                         б

<110>  ҩƷ޹˾
 
<120>  TNF/IL-17AȻṹԴʽ˫쿹弰Ʊ

<130>  LZ1805868CN01

<160>  19    

<170>  PatentIn version 3.3

<210>  1
<211>  321
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  TNFɱ

<400>  1
gacatccaga tgacccaatc cccttcctcc ctcagcgcga gcgtcggaga cagggtgaca     60

atcacctgca gggcctccca gggaatccgg aactatctcg cctggtatca gcagaagccc    120

ggcaaggccc ccaagctgct gatctacgct gccagcacac tccaatccgg cgtgccttcc    180

aggttctccg gaagcggatc cggcaccgac ttcaccctca ccatttccag cctgcagccc    240

gaagacgtcg ccacctacta ctgccagcgg tacaacaggg ccccctacac cttcggccaa    300

ggcaccaagg tcgagatcaa g                                              321


<210>  2
<211>  107
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  TNFɱ

<400>  2

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Gln Arg Tyr Asn Arg Ala Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  3
<211>  321
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  TNF㶨

<400>  3
aggaccgtgg ccgcccccag cgtgttcatc ttccctccct ccgacgagca gctgaagagc     60

ggcacagcca gcgtcgtgtg cctgctcaac aacttctacc ccagggaagc caaggtgcag    120

tggaaggtgg acaacgccct gcagtccggc aacagccagg agagcgtgac cgaacaggac    180

agcaaggaca gcacctacag cctgagctcc accctcaccc tgtccaaggc cgactacgag    240

aagcataagg tgtacgcctg cgaagtgacc catcagggcc tgtccagccc cgtgacaaag    300

tccttcaaca ggggcgaatg c                                              321


<210>  4
<211>  107
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  TNF㶨

<400>  4

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  5
<211>  363
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  TNFɱ

<400>  5
gaggtgcagc tggtggaatc cggaggagga ctggtccaac ctggcaggtc cctcaggctc     60

tcctgtgctg ccagcggctt taccttcgac gattacgcta tgcattgggt gcggcaagcc    120

cctggcaaag gactggaatg ggtgagcgcc attacctgga actccggcca catcgactac    180

gccgactccg tggagggccg gtttaccatt agccgggaca acgccaagaa ctccctgtac    240

ctgcagatga acagcctgag ggctgaggac accgctgtgt actactgcgc caaggtgagc    300

tacctgtcca cagccagcag cctggattac tggggccagg gcaccctggt gacagtcagc    360

agc                                                                  363


<210>  6
<211>  121
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  TNFɱ

<400>  6

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Val Ser Tyr Leu Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  7
<211>  990
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  TNF㶨

<400>  7
gctagcacaa aaggaccttc cgtgtttcca ctggcaccct ctagtaagag tacttcagga     60

