                         SEQUENCE LISTING

<110>  JUNO THERAPEUTICS, INC.
       Teoh, Jeffrey
       Cossette, Daniel
       Larson, Ryan
       Cooper, Sara
 
<120>  PROCESS FOR PRODUCING GENETICALLY ENGINEERED CELLS

<130>  735042017640

<140> Not Yet Assigned    
<141> Concurrently Herewith  

<150>  62/756,571
<151>  2018-11-06

<150>  62/798,457
<151>  2019-01-29

<160>  98    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Spacer(IgG4 hinge)

<400>  1

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  2
<211>  36
<212>  DNA
<213>  artificial sequence


<220>
<221>  misc_feature
<223>  Spacer (IgG4hinge)

<400>  2
gaatctaagt acggaccgcc ctgcccccct tgccct                                 36


<210>  3
<211>  119
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Hinge-CH3 spacer

<400>  3

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  4
<211>  229
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Hinge-CH2-CH3-spacer

<400>  4

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  5
<211>  282
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  IgD-hinge-Fc

<400>  5

Arg Trp Pro Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala 
1               5                   10                  15      


Gln Pro Gln Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala 
            20                  25                  30          


Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys 
        35                  40                  45              


Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro 
    50                  55                  60                  


Ser His Thr Gln Pro Leu Gly Val Tyr Leu Leu Thr Pro Ala Val Gln 
65                  70                  75                  80  


Asp Leu Trp Leu Arg Asp Lys Ala Thr Phe Thr Cys Phe Val Val Gly 
                85                  90                  95      


Ser Asp Leu Lys Asp Ala His Leu Thr Trp Glu Val Ala Gly Lys Val 
            100                 105                 110         


Pro Thr Gly Gly Val Glu Glu Gly Leu Leu Glu Arg His Ser Asn Gly 
        115                 120                 125             


Ser Gln Ser Gln His Ser Arg Leu Thr Leu Pro Arg Ser Leu Trp Asn 
    130                 135                 140                 


Ala Gly Thr Ser Val Thr Cys Thr Leu Asn His Pro Ser Leu Pro Pro 
145                 150                 155                 160 


Gln Arg Leu Met Ala Leu Arg Glu Pro Ala Ala Gln Ala Pro Val Lys 
                165                 170                 175     


Leu Ser Leu Asn Leu Leu Ala Ser Ser Asp Pro Pro Glu Ala Ala Ser 
            180                 185                 190         


Trp Leu Leu Cys Glu Val Ser Gly Phe Ser Pro Pro Asn Ile Leu Leu 
        195                 200                 205             


Met Trp Leu Glu Asp Gln Arg Glu Val Asn Thr Ser Gly Phe Ala Pro 
    210                 215                 220                 


Ala Arg Pro Pro Pro Gln Pro Gly Ser Thr Thr Phe Trp Ala Trp Ser 
225                 230                 235                 240 


Val Leu Arg Val Pro Ala Pro Pro Ser Pro Gln Pro Ala Thr Tyr Thr 
                245                 250                 255     


Cys Val Val Ser His Glu Asp Ser Arg Thr Leu Leu Asn Ala Ser Arg 
            260                 265                 270         


Ser Leu Glu Val Ser Tyr Val Thr Asp His 
        275                 280         


<210>  6
<211>  24
<212>  PRT
<213>  artificial sequence

<220>
<223>  T2A

<400>  6

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro Arg 
            20                  


<210>  7
<211>  357
<212>  PRT
<213>  artificial sequence

<220>
<223>  tEGFR

<400>  7

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 
            20                  25                  30          


Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 
        35                  40                  45              


Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 
    50                  55                  60                  


Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 
65                  70                  75                  80  


Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 
                85                  90                  95      


Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 
            100                 105                 110         


Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 
        115                 120                 125             


Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 
    130                 135                 140                 


Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 
145                 150                 155                 160 


Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 
                165                 170                 175     


Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 
            180                 185                 190         


Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 
        195                 200                 205             


Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 
    210                 215                 220                 


Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 
225                 230                 235                 240 


Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 
                245                 250                 255     


Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 
            260                 265                 270         


His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 
        275                 280                 285             


Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 
    290                 295                 300                 


Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 
305                 310                 315                 320 


Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala 
                325                 330                 335     


Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly 
            340                 345                 350         


Ile Gly Leu Phe Met 
        355         


<210>  8
<211>  27
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  cd28

<300>
<308>  P10747
<309>  1989-07-01
<313>  (153)..(179)

