                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Inc.
       Maddaloni, Massimo
       Pascual, David Wayne
       Ellis, James
 
<120>  BIOREMEDIATION OF XENOBIOTICS IN THE HONEY BEE HIVE

<130>  T17472; Thomas Horstemeyer Docket #: 222107-2230

<150>  62/750,396
<151>  2018-10-25

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  1035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DQ677027.1 Stenotrophomonas sp. YC-1 methyl parathion hydrolase 
       (mpd) gene

<400>  1
ctctagagat tgaattcata tgcccctgaa gaaccgcttg ctggcccgcc tgtcctgtgt       60

tgcggccgtg gtggccgcca cggccgccgt tgcaccgttg acgctggtgt ccaccgccca      120

cgccgccgca ccgcaggtgc gcacctcggc ccccggctac taccggatgc tgctgggcga      180

cttcgaaatc accgcgctgt cggacggcac ggtggcgctg ccggtcgaca agcggctgaa      240

ccagccggcc ccgaagacgc agagcgcgct ggccaagtcc ttccagaaag cgccgctcga      300

aacctcggtc accggttacc tcgtcaacac cggctccaag ctggtgctgg tggacaccgg      360

cgcggccggc ctgttcggcc ccaccctggg ccggctggcg gccaacctca aggccgcagg      420

ctatcagccc gagcaggtcg acgagatcta catcacccac atgcaccccg accacgtggg      480

cggcttgatg gtgggtgagc aactggcgtt cccgaacgcg gtggtgcgtg cggaccagaa      540

agaagccgat ttctggctca gccagaccaa cctcgacaag gccccggacg acgagagcaa      600

aggcttcttc aaaggcgcca tggcctcgct gaacccctat gtgaaggccg gcaagttcaa      660

gcctttctcg gggaacaccg acctggtgcc cggcatcaaa gcgctggcca gccacggcca      720

caccccgggc cacaccacct acgtggtcga aagccagggg caaaagctcg ccctgctcgg      780

cgacctgata ctcgtcgccg cggtgcagtt cgacgacccc agcgtcacga cccagctcga      840

cagcgacagc aagtccgtcg cggtggagcg caagaaggcc ttcgcggatg ccgccaaggg      900

cggctacctg atcgcggcgt cccacctgtc gttccccggc atcggccaca tccgcgccga      960

aggcaagggc taccgtttcg tgccggtgaa ctactcggtc gtcaacccca agtgactcga     1020

gaatcaatcg tcgac                                                      1035


<210>  2
<211>  1560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FJ688006.1 Sphingobium wenxiniae strain JZ-1 pyrethoid hydrolase 
       (pytH) gene

<400>  2
tgcggcctta tatcggcggc ggcctgaact ggacgatcat cttcaagacc gaagacggcg       60

