                         SEQUENCE LISTING

<110>  BASSI, ROBERTO
       DALL'OSTO, LUCA
       BENEDETTI, MANUEL
 
<120>  TRANSGENIC MICROALGAE FOR THE PRODUCTION OF PLANT CELL WALL 
       DEGRADING ENZYMES WITH THERMOSTABLE CELLULOLYTIC ACTIVITY

<130>  P1610PC

<150>  102018000009867
<151>  2018-10-29

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  267
<212>  PRT
<213>  Thermotoga neapolitana


<220>
<221>  MISC_FEATURE
<222>  (258)..(267)
<223>  HA tag

<400>  1

Met Ala Glu Val Val Leu Thr Asp Ile Gly Ala Thr Asp Ile Thr Phe 
1               5                   10                  15      


Lys Gly Phe Pro Val Thr Met Glu Leu Asn Phe Trp Asn Val Lys Ser 
            20                  25                  30          


Tyr Glu Gly Glu Thr Trp Leu Lys Phe Asp Gly Glu Lys Val Gln Phe 
        35                  40                  45              


Tyr Ala Asp Ile Tyr Asn Ile Val Leu Gln Asn Pro Asp Ser Trp Val 
    50                  55                  60                  


His Gly Tyr Pro Glu Ile Tyr Tyr Gly Tyr Lys Pro Trp Ala Ala His 
65                  70                  75                  80  


Asn Ser Gly Thr Glu Ile Leu Pro Val Lys Val Lys Asp Leu Pro Asp 
                85                  90                  95      


Phe Tyr Val Thr Leu Asp Tyr Ser Ile Trp Tyr Glu Asn Asp Leu Pro 
            100                 105                 110         


Ile Asn Leu Ala Met Glu Thr Trp Ile Thr Arg Lys Pro Asp Gln Thr 
        115                 120                 125             


Ser Val Ser Ser Gly Asp Val Glu Ile Met Val Trp Phe Tyr Asn Asn 
    130                 135                 140                 


Ile Leu Met Pro Gly Gly Gln Lys Val Asp Glu Phe Thr Thr Thr Ile 
145                 150                 155                 160 


Glu Ile Asn Gly Ser Pro Val Glu Thr Lys Trp Asp Val Tyr Phe Ala 
                165                 170                 175     


Pro Trp Gly Trp Asp Tyr Leu Ala Phe Arg Leu Thr Thr Pro Met Lys 
            180                 185                 190         


Asp Gly Arg Val Lys Phe Asn Val Lys Asp Phe Val Glu Lys Ala Ala 
        195                 200                 205             


Glu Val Ile Lys Lys His Ser Thr Arg Val Glu Asn Phe Asp Glu Met 
    210                 215                 220                 


Tyr Phe Cys Val Trp Glu Ile Gly Thr Glu Phe Gly Asp Pro Asn Thr 
225                 230                 235                 240 


Thr Ala Ala Lys Phe Gly Trp Thr Phe Lys Asp Phe Ser Val Glu Ile 
                245                 250                 255     


Gly Glu Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
            260                 265         


<210>  2
<211>  812
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon usage optimized sequence encoding CelB di Thermotoga 
       neapolitana with HA tag


<220>
<221>  misc_feature
<222>  (777)..(803)
<223>  HA tag

<400>  2
ccatggctga agttgtttta acagatattg gtgctacaga tattacattt aaaggttttc       60

cagttacaat ggaattaaat ttttggaatg ttaaatcata tgaaggtgaa acatggttaa      120

aatttgatgg tgaaaaagtt caattttatg ctgatattta taatattgtt ttacaaaatc      180

cagattcatg ggttcatggt tatccagaaa tttattatgg ttataaacct tgggctgctc      240

ataattcagg tacagaaatt ttaccagtta aagttaaaga tttaccagat ttttatgtta      300

cattagatta ttcaatttgg tatgaaaatg atttaccaat taatttagct atggaaacat      360

ggattacacg taaaccagat caaacatcag tttcatcagg tgatgttgaa attatggttt      420

ggttttataa taatatttta atgccaggtg gtcaaaaagt tgatgaattt acaacaacaa      480

ttgaaattaa tggttcacca gttgaaacaa aatgggatgt ttattttgct ccttggggtt      540

gggattattt agcttttcgt ttaacaacac caatgaaaga tggtcgtgtt aaatttaatg      600

ttaaagattt tgttgaaaaa gctgctgaag ttattaaaaa acattcaaca cgtgttgaaa      660

attttgatga aatgtatttt tgtgtttggg aaattggtac agaatttggt gatccaaata      720

caacagctgc taaatttggt tggacattta aagatttttc agttgaaatt ggtgaatatc      780

catatgatgt tccagattat gcttgagcat gc                                    812


<210>  3
<211>  676
<212>  PRT
<213>  Caldicellulosiruptor saccharolyticus


<220>
<221>  MISC_FEATURE
<222>  (668)..(776)
<223>  HA tag

<400>  3

Met Gly Val Thr Thr Ser Ser Pro Thr Pro Thr Pro Thr Pro Thr Val 
1               5                   10                  15      


Thr Val Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro Thr Val Thr Ala 
            20                  25                  30          


Thr Pro Thr Pro Thr Pro Thr Pro Val Ser Thr Pro Ala Thr Gly Gly 
        35                  40                  45              


Gln Ile Lys Val Leu Tyr Ala Asn Lys Glu Thr Asn Ser Thr Thr Asn 
    50                  55                  60                  


Thr Ile Arg Pro Trp Leu Lys Val Val Asn Ser Gly Ser Ser Ser Ile 
65                  70                  75                  80  


Asp Leu Ser Arg Val Thr Ile Arg Tyr Trp Tyr Thr Val Asp Gly Glu 
                85                  90                  95      


Arg Ala Gln Ser Ala Val Ser Asp Trp Ala Gln Ile Gly Ala Ser Asn 
            100                 105                 110         


Val Thr Phe Lys Phe Val Lys Leu Ser Ser Ser Val Ser Gly Ala Asp 
        115                 120                 125             


Tyr Tyr Leu Glu Ile Gly Phe Lys Ser Gly Ala Gly Gln Leu Gln Pro 
    130                 135                 140                 


Gly Lys Asp Thr Gly Glu Ile Gln Ile Arg Phe Asn Lys Ser Asp Trp 
145                 150                 155                 160 


Ser Asn Tyr Asn Gln Gly Asn Asp Trp Ser Trp Leu Gln Ser Met Thr 
                165                 170                 175     


Ser Tyr Gly Glu Asn Glu Lys Val Thr Ala Tyr Ile Asp Gly Val Leu 
            180                 185                 190         


Val Trp Gly Gln Glu Pro Ser Gly Ala Thr Pro Ala Pro Thr Met Thr 
        195                 200                 205             


Val Ala Pro Thr Ala Thr Pro Thr Pro Thr Leu Ser Pro Thr Val Thr 
    210                 215                 220                 


Pro Thr Pro Ala Pro Thr Gln Thr Ala Ile Pro Thr Pro Thr Leu Thr 
225                 230                 235                 240 


Pro Asn Pro Thr Pro Thr Ser Ser Ile Pro Asp Asp Thr Asn Asp Asp 
                245                 250                 255     


Trp Leu Tyr Val Ser Gly Asn Lys Ile Val Asp Lys Asp Gly Arg Pro 
            260                 265                 270         


Val Trp Leu Thr Gly Ile Asn Trp Phe Gly Tyr Asn Thr Gly Thr Asn 
        275                 280                 285             


Val Phe Asp Gly Val Trp Ser Cys Asn Leu Lys Asp Thr Leu Ala Glu 
    290                 295                 300                 


Ile Ala Asn Arg Gly Phe Asn Leu Leu Arg Val Pro Ile Ser Ala Glu 
305                 310                 315                 320 


Leu Ile Leu Asn Trp Ser Gln Gly Ile Tyr Pro Lys Pro Asn Ile Asn 
                325                 330                 335     


Tyr Tyr Val Asn Pro Glu Leu Glu Gly Lys Asn Ser Leu Glu Val Phe 
            340                 345                 350         


Asp Ile Val Val Gln Thr Cys Lys Glu Val Gly Leu Lys Ile Met Leu 
        355                 360                 365             


Asp Ile His Ser Ile Lys Thr Asp Ala Met Gly His Ile Tyr Pro Val 
    370                 375                 380                 


Trp Tyr Asp Glu Lys Phe Thr Pro Glu Asp Phe Tyr Lys Ala Cys Glu 
385                 390                 395                 400 


Trp Ile Thr Asn Arg Tyr Lys Asn Asp Asp Thr Ile Ile Ala Phe Asp 
                405                 410                 415     


Leu Lys Asn Glu Pro His Gly Lys Pro Trp Gln Asp Thr Thr Phe Ala 
            420                 425                 430         


Lys Trp Asp Asn Ser Thr Asp Ile Asn Asn Trp Lys Tyr Ala Ala Glu 
        435                 440                 445             


Thr Cys Ala Lys Arg Ile Leu Asn Ile Asn Pro Asn Leu Leu Ile Val 
    450                 455                 460                 


Ile Glu Gly Ile Glu Ala Tyr Pro Lys Asp Asp Val Thr Trp Thr Ser 
465                 470                 475                 480 


Lys Ser Ser Ser Asp Tyr Tyr Ser Thr Trp Trp Gly Gly Asn Leu Arg 
                485                 490                 495     


Gly Val Arg Lys Tyr Pro Ile Asn Leu Gly Lys Tyr Gln Asn Lys Val 
            500                 505                 510         


Val Tyr Ser Pro His Asp Tyr Gly Pro Ser Val Tyr Gln Gln Pro Trp 
        515                 520                 525             


Phe Tyr Pro Gly Phe Thr Lys Glu Ser Leu Leu Gln Asp Cys Trp Arg 
    530                 535                 540                 


Pro Asn Trp Ala Tyr Ile Met Glu Glu Asn Ile Ala Pro Leu Leu Ile 
545                 550                 555                 560 


Gly Glu Trp Gly Gly His Leu Asp Gly Ala Asp Asn Glu Lys Trp Met 
                565                 570                 575     


Lys Tyr Leu Arg Asp Tyr Ile Ile Glu Asn His Ile His His Thr Phe 
            580                 585                 590         


Trp Cys Phe Asn Ala Asn Ser Gly Asp Thr Gly Gly Leu Val Gly Tyr 
        595                 600                 605             


Asp Phe Thr Thr Trp Asp Glu Lys Lys Tyr Ser Phe Leu Lys Pro Ala 
    610                 615                 620                 


Leu Trp Gln Asp Ser Gln Gly Arg Phe Val Gly Leu Asp His Lys Arg 
625                 630                 635                 640 


Pro Leu Gly Thr Asn Gly Lys Asn Ile Asn Ile Thr Thr Tyr Tyr Asn 
                645                 650                 655     