<400>  8

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  9
<211>  66
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CD28

<300>
<308>  P10747
<309>  1989-07-01
<313>  (114)..(179)

<400>  9

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
        35                  40                  45              


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    50                  55                  60                  


Trp Val 
65      


<210>  10
<211>  41
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CD28

<300>
<308>  P10747
<309>  1989-07-01
<313>  (180)..(220)

<400>  10

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  11
<211>  41
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CD28 (LL to GG)

<400>  11

Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  12
<211>  42
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  4-1BB

<300>
<308>  Q07011.1
<309>  1995-02-01
<313>  (214)..(255)

<400>  12

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  13
<211>  112
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CD3 zeta

<400>  13

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  14
<211>  112
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CD3 zeta

<400>  14

Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  15
<211>  112
<212>  PRT
<213>  homo sapiens


<220>
<221>  MISC_FEATURE
<223>  CD3 zeta

<400>  15

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  16
<211>  335
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  tEGFR

<400>  16

Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      


Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          


Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              


Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  


Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  


Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      


Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         


Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             


Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 


Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 


Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     


Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         


Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             


Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 


Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 


Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     


His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         


Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             


Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 


Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 


Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
                325                 330                 335 


<210>  17
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  T2A

<400>  17

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  18
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  P2A

<400>  18

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  19
<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  P2A

<400>  19

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  20
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  E2A

<400>  20

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  21
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  F2A

<400>  21

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  22
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker


<220>
<221>  REPEAT
<222>  (5)..(9)
<223>  SGGGG is repeated 5 times

<220>
<221>  MISC_FEATURE
<222>  (5)..(9)
<223>  wherein P is proline, G is glycine and S is serine

<400>  22

Pro Gly Gly Gly Ser Gly Gly Gly Gly Pro 
1               5                   10  


<210>  23
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  23

Gly Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Gly Lys 
1               5                   10                  15      


Ser 
    


<210>  24
<211>  66
<212>  DNA
<213>  artificial sequence

<220>
<223>  GMCSFR alpha chain signal sequence

<400>  24
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atccca                                                                  66


<210>  25
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  GMCSFR alpha chain signal sequence

<400>  25

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  26
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  CD8 alpha signal peptide

<400>  26

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala 
        


<210>  27
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  27

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  28
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  28

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  29
<211>  61
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  29

Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro 
1               5                   10                  15      


Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu 
            20                  25                  30          


Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu Pro 
        35                  40                  45              


Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
    50                  55                  60      


<210>  30
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  30

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  31
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa is Glycine, Cysteine or Arginine

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is Cysteine or Threonine

<400>  31

Xaa Pro Pro Xaa Pro 
1               5   


<210>  32
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  32

Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   


<210>  33
<211>  10
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  33

Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10  


<210>  34
<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  Hinge

<400>  34

Glu Val Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10                  


<210>  35
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR L1

<400>  35

Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn 
1               5                   10      


<210>  36
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR L2

<400>  36

Ser Arg Leu His Ser Gly Val 
1               5           


<210>  37
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR L3

<400>  37

Gly Asn Thr Leu Pro Tyr Thr Phe Gly 
1               5                   


<210>  38
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR H1

<400>  38

Asp Tyr Gly Val Ser 
1               5   


<210>  39
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR H2

<400>  39

Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
1               5                   10                  15      


<210>  40
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR H3

<400>  40

Tyr Ala Met Asp Tyr Trp Gly 
1               5           


<210>  41
<211>  120
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH

<400>  41

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  42
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL

<400>  42

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         


<210>  43
<211>  245
<212>  PRT
<213>  artificial sequence

<220>
<223>  scFv

<400>  43

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 
            100                 105                 110         


Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 
        115                 120                 125             


Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 
    130                 135                 140                 


Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 
145                 150                 155                 160 


Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
                165                 170                 175     


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 
            180                 185                 190         


Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 
        195                 200                 205             


Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 
    210                 215                 220                 


Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
225                 230                 235                 240 


Val Thr Val Ser Ser 
                245 


<210>  44
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR L1

<400>  44

Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala 
1               5                   10      


<210>  45
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR L2

<400>  45

Ser Ala Thr Tyr Arg Asn Ser 
1               5           


<210>  46
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR L3

<400>  46

Gln Gln Tyr Asn Arg Tyr Pro Tyr Thr 
1               5                   


<210>  47
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR H1

<400>  47

Ser Tyr Trp Met Asn 
1               5   


<210>  48
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR H2

<400>  48

Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  49
<211>  13
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR H3

