                         SEQUENCE LISTING

<110>  Montana State University
 
<120>  GENE MODULATION WITH CRISPR SYSTEM TYPE I

<130>  7478-100821-02

<150>  US 62/754,268
<151>  2018-11-01

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  1284
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VP64-Cas7 construct

<400>  1
atggacgcgc tggacgattt cgatctcgac atgctgggtt ctgatgccct cgatgacttt       60

gacctggata tgttgggaag cgacgcattg gatgactttg atctggacat gctcggctcc      120

gatgctctgg acgatttcga tctcgatatg ttaattaacg gcagcgcggg atcggcagcg      180

ggcagcggag aattttctaa ctttatcaat attcatgttc tgatctctca cagcccttca      240

tgtctgaacc gcgacgatat gaacatgcag aaagacgcta ttttcggcgg caaaagacga      300

gtaagaattt caagtcaaag ccttaaacgt gcgatgcgta aaagtggtta ttacgcacaa      360

aatattggtg aatccagtct cagaaccatt catcttgcac aattacgtga tgttcttcgg      420

caaaaacttg gtgaacgttt tgaccaaaaa atcatcgata agacattagc gctgctctcc      480

ggtaaatcag ttgatgaagc cgaaaagatt tctgccgatg cggttactcc ctgggttgtg      540

ggagaaatag cctggttctg tgagcaggtt gcaaaagcag aggctgataa tctggatgat      600

aaaaagctgc tcaaagttct taaggaagat attgccgcca tacgtgtgaa tttacagcag      660

ggtgttgata ttgcgcttag tggaagaatg gcaaccagcg gcatgatgac tgagttggga      720

aaagttgatg gtgcaatgtc cattgcgcat gcgatcacta ctcatcaggt tgattctgat      780

attgactggt tcaccgctgt agatgattta caggaacaag gttctgcaca tctgggaact      840

caggaatttt catcgggtgt tttttatcgt tatgccaaca ttaacctcgc tcaacttcag      900

gaaaatttag gtggtgcctc cagggagcag gctctggaaa ttgcaaccca tgttgttcat      960

atgctggcaa cagaggtccc tggagcaaaa cagcgtactt atgccgcttt taaccctgcg     1020

gatatggtaa tggttaattt ctccgatatg ccactttcta tggcaaatgc ttttgaaaaa     1080

gcggttaaag cgaaagatgg ctttttgcaa ccgtctatac aggcgtttaa tcaatattgg     1140

gatcgcgttg ccaatggata tggtctgaac ggagctgctg cgcaattcag cttatctgat     1200

gtagacccaa ttactgctca agttaaacaa atgcctactt tagaacagtt aaaatcctgg     1260

gttcgtaata atggcgaggc gtga                                            1284


<210>  2
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VP64-Cas7 construct

<400>  2

Met Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala 
1               5                   10                  15      


Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp 
            20                  25                  30          


Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu 
        35                  40                  45              


Asp Met Leu Ile Asn Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu 
    50                  55                  60                  


Phe Ser Asn Phe Ile Asn Ile His Val Leu Ile Ser His Ser Pro Ser 
65                  70                  75                  80  


Cys Leu Asn Arg Asp Asp Met Asn Met Gln Lys Asp Ala Ile Phe Gly 
                85                  90                  95      


Gly Lys Arg Arg Val Arg Ile Ser Ser Gln Ser Leu Lys Arg Ala Met 
            100                 105                 110         


Arg Lys Ser Gly Tyr Tyr Ala Gln Asn Ile Gly Glu Ser Ser Leu Arg 
        115                 120                 125             


Thr Ile His Leu Ala Gln Leu Arg Asp Val Leu Arg Gln Lys Leu Gly 
    130                 135                 140                 


Glu Arg Phe Asp Gln Lys Ile Ile Asp Lys Thr Leu Ala Leu Leu Ser 
145                 150                 155                 160 


Gly Lys Ser Val Asp Glu Ala Glu Lys Ile Ser Ala Asp Ala Val Thr 
                165                 170                 175     


Pro Trp Val Val Gly Glu Ile Ala Trp Phe Cys Glu Gln Val Ala Lys 
            180                 185                 190         


Ala Glu Ala Asp Asn Leu Asp Asp Lys Lys Leu Leu Lys Val Leu Lys 
        195                 200                 205             


Glu Asp Ile Ala Ala Ile Arg Val Asn Leu Gln Gln Gly Val Asp Ile 
    210                 215                 220                 


Ala Leu Ser Gly Arg Met Ala Thr Ser Gly Met Met Thr Glu Leu Gly 
225                 230                 235                 240 


Lys Val Asp Gly Ala Met Ser Ile Ala His Ala Ile Thr Thr His Gln 
                245                 250                 255     


Val Asp Ser Asp Ile Asp Trp Phe Thr Ala Val Asp Asp Leu Gln Glu 
            260                 265                 270         


Gln Gly Ser Ala His Leu Gly Thr Gln Glu Phe Ser Ser Gly Val Phe 
        275                 280                 285             


Tyr Arg Tyr Ala Asn Ile Asn Leu Ala Gln Leu Gln Glu Asn Leu Gly 
    290                 295                 300                 


Gly Ala Ser Arg Glu Gln Ala Leu Glu Ile Ala Thr His Val Val His 
305                 310                 315                 320 


Met Leu Ala Thr Glu Val Pro Gly Ala Lys Gln Arg Thr Tyr Ala Ala 
                325                 330                 335     


Phe Asn Pro Ala Asp Met Val Met Val Asn Phe Ser Asp Met Pro Leu 
            340                 345                 350         


Ser Met Ala Asn Ala Phe Glu Lys Ala Val Lys Ala Lys Asp Gly Phe 
        355                 360                 365             


Leu Gln Pro Ser Ile Gln Ala Phe Asn Gln Tyr Trp Asp Arg Val Ala 
    370                 375                 380                 


Asn Gly Tyr Gly Leu Asn Gly Ala Ala Ala Gln Phe Ser Leu Ser Asp 
385                 390                 395                 400 


Val Asp Pro Ile Thr Ala Gln Val Lys Gln Met Pro Thr Leu Glu Gln 
                405                 410                 415     


Leu Lys Ser Trp Val Arg Asn Asn Gly Glu Ala 
            420                 425         


<210>  3
<211>  1266
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas7-VP64 construct

