                         SEQUENCE LISTING

<110>  Boehringer Ingelheim Vetmedica GMBH 
       Boehringer Ingelheim (China) Investment Co., Ltd.
 
<120>  Immunogenic Composition Against Avian Influenza Virus H5 Subtype

<130>  P19-0002-WO-2

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of the HA protein of strain 
       duck/China/E319-2/03 but lacking the signal peptide of the amino 
       terminus.

<400>  1

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Ser Ser Ser Phe Phe 
    130                 135                 140                 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asp Ala Tyr Pro Thr Ile 
145                 150                 155                 160 


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
                165                 170                 175     


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
            180                 185                 190         


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
        195                 200                 205             


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
    210                 215                 220                 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
225                 230                 235                 240 


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
                245                 250                 255     


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Val Glu Tyr Gly 
            260                 265                 270         


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
        275                 280                 285             


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
    290                 295                 300                 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
305                 310                 315                 320 


Pro Gln Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala 
                325                 330                 335     


Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly 
            340                 345                 350         


Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu 
        355                 360                 365             


Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile 
    370                 375                 380                 


Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn 
385                 390                 395                 400 


Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly 
                405                 410                 415     


Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu 
            420                 425                 430         


Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr 
        435                 440                 445             


Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn 
    450                 455                 460                 


Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser 
465                 470                 475                 480 


Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg 
                485                 490                 495     


Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr 
            500                 505                 510         


Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu 
        515                 520                 525             


Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser 
    530                 535                 540                 


Leu Gln Cys Arg Ile Cys Ile 
545                 550     


<210>  2
<211>  552
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of H5Con1 (without signal peptide)

<400>  2

Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
1               5                   10                  15      


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
            20                  25                  30          


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
        35                  40                  45              


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
    50                  55                  60                  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
                85                  90                  95      


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
            100                 105                 110         


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
        115                 120                 125             


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Lys Ser Ser Phe Phe 
    130                 135                 140                 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Ala Tyr Pro Thr Ile 
145                 150                 155                 160 


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
                165                 170                 175     


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln 
            180                 185                 190         


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
        195                 200                 205             


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
    210                 215                 220                 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
225                 230                 235                 240 


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
                245                 250                 255     


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
            260                 265                 270         


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
        275                 280                 285             


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
    290                 295                 300                 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
305                 310                 315                 320 


Pro Gln Arg Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
                325                 330                 335     


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
            340                 345                 350         


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
        355                 360                 365             


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
    370                 375                 380                 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
385                 390                 395                 400 


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
                405                 410                 415     


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
            420                 425                 430         


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
        435                 440                 445             


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
    450                 455                 460                 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
465                 470                 475                 480 


Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
                485                 490                 495     


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
            500                 505                 510         


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
        515                 520                 525             


Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
    530                 535                 540                 


Ser Leu Gln Cys Arg Ile Cys Ile 
545                 550         


<210>  3
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of H5Con3

<400>  3

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Leu Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Asn Pro Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile 
                165                 170                 175     


Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Glu Ala Glu Gln Thr Lys Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Val Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Arg Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
            500                 505                 510         


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
        515                 520                 525             


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
    530                 535                 540                 


Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
545                 550                 555                 560 


Ser Leu Gln Cys Arg Ile Cys Ile 
                565             


<210>  4
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of H5Con5

<400>  4

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Asn Pro Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Val Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly 
        355                 360                 365             


Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu 
    370                 375                 380                 


Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile 
385                 390                 395                 400 


Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn 
                405                 410                 415     


Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly 
            420                 425                 430         


Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu 
        435                 440                 445             


Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr 
    450                 455                 460                 


Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn 
465                 470                 475                 480 


Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser 
                485                 490                 495     


Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg 
            500                 505                 510         


Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr 
        515                 520                 525             


Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu 
    530                 535                 540                 


Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser 
545                 550                 555                 560 


Leu Gln Cys Arg Ile Cys Ile 
                565         


<210>  5
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of Con5Mut

<400>  5

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Asn Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly 
225                 230                 235                 240 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Val Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Arg Glu Arg Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
            500                 505                 510         