tgctgaagga tttccgcacc agcaataccg tcggtcctgc gatcacgggc ggcatcgatg      120

tgccgctcaa cgagcggatc ggtgtgttcg gcgcggccag caaggtctgg accagcacca      180

acgcccgctt ccggctcggc acgccgaacg gcttcgtgcc gggtacggcg cgcgtccagc      240

tcaatcccct catcgcgcag gtcggcctgc aatatcgctt ctgatccccc cggctcgaac      300

aggaagacat gtcatgaccg tcaccgatat catcctgatc cacggcgcct tgaaccgcgg      360

cgcctgctat gacgcggtcg tcccgcttct cgaagcgcgc ggctaccgcg tccatgcgcc      420

cgacctgacc ggccatacgc ccggcgatgg cggccatttg tcggtcgtcg acatggagca      480

ttatacccgc ccagtcgctg acatcctggc acgggccgag gggcagtcga tccttctggg      540

gcacagcttg ggcggtgcat ccatctcgtg gctggcgcag caccatcccg acaaggtggc      600

cgggctgatc tacctgaccg cggtcctcac cgcgcccggt ataacgccgg aaaccttcgt      660

cctgcccggc gagcccaacc ggggcacgcc gcacgcgctg gacctgatcc agccggtcga      720

cgagggacgt gggctacagg cggatttctc gcgactggaa cggctccgcg aagttttcat      780

gggcgattat cccggcgagg gaatgccgcc tgccgaacag ttcatccaga cccagtcgac      840

cgtgcccttt ggcacgccca atccgatgga ggggcgcgcg ctggaaatcc cacgcctcta      900

tatcgaggcg ctggacgatg tcgtgattcc gatcgccgtg cagcgtcaga tgcagaagga      960

gttccccggt ccggtcgcgg tcgtgtcgct gccggccagc catgcgccct attactcgat     1020

gcccgaacgg ctggccgagg cgatcgccga tttcgccgat gccccggccg agtatcgcca     1080

gacggcgacg aaggctgggc ctgatcgacc agctggagcg gacgggggtc gagccgaccg     1140

agctgatcta ccgtgacggg ctgtccggtg atcgcctggc ggcccatgcg gtgcgcaagg     1200

gcggtgccta tcgcgatcgg ttcggcgcgc cctatctcgg catccaccgt gccgatctgc     1260

agacgacgct gggccgcgcg gtcggggcgg agcatattca tctggcgcac cggctggagc     1320

ggatcgagac ggtgggcgaa cgggtggcgc tccacttcgt gggacgcgat cccgtcgagg     1380

ccgatatcgt cgtgggtgcc gacggtgttc gctccatcac ccggcaatgg gtgggcgggc     1440

agccgctgcg ttattccggg acgagcgcgt ttcgcggcat cgtgccggtc gagcggctgg     1500

acgctttgcc cgatccccag gcgattcagt tctggatcgg ccaggacgcc catctgctgc     1560


<210>  3
<211>  570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AAB67728.1 E3 [Lucilia cuprina]

<400>  3

Met Asn Phe Asn Val Ser Leu Met Glu Lys Leu Lys Trp Lys Ile Lys 
1               5                   10                  15      


Cys Ile Glu Asn Lys Phe Leu Asn Tyr Arg Leu Thr Thr Asn Glu Thr 
            20                  25                  30          


Val Val Ala Glu Thr Glu Tyr Gly Lys Val Lys Gly Val Lys Arg Leu 
        35                  40                  45              


Thr Val Tyr Asp Asp Ser Tyr Tyr Ser Phe Glu Gly Ile Pro Tyr Ala 
    50                  55                  60                  


Gln Pro Pro Val Gly Glu Leu Arg Phe Lys Ala Pro Gln Arg Pro Thr 
65                  70                  75                  80  


Pro Trp Asp Gly Val Arg Asp Cys Cys Asn His Lys Asp Lys Ser Val 
                85                  90                  95      


Gln Val Asp Phe Ile Thr Gly Lys Val Cys Gly Ser Glu Asp Cys Leu 
            100                 105                 110         


Tyr Leu Ser Val Tyr Thr Asn Asn Leu Asn Pro Glu Thr Lys Arg Pro 
        115                 120                 125             


Val Leu Val Tyr Ile His Gly Gly Gly Phe Ile Ile Gly Glu Asn His 
    130                 135                 140                 


Arg Asp Met Tyr Gly Pro Asp Tyr Phe Ile Lys Lys Asp Val Val Leu 
145                 150                 155                 160 


Ile Asn Ile Gln Tyr Arg Leu Gly Ala Leu Gly Phe Leu Ser Leu Asn 
                165                 170                 175     


Ser Glu Asp Leu Asn Val Pro Gly Asn Ala Gly Leu Lys Asp Gln Val 
            180                 185                 190         


Met Ala Leu Arg Trp Ile Lys Asn Asn Cys Ala Asn Phe Gly Gly Asn 
        195                 200                 205             


Pro Asp Asn Ile Thr Val Phe Gly Glu Ser Ala Gly Ala Ala Ser Thr 
    210                 215                 220                 


His Tyr Met Met Leu Thr Glu Gln Thr Arg Gly Leu Phe His Arg Gly 
225                 230                 235                 240 


Ile Leu Met Ser Gly Asn Ala Ile Cys Pro Trp Ala Asn Thr Gln Cys 
                245                 250                 255     


