe Ser Gly Arg Gly Ser Gly Thr Asp Phe Thr Leu 
    210                 215                 220                 


Thr Ile Ser Ser Leu Gln Ala Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
225                 230                 235                 240 


Gln Ser Tyr Ser Ile Pro Gln Thr Phe Gly Gln Gly Thr Lys Leu Glu 
                245                 250                 255     


Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
            260                 265                 270         


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
        275                 280                 285             


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
    290                 295                 300                 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
305                 310                 315                 320 


Leu Val Ile Thr Leu Tyr Cys Tyr Arg His Lys Gln Lys Arg Lys Glu 
                325                 330                 335     


Ser Ser Met Pro Ala Val Thr Tyr Thr Pro Ala Met Arg Val Ile Asn 
            340                 345                 350         


Ala Asp Tyr Thr Ile Ala Glu Thr Leu Pro His Ser Asn Gly Gly Asn 
        355                 360                 365             


Ala Asn Ser His Tyr Phe Thr Asn Pro Ser Tyr His Thr Leu Ser Gln 
    370                 375                 380                 


Cys Ala Thr Ser Pro His Val Asn Asn Arg Asp Arg Met Thr Ile Ala 
385                 390                 395                 400 


Lys Ser Lys Asn Asn Gln Leu Phe Val Asn Leu Lys Asn Val Asn Pro 
                405                 410                 415     


Gly Lys Arg Gly Thr Leu Val Asp Cys Thr Gly Thr Leu Pro Ala Asp 
            420                 425                 430         


Trp Lys Gln Gly Gly Tyr Leu Asn Glu Leu Gly Ala Phe Gly Leu Asp 
        435                 440                 445             


Arg Ser Tyr Met Gly Lys Ser Leu Lys Asp Leu Gly Lys Asn Ser Glu 
    450                 455                 460                 


Tyr Asn Ser Ser Thr Cys Ser Leu Ser Ser Ser Glu Asn Pro Tyr Ala 
465                 470                 475                 480 


Thr Ile Lys Asp Pro Pro Ala Leu Leu Pro Lys Ser Ser Glu Cys Gly 
                485                 490                 495     


Tyr Val Glu Met Lys Ser Pro Ala Arg Arg Asp Ser Pro Tyr Ala Glu 
            500                 505                 510         


Ile Asn Asn Ser Thr Pro Ala Asn Arg Asn Val Tyr Glu Val Glu Pro 
        515                 520                 525             


Thr Val Ser Val Val Gln Gly Val Phe Ser Asn Ser Gly His Val Thr 
    530                 535                 540                 


Gln Asp Pro Tyr Asp Leu Pro Lys Asn Ser His Ile Pro Cys His Tyr 
545                 550                 555                 560 


Asp Leu Leu Pro Val Arg Asp Ser Ser Ser Ser Pro Lys Arg Glu Asp 
                565                 570                 575     


Gly Gly Gly Ser Asn Ser Thr Ser Ser Asn Ser Thr Ser Ser Ser Ser 
            580                 585                 590         


Ser Ser Ser Glu Ala Asp Pro Pro Val Ala Thr Met Val Ser Lys Gly 
        595                 600                 605             


Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 
    610                 615                 620                 


Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 
625                 630                 635                 640 


Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 
                645                 650                 655     


Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val 
            660                 665                 670         


Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe 
        675                 680                 685             


Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe 
    690                 695                 700                 


Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 
705                 710                 715                 720 


Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 
                725                 730                 735     


Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 
            740                 745                 750         


Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn 
        755                 760                 765             


Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp 
    770                 775                 780                 


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
785                 790                 795                 800 


Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn 
                805                 810                 815     


Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
            820                 825                 830         


Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        835                 840         


<210>  7
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(258)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (260)..(327)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (328)..(334)
<223>  basic sequence to improve surface expression

<220>
<221>  misc_feature
<222>  (335)..(573)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD22-empty CAR-P - control, baisc region improves 
       membrane expression

<400>  7

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly 
        35                  40                  45              


Asp Ser Val Ser Ser Asn Ser Ala Ala Trp Asn Trp Ile Arg Gln Ser 
    50                  55                  60                  