ggaaccgcag cactgggatg tctggtgaag gactacttcc cagagcccgt caccgtgtct    120

tggaacagtg gagcactgac ctccggggtc catacatttc ctgccgtgct gcagtcatcc    180

ggtctgtata gcctgagctc tgtggtcaca gtcccaagtt catccctggg cacccagaca    240

tacatctgca acgtgaatca caaaccttcc aatactaagg tcgacaagaa agtggaaccc    300

aagtcctgcg ataagaccca cacatgccct ccctgtcctg ctcccgaact gctgggagga    360

ccctccgtct tcctgttccc ccccaagccc aaagacacac tgatgatcag caggacccct    420

gaagtgacct gcgtggtcgt ggacgtgagc cacgaggacc ccgaggtcaa gtttaactgg    480

tacgtggacg gcgtggaggt ccacaacgcc aagaccaagc ccagggagga gcagtacaac    540

agcacctaca gggtcgtgtc cgtgctgacc gtgctccacc aagattggct caacggcaag    600

gagtataagt gcaaagtcag caacaaggcc ctccccgccc ccatcgagaa aaccatcagc    660

aaggccaagg gccaaccgcg ggaacctcaa gtgtataccc tccctcccag ccgggatgag    720

ctgaccaaga accaagtctc cctcttgtgc ctggtcaagg gattctaccc ttccgacatt    780

gccgtcgaat gggagagcaa tggccagccc gagaacaact acaagacaac cccccccgtc    840

ctgcgcagcg acggatcctt cttcctgtac tccaagctca ccgtggacaa gagccggtgg    900

caacagggca acgtgttctc ctgtagcgtg atgcacgaag ccctccacaa ccactatacc    960

cagaagagcc tgagcctcag ccccggcaaa                                     990


<210>  8
<211>  330
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  TNF㶨

<400>  8

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Leu Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Arg Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  9
<211>  333
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  IL-17ɱ

<400>  9
atcatcgtca tgacccagtc ccccctgtcc ctgcctgtga cactgggcca gcctgcctcc     60

atctcctgca ggtcctccca gagcctggtg cacagcaacg gcaacaccta cctgcactgg    120

tatcaacaga ggcctggcca gagccctagg ctgctgatct acaaggtgta caaccggttc    180

tccggcgtgc ctgacaggtt ctccggcagc ggttctggca ccgacttcac cctgaagatc    240

tccagggtgg aggccgagga tgtgggcgtg tacttctgca gccagtccac ccacttccct    300

accttcggcc agggcaccag gctggagatc aag                                 333


<210>  10
<211>  111
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  IL-17ɱ

<400>  10

Ile Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Tyr Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Phe Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110     


<210>  11
<211>  360
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  IL-17ɱ

<400>  11
caggtgcagg tgcaggaatc cggccctgga ctggtgaagc cctccgagac cctgtccctg     60

acctgtaccg tgtccggcaa ctccatcacc tcctactacg cctggaactg gatcaggcag    120

cctcccggaa agggcctgga gtggatgggc tacatcacct actccggcac cacctcctac    180

aacccttccc tgaagagccg gatcaccatc agcgtggaca cctccaagaa ccagttctcc    240

ctgaaactgt cctccgtgac agccgccgat accgccgtgt actactgcgc ccgggaggag    300

tacgacgaca tctacgccgt ggactactgg ggccagggca cactggtgac cgtgtcctcc    360


<210>  12
<211>  120
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  IL-17ɱ

<400>  12

Gln Val Gln Val Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asn Ser Ile Thr Ser Tyr 
            20                  25                  30          


Tyr Ala Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Met Gly Tyr Ile Thr Tyr Ser Gly Thr Thr Ser Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Ile Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Glu Tyr Asp Asp Ile Tyr Ala Val Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  13
<211>  990
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  IL-17㶨

<400>  13
gctagcacaa aaggaccttc cgtgtttcca ctggcaccct ctagtaagag tacttcagga     60

