                         SEQUENCE LISTING

<110>  Institut des sciences et industries du vivant et de 
       l'environnement -AgroParisTech
       UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC
 
<120>  Procd biocatalytique de production de 2H-HBO et d'analogues 
       bta-substitus  partir de LGO en utilisant une cyclohexanone 
       monooxygnase

<130>  AGP11 PCT

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1632
<212>  DNA
<213>  Acetinobacter sp.

<400>  1
atgtcacaaa aaatggattt tgatgctatc gtgattggtg gtggttttgg cggactttat       60

gcagtcaaaa aattaagaga cgagctcgaa cttaaggttc aggcttttga taaagccacg      120

gatgtcgcag gtacttggta ctggaaccgt tacccaggtg cattgacgga tacagaaacc      180

cacctctact gctattcttg ggataaagaa ttactacaat cgctagaaat caagaaaaaa      240

tatgtgcaag gccctgatgt acgcaagtat ttacagcaag tggctgaaaa gcatgattta      300

aagaagagct atcaattcaa taccgcggtt caatcggctc attacaacga agcagatgcc      360

ttgtgggaag tcaccactga atatggtgat aagtacacgg cgcgtttcct catcactgct      420

ttaggcttat tgtctgcgcc taacttgcca aacatcaaag gcattaatca gtttaaaggt      480

gagctgcatc ataccagccg ctggccagat gacgtaagtt ttgaaggtaa acgtgtcggc      540

gtgattggta cgggttccac cggtgttcag gttattacgg ctgtggcacc tctggctaaa      600

cacctcactg tcttccagcg ttctgcacaa tacagcgttc caattggcaa tgatccactg      660

tctgaagaag atgttaaaaa gatcaaagac aattatgaca aaatttggga tggtgtatgg      720

aattcagccc ttgcctttgg cctgaatgaa agcacagtgc cagcaatgag cgtatcagct      780

gaagaacgca aggcagtttt tgaaaaggca tggcaaacag gtggcggttt ccgtttcatg      840

tttgaaactt tcggtgatat tgccaccaat atggaagcca atatcgaagc gcaaaatttc      900

attaagggta aaattgctga aatcgtcaaa gatccagcca ttgcacagaa gcttatgcca      960

caggatttgt atgcaaaacg tccgttgtgt gacagtggtt actacaacac ctttaaccgt     1020

gacaatgtcc gtttagaaga tgtgaaagcc aatccgattg ttgaaattac cgaaaacggt     1080

gtgaaactcg aaaatggcga tttcgttgaa ttagacatgc tgatatgtgc cacaggtttt     1140

gatgccgtcg atggcaacta tgtgcgcatg gacattcaag gtaaaaacgg cttggccatg     1200

aaagactact ggaaagaagg tccgtcgagc tatatgggtg tcaccgtaaa taactatcca     1260

aacatgttca tggtgcttgg accgaatggc ccgtttacca acctgccgcc atcaattgaa     1320

tcacaggtgg aatggatcag tgataccatt caatacacgg ttgaaaacaa tgttgaatcc     1380

attgaagcga caaaagaagc ggaagaacaa tggactcaaa cttgcgccaa tattgcggaa     1440

atgaccttat tccctaaagc gcaatcctgg atttttggtg cgaatatccc gggcaagaaa     1500

aacacggttt acttctatct cggtggttta aaagaatatc gcagtgcgct agccaactgc     1560

aaaaaccatg cctatgaagg ttttgatatt caattacaac gttcagatat caagcaacct     1620

gccaatgcct aa                                                         1632


<210>  2
<211>  543
<212>  PRT
<213>  Acetinobacter sp.

<400>  2

Met Ser Gln Lys Met Asp Phe Asp Ala Ile Val Ile Gly Gly Gly Phe 
1               5                   10                  15      


Gly Gly Leu Tyr Ala Val Lys Lys Leu Arg Asp Glu Leu Glu Leu Lys 
            20                  25                  30          


Val Gln Ala Phe Asp Lys Ala Thr Asp Val Ala Gly Thr Trp Tyr Trp 
        35                  40                  45              


Asn Arg Tyr Pro Gly Ala Leu Thr Asp Thr Glu Thr His Leu Tyr Cys 
    50                  55                  60                  


Tyr Ser Trp Asp Lys Glu Leu Leu Gln Ser Leu Glu Ile Lys Lys Lys 
65                  70                  75                  80  


Tyr Val Gln Gly Pro Asp Val Arg Lys Tyr Leu Gln Gln Val Ala Glu 
                85                  90                  95      