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly 
        515                 520                 525             


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
    530                 535                 540                 


Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
545                 550                 555                 560 


Ser Leu Gln Cys Arg Ile Cys Ile 
                565             


<210>  6
<211>  241
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence from position 60 to 300 of H5Con5Mut

<400>  6

Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly 
1               5                   10                  15      


Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu 
            20                  25                  30          


Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr 
        35                  40                  45              


Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg 
    50                  55                  60                  


Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Asn Ser Trp Ser 
65                  70                  75                  80  


Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly 
                85                  90                  95      


Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn 
            100                 105                 110         


Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp 
        115                 120                 125             


Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala Ala Glu Gln 
    130                 135                 140                 


Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser 
145                 150                 155                 160 


Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val 
                165                 170                 175     


Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro 
            180                 185                 190         


Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu 
        195                 200                 205             


Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser 
    210                 215                 220                 


Glu Val Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly 
225                 230                 235                 240 


Ala 
    


<210>  7
<211>  1707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized nucleic acid sequences of H5Con1

<400>  7
atggaaaaaa tcgtgctgct cttggctatt gtcagtttgg ttaagtcgga ccagatctgc       60

attggatacc acgccaacaa ctctaccgaa caagtcgaca ctatcatgga gaagaacgtt      120

acagtgacgc acgctcagga tatcctggag aagacccata acggaaaact gtgtgacctc      180

gatggtgtga agcctttgat cctgcgtgac tgctctgtgg ctggttggct gctgggaaac      240

cctatgtgtg atgagttcat caacgtcccc gaatggtctt acattgttga gaaggctaac      300

ccagccaacg acctctgcta cccgggtaac ttcaacgatt acgaggaatt gaagcacttg      360

ctgagtagga tcaaccattt cgaaaagatc cagatcatcc caaagtccag ctggtccgac      420

cacgaggctt cttcaggagt gagttcggcc tgtccgtacc agggcaagtc cagcttcttc      480

cgtaacgtgg tctggctgat taagaaaaac aacgcctacc caacaatcaa gaggtcatac      540

aacaacacga accaggaaga cctcttggtg ctgtggggta tccaccatcc aaacgatgct      600

gccgagcaga ctcgtctcta ccaaaacccg accacttaca tctccgtcgg caccagcact      660

ttgaaccaga gactggttcc gaagatcgca acacgctcca aagtgaacgg tcaaagcggc      720

aggatggact tcttctggac gatcctgaag cctaacgatg ctattaactt cgaatccaac      780

ggaaacttca tcgcacccga gtacgcgtac aagattgtca agaaaggtga ctctgcaatc      840

atgaaatcag agctggaata cggtaactgc aacacaaagt gtcaaacgcc catgggcgcg      900

atcaactctt caatgccttt ccacaacatc catcccctca caattggcga gtgccctaag      960

tacgttaaaa gtaacagact cgtgttggct accggattgc gtaactcgcc ccagagggaa     1020

cgccgtagga agaaacgcgg actgttcggt gctatcgccg gtttcattga gggtggctgg     1080

caaggcatgg tggacggctg gtacggatac caccatagta acgaacaggg atcgggttac     1140

gcagcggaca aggagtctac ccaaaaagct atcgatggtg tgactaacaa ggtcaactca     1200

atcattgaca aaatgaacac tcagttcgag gccgtcggca gagaattcaa caacctcgag     1260

agacgcatcg aaaacttgaa caagaaaatg gaagacggat tcctggatgt ctggacctac     1320

aacgctgagc tgctcgttct gatggagaac gaacgcactc tcgacttcca cgattccaac     1380

gtgaagaacc tctacgacaa agtcagactg caactccgcg ataacgctaa ggaattgggc     1440

aacggatgct tcgagttcta ccataagtgc gacaacgagt gtatggaatc cgtccgcaac     1500

ggcacatacg attacccaca gtacagcgag gaagctcgtc tcaagaggga ggaaatttct     1560

ggcgttaaat tggagtcaat cggaacatac caaatcctgt ccatttacag cacggttgca     1620

agttcgttgg cactggcgat catggtggcg ggcctcagct tgtggatgtg ctctaacgga     1680

tcactgcagt gccgcatctg tatttaa                                         1707


<210>  8
<211>  1707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized nucleic acid sequences of H5Con3