Pro Ser Arg Gly Leu Glu Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys 
65                  70                  75                  80  


Trp Tyr Asn Asp Tyr Ala Val Ser Val Lys Ser Arg Ile Thr Ile Asn 
                85                  90                  95      


Pro Asp Thr Ser Lys Asn Gln Phe Ser Leu Gln Leu Asn Ser Val Thr 
            100                 105                 110         


Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Val Thr Gly Asp 
        115                 120                 125             


Leu Glu Asp Ala Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser 
145                 150                 155                 160 


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
                165                 170                 175     


Ser Gln Thr Ile Trp Ser Tyr Leu Asn Trp Tyr Gln Gln Arg Pro Gly 
            180                 185                 190         


Lys Ala Pro Asn Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly 
        195                 200                 205             


Val Pro Ser Arg Phe Ser Gly Arg Gly Ser Gly Thr Asp Phe Thr Leu 
    210                 215                 220                 


Thr Ile Ser Ser Leu Gln Ala Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
225                 230                 235                 240 


Gln Ser Tyr Ser Ile Pro Gln Thr Phe Gly Gln Gly Thr Lys Leu Glu 
                245                 250                 255     


Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
            260                 265                 270         


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
        275                 280                 285             


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
    290                 295                 300                 


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
305                 310                 315                 320 


Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Met Val 
                325                 330                 335     


Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
            340                 345                 350         


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly 
        355                 360                 365             


Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
    370                 375                 380                 


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr 
385                 390                 395                 400 


Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His 
                405                 410                 415     


Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr 
            420                 425                 430         


Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys 
        435                 440                 445             


Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp 
    450                 455                 460                 


Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr 
465                 470                 475                 480 


Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile 
                485                 490                 495     


Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln 
            500                 505                 510         


Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val 
        515                 520                 525             


Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys 
    530                 535                 540                 


Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr 
545                 550                 555                 560 


Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                565                 570             


<210>  8
<211>  1054
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(332)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (333)..(808)
<223>  native phagocytosis receptor signaling domain

<220>
<221>  misc_feature
<222>  (816)..(1054)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-MerTK CAR-P - Poor CAR-P (no ITAM domain)

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Ala Leu Arg Arg 
                325                 330                 335     


Arg Val Gln Glu Thr Lys Phe Gly Gly Ala Phe Ser Glu Glu Asp Ser 
            340                 345                 350         


Gln Leu Val Val Asn Tyr Arg Ala Lys Lys Ser Phe Cys Arg Arg Ala 
        355                 360                 365             


Ile Glu Leu Thr Leu Gln Ser Leu Gly Val Ser Glu Glu Leu Gln Asn 
    370                 375                 380                 


Lys Leu Glu Asp Val Val Ile Asp Arg Asn Leu Leu Val Leu Gly Lys 
385                 390                 395                 400 


Val Leu Gly Glu Gly Glu Phe Gly Ser Val Met Glu Gly Asn Leu Lys 
                405                 410                 415     


Gln Glu Asp Gly Thr Ser Gln Lys Val Ala Val Lys Thr Met Lys Leu 
            420                 425                 430         


Asp Asn Phe Ser Gln Arg Glu Ile Glu Glu Phe Leu Ser Glu Ala Ala 
        435                 440                 445             


Cys Met Lys Asp Phe Asn His Pro Asn Val Ile Arg Leu Leu Gly Val 
    450                 455                 460                 


Cys Ile Glu Leu Ser Ser Gln Gly Ile Pro Lys Pro Met Val Ile Leu 
465                 470                 475                 480 


Pro Phe Met Lys Tyr Gly Asp Leu His Thr Phe Leu Leu Tyr Ser Arg 
                485                 490                 495     


Leu Asn Thr Gly Pro Lys Tyr Ile His Leu Gln Thr Leu Leu Lys Phe 
            500                 505                 510         


Met Met Asp Ile Ala Gln Gly Met Glu Tyr Leu Ser Asn Arg Asn Phe 
        515                 520                 525             


Leu His Arg Asp Leu Ala Ala Arg Asn Cys Met Leu Arg Asp Asp Met 
    530                 535                 540                 


Thr Val Cys Val Ala Asp Phe Gly Leu Ser Lys Lys Ile Tyr Ser Gly 
545                 550                 555                 560 


