                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
       Meshinchi, Soheil
       Kaeding, Allison June
       Le, Quy H.
 
<120>  ANTIBODY-DRUG CONJUGATE FOR TREATING ACUTE MYELOID LEUKEMIA

<130>  FHTM-1-70537

<150>  US 62/753,747
<151>  2018-10-31

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  2187
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(2187)
<223>  DNA/RNA Hybrid

<400>  1
tgccaggctc tccaccccca cttcccaatt gaggaaaccg aggcagagga ggctcagcgc       60

cacgcactcc tctttctgcc tggccggcca ctcccgtctg ctgtgacgcg cggacagaga      120

gctaccggtg gacccacggt gcctccctcc ctgggatcta cacagaccat ggccttgcca      180

acggctcgac ccctgttggg gtcctgtggg acccccgccc tcggcagcct cctgttcctg      240

ctcttcagcc tcggatgggt gcagccctcg aggaccctgg ctggagagac agggcaggag      300

gctgcgcccc tggacggagt cctggccaac ccacctaaca tttccagcct ctcccctcgc      360

caactccttg gcttcccgtg tgcggaggtg tccggcctga gcacggagcg tgtccgggag      420

ctggctgtgg ccttggcaca gaagaatgtc aagctctcaa cagagcagct gcgctgtctg      480

gctcaccggc tctctgagcc ccccgaggac ctggacgccc tcccattgga cctgctgcta      540

ttcctcaacc cagatgcgtt ctcggggccc caggcctgca cccatttctt ctcccgcatc      600

acgaaagcca atgtggacct gctcccgagg ggggctcccg agcgacagcg gctgctgcct      660

gcggctctgg cctgctgggg tgtgcggggg tctctgctga gcgaggctga tgtgcgggct      720

ctgggaggcc tggcttgcga cctgcctggg cgctttgtgg ccgagtcggc cgaagtgctg      780

ctaccccggc tggtgagctg cccgggaccc ctggaccagg accagcagga ggcagccagg      840

gcggctctgc agggcggggg acccccctac ggccccccgt cgacatggtc tgtctccacg      900

atggacgctc tgcggggcct gctgcccgtg ctgggccagc ccatcatccg cagcatcccg      960

cagggcatcg tggccgcgtg gcggcaacgc tcctctcggg acccatcctg gcggcagcct     1020

gaacggacca tcctccggcc gcggttccgg cgggaagtgg agaagacagc ctgtccttca     1080

ggcaagaagg cccgcgagat agacgagagc ctcatcttct acaagaagtg ggagctggaa     1140

gcctgcgtgg atgcggccct gctggccacc cagatggacc gcgtgaacgc catccccttc     1200

acctacgagc agctggacgt cctaaagcat aaactggatg agctctaccc acaaggttac     1260

cccgagtctg tgatccagca cctgggctac ctcttcctca agatgagccc tgaggacatt     1320

cgcaagtgga atgtgacgtc cctggagacc ctgaaggctt tgcttgaagt caacaaaggg     1380

cacgaaatga gtcctcaggt ggccaccctg atcgaccgct ttgtgaaggg aaggggccag     1440

ctagacaaag acaccctaga caccctgacc gccttctacc ctgggtacct gtgctccctc     1500

agccccgagg agctgagctc cgtgcccccc agcagcatct gggcggtcag gccccaggac     1560

ctggacacgt gtgacccaag gcagctggac gtcctctatc ccaaggcccg ccttgctttc     1620

cagaacatga acgggtccga atacttcgtg aagatccagt ccttcctggg tggggccccc     1680

acggaggatt tgaaggcgct tagtcagcag aatgtgagca tggacttggc cacgttcatg     1740

aagctgcgga cggatgcggt gctgccgttg actgtggctg aggtgcagaa acttctggga     1800

ccccacgtgg agggcctgaa ggcggaggag cggcaccgcc cggtgcggga ctggatccta     1860

cggcagcggc aggacgacct ggacacgctg gggctggggc tacagggcgg catccccaac     1920

ggctacctgg tcctagacct cagcgtgcaa gaggccctct cggggacgcc ctgcctccta     1980

ggacctggac ctgttctcac cgtcctggca ctgctcctag cctccaccct ggcctgaggg     2040

ccccactccc ttgctggccc cagccctgct ggggatcccc gcctggccag gagcaggcac     2100

gggtggtccc tgttccaccc caagagaact cgcgctcagt aaacgggaac atgccccctg     2160

caaaaaaaaa aaaaaaaaaa aaaaaaa                                         2187


<210>  2
<211>  622
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Leu Pro Thr Ala Arg Pro Leu Leu Gly Ser Cys Gly Thr Pro 
1               5                   10                  15      


Ala Leu Gly Ser Leu Leu Phe Leu Leu Phe Ser Leu Gly Trp Val Gln 
            20                  25                  30          


Pro Ser Arg Thr Leu Ala Gly Glu Thr Gly Gln Glu Ala Ala Pro Leu 
        35                  40                  45              


Asp Gly Val Leu Ala Asn Pro Pro Asn Ile Ser Ser Leu Ser Pro Arg 
    50                  55                  60                  


Gln Leu Leu Gly Phe Pro Cys Ala Glu Val Ser Gly Leu Ser Thr Glu 
65                  70                  75                  80  


Arg Val Arg Glu Leu Ala Val Ala Leu Ala Gln Lys Asn Val Lys Leu 
                85                  90                  95      


Ser Thr Glu Gln Leu Arg Cys Leu Ala His Arg Leu Ser Glu Pro Pro 
            100                 105                 110         