Gln His Arg Ala Phe Thr Leu Ala Lys Leu Ala Gly Tyr Lys Gly Glu 
            260                 265                 270         


Asp Asn Asp Lys Asp Val Leu Glu Phe Leu Met Lys Ala Lys Pro Gln 
        275                 280                 285             


Asp Leu Ile Lys Leu Glu Glu Lys Val Leu Thr Leu Glu Glu Arg Thr 
    290                 295                 300                 


Asn Lys Val Met Phe Pro Phe Gly Pro Thr Val Glu Pro Tyr Gln Thr 
305                 310                 315                 320 


Ala Asp Cys Val Leu Pro Lys His Pro Arg Glu Met Val Lys Thr Ala 
                325                 330                 335     


Trp Gly Asn Ser Ile Pro Thr Met Met Gly Asn Thr Ser Tyr Glu Gly 
            340                 345                 350         


Leu Phe Phe Thr Ser Ile Leu Lys Gln Met Pro Met Leu Val Lys Glu 
        355                 360                 365             


Leu Glu Thr Cys Val Asn Phe Val Pro Ser Glu Leu Ala Asp Ala Glu 
    370                 375                 380                 


Arg Thr Ala Pro Glu Thr Leu Glu Met Gly Ala Lys Ile Lys Lys Ala 
385                 390                 395                 400 


His Val Thr Gly Glu Thr Pro Thr Ala Asp Asn Phe Met Asp Leu Cys 
                405                 410                 415     


Ser His Ile Tyr Phe Trp Phe Pro Met His Arg Leu Leu Gln Leu Arg 
            420                 425                 430         


Phe Asn His Thr Ser Gly Thr Pro Val Tyr Leu Tyr Arg Phe Asp Phe 
        435                 440                 445             


Asp Ser Glu Asp Leu Ile Asn Pro Tyr Arg Ile Met Arg Ser Gly Arg 
    450                 455                 460                 


Gly Val Lys Gly Val Ser His Ala Asp Glu Leu Thr Tyr Phe Phe Trp 
465                 470                 475                 480 


Asn Gln Leu Ala Lys Arg Met Pro Lys Glu Ser Arg Glu Tyr Lys Thr 
                485                 490                 495     


Ile Glu Arg Met Thr Gly Ile Trp Ile Gln Phe Ala Thr Thr Gly Asn 
            500                 505                 510         


Pro Tyr Ser Asn Glu Ile Glu Gly Met Glu Asn Val Ser Trp Asp Pro 
        515                 520                 525             


Ile Lys Lys Ser Asp Glu Val Tyr Lys Cys Leu Asn Ile Ser Asp Glu 
    530                 535                 540                 


Leu Lys Met Ile Asp Val Pro Glu Met Asp Lys Ile Lys Gln Trp Glu 
545                 550                 555                 560 


Ser Met Phe Glu Lys His Arg Asp Leu Phe 
                565                 570 


<210>  4
<211>  337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Deinococcus radiodurans phosphotriesterase, Genbank accession# 
       3GTF_A


<220>
<221>  misc_feature
<222>  (157)..(157)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

His His His His His His Gly Asp Ala Pro Gly Gly Ala His Met Thr 
1               5                   10                  15      


Ala Gln Thr Val Thr Gly Ala Val Ala Ala Ala Gln Leu Gly Ala Thr 
            20                  25                  30          


Leu Pro His Glu His Val Ile Phe Gly Tyr Pro Gly Tyr Ala Gly Asp 
        35                  40                  45              


Val Thr Leu Gly Pro Phe Asp His Ala Ala Ala Leu Ala Ser Cys Thr 
    50                  55                  60                  


Glu Thr Ala Arg Ala Leu Leu Ala Arg Gly Ile Gln Thr Val Val Asp 
65                  70                  75                  80  


Ala Thr Pro Asn Gly Cys Gly Arg Asn Pro Ala Phe Leu Arg Glu Val 
                85                  90                  95      