Asp Tyr Tyr Arg Gln Gly Arg Ile Ala Lys Met Pro Val Lys Trp Ile 
                565                 570                 575     


Ala Ile Glu Ser Leu Ala Asp Arg Val Tyr Thr Ser Lys Ser Asp Val 
            580                 585                 590         


Trp Ala Phe Gly Val Thr Met Trp Glu Ile Thr Thr Arg Gly Met Thr 
        595                 600                 605             


Pro Tyr Pro Gly Val Gln Asn His Glu Met Tyr Asp Tyr Leu Leu His 
    610                 615                 620                 


Gly His Arg Leu Lys Gln Pro Glu Asp Cys Leu Asp Glu Leu Tyr Asp 
625                 630                 635                 640 


Ile Met Tyr Ser Cys Trp Ser Ala Asp Pro Leu Asp Arg Pro Thr Phe 
                645                 650                 655     


Ser Val Leu Arg Leu Gln Leu Glu Lys Leu Ser Glu Ser Leu Pro Asp 
            660                 665                 670         


Ala Gln Asp Lys Glu Ser Ile Ile Tyr Ile Asn Thr Gln Leu Leu Glu 
        675                 680                 685             


Ser Cys Glu Gly Ile Ala Asn Gly Pro Ser Leu Thr Gly Leu Asp Met 
    690                 695                 700                 


Asn Ile Asp Pro Asp Ser Ile Ile Ala Ser Cys Thr Pro Gly Ala Ala 
705                 710                 715                 720 


Val Ser Val Val Thr Ala Glu Val His Glu Asn Asn Leu Arg Glu Glu 
                725                 730                 735     


Arg Tyr Ile Leu Asn Gly Gly Asn Glu Glu Trp Glu Asp Val Ser Ser 
            740                 745                 750         


Thr Pro Phe Ala Ala Val Thr Pro Glu Lys Asp Gly Val Leu Pro Glu 
        755                 760                 765             


Asp Arg Leu Thr Lys Asn Gly Val Ser Trp Ser His His Ser Thr Leu 
    770                 775                 780                 


Pro Leu Gly Ser Pro Ser Pro Asp Glu Leu Leu Phe Val Asp Asp Ser 
785                 790                 795                 800 


Leu Glu Asp Ser Glu Val Leu Met Thr Ser Gly Gly Ser Gly Ser Met 
                805                 810                 815     


Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
            820                 825                 830         


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
        835                 840                 845             


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
    850                 855                 860                 


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
865                 870                 875                 880 


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
                885                 890                 895     


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
            900                 905                 910         


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
        915                 920                 925             


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
    930                 935                 940                 


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
945                 950                 955                 960 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
                965                 970                 975     


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
            980                 985                 990         


Gln Leu Ala Asp His Tyr Gln Gln  Asn Thr Pro Ile Gly  Asp Gly Pro 
        995                 1000                 1005             


Val Leu  Leu Pro Asp Asn His  Tyr Leu Ser Thr Gln  Ser Lys Leu 
    1010                 1015                 1020             


Ser Lys  Asp Pro Asn Glu Lys  Arg Asp His Met Val  Leu Leu Glu 
    1025                 1030                 1035             


Phe Val  Thr Ala Ala Gly Ile  Thr Leu Gly Met Asp  Glu Leu Tyr 
    1040                 1045                 1050             


Lys 
    


<210>  9
<211>  972
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (23)..(263)
<223>  extracellular antigen-binding sequence

<220>
<221>  misc_feature
<222>  (265)..(320)
<223>  stalk/transmembrane domain for plasma insertion

<220>
<221>  misc_feature
<222>  (321)..(729)
<223>  native phagocytosis receptor signaling domain

<220>
<221>  misc_feature
<222>  (734)..(972)
<223>  fluophore

<220>
<221>  misc_feature
<223>  CD19-Bai1 CAR-P

<400>  9

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Arg Glu Val 
                325                 330                 335     


Gln Asp Ala Val Lys Cys Arg Val Val Asp Arg Gln Glu Glu Gly Asn 
            340                 345                 350         