Glu Asp Leu Asp Ala Leu Pro Leu Asp Leu Leu Leu Phe Leu Asn Pro 
        115                 120                 125             


Asp Ala Phe Ser Gly Pro Gln Ala Cys Thr His Phe Phe Ser Arg Ile 
    130                 135                 140                 


Thr Lys Ala Asn Val Asp Leu Leu Pro Arg Gly Ala Pro Glu Arg Gln 
145                 150                 155                 160 


Arg Leu Leu Pro Ala Ala Leu Ala Cys Trp Gly Val Arg Gly Ser Leu 
                165                 170                 175     


Leu Ser Glu Ala Asp Val Arg Ala Leu Gly Gly Leu Ala Cys Asp Leu 
            180                 185                 190         


Pro Gly Arg Phe Val Ala Glu Ser Ala Glu Val Leu Leu Pro Arg Leu 
        195                 200                 205             


Val Ser Cys Pro Gly Pro Leu Asp Gln Asp Gln Gln Glu Ala Ala Arg 
    210                 215                 220                 


Ala Ala Leu Gln Gly Gly Gly Pro Pro Tyr Gly Pro Pro Ser Thr Trp 
225                 230                 235                 240 


Ser Val Ser Thr Met Asp Ala Leu Arg Gly Leu Leu Pro Val Leu Gly 
                245                 250                 255     


Gln Pro Ile Ile Arg Ser Ile Pro Gln Gly Ile Val Ala Ala Trp Arg 
            260                 265                 270         


Gln Arg Ser Ser Arg Asp Pro Ser Trp Arg Gln Pro Glu Arg Thr Ile 
        275                 280                 285             


Leu Arg Pro Arg Phe Arg Arg Glu Val Glu Lys Thr Ala Cys Pro Ser 
    290                 295                 300                 


Gly Lys Lys Ala Arg Glu Ile Asp Glu Ser Leu Ile Phe Tyr Lys Lys 
305                 310                 315                 320 


Trp Glu Leu Glu Ala Cys Val Asp Ala Ala Leu Leu Ala Thr Gln Met 
                325                 330                 335     


Asp Arg Val Asn Ala Ile Pro Phe Thr Tyr Glu Gln Leu Asp Val Leu 
            340                 345                 350         


Lys His Lys Leu Asp Glu Leu Tyr Pro Gln Gly Tyr Pro Glu Ser Val 
        355                 360                 365             


Ile Gln His Leu Gly Tyr Leu Phe Leu Lys Met Ser Pro Glu Asp Ile 
    370                 375                 380                 


Arg Lys Trp Asn Val Thr Ser Leu Glu Thr Leu Lys Ala Leu Leu Glu 
385                 390                 395                 400 


Val Asn Lys Gly His Glu Met Ser Pro Gln Val Ala Thr Leu Ile Asp 
                405                 410                 415     


Arg Phe Val Lys Gly Arg Gly Gln Leu Asp Lys Asp Thr Leu Asp Thr 
            420                 425                 430         


Leu Thr Ala Phe Tyr Pro Gly Tyr Leu Cys Ser Leu Ser Pro Glu Glu 
        435                 440                 445             


Leu Ser Ser Val Pro Pro Ser Ser Ile Trp Ala Val Arg Pro Gln Asp 
    450                 455                 460                 


Leu Asp Thr Cys Asp Pro Arg Gln Leu Asp Val Leu Tyr Pro Lys Ala 
465                 470                 475                 480 


Arg Leu Ala Phe Gln Asn Met Asn Gly Ser Glu Tyr Phe Val Lys Ile 
                485                 490                 495     


Gln Ser Phe Leu Gly Gly Ala Pro Thr Glu Asp Leu Lys Ala Leu Ser 
            500                 505                 510         


Gln Gln Asn Val Ser Met Asp Leu Ala Thr Phe Met Lys Leu Arg Thr 
        515                 520                 525             


Asp Ala Val Leu Pro Leu Thr Val Ala Glu Val Gln Lys Leu Leu Gly 
    530                 535                 540                 


Pro His Val Glu Gly Leu Lys Ala Glu Glu Arg His Arg Pro Val Arg 
545                 550                 555                 560 


Asp Trp Ile Leu Arg Gln Arg Gln Asp Asp Leu Asp Thr Leu Gly Leu 
                565                 570                 575     


Gly Leu Gln Gly Gly Ile Pro Asn Gly Tyr Leu Val Leu Asp Leu Ser 
            580                 585                 590         


Val Gln Glu Ala Leu Ser Gly Thr Pro Cys Leu Leu Gly Pro Gly Pro 
        595                 600                 605             


Val Leu Thr Val Leu Ala Leu Leu Leu Ala Ser Thr Leu Ala 
    610                 615                 620         


<210>  3
<211>  20
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  DNA/RNA Hybrid

<400>  3
ggccggacac cuccgcacac                                                   20


<210>  4
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  DNA/RNA Hybrid

<400>  4
ctctgctcca cctcagatct ga                                                22


<210>  5
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  DNA/RNA Hybrid

<400>  5
cacacagcgt catatcagac ttg                                               23


<210>  6
<211>  213
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  6

Asp Ile Glu Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Asn Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu 
65                  70                  75                  80  


Asp Asp Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Lys His Pro Leu Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         


Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             


Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 


Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 


Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         


Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             


Asn Arg Gly Glu Cys 
    210             


<210>  7
<211>  449
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  7

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Thr Pro Tyr Asn Gly Ala Ser Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Arg Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Asp Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Tyr Asp Gly Arg Gly Phe Asp Tyr Trp Gly Ser Gly 
            100                 105                 110         


Thr Pro Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                325                 330                 335     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            420                 425                 430         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             


Lys 
    