<400>  3
atgtctaact ttatcaatat tcatgttctg atctctcaca gcccttcatg tctgaaccgc       60

gacgatatga acatgcagaa agacgctatt ttcggcggca aaagacgagt aagaatttca      120

agtcaaagcc ttaaacgtgc gatgcgtaaa agtggttatt acgcacaaaa tattggtgaa      180

tccagtctca gaaccattca tcttgcacaa ttacgtgatg ttcttcggca aaaacttggt      240

gaacgttttg accaaaaaat catcgataag acattagcgc tgctctccgg taaatcagtt      300

gatgaagccg aaaagatttc tgccgatgcg gttactccct gggttgtggg agaaatagcc      360

tggttctgtg agcaggttgc aaaagcagag gctgataatc tggatgataa aaagctgctc      420

aaagttctta aggaagatat tgccgccata cgtgtgaatt tacagcaggg tgttgatatt      480

gcgcttagtg gaagaatggc aaccagcggc atgatgactg agttgggaaa agttgatggt      540

gcaatgtcca ttgcgcatgc gatcactact catcaggttg attctgatat tgactggttc      600

accgctgtag atgatttaca ggaacaaggt tctgcacatc tgggaactca ggaattttca      660

tcgggtgttt tttatcgtta tgccaacatt aacctcgctc aacttcagga aaatttaggt      720

ggtgcctcca gggagcaggc tctggaaatt gcaacccatg ttgttcatat gctggcaaca      780

gaggtccctg gagcaaaaca gcgtacttat gccgctttta accctgcgga tatggtaatg      840

gttaatttct ccgatatgcc actttctatg gcaaatgctt ttgaaaaagc ggttaaagcg      900

aaagatggct ttttgcaacc gtctatacag gcgtttaatc aatattggga tcgcgttgcc      960

aatggatatg gtctgaacgg agctgctgcg caattcagct tatctgatgt agacccaatt     1020

actgctcaag ttaaacaaat gcctacttta gaacagttaa aatcctgggt tcgtaataat     1080

ggcgaggcgc caattgccgg atccaaggct agcgacgcgc tggacgattt cgatctcgac     1140

atgctgggtt ctgatgccct cgatgacttt gacctggata tgttgggaag cgacgcattg     1200

gatgactttg atctggacat gctcggctcc gatgctctgg acgatttcga tctcgatatg     1260

ttatga                                                                1266


<210>  4
<211>  421
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas7-VP64 construct

<400>  4

Met Ser Asn Phe Ile Asn Ile His Val Leu Ile Ser His Ser Pro Ser 
1               5                   10                  15      


Cys Leu Asn Arg Asp Asp Met Asn Met Gln Lys Asp Ala Ile Phe Gly 
            20                  25                  30          


Gly Lys Arg Arg Val Arg Ile Ser Ser Gln Ser Leu Lys Arg Ala Met 
        35                  40                  45              


Arg Lys Ser Gly Tyr Tyr Ala Gln Asn Ile Gly Glu Ser Ser Leu Arg 
    50                  55                  60                  


Thr Ile His Leu Ala Gln Leu Arg Asp Val Leu Arg Gln Lys Leu Gly 
65                  70                  75                  80  


Glu Arg Phe Asp Gln Lys Ile Ile Asp Lys Thr Leu Ala Leu Leu Ser 
                85                  90                  95      


Gly Lys Ser Val Asp Glu Ala Glu Lys Ile Ser Ala Asp Ala Val Thr 
            100                 105                 110         


Pro Trp Val Val Gly Glu Ile Ala Trp Phe Cys Glu Gln Val Ala Lys 
        115                 120                 125             


Ala Glu Ala Asp Asn Leu Asp Asp Lys Lys Leu Leu Lys Val Leu Lys 
    130                 135                 140                 


Glu Asp Ile Ala Ala Ile Arg Val Asn Leu Gln Gln Gly Val Asp Ile 
145                 150                 155                 160 


Ala Leu Ser Gly Arg Met Ala Thr Ser Gly Met Met Thr Glu Leu Gly 
                165                 170                 175     


Lys Val Asp Gly Ala Met Ser Ile Ala His Ala Ile Thr Thr His Gln 
            180                 185                 190         


Val Asp Ser Asp Ile Asp Trp Phe Thr Ala Val Asp Asp Leu Gln Glu 
        195                 200                 205             


Gln Gly Ser Ala His Leu Gly Thr Gln Glu Phe Ser Ser Gly Val Phe 
    210                 215                 220                 


Tyr Arg Tyr Ala Asn Ile Asn Leu Ala Gln Leu Gln Glu Asn Leu Gly 
225                 230                 235                 240 


Gly Ala Ser Arg Glu Gln Ala Leu Glu Ile Ala Thr His Val Val His 
                245                 250                 255     


Met Leu Ala Thr Glu Val Pro Gly Ala Lys Gln Arg Thr Tyr Ala Ala 
            260                 265                 270         


Phe Asn Pro Ala Asp Met Val Met Val Asn Phe Ser Asp Met Pro Leu 
        275                 280                 285             


Ser Met Ala Asn Ala Phe Glu Lys Ala Val Lys Ala Lys Asp Gly Phe 
    290                 295                 300                 


Leu Gln Pro Ser Ile Gln Ala Phe Asn Gln Tyr Trp Asp Arg Val Ala 
305                 310                 315                 320 


Asn Gly Tyr Gly Leu Asn Gly Ala Ala Ala Gln Phe Ser Leu Ser Asp 
                325                 330                 335     


Val Asp Pro Ile Thr Ala Gln Val Lys Gln Met Pro Thr Leu Glu Gln 
            340                 345                 350         


Leu Lys Ser Trp Val Arg Asn Asn Gly Glu Ala Pro Ile Ala Gly Ser 
        355                 360                 365             


Lys Ala Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser 
    370                 375                 380                 


Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu 
385                 390                 395                 400 


Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe 
                405                 410                 415     


Asp Leu Asp Met Leu 
            420     


<210>  5
<211>  1323
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KRAB-Cas7 construct

<400>  5
atgcgcacac tggttacgtt caaggacgtg tttgtggact ttacacgtga ggagtggaaa       60

ttgctggata ctgcgcaaca aattgtgtat cgcaatgtca tgcttgagaa ttacaagaac      120

ctcgtcagtc tcggatacca gttgacgaaa ccggatgtga tccttcgcct cgaaaagggg      180

gaagaacctt ggctggtagg cagcgcggga tcggcagcgg gcagcggaga attttctaac      240

tttatcaata ttcatgttct gatctctcac agcccttcat gtctgaaccg cgacgatatg      300

aacatgcaga aagacgctat tttcggcggc aaaagacgag taagaatttc aagtcaaagc      360

cttaaacgtg cgatgcgtaa aagtggttat tacgcacaaa atattggtga atccagtctc      420

agaaccattc atcttgcaca attacgtgat gttcttcggc aaaaacttgg tgaacgtttt      480

gaccaaaaaa tcatcgataa gacattagcg ctgctctccg gtaaatcagt tgatgaagcc      540

gaaaagattt ctgccgatgc ggttactccc tgggttgtgg gagaaatagc ctggttctgt      600

gagcaggttg caaaagcaga ggctgataat ctggatgata aaaagctgct caaagttctt      660

aaggaagata ttgccgccat acgtgtgaat ttacagcagg gtgttgatat tgcgcttagt      720

ggaagaatgg caaccagcgg catgatgact gagttgggaa aagttgatgg tgcaatgtcc      780

attgcgcatg cgatcactac tcatcaggtt gattctgata ttgactggtt caccgctgta      840

gatgatttac aggaacaagg ttctgcacat ctgggaactc aggaattttc atcgggtgtt      900

ttttatcgtt atgccaacat taacctcgct caacttcagg aaaatttagg tggtgcctcc      960

agggagcagg ctctggaaat tgcaacccat gttgttcata tgctggcaac agaggtccct     1020

ggagcaaaac agcgtactta tgccgctttt aaccctgcgg atatggtaat ggttaatttc     1080

tccgatatgc cactttctat ggcaaatgct tttgaaaaag cggttaaagc gaaagatggc     1140

tttttgcaac cgtctataca ggcgtttaat caatattggg atcgcgttgc caatggatat     1200

ggtctgaacg gagctgctgc gcaattcagc ttatctgatg tagacccaat tactgctcaa     1260

gttaaacaaa tgcctacttt agaacagtta aaatcctggg ttcgtaataa tggcgaggcg     1320

tga                                                                   1323


<210>  6
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KRAB-Cas7 construct