Asn Asn Glu Pro Glu Pro Val Pro Ala Ser Lys Tyr Pro Tyr Asp Val 
            660                 665                 670         


Pro Asp Tyr Ala 
        675     


<210>  4
<211>  2039
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon-optimized sequence of CBM3GH5 from Caldicellulosiruptor 
       saccharolyticus with HA tag


<220>
<221>  misc_feature
<222>  (2004)..(2030)
<223>  HA tag

<400>  4
ccatgggtgt tacaacatca tcaccaacac caacaccaac accaacagtt acagttacac       60

caacaccaac accaacacca acaccaacag ttacagctac accaacacca acaccaacac      120

cagtttcaac accagctaca ggtggtcaaa ttaaagtttt atatgctaat aaagaaacaa      180

attcaacaac aaatacaatt cgtccttggt taaaagttgt taattcaggt tcatcatcaa      240

ttgatttatc acgtgttaca attcgttatt ggtatacagt tgatggtgaa cgtgctcaat      300

cagctgtttc agattgggct caaattggtg cttcaaatgt tacatttaaa tttgttaaat      360

tatcatcatc agtttcaggt gctgattatt atttagaaat tggttttaaa tcaggtgctg      420

gtcaattaca accaggtaaa gatacaggtg aaattcaaat tcgttttaat aaatcagatt      480

ggtcaaatta taatcaaggt aatgattggt catggttaca atcaatgaca tcatatggtg      540

aaaatgaaaa agttacagct tatattgatg gtgttttagt ttggggtcaa gaaccatcag      600

gtgctacacc agctccaaca atgacagttg ctccaacagc tacaccaaca ccaacattat      660

caccaacagt tacaccaaca ccagctccaa cacaaacagc tattccaaca ccaacattaa      720

caccaaatcc aacaccaaca tcatcaattc cagatgatac aaatgatgat tggttatatg      780

tttcaggtaa taaaattgtt gataaagatg gtcgtccagt ttggttaaca ggtattaatt      840

ggtttggtta taatacaggt acaaatgttt ttgatggtgt ttggtcatgt aatttaaaag      900

atacattagc tgaaattgct aatcgtggtt ttaatttatt acgtgttcca atttcagctg      960

aattaatttt aaattggtca caaggtattt atccaaaacc aaatattaat tattatgtta     1020

atccagaatt agaaggtaaa aattcattag aagtttttga tattgttgtt caaacatgta     1080

aagaagttgg tttaaaaatt atgttagata ttcattcaat taaaacagat gctatgggtc     1140

atatttatcc agtttggtat gatgaaaaat ttacaccaga agatttttat aaagcttgtg     1200

aatggattac aaatcgttat aaaaatgatg atacaattat tgcttttgat ttaaaaaatg     1260

aaccacatgg taaaccttgg caagatacaa catttgctaa atgggataat tcaacagata     1320

ttaataattg gaaatatgct gctgaaacat gtgctaaacg tattttaaat attaatccaa     1380

atttattaat tgttattgaa ggtattgaag cttatccaaa agatgatgtt acatggacat     1440

caaaatcatc atcagattat tattcaacat ggtggggtgg taatttacgt ggtgttcgta     1500

aatatccaat taatttaggt aaatatcaaa ataaagttgt ttattcacca catgattatg     1560

gtccatcagt ttatcaacaa ccttggtttt atccaggttt tacaaaagaa tcattattac     1620

aagattgttg gcgtccaaat tgggcttata ttatggaaga aaatattgct ccattattaa     1680

ttggtgaatg gggtggtcat ttagatggtg ctgataatga aaaatggatg aaatatttac     1740

gtgattatat tattgaaaat catattcatc atacattttg gtgttttaat gctaattcag     1800

gtgatacagg tggtttagtt ggttatgatt ttacaacatg ggatgaaaaa aaatattcat     1860

ttttaaaacc agctttatgg caagattcac aaggtcgttt tgttggttta gatcataaac     1920

gtccattagg tacaaatggt aaaaatatta atattacaac atattataat aataatgaac     1980

cagaaccagt tccagcttca aaatatccat atgatgttcc agattatgct tgagcatgc      2039


<210>  5
<211>  482
<212>  PRT
<213>  Pyrococcus furiosus


<220>
<221>  MISC_FEATURE
<222>  (474)..(482)
<223>  HA tag

<400>  5

Met Ala Lys Phe Pro Lys Asn Phe Met Phe Gly Tyr Ser Trp Ser Gly 
1               5                   10                  15      


Phe Gln Phe Glu Met Gly Leu Pro Gly Ser Glu Val Glu Ser Asp Trp 
            20                  25                  30          


Trp Val Trp Val His Asp Lys Glu Asn Ile Ala Ser Gly Leu Val Ser 
        35                  40                  45              


Gly Asp Leu Pro Glu Asn Gly Pro Ala Tyr Trp His Leu Tyr Lys Gln 
    50                  55                  60                  


Asp His Asp Ile Ala Glu Lys Leu Gly Met Asp Cys Ile Arg Gly Gly 
65                  70                  75                  80  


Ile Glu Trp Ala Arg Ile Phe Pro Lys Pro Thr Phe Asp Val Lys Val 
                85                  90                  95      


Asp Val Glu Lys Asp Glu Glu Gly Asn Ile Ile Ser Val Asp Val Pro 
            100                 105                 110         


Glu Ser Thr Ile Lys Glu Leu Glu Lys Ile Ala Asn Met Glu Ala Leu 
        115                 120                 125             


Glu His Tyr Arg Lys Ile Tyr Ser Asp Trp Lys Glu Arg Gly Lys Thr 
    130                 135                 140                 


Phe Ile Leu Asn Leu Tyr His Trp Pro Leu Pro Leu Trp Ile His Asp 
145                 150                 155                 160 


Pro Ile Ala Val Arg Lys Leu Gly Pro Asp Arg Ala Pro Ala Gly Trp 
                165                 170                 175     


Leu Asp Glu Lys Thr Val Val Glu Phe Val Lys Phe Ala Ala Phe Val 
            180                 185                 190         


Ala Tyr His Leu Asp Asp Leu Val Asp Met Trp Ser Thr Met Asn Glu 
        195                 200                 205             


Pro Asn Val Val Tyr Asn Gln Gly Tyr Ile Asn Leu Arg Ser Gly Phe 
    210                 215                 220                 


Pro Pro Gly Tyr Leu Ser Phe Glu Ala Ala Glu Lys Ala Lys Phe Asn 
225                 230                 235                 240 


Leu Ile Gln Ala His Ile Gly Ala Tyr Asp Ala Ile Lys Glu Tyr Ser 
                245                 250                 255     


Glu Lys Ser Val Gly Val Ile Tyr Ala Phe Ala Trp His Asp Pro Leu 
            260                 265                 270         


Ala Glu Glu Tyr Lys Asp Glu Val Glu Glu Ile Arg Lys Lys Asp Tyr 
        275                 280                 285             


Glu Phe Val Thr Ile Leu His Ser Lys Gly Lys Leu Asp Trp Ile Gly 
    290                 295                 300                 


Val Asn Tyr Tyr Ser Arg Leu Val Tyr Gly Ala Lys Asp Gly His Leu 
305                 310                 315                 320 


Val Pro Leu Pro Gly Tyr Gly Phe Met Ser Glu Arg Gly Gly Phe Ala 
                325                 330                 335     


Lys Ser Gly Arg Pro Ala Ser Asp Phe Gly Trp Glu Met Tyr Pro Glu 
            340                 345                 350         


Gly Leu Glu Asn Leu Leu Lys Tyr Leu Asn Asn Ala Tyr Glu Leu Pro 
        355                 360                 365             


Met Ile Ile Thr Glu Asn Gly Met Ala Asp Ala Ala Asp Arg Tyr Arg 
    370                 375                 380                 


Pro His Tyr Leu Val Ser His Leu Lys Ala Val Tyr Asn Ala Met Lys 
385                 390                 395                 400 


Glu Gly Ala Asp Val Arg Gly Tyr Leu His Trp Ser Leu Thr Asp Asn 
                405                 410                 415     


Tyr Glu Trp Ala Gln Gly Phe Arg Met Arg Phe Gly Leu Val Tyr Val 
            420                 425                 430         


Asp Phe Glu Thr Lys Lys Arg Tyr Leu Arg Pro Ser Ala Leu Val Phe 
        435                 440                 445             


Arg Glu Ile Ala Thr Gln Lys Glu Ile Pro Glu Glu Leu Ala His Leu 
    450                 455                 460                 


Ala Asp Leu Lys Phe Val Thr Arg Lys Tyr Pro Tyr Asp Val Pro Asp 
465                 470                 475                 480 


Tyr Ala 
        


<210>  6
<211>  1457
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon optimized seqeunce encoding for beta glucosidase from 
       Pyrococcus furiosus with HA tag


<220>
<221>  misc_feature
<222>  (1443)..(1457)
<223>  HA tag

<400>  6
ccatggctaa atttccaaaa aattttatgt ttggttattc atggtcaggt tttcaatttg       60

aaatgggttt accaggttca gaagttgaat cagattggtg ggtttgggtt catgataaag      120

aaaatattgc ttcaggttta gtttcaggtg atttaccaga aaatggtcca gcttattggc      180

atttatataa acaagatcat gatattgctg aaaaattagg tatggattgt attcgtggtg      240

gtattgaatg ggctcgtatt tttccaaaac caacatttga tgttaaagtt gatgttgaaa      300

aagatgaaga aggtaatatt atttcagttg atgttccaga atcaacaatt aaagaattag      360

aaaaaattgc taatatggaa gcattagaac attatcgtaa aatttattca gattggaaag      420

aacgtggtaa aacatttatt ttaaatttat atcattggcc attaccatta tggattcatg      480

atccaattgc tgttcgtaaa ttaggtccag atcgtgctcc agctggttgg ttagatgaaa      540

aaacagttgt tgaatttgtt aaatttgctg cttttgttgc ttatcattta gatgatttag      600

ttgatatgtg gtcaacaatg aatgaaccaa atgttgttta taatcaaggt tatattaatt      660

tacgttcagg ttttccacca ggttatttat catttgaagc tgctgaaaaa gctaaattta      720

atttaattca agctcatatt ggtgcttatg atgctattaa agaatattca gaaaaatcag      780

ttggtgttat ttatgctttt gcttggcatg atccattagc tgaagaatat aaagatgaag      840

ttgaagaaat tcgtaaaaaa gattatgaat ttgttacaat tttacattca aaaggtaaat      900

tagattggat tggtgttaat tattattcac gtttagttta tggtgctaaa gatggtcatt      960

tagttccatt accaggttat ggttttatgt cagaacgtgg tggttttgct aaatcaggtc     1020

gtccagcttc agattttggt tgggaaatgt atccagaagg tttagaaaat ttattaaaat     1080

atttaaataa tgcttatgaa ttaccaatga ttattacaga aaatggtatg gctgatgctg     1140

ctgatcgtta tcgtccacat tatttagttt cacatttaaa agctgtttat aatgctatga     1200

aagaaggtgc tgatgttcgt ggttatttac attggtcatt aacagataat tatgaatggg     1260

ctcaaggttt tcgtatgcgt tttggtttag tttatgttga ttttgaaaca aaaaaacgtt     1320

atttacgtcc atcagcttta gtttttcgtg aaattgctac acaaaaagaa attccagaag     1380

aattagctca tttagctgat ttaaaatttg ttacacgtaa atatccatat gatgttccag     1440

attatgctta agcatgc                                                    1457


<210>  7
<211>  1036
<212>  PRT
<213>  Thermotoga neapolitana


<220>
<221>  MISC_FEATURE
<222>  (1028)..(1036)
<223>  HA tag

<400>  7

Met Ala Thr Gly Ala Leu Gly Phe Gly Gly Lys Gly Val Ser Pro Phe 
1               5                   10                  15      