<400>  49

Lys Thr Ile Ser Ser Val Val Asp Phe Tyr Phe Asp Tyr 
1               5                   10              


<210>  50
<211>  122
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH

<400>  50

Glu Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Gln Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Gly Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Lys Thr Ile Ser Ser Val Val Asp Phe Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120         


<210>  51
<211>  108
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL

<400>  51

Asp Ile Glu Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Val Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Pro Leu Ile 
        35                  40                  45              


Tyr Ser Ala Thr Tyr Arg Asn Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Asn Val Gln Ser 
65                  70                  75                  80  


Lys Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Arg Tyr Pro Tyr 
                85                  90                  95      


Thr Ser Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  52
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  52

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  53
<211>  245
<212>  PRT
<213>  artificial sequence

<220>
<223>  scFv

<400>  53

Glu Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Gln Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Gly Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Lys Thr Ile Ser Ser Val Val Asp Phe Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Glu Leu Thr Gln Ser 
    130                 135                 140                 


Pro Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val Ser Val Thr Cys 
145                 150                 155                 160 


Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Ser Pro Lys Pro Leu Ile Tyr Ser Ala Thr Tyr Arg Asn 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Thr Ile Thr Asn Val Gln Ser Lys Asp Leu Ala Asp Tyr Phe 
    210                 215                 220                 


Cys Gln Gln Tyr Asn Arg Tyr Pro Tyr Thr Ser Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Leu Glu Ile Lys Arg 
                245 


<210>  54
<211>  12
<212>  PRT
<213>  artificial sequence

<220>
<223>  HC-CDR3

<400>  54

His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 
1               5                   10          


<210>  55
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  LC-CDR2

<400>  55

His Thr Ser Arg Leu His Ser 
1               5           


<210>  56
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  LC-CDR3

<400>  56

Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
1               5                   


<210>  57
<211>  735
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence encoding scFv

<400>  57
gacatccaga tgacccagac cacctccagc ctgagcgcca gcctgggcga ccgggtgacc       60

atcagctgcc gggccagcca ggacatcagc aagtacctga actggtatca gcagaagccc      120

gacggcaccg tcaagctgct gatctaccac accagccggc tgcacagcgg cgtgcccagc      180

cggtttagcg gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag      240

gaagatatcg ccacctactt ttgccagcag ggcaacacac tgccctacac ctttggcggc      300

ggaacaaagc tggaaatcac cggcagcacc tccggcagcg gcaagcctgg cagcggcgag      360

ggcagcacca agggcgaggt gaagctgcag gaaagcggcc ctggcctggt ggcccccagc      420

cagagcctga gcgtgacctg caccgtgagc ggcgtgagcc tgcccgacta cggcgtgagc      480

tggatccggc agccccccag gaagggcctg gaatggctgg gcgtgatctg gggcagcgag      540

accacctact acaacagcgc cctgaagagc cggctgacca tcatcaagga caacagcaag      600

agccaggtgt tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc      660

gccaagcact actactacgg cggcagctac gccatggact actggggcca gggcaccagc      720

gtgaccgtga gcagc                                                       735


<210>  58
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  Linker

<400>  58

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  59
<211>  326
<212>  PRT
<213>  homo sapiens

<220>
<223>  Human IgG2 Fc (Uniprot P01859)

<400>  59

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  60
<211>  327
<212>  PRT
<213>  homo sapiens

<220>
<223>  Human IgG4 Fc (Uniprot P01861)

<400>  60

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  61
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  FKBP

<400>  61

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Met Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  62
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  FKBP12v36

<400>  62

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         


<210>  63
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  acylation motif

<400>  63

Met Gly Ser Asn Lys Ser Lys Pro Lys Asp Ala Ser Gln Arg Arg Arg 
1               5                   10                  15      


<210>  64
<211>  5
<212>  PRT
<213>  artificial sequence

<220>
<223>  dual acylation region

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is any amino acid

<400>  64

Met Gly Cys Xaa Cys 
1               5   


<210>  65
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  CAAX box

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is any amino acid

<400>  65

Cys Ala Ala Xaa 
1               


<210>  66
<211>  159
<212>  PRT
<213>  Streptomyces avidinii

<400>  66

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  67
<211>  126
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MISC_FEATURE
<223>  minimal streptavidin

<400>  67

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser 
            20                  25                  30          


Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  68
<211>  126
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Mutein Streptavidin Ile44-Gly45-Ala-46-Arg47

<400>  68

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Ile Gly 
            20                  25                  30          


Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  69
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Strep-tag II

<400>  69

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  70
<211>  28
<212>  PRT
<213>  artificial sequence

<220>
<223>  Twin-Strep-tag

<400>  70

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
            20                  25              


<210>  71
<211>  24
<212>  PRT
<213>  artificial sequence

<220>
<223>  Twin-Strep-tag

<400>  71

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Trp Ser His Pro Gln Phe Glu Lys 
            20                  


<210>  72
<211>  28
<212>  PRT
<213>  artificial sequence

<220>
<223>  Twin-Strep-tag

<400>  72

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25              


<210>  73
<211>  127
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Mutein Streptavidin Ile44-Gly45-Ala-46-Arg47

<400>  73

Met Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Ile 
            20                  25                  30          


Gly Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


<210>  74
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Streptavidin-binding peptide


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is any amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa is Gly or Glu

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Gly, Lys or Arg

<400>  74

Trp Xaa His Pro Gln Phe Xaa Xaa 
1               5               


<210>  75
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Streptavidin binding peptide, Strep-tag

<400>  75

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  76
<211>  17
<212>  PRT
<213>  artificial sequence

<220>
<223>  Sequential modules of streptavidin-binding peptide


<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa is any amino acid

<220>
<221>  REPEAT
<222>  (9)..(9)
<223>  Xaa is repeated 8 or 12 times

<400>  76

Trp Ser His Pro Gln Phe Glu Lys Xaa Trp Ser His Pro Gln Phe Glu 
1               5                   10                  15      


Lys 
    


<210>  77
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  Sequential modules of streptavidin-binding peptide


<220>
<221>  REPEAT
<222>  (9)..(12)
<223>  GGGS is repeated 2 or 3 times

<400>  77

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Trp Ser His Pro 
1               5                   10                  15      


Gln Phe Glu Lys 
            20  


<210>  78
<211>  30
<212>  PRT
<213>  artificial sequence

<220>
<223>  Twin-Strep-tag

<400>  78

Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30  


<210>  79
<211>  30
<212>  PRT
<213>  artificial sequence

<220>
<223>  Twin-Strep-tag

<400>  79

Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30  


<210>  80
<211>  159
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mutein Streptavidin Val44-Thr45-Ala46-Arg47

<400>  80

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr Ala Arg Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  81
<211>  126
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mutein Streptavidin Val44-Thr45-Ala46-Arg47

<400>  81

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr 
            20                  25                  30          


Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  82
<211>  127
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Mutein Streptavidin Val44-Thr45-Ala46-Arg47

<400>  82

Met Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val 
            20                  25                  30          


Thr Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


<210>  83
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  Streptavidin-binding peptide

<400>  83

His Pro Gln Phe 
1               


<210>  84
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  Streptavidin-binding peptide


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa is Trp, Lys or Arg

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is any amino acid

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa is Gly or Glu

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Gly, Lys or Arg

<400>  84

Xaa Xaa His Pro Gln Phe Xaa Xaa 
1               5               


<210>  85
<211>  159
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Mutein Streptavidin Ile44-Gly45-Ala-46-Arg47

<400>  85

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Ile Gly Ala Arg Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  86
<211>  126
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Mutein Streptavidin Ile44-Gly45-Ala-46-Arg47 and Glu117, Gly120, 
       Try121 (mutein m1-9)

<400>  86

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr 
            20                  25                  30          


Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Glu Asn Ala Gly Tyr Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  87
<211>  139
<212>  PRT
<213>  artificial sequence


<220>
<221>  MISC_FEATURE
<223>  Mutein Streptavidin Ile44-Gly45-Ala-46-Arg47 and Glu117, Gly120, 
       Try121 (mutein m1-9)

<400>  87

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr Ala Arg Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Glu Asn Ala Gly Tyr Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
    130                 135                 


<210>  88
<211>  127
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MISC_FEATURE
<223>  Minimal streptavidin

<400>  88

Met Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu 
            20                  25                  30          


Ser Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


<210>  89
<211>  24
<212>  PRT
<213>  artificial sequence

<220>
<223>  Mutein Streptavidin


<220>
<221>  REPEAT
<222>  (9)..(12)
<223>  GGGS is repeted 2 times

<400>  89

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Ser Ala 
1               5                   10                  15      


