                               SEQUENCE LISTING

<110> SYNTHEGO CORPORATION
 
<120> METHODS FOR KNOCK-OUT OF A TARGET SEQUENCE THROUGH INTRODUCTION 
      OF A PREMATURE STOP CODON

<130> 54108-719.601

<140> PCT/US2019/059557
<141> 2019-11-01

<150> 62/754,451
<151> 2018-11-01

<150> 62/754,382
<151> 2018-11-01

<160> 63    

<170> PatentIn version 3.5

<210> 1
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 1
tgattgatca atga                                                         14


<210> 2
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
tgactgatca ctga                                                         14


<210> 3
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
tagttagtca atag                                                         14


<210> 4
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
tagctagtca ctag                                                         14


<210> 5
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
taattaatca ataa                                                         14


<210> 6
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
taactaatca ctga                                                         14


<210> 7
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
tgattgatca atag                                                         14


<210> 8
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
tgattagtca atga                                                         14


<210> 9
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
tagttgatca atga                                                         14


<210> 10
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
tagttagtca atga                                                         14


<210> 11
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
tagttgatca atag                                                         14


<210> 12
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
tgattagtca atag                                                         14


<210> 13
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
tgactgatca ctag                                                         14


<210> 14
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
tgactagtca ctga                                                         14


<210> 15
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
tagctgatca ctga                                                         14


<210> 16
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
tagctagtca ctga                                                         14


<210> 17
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
tagctgatca ctag                                                         14


<210> 18
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
tgactagtca ctag                                                         14


<210> 19
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
gucaggguuc uggauaucug                                                   20


<210> 20
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
gcugguacac ggcaggguca                                                   20


<210> 21
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
cucucagcug guacacggca                                                   20


<210> 22
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
ucucucagcu gguacacggc                                                   20


<210> 23
<211> 19
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 23
ucaggguucu ggauaucug                                                    19


<210> 24
<211> 74
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 24
cagatatcca gaaccctgac cctgcgtgta ccagctgaga gactctaaat ccagtgacaa       60

gtctgtctgc ctat                                                         74


<210> 25
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
tcaggtttcc ttgagtggca gg                                                22


<210> 26
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
taaggccgag accaccaatc ag                                                22


<210> 27
<211> 73
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 27
cagatatcca gaaccctgac cctggtgtac cagctgagag actctaaatc cagtgacaag       60

tctgtctgcc tat                                                          73


<210> 28
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (4)..(4)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (8)..(11)
<223> a, c, t, g, unknown or other

<400> 28
tgantgannn ntga                                                         14


<210> 29
<211> 65
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 29
cagatatcca gaaccctgac cctgcctgag agactctaaa tccagtgaca agtctgtctg       60

cctat                                                                   65


<210> 30
<211> 61
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 30
cagatatcca gaaccctgac gctgagagac tctaaatcca gtgacaagtc tgtctgccta       60

t                                                                       61


<210> 31
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 31
cagatatcca gaaccctgac cagctgagag actctaaatc cagtgacaag tctgtctgcc       60

tat                                                                     63


<210> 32
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 32
cagatatcca gaaccctgac cctgcgagag actctaaatc cagtgacaag tctgtctgcc       60

tat                                                                     63


<210> 33
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 33
cagatatcca gatgagagac tctaaatcca gtgacaagtc tgtctgccta t                51


<210> 34
<211> 64
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 34
cagatatcca gaaccctgac cctgctgaga gactctaaat ccagtgacaa gtctgtctgc       60

ctat                                                                    64


<210> 35
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 35
cagatatcca gaaccctgtg agagactcta aatccagtga caagtctgtc tgcctat          57


<210> 36
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(27)
<223> a, c, t, g, unknown or other

<400> 36
cagatatcca gaaccctgac cctgcnncgt gtaccagctg agagactcta aatccagtga       60

caagtctgtc tgcct                                                        75


<210> 37
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 37
cagatatcca gctgagagac tctaaatcca gtgacaagtc tgtctgccta t                51


<210> 38
<211> 64
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 38
cagatatcca gaaccctgta ccagctgaga gactctaaat ccagtgacaa gtctgtctgc       60

ctat                                                                    64


<210> 39
<211> 73
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 39
cagatatcca gaaccctgac cctgctgtac cagctgagag actctaaatc cagtgacaag       60

tctgtctgcc tat                                                          73


<210> 40
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 40
cagatatcca gaaccctgac cctgcgtacc agctgagaga ctctaaatcc agtgacaagt       60

ctgtctgcct at                                                           72


<210> 41
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(28)
<223> a, c, t, g, unknown or other

<400> 41
cagatatcca gaaccctgac cctgcnnncg tgtaccagct gagagactct aaatccagtg       60

acaagtctgt ctgcc                                                        75


<210> 42
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 42
cagatatcca gaacgctgag agactctaaa tccagtgaca agtctgtctg cctat            55