Glu Thr Val Leu Val Leu Ser Phe Glu Gly Asn Thr Asp Gly Ala Ser 
            20                  25                  30          


Pro Phe Gly Lys Asp Val Val Val Thr Ala Ser Gln Asp Val Ala Ala 
        35                  40                  45              


Asp Gly Glu Tyr Ser Leu Lys Val Glu Asn Arg Thr Ser Val Trp Asp 
    50                  55                  60                  


Gly Val Glu Ile Asp Leu Thr Gly Lys Val Asn Thr Gly Thr Asp Tyr 
65                  70                  75                  80  


Leu Leu Ser Phe His Val Tyr Gln Thr Ser Asp Ser Pro Gln Leu Phe 
                85                  90                  95      


Ser Val Leu Ala Arg Thr Glu Asp Glu Lys Gly Glu Arg Tyr Lys Ile 
            100                 105                 110         


Leu Ala Asp Lys Val Val Val Pro Asn Tyr Trp Lys Glu Ile Leu Val 
        115                 120                 125             


Pro Phe Ser Pro Thr Phe Glu Gly Thr Pro Ala Lys Phe Ser Leu Ile 
    130                 135                 140                 


Ile Thr Ser Pro Lys Lys Thr Asp Phe Val Phe Tyr Val Asp Asn Val 
145                 150                 155                 160 


Gln Val Leu Thr Pro Lys Glu Ala Gly Pro Lys Val Val Tyr Glu Thr 
                165                 170                 175     


Ser Phe Glu Lys Gly Ile Gly Asp Trp Gln Pro Arg Gly Ser Asp Val 
            180                 185                 190         


Lys Ile Ser Ile Ser Pro Lys Val Ala His Ser Gly Lys Lys Ser Leu 
        195                 200                 205             


Phe Val Ser Asn Arg Gln Lys Gly Trp His Gly Ala Gln Ile Ser Leu 
    210                 215                 220                 


Lys Gly Ile Leu Lys Thr Gly Lys Thr Tyr Ala Phe Glu Ala Trp Val 
225                 230                 235                 240 


Tyr Gln Glu Ser Gly Gln Asp Gln Thr Ile Ile Met Thr Met Gln Arg 
                245                 250                 255     


Lys Tyr Ser Ser Asp Ser Ser Thr Lys Tyr Glu Trp Ile Lys Ala Ala 
            260                 265                 270         


Thr Val Pro Ser Gly Gln Trp Val Gln Leu Ser Gly Thr Tyr Thr Ile 
        275                 280                 285             


Pro Ala Gly Val Thr Val Glu Asp Leu Thr Leu Tyr Phe Glu Ser Gln 
    290                 295                 300                 


Asn Pro Thr Leu Glu Phe Tyr Val Asp Asp Val Lys Val Val Asp Thr 
305                 310                 315                 320 


Thr Ser Ala Glu Ile Lys Leu Glu Met Asn Pro Glu Glu Glu Ile Pro 
                325                 330                 335     


Ala Leu Lys Asp Val Leu Lys Asp Tyr Phe Arg Val Gly Val Ala Leu 
            340                 345                 350         


Pro Ser Lys Val Phe Ile Asn Gln Lys Asp Ile Ala Leu Ile Ser Lys 
        355                 360                 365             


His Phe Asn Ser Ile Thr Ala Glu Asn Glu Met Lys Pro Asp Ser Leu 
    370                 375                 380                 


Leu Ala Gly Ile Glu Asn Gly Lys Leu Lys Phe Arg Phe Glu Thr Ala 
385                 390                 395                 400 


Asp Lys Tyr Ile Glu Phe Ala Gln Gln Asn Gly Met Val Val Arg Gly 
                405                 410                 415     


His Thr Leu Val Trp His Asn Gln Thr Pro Glu Trp Phe Phe Lys Asp 
            420                 425                 430         


Glu Asn Gly Asn Leu Leu Ser Lys Glu Glu Met Thr Glu Arg Leu Arg 
        435                 440                 445             


Glu Tyr Ile His Thr Val Val Gly His Phe Lys Gly Lys Val Tyr Ala 
    450                 455                 460                 


Trp Asp Val Val Asn Glu Ala Val Asp Pro Asn Gln Pro Asp Gly Leu 
465                 470                 475                 480 


Arg Arg Ser Thr Trp Tyr Gln Ile Met Gly Pro Asp Tyr Ile Glu Leu 
                485                 490                 495     


Ala Phe Lys Phe Ala Arg Glu Ala Asp Pro Asn Ala Lys Leu Phe Tyr 
            500                 505                 510         


Asn Asp Tyr Asn Thr Phe Glu Pro Lys Lys Arg Asp Ile Ile Tyr Asn 
        515                 520                 525             


Leu Val Lys Ser Leu Lys Glu Lys Gly Leu Ile Asp Gly Ile Gly Met 
    530                 535                 540                 


Gln Cys His Ile Ser Leu Ala Thr Asp Ile Arg Gln Ile Glu Glu Ala 
545                 550                 555                 560 


Ile Lys Lys Phe Ser Thr Ile Pro Gly Ile Glu Ile His Ile Thr Glu 
                565                 570                 575     


Leu Asp Ile Ser Val Tyr Arg Asp Ser Thr Ser Asn Tyr Ser Glu Ala 
            580                 585                 590         


Pro Arg Thr Ala Leu Ile Glu Gln Ala His Lys Met Ala Gln Leu Phe 
        595                 600                 605             


Lys Ile Phe Lys Lys Tyr Ser Asn Val Ile Thr Asn Val Thr Phe Trp 
    610                 615                 620                 


Gly Leu Lys Asp Asp Tyr Ser Trp Arg Ala Thr Arg Arg Asn Asp Trp 
625                 630                 635                 640 


Pro Leu Ile Phe Asp Lys Asp Tyr Gln Ala Lys Leu Ala Tyr Trp Ala 
                645                 650                 655     


Ile Val Ala Pro Glu Val Leu Pro Pro Leu Pro Lys Glu Ser Lys Ile 
            660                 665                 670         


Ser Glu Gly Glu Ala Val Val Val Gly Met Met Asp Asp Ser Tyr Met 
        675                 680                 685             


Met Ser Lys Pro Ile Glu Ile Tyr Asp Glu Glu Gly Asn Val Lys Ala 
    690                 695                 700                 


Thr Ile Arg Ala Ile Trp Lys Asp Ser Thr Ile Tyr Val Tyr Gly Glu 
705                 710                 715                 720 


Val Gln Asp Ala Thr Lys Lys Pro Ala Glu Asp Gly Val Ala Ile Phe 
                725                 730                 735     


Ile Asn Pro Asn Asn Glu Arg Thr Pro Tyr Leu Gln Pro Asp Asp Thr 
            740                 745                 750         


Tyr Val Val Leu Trp Thr Asn Trp Lys Ser Glu Val Asn Arg Glu Asp 
        755                 760                 765             


Val Glu Val Lys Lys Phe Val Gly Pro Gly Phe Arg Arg Tyr Ser Phe 
    770                 775                 780                 


Glu Met Ser Ile Thr Ile Pro Gly Val Glu Phe Lys Lys Asp Ser Tyr 
785                 790                 795                 800 


Ile Gly Phe Asp Val Ala Val Ile Asp Asp Gly Lys Trp Tyr Ser Trp 
                805                 810                 815     


Ser Asp Thr Thr Asn Ser Gln Lys Thr Asn Thr Met Asn Tyr Gly Thr 
            820                 825                 830         


Leu Lys Leu Glu Gly Val Met Val Ala Thr Ala Lys Tyr Gly Thr Pro 
        835                 840                 845             


Val Ile Asp Gly Glu Ile Asp Asp Ile Trp Asn Thr Thr Glu Glu Ile 
    850                 855                 860                 


Glu Thr Lys Ser Val Ala Met Gly Ser Leu Glu Lys Asn Ala Thr Ala 
865                 870                 875                 880 


Lys Val Arg Val Leu Trp Asp Glu Glu Asn Leu Tyr Val Leu Ala Ile 
                885                 890                 895     


Val Lys Asp Pro Val Leu Asn Lys Asp Asn Ser Asn Pro Trp Glu Gln 
            900                 905                 910         


Asp Ser Val Glu Ile Phe Ile Asp Glu Asn Asn His Lys Thr Gly Tyr 
        915                 920                 925             


Tyr Glu Asp Asp Asp Ala Gln Phe Arg Val Asn Tyr Met Asn Glu Gln 
    930                 935                 940                 


Ser Phe Gly Thr Gly Ala Ser Ala Ala Arg Phe Lys Thr Ala Val Lys 
945                 950                 955                 960 


Leu Ile Glu Gly Gly Tyr Ile Val Glu Ala Ala Ile Lys Trp Lys Thr 
                965                 970                 975     


Ile Lys Pro Ser Pro Asn Thr Val Ile Gly Phe Asn Val Gln Val Asn 
            980                 985                 990         


Asp Ala Asn Glu Lys Gly Gln Arg  Val Gly Ile Ile Ser  Trp Ser Asp 
        995                 1000                 1005             


Pro Thr  Asn Asn Ser Trp Arg  Asp Pro Ser Lys Phe  Gly Asn Leu 
    1010                 1015                 1020             


Arg Leu  Ile Lys Tyr Pro Tyr  Asp Val Pro Asp Tyr  Ala 
    1025                 1030                 1035     


<210>  8
<211>  3119
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon optimized sequence encoding xylanase XynA from Thermotoga 
       neapolitana with HA tag