<400>  6

Met Arg Thr Leu Val Thr Phe Lys Asp Val Phe Val Asp Phe Thr Arg 
1               5                   10                  15      


Glu Glu Trp Lys Leu Leu Asp Thr Ala Gln Gln Ile Val Tyr Arg Asn 
            20                  25                  30          


Val Met Leu Glu Asn Tyr Lys Asn Leu Val Ser Leu Gly Tyr Gln Leu 
        35                  40                  45              


Thr Lys Pro Asp Val Ile Leu Arg Leu Glu Lys Gly Glu Glu Pro Trp 
    50                  55                  60                  


Leu Val Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe Ser Asn 
65                  70                  75                  80  


Phe Ile Asn Ile His Val Leu Ile Ser His Ser Pro Ser Cys Leu Asn 
                85                  90                  95      


Arg Asp Asp Met Asn Met Gln Lys Asp Ala Ile Phe Gly Gly Lys Arg 
            100                 105                 110         


Arg Val Arg Ile Ser Ser Gln Ser Leu Lys Arg Ala Met Arg Lys Ser 
        115                 120                 125             


Gly Tyr Tyr Ala Gln Asn Ile Gly Glu Ser Ser Leu Arg Thr Ile His 
    130                 135                 140                 


Leu Ala Gln Leu Arg Asp Val Leu Arg Gln Lys Leu Gly Glu Arg Phe 
145                 150                 155                 160 


Asp Gln Lys Ile Ile Asp Lys Thr Leu Ala Leu Leu Ser Gly Lys Ser 
                165                 170                 175     


Val Asp Glu Ala Glu Lys Ile Ser Ala Asp Ala Val Thr Pro Trp Val 
            180                 185                 190         


Val Gly Glu Ile Ala Trp Phe Cys Glu Gln Val Ala Lys Ala Glu Ala 
        195                 200                 205             


Asp Asn Leu Asp Asp Lys Lys Leu Leu Lys Val Leu Lys Glu Asp Ile 
    210                 215                 220                 


Ala Ala Ile Arg Val Asn Leu Gln Gln Gly Val Asp Ile Ala Leu Ser 
225                 230                 235                 240 


Gly Arg Met Ala Thr Ser Gly Met Met Thr Glu Leu Gly Lys Val Asp 
                245                 250                 255     


Gly Ala Met Ser Ile Ala His Ala Ile Thr Thr His Gln Val Asp Ser 
            260                 265                 270         


Asp Ile Asp Trp Phe Thr Ala Val Asp Asp Leu Gln Glu Gln Gly Ser 
        275                 280                 285             


Ala His Leu Gly Thr Gln Glu Phe Ser Ser Gly Val Phe Tyr Arg Tyr 
    290                 295                 300                 


Ala Asn Ile Asn Leu Ala Gln Leu Gln Glu Asn Leu Gly Gly Ala Ser 
305                 310                 315                 320 


Arg Glu Gln Ala Leu Glu Ile Ala Thr His Val Val His Met Leu Ala 
                325                 330                 335     


Thr Glu Val Pro Gly Ala Lys Gln Arg Thr Tyr Ala Ala Phe Asn Pro 
            340                 345                 350         


Ala Asp Met Val Met Val Asn Phe Ser Asp Met Pro Leu Ser Met Ala 
        355                 360                 365             


Asn Ala Phe Glu Lys Ala Val Lys Ala Lys Asp Gly Phe Leu Gln Pro 
    370                 375                 380                 


Ser Ile Gln Ala Phe Asn Gln Tyr Trp Asp Arg Val Ala Asn Gly Tyr 
385                 390                 395                 400 


Gly Leu Asn Gly Ala Ala Ala Gln Phe Ser Leu Ser Asp Val Asp Pro 
                405                 410                 415     


Ile Thr Ala Gln Val Lys Gln Met Pro Thr Leu Glu Gln Leu Lys Ser 
            420                 425                 430         


Trp Val Arg Asn Asn Gly Glu Ala 
        435                 440 


<210>  7
<211>  1311
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas7-KRAB construct

<400>  7
atgtctaact ttatcaatat tcatgttctg atctctcaca gcccttcatg tctgaaccgc       60

gacgatatga acatgcagaa agacgctatt ttcggcggca aaagacgagt aagaatttca      120

agtcaaagcc ttaaacgtgc gatgcgtaaa agtggttatt acgcacaaaa tattggtgaa      180

tccagtctca gaaccattca tcttgcacaa ttacgtgatg ttcttcggca aaaacttggt      240

gaacgttttg accaaaaaat catcgataag acattagcgc tgctctccgg taaatcagtt      300

gatgaagccg aaaagatttc tgccgatgcg gttactccct gggttgtggg agaaatagcc      360

tggttctgtg agcaggttgc aaaagcagag gctgataatc tggatgataa aaagctgctc      420

aaagttctta aggaagatat tgccgccata cgtgtgaatt tacagcaggg tgttgatatt      480

gcgcttagtg gaagaatggc aaccagcggc atgatgactg agttgggaaa agttgatggt      540

gcaatgtcca ttgcgcatgc gatcactact catcaggttg attctgatat tgactggttc      600

accgctgtag atgatttaca ggaacaaggt tctgcacatc tgggaactca ggaattttca      660

tcgggtgttt tttatcgtta tgccaacatt aacctcgctc aacttcagga aaatttaggt      720

ggtgcctcca gggagcaggc tctggaaatt gcaacccatg ttgttcatat gctggcaaca      780

gaggtccctg gagcaaaaca gcgtacttat gccgctttta accctgcgga tatggtaatg      840

gttaatttct ccgatatgcc actttctatg gcaaatgctt ttgaaaaagc ggttaaagcg      900

aaagatggct ttttgcaacc gtctatacag gcgtttaatc aatattggga tcgcgttgcc      960

aatggatatg gtctgaacgg agctgctgcg caattcagct tatctgatgt agacccaatt     1020

actgctcaag ttaaacaaat gcctacttta gaacagttaa aatcctgggt tcgtaataat     1080

ggcgaggcgc caattgccgg atccaaggct agccgcacac tggttacgtt caaggacgtg     1140

tttgtggact ttacacgtga ggagtggaaa ttgctggata ctgcgcaaca aattgtgtat     1200

cgaaatgtca tgcttgagaa ttacaagaac ctcgtcagtc tcggatacca gttgacgaaa     1260

ccggatgtga tccttaggct cgaaaagggg gaagaacctt ggctggtatg a              1311


<210>  8
<211>  436
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas7-KRAB construct