Gly Asp Ser Gly Gly Ser Phe Gln Asn Gly His Ala Gln Leu Met Thr 
        355                 360                 365             


Asp Phe Glu Lys Asp Val Asp Leu Ala Cys Arg Ser Val Leu Asn Lys 
    370                 375                 380                 


Asp Ile Ala Ala Cys Arg Thr Ala Thr Ile Thr Gly Thr Leu Lys Arg 
385                 390                 395                 400 


Pro Ser Leu Pro Glu Glu Glu Lys Leu Lys Leu Ala His Ala Lys Gly 
                405                 410                 415     


Pro Pro Thr Asn Phe Asn Ser Leu Pro Ala Asn Val Ser Lys Leu His 
            420                 425                 430         


Leu His Gly Ser Pro Arg Tyr Pro Gly Gly Pro Leu Pro Asp Phe Pro 
        435                 440                 445             


Asn His Ser Leu Thr Leu Lys Arg Asp Lys Ala Pro Lys Ser Ser Phe 
    450                 455                 460                 


Val Gly Asp Gly Asp Ile Phe Lys Lys Leu Asp Ser Glu Leu Ser Arg 
465                 470                 475                 480 


Ala Gln Glu Lys Ala Leu Asp Thr Ser Tyr Val Ile Leu Pro Thr Ala 
                485                 490                 495     


Thr Ala Thr Leu Arg Pro Lys Pro Lys Glu Glu Pro Lys Tyr Ser Ile 
            500                 505                 510         


His Ile Asp Gln Met Pro Gln Thr Arg Leu Ile His Leu Ser Thr Ala 
        515                 520                 525             


Pro Glu Ala Ser Leu Pro Ala Arg Ser Pro Pro Ser Arg Gln Pro Pro 
    530                 535                 540                 


Ser Gly Gly Pro Pro Glu Ala Pro Pro Ala Gln Pro Pro Pro Pro Pro 
545                 550                 555                 560 


Pro Pro Pro Pro Pro Pro Pro Gln Gln Pro Leu Pro Pro Pro Pro Asn 
                565                 570                 575     


Leu Glu Pro Ala Pro Pro Ser Leu Gly Asp Pro Gly Glu Pro Ala Ala 
            580                 585                 590         


His Pro Gly Pro Ser Thr Gly Pro Ser Thr Lys Asn Glu Asn Val Ala 
        595                 600                 605             


Thr Leu Ser Val Ser Ser Leu Glu Arg Arg Lys Ser Arg Tyr Ala Glu 
    610                 615                 620                 


Leu Asp Phe Glu Lys Ile Met His Thr Arg Lys Arg His Gln Asp Met 
625                 630                 635                 640 


Phe Gln Asp Leu Asn Arg Lys Leu Gln His Ala Ala Glu Lys Asp Lys 
                645                 650                 655     


Glu Val Leu Gly Pro Asp Ser Lys Pro Glu Lys Gln Gln Thr Pro Asn 
            660                 665                 670         


Lys Arg Pro Trp Glu Ser Leu Arg Lys Ala His Gly Thr Pro Thr Trp 
        675                 680                 685             


Val Lys Lys Glu Leu Glu Pro Leu Gln Pro Ser Pro Leu Glu Leu Arg 
    690                 695                 700                 


Ser Val Glu Trp Glu Arg Ser Gly Ala Thr Ile Pro Leu Val Gly Gln 
705                 710                 715                 720 


Asp Ile Ile Asp Leu Gln Thr Glu Val Gly Ser Gly Ser Met Val Ser 
                725                 730                 735     


Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
            740                 745                 750         


Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
        755                 760                 765             


Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
    770                 775                 780                 


Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
785                 790                 795                 800 


Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
                805                 810                 815     


Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
            820                 825                 830         


Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
        835                 840                 845             


Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
    850                 855                 860                 


Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
865                 870                 875                 880 


Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
                885                 890                 895     


Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
            900                 905                 910         


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
        915                 920                 925             


Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp 
    930                 935                 940                 


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
945                 950                 955                 960 


Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                965                 970         


<210>  10
<211>  1737
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-empty CAR-P - Control, basic region improves membrane expression