<400>  8
ccatggctac aggtgcttta ggttttggtg gtaaaggtgt ttcaccattt gaaacagttt       60

tagttttatc atttgaaggt aatacagatg gtgcttcacc atttggtaaa gatgttgttg      120

ttacagcttc acaagatgtt gctgctgatg gtgaatattc attaaaagtt gaaaatcgta      180

catcagtttg ggatggtgtt gaaattgatt taacaggtaa agttaataca ggtacagatt      240

atttattatc atttcatgtt tatcaaacat cagattcacc acaattattt tcagttttag      300

ctcgtacaga agatgaaaaa ggtgaacgtt ataaaatttt agctgataaa gttgttgttc      360

caaattattg gaaagaaatt ttagttccat tttcaccaac atttgaaggt acaccagcta      420

aattttcatt aattattaca tcaccaaaaa aaacagattt tgttttttat gttgataatg      480

ttcaagtttt aacaccaaaa gaagctggtc caaaagttgt ttatgaaaca tcatttgaaa      540

aaggtattgg tgattggcaa ccacgtggtt cagatgttaa aatttcaatt tcaccaaaag      600

ttgctcattc aggtaaaaaa tcattatttg tttcaaatcg tcaaaaaggt tggcatggtg      660

ctcaaatttc attaaaaggt attttaaaaa caggtaaaac atatgctttt gaagcttggg      720

tttatcaaga atcaggtcaa gatcaaacaa ttattatgac aatgcaacgt aaatattcat      780

cagattcatc aacaaaatat gaatggatta aagctgctac agttccatca ggtcaatggg      840

ttcaattatc aggtacatat acaattccag ctggtgttac agttgaagat ttaacattat      900

attttgaatc acaaaatcca acattagaat tttatgttga tgatgttaaa gttgttgata      960

caacatcagc tgaaattaaa ttagaaatga atccagaaga agaaattcca gctttaaaag     1020

atgttttaaa agattatttt cgtgttggtg ttgctttacc atcaaaagtt tttattaatc     1080

aaaaagatat tgctttaatt tcaaaacatt ttaattcaat tacagctgaa aatgaaatga     1140

aaccagattc attattagct ggtattgaaa atggtaaatt aaaatttcgt tttgaaacag     1200

ctgataaata tattgaattt gctcaacaaa atggtatggt tgttcgtggt catacattag     1260

tttggcataa tcaaacacca gaatggtttt ttaaagatga aaatggtaat ttattatcaa     1320

aagaagaaat gacagaacgt ttacgtgaat atattcatac agttgttggt cattttaaag     1380

gtaaagttta tgcttgggat gttgttaatg aagctgttga tccaaatcaa ccagatggtt     1440

tacgtcgttc aacatggtat caaattatgg gtccagatta tattgaatta gcttttaaat     1500

ttgctcgtga agctgatcca aatgctaaat tattttataa tgattataat acatttgaac     1560

caaaaaaacg tgatattatt tataatttag ttaaatcatt aaaagaaaaa ggtttaattg     1620

atggtattgg tatgcaatgt catatttcat tagctacaga tattcgtcaa attgaagaag     1680

ctattaaaaa attttcaaca attccaggta ttgaaattca tattacagaa ttagatattt     1740

cagtttatcg tgattcaaca tcaaattatt cagaagctcc acgtacagct ttaattgaac     1800

aagctcataa aatggctcaa ttatttaaaa tttttaaaaa atattcaaat gttattacaa     1860

atgttacatt ttggggttta aaagatgatt attcatggcg tgctacacgt cgtaatgatt     1920

ggccattaat ttttgataaa gattatcaag ctaaattagc ttattgggct attgttgctc     1980

cagaagtttt accaccatta ccaaaagaat caaaaatttc agaaggtgaa gctgttgttg     2040

ttggtatgat ggatgattca tatatgatgt caaaaccaat tgaaatttat gatgaagaag     2100

gtaatgttaa agctacaatt cgtgctattt ggaaagattc aacaatttat gtttatggtg     2160

aagttcaaga tgctacaaaa aaaccagctg aagatggtgt tgctattttt attaatccaa     2220

ataatgaacg tacaccatat ttacaaccag atgatacata tgttgtttta tggacaaatt     2280

ggaaatcaga agttaatcgt gaagatgttg aagttaaaaa atttgttggt ccaggttttc     2340

gtcgttattc atttgaaatg tcaattacaa ttccaggtgt tgaatttaaa aaagattcat     2400

atattggttt tgatgttgct gttattgatg atggtaaatg gtattcatgg tcagatacaa     2460

caaattcaca aaaaacaaat acaatgaatt atggtacatt aaaattagaa ggtgttatgg     2520

ttgctacagc taaatatggt acaccagtta ttgatggtga aattgatgat atttggaata     2580

caacagaaga aattgaaaca aaatcagttg ctatgggttc attagaaaaa aatgctacag     2640

ctaaagttcg tgttttatgg gatgaagaaa atttatatgt tttagctatt gttaaagatc     2700

cagttttaaa taaagataat tcaaatcctt gggaacaaga ttcagttgaa atttttattg     2760

atgaaaataa tcataaaaca ggttattatg aagatgatga tgctcaattt cgtgttaatt     2820

atatgaatga acaatcattt ggtacaggtg cttcagctgc tcgttttaaa acagctgtta     2880

aattaattga aggtggttat attgttgaag ctgctattaa atggaaaaca attaaaccat     2940

caccaaatac agttattggt tttaatgttc aagttaatga tgctaatgaa aaaggtcaac     3000

gtgttggtat tatttcatgg tcagatccaa caaataattc atggcgtgat ccatcaaaat     3060

ttggtaattt acgtttaatt aaatatccat atgatgttcc agattatgct taagcatgc      3119


<210>  9
<211>  8270
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Expression vector pCLE