<400>  8

Met Ser Asn Phe Ile Asn Ile His Val Leu Ile Ser His Ser Pro Ser 
1               5                   10                  15      


Cys Leu Asn Arg Asp Asp Met Asn Met Gln Lys Asp Ala Ile Phe Gly 
            20                  25                  30          


Gly Lys Arg Arg Val Arg Ile Ser Ser Gln Ser Leu Lys Arg Ala Met 
        35                  40                  45              


Arg Lys Ser Gly Tyr Tyr Ala Gln Asn Ile Gly Glu Ser Ser Leu Arg 
    50                  55                  60                  


Thr Ile His Leu Ala Gln Leu Arg Asp Val Leu Arg Gln Lys Leu Gly 
65                  70                  75                  80  


Glu Arg Phe Asp Gln Lys Ile Ile Asp Lys Thr Leu Ala Leu Leu Ser 
                85                  90                  95      


Gly Lys Ser Val Asp Glu Ala Glu Lys Ile Ser Ala Asp Ala Val Thr 
            100                 105                 110         


Pro Trp Val Val Gly Glu Ile Ala Trp Phe Cys Glu Gln Val Ala Lys 
        115                 120                 125             


Ala Glu Ala Asp Asn Leu Asp Asp Lys Lys Leu Leu Lys Val Leu Lys 
    130                 135                 140                 


Glu Asp Ile Ala Ala Ile Arg Val Asn Leu Gln Gln Gly Val Asp Ile 
145                 150                 155                 160 


Ala Leu Ser Gly Arg Met Ala Thr Ser Gly Met Met Thr Glu Leu Gly 
                165                 170                 175     


Lys Val Asp Gly Ala Met Ser Ile Ala His Ala Ile Thr Thr His Gln 
            180                 185                 190         


Val Asp Ser Asp Ile Asp Trp Phe Thr Ala Val Asp Asp Leu Gln Glu 
        195                 200                 205             


Gln Gly Ser Ala His Leu Gly Thr Gln Glu Phe Ser Ser Gly Val Phe 
    210                 215                 220                 


Tyr Arg Tyr Ala Asn Ile Asn Leu Ala Gln Leu Gln Glu Asn Leu Gly 
225                 230                 235                 240 


Gly Ala Ser Arg Glu Gln Ala Leu Glu Ile Ala Thr His Val Val His 
                245                 250                 255     


Met Leu Ala Thr Glu Val Pro Gly Ala Lys Gln Arg Thr Tyr Ala Ala 
            260                 265                 270         


Phe Asn Pro Ala Asp Met Val Met Val Asn Phe Ser Asp Met Pro Leu 
        275                 280                 285             


Ser Met Ala Asn Ala Phe Glu Lys Ala Val Lys Ala Lys Asp Gly Phe 
    290                 295                 300                 


Leu Gln Pro Ser Ile Gln Ala Phe Asn Gln Tyr Trp Asp Arg Val Ala 
305                 310                 315                 320 


Asn Gly Tyr Gly Leu Asn Gly Ala Ala Ala Gln Phe Ser Leu Ser Asp 
                325                 330                 335     


Val Asp Pro Ile Thr Ala Gln Val Lys Gln Met Pro Thr Leu Glu Gln 
            340                 345                 350         


Leu Lys Ser Trp Val Arg Asn Asn Gly Glu Ala Pro Ile Ala Gly Ser 
        355                 360                 365             


Lys Ala Ser Arg Thr Leu Val Thr Phe Lys Asp Val Phe Val Asp Phe 
    370                 375                 380                 


Thr Arg Glu Glu Trp Lys Leu Leu Asp Thr Ala Gln Gln Ile Val Tyr 
385                 390                 395                 400 


Arg Asn Val Met Leu Glu Asn Tyr Lys Asn Leu Val Ser Leu Gly Tyr 
                405                 410                 415     


Gln Leu Thr Lys Pro Asp Val Ile Leu Arg Leu Glu Lys Gly Glu Glu 
            420                 425                 430         


Pro Trp Leu Val 
        435     


<210>  9
<211>  1806
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  emGFP-Cas7 construct

<400>  9
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtct      720

aactttatca atattcatgt tctgatctct cacagccctt catgtctgaa ccgcgacgat      780

atgaacatgc agaaagacgc tattttcggc ggcaaaagac gagtaagaat ttcaagtcaa      840

agccttaaac gtgcgatgcg taaaagtggt tattacgcac aaaatattgg tgaatccagt      900

ctcagaacca ttcatcttgc acaattacgt gatgttcttc ggcaaaaact tggtgaacgt      960

tttgaccaaa aaatcatcga taagacatta gcgctgctct ccggtaaatc agttgatgaa     1020

gccgaaaaga tttctgccga tgcggttact ccctgggttg tgggagaaat agcctggttc     1080

tgtgagcagg ttgcaaaagc agaggctgat aatctggatg ataaaaagct gctcaaagtt     1140

cttaaggaag atattgccgc catacgtgtg aatttacagc agggtgttga tattgcgctt     1200

agtggaagaa tggcaaccag cggcatgatg actgagttgg gaaaagttga tggtgcaatg     1260

tccattgcgc atgcgatcac tactcatcag gttgattctg atattgactg gttcaccgct     1320

gtagatgatt tacaggaaca aggttctgca catctgggaa ctcaggaatt ttcatcgggt     1380

gttttttatc gttatgccaa cattaacctc gctcaacttc aggaaaattt aggtggtgcc     1440

tccagggagc aggctctgga aattgcaacc catgttgttc atatgctggc aacagaggtc     1500

cctggagcaa aacagcgtac ttatgccgct tttaaccctg cggatatggt aatggttaat     1560

ttctccgata tgccactttc tatggcaaat gcttttgaaa aagcggttaa agcgaaagat     1620

ggctttttgc aaccgtctat acaggcgttt aatcaatatt gggatcgcgt tgccaatgga     1680

tatggtctga acggagctgc tgcgcaattc agcttatctg atgtagaccc aattactgct     1740

caagttaaac aaatgcctac tttagaacag ttaaaatcct gggttcgtaa taatggcgag     1800

gcgtga                                                                1806


<210>  10
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  emGFP-Cas7 construct

<400>  10

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Lys Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Thr Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Ser 
225                 230                 235                 240 


Asn Phe Ile Asn Ile His Val Leu Ile Ser His Ser Pro Ser Cys Leu 
                245                 250                 255     