<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 1

<400>  10
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgcaacc acaggaaccg aagacgtatg     1020

gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc     1080

gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc     1140

aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc     1200

gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag     1260

cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc     1320

aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg     1380

aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag     1440

ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc     1500

atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac     1560

cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac     1620

ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg     1680

ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa        1737


<210>  11
<211>  2544
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-mMegf10 CAR-P

<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 2

<400>  11
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgctaca gacacaagca gaagaggaag     1020

gaatcaagca tgccggccgt gacctacacc cccgccatga gagtcatcaa tgcagactat     1080

accatcgcag aaaccctgcc tcacagcaat ggtggaaatg ccaacagcca ctactttacc     1140

aatcccagtt atcacacact tagccagtgt gccacatccc ctcatgtgaa caatagggac     1200

aggatgacca ttgcaaagtc aaaaaacaat cagctgtttg tgaatcttaa aaatgtgaat     1260

ccagggaaga gagggacatt ggtggactgc actgggacat tgccagctga ctggaagcaa     1320

ggaggctacc tcaatgagct tggtgctttc gggctggaca gaagctacat gggaaagtcc     1380

ttaaaagatc tggggaagaa ctctgaatat aattcaagca cttgctcctt aagcagctct     1440

gaaaacccat atgccaccat taaagacccg cctgcactcc tgcctaaaag ctccgagtgc     1500

ggctacgtgg agatgaagtc gccggcgcgg agagactccc catatgcaga gatcaacaac     1560

tcaactccag ccaacaggaa tgtctatgaa gtcgaaccta cagtgagcgt tgtgcaagga     1620

gtattcagca acagcggtca cgtcacccaa gacccatatg accttccaaa gaacagtcac     1680

atcccttgcc attatgacct gctgccagta agggacagtt catcctcccc aaagagagag     1740

gatggtggtg gcagcaacag caccagcagc aacagcacca gcagcagcag cagcagcagt     1800

gaagcggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg     1860

gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc     1920

gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc     1980

aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc     2040

agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc     2100

tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag     2160

gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag     2220

gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat     2280

atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc     2340

gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc     2400

cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc     2460

aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc     2520

ggcatggacg agctgtacaa gtaa                                            2544


<210>  12
<211>  1832
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-FcGamma CAR-P - Native receptor (non-optimized)

<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 3

<400>  12
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcag gcggtggcgg ttcgggtggc ggcgggtcgg gcggtggagg ctctctggga      840

gagccgcagc tctgctatat cctggatgct gtcctgtttt tgtatggtat tgtccttacc      900

ctactctact gtcgactcaa gatccaggtc cgaaaggcag ctatagccag ccgtgagaaa      960

gcagatgctg tctacacggg cctgaacacc cggagccagg agacatatga gactctgaag     1020

catgagaaac caccccaggg atccggaagt atggtgagca agggcgagga gctgttcacc     1080

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     1140

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     1200

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag     1260

tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     1320

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     1380

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     1440

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     1500

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     1560

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     1620

gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa     1680

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     1740

actctcggca tggacgagct gtacaagtaa agcggccgcg actctagagt cgacctgcag     1800

gcatgcaagc ttgatatcaa gcttatcgat aa                                   1832


<210>  13
<211>  1863
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-PI3K CAR-P

<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 4

<400>  13
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgcaacc acaggaaccg aagacgtgga     1020

tccggaggca gttatgagga tatgagagga atcctgtatg cagcccccca gctccgctcc     1080

attcggggcc agcctggacc caatcatgag gaagatgcag actcttatga gaacatgagt     1140

ggtggatccg gaagtgtgag caagggcgag gaggataaca tggccatcat caaggagttc     1200

atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc     1260

gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt     1320

ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc     1380

tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc     1440

aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc     1500

tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc     1560

gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac     1620

cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc     1680

cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc     1740

gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg     1800

gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag     1860

taa                                                                   1863


<210>  14
<211>  1974
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-tandem CAR-P