<400>  8
atggaaaaga tcgtgctgct cttggcaatt gtctccttgg ttaagagcga ccagatctgc       60

attggatacc acgcgaacaa cagcacagaa caagtcgaca cgatcatgga gaagaacgtt      120

acagtgacgc acgcacagga tatcctggag aaaacccata acggaaagct gtgtgacctc      180

gatggtgtga agcctttgat cctgcgcgac tgctctgtcg ctggttggct gctcggcaac      240

ccaatgtgtg atgagttcat caacgtcccg gaatggtcat acattgttga gaaggcaaac      300

cctgcgaacg acctctgcta ccccggtaac ttcaacgatt acgaggaatt gaagcacttg      360

ctgtctcgta tcaaccattt cgaaaagatc cagatcatcc caaagtccag ctggagtgac      420

cacgaggcat cgctgggagt gtcttcagcg tgtccttacc aaggtaaccc cagtttcttc      480

aggaacgtgg tctggctcat taagaaaaac aacacatacc cgacgatcaa gaaatcctac      540

aacaacacta accaggaaga cctcttggtg ctgtggggta tccaccatcc taacgatgag      600

gctgaacaga caaagctcta ccaaaacccc accacttaca tctctgtcgg cacctcaact      660

ttgaaccaga gactggttcc caaaatcgct acacgcagta aggtgaacgg tcaatcgggc      720

cgtatggact tcttctggac gatcctgaag ccaaacgatg ccattaactt cgaatccaac      780

ggaaacttca tcgctccgga gtacgcctac aaaattgtca agaaaggtga ctccgctatc      840

atgaagagcg aggttgaata cggtaactgc aacaccaaat gtcaaactcc aatcggcgcc      900

attaacagtt cgatgccatt ccacaacatc catccgctga caattggcga gtgccctaag      960

tacgttaaat ctaacaagct cgtgttggct accggattgc gtaactcacc ccagagggaa     1020

cgccgtagga agaaacgcgg actgttcggt gcaatcgcgg gtttcattga gggtggctgg     1080

caaggcatgg tggacggctg gtacggatac caccattcta acgaacaggg atcaggttac     1140

gctgccgaca aagagtccac ccaaaaggca atcgatggtg tgactaacaa agtcaacagc     1200

atcattgaca agatgaacac ccagttcgag gcggtcggca gagaattcaa caacctcgag     1260

agacgcatcg aaaacttgaa caagaaaatg gaagacggat tcctggatgt ctggacctac     1320

aacgctgagc tgctcgttct gatggagaac gaacgcactc tcgacttcca cgattcaaac     1380

gtgaaaaacc tctacgacaa ggtcagactg caactccgcg ataacgccaa ggaattgggc     1440

aacggatgct tcgagttcta ccataagtgc gacaacgagt gtatggaaag tgtgcgtaac     1500

ggcacttacg attaccccca gtactcggag gaagcccgtc tcaaaaggga ggaaatttcc     1560

ggcgtcaagt tggagagcat cggaacatac caaatcctgt ctatttactc aacggttgct     1620

tccagcttgg ctctggccat catggtggcc ggcctctcct tgtggatgtg cagtaacgga     1680

tcgctgcagt gcaggatctg tatttaa                                         1707


<210>  9
<211>  1707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized nucleic acid sequences of H5Con5