Asn Arg Asp Asp Met Asn Met Gln Lys Asp Ala Ile Phe Gly Gly Lys 
            260                 265                 270         


Arg Arg Val Arg Ile Ser Ser Gln Ser Leu Lys Arg Ala Met Arg Lys 
        275                 280                 285             


Ser Gly Tyr Tyr Ala Gln Asn Ile Gly Glu Ser Ser Leu Arg Thr Ile 
    290                 295                 300                 


His Leu Ala Gln Leu Arg Asp Val Leu Arg Gln Lys Leu Gly Glu Arg 
305                 310                 315                 320 


Phe Asp Gln Lys Ile Ile Asp Lys Thr Leu Ala Leu Leu Ser Gly Lys 
                325                 330                 335     


Ser Val Asp Glu Ala Glu Lys Ile Ser Ala Asp Ala Val Thr Pro Trp 
            340                 345                 350         


Val Val Gly Glu Ile Ala Trp Phe Cys Glu Gln Val Ala Lys Ala Glu 
        355                 360                 365             


Ala Asp Asn Leu Asp Asp Lys Lys Leu Leu Lys Val Leu Lys Glu Asp 
    370                 375                 380                 


Ile Ala Ala Ile Arg Val Asn Leu Gln Gln Gly Val Asp Ile Ala Leu 
385                 390                 395                 400 


Ser Gly Arg Met Ala Thr Ser Gly Met Met Thr Glu Leu Gly Lys Val 
                405                 410                 415     


Asp Gly Ala Met Ser Ile Ala His Ala Ile Thr Thr His Gln Val Asp 
            420                 425                 430         


Ser Asp Ile Asp Trp Phe Thr Ala Val Asp Asp Leu Gln Glu Gln Gly 
        435                 440                 445             


Ser Ala His Leu Gly Thr Gln Glu Phe Ser Ser Gly Val Phe Tyr Arg 
    450                 455                 460                 


Tyr Ala Asn Ile Asn Leu Ala Gln Leu Gln Glu Asn Leu Gly Gly Ala 
465                 470                 475                 480 


Ser Arg Glu Gln Ala Leu Glu Ile Ala Thr His Val Val His Met Leu 
                485                 490                 495     


Ala Thr Glu Val Pro Gly Ala Lys Gln Arg Thr Tyr Ala Ala Phe Asn 
            500                 505                 510         


Pro Ala Asp Met Val Met Val Asn Phe Ser Asp Met Pro Leu Ser Met 
        515                 520                 525             


Ala Asn Ala Phe Glu Lys Ala Val Lys Ala Lys Asp Gly Phe Leu Gln 
    530                 535                 540                 


Pro Ser Ile Gln Ala Phe Asn Gln Tyr Trp Asp Arg Val Ala Asn Gly 
545                 550                 555                 560 


Tyr Gly Leu Asn Gly Ala Ala Ala Gln Phe Ser Leu Ser Asp Val Asp 
                565                 570                 575     


Pro Ile Thr Ala Gln Val Lys Gln Met Pro Thr Leu Glu Gln Leu Lys 
            580                 585                 590         


Ser Trp Val Arg Asn Asn Gly Glu Ala 
        595                 600     


<210>  11
<211>  1821
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas7-emGFP construct

<400>  11
atgtctaact ttatcaatat tcatgttctg atctctcaca gcccttcatg tctgaaccgc       60

gacgatatga acatgcagaa agacgctatt ttcggcggca aaagacgagt aagaatttca      120

agtcaaagcc ttaaacgtgc gatgcgtaaa agtggttatt acgcacaaaa tattggtgaa      180

tccagtctca gaaccattca tcttgcacaa ttacgtgatg ttcttcggca aaaacttggt      240

gaacgttttg accaaaaaat catcgataag acattagcgc tgctctccgg taaatcagtt      300

gatgaagccg aaaagatttc tgccgatgcg gttactccct gggttgtggg agaaatagcc      360

tggttctgtg agcaggttgc aaaagcagag gctgataatc tggatgataa aaagctgctc      420

aaagttctta aggaagatat tgccgccata cgtgtgaatt tacagcaggg tgttgatatt      480

gcgcttagtg gaagaatggc aaccagcggc atgatgactg agttgggaaa agttgatggt      540

gcaatgtcca ttgcgcatgc gatcactact catcaggttg attctgatat tgactggttc      600

accgctgtag atgatttaca ggaacaaggt tctgcacatc tgggaactca ggaattttca      660

tcgggtgttt tttatcgtta tgccaacatt aacctcgctc aacttcagga aaatttaggt      720

ggtgcctcca gggagcaggc tctggaaatt gcaacccatg ttgttcatat gctggcaaca      780

gaggtccctg gagcaaaaca gcgtacttat gccgctttta accctgcgga tatggtaatg      840

gttaatttct ccgatatgcc actttctatg gcaaatgctt ttgaaaaagc ggttaaagcg      900

aaagatggct ttttgcaacc gtctatacag gcgtttaatc aatattggga tcgcgttgcc      960

aatggatatg gtctgaacgg agctgctgcg caattcagct tatctgatgt agacccaatt     1020

actgctcaag ttaaacaaat gcctacttta gaacagttaa aatcctgggt tcgtaataat     1080

ggcgaggcgg gcggtagcgg tggcgtgagc aagggcgagg agctgttcac cggggtggtg     1140

cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag     1200

ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag     1260

ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc     1320

cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac     1380

gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg     1440

aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag     1500

gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc     1560

accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag     1620

gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc     1680

gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac     1740

gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc     1800

atggacgagc tgtacaagtg a                                               1821


<210>  12
<211>  606
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas7-emGFP construct

<400>  12

Met Ser Asn Phe Ile Asn Ile His Val Leu Ile Ser His Ser Pro Ser 
1               5                   10                  15      


Cys Leu Asn Arg Asp Asp Met Asn Met Gln Lys Asp Ala Ile Phe Gly 
            20                  25                  30          


Gly Lys Arg Arg Val Arg Ile Ser Ser Gln Ser Leu Lys Arg Ala Met 
        35                  40                  45              


Arg Lys Ser Gly Tyr Tyr Ala Gln Asn Ile Gly Glu Ser Ser Leu Arg 
    50                  55                  60                  


Thr Ile His Leu Ala Gln Leu Arg Asp Val Leu Arg Gln Lys Leu Gly 
65                  70                  75                  80  


Glu Arg Phe Asp Gln Lys Ile Ile Asp Lys Thr Leu Ala Leu Leu Ser 
                85                  90                  95      


Gly Lys Ser Val Asp Glu Ala Glu Lys Ile Ser Ala Asp Ala Val Thr 
            100                 105                 110         


Pro Trp Val Val Gly Glu Ile Ala Trp Phe Cys Glu Gln Val Ala Lys 
        115                 120                 125             


Ala Glu Ala Asp Asn Leu Asp Asp Lys Lys Leu Leu Lys Val Leu Lys 
    130                 135                 140                 


