
                                SEQUENCE LISTING

<110> GENENTECH INC.

<120> METHODS OF PRODUCING TWO CHAIN PROTEINS IN PROKARYOTIC HOST CELLS

<130> 14639-20440.40

<140> Not Yet Assigned
<141> Concurrently Herewith

<150> US 62/755,915
<151> 2018-11-05

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 132
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 1
Met Ala Leu Leu Leu Thr Thr Val Ile Ala Leu Thr Cys Leu Gly Gly
 1               5                  10                  15      
Phe Ala Ser Pro Gly Pro Val Pro Pro Ser Thr Ala Leu Arg Glu Leu
            20                  25                  30          
Ile Glu Glu Leu Val Asn Ile Thr Gln Asn Gln Lys Ala Pro Leu Cys
        35                  40                  45              
Asn Gly Ser Met Val Trp Ser Ile Asn Leu Thr Ala Gly Met Tyr Cys
    50                  55                  60                  
Ala Ala Leu Glu Ser Leu Ile Asn Val Ser Gly Cys Ser Ala Ile Glu
65                  70                  75                  80  
Lys Thr Gln Arg Met Leu Ser Gly Phe Cys Pro His Lys Val Ser Ala
                85                  90                  95      
Gly Gln Phe Ser Ser Leu His Val Arg Asp Thr Lys Ile Glu Val Ala
            100                 105                 110         
Gln Phe Val Lys Asp Leu Leu Leu His Leu Lys Lys Leu Phe Arg Glu
        115                 120                 125             
Gly Arg Phe Asn
    130         


<210> 2
<211> 114
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 2
Ser Pro Gly Pro Val Pro Pro Ser Thr Ala Leu Arg Glu Leu Ile Glu
 1               5                  10                  15      
Glu Leu Val Asn Ile Thr Gln Asn Gln Lys Ala Pro Leu Cys Asn Gly
            20                  25                  30          
Ser Met Val Trp Ser Ile Asn Leu Thr Ala Gly Met Tyr Cys Ala Ala
        35                  40                  45              
Leu Glu Ser Leu Ile Asn Val Ser Gly Cys Ser Ala Ile Glu Lys Thr
    50                  55                  60                  
Gln Arg Met Leu Ser Gly Phe Cys Pro His Lys Val Ser Ala Gly Gln
65                  70                  75                  80  
Phe Ser Ser Leu His Val Arg Asp Thr Lys Ile Glu Val Ala Gln Phe
                85                  90                  95      
Val Lys Asp Leu Leu Leu His Leu Lys Lys Leu Phe Arg Glu Gly Arg
            100                 105                 110         
Phe Asn
        


<210> 3
<211> 118
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 3
Glu Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln
 1               5                  10                  15      
Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Ala Tyr
            20                  25                  30          
Ser Val Asn Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu Trp Leu
        35                  40                  45              
Ala Met Ile Trp Gly Asp Gly Lys Ile Val Tyr Asn Ser Ala Leu Lys
    50                  55                  60                  
Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val Val Leu
65                  70                  75                  80  
Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr Cys Ala
                85                  90                  95      
Gly Asp Gly Tyr Tyr Pro Tyr Ala Met Asp Asn Trp Gly Gln Gly Ser
            100                 105                 110         
Leu Val Thr Val Ser Ser
        115             


<210> 4
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 4
Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ser Val Ser Leu Gly
 1               5                  10                  15      
Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Lys Ser Val Asp Ser Tyr
            20                  25                  30          
Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro
        35                  40                  45              
Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser
65                  70                  75                  80  
Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asn Asn
                85                  90                  95      
Glu Asp Pro Arg Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg
            100                 105                 110         


<210> 5
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 5
Ala Tyr Ser Val Asn
 1               5  


<210> 6
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 6
Met Ile Trp Gly Asp Gly Lys Ile Val Tyr Asn Ser Ala Leu Lys Ser
 1               5                  10                  15      


<210> 7
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 7
Asp Gly Tyr Tyr Pro Tyr Ala Met Asp Asn
 1               5                  10  


<210> 8
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 8
Arg Ala Ser Lys Ser Val Asp Ser Tyr Gly Asn Ser Phe Met His
 1               5                  10                  15  


<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 9
Leu Ala Ser Asn Leu Glu Ser
 1               5          


<210> 10
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Construct

<400> 10
Gln Gln Asn Asn Glu Asp Pro Arg Thr
 1               5                  