Lys His Asp Leu Lys Lys Ser Tyr Gln Phe Asn Thr Ala Val Gln Ser 
            100                 105                 110         


Ala His Tyr Asn Glu Ala Asp Ala Leu Trp Glu Val Thr Thr Glu Tyr 
        115                 120                 125             


Gly Asp Lys Tyr Thr Ala Arg Phe Leu Ile Thr Ala Leu Gly Leu Leu 
    130                 135                 140                 


Ser Ala Pro Asn Leu Pro Asn Ile Lys Gly Ile Asn Gln Phe Lys Gly 
145                 150                 155                 160 


Glu Leu His His Thr Ser Arg Trp Pro Asp Asp Val Ser Phe Glu Gly 
                165                 170                 175     


Lys Arg Val Gly Val Ile Gly Thr Gly Ser Thr Gly Val Gln Val Ile 
            180                 185                 190         


Thr Ala Val Ala Pro Leu Ala Lys His Leu Thr Val Phe Gln Arg Ser 
        195                 200                 205             


Ala Gln Tyr Ser Val Pro Ile Gly Asn Asp Pro Leu Ser Glu Glu Asp 
    210                 215                 220                 


Val Lys Lys Ile Lys Asp Asn Tyr Asp Lys Ile Trp Asp Gly Val Trp 
225                 230                 235                 240 


Asn Ser Ala Leu Ala Phe Gly Leu Asn Glu Ser Thr Val Pro Ala Met 
                245                 250                 255     


Ser Val Ser Ala Glu Glu Arg Lys Ala Val Phe Glu Lys Ala Trp Gln 
            260                 265                 270         


Thr Gly Gly Gly Phe Arg Phe Met Phe Glu Thr Phe Gly Asp Ile Ala 
        275                 280                 285             


Thr Asn Met Glu Ala Asn Ile Glu Ala Gln Asn Phe Ile Lys Gly Lys 
    290                 295                 300                 


Ile Ala Glu Ile Val Lys Asp Pro Ala Ile Ala Gln Lys Leu Met Pro 
305                 310                 315                 320 


Gln Asp Leu Tyr Ala Lys Arg Pro Leu Cys Asp Ser Gly Tyr Tyr Asn 
                325                 330                 335     


Thr Phe Asn Arg Asp Asn Val Arg Leu Glu Asp Val Lys Ala Asn Pro 
            340                 345                 350         


Ile Val Glu Ile Thr Glu Asn Gly Val Lys Leu Glu Asn Gly Asp Phe 
        355                 360                 365             


Val Glu Leu Asp Met Leu Ile Cys Ala Thr Gly Phe Asp Ala Val Asp 
    370                 375                 380                 


Gly Asn Tyr Val Arg Met Asp Ile Gln Gly Lys Asn Gly Leu Ala Met 
385                 390                 395                 400 


Lys Asp Tyr Trp Lys Glu Gly Pro Ser Ser Tyr Met Gly Val Thr Val 
                405                 410                 415     


Asn Asn Tyr Pro Asn Met Phe Met Val Leu Gly Pro Asn Gly Pro Phe 
            420                 425                 430         


Thr Asn Leu Pro Pro Ser Ile Glu Ser Gln Val Glu Trp Ile Ser Asp 
        435                 440                 445             


Thr Ile Gln Tyr Thr Val Glu Asn Asn Val Glu Ser Ile Glu Ala Thr 
    450                 455                 460                 


Lys Glu Ala Glu Glu Gln Trp Thr Gln Thr Cys Ala Asn Ile Ala Glu 
465                 470                 475                 480 


Met Thr Leu Phe Pro Lys Ala Gln Ser Trp Ile Phe Gly Ala Asn Ile 
                485                 490                 495     


Pro Gly Lys Lys Asn Thr Val Tyr Phe Tyr Leu Gly Gly Leu Lys Glu 
            500                 505                 510         


Tyr Arg Ser Ala Leu Ala Asn Cys Lys Asn His Ala Tyr Glu Gly Phe 
        515                 520                 525             


Asp Ile Gln Leu Gln Arg Ser Asp Ile Lys Gln Pro Ala Asn Ala 
    530                 535                 540             