Glu Asp Ile Ala Ala Ile Arg Val Asn Leu Gln Gln Gly Val Asp Ile 
145                 150                 155                 160 


Ala Leu Ser Gly Arg Met Ala Thr Ser Gly Met Met Thr Glu Leu Gly 
                165                 170                 175     


Lys Val Asp Gly Ala Met Ser Ile Ala His Ala Ile Thr Thr His Gln 
            180                 185                 190         


Val Asp Ser Asp Ile Asp Trp Phe Thr Ala Val Asp Asp Leu Gln Glu 
        195                 200                 205             


Gln Gly Ser Ala His Leu Gly Thr Gln Glu Phe Ser Ser Gly Val Phe 
    210                 215                 220                 


Tyr Arg Tyr Ala Asn Ile Asn Leu Ala Gln Leu Gln Glu Asn Leu Gly 
225                 230                 235                 240 


Gly Ala Ser Arg Glu Gln Ala Leu Glu Ile Ala Thr His Val Val His 
                245                 250                 255     


Met Leu Ala Thr Glu Val Pro Gly Ala Lys Gln Arg Thr Tyr Ala Ala 
            260                 265                 270         


Phe Asn Pro Ala Asp Met Val Met Val Asn Phe Ser Asp Met Pro Leu 
        275                 280                 285             


Ser Met Ala Asn Ala Phe Glu Lys Ala Val Lys Ala Lys Asp Gly Phe 
    290                 295                 300                 


Leu Gln Pro Ser Ile Gln Ala Phe Asn Gln Tyr Trp Asp Arg Val Ala 
305                 310                 315                 320 


Asn Gly Tyr Gly Leu Asn Gly Ala Ala Ala Gln Phe Ser Leu Ser Asp 
                325                 330                 335     


Val Asp Pro Ile Thr Ala Gln Val Lys Gln Met Pro Thr Leu Glu Gln 
            340                 345                 350         


Leu Lys Ser Trp Val Arg Asn Asn Gly Glu Ala Gly Gly Ser Gly Gly 
        355                 360                 365             


Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
    370                 375                 380                 


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
385                 390                 395                 400 


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
                405                 410                 415     


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
            420                 425                 430         


Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
        435                 440                 445             


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
    450                 455                 460                 


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
465                 470                 475                 480 


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
                485                 490                 495     


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
            500                 505                 510         


Tyr Asn Ser His Lys Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly 
        515                 520                 525             


Ile Lys Val Asn Phe Lys Thr Arg His Asn Ile Glu Asp Gly Ser Val 
    530                 535                 540                 


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
545                 550                 555                 560 


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
                565                 570                 575     


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
            580                 585                 590         


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        595                 600                 605     


<210>  13
<211>  6864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCDF NLS-Cse1, Strep II-tag-C3 cleavage-Cse2, Cas7-linker-VP64