<400>  9
ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga       60

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg      120

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat      180

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg      240

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata      300

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt      360

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat      420

ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgctgcagcc      660

aacaagtttt aaaacctctt caacgctata ttccacaaaa cggtggattt acatggccag      720

gtgattattt acgtttagaa attgttgaaa tgccaaaatt aaaatcaatc aatattaaaa      780

aaacaagttt aaaacaaaaa attaatgttc aaccagttgg tattatgcca cgtaaatatt      840

taattgaaaa acataatatt aaagtattga aaaagaaact ttctcaagct tattcaacac      900

aacaattaac taaagtggta caagaatata aaaatttaat tcaaaattct ccaccagcta      960

tttaaaaata tgccatattt ttactaattt tatattccaa gacattgtat gcttataaat     1020

aaagaagatg ctttgcatct ctaaagatga gtacaatgtt ttggaatatt tatataatat     1080

attaatatat atagttaaat gaaaaaacta aaaaataagc gttagtgaat aatacttttt     1140

atatataaat tctactatat gtttgagctt ccttttatat aaattttaaa tttataacaa     1200

gttacactct cagtatttcc tgcctttttg aaagtaaagc cacaatgttt acatactagg     1260

cagtggcgat accactgcca ctggcgtcct ccttcggagt atgtaaacat gctaagttta     1320

cttgcccgaa ggggaaggag gaggttctta tttcaaatta cctaagataa agtttccttc     1380

ggggtttata cgtaagaatt taagtaaaat aagttaaaat atgaatgcct tggggcatat     1440

taattccact tactatgagt gattctattg aaacaaaaaa catgggacgt attgtacaaa     1500

ttattggtcc tgttttagat atcgtatttg ctaaaggcca agtaccaaat atttacaacg     1560

ctctaactat tcgtgctaaa aactcagcag gtacagaaat ggctgttact tgtgaagttc     1620

aacaactttt aggtgataac tgtgtacgtg cagtttctat gaacccaaca gaaggtttaa     1680

tgcgtggtat ggaagttgta gatactggta aaccgttaag tgttcctgta ggtaaagtaa     1740

ctttaggacg tattttcaac gttctaggtg aacctgtaga taacatgggt aatgttaaag     1800

ttgaagaaac tttaccaatt caccgtacag ctcctgcttt cgttgattta gatactcgtt     1860

tatcaatttt tgaaacaggt attaaagttg tagacctttt agctccatac cgtcgtggtg     1920

gtaaaattgg tcttttcggt ggtgctggtg taggcaaaac agttttaatt atggaactga     1980

tcaacaacat tgctaaagca cacggtggtg tttctgtatt tgctggtgtt ggtgaacgta     2040

cacgtgaagg taacgacctt tacacagaaa tgaaagaatc tggtgttatt gttgaaaaga     2100

acttatctga ttcaaaagta gctcttgtat acggtcaaat gaacgaacca ccaggtgctc     2160

gtatgcgtgt tgctttaaca gctttaacaa tggctgaata tttccgtgat gttaacaaac     2220

aagacgtatt attcttcatt gataacattt tccgtttcgt acaagctggt gctgaagtat     2280

ctgctttatt aggtcgtatg ccatcagctg taggttacca acctacttta gctacagaaa     2340

tgggtggtct tcaagaacgt attacttcaa ctaaagatgg ttctatcaca tcgattcagg     2400

ctgtatatgt acctgcagat gaccttactg accctgctcc tgctacaaca ttcgctcact     2460

tagatgctac tacagtactt tctcgtaact tagctgctaa aggtatttac cctgcggttg     2520

acccattaga atcaacttca actatgttac aaccgtggat tcttggtgaa aaacactatg     2580

attctgcaca aagcgtaaaa aaaactttac aacgttacaa agaattacaa gatattattg     2640

ctattctagg tttagatgaa ttatctgaag aagacagact tattgtagct cgtgctcgta     2700

aaattgaacg tttcttatca caaccattct tcgtagctga agtatttaca ggttcacctg     2760

gtaaatatgt ttctttagct gaaactattg aaggttttgg taaaattttc gctggtgaat     2820

tagatgattt accagaacaa gcattctact tagtaggtaa cattacagaa gctattagta     2880

aagctgcttc attaaaattt ttatttttca tgatgtttat gtgaataatt tggaaccttc     2940

acgcagatgc tcatgacttt gacagtcata caagtgatct agaagaaatt tctagaaaag     3000

tattcagtgc acactttggt caattaggta tcattttcat ttggttaagt gggtgcgaca     3060

cgaagacgta tatattttta tagtttaaaa agatactttt acactgtagt tgaaaagtat     3120

aagcactttt aaaaatcaaa gcagtataag gcaattattt gcattttgct ttagttcttt     3180

ttattttttc agaaaaactt actttttagt ttcaattatg tctaaaagga tcccgggtac     3240

cgagctccac cgcggtggcg gccgctctag ctagaactag tggatcgcac tctaccgatt     3300

gagttacatc cgctttagta tgttactatt tcttttatta taacttataa aatataatac     3360

ataaagataa attctataat aaaaagctaa gattttattt ttctggcaca tcgtaattta     3420

taaagacagg caaatttaaa caaaagataa ctttagaact taattttaaa aatgtaaaat     3480

gatgtttagg tatttaacct aaacaccata aaaaataaaa acgatgttta tgctattcac     3540

ataaacatca tgaaaaataa aaattaaagt ttgtcaatag tatcaaattc gaatttaatt     3600

tctttccaaa cttcacatgc agcagcaagt tctggagacc atttacaagc tgaacgaatt     3660

acgtcgccac cttcacgagc aaggtcacga ccttcgttac gagcttgagt acaagcttgc     3720

atgcctgcag ttatttgcca actaccttag tgatctcgcc tttcacgtag tgaacaaatt     3780

cttccaactg atctgcgcgc gaggccaagc gatcttcttg tccaagataa gcctgcctag     3840

cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga     3900

catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca     3960

ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat     4020

ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac     4080

ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga     4140

tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca     4200

gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt     4260

ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga     4320

tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat     4380

cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg     4440

tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg     4500

cgataaccgc ttcacgagcc atggatttct ccttataata acaattattt aatttaataa     4560

atacattatt tacaccatta gcaacgcttt taattattat gtgttcataa aaaatgcatg     4620

gttattaaat tagacaaata gtttttttta catcatatgt tatattatac cataaatata     4680

ttttttattg gtttacaaat tatttttact tattttaaag atattgtttt attttttacc     4740

atatgtgtat tcgtaaaagt ataacattaa acagtattta cttaaaaatg ttgaattaag     4800

aaagcttatc gataagcttg atctagagtc gataatacga atagtaatac ggttttataa     4860

taataataat aataataata ataataataa taataataat aaaattctta aaaatataca     4920

aaaaattaac atacaaattt aatcccctcc gggattttaa atactccgta aggaggacgt     4980

cctaactacg gattgaaatc cctaagttta ctctttctcg gggaagggga aggggacgtc     5040

ctaaaataca tttcttcaac tgccctttct ttcctcaccc cttcggggac ggctcataaa     5100

gacaagcttt cctctggtcc cctttgggga aagagacatt tcatttaaaa gacaagcttt     5160

ggttgtcccc tttggggaag caactatttt caggcgaccg acgtatcctc tagctcaaat     5220

ttcttcccct ttggggacga cgctcaatca tacccttcca tcccctacgg ggatatatac     5280

cctttcaggg tattagggta tgggggaagc cgatggacta tttacattcc ttgtcccctt     5340

tgggaatcgg ctttgccgat agatttgttg ggaagtaggg tagggatgta aagcttgtca     5400

aagtttacta aaaaaaagta atatataata tatttaaaaa aagtaatata taatatattt     5460

aaaaaaagta atatataata tatttaaaaa aagtaatata taatatattt aaaaaaagta     5520

atatataata tatttaaaaa aagtaatata taatatattt aaatgtattt aaaaattttt     5580

aagattttta aattatattt ccggacagat tattttagga tcgacaacaa aagttacatt     5640

tatttatatt tatgacaaat aatgatattt atagcgtaca agacagttta gtagaacatt     5700

ttaatggttt gaatataaaa acagaaaacc agtttaaact tttagtaaat aagtactgct     5760

tgtatctaaa aaattcgaca gaaggtctta tttttttgtt taagctatat gtaaacagtt     5820

ctactcgtga gcaatctatt attcacctta gtatcttgaa ttggttaaat actaaatctt     5880

taccccaaag aaaagttact ctaattacct ttttacaaga tttagattta gaatttggta     5940

ctgtaaatta tctacagcaa actgaagtat tggttgtttt aaaatttatc tatagtttac     6000

taattaagaa aaaccaagat cgagcatcgc gagatactcc atcagaggat cctctagagg     6060

cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg     6120

gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc     6180

cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc     6240

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     6300

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     6360

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     6420

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     6480

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     6540

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     6600

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     6660

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     6720

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     6780

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     6840

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     6900

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     6960

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     7020

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     7080

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     7140

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     7200

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     7260

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     7320

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     7380

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     7440

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     7500

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     7560

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     7620

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     7680

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     7740

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     7800

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     7860

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     7920

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     7980

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     8040

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     8100

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     8160

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     8220

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac                8270


<210>  10
<211>  13840
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Expression vector pLM20

<400>  10
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaacgaaga aaaacgaagt      420