<210>  3
<211>  1599
<212>  DNA
<213>  Pseudomonas aeruginosa

<400>  3
gtggtagcaa ctaaagattt cgacgccatc gtcgttggcg cagggtttgg cggtctgtat       60

atgctgaaaa aactgcgcga cgagcagggt ctcaatgttc gcgtcttcga caaggcaggt      120

gatgtgggcg gaacgtggta ctggaaccgc tatccgggcg ccctgtccga tactgaaacc      180

catgtttact gctattcgtg ggacaaagaa ctgttgcagg agatggatat caccagtcgg      240

tacactaccc agccgcaaat cctcaaatac ctcgagaaag tggcggaccg ccatgatctg      300

cgcaaggata tccagttcaa tactgggatc accgcgatgc atttcaacga aacgactaac      360

ctctgggagg tgcataccga cacgggtgag tcgtacactg ccaagtttat cgtgactgcg      420

cttggtctgc tctccgccac taatattcca aaaattaaag gcctagagac cttccagaga      480

gagtgttacc acacgggtaa ctggccccag gatgttcagt tcgagggaaa acgtgtgggc      540

gtgatcggta caggatccac aggtacccag gtgatcaccg ccattgcgcc acaggtcgag      600

catctcacag tgttccagcg ttcgccgcaa tacagcgtac cggtcggtaa cggcccggtc      660

agcagagagt atgtcgacaa catcaagaag aattacgaca aaatctggga gcaagtgaaa      720

aactccatgg tcgccttcgg cttcgaggaa agcacggtgc cggcgatgag tgtttctgat      780

gaggaacgcc aggcagtctt ccagaaggct tgggaaaatg gtggcggctt tcgtttcatg      840

tttgaaacct tctgcgacat cgccacagac gaacgtgcca acaaggccgc gcaggatttc      900

atccggtcca agattgccga aatcgtcaag gatccggaaa ctgctcgcaa gctcacgccg      960

aacgacctgt acgctaagcg ccctctgtgc gacagcggtt actacgcaac gtacaaccgc     1020

ccgaacgtga gcctgctgga cgtcaaggcc aacccgatag cagagatcac ccccaaaggg     1080

gtgaaaaccg cagatggggt cgaacatgag ttggatatgc tgatctttgc caccggcttc     1140

gatgcggtgg acggtaacta caccaagatc gatatccgag gccgtaaagg gctggctatc     1200

caagaccact ggaaggcagg cccgagcagc tacctcggag ttgcgaacgc aaattacccc     1260

aacatgttca tggtgttggg tcccaatggg cccttcacca acctgccgcc ttcaatcgag     1320

acccaggtcg agtggatcag cgacctgatc caagacgtca acactaagag tcttaagaca     1380

gtggagccca cgcccgaggc tgaggcattc tggaccaaga cttgccagga aattgcaagc     1440

acgacgcttt tcccgaaggc ggagtcgtgg atttttggtg ccaatatccc gggcaagacc     1500

aacactgtat attttttctt ggccggactc ggtgcatatc ggcaacaact cactgaagta     1560

cgaaagcaag gctaccaagg tttccagttc agtaaatga                            1599


<210>  4
<211>  532
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  4

Met Val Ala Thr Lys Asp Phe Asp Ala Ile Val Val Gly Ala Gly Phe 
1               5                   10                  15      


Gly Gly Leu Tyr Met Leu Lys Lys Leu Arg Asp Glu Gln Gly Leu Asn 
            20                  25                  30          


Val Arg Val Phe Asp Lys Ala Gly Asp Val Gly Gly Thr Trp Tyr Trp 
        35                  40                  45              


Asn Arg Tyr Pro Gly Ala Leu Ser Asp Thr Glu Thr His Val Tyr Cys 
    50                  55                  60                  


Tyr Ser Trp Asp Lys Glu Leu Leu Gln Glu Met Asp Ile Thr Ser Arg 
65                  70                  75                  80  


Tyr Thr Thr Gln Pro Gln Ile Leu Lys Tyr Leu Glu Lys Val Ala Asp 
                85                  90                  95      


Arg His Asp Leu Arg Lys Asp Ile Gln Phe Asn Thr Gly Ile Thr Ala 
            100                 105                 110         


Met His Phe Asn Glu Thr Thr Asn Leu Trp Glu Val His Thr Asp Thr 
        115                 120                 125             


Gly Glu Ser Tyr Thr Ala Lys Phe Ile Val Thr Ala Leu Gly Leu Leu 
    130                 135                 140                 


Ser Ala Thr Asn Ile Pro Lys Ile Lys Gly Leu Glu Thr Phe Gln Arg 
145                 150                 155                 160 


Glu Cys Tyr His Thr Gly Asn Trp Pro Gln Asp Val Gln Phe Glu Gly 
                165                 170                 175     


Lys Arg Val Gly Val Ile Gly Thr Gly Ser Thr Gly Thr Gln Val Ile 
            180                 185                 190         


Thr Ala Ile Ala Pro Gln Val Glu His Leu Thr Val Phe Gln Arg Ser 
        195                 200                 205             