ggaaccgcag cactgggatg tctggtgaag gactacttcc cagagcccgt caccgtgtct    120

tggaacagtg gagcactgac ctccggggtc catacatttc ctgccgtgct gcagtcatcc    180

ggtctgtata gcctgagctc tgtggtcaca gtcccaagtt catccctggg cacccagaca    240

tacatctgca acgtgaatca caaaccttcc aatactaagg tcgacaagaa agtggaaccc    300

aagtcctgcg ataagaccca cacatgcccc ccttgtcctg cccctgaact gctcggaggc    360

cctagcgtgt tcctcttccc tcccaaaccc aaggacaccc tcatgatctc caggacccct    420

gaggtgacct gcgtcgtggt ggacgtcagc cacgaggacc ccgaggtgaa gttcaactgg    480

tacgtggacg gcgtcgaggt ccacaacgcc aagacaaagc ccagggagga acagtacaac    540

agcacctaca gggtggtcag cgtgctgacc gtgctgcacc aggattggct caacggcaag    600

gagtacaagt gcaaagtctc caacaaggcc ctgcccgccc ccatcgagaa gaccatctcc    660

aaggctaagg gacagcccag ggagccccaa gtgtacaccg agcctcccag ccgggatgag    720

ctgaccaaga accaagtctc cctcacctgc ctggtcaagg gattctaccc ttccgacatt    780

gccgtcgaat gggagagcaa tggccagccc gagaacaact acaagacaac cccccccgtc    840

ctggatagcg acggatcctt cttcctgctc tccgtgctca ccgtcgacaa gagcagatgg    900

cagcagggca acgtgttcag ctgtagcgtg atgcacgagg ccctgcacaa ccactacacc    960

cagaagagcc tgtccctcag ccccggcaag                                     990


<210>  14
<211>  330
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  IL-17㶨

<400>  14

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Glu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Leu Ser Val Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  15
<211>  321
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  СIL-17Aɱ

<400>  15
gacatccaga tgacacagtc ccccagcttc ctgagcgcta gcgtgggaga gagggtgacc     60

ctgagctgca aggccagcca gaacatcaac aagtacctgg actggttcca gcagaagctg    120

ggcgaagccc ccaagctgct gatctacaac gccgacaacc tccataccgg cattcccagc    180

aggttcagcg gctccggctc cttcagcgac ttcatcctga ccatcagcag cctgcagccc    240

gaggatgacg ccacctactt ctgcctgcag agggaatcct ggccttacac cttcggcgcc    300

ggcaccaagc tggaactgaa g                                              321


<210>  16
<211>  107
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  СIL-17Aɱ

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Ser Phe Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Glu Arg Val Thr Leu Ser Cys Lys Ala Ser Gln Asn Ile Asn Lys Tyr 
            20                  25                  30          


Leu Asp Trp Phe Gln Gln Lys Leu Gly Glu Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Asn Ala Asp Asn Leu His Thr Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Phe Ser Asp Phe Ile Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Asp Ala Thr Tyr Phe Cys Leu Gln Arg Glu Ser Trp Pro Tyr 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  17
<211>  366
<212>  DNA
<213>  ˹Уartificial

<220>
<223>  СIL-17Aɱ

<400>  17
caggtgcaac tgaaggagag cggccccgga ctggtgcaac cttcccaaac actcagcctc     60

acctgtaccg tctccggatt ctccctgacc aactattacg tccactgggt cagacagcct    120

cccggcaagg gactggagtg gatgggaggc gtgtggaatg acggcgacac ctcctacaac    180

agcgtcctca ggagcagact gagcattacc agagacacca gcaagagcca ggtcctcctc    240

aagatgtcct ccctccagac agaagacacc gccacctatt actgcgccag ggaaggcagg    300

gaaggcttcg tcggctacta tgtgatggat gcttggggcc ctggagctag cgtgacagtg    360

agctcc                                                               366


<210>  18
<211>  122
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  СIL-17Aɱ

<400>  18

Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Gln Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Asn Tyr 
            20                  25                  30          


Tyr Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Val Trp Asn Asp Gly Asp Thr Ser Tyr Asn Ser Val Leu Arg 
    50                  55                  60                  


Ser Arg Leu Ser Ile Thr Arg Asp Thr Ser Lys Ser Gln Val Leu Leu 
65                  70                  75                  80  


Lys Met Ser Ser Leu Gln Thr Glu Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Glu Gly Arg Glu Gly Phe Val Gly Tyr Tyr Val Met Asp Ala Trp 
            100                 105                 110         


Gly Pro Gly Ala Ser Val Thr Val Ser Ser 
        115                 120         


<210>  19
<211>  15
<212>  PRT
<213>  ˹Уartificial

<220>
<223>  GPI325-339

<400>  19

Ile Trp Tyr Ile Asn Cys Phe Gly Cys Glu Thr His Ala Met Leu 
1               5                   10                  15  