tttaacagtt aaaactgcgt taaatatgtt tttaagaaat tatacgagct aaccttcact      480

aagcttcagg aacatttggg gagttttgct gctttgcagc cccgaagggg acgacatttg      540

atgagtttca atgacgttcg atgacgttcg atgacacttt acaatatgtc acgaaggttg      600

accaaaaggt atgaccgaag gtaatagcaa cattgttgtg ttttaaggtc tataaataaa      660

ggggacttgt ttttttttta caactttgtt ataataaaat aaatattatt ttaaagtttg      720

aataatcaag tttgcttaat aaaaatataa aatttttccc cttacgggtg taaataacat      780

taaatccctt tatgactaca aaaaaaacaa tacaatttta tgctaatata gctcaggttc      840

gtaatcacga actaatttct cttagtaaca tccaaacacc ttctcaaggt tcaattacaa      900

ttttttgtaa aacttgtaaa accagcttta caacaacagc acgttcttat cagaacgctc      960

gtaaaacagg ttgtcctcaa tgtaaagcaa aaacaacaag tgaaaactgg aaaggtaaaa     1020

ttcgtacaaa atctcctgaa gaggcctcta aacaggccgt tttaaatgaa tataaacaac     1080

aaaaacactt acaaaaaggt ttagcatatg cacatatttc taataaagaa gatttaaaaa     1140

tttttctgaa agaaagtcca aacgtttata acaattttat actccaacgg attgaccatc     1200

cagttatagg aaaatatact gaaaaccatc atattattcc taaacacacc ggtggtccac     1260

ataaacgctg gaatttaata aaattaacac cagaagacca tatggaagct caccgattac     1320

gtgctttagt atataacgaa gctggtgatc accaagcaat tcgtttcaga actaatcctt     1380

ctgagctagt tgaacgacgt cttcgaggaa accaaatagc caacgaaacg cgtttaagag     1440

aacgtactgg tatctatgcc gaaggcgctt cttctaaagg tggacgtatt ggtggtctag     1500

taaaaagtca cgaaaaagat ttaaaacaaa gtacaaaaat gagccaaccc gtattaaaag     1560

ctctttacga aggttcacgt tggaaacatt tacaatccgg aactgaacta aaccttcaac     1620

caaacagatt gtttacgctt ccgcagctag ttcaaaaact tttagaagct ttaccaccat     1680

gtaaagataa agaagtttta gaaagagcaa aaacaagtac agttacttca aatctggctc     1740

gtgtaattaa aaaacaacga ccttctgcat atggctggat aacgttttaa acctttttac     1800

gttttttgta ataatatggc aacctgcagc gaagctaggg atgggcattg cttccctaga     1860

acgttcagag actagagctt gagtcccaac aataatagct cactagcgcc cggcatctaa     1920

aatggtttaa attttagatg gtgatatagt ccaacccacc ttgaaaaagg tggacgacga     1980

ccagtagatt aaaaaggttt ttagtctata ttaaatggtt actttggtcg tctaatcttc     2040

caatacgctt ctttcaacaa ctctcgttca ttacacttct tcttagctgc ttggccggta     2100

atcggtattt ggttcactgc tttaggttta tcaactatgg cattcaactt aaacggtttc     2160

aacttcaacc aatcagtagt agactcacaa ggtcgtgtac taaacacttg ggcagacatc     2220

atcaaccgtg ctaacttagg tatggaagta atgcacgagc gtaacgctca caacttccct     2280

ctagacttag cttcaactaa ctctagctca aacaactaat ttttttttaa actaaaataa     2340

atctggttaa ccatacctgg tttattttag tttatacaca cttttcatat atatatactt     2400

aatagctacc ataggcagtt ggcaggacgt ccccttacgg gacaaatgta tttattgttg     2460

cctgccaact gcctaatata aatattagtg gacgtcccct tccccttacg ggcaagtaaa     2520

cttagggatt ttaatgctcc gttaggaggc aaataaattt tagtggcagt tgcctcgcct     2580

atcggctaac aagttccttc ggagtatata aatatcctgc caactgccga tatttatata     2640

ctaggcagtg gcggtaccac tcgactaata tttatattcc gtaagacgtc ctccttcgga     2700

gtatgtaaac atgctaagtt tacttgccca atatttatat taggacgtca gtggcagtgg     2760

accaccactc gtattttata ctcgaaaggc agttggcagg caactcgact aaaatttatt     2820

tacccgaaga cgtcccgaag aaggggaagg aggcagtggt accaccactg gctccgcagt     2880

attaacatcc tatatttata tactccgaag aacttcttag ccgatggcaa ctgccacaaa     2940

aaaccatcgg cttcgtttca cacagatggt gagaaccaca cgtttcgtcc tataaaaata     3000

gctaagttta cttgcccaat atttatatta ggacgtcccc ttcggtaaat aaattttagt     3060

ggctgtgtga cgttcactgg cgtcttggta ggttctgtga ctgactaaat aaaaaagtat     3120

ttgtcgtcta cgatatgtaa atctgtcgta tacgatatgt aaatttgagc tcttatggcc     3180

tctacatcga ggtttattat cttaccgaag gtaaatgcct tcgtggatct tatgggacgt     3240

cctgtgtcct tcctagtggt caataatcac ttcgtgacag cctgggctta catttatata     3300

agcgctgtta tatttatacg ctgttagaca aggtttaaat acataaattt ttattagtct     3360

atcgaccgtt aattgcttaa cttaccgaag gttaatcgct ttacttaccg aaggttaatc     3420

gctttactta ccgaaggtta attgcttaac ttactgaagg ttaatcgctt tacttaccga     3480

aggttaattg cttaacttac tgaaggttaa tcgctttact taccgaaggt taatccaatt     3540

tttttttcgc catatgtaga cgtttaattg ctacaacgtt attagccttt cgtcgctatc     3600

aaatcggttc agatatatat cactttattc actttcgttt atattatggc tggattaggt     3660

cttttagtta attaaaattt acatatttaa tgctatttat tattattgca attgcattaa     3720

atattttttt aaaaaaaatt aatcttcagc tatattagta aataacccat aaatagtttc     3780

aattggaata attggaattg gatatggact agttttattt tcttctaata actttaatat     3840

cgctggatct taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccc     3900

gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt     3960

ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat     4020

tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc     4080

gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt     4140

cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa     4200

ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc     4260

cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat     4320

ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct     4380

catgggccgc tctagctaga actagtggat cgcactctac cgattgagtt acatccgctt     4440

tagtatgtta ctatttcttt tattacttat aaaatataat acataaagat aaattctata     4500

ataaaaagct aagattttat ttttctggca catcgtaatt tataaagcac aggcaaattt     4560

aaacaaaaga taactttaga acttaatttt aaaaatgtaa aatgatgttt aggtatttaa     4620

cctaaacacc ataaaaaata aaaacgatgt ttatgctatt cacataaaca tcatgaaaaa     4680

taaaaattaa agtttgtcaa tagtatcaaa ttcgaattta atttctttcc aaacttcaca     4740

tgcagcagca agttctggag accatttaca agctgaacga attacgtcgc caccttcacg     4800

agcaaggtca cgaccttcgt tacgagcttg agtacaagct tgcatgcctg cagttatttg     4860

ccaactacct tagtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg     4920

cgcgaggcca agcgatcttc ttgtccaaga taagcctgcc tagcttcaag tatgacgggc     4980

tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt     5040

ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg     5100

ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga     5160

tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta     5220

tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag     5280

atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga     5340

taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc     5400

tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt     5460

gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg     5520

ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc     5580

tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgataac cgcttcacga     5640

gccatggaca ttttcacttc tggagtgtat tgttcaatta aatctttaat aagattacta     5700

agttcttctg gagtacgcat tgccataaaa aagaaaaaat aaataaaaga ttaaaaaagt     5760

ttatttttaa aatctttctc gagaatttta aataagttta aaattcaaca aaaatagtga     5820

gtggtaagat cacttgttaa caaaagtaat ggttcaccct tgtcatattt aaatactaaa     5880

attcatttgc ccgaagagga caaatttatt tattgcatta aaatccctaa gtttacttgc     5940

ccgtaagggg aaggggggga cgtccacagg cgtcgtaagc aactaaagtt tatgacgccg     6000

attgctttgt taggaaaata taaatatccc ataagaaaag gtcctttaaa ggttttatgg     6060

actaaataaa aaagatagca taagcattaa aatcatgcaa attaaaaaaa aggtaaatgt     6120

atttataaaa aggtaaatgt atttatatag tatttatatt atagcataat aataaatata     6180

tttataaatt gattgttctt agagctaaaa gagaagaaca atgggtttat aggtattttg     6240

agaccagtta taaaaatgac ttttgacgtt taggtatata aacactgcct ctaataaagt     6300

catcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct     6360

gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca     6420

agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct     6480

cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga     6540

cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt     6600

tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc     6660

agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg     6720

agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag     6780

cgctcatgcg ataccgtcga cctcgagggg gggcccggta cccagctttt gttcccttta     6840

gtgagggtta atcccgggta ccgagctcca ccgcggtggc ggccgctcta gctagaacta     6900

gtggatcgca ctctaccgat tgagttacat ccgctttagt atgttactat ttcttttatt     6960

acttataaaa tataatacat aaagataaat tctataataa aaagctaaga ttttattttt     7020

ctggcacatc gtaatttata aagcacaggc aaatttaaac aaaagataac tttagaactt     7080

aattttaaaa atgtaaaatg atgtttaggt atttaaccta aacaccataa aaaataaaaa     7140

cgatgtttat gctattcaca taaacatcat gaaaaataaa aattaaagtt tgtcaatagt     7200

atcaaattcg aatttaattt ctttccaaac ttcacatgca gcagcaagtt ctggagacca     7260

tttacaagct gaacgaatta cgtcgccacc ttcacgagca aggtcacgac cttcgttacg     7320

agcttgagta caagcttgca tgcttataat gtgaaatcaa ctaatggagc tgtaccacgt     7380

gtaccatttt gaatatcaac accttcagca tttgattttt taacagcaac ttcaccttga     7440

tttgtttcaa cttcgaataa aaaacgagct tgacgaccag ctgtaataga agcaccaaca     7500

tcagcagcta catcagctgg ttttaatgta acagcaccac ctgatggtaa tgtaactgat     7560

aatgctttac gtgtaactaa aactgtacct tcaccacgac cattagcagc atgatcagca     7620

tattctgtaa ttgtaacgaa aattttacca ccttctgttg gtaaaatgtt tgaaacatca     7680

cgaatgtgaa ttaaatttgc tgatgttgta cctgttgtaa ttgtatcgaa tttcatacca     7740

tcacgtgtta aaatgaataa atcagctaat tcatctttga aataattata tgttgcttgt     7800

gaatcacctt gaatttcagc tttaacattg aagtttacag ctggaactgg aactgtattg     7860

tcagcagcaa catataatgt accgaaagca gctaaagcat tttgaacacc acctgccata     7920

ttaccagcac ctaaaacagc tgtagctgat tgaccatcag cagcagcttt ccaaccaatt     7980

gaagcaccag ctttattaac taaattacca ttagcatctt ttttaaaagc tgaaacatca     8040

cctgttaaag ttaaatttac tttatcacgt tgaattgttt gatttgttgt aacatttgta     8100

atagcgaaat ctgtagctga aatattagca tcaccaatat ttaattttaa aaaaccttct     8160

gaaactaaag ctgtagcttt accagcattt gttaatgata attggaataa tttacctaat     8220

ttgaataatg gttttgtagc aactggatca gctgtatatg atgcattcca taaagcacca     8280

gctgttaaag caactgtacc ttgtgaacct gatgttaatt taaatgcttg attagctaag     8340

tttaatttta attcaacttc agcatcagcg aaattaattg tttcttcaac atctgaagct     8400

aaagctaaac gtaaacgttt accatttgaa tcaattgtgt gaacaattgt taattgtgta     8460

ccatctgaaa taattttgaa gaatttacga acatcattaa cttcacctga attagcaaaa     8520

ttaccgtgat catttggatt aactaataaa cctgtaaatg gacgacgcca aaaaccattt     8580

gataaccatt gacgaatttc agcatcattg aattgacctt caccttgaat ttctaaaaca     8640

atgaaaccat tattttttaa atcaccaact tcagagaaat ttaatgttaa attttgttga     8700

gctgttgaaa aatctaaaac tggttgtttt gtaactgaag ctaatgatgt tgtaacgaat     8760

gtattatcat ttggtgaaat aacaacatca gcaaaagctg gagcagctga accaacaaca     8820

atagcagcag caattaatgt ttttttgaac atagccatgg acattttcac ttctggagtg     8880

tattgttcaa ttaaatcttt aataagatta ctaagttctt ctggagtacg cattgccata     8940

aaaaagaaaa aataaataaa agattaaaaa agtttatttt taaaatcttt ctcgagaatt     9000

ttaaataagt ttaaaattca acaaaaatag tgagtggtaa gatcacttgt taacaaaagt     9060

aatggttcac ccttgtcata tttaaatact aaaattcatt tgcccgaaga ggacaaattt     9120

atttattgca ttaaaatccc taagtttact tgcccgtaag gggaaggggg ggacgtccac     9180

aggcgtcgta agcaactaaa gtttatgacg ccgattgctt tgttaggaaa atataaatat     9240

cccataagaa aaggtccttt aaaggtttta tggactaaat aaaaaagata gcataagcat     9300

taaaatcatg caaattaaaa aaaaggtaaa tgtatttata aaaaggtaaa tgtatttata     9360

tagtatttat attatagcat aataataaat atatttataa attgattgtt cttagagcta     9420

aaagagaaga acaatgggtt tataggtatt ttgagaccag ttataaaaat gacttttgac     9480

gtttaggtat ataaacactg cctctaataa agtcatcgat atcggatccg tatccatgct     9540

agcaatatct gatggtactt gcatttcata agtttggcct ggaataacca ccgtttcgga     9600

agtacctgtc gctttaagtt ttatagctaa atctaaagtt tctttaagtc ttttagctgt     9660

attaaatact ccacgacttt cccttacggg acaataaata aatttgtccc cttcccctta     9720

cgtgacgtca gtggcagttg cctgccaact gcctccttcg gagtattaaa atcctatatt     9780

tatatactcc taagtttact tgcccaatat ttatattagg cagttggcag gcaactgcca     9840

ctgacgtccc gaaggggaag gggaaggacg tccccttcgg gtaaataaat tttagtggca     9900

gtggtaccac cactgcctgc ttcctccttc cccttcgggc aagtaaactt agaataaaat     9960

ttatttgctg cgctagcagg tttacatact cctaagttta cttgcccgaa ggggaaggag    10020

gacgtcccct tacgggaata taaatattag tggcagtggt acaataaata aattgtatgt    10080

aaaccccttc gggcaactaa agtttatcgc agtatataaa tatagaatgt ttacatactc    10140

cgaaggagga cgccagtggc agtggtaccg ccactgcctg tccgcagtat taacatccta    10200

ttttaatact ccgaaggagg cagttggcag gcaactgcca ctaatattta tattcccgta    10260

aggggacgtc ctaatttaat actccgaagg aggcagttgg caggcaactg ccactaaaat    10320

ttatttgcct cctaacggag cattaaaatc ccgaagggga cgtcccgaag gggaagggga    10380

aggaggcaac tgcctgcttc ctccttcccc ttcgggcaag taaacttaga ataaaattta    10440

tttgctgcgc tagcaggttt acatactcct aagtttactt gcccgaaggg gaaggaggac    10500

gtccccttac gggaatataa atattagtgg cagtggtaca ataaataaat tgtatgtaaa    10560

ccccttcggg caactaaagt ttatcgcagt atataaatat cggcagttgg caggcaactg    10620

ccactaaaat tcatttgccc gaaggggacg tccactaata tttatattcc cgtaagggga    10680

cgtcccgaag gggaagggga cgtcctaaac ggagcattaa aatccctaag tttacttgcc    10740

taggcagttg gcaggatatt tatatacgat attaatactt ttgctactgg cacactaaaa    10800

tttatttgcc cgtaagggga cgtccttcgg tggttatata aataatcccg tagggggagg    10860

gggatgtccc gtagggggag gggagtggag gctccaacgg aggttggagc ttctttggtt    10920

tcctaggcat tatttaaata ttttttaacc ctagcactag aactgagatt ccagacggcg    10980

acccgtaaag ttcttcagtc ccctcagctt tttcacaacc aagttcggga tggattggtg    11040

tgggtccaac tgagcaaaga gcaccaaggt taactgcatc tctgtgagat gctagttaaa    11100

ctaagcttag cttagctcat aaacgatagt tacccgcaag gggttatgta attatattat    11160

aaggtcaaaa tcaaacggcc tttagtatat ctcggctaaa gccattgctg actgtacacc    11220

tgatacctat ataacggctt gtctagccgc ggccttagag agcactcatc ttgagtttag    11280

cttcctactt agatgctttc agcagttatc tatccatgcg tagctaccca gcgtttccca    11340

ttggaatgag aactggtaca caattggcat gtcctttcag gtcctctcgt actatgaaag    11400

gctactctca atgctctaac gcctacaccg gatatggacc aaactgtctc acgcatgaaa    11460

ttttaaagcc gaataaaact tgcggtcttt aaaactaacc cctttacttt cgtaaaggca    11520

tggactatgt cttcatcctg ctactgttaa tggcaggagt cggcgtatta tactttccca    11580

ctctcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga    11640

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc    11700

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc    11760

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc    11820

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt    11880

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca    11940

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa    12000

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat    12060

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc    12120

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc    12180

gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt    12240

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac    12300

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg    12360

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca    12420

gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc    12480

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa    12540

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa    12600

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac    12660

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta    12720

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt    12780

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata    12840

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc    12900

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac    12960

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag    13020

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac    13080

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc    13140

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg    13200

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc    13260

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct    13320

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc    13380

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc    13440

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc    13500

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc    13560

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca    13620

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt    13680

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt    13740

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca    13800

ttaacctata aaaataggcg tatcacgagg ccctttcgtc                          13840


<210>  11
<211>  338
<212>  PRT
<213>  Pseudomonas stutzeri

<400>  11

Met Leu Pro Lys Leu Val Ile Thr His Arg Val His Asp Glu Ile Leu 
1               5                   10                  15      