Ser Glu Ala Thr Gly Leu Gln Ile Leu Cys Ala Thr Gly Phe Tyr Tyr 
            100                 105                 110         


Glu Gly Gly Gly Ala Thr Thr Tyr Phe Lys Phe Arg Ala Ser Leu Gly 
        115                 120                 125             


Asp Ala Glu Ser Glu Ile Tyr Glu Met Met Arg Thr Glu Val Thr Glu 
    130                 135                 140                 


Gly Ile Ala Gly Thr Gly Ile Arg Ala Gly Val Ile Xaa Leu Ala Ser 
145                 150                 155                 160 


Ser Arg Asp Ala Ile Thr Pro Tyr Glu Gln Leu Phe Phe Arg Ala Ala 
                165                 170                 175     


Ala Arg Val Gln Arg Glu Thr Gly Val Pro Ile Ile Thr His Thr Gln 
            180                 185                 190         


Glu Gly Gln Gln Gly Pro Gln Gln Ala Glu Leu Leu Thr Ser Leu Gly 
        195                 200                 205             


Ala Asp Pro Ala Arg Ile Met Ile Gly His Met Asp Gly Asn Thr Asp 
    210                 215                 220                 


Pro Ala Tyr His Arg Glu Thr Leu Arg His Gly Val Ser Ile Ala Phe 
225                 230                 235                 240 


Asp Arg Ile Gly Leu Gln Gly Met Leu Gly Thr Pro Thr Asp Ala Glu 
                245                 250                 255     


Arg Leu Ser Val Leu Thr Thr Leu Leu Gly Glu Gly Tyr Ala Asp Arg 
            260                 265                 270         


Leu Leu Leu Ser His Asp Ser Ile Trp His Trp Leu Gly Arg Pro Pro 
        275                 280                 285             


Ala Ile Pro Glu Ala Ala Leu Pro Ala Val Lys Asp Trp His Pro Leu 
    290                 295                 300                 


His Ile Ser Asp Asp Ile Leu Pro Asp Leu Arg Arg Arg Gly Ile Thr 
305                 310                 315                 320 


Glu Glu Gln Val Gly Gln Met Thr Val Gly Asn Pro Ala Arg Leu Phe 
                325                 330                 335     


Gly 
    


<210>  5
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sulpholobus acidocaldarius phosphotriesterase, Genbank accession#
       WP_011278935

<400>  5

Met Thr Lys Ile Pro Leu Val Gly Lys Gly Glu Ile Ser Pro Gly Glu 
1               5                   10                  15      


Met Gly Phe Thr Leu Ile His Glu His Leu Arg Val Phe Ser Glu Pro 
            20                  25                  30          


Val Arg Tyr Gln Trp Pro His Leu Tyr Asn Glu Asp Glu Glu Leu Lys 
        35                  40                  45              


Asn Ala Val Asn Glu Val Lys Thr Ile Met Ser Tyr Gly Val Lys Thr 
    50                  55                  60                  


Ile Val Asp Pro Thr Val Met Gly Leu Gly Arg Asp Ile Arg Phe Ser 
65                  70                  75                  80  


Glu Lys Val Val Lys Glu Thr Gly Ile Asn Val Ile Ala Ala Thr Gly 
                85                  90                  95      


Leu Tyr Thr Tyr Thr Asp Leu Pro Phe Phe Phe Asn Gly Arg Ser Leu 
            100                 105                 110         


Glu Glu Ile Ala Glu Leu Leu Ile His Asp Ile Lys Lys Gly Ile Gln 
        115                 120                 125             


Gly Thr Asn Asn Arg Ala Gly Phe Ile Lys Val Ala Ala Asp Glu Pro 
    130                 135                 140                 


Gly Ile Thr Arg Asp Val Glu Arg Ala Ile Arg Ala Ala Ala Ile Ala 
145                 150                 155                 160 


Gln Lys Glu Thr Asn Val Pro Ile Ile Thr His Ser Asn Ala His Asn 
                165                 170                 175     


Gly Thr Gly Leu Glu Gln Gln Arg Ile Leu Met Glu Glu Gly Val Asp 
            180                 185                 190         