<400>  9
atggaaaaga tcgtgctgct cttggctatt gtctctctgg ttaaatcaga ccagatctgc       60

attggatacc acgccaacaa ctccacagaa caagtcgaca cgatcatgga gaagaacgtt      120

acagtgacgc acgctcagga tatcctcgag aaaacccata acggaaagct gtgtgacctc      180

gatggtgtga agcctttgat cctgagggac tgcagtgtcg ccggttggct gctcggcaac      240

ccaatgtgtg atgagttcat caacgtcccg gaatggtcct acattgttga gaaggctaac      300

cctgccaacg acctctgcta ccccggtaac ttcaacgatt acgaggaatt gaagcacttg      360

ctgtctagaa tcaaccattt cgaaaagatc cagatcatcc caaagtccag ctggtcagac      420

cacgaggctt cttcaggagt gagttcggcc tgtccttacc aaggtactcc cagcttcttc      480

aggaacgtgg tctggctgat taagaaaaac aacacatacc cgacgatcaa gagatcttac      540

aacaacacta accaggaaga cctcttggtg ctgtggggta tccaccatcc taacgatgct      600

gccgagcaga caaagctcta ccaaaacccc accacttaca tcagtgtcgg cacctcgact      660

ttgaaccagc gcctggttcc caaaatcgca acacgtagta aggtgaacgg tcaatcgggc      720

aggatggact tcttctggac gatcctcaag ccaaacgatg ctattaactt cgaaagcaac      780

ggaaacttca tcgcaccgga gtacgcgtac aaaattgtca agaaaggtga ctccgcaatc      840

atgaagagcg aggttgaata cggtaactgc aacaccaaat gtcaaactcc aatcggcgcg      900

attaactcca gcatgccatt ccacaacatc catccgctga caattggcga gtgccctaag      960

tacgttaaat ctaacaagct cgtgttggcc accggattga ggaactcacc ccagagagaa     1020

cgccgtagga gaaagcgcgg actgttcggt gctatcgccg gtttcattga gggtggctgg     1080

caaggcatgg tggacggctg gtacggatac caccattcta acgaacaggg atcaggttac     1140

gcagcggaca aagagtccac ccaaaaggct atcgatggtg tgactaacaa agtcaacagc     1200

atcattgaca agatgaacac ccagttcgag gccgtcggcc gcgaattcaa caacctcgag     1260

cgccgtatcg aaaacttgaa caagaaaatg gaagacggat tcctggatgt ctggacctac     1320

aacgctgagc tgctcgttct gatggagaac gaacgtactc tcgacttcca cgattcaaac     1380

gtgaaaaacc tctacgacaa ggtccgcctg caactccgtg ataacgctaa ggaattgggc     1440

aacggatgct tcgagttcta ccataagtgc gacaacgagt gtatggaaag tgtgcgcaac     1500

ggcacttacg attaccccca gtactcggag gaagctaggt tgaaaagaga ggaaatttcc     1560

ggcgtcaagt tggagagcat cggaacatac caaatcctga gtatttactc gacggttgca     1620

tcttcattgg cactggcgat catggtggcg ggcctctcct tgtggatgtg ctccaacgga     1680

agcctgcagt gccgtatctg tatttaa                                         1707


<210>  10
<211>  1704
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized nucleic acid sequence of H5ConMut

<400>  10
atggaaaaga tcgtgctgct cttggctatt gtctctctgg ttaaatcaga ccagatctgc       60

