                         SEQUENCE LISTING

<110>  Ritter, Gerd
       Dunn, Steven
 
<120>  HUMANIZED AND VARIANT TGF-B3 SPECIFIC ANTIBODIES AND METHODS AND 
       USES THEREOF

<130>  2745-4PCT

<140>  Unassigned
<141>  2019-11-04

<150>  US 62/755,840
<151>  2018-11-05

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Mus musculus

<400>  1

Ser Ser Trp Ile His 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  2

Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  3

Arg Met Ile Thr Thr Gln Ala Ala Met Asp Tyr 
1               5                   10      


<210>  4
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  4

Lys Ala Ser Gln Ser Val Ile Asn Ala Val Ala 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  5

Tyr Ala Ser Asn Arg Tyr Thr 
1               5           


<210>  6
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  6

Gln Gln Asp Tyr Ser Ser Pro Thr 
1               5               


<210>  7
<211>  112
<212>  PRT
<213>  Mus musculus

<400>  7

Ala Leu Asp Thr Asn Tyr Cys Phe Arg Asn Leu Glu Glu Asn Cys Cys 
1               5                   10                  15      


Val Arg Pro Leu Tyr Ile Asp Phe Arg Gln Asp Leu Gly Trp Lys Trp 
            20                  25                  30          


Val His Glu Pro Lys Gly Tyr Tyr Ala Asn Phe Cys Ser Gly Pro Cys 
        35                  40                  45              


Pro Tyr Leu Arg Ser Ala Asp Thr Thr His Ser Thr Val Leu Gly Leu 
    50                  55                  60                  


Tyr Asn Thr Leu Asn Pro Glu Ala Ser Ala Ser Pro Cys Cys Val Pro 
65                  70                  75                  80  


Gln Asp Leu Glu Pro Leu Thr Ile Leu Tyr Tyr Val Gly Arg Thr Pro 
                85                  90                  95      


Lys Val Glu Gln Leu Ser Asn Met Val Val Lys Ser Cys Lys Cys Ser 
            100                 105                 110         


<210>  8
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  8

Ala Leu Asp Thr Asn Tyr Cys Phe Arg Asn Leu Glu Glu Asn Cys Cys 
1               5                   10                  15      


Val Arg Pro Leu Tyr Ile Asp Phe Arg Gln Asp Leu Gly Trp Lys Trp 
            20                  25                  30          


Val His Glu Pro Lys Gly Tyr Tyr Ala Asn Phe Cys Ser Gly Pro Cys 
        35                  40                  45              


Pro Tyr Leu Arg Ser Ala Asp Thr Thr His Ser Thr Val Leu Gly Leu 
    50                  55                  60                  


Tyr Asn Thr Leu Asn Pro Glu Ala Ser Ala Ser Pro Cys Cys Val Pro 
65                  70                  75                  80  


Gln Asp Leu Glu Pro Leu Thr Ile Leu Tyr Tyr Val Gly Arg Thr Pro 
                85                  90                  95      


Lys Val Glu Gln Leu Ser Asn Met Val Val Lys Ser Cys Lys Cys Ser 
            100                 105                 110         


<210>  9
<211>  417
<212>  DNA
<213>  Mus musculus

<400>  9
atggaatggc cttgtatctt tctcttcctc ctgtcagtaa ctgaaggtgt ccactcccag       60

gttctcctgc agcagtctgg acctgagctg gtgaagcctg gggcctcagt gaagatttcc      120

tgcaaggctt ctggctacgc attcagtagc tcctggatac actgggtgaa gcagaggcct      180

ggaaagggtc ttgagtggat tggacggatt tatccgggag atggagatac taactatact      240

gggaagttca agggcaaggc cacacttact gcagacaaat cctccagcac agcctacatg      300

caactcagca gcctgacatc tgaggactct gcggtctact tctgtgcaag aaggatgatt      360

acgactcagg cggctatgga ctactggggt caaggaacct cagtcaccgt ctcctca         417


<210>  10
<211>  120
<212>  PRT
<213>  Mus musculus

<400>  10

Gln Val Leu Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  11
<211>  381
<212>  DNA
<213>  Mus musculus

<400>  11
atgaagtcac agacccaggt cttcgtattt ctactgctct gtgtgtctgg tgctcatggg       60

agttttgtga tgacccagac tcccaaattc ctgcttgtat cagcaggaga cagggttacc      120

ataacctgca aggccagtca gagtgtgatt aatgctgtag cttggtacca acagaagcca      180

gggcagtctc ctaaactgct gatatactat gcatccaatc gctacactgg agtccctgat      240

cgcttcactg gcaatggata tgggacggat ttcactttca ccatcagcac tgtgcaggct      300

gaagacctgg cagtttattt ctgtcagcag gattatagct ctccgtacac gttcggaggg      360

gggaccaagc tggaaataaa a                                                381


<210>  12
<211>  107
<212>  PRT
<213>  Mus musculus

<400>  12

Ser Phe Val Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Asn Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  13
<211>  256
<212>  PRT
<213>  Artificial sequence

<220>
<223>  murine 1901 VL-VH grafted construct

<400>  13

Ser Phe Val Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Asn Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Arg Gln Glu Gly Gly Ser Gly Glu Gly Gly Ser Gly Glu Ser Asn Ala 
        115                 120                 125             


Ala Ala Gln Val Leu Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro 
    130                 135                 140                 


Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser 
145                 150                 155                 160 


Ser Ser Trp Ile His Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu 
                165                 170                 175     


Trp Ile Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly 
            180                 185                 190         


Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 
        195                 200                 205             


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
    210                 215                 220                 


Phe Cys Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Met Asp Tyr Trp 
225                 230                 235                 240 


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys Ala Ser 
                245                 250                 255     


<210>  14
<211>  256
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_Glv1 VL-VH grafted construct

<400>  14

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Arg Gln Glu Gly Gly Ser Gly Glu Gly Gly Ser Gly Glu Ser Asn Ala 
        115                 120                 125             


Ala Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
    130                 135                 140                 


Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser 
145                 150                 155                 160 


Ser Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
                165                 170                 175     


Trp Ile Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly 
            180                 185                 190         


Lys Phe Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr 
        195                 200                 205             


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
    210                 215                 220                 


Tyr Cys Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Met Asp Tyr Trp 
225                 230                 235                 240 


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys Ala Ser 
                245                 250                 255     


<210>  15
<211>  120
<212>  PRT
<213>  Mus musculus

<400>  15

Gln Val Leu Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  16
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_Glv1 VH

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  17
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_VH_1G10

<400>  17

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asp Tyr Ser Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Leu Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  18
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_VH_1G10m

<400>  18

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asp Tyr Ser Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Leu Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  19
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_VH_1G10m_Glv1_03(K)

<400>  19

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asp Tyr Ser Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Leu Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  20
<211>  107
<212>  PRT
<213>  Mus musculus

<400>  20

Ser Phe Val Met Thr Gln Thr Pro Lys Phe Leu Leu Val Ser Ala Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Asn Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Thr Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  21
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_Glv1

<400>  21

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  22
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_VK_Glv1_03(F)

<400>  22

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  


Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  23
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LCR1901_VK_Glv1_05(H)

<400>  23

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Ser Val Ile Asn Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Tyr Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  


Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Asp Tyr Ser Ser Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  24
<211>  327
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CH IgG4(S228P)

<400>  24

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  25
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CK  IGKC*01

<400>  25

Arg Thr Ala Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  26
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  26

Ser Ser Ser Trp Ile His 
1               5       


<210>  27
<211>  16
<212>  PRT
<213>  Mus musculus

<400>  27

Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Thr Gly Lys Phe Lys 
1               5                   10                  15      


<210>  28
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH CDR1 humanized antibody

<400>  28

Gly Tyr Thr Phe Ser Ser Ser Trp Ile His 
1               5                   10  


<210>  29
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH CDR2 humanized

<400>  29

Trp Ile Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asp Tyr Ser Glu 
1               5                   10                  15      


Lys Phe Gln 
            


<210>  30
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH CDR3 humanized

<400>  30

Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Leu 
1               5                   10      


<210>  31
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VL CDR1 humanized

<400>  31

Lys Ala Ser Gln Ser Val Ile Asn Ala Val Ala Trp Tyr 
1               5                   10              


<210>  32
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VL CDR2 humanized

<400>  32

Leu Leu Ile Tyr Tyr Ala Ser Asn Arg Tyr Thr 
1               5                   10      


<210>  33
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VL CDR3 humanized

<400>  33

Gln Gln Asp Tyr Ser Ser Pro Tyr 
1               5               


<210>  34
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH CDR2 variant humanized

<400>  34

Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asp Tyr Ser Glu Lys Phe Gln 
1               5                   10                  15      


<210>  35
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH CDR3 variant humanized

<400>  35

Arg Met Ile Thr Thr Gln Ala Ala Leu Asp Tyr 
1               5                   10      


<210>  36
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH LCR1901_VH_1G10m_02(J)

<400>  36

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Ser Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Asp Tyr Ser Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Met Ile Thr Thr Gln Ala Ala Leu Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3 humanized

<400>  37

Arg Met Ile Thr Thr Gln Ala Ala Leu 
1               5                   


<210>  38
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
ctgaaaggaa ggatatagaa tgtgc                                             25


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  39
gtcgtctttc cagacgttag                                                   20


<210>  40
<211>  33
<212>  DNA
<213>  Artificial  Sequence

<220>
<223>  Primer

<400>  40
gagggtggtt ctggcgagtc caatgcsgcs gca                                    33


<210>  41
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  41
gagggtggtt ctggcgagtc caatgccryg gca                                    33


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  42
gagggtggtt ctggcgagtc caatgccatg gca                                    33


<210>  43
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
atggaccctt ggtcgacgct gaggagacgg tgaccagggt tcc                         43


<210>  44
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
atggaccctt ggtcgacgct gaggagacgg tgaccgtggt ccc                         43


<210>  45
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
atggaccctt ggtcgacgct gaggagacrg tgaccagggt scc                         43


<210>  46
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
atggaccctt ggtcgacgct gaagagacgg tgaccattgt ccc                         43


<210>  47
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
ctggctcttg gcgcggctag ccctgcsats gct                                    33


<210>  48
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
ctggctcttg gcgcggctag ccctgcgmkc gct                                    33


<210>  49
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
ctggctcttg gcgcggctag ccctgtgcgc gct                                    33


<210>  50
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  50
accaccagat ggtgcggccg cagttcgttt gatytccacc ttgg                        44


<210>  51
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  51
accaccagat ggtgcggccg cagttcgttt gatctccagc ttgg                        44


<210>  52
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  52
accaccagat ggtgcggccg cagttcgttt gatatccact ttgg                        44


<210>  53
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  53
accaccagat ggtgcggccg cagttcgttt aatctccagt cgtg                        44


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  54
gaggatttga tattcacctg g                                                 21


<210>  55
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  55
gaattcgatc aggactgaac agag                                              24