<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 5

<400>  14
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgcagac gactcaagat ccaggtccga     1020

aaggcagcta tagccagccg tgagaaagca gatgctgtct acacgggcct gaacacccgg     1080

agccaggaga catatgagac tctgaagcat gagaaaccac cccagggatc cggaagttat     1140

gaggatatga gaggaatcct gtatgcagcc ccccagctcc gctccattcg gggccagcct     1200

ggacccaatc atgaggaaga tgcagactct tatgagaaca tgggatccgg aagtatggtg     1260

agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac     1320

gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag     1380

ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg     1440

accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac     1500

gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag     1560

gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac     1620

cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg     1680

gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc     1740

aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac     1800

taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg     1860

agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg     1920

gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaa           1974


<210>  15
<211>  2529
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD22-Megf10 CAR-P

<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 6

<400>  15
atgcttctgc tcgtgacaag cctgctgctg tgcgagctgc cccaccctgc ctttctgctg       60

atccctcagg tgcagctgca gcagtctggc cctggcctcg tgaagcctag ccagaccctg      120

agcctgacct gtgccatcag cggcgatagc gtgtccagca atagcgccgc ctggaactgg      180

atcagacaga gccctagcag aggcctggaa tggctgggcc ggacctacta ccggtccaag      240

tggtacaacg actacgccgt gtccgtgaag tcccggatca ccatcaaccc cgacaccagc      300

aagaaccagt tctccctgca gctgaacagc gtgacccccg aggataccgc cgtgtactac      360

tgcgccagag aagtgaccgg cgacctggaa gatgccttcg acatctgggg ccagggcaca      420

atggtcaccg tgtctagcgg aggcggcgga agcgacatcc agatgacaca gagccccagc      480

tccctgagcg ccagcgtggg agacagagtg accatcacct gtcgggccag ccagaccatc      540

tggtcctacc tgaactggta tcagcagcgg cctggcaagg cccccaacct gctgatctat      600

gccgccagct cactgcagag cggcgtgccc agcagatttt ccggcagagg cagcggcacc      660

gacttcaccc tgacaatcag ttccctgcag gccgaggact tcgccaccta ctactgccag      720

cagagctaca gcatccccca gaccttcggc caggggacca agctggaaat caaaaccacg      780

acgccagcgc cgcgaccacc aacaccggcg cccaccatcg cgtcgcagcc cctgtccctg      840

cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc acacgagggg gctggacttc      900

gcctgtgata tctacatctg ggcgcccttg gccgggactt gtggggtcct tctcctgtca      960

ctggttatca ccctttactg ctacagacac aagcagaaga ggaaggaatc aagcatgccg     1020

gccgtgacct acacccccgc catgagagtc atcaatgcag actataccat cgcagaaacc     1080

ctgcctcaca gcaatggtgg aaatgccaac agccactact ttaccaatcc cagttatcac     1140

acacttagcc agtgtgccac atcccctcat gtgaacaata gggacaggat gaccattgca     1200

aagtcaaaaa acaatcagct gtttgtgaat cttaaaaatg tgaatccagg gaagagaggg     1260

acattggtgg actgcactgg gacattgcca gctgactgga agcaaggagg ctacctcaat     1320

gagcttggtg ctttcgggct ggacagaagc tacatgggaa agtccttaaa agatctgggg     1380

aagaactctg aatataattc aagcacttgc tccttaagca gctctgaaaa cccatatgcc     1440

accattaaag acccgcctgc actcctgcct aaaagctccg agtgcggcta cgtggagatg     1500

aagtcgccgg cgcggagaga ctccccatat gcagagatca acaactcaac tccagccaac     1560

aggaatgtct atgaagtcga acctacagtg agcgttgtgc aaggagtatt cagcaacagc     1620

ggtcacgtca cccaagaccc atatgacctt ccaaagaaca gtcacatccc ttgccattat     1680

gacctgctgc cagtaaggga cagttcatcc tccccaaaga gagaggatgg tggtggcagc     1740

aacagcacca gcagcaacag caccagcagc agcagcagca gcagtgaagc ggatccaccg     1800

gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc     1860

gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat     1920

gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc     1980

tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac     2040

cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc     2100

accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc     2160

gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc     2220

ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag     2280

cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg     2340

cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc     2400

gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat     2460

cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg     2520

tacaagtaa                                                             2529


<210>  16
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD22-empty CAR-P
<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 7