attggatacc acgccaacaa ctccacagaa caagtcgaca cgatcatgga gaagaacgtt      120

acagtgacgc acgctcagga tatcctcgag aaaacccata acggaaagct gtgtgacctc      180

gatggtgtga agcctttgat cctgagggac tgcagtgtcg ccggttggct gctcggcaac      240

ccaatgtgtg atgagttcat caacgtcccg gaatggtcct acattgttga gaaggctaac      300

cctgccaacg acctctgcta ccccggtaac ttcaacgatt acgaggaatt gaagcacttg      360

ctgtctagaa tcaaccattt cgaaaagatc cagatcatcc caaagaatag ctggtcagac      420

cacgaggctt cttcaggagt gagttcggcc tgtccttacc aaggtactcc cagcttcttc      480

aggaacgtgg tctggctgat taagaaaaac aacacatacc cgacgatcaa gagatcttac      540

aacaacacta accaggaaga cctcttggtg ctgtggggta tccaccatcc taacgatgct      600

gccgagcaga caaagctcta ccaaaacccc accacttaca tcagtgtcgg cacctcgact      660

ttgaaccagc gcctggttcc caaaatcgca acacgtagta aggtgaacgg tcaaaatggc      720

aggatggact tcttctggac gatcctcaag ccaaacgatg ctattaactt cgaaagcaac      780

ggaaacttca tcgcaccgga gtacgcgtac aaaattgtca agaaaggtga ctccgcaatc      840

atgaagagcg aggttgaata cggtaactgc aacaccaaat gtcaaactcc aatcggcgcg      900

attaactcca gcatgccatt ccacaacatc catccgctga caattggcga gtgccctaag      960

tacgttaaat ctaacaagct cgtgttggcc accggattga ggaactcacc ccagagagaa     1020

cgccgtagga gaaagcgcgg actgttcggt gctatcgccg gtttcattga gggtggctgg     1080

caaggcatgg tggacggctg gtacggatac caccattcta acgaacaggg atcaggttac     1140

gcagcggaca aagagtccac ccaaaaggct atcgatggtg tgactaacaa agtcaacagc     1200

atcattgaca agatgaacac ccagttcgag gccgtcggcc gcgaattcaa caacctcgag     1260

cgccgtatcg aaaacttgaa caagaaaatg gaagacggat tcctggatgt ctggacctac     1320

aacgctgagc tgctcgttct gatggagaac gaacgtactc tcgacttcca cgattcaaac     1380

gtgaaaaacc tctacgacaa ggtccgcctg caactccgtg ataacgctaa ggaattgggc     1440

aacggatgct tcgagttcta ccataagtgc gacaacgagt gtatggaaag tgtgcgcaac     1500

ggcacttacg attaccccca gtactcggag gaagctaggt tgaaaagaga ggaaatttcc     1560

ggcgtcaagt tggagagcat cggaacatac caaatcctga gtatttactc gacggttgca     1620

tcttcattgg cactggcgat catggtggcg ggcctctcct tgtggatgtg ctccaacgga     1680

agcctgcagt gccgtatctg tatt                                            1704


<210>  11
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Optimized nucleic acid sequence encoding the amino acid sequence 
       from position 60 to 300 of H5Con5Mut

<400>  11
ctcgatggtg tgaagccttt gatcctgagg gactgcagtg tcgccggttg gctgctcggc       60

aacccaatgt gtgatgagtt catcaacgtc ccggaatggt cctacattgt tgagaaggct      120

aaccctgcca acgacctctg ctaccccggt aacttcaacg attacgagga attgaagcac      180

ttgctgtcta gaatcaacca tttcgaaaag atccagatca tcccaaagaa tagctggtca      240

gaccacgagg cttcttcagg agtgagttcg gcctgtcctt accaaggtac tcccagcttc      300

ttcaggaacg tggtctggct gattaagaaa aacaacacat acccgacgat caagagatct      360

tacaacaaca ctaaccagga agacctcttg gtgctgtggg gtatccacca tcctaacgat      420

gctgccgagc agacaaagct ctaccaaaac cccaccactt acatcagtgt cggcacctcg      480

actttgaacc agcgcctggt tcccaaaatc gcaacacgta gtaaggtgaa cggtcaaaat      540

ggcaggatgg acttcttctg gacgatcctc aagccaaacg atgctattaa cttcgaaagc      600

aacggaaact tcatcgcacc ggagtacgcg tacaaaattg tcaagaaagg tgactccgca      660

atcatgaaga gcgaggttga atacggtaac tgcaacacca aatgtcaaac tccaatcggc      720

gcg                                                                    723