Gln Leu Leu Ala Pro His Cys Glu Leu Met Thr Asn Gln Thr Asp Ser 
            20                  25                  30          


Thr Leu Thr Arg Glu Glu Ile Leu Arg Arg Cys Arg Asp Ala Gln Ala 
        35                  40                  45              


Met Met Ala Phe Met Pro Asp Arg Val Asp Ala Asp Phe Leu Gln Ala 
    50                  55                  60                  


Cys Pro Glu Leu Arg Val Val Gly Cys Ala Leu Lys Gly Phe Asp Asn 
65                  70                  75                  80  


Phe Asp Val Asp Ala Cys Thr Ala Arg Gly Val Trp Leu Thr Phe Val 
                85                  90                  95      


Pro Asp Leu Leu Thr Val Pro Thr Ala Glu Leu Ala Ile Gly Leu Ala 
            100                 105                 110         


Val Gly Leu Gly Arg His Leu Arg Ala Ala Asp Ala Phe Val Arg Ser 
        115                 120                 125             


Gly Glu Phe Gln Gly Trp Gln Pro Gln Phe Tyr Gly Thr Gly Leu Asp 
    130                 135                 140                 


Asn Ala Thr Val Gly Ile Leu Gly Met Gly Ala Ile Gly Leu Ala Met 
145                 150                 155                 160 


Ala Asp Arg Leu Gln Gly Trp Gly Ala Thr Leu Gln Tyr His Glu Ala 
                165                 170                 175     


Lys Ala Leu Asp Thr Gln Thr Glu Gln Arg Leu Gly Leu Arg Gln Val 
            180                 185                 190         


Ala Cys Ser Glu Leu Phe Ala Ser Ser Asp Phe Ile Leu Leu Ala Leu 
        195                 200                 205             


Pro Leu Asn Ala Asp Thr Gln His Leu Val Asn Ala Glu Leu Leu Ala 
    210                 215                 220                 


Leu Val Arg Pro Gly Ala Leu Leu Val Asn Pro Cys Arg Gly Ser Val 
225                 230                 235                 240 


Val Asp Glu Ala Ala Val Leu Ala Ala Leu Glu Arg Gly Gln Leu Gly 
                245                 250                 255     


Gly Tyr Ala Ala Asp Val Phe Glu Met Glu Asp Trp Ala Arg Ala Asp 
            260                 265                 270         


Arg Pro Arg Leu Ile Asp Pro Ala Leu Leu Ala His Pro Asn Thr Leu 
        275                 280                 285             


Phe Thr Pro His Ile Gly Ser Ala Val Arg Ala Val Arg Leu Glu Ile 
    290                 295                 300                 


Glu Arg Cys Ala Ala Gln Asn Ile Ile Gln Val Leu Ala Gly Ala Arg 
305                 310                 315                 320 


Pro Ile Asn Ala Ala Asn Arg Leu Pro Lys Ala Glu Pro Ala Ala Cys 
                325                 330                 335     


Glu Phe 
        


<210>  12
<211>  1017
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon optimized sequence of PTXD from Pseudomonas stutzeri

<400>  12
atgctgccga agctggtcat cacccaccgc gtccacgacg agatcctgca gctgctggcc       60

ccgcactgcg agctgatgac gaaccagacc gactcgaccc tgacgcgcga ggagatcctg      120

cgccgctgcc gcgacgcgca ggctatgatg gccttcatgc cggaccgcgt ggacgctgac      180

ttcctgcagg cttgcccgga gctgcgcgtg gtcggctgcg ctctgaaggg cttcgacaac      240

ttcgacgtgg acgcttgcac cgctcgcggc gtgtggctga cgttcgtccc ggacctgctg      300

accgtgccga cggctgagct ggccatcggc ctggctgtcg gcctgggccg ccacctgcgc      360

gccgcggacg ctttcgtgcg ctccggcgag ttccagggct ggcagccgca gttctacggc      420

accggcctgg acaacgctac ggtcggcatc ctgggcatgg gcgctatcgg cctggctatg      480

gctgaccgcc tgcagggctg gggcgctacc ctgcagtacc acgaggctaa ggccctggac      540

acccagacgg agcagcgcct gggcctgcgc caggtggctt gcagcgagct gttcgcctcg      600

tccgacttca tcctgctggc tctgccgctg aacgctgaca cccagcacct ggtcaacgct      660

gagctgctgg ctctggtgcg ccccggcgct ctgctggtca acccgtgccg cggctctgtg      720

gtggacgagg ctgccgtgct ggctgctctg gagcgcggcc agctgggcgg ctacgccgcg      780

gacgtcttcg agatggagga ctgggcgcgc gctgaccgcc cgcgcctgat cgacccggct      840

ctgctggctc acccgaacac cctgttcacg ccgcacatcg gcagcgccgt gcgcgcggtc      900

cgcctggaga tcgagcgctg cgctgcccag aacatcatcc aggtgctggc cggcgcccgc      960

ccgatcaacg ctgccaaccg cctgccgaag gctgagccgg ctgcttgcga attctaa        1017


<210>  13
<211>  4657
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Construct for the expression of PTXD

<400>  13
tcgctgaggc ttgacatgat tggtgcgtat gtttgtatga agctacagga ctgatttggc       60

gggctatgag ggcgggggaa gctctggaag ggccgcgatg gggcgcgcgg cgtccagaag      120

gcgccatacg gcccgctggc ggcacccatc cggtataaaa gcccgcgacc ccgaacggtg      180

acctccactt tcagcgacaa acgagcactt atacatacgc gactattctg ccgctataca      240

taaccactca gctagcttaa gatcccatca agcttgcatg ccgggcgcgc cagaaggagc      300

gcagccaaac caggatgatg tttgatgggg tatttgagca cttgcaaccc ttatccggaa      360

gccccctggc ccacaaaggc taggcgccaa tgcaagcagt tcgcatgcag cccctggagc      420

ggtgccctcc tgataaaccg gccagggggc ctatgttctt tactttttta caagagaagt      480

cactcaacat cttaaaatgg ccaggtgagt cgacgagcaa gcccggcgga tcaggcagcg      540

tgcttgcaga tttgacttgc aacgcccgca ttgtgtcgac gaaggctttt ggctcctctg      600

tcgctgtctc aagcagcatc taaccctgcg tcgccgtttc catttgcagg atggccatgc      660

atatggccaa gctgaccagc gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg      720

tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg      780

gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtgagtcgac      840

gagcaagccc ggcggatcag gcagcgtgct tgcagatttg acttgcaacg cccgcattgt      900

gtcgacgaag gcttttggct cctctgtcgc tgtctcaagc agcatctaac cctgcgtcgc      960

cgtttccatt tgcaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc     1020

ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc     1080

tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc     1140

gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggacgcccc ggtgaagcag     1200

accctgaact tcgacctgct gaagctggcg ggcgacgtgg agagcaaccc gggccccgaa     1260

ttcatgctgc cgaagctggt catcacccac cgcgtccacg acgagatcct gcagctgctg     1320

gccccgcact gcgagctgat gacgaaccag accgactcga ccctgacgcg cgaggagatc     1380

ctgcgccgct gccgcgacgc gcaggctatg atggccttca tgccggaccg cgtggacgct     1440

gacttcctgc aggcttgccc ggagctgcgc gtggtcggct gcgctctgaa gggcttcgac     1500

aacttcgacg tggacgcttg caccgctcgc ggcgtgtggc tgacgttcgt cccggacctg     1560

ctgaccgtgc cgacggctga gctggccatc ggcctggctg tcggcctggg ccgccacctg     1620

cgcgccgcgg acgctttcgt gcgctccggc gagttccagg gctggcagcc gcagttctac     1680

ggcaccggcc tggacaacgc tacggtcggc atcctgggca tgggcgctat cggcctggct     1740

atggctgacc gcctgcaggg ctggggcgct accctgcagt accacgaggc taaggccctg     1800

gacacccaga cggagcagcg cctgggcctg cgccaggtgg cttgcagcga gctgttcgcc     1860

tcgtccgact tcatcctgct ggctctgccg ctgaacgctg acacccagca cctggtcaac     1920

gctgagctgc tggctctggt gcgccccggc gctctgctgg tcaacccgtg ccgcggctct     1980

gtggtggacg aggctgccgt gctggctgct ctggagcgcg gccagctggg cggctacgcc     2040

gcggacgtct tcgagatgga ggactgggcg cgcgctgacc gcccgcgcct gatcgacccg     2100

gctctgctgg ctcacccgaa caccctgttc acgccgcaca tcggcagcgc cgtgcgcgcg     2160

gtccgcctgg agatcgagcg ctgcgctgcc cagaacatca tccaggtgct ggccggcgcc     2220

cgcccgatca acgctgccaa ccgcctgccg aaggctgagc cggctgcttg cgaattctaa     2280

ctcgagcacc accaccacca ccacggcaag cccatcccca accccctgct gggcctggac     2340

agcaccgaga acctgtactt ccagggggta ccaggatccg gaagatctgg tctagagggc     2400

aagcccatcc ccaaccccct gctgggcctg gacagcaccc gtaccggtca ccaccaccac     2460

caccactaac tgcagccgct ccgtgtaaat ggaggcgctc gttgatctga gccttgcccc     2520

ctgacgaacg gcggtggatg gaagatactg ctctcaagtg ctgaagcggt agcttagctc     2580

cccgtttcgt gctgatcagt ctttttcaac acgtaaaaag cggaggagtt ttgcaatttt     2640

gttggttgta acgatcctcc gttgattttg gcctctttct ccatgggcgg gctgggcgta     2700

tttgaagcgg cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga     2760

acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagattatca     2820

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt     2880

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca     2940

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg     3000

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca     3060

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt     3120

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt     3180

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca     3240

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca     3300

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga     3360

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact     3420

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga     3480

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg     3540

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc     3600

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga     3660

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat     3720

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt     3780

caatattatt gaagcattta tcagggttat tgtctcatga ccaaaatccc ttaacgtgag     3840

ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct     3900

ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt     3960

tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg     4020

cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct     4080

gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgt tgccagtggc     4140

gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg     4200

tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa     4260

ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg     4320

gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg     4380

ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga     4440

tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt     4500

ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct     4560

gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga     4620

acgaccgagc gcagcgagtc agtgagcgag gaagcgg                              4657


<210>  14
<211>  462
<212>  PRT
<213>  Thermus thermophilus

<400>  14

Met Leu Ala Arg Arg Ser Phe Leu Gln Ala Ala Ala Gly Ser Leu Val 
1               5                   10                  15      