Trp Ser His Pro Gln Phe Glu Lys 
            20                  


<210>  90
<211>  9
<212>  PRT
<213>  artificial sequence

<220>
<223>  Strep-tag

<400>  90

Ala Trp Ala His Pro Gln Phe Gly Gly 
1               5                   


<210>  91
<211>  116
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH anti-CD28 antibody CD28.3

<400>  91

Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val Arg 
1               5                   10                  15      


Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Glu Tyr Ile Ile His 
            20                  25                  30          


Trp Ile Lys Leu Arg Ser Gly Gln Gly Leu Glu Trp Ile Gly Trp Phe 
        35                  40                  45              


Tyr Pro Gly Ser Asn Asp Ile Gln Tyr Asn Ala Lys Phe Lys Gly Lys 
    50                  55                  60                  


Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Val Tyr Met Glu Leu 
65                  70                  75                  80  


Thr Gly Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Arg 
                85                  90                  95      


Asp Asp Phe Ser Gly Tyr Asp Ala Leu Pro Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Met Val Thr Val 
        115     


<210>  92
<211>  108
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL anti-CD28 antibody CD28.3 

<400>  92

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Val Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys Arg Thr Asn Glu Asn Ile Tyr Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Thr His Leu Val Glu Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Thr Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe Trp Gly Thr Pro Cys 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  93
<211>  119
<212>  PRT
<213>  artificial sequence

<220>
<223>  VH anti-CD3 antibody OKT3

<400>  93

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  94
<211>  106
<212>  PRT
<213>  artificial sequence

<220>
<223>  VL anti-CD3 antibody OKT3

<400>  94

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Asn 
            100                 105     


<210>  95
<211>  248
<212>  PRT
<213>  artificial sequence

<220>
<223>  Variable Heavy chain of Fab fragment m13B8.2 

<400>  95

Ala Met Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Leu Val Gln Pro 
1               5                   10                  15      


Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 
            20                  25                  30          


Thr Phe Gly Val His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Val Ile Trp Ala Ser Gly Ile Thr Asp Tyr Asn Val Pro 
    50                  55                  60                  


Phe Met Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val 
65                  70                  75                  80  


Phe Phe Lys Leu Asn Ser Leu Gln Pro Asp Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Lys Asn Asp Pro Gly Thr Gly Phe Ala Tyr Trp Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Thr Lys Gly Pro Ser Val Phe Pro Ala Pro 
        115                 120                 125             


Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val 
    130                 135                 140                 


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
145                 150                 155                 160 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Leu 
                165                 170                 175     


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
            180                 185                 190         


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
        195                 200                 205             


Asp Lys Lys Val Glu Pro Lys Ser Cys Gly Ser Ala Trp Ser His Pro 
    210                 215                 220                 


Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Ala 
225                 230                 235                 240 


Trp Ser His Pro Gln Phe Glu Lys 
                245             


<210>  96
<211>  218
<212>  PRT
<213>  artificial sequence

<220>
<223>  Variable Light chain of Fab Fragment m13B8.2 

<400>  96

Ala Met Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser 
1               5                   10                  15      


Val Gly Glu Thr Val Thr Phe Thr Cys Arg Ala Ser Glu Met Ile Tyr 
            20                  25                  30          


Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu 
        35                  40                  45              


Leu Val His Asp Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Gly Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Asn Thr Leu 
65                  70                  75                  80  


Gln Pro Glu Asp Phe Gly Thr Tyr Tyr Cys Gln Ala His Tyr Gly Asn 
                85                  90                  95      


Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Ile 
            100                 105                 110         


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
        115                 120                 125             


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
    130                 135                 140                 


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
145                 150                 155                 160 


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
                165                 170                 175     


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
            180                 185                 190         


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
        195                 200                 205             


Lys Ser Phe Asn Arg Gly Glu Cys Gly Ser 
    210                 215             


<210>  97
<211>  118
<212>  PRT
<213>  artificial sequence

<220>
<223>  Variable Heavy chain of huOKT8 

<400>  97

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Asn Asp Asn Thr Leu Tyr Ala Ser Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Gly Tyr Tyr Val Phe Asp His Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  98
<211>  107
<212>  PRT
<213>  artificial sequence

<220>
<223>  Variable Light chain of huOKT8 

<400>  98

Asp Val Gln Ile Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Thr Ser Arg Ser Ile Ser Gln Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Gly Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln His Asn Glu Asn Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