Pro Gly Arg Val Leu Ile Gly His Leu Gly Asp Thr Asp Asn Val Asp 
        195                 200                 205             


Tyr Ile Lys Lys Ile Ala Asp Lys Gly Ser Phe Val Gly Leu Asp Arg 
    210                 215                 220                 


Tyr Gly Leu Asp Leu Phe Leu Pro Ile Asp Lys Arg Asn Glu Val Leu 
225                 230                 235                 240 


Leu Lys Leu Ile Lys Asp Gly Tyr Leu Asp Arg Ile Met Val Ser Gln 
                245                 250                 255     


Asp Tyr Cys Cys Thr Ile Asp Trp Gly Ile Ala Lys Pro Glu Tyr Lys 
            260                 265                 270         


Pro Lys Leu Ala Pro Lys Trp Ser Met Ser Leu Ile Phe Thr Asp Val 
        275                 280                 285             


Ile Pro Ser Ile Lys Arg Ala Gly Val Thr Asp Glu Gln Leu His Val 
    290                 295                 300                 


Ile Phe Val Lys Asn Pro Ala Arg Leu Phe Ser 
305                 310                 315 


<210>  6
<211>  176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 6

<400>  6
gggccctatc gccggaaact taaaaaggtc aaggaagaaa cgaattattt atagaaagtc       60

tagcactcca aaacaaagct agacttttta tttaatattt tattgaaagc gctttcattt      120

ataacaaaag agtgctacta tatagacata gacaatagga ggaaaacaaa ggatcc          176


<210>  7
<211>  314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 7

<400>  7
gggccctgca ttaattttct tttttggtta aaataagcga aagaacctgg gcaacaaaac       60

tgtcaaaaat ttcatagaaa aacgctaaaa acccttgtct gacaagggtt tttctgttat      120

tatattcaag tacgcctttt ggggatgccg atgcaaaccc gctaaaaaga ctggtgtatc      180

aacgtttagc gatgatgatt aaggttgcga cacgcgcggc cgcgttgcca tgggcgcgca      240

aacacaacat gtgttgtcgg tttttgatcg gagttagctg atttacaaaa tcaacgagga      300

ggcagaaagg atcc                                                        314


<210>  8
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Burkholderia pseudomallei Oxalate decarboxylase protein

<400>  8

Met Thr Asn Leu Ser Arg Arg Lys Met Leu Ala Gly Thr Ala Gly Ala 
1               5                   10                  15      


Leu Ala Ala Ala Gly Ile Ala Val Ser Ala Lys Ala Ala Ser Phe Gly 
            20                  25                  30          


Asn Pro Asp Ser Pro Ala Glu Gly Ala Val Asn Ala Arg Asn Pro Gln 
        35                  40                  45              


Ser Leu Thr Asp Pro Gly Pro Gln Asn Pro Ala Leu Met Lys Glu Phe 
    50                  55                  60                  


Pro Ser Phe Gln Ser Pro Pro Ala Thr Asp Ile Asn Gly Met Pro Ile 
65                  70                  75                  80  


Phe Trp Ala Ser Phe Asn Asn Ala His Lys Arg Ile Gln Asn Gly Gly 
                85                  90                  95      


Trp Ala Arg Glu Val Thr Gln Glu Asp Phe Ala Ile Ser Glu Thr Ile 
            100                 105                 110         


Ser Gly Val Asn Met Arg Leu Ala Arg Gly Gly Ile Arg Glu Met His 
        115                 120                 125             


Trp His Gln Gln Ala Glu Trp Ala Phe Met Leu Asp Gly Arg Cys Arg 
    130                 135                 140                 


Ile Thr Val Leu Asp Glu Glu Gly Arg Pro Ser Val Gln Asp Val Lys 
145                 150                 155                 160 


Thr Gly Asp Leu Trp Tyr Phe Pro Pro Gly Leu Pro His Ser Leu Gln 
                165                 170                 175     


Gly Leu Gly Val Asp Gly Ala Glu Phe Leu Leu Ala Phe Asp Asn Gly 
            180                 185                 190         