Leu Gly Leu Ala Arg Ala Gln Gly Pro Ser Phe Pro Glu Pro Lys Val 
            20                  25                  30          


Val Arg Ser Gln Gly Gly Leu Leu Ser Leu Lys Leu Ser Ala Thr Pro 
        35                  40                  45              


Thr Pro Leu Ala Leu Ala Gly Gln Arg Ala Thr Leu Leu Thr Tyr Gly 
    50                  55                  60                  


Gly Ser Phe Pro Gly Pro Thr Leu Arg Val Arg Pro Arg Asp Thr Val 
65                  70                  75                  80  


Arg Leu Thr Leu Glu Asn Arg Leu Pro Glu Pro Thr Asn Leu His Trp 
                85                  90                  95      


His Gly Leu Pro Ile Ser Pro Lys Val Asp Asp Pro Phe Leu Glu Ile 
            100                 105                 110         


Pro Pro Gly Glu Ser Trp Thr Tyr Glu Phe Thr Val Pro Lys Glu Leu 
        115                 120                 125             


Ala Gly Thr Phe Trp Tyr His Pro His Leu His Gly Arg Val Ala Pro 
    130                 135                 140                 


Gln Leu Phe Ala Gly Leu Leu Gly Ala Leu Val Val Glu Ser Ser Leu 
145                 150                 155                 160 


Asp Ala Ile Pro Glu Leu Arg Glu Ala Glu Glu His Leu Leu Val Leu 
                165                 170                 175     


Lys Asp Leu Ala Leu Gln Gly Gly Arg Pro Ala Pro His Thr Pro Met 
            180                 185                 190         


Asp Trp Met Asn Gly Lys Glu Gly Asp Leu Val Leu Val Asn Gly Ala 
        195                 200                 205             


Leu Arg Pro Thr Leu Val Ala Gln Lys Ala Thr Leu Arg Leu Arg Leu 
    210                 215                 220                 


Leu Asn Ala Ser Asn Ala Arg Tyr Tyr Arg Leu Ala Leu Gln Asp His 
225                 230                 235                 240 


Pro Leu Tyr Leu Ile Ala Ala Asp Gly Gly Phe Leu Glu Glu Pro Leu 
                245                 250                 255     


Glu Val Ser Glu Leu Leu Leu Ala Pro Gly Glu Arg Ala Glu Val Leu 
            260                 265                 270         


Val Arg Leu Arg Lys Glu Gly Arg Phe Leu Leu Gln Ala Leu Pro Tyr 
        275                 280                 285             


Asp Arg Gly Ala Met Gly Met Met Asp Met Gly Gly Met Ala His Ala 
    290                 295                 300                 


Met Pro Gln Gly Pro Ser Arg Pro Glu Thr Leu Leu Tyr Leu Ile Ala 
305                 310                 315                 320 


Pro Lys Asn Pro Lys Pro Leu Pro Leu Pro Lys Ala Leu Ser Pro Phe 
                325                 330                 335     


Pro Thr Leu Pro Ala Pro Val Val Thr Arg Arg Leu Val Leu Thr Glu 
            340                 345                 350         


Asp Met Met Ala Ala Arg Phe Phe Ile Asn Gly Gln Val Phe Asp His 
        355                 360                 365             


Arg Arg Val Asp Leu Lys Gly Gln Ala Gln Thr Val Glu Val Trp Glu 
    370                 375                 380                 


Val Glu Asn Gln Gly Asp Met Asp His Pro Phe His Leu His Val His 
385                 390                 395                 400 


Pro Phe Gln Val Leu Ser Val Gly Gly Arg Pro Phe Pro Tyr Arg Ala 
                405                 410                 415     


Trp Lys Asp Val Val Asn Leu Lys Ala Gly Glu Val Ala Arg Leu Leu 
            420                 425                 430         


Val Pro Leu Arg Glu Lys Gly Arg Thr Val Phe His Cys His Ile Val 
        435                 440                 445             


Glu His Glu Asp Arg Gly Met Met Gly Val Leu Glu Val Gly 
    450                 455                 460         


<210>  15
<211>  1400
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon usage optimized nucleotidic sequence encoding for laccase 
       of Thermus thermophilus

<400>  15
ccatggcttt agctcgtcgt tcatttttac aagctgctgc tggttcatta gttttaggtt       60

tagctcgtgc tcaaggtcca tcatttccag aaccaaaagt tgttcgttca caaggtggtt      120

tattatcatt aaaattatca gctacaccaa caccattagc tttagctggt caacgtgcta      180

cattattaac atatggtggt tcatttccag gtccaacatt acgtgttcgt ccacgtgata      240

cagttcgttt aacattagaa aatcgtttac cagaaccaac aaatttacat tggcatggtt      300

taccaatttc accaaaagtt gatgatccat ttttagaaat tccaccaggt gaatcatgga      360

catatgaatt tacagttcca aaagaattag ctggtacatt ttggtatcat ccacatttac      420

atggtcgtgt tgctccacaa ttatttgctg gtttattagg tgctttagtt gttgaatcat      480

cattagatgc tattccagaa ttacgtgaag ctgaagaaca tttattagtt ttaaaagatt      540

tagctttaca aggtggtcgt ccagctccac atacaccaat ggattggatg aatggtaaag      600

aaggtgattt agttttagtt aatggtgctt tacgtccaac attagttgct caaaaagcta      660

cattacgttt acgtttatta aatgcttcaa atgctcgtta ttatcgttta gctttacaag      720

atcatccatt atatttaatt gctgctgatg gtggtttttt agaagaacca ttagaagttt      780

cagaattatt attagctcca ggtgaacgtg ctgaagtttt agttcgttta cgtaaagaag      840

gtcgtttttt attacaagca ttaccatatg atcgtggtgc tatgggtatg atggatatgg      900

gtggtatggc tcatgctatg ccacaaggtc catcacgtcc agaaacatta ttatatttaa      960

ttgctccaaa aaatccaaaa ccattaccat taccaaaagc attatcacca tttccaacat     1020

taccagctcc agttgttaca cgtcgtttag ttttaacaga agatatgatg gctgctcgtt     1080

tttttattaa tggtcaagtt tttgatcatc gtcgtgttga tttaaaaggt caagctcaaa     1140

cagttgaagt ttgggaagtt gaaaatcaag gtgatatgga tcatccattt catttacatg     1200

ttcatccatt tcaagtttta tcagttggtg gtcgtccatt tccatatcgt gcttggaaag     1260

atgttgttaa tttaaaagct ggtgaagttg ctcgtttatt agttccatta cgtgaaaaag     1320

gtcgtacagt ttttcattgt catattgttg aacatgaaga tcgtggtatg atgggtgttt     1380

tagaagttgg ttaagcatgc                                                 1400


<210>  16
<211>  242
<212>  PRT
<213>  Thermus thermophilus

<400>  16

Met Thr Leu Leu Arg Thr Pro Leu Pro Val Pro His Gly Phe Thr Thr 
1               5                   10                  15      


Arg Glu Gly Gly Val Ser Glu Gly Pro Phe Arg Ser Leu Asn Leu Ser 
            20                  25                  30          


Ala Ala Thr Gly Asp Asp Pro Glu Arg Val Ala Glu Asn Gln Arg Arg 
        35                  40                  45              


Val Leu Ala Ala Phe Gly His Pro Pro Val Ala Gly Leu Arg Gln Val 
    50                  55                  60                  


His Gly Thr Glu Val His Pro Val Glu Gly Pro Gly Leu Trp Glu Gly 
65                  70                  75                  80  


Asp Gly Leu Leu Thr Arg Thr Pro Gly Leu Leu Leu Arg Val Gly Val 
                85                  90                  95      


Ala Asp Cys Tyr Pro Leu Leu Leu Tyr His Pro Lys Gly Ala Val Gly 
            100                 105                 110         


Ala Leu His Ala Gly Trp Arg Gly Val Val Gly Gly Ile Leu Pro Lys 
        115                 120                 125             


Ala Leu Glu Arg Leu Glu Ala Val Tyr Arg Leu Asp Pro Thr Glu Val 
    130                 135                 140                 


His Leu Ala Ile Gly Pro Gly Ile Gly Gly Ala Cys Tyr Gln Val Gly 
145                 150                 155                 160 


Glu Glu Val Val Ala Arg Phe Ala Glu Ala Gly Leu Phe Thr Phe Arg 
                165                 170                 175     


Glu Asp Pro Ala Ala Pro Gly Lys Tyr Leu Leu Asp Leu Glu Lys Ala 
            180                 185                 190         


Leu Leu Leu Gln Ala Arg Arg Ala Gly Leu Arg Glu Glu Arg Ile Tyr 
        195                 200                 205             


Arg Val Gly Leu Cys Thr His Cys Ala Pro Asn Leu Phe Ser His Arg 
    210                 215                 220                 


Arg Asp Arg Gly Arg Thr Gly Arg Met Trp Gly Leu Val Met Leu Pro 
225                 230                 235                 240 


Pro Arg 
        


<210>  17
<211>  740
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Codon usage optimized nucleotidic sequence of polyphenol oxidase 
       from Thermus thermophilus

<400>  17
ccatggctac attattacgt acaccattac cagttccaca tggttttaca acacgtgaag       60

gtggtgtttc agaaggtcca tttcgttcat taaatttatc agctgctaca ggtgatgatc      120

cagaacgtgt tgctgaaaat caacgtcgtg ttttagctgc ttttggtcat ccaccagttg      180

ctggtttacg tcaagttcat ggtacagaag ttcatccagt tgaaggtcca ggtttatggg      240

aaggtgatgg tttattaaca cgtacaccag gtttattatt acgtgttggt gttgctgatt      300

gttatccatt attattatat catccaaaag gtgctgttgg tgctttacat gctggttggc      360

gtggtgttgt tggtggtatt ttaccaaaag cattagaacg tttagaagct gtttatcgtt      420

tagatccaac agaagttcat ttagctattg gtccaggtat tggtggtgct tgttatcaag      480

ttggtgaaga agttgttgct cgttttgctg aagctggttt atttacattt cgtgaagatc      540

cagctgctcc aggtaaatat ttattagatt tagaaaaagc attattatta caagctcgtc      600

gtgctggttt acgtgaagaa cgtatttatc gtgttggttt atgtacacat tgtgctccaa      660

atttattttc acatcgtcgt gatcgtggtc gtacaggtcg tatgtggggt ttagttatgt      720

taccaccacg ttaagcatgc                                                  740