<400>  13
ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag       60

gagatatacc atgccgaaaa aaaaacgcaa agtgaatttg cttattgata actggatccc      120

tgtacgcccg cgaaacgggg ggaaagtcca aatcataaat ctgcaatcgc tatactgcag      180

tagagatcag tggcgattaa gtttgccccg tgacgatatg gaactggccg ctttagcact      240

gctggtttgc attgggcaaa ttatcgcccc ggcaaaagat gacgttgaat ttcgacatcg      300

cataatgaat ccgctcactg aagatgagtt tcaacaactc atcgcgccgt ggatagatat      360

gttctacctt aatcacgcag aacatccctt tatgcagacc aaaggtgtca aagcaaatga      420

tgtgactcca atggaaaaac tgttggctgg ggtaagcggc gcgacgaatt gtgcatttgt      480

caatcaaccg gggcagggtg aagcattatg tggtggatgc actgcgattg cgttattcaa      540

ccaggcgaat caggcaccag gttttggtgg tggttttaaa agcggtttac gtggaggaac      600

acctgtaaca acgttcgtac gtgggatcga tcttcgttca acggtgttac tcaatgtcct      660

cacattacct cgtcttcaaa aacaatttcc taatgaatca catacggaaa accaacctac      720

ctggattaaa cctatcaagt ccaatgagtc tatacctgct tcgtcaattg ggtttgtccg      780

tggtctattc tggcaaccag cgcatattga attatgcgat cccattggga ttggtaaatg      840

ttcttgctgt ggacaggaaa gcaatttgcg ttataccggt tttcttaagg aaaaatttac      900

ctttacagtt aatgggctat ggccccatcc gcattcccct tgtctggtaa cagtcaagaa      960

aggggaggtt gaggaaaaat ttcttgcttt caccacctcc gcaccatcat ggacacaaat     1020

cagccgagtt gtggtagata agattattca aaatgaaaat ggaaatcgcg tggcggcggt     1080

tgtgaatcaa ttcagaaata ttgcgccgca aagtcctctt gaattgatta tggggggata     1140

tcgtaataat caagcatcta ttcttgaacg gcgtcatgat gtgttgatgt ttaatcaggg     1200

gtggcaacaa tacggcaatg tgataaacga aatagtgact gttggtttgg gatataaaac     1260

agccttacgc aaggcgttat atacctttgc agaagggttt aaaaataaag acttcaaagg     1320

ggccggagtc tctgttcatg agactgcaga aaggcatttc tatcgacaga gtgaattatt     1380

aattcccgat gtactggcga atgttaattt ttcccaggct gatgaggtaa tagctgattt     1440

acgagacaaa cttcatcaat tgtgtgaaat gctatttaat caatctgtag ctccctatgc     1500

acatcatcct aaattaataa gcacattagc gcttgcccgc gccacgctat acaaacattt     1560

acgggagtta aaaccgcaag gagggccatc aaatggctga ctggagccac ccgcagttcg     1620

aaaagggtgc acttgaagtc ctctttcagg gacccgggta ccagatggct gatgaaattg     1680

atgcaatggc tttatatcga gcctggcaac aactggataa tggatcatgt gcgcaaatta     1740

gacgtgtttc agaacctgat gaattacgcg atatccctgc gttttatagg ctggtgcaac     1800

cttttggttg ggaaaaccca cgtcaccagc aggctctttt gcgcatggtg ttttgcctga     1860

gcgcaggaaa gaatgtcatc cgacatcagg acaaaaaatc ggagcaaaca acaggtatct     1920

cgttgggaag agctttagcc aatagtggaa gaattaacga gcgccgtatc tttcaattaa     1980

ttcgggctga cagaacagcc gatatggtcc agttacgtcg attacttact cacgccgaac     2040

ccgtacttga ctggccatta atggccagga tgttgacctg gtggggaaag cgcgaacgcc     2100

agcaacttct ggaagatttt gtattgacca caaacaaaaa tgcgtaagga aacctttcta     2160

tgtctaactt tatcaatatt catgttctga tctctcacag cccttcatgt ctgaaccgcg     2220

acgatatgaa catgcagaaa gacgctattt tcggcggcaa aagacgagta agaatttcaa     2280

gtcaaagcct taaacgtgcg atgcgtaaaa gtggttatta cgcacaaaat attggtgaat     2340

ccagtctcag aaccattcat cttgcacaat tacgtgatgt tcttcggcaa aaacttggtg     2400

aacgttttga ccaaaaaatc atcgataaga cattagcgct gctctccggt aaatcagttg     2460

atgaagccga aaagatttct gccgatgcgg ttactccctg ggttgtggga gaaatagcct     2520

ggttctgtga gcaggttgca aaagcagagg ctgataatct ggatgataaa aagctgctca     2580

aagttcttaa ggaagatatt gccgccatac gtgtgaattt acagcagggt gttgatattg     2640

cgcttagtgg aagaatggca accagcggca tgatgactga gttgggaaaa gttgatggtg     2700

caatgtccat tgcgcatgcg atcactactc atcaggttga ttctgatatt gactggttca     2760

ccgctgtaga tgatttacag gaacaaggtt ctgcacatct gggaactcag gaattttcat     2820

cgggtgtttt ttatcgttat gccaacatta acctcgctca acttcaggaa aatttaggtg     2880

gtgcctccag ggagcaggct ctggaaattg caacccatgt tgttcatatg ctggcaacag     2940

aggtccctgg agcaaaacag cgtacttatg ccgcttttaa ccctgcggat atggtaatgg     3000

ttaatttctc cgatatgcca ctttctatgg caaatgcttt tgaaaaagcg gttaaagcga     3060

aagatggctt tttgcaaccg tctatacagg cgtttaatca atattgggat cgcgttgcca     3120

atggatatgg tctgaacgga gctgctgcgc aattcagctt atctgatgta gacccaatta     3180

ctgctcaagt taaacaaatg cctactttag aacagttaaa atcctgggtt cgtaataatg     3240

gcgaggcgcc aattgccgga tccaaggcta gcgacgcgct ggacgatttc gatctcgaca     3300

tgctgggttc tgatgccctc gatgactttg acctggatat gttgggaagc gacgcattgg     3360

atgactttga tctggacatg ctcggctccg atgctctgga cgatttcgat ctcgatatgt     3420

tatgaacgcg gccgcactcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca     3480

gcacatggac tcgtctacta gcgcagctta attaacctag gctgctgcca ccgctgagca     3540

ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc     3600

tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca     3660

gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcatcgtg     3720

gccggatctt gcggcccctc ggcttgaacg aattgttaga cattatttgc cgactacctt     3780

ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa     3840

gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg     3900

ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt     3960

tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca     4020

gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc     4080

aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct     4140

tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc     4200

aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca     4260

cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc     4320

tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc     4380

aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc     4440

cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac     4500

gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatactctt     4560

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     4620

tgaatgtatt tagaaaaata aacaaatagc tagctcactc ggtcgctacg ctccgggcgt     4680

gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca     4740

ggggactaac atgtgaggca aaacagcagg gccgcgccgg tggcgttttt ccataggctc     4800

cgccctcctg ccagagttca cataaacaga cgcttttccg gtgcatctgt gggagccgtg     4860

aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc     4920

gtttccggcg ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata     4980

cctgttccgc ctttctccct tacgggaagt gtggcgcttt ctcatagctc acacactggt     5040

atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc     5100

agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg gaaaagcacg     5160

gtaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct     5220

aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt     5280

ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac     5340

cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca     5400

gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgctc tagatttcag     5460

tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat     5520

tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa     5580

gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg     5640

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca     5700

acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg     5760

agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt     5820

ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat     5880

aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca     5940

gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca     6000

tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg     6060

cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat     6120

gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga     6180

tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg     6240

agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat     6300

tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca     6360

gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc     6420

ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg     6480

ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca     6540

acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca     6600

tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc     6660

gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt     6720

tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg     6780

ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt     6840

aggaaattaa tacgactcac tata                                            6864


<210>  14
<211>  7564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET52 StrepII-Cas7, Cas5, Cas6