<400>  16
atgcttctgc tcgtgacaag cctgctgctg tgcgagctgc cccaccctgc ctttctgctg       60

atccctcagg tgcagctgca gcagtctggc cctggcctcg tgaagcctag ccagaccctg      120

agcctgacct gtgccatcag cggcgatagc gtgtccagca atagcgccgc ctggaactgg      180

atcagacaga gccctagcag aggcctggaa tggctgggcc ggacctacta ccggtccaag      240

tggtacaacg actacgccgt gtccgtgaag tcccggatca ccatcaaccc cgacaccagc      300

aagaaccagt tctccctgca gctgaacagc gtgacccccg aggataccgc cgtgtactac      360

tgcgccagag aagtgaccgg cgacctggaa gatgccttcg acatctgggg ccagggcaca      420

atggtcaccg tgtctagcgg aggcggcgga agcgacatcc agatgacaca gagccccagc      480

tccctgagcg ccagcgtggg agacagagtg accatcacct gtcgggccag ccagaccatc      540

tggtcctacc tgaactggta tcagcagcgg cctggcaagg cccccaacct gctgatctat      600

gccgccagct cactgcagag cggcgtgccc agcagatttt ccggcagagg cagcggcacc      660

gacttcaccc tgacaatcag ttccctgcag gccgaggact tcgccaccta ctactgccag      720

cagagctaca gcatccccca gaccttcggc caggggacca agctggaaat caaaaccacg      780

acgccagcgc cgcgaccacc aacaccggcg cccaccatcg cgtcgcagcc cctgtccctg      840

cgcccagagg cgtgccggcc agcggcgggg ggcgcagtgc acacgagggg gctggacttc      900

gcctgtgata tctacatctg ggcgcccttg gccgggactt gtggggtcct tctcctgtca      960

ctggttatca ccctttactg caaccacagg aaccgaagac gtatggtgag caagggcgag     1020

gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac     1080

aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag     1140

ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc     1200

tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag     1260

tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac     1320

tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg     1380

aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac     1440

aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc     1500

aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac     1560

acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc     1620

aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc     1680

gccgccggga tcactctcgg catggacgag ctgtacaagt aa                        1722


<210>  17
<211>  3165
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-MerTK CAR-P
<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 8

<400>  17
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgcgccc tcagaaggag agtccaggaa     1020