<210> 43
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 43
cagatatcca gaaccctgac agagactcta aatccagtga caagtctgtc tgcctat          57


<210> 44
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 44
cagatatcca ggtaccagct gagagactct aaatccagtg acaagtctgt ctgcctat         58


<210> 45
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 45
cagatatcca gccagctgag agactctaaa tccagtgaca agtctgtctg cctat            55


<210> 46
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 46
cagatatcca gaaccctctg agagactcta aatccagtga caagtctgtc tgcctat          57


<210> 47
<211> 68
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 47
cagatatcca gaaccctgac tgtaccagct gagagactct aaatccagtg acaagtctgt       60

ctgcctat                                                                68


<210> 48
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 48
cagatatcca gaaccctgac ccagactcta aatccagtga caagtctgtc tgcctat          57


<210> 49
<211> 164
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      MAST3 sequence

<400> 49
ccagtcaagc tcatcctccc gggaacgtct ccaccagctt cccttccagc cgacgccgga       60

cgagctgcac ttcctgtcca agcacttccg cagctcagag aatgtgcttg atgaggaagg      120

cggccggtca ccccgcctcc gaccccgctc tcgcagtctc aggt                       164


<210> 50
<211> 164
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      MAST3 sequence

<400> 50
acctgagact gcgagagcgg ggtcggaggc ggggtgaccg gccgccttcc tcatcaagca       60

cattctctga gctgcggaag tgcttggaca ggaagtgcag ctcgtccggc gtcggctgga      120

agggaagctg gtggagacgt tcccgggagg atgagcttga ctgg                       164


<210> 51
<211> 155
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 51
gtcaagctca tcctcccggg aacgtctcca ccagcttccc ttccagccga cgtgattgat       60

caatgaccgg acgagctgca cttcctgtcc aagcacttcc gcagctcaga gaatgtgctt      120

gatgaggaag gcggccggtc accccgcctc cgacc                                 155


<210> 52
<211> 155
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 52
ggtcggaggc ggggtgaccg gccgccttcc tcatcaagca cattctctga gctgcggaag       60

tgcttggaca ggaagtgcag ctcgtccggt cattgatcaa tcacgtcggc tggaagggaa      120

gctggtggag acgttcccgg gaggatgagc ttgac                                 155


<210> 53
<211> 53
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      MAST3 sequence

<400> 53
Ser Ser Ser Ser Ser Arg Glu Arg Leu His Gln Leu Pro Phe Gln Pro 
1               5                   10                  15      


Thr Pro Asp Glu Leu His Phe Leu Ser Lys His Phe Arg Ser Ser Glu 
            20                  25                  30          


Asn Val Leu Asp Glu Glu Gly Gly Arg Ser Pro Arg Leu Arg Pro Arg 
        35                  40                  45              


Ser Arg Ser Leu Ser 
    50              


<210> 54
<211> 17
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 54
Ser Ser Ser Ser Ser Arg Glu Arg Leu His Gln Leu Pro Phe Gln Pro 
1               5                   10                  15      


Thr 
    


<210> 55
<211> 91
<212> DNA
<213> Homo sapiens

<400> 55
tcctcttgtc ccacagatat ccagaaccct gaccctgccg tgtaccagct gagagactct       60

aaatccagtg acaagtctgt ctgcctattc a                                      91


<210> 56
<211> 91
<212> DNA
<213> Homo sapiens

<400> 56
tgaataggca gacagacttg tcactggatt tagagtctct cagctggtac acggcagggt       60

cagggttctg gatatctgtg ggacaagagg a                                      91


<210> 57
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 57
ctctcagctg gtacacggca                                                   20


<210> 58
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(26)
<223> a, c, t, g, unknown or other

<400> 58
cagatatcca gaaccctgac cctgcncgtg taccagctga gagactctaa atccagtgac       60

aagtctgtct gccta                                                        75


<210> 59
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(39)
<223> a, c, t, g, unknown or other

<400> 59
cagatatcca gaaccctgac cctgcnnnnn nnnnnnnnnc gtgtaccagc tgagagactc       60

taaatccagt cacaa                                                        75


<210> 60
<211> 64
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 60
cagatatcca gaaccctgac cctgctgaga gactctaaat ccagtgacaa gtctgtctgc       60

ctat                                                                    64


<210> 61
<211> 75
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      Wild-type target sequence


<400> 61
cagatatcca gaaccctgac cctgccgtgt accagctgag agactctaaa tccagtgaca       60

agtctgtctg cctat                                                        75


<210> 62
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 62
cagatatcca gaaccctgac cctgcaccag ctgagagact ctaaatccag tgacaagtct       60

gtctgcctat                                                              70


<210> 63
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 63
Leu Ile Asn Asp Arg Thr Ser Cys Thr Ser Cys Pro Ser Thr Ser Ala 
1               5                   10                  15      


Ala Gln Arg Met Cys Leu Met Arg Lys Ala Ala Gly His Pro Ala Ser 
            20                  25                  30          


Asp 
    


