                         SEQUENCE LISTING

<110>  CELGENE CORPORATION
       ACCELERON PHARMA INC.
 
<120>  TREATMENT OF ANEMIA DUE TO VERY LOW, LOW, OR INTERMEDIATE RISK 
       MYELODYSPLASTIC SYNDROMES IN SUBJECTS WITH RING SIDEROBLASTS 
       USING ACTIVIN-ACTRII LIGAND TRAPS

<130>  14247-343-228

<140>  TBD
<141>  On Even Date Herewith

<150>  US 62/753,379
<151>  2018-10-31

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Processed ActRIIB-Fc fusion protein with the N-terminal 6 amino 
       acids of the EC domain deleted and the C-terminal 3 amino acids 
       of the EC domain deleted and with an L79D mutation (Luspatercept)

<400>  1

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Arg Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Asp Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr Gly Gly Gly Thr His 
            100                 105                 110         


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
        115                 120                 125             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    130                 135                 140                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
145                 150                 155                 160 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                165                 170                 175     


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
            180                 185                 190         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        195                 200                 205             


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
    210                 215                 220                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
225                 230                 235                 240 


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                245                 250                 255     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            260                 265                 270         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        275                 280                 285             


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
    290                 295                 300                 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
305                 310                 315                 320 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330                 335 


<210>  2
<211>  688
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Extracellular domain of human ActRIIA fused to a human Fc domain 
       (Sotatercept)

<400>  2

Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe Phe Asn Ala Asn 
1               5                   10                  15      


Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu Pro Cys Tyr Gly 
            20                  25                  30          


Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp Lys Asn Ile Ser 
        35                  40                  45              


Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu Asp Asp Ile Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp Ser Pro Glu Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu Lys Phe Ser Tyr 
                85                  90                  95      


Phe Pro Glu Met Glu Val Thr Gln Pro Thr Ser Asn Pro Val Thr Pro 
            100                 105                 110         


Lys Pro Pro Thr Gly Gly Gly Thr His Thr Cys Pro Pro Cys Pro Ala 
        115                 120                 125             


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    130                 135                 140                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
145                 150                 155                 160 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
                165                 170                 175     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            180                 185                 190         


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        195                 200                 205             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
    210                 215                 220                 


Leu Pro Val Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
225                 230                 235                 240 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
                245                 250                 255     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            260                 265                 270         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        275                 280                 285             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    290                 295                 300                 


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
305                 310                 315                 320 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                325                 330                 335     


Ser Leu Ser Leu Ser Pro Gly Lys Ile Leu Gly Arg Ser Glu Thr Gln 
            340                 345                 350         


Glu Cys Leu Phe Phe Asn Ala Asn Trp Glu Lys Asp Arg Thr Asn Gln 
        355                 360                 365             


Thr Gly Val Glu Pro Cys Tyr Gly Asp Lys Asp Lys Arg Arg His Cys 
    370                 375                 380                 


Phe Ala Thr Trp Lys Asn Ile Ser Gly Ser Ile Glu Ile Val Lys Gln 
385                 390                 395                 400 


Gly Cys Trp Leu Asp Asp Ile Asn Cys Tyr Asp Arg Thr Asp Cys Val 
                405                 410                 415     


Glu Lys Lys Asp Ser Pro Glu Val Tyr Phe Cys Cys Cys Glu Gly Asn 
            420                 425                 430         


Met Cys Asn Glu Lys Phe Ser Tyr Phe Pro Glu Met Glu Val Thr Gln 
        435                 440                 445             


Pro Thr Ser Asn Pro Val Thr Pro Lys Pro Pro Thr Gly Gly Gly Thr 
    450                 455                 460                 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
465                 470                 475                 480 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
                485                 490                 495     


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
            500                 505                 510         


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
        515                 520                 525             


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
    530                 535                 540                 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
545                 550                 555                 560 


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Val Pro Ile Glu Lys Thr 
                565                 570                 575     


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
            580                 585                 590         


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
        595                 600                 605             


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
    610                 615                 620                 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
625                 630                 635                 640 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
                645                 650                 655     


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
            660                 665                 670         


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        675                 680                 685             


<210>  3
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human ActRIIB soluble (extracellular), processed polypeptide 
       sequence

<400>  3

Ser Gly Arg Gly Glu Ala Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala 
1               5                   10                  15      


Asn Trp Glu Leu Glu Arg Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu 
            20                  25                  30          


Gly Glu Gln Asp Lys Arg Leu His Cys Tyr Ala Ser Trp Ala Asn Ser 
        35                  40                  45              


Ser Gly Thr Ile Glu Leu Val Lys Lys Gly Cys Trp Leu Asp Asp Phe 
    50                  55                  60                  


Asn Cys Tyr Asp Arg Gln Glu Cys Val Ala Thr Glu Glu Asn Pro Gln 
65                  70                  75                  80  


Val Tyr Phe Cys Cys Cys Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr 
                85                  90                  95      


His Leu Pro Glu Ala Gly Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro 
            100                 105                 110         


Thr Ala Pro Thr 
        115     


<210>  4
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human ActRIIA soluble (extracellular), processed polypeptide 
       sequence

<400>  4

Ile Leu Gly Arg Ser Glu Thr Gln Glu Cys Leu Phe Phe Asn Ala Asn 
1               5                   10                  15      


Trp Glu Lys Asp Arg Thr Asn Gln Thr Gly Val Glu Pro Cys Tyr Gly 
            20                  25                  30          


Asp Lys Asp Lys Arg Arg His Cys Phe Ala Thr Trp Lys Asn Ile Ser 
        35                  40                  45              


Gly Ser Ile Glu Ile Val Lys Gln Gly Cys Trp Leu Asp Asp Ile Asn 
    50                  55                  60                  


Cys Tyr Asp Arg Thr Asp Cys Val Glu Lys Lys Asp Ser Pro Glu Val 
65                  70                  75                  80  


Tyr Phe Cys Cys Cys Glu Gly Asn Met Cys Asn Glu Lys Phe Ser Tyr 
                85                  90                  95      


Phe Pro Glu Met Glu Val Thr Gln Pro Thr Ser Asn Pro Val Thr Pro 
            100                 105                 110         


Lys Pro Pro 
        115 


<210>  5
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A fragment of human ActRIIB soluble (extracellular), processed 
       polypeptide sequence (amino acids 7-113 of SEQ ID NO: 3)

<400>  5

Glu Thr Arg Glu Cys Ile Tyr Tyr Asn Ala Asn Trp Glu Leu Glu Arg 
1               5                   10                  15      


Thr Asn Gln Ser Gly Leu Glu Arg Cys Glu Gly Glu Gln Asp Lys Arg 
            20                  25                  30          


Leu His Cys Tyr Ala Ser Trp Ala Asn Ser Ser Gly Thr Ile Glu Leu 
        35                  40                  45              


Val Lys Lys Gly Cys Trp Leu Asp Asp Phe Asn Cys Tyr Asp Arg Gln 
    50                  55                  60                  


Glu Cys Val Ala Thr Glu Glu Asn Pro Gln Val Tyr Phe Cys Cys Cys 
65                  70                  75                  80  


Glu Gly Asn Phe Cys Asn Glu Arg Phe Thr His Leu Pro Glu Ala Gly 
                85                  90                  95      


Gly Pro Glu Val Thr Tyr Glu Pro Pro Pro Thr 
            100                 105         