acaaagtttg ggggagcatt ctctgaggag gattcccaac tggtcgtaaa ttatagagcg     1080

aagaagtcct tctgccggcg agccatcgag cttaccttgc agagcctggg agtgagcgag     1140

gagctgcaga ataagctgga agatgttgtg attgacagaa accttctggt tctcggcaaa     1200

gttctgggtg aaggagagtt tgggtctgta atggaaggaa atttgaagca agaagatggg     1260

acttctcaga aggtggcagt gaagaccatg aagttggaca acttttctca acgggagatc     1320

gaggagtttc tcagcgaagc agcatgcatg aaagacttca accacccaaa tgtcatccga     1380

cttctaggcg tgtgtataga actgagctct caaggcatcc cgaagcccat ggtgatttta     1440

cccttcatga aatacggaga cctccacacc ttcctgttat attcccgatt aaacacagga     1500

cccaagtaca ttcacctgca gacactactg aagttcatga tggacattgc ccagggaatg     1560

gagtatctga gcaacaggaa ttttcttcat agggatttgg cagctcgaaa ctgcatgttg     1620

cgggatgaca tgactgtctg cgtggcagac tttggcctct caaagaagat ttacagtggt     1680

gattattacc gccaaggccg cattgccaaa atgcctgtga agtggatcgc catcgagagc     1740

ctggcggacc gagtctacac aagcaaaagt gacgtgtggg cttttggcgt gaccatgtgg     1800

gaaataacaa cacggggaat gactccctat cccggagttc agaaccatga gatgtacgac     1860

taccttctcc acggccacag gctgaagcag cctgaggact gcttggatga actgtatgac     1920

atcatgtact cttgctggag tgctgatccc ttggatcgac ccaccttctc tgtgttgagg     1980

ctgcagctgg aaaagctctc cgagagtttg cctgatgcgc aggacaaaga atccatcatc     2040

tacatcaata cccagttgct agagagctgc gagggcatag ccaatgggcc ctcactcacg     2100

gggctagaca tgaacattga ccctgactcc atcattgcct cttgcacacc aggcgctgcc     2160

gtcagcgtgg tcacggcaga agttcacgag aacaaccttc gtgaggaaag atacatcttg     2220

aatgggggca atgaggaatg ggaagatgtg tcctccactc cttttgctgc agtcacacct     2280

gaaaaggatg gtgtcttacc ggaggacaga ctcaccaaaa atggcgtctc ctggtctcac     2340

catagtacac tacccttggg gagcccatca ccagatgaac ttttatttgt agatgactcc     2400

ttggaagact ctgaagttct gatgactagt ggtggatccg gaagtatggt gagcaagggc     2460

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc     2520

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg     2580

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg     2640

acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc     2700

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc     2760

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag     2820

ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac     2880

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac     2940

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     3000

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     3060

tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     3120

accgccgccg ggatcactct cggcatggac gagctgtaca agtaa                     3165


<210>  18
<211>  2919
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  misc_feature
<223>  CD19-Bai1 CAR-P
<220>
<221>  misc_feature
<223>  encodes the polypeptide of SEQE ID NO: 9

<400>  18
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgtcg      840

cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg      900

agggggctgg acttcgcctg tgatatctac atctgggcgc ccttggccgg gacttgtggg      960

gtccttctcc tgtcactggt tatcaccctt tactgcagaa gagaagtcca ggacgccgtc     1020

aaatgtcgag tggtagacag gcaggaggag ggtaacggtg attctggtgg gtcctttcag     1080

aacggccacg ctcagctcat gaccgatttt gagaaagacg tggatctggc atgtagaagt     1140

gtactgaaca aggacatcgc cgcatgtcgc actgcaacaa taacaggtac tctcaaacgg     1200

ccaagcctcc ccgaagaaga aaaacttaag ctggcacacg ccaagggccc accaacaaac     1260

ttcaatagtc tgccagctaa cgtgagtaag ctccatctcc atggcagtcc ccgctatcct     1320

ggcggacctc tccccgattt tcctaatcac agtcttactc tgaagagaga caaggctcct     1380

aaaagttcct ttgtagggga cggtgacata ttcaagaaac tggacagtga gctctctcga     1440

gcacaggaaa aggccttgga taccagctat gtgattctgc ccacagctac cgccacactg     1500

cggccaaagc caaaagagga gccaaaatat agtatccata tagaccaaat gccacaaacc     1560

cgactgatac acttgtcaac cgcccccgaa gctagccttc ccgctaggtc accacccagt     1620

cggcaacctc cctccggtgg tcctcctgag gcccctccag cccagccccc accacctcct     1680

cctccacctc ctcctccccc ccagcagcct ttgcctcccc caccaaatct tgagcctgct     1740

ccaccaagcc tcggggaccc cggggagcct gccgcacatc ctgggccaag taccggacca     1800

tctactaaga acgagaacgt agcaacactc tctgtttcat ctctggagcg ccgcaaatca     1860

agatatgcag agcttgattt cgagaagatc atgcatacta ggaaaagaca tcaagacatg     1920

ttccaagacc tgaatcggaa gctgcagcac gccgcagaga aggataaaga ggttctcgga     1980

cccgatagta agcctgaaaa acaacaaacc cccaataaaa ggccctggga aagtcttagg     2040

aaagcccatg gtacccctac ctgggttaag aaagagctgg aaccacttca accatcacca     2100

ttggagttga ggtcagtgga gtgggaaagg tcaggggcaa ccataccctt ggtaggacaa     2160

gatatcatag acctgcagac tgaagtcggg tccggaagta tggtgagcaa gggcgaggag     2220

ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag     2280

ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc     2340

atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac     2400

ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc     2460

gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac     2520

aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag     2580

ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac     2640

agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag     2700

atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc     2760

cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag     2820

ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc     2880

gccgggatca ctctcggcat ggacgagctg tacaagtaa                            2919