Arg Ala Ser Glu Phe Asn Thr Leu Leu Val Thr Asp Trp Ile Ala His 
        195                 200                 205             


Thr Pro Pro Asp Val Leu Ala Leu Asn Phe Gly Val Pro Ala Asp Ala 
    210                 215                 220                 


Phe Arg Arg Ile Pro Leu Asp Asn Leu Trp Ile Phe Gln Gly Asp Asp 
225                 230                 235                 240 


Pro Gly Pro Leu Ala Ala Ala Gln Arg Ala Ser Ala Ser Ser Arg Gly 
                245                 250                 255     


Ala Pro Lys His Pro Phe Ile Phe Ser Met Gly Asp Met Lys Pro Asn 
            260                 265                 270         


Val Lys Thr Arg Gly Gly Glu Val Arg Ile Val Asp Ser Thr Asn Phe 
        275                 280                 285             


Ala Val Ser Lys Thr Ile Ala Ala Ala Leu Val Thr Val Lys Pro Gly 
    290                 295                 300                 


Gly Met Arg Glu Leu His Trp His Pro Asn Ala Asp Glu Trp Gln Tyr 
305                 310                 315                 320 


Tyr Ile Arg Gly Asp Ala Arg Met Thr Val Phe Asp Thr Gly Pro Lys 
                325                 330                 335     


Ala Gln Thr Ala Asp Phe Arg Ala Gly Asp Val Gly Tyr Val Lys Lys 
            340                 345                 350         


Ser Leu Gly His Tyr Val Gln Asn Thr Gly Thr Thr Asp Leu Val Phe 
        355                 360                 365             


Leu Glu Ile Phe Lys Ala Asp Arg Tyr Ala Glu Val Ser Leu Ser Asp 
    370                 375                 380                 


Trp Leu Ala His Thr Pro Pro Gln Leu Val Glu Ala His Leu His Ile 
385                 390                 395                 400 


Ala Pro Asp Val Ile Ala Arg Phe Pro Arg Asn Arg Pro Asp Val Val 
                405                 410                 415     


Pro Ala 
        


<210>  9
<211>  1257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Burkholderia pseudomallei Oxalate decarboxylase nucleic acid

<400>  9
atgacgaatc tgtcccgacg caagatgcta gccggcacgg ccggcgcgct cgccgccgcc       60