Pro Gln Tyr Ser Val Pro Val Gly Asn Gly Pro Val Ser Arg Glu Tyr 
    210                 215                 220                 


Val Asp Asn Ile Lys Lys Asn Tyr Asp Lys Ile Trp Glu Gln Val Lys 
225                 230                 235                 240 


Asn Ser Met Val Ala Phe Gly Phe Glu Glu Ser Thr Val Pro Ala Met 
                245                 250                 255     


Ser Val Ser Asp Glu Glu Arg Gln Ala Val Phe Gln Lys Ala Trp Glu 
            260                 265                 270         


Asn Gly Gly Gly Phe Arg Phe Met Phe Glu Thr Phe Cys Asp Ile Ala 
        275                 280                 285             


Thr Asp Glu Arg Ala Asn Lys Ala Ala Gln Asp Phe Ile Arg Ser Lys 
    290                 295                 300                 


Ile Ala Glu Ile Val Lys Asp Pro Glu Thr Ala Arg Lys Leu Thr Pro 
305                 310                 315                 320 


Asn Asp Leu Tyr Ala Lys Arg Pro Leu Cys Asp Ser Gly Tyr Tyr Ala 
                325                 330                 335     


Thr Tyr Asn Arg Pro Asn Val Ser Leu Leu Asp Val Lys Ala Asn Pro 
            340                 345                 350         


Ile Ala Glu Ile Thr Pro Lys Gly Val Lys Thr Ala Asp Gly Val Glu 
        355                 360                 365             


His Glu Leu Asp Met Leu Ile Phe Ala Thr Gly Phe Asp Ala Val Asp 
    370                 375                 380                 


Gly Asn Tyr Thr Lys Ile Asp Ile Arg Gly Arg Lys Gly Leu Ala Ile 
385                 390                 395                 400 


Gln Asp His Trp Lys Ala Gly Pro Ser Ser Tyr Leu Gly Val Ala Asn 
                405                 410                 415     


Ala Asn Tyr Pro Asn Met Phe Met Val Leu Gly Pro Asn Gly Pro Phe 
            420                 425                 430         


Thr Asn Leu Pro Pro Ser Ile Glu Thr Gln Val Glu Trp Ile Ser Asp 
        435                 440                 445             


Leu Ile Gln Asp Val Asn Thr Lys Ser Leu Lys Thr Val Glu Pro Thr 
    450                 455                 460                 


Pro Glu Ala Glu Ala Phe Trp Thr Lys Thr Cys Gln Glu Ile Ala Ser 
465                 470                 475                 480 


Thr Thr Leu Phe Pro Lys Ala Glu Ser Trp Ile Phe Gly Ala Asn Ile 
                485                 490                 495     


Pro Gly Lys Thr Asn Thr Val Tyr Phe Phe Leu Ala Gly Leu Gly Ala 
            500                 505                 510         


Tyr Arg Gln Gln Leu Thr Glu Val Arg Lys Gln Gly Tyr Gln Gly Phe 
        515                 520                 525             


Gln Phe Ser Lys 
    530         


<210>  5
<211>  1225
<212>  DNA
<213>  Pichia stipitis

<400>  5
atgcccatgt cttcagtcaa aatttctcca ttgaaggatt ctgaagcatt ccagtctatc       60