<400>  14
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat      480

ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt      540

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt      600

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc      660

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg      720

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc      780

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg      840

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca      900

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga      960

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     1020

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     1080

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     1140

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     1200

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     1260

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     1320

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     1380

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     1440

atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg     1500

atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac     1560

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1620

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1680

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1740

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1800

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1860

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1920

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1980

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     2040

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     2100

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2160

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2220

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     2280

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     2340

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt     2400

attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac     2460

aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg     2520

gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     2580

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     2640

ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg     2700

tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga     2760

agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg     2820

gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa     2880

cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt     2940

tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt     3000

caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct     3060

gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac     3120

gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag     3180

cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc     3240

cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc     3300

caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc     3360

taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga     3420

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     3480

attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt     3540

caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg     3600

aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc     3660

gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat     3720

tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt     3780

cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc     3840

tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc     3900

cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag     3960

atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt     4020

ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat     4080

ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag     4140

attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac     4200

gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg     4260

cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg     4320

tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt     4380

tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc     4440

ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact     4500

ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg     4560

gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga     4620

ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc     4680

ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt     4740

ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg     4800

tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc     4860

ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag     4920

aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag     4980

ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagat gtctaacttt     5040

atcaatattc atgttctgat ctctcacagc ccttcatgtc tgaaccgcga cgatatgaac     5100

atgcagaaag acgctatttt cggcggcaaa agacgagtaa gaatttcaag tcaaagcctt     5160

aaacgtgcga tgcgtaaaag tggttattac gcacaaaata ttggtgaatc cagtctcaga     5220

accattcatc ttgcacaatt acgtgatgtt cttcggcaaa aacttggtga acgttttgac     5280

caaaaaatca tcgataagac attagcgctg ctctccggta aatcagttga tgaagccgaa     5340

aagatttctg ccgatgcggt tactccctgg gttgtgggag aaatagcctg gttctgtgag     5400

caggttgcaa aagcagaggc tgataatctg gatgataaaa agctgctcaa agttcttaag     5460

gaagatattg ccgccatacg tgtgaattta cagcagggtg ttgatattgc gcttagtgga     5520

agaatggcaa ccagcggcat gatgactgag ttgggaaaag ttgatggtgc aatgtccatt     5580

gcgcatgcga tcactactca tcaggttgat tctgatattg actggttcac cgctgtagat     5640

gatttacagg aacaaggttc tgcacatctg ggaactcagg aattttcatc gggtgttttt     5700

tatcgttatg ccaacattaa cctcgctcaa cttcaggaaa atttaggtgg tgcctccagg     5760

gagcaggctc tggaaattgc aacccatgtt gttcatatgc tggcaacaga ggtccctgga     5820

gcaaaacagc gtacttatgc cgcttttaac cctgcggata tggtaatggt taatttctcc     5880

gatatgccac tttctatggc aaatgctttt gaaaaagcgg ttaaagcgaa agatggcttt     5940

ttgcaaccgt ctatacaggc gtttaatcaa tattgggatc gcgttgccaa tggatatggt     6000

ctgaacggag ctgctgcgca attcagctta tctgatgtag acccaattac tgctcaagtt     6060

aaacaaatgc ctactttaga acagttaaaa tcctgggttc gtaataatgg cgaggcgtga     6120

acatgagatc ttatttgatc ttgcggcttg ctgggccaat gcaagcctgg gggcagccga     6180

cctttgaagg aacgcgacct accggaagat ttccgacccg aagcgggtta ttagggctac     6240

tcggggcttg tcttgggatc caacgtgatg atacttcttc attacaggcg ttatcagaga     6300

gtgtgcaatt tgcagtgcgc tgcgatgaac tcattcttga cgatcgtcgt gtgtctgtaa     6360

cggggttgcg tgattaccat acagtccttg gagcgcgaga agattaccgt ggtttgaaaa     6420

gtcatgaaac gattcaaaca tggcgcgaat atttatgtga tgcctccttt accgtcgctc     6480

tctggttaac accccatgca acgatggtta tctcagaact tgaaaaagca gtattaaagc     6540

ctcggtatac accttacctg gggcggagaa gttgcccact aacacacccg ctttttttgg     6600

ggacatgtca ggcatcggat cctcagaagg cgctattaaa ttatgagccc gttggcggcg     6660

atatatatag tgaggaatca gttacagggc atcatttaaa atttacggcg cgcgacgaac     6720

cgatgatcac cttgcctcga caatttgctt cccgagaatg gtatgtgatt aaaggaggta     6780

tggatgtatc tcagtaaagt catcattgcc agggcctgga gcagggatct ttaccaactt     6840

caccagggat tatggcattt atttccaaac agaccggatg ctgctcgtga ttttcttttt     6900

catgttgaga agcgaaacac accagaaggc tgtcatgttt tattgcagtc agcgcaaatg     6960

cctgtttcaa ctgccgttgc gacagtcatt aaaactaaac aggttgaatt tcaacttcag     7020

gttggtgttc cactctattt tcggcttcgg gcaaatccga tcaaaactat tctcgacaat     7080

caaaagcgcc tggacagtaa agggaatatt aaacgctgtc gggttccgtt aataaaagaa     7140

gcagaacaaa tcgcgtggtt gcaacgtaaa ttgggcaatg cggcgcgcgt tgaagatgtg     7200

catcccatat cggaacggcc acagtatttt tctggtgatg gtaaaagtgg aaagatccaa     7260

acggtttgct ttgaaggtgt gctcaccatc aacgacgcgc cagcgttaat agatcttgta     7320

cagcaaggta ttgggccagc taaatcgatg ggatgtggct tgctatcttt ggctccactg     7380

tgagcggccg cagagctcgc tctggtgcca cgcggtagtt ccgctcatca ccaccatcat     7440

caccatcacc accactaatt aacctaggct gctgccaccg ctgagcaata actagcataa     7500

ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc     7560

ggat                                                                  7564


<210>  15
<211>  6413
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pET52 Cas5-Cas6-NLS

<400>  15
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat      480

ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt      540

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt      600

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc      660

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg      720

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc      780

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg      840

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca      900

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga      960

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     1020

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     1080

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     1140

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     1200

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     1260

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     1320

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     1380

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     1440

atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg     1500

atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac     1560

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1620

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1680

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1740

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1800

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1860

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1920

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1980

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     2040

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     2100

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2160

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2220

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     2280

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     2340

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt     2400

attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac     2460

aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg     2520

gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     2580

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     2640

ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg     2700

tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga     2760

agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg     2820

gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa     2880

cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt     2940

tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt     3000

caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct     3060

gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac     3120

gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag     3180

cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc     3240

cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc     3300

caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc     3360

taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga     3420

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt     3480

attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt     3540

caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg     3600

aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc     3660

gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat     3720

tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt     3780

cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc     3840

tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc     3900

cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag     3960

atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt     4020

ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat     4080

ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag     4140

attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac     4200

gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg     4260

cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg     4320

tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt     4380

tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc     4440

ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact     4500

ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg     4560

gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga     4620

ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc     4680

ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt     4740

ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg     4800

tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc     4860

ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag     4920

aaataatttt gtttaacttt aagaaggaga tataccatgg caagatctta tttgatcttg     4980

cggcttgctg ggccaatgca agcctggggg cagccgacct ttgaaggaac gcgacctacc     5040

ggaagatttc cgacccgaag cgggttatta gggctactcg gggcttgtct tgggatccaa     5100

cgtgatgata cttcttcatt acaggcgtta tcagagagtg tgcaatttgc agtgcgctgc     5160

gatgaactca ttcttgacga tcgtcgtgtg tctgtaacgg ggttgcgtga ttaccataca     5220

gtccttggag cgcgagaaga ttaccgtggt ttgaaaagtc atgaaacgat tcaaacatgg     5280

cgcgaatatt tatgtgatgc ctcctttacc gtcgctctct ggttaacacc ccatgcaacg     5340

atggttatct cagaacttga aaaagcagta ttaaagcctc ggtatacacc ttacctgggg     5400

cggagaagtt gcccactaac acacccgctt tttttgggga catgtcaggc atcggatcct     5460

cagaaggcgc tattaaatta tgagcccgtt ggcggcgata tatatagtga ggaatcagtt     5520

acagggcatc atttaaaatt tacggcgcgc gacgaaccga tgatcacctt gcctcgacaa     5580

tttgcttccc gagaatggta tgtgattaaa ggaggtatgg atgtatctca gtaaagtcat     5640

cattgccagg gcctggagca gggatcttta ccaacttcac cagggattat ggcatttatt     5700

tccaaacaga ccggatgctg ctcgtgattt tctttttcat gttgagaagc gaaacacacc     5760

agaaggctgt catgttttat tgcagtcagc gcaaatgcct gtttcaactg ccgttgcgac     5820

agtcattaaa actaaacagg ttgaatttca acttcaggtt ggtgttccac tctattttcg     5880

gcttcgggca aatccgatca aaactattct cgacaatcaa aagcgcctgg acagtaaagg     5940

gaatattaaa cgctgtcggg ttccgttaat aaaagaagca gaacaaatcg cgtggttgca     6000

acgtaaattg ggcaatgcgg cgcgcgttga agatgtgcat cccatatcgg aacggccaca     6060

gtatttttct ggtgatggta aaagtggaaa gatccaaacg gtttgctttg aaggtgtgct     6120

caccatcaac gacgcgccag cgttaataga tcttgtacag caaggtattg ggccagctaa     6180

atcgatggga tgtggcttgc tatctttggc tccactgccg aaaaaaaaac gcaaagtgtg     6240

agagctcgct ctggtgccac gcggtagttc cgctcatcac caccatcatc accatcacca     6300

ccactaatta acctaggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc     6360

ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat            6413