ggcatcgccg tttccgcgaa ggccgcttcg ttcggcaatc cggacagccc ggccgagggc      120

gcggtgaacg cgcgcaatcc gcaaagcctg accgatccgg ggccgcaaaa tccggcgctg      180

atgaaggagt ttccttcgtt tcagagtccg ccggccaccg acattaacgg catgccgatt      240

ttctgggcgt cgttcaataa tgcgcacaag cgcattcaga acggcggctg ggcgcgcgaa      300

gtcacgcagg aggatttcgc gatttccgaa acgatttccg gcgtcaacat gcggctcgcg      360

cgcggcggca ttcgcgagat gcactggcac cagcaggccg aatgggcgtt catgctcgac      420

ggccgctgcc ggatcaccgt gctcgatgaa gaggggcggc cgtccgtgca ggacgtgaag      480

acgggcgacc tctggtattt cccgccgggg ctgccgcatt cgctgcaggg gctcggcgtc      540

gacggcgccg aattcctgct cgcgttcgac aacggccgcg cgtccgaatt caacacgctg      600

ctcgtgacgg actggatcgc gcacacgccg cccgacgtgc tcgcgctgaa cttcggcgtg      660

cccgccgatg cattccgccg cattccgctc gacaatctgt ggatcttcca gggcgacgat      720

cccgggccgc tcgccgccgc gcagcgcgcg tcggcgtcgt cgcgcggcgc gccgaagcat      780

ccgttcatct tctcgatggg cgacatgaag ccgaacgtga agacgcgcgg cggcgaagtg      840

cggatcgtcg acagcacgaa cttcgctgtg tcgaagacga tcgcggccgc gctcgtcacg      900

gtgaagccgg gcggcatgcg cgagctgcac tggcacccga acgcggacga gtggcagtac      960

tacatccggg gcgacgcgcg catgacggtg ttcgacaccg gcccgaaggc gcagacggcc     1020

gatttccgcg cgggcgacgt cggctacgtg aagaagagcc tcggccacta cgtgcagaac     1080

acgggcacga ccgatctcgt gttcctcgag atcttcaagg cggaccgcta cgcggaggtt     1140

tcgctgtccg actggctcgc gcacacgccg ccgcagctcg tcgaggcgca tctgcatatc     1200

gcgcccgacg tgatcgcgcg ctttccgcgc aaccggccgg acgtcgtgcc ggcgtaa        1257


<210>  10
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pantoea allii oxalate decarboxylase protein

<400>  10

Met Ser Lys Leu Thr Arg Arg Ser Phe Met Ala Phe Ala Ala Gly Gly 
1               5                   10                  15      


Thr Val Ala Leu Ala Ala Gly Gln Val His Ala His Asp Ser Ile Gln 
            20                  25                  30          


Ser Phe Pro Thr Gly Gly Arg Asp Pro Gly Pro His Asp Pro Ile Arg 
        35                  40                  45              


Glu Ala Glu Asn Pro Asp Ile Val Asn Pro Pro Ser Thr Asp Ser Gly 
    50                  55                  60                  


Thr Leu Pro Asn Leu Arg Phe Ser Phe Ser Asp Ala His Val Arg Lys 
65                  70                  75                  80  


Ser Ser Gly Gly Trp Thr Arg Gln Val Thr Lys Arg Glu Leu Ala Ile 
                85                  90                  95      


Ser Thr Thr Ile Ala Gly Val Asp Met Arg Leu Asn Ala Gly Gly Ile 
            100                 105                 110         


Arg Glu Leu His Trp His Lys Glu Gly Glu Trp Ala Tyr Met Thr Tyr 
        115                 120                 125             


Gly Asn Ala Arg Val Thr Ala Phe Asp Thr Asp Gly Ser Trp Phe Ile 
    130                 135                 140                 


Asp Asp Ile Gly Pro Gly Asp Leu Trp Tyr Phe Pro Pro Gly Ile Pro 
145                 150                 155                 160 


His Ser Ile Gln Gly Ile Gly Pro Asp Gly Cys Glu Phe Leu Leu Ala 
                165                 170                 175     


Phe Asp Asp Gly Gly Phe Asp Glu Asp Ser Thr Phe Leu Leu Ser Asp 
            180                 185                 190         


Trp Phe Lys His Ile Pro Pro Asp Ile Leu Ala Lys Asn Phe Asn Val 
        195                 200                 205             


Pro Val Ser Thr Phe Asp His Leu Pro Ser Pro Ala Asp Glu Tyr Ile 
    210                 215                 220                 


Phe Ala Ala Lys Val Pro Gly Ser Leu Glu Ser Val Ala Val Lys Gly 
225                 230                 235                 240 


Ala Thr Lys Ala Lys Arg Thr Phe Thr His His Met Leu Ala Gln Glu 
                245                 250                 255     


Pro Ile Lys Ala Pro Gly Gly Thr Val Arg Ile Thr Asp Ser Ser Thr 
            260                 265                 270         


Phe Lys Val Ser Lys Thr Ile Ala Ala Ala Leu Val Glu Ile Ala Pro 
        275                 280                 285             


Gly Gly Leu Arg Glu Leu His Trp His Pro Asn Asn Asp Glu Trp Gln 
    290                 295                 300                 


Tyr Tyr Leu Glu Gly Glu Gly Arg Met Gly Val Phe Ala Ser Ser Gly 
305                 310                 315                 320 


Gln Ala Arg Thr Phe Asp Tyr Arg Ala Gly Asp Val Gly Tyr Val Pro 
                325                 330                 335     


Phe Ala Met Gly His Tyr Val Glu Asn Thr Gly Lys Gly Pro Leu Arg 
            340                 345                 350         


Phe Leu Glu Leu Phe Lys Ser Asp Tyr Tyr Ala Asp Ile Ser Leu Asn 
        355                 360                 365             