aaagttggta acaacactct tcaaaccaag attgtctatc caccaactac tagatttaga      120

gctttagaag accacactcc ttctgatttg caattgcagt actatggcga cagatccact      180

ttcccaggta ctttgcttat cactgaagct acttttgtct ctcctcaagc ctctggttat      240

gaaggtgctg ctccaggtat ttggactgac aagcacgcta aagcatggaa ggttattact      300

gataaagttc atgccaacgg ttctttcgtt tcaacccagt tgattttttt gggaagggtt      360

gcagatccag ctgttatgaa gacccgtggg ttgaatccag tttctgcctc tgctacttat      420

gaaagtgatg ccgctaaaga agctgccgaa gcagttggta accctgttag agctttgact      480

acccaagaag tcaaggatct tgtttacgag gcttacacca acgctgctca gaaggccatg      540

gatgctggtt tcgactatat tgaactccat gctgctcatg gctacctttt agatcaattt      600

ttgcaaccat gcaccaatca aagaactgat gaatacggtg gatccattga gaacagagcc      660

aggttaattc ttgagttgat tgaccatttg tctaccattg tcggtgctga caagattggt      720

atcagaatct ctccatgggc tactttccaa aacatgaagg ctcacaagga cactgttcac      780

ccattgacta ctttctctta cttggtccac gaattgcaac agagagctga caagggtcaa      840

ggtattgcct acatttctgt cgttgagcct cgtgtaagtg gtaacgtcga cgtctctgaa      900

gaagaccaag ctggtgacaa cgaatttgtc tccaagatct ggaagggtgt tatcttgaag      960

gcaggtaact actcctacga tgctccagag ttcaagacat tgaaggaaga tatcgctgac     1020

aagcgtacat tagttggctt ctccagatac ttcacctcga atcctaactt ggtttggaaa     1080

ttgcgtgatg gaattgactt ggtgccatac gacagaaaca cgttctacag tgacaataac     1140

tatggttaca ataccttttc tatggattcc gaagaggttg ataaagaatt agaaatcaag     1200

agagttcctt cggccattga agctt                                           1225


<210>  6
<211>  408
<212>  PRT
<213>  Pichia stipitis

<400>  6

Met Pro Met Ser Ser Val Lys Ile Ser Pro Leu Lys Asp Ser Glu Ala 
1               5                   10                  15      


Phe Gln Ser Ile Lys Val Gly Asn Asn Thr Leu Gln Thr Lys Ile Val 
            20                  25                  30          


Tyr Pro Pro Thr Thr Arg Phe Arg Ala Leu Glu Asp His Thr Pro Ser 
        35                  40                  45              


Asp Leu Gln Leu Gln Tyr Tyr Gly Asp Arg Ser Thr Phe Pro Gly Thr 
    50                  55                  60                  


Leu Leu Ile Thr Glu Ala Thr Phe Val Ser Pro Gln Ala Ser Gly Tyr 
65                  70                  75                  80  


Glu Gly Ala Ala Pro Gly Ile Trp Thr Asp Lys His Ala Lys Ala Trp 
                85                  90                  95      


Lys Val Ile Thr Asp Lys Val His Ala Asn Gly Ser Phe Val Ser Thr 
            100                 105                 110         


Gln Leu Ile Phe Leu Gly Arg Val Ala Asp Pro Ala Val Met Lys Thr 
        115                 120                 125             


Arg Gly Leu Asn Pro Val Ser Ala Ser Ala Thr Tyr Glu Ser Asp Ala 
    130                 135                 140                 


Ala Lys Glu Ala Ala Glu Ala Val Gly Asn Pro Val Arg Ala Leu Thr 
145                 150                 155                 160 


Thr Gln Glu Val Lys Asp Leu Val Tyr Glu Ala Tyr Thr Asn Ala Ala 
                165                 170                 175     


Gln Lys Ala Met Asp Ala Gly Phe Asp Tyr Ile Glu Leu His Ala Ala 
            180                 185                 190         


His Gly Tyr Leu Leu Asp Gln Phe Leu Gln Pro Cys Thr Asn Gln Arg 
        195                 200                 205             


Thr Asp Glu Tyr Gly Gly Ser Ile Glu Asn Arg Ala Arg Leu Ile Leu 
    210                 215                 220                 


Glu Leu Ile Asp His Leu Ser Thr Ile Val Gly Ala Asp Lys Ile Gly 
225                 230                 235                 240 


Ile Arg Ile Ser Pro Trp Ala Thr Phe Gln Asn Met Lys Ala His Lys 
                245                 250                 255     


Asp Thr Val His Pro Leu Thr Thr Phe Ser Tyr Leu Val His Glu Leu 
            260                 265                 270         


Gln Gln Arg Ala Asp Lys Gly Gln Gly Ile Ala Tyr Ile Ser Val Val 
        275                 280                 285             


Glu Pro Arg Val Ser Gly Asn Val Asp Val Ser Glu Glu Asp Gln Ala 
    290                 295                 300                 


Gly Asp Asn Glu Phe Val Ser Lys Ile Trp Lys Gly Val Ile Leu Lys 
305                 310                 315                 320 


Ala Gly Asn Tyr Ser Tyr Asp Ala Pro Glu Phe Lys Thr Leu Lys Glu 
                325                 330                 335     


Asp Ile Ala Asp Lys Arg Thr Leu Val Gly Phe Ser Arg Tyr Phe Thr 
            340                 345                 350         


Ser Asn Pro Asn Leu Val Trp Lys Leu Arg Asp Gly Ile Asp Leu Val 
        355                 360                 365             


Pro Tyr Asp Arg Asn Thr Phe Tyr Ser Asp Asn Asn Tyr Gly Tyr Asn 
    370                 375                 380                 


Thr Phe Ser Met Asp Ser Glu Glu Val Asp Lys Glu Leu Glu Ile Lys 
385                 390                 395                 400 


Arg Val Pro Ser Ala Ile Glu Ala 
                405             