Gln Trp Leu Ala Gly Thr Pro Arg Glu Leu Val Lys Ala His Leu Gln 
    370                 375                 380                 


Leu Asp Asp Ala Phe Met Asp Ala Leu Ser Leu Gln Lys Asn Pro Val 
385                 390                 395                 400 


Val Lys 
        


<210>  11
<211>  1209
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pantoea allii oxalate decarboxylase nucleic acid

<400>  11
atgagtaaat taacccgaag aagttttatg gcgtttgccg caggcgggac ggtggcgctg       60

gcggctggac aggttcatgc gcatgacagc attcagtcct ttccgaccgg tggtcgcgat      120

cccgggccgc acgatcccat ccgcgaagca gaaaatccgg acattgttaa tccaccttcg      180

accgacagcg ggacgttacc gaacctgcgt ttttcattca gtgatgcgca cgtgcgcaaa      240

agcagcggcg gatggacgcg ccaggtcacc aagcgtgaat tagccatttc gaccaccatt      300

gccggtgtgg acatgcgcct gaatgccggc ggcatccgcg aattacactg gcataaagag      360

ggcgaatggg cctacatgac ctatggcaac gcccgcgtca ccgcgtttga taccgatggc      420

agttggttta tcgatgatat tggcccggga gacttatggt atttcccgcc gggcatcccg      480

cactcaattc agggcatcgg gcctgatgga tgcgaatttc tgctggcctt cgacgatggc      540

ggatttgatg aggacagcac gtttctgtta tccgactggt tcaaacatat cccgccggac      600

attctggcga aaaacttcaa cgtcccggta tccaccttcg accacctgcc ttcgccagcg      660

gatgaatata tttttgccgc gaaggtgccc ggcagcctgg agtcagtggc ggtgaaaggc      720

gcaaccaaag ccaaacgcac ctttacccac cacatgctgg cacaggaacc cataaaggca      780

ccgggcggca cggtcagaat caccgactcc tcaaccttta aggtgtccaa aactatcgcg      840

gcagccctgg ttgaaatcgc ccccggcgga ttacgcgagt tgcactggca cccgaataac      900

gatgaatggc aatactacct ggaaggggaa ggacgcatgg gcgtttttgc ctcatcgggt      960

caggcaagaa catttgatta tcgggcaggg gatgtcggct atgtgccctt cgccatgggc     1020

cactatgttg aaaacaccgg caaggggccg ctgcgtttcc tggagttatt caaaagtgac     1080

tattacgccg atatctcact caatcagtgg ctggccggta cgccgcgtga gctggtaaaa     1140

gcgcatctcc agttagatga cgcatttatg gatgcgctgt cgctgcagaa aaatcctgtg     1200

gtgaaataa                                                             1209


<210>  12
<211>  2688
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette expressing transporter and cch2 (6-CNA Chlorohydrolase; 
       including HindIII and BamHI cloning ends) (nucleic acid)

<400>  12
aagcttagga ggtctagaaa tatgatgcga cgtgtacttg tccttggtat ctttatgatt       60

actcaatctg tagttgcaga agctgcagat gaaatcaaaa ttggtgaaat caactcttat      120

tctacagcag ctagttttac tgaaccatat cgtaaaggtt ggcaacttgc agtagatgaa      180

atcaatgcta aaggaggtgt tcttggcaag aaacttatcg ttattgcaaa ggacgatgct      240

ggaaaacctg ctgatgcgat tactgctgcg aatgatctta tggcaaacga aggtgtcgtt      300

atgttagctg gtacatttct ttctcatgta gctctcgcag tatcagattt cgccaaacag      360

aaaaagatcg tgtttatggc tggtgaacct ttgacagatg cacttacgtg gtcacaaggt      420

aataagtaca cattccgtct tcgcccaaat acctatatgc aagctgcaat gttggctgaa      480

gaggcagcga aactgccagc aaaacgttgg gctaccattg ctccgaacta tgagtttgga      540

caatctgcag ttgcagcttt caagaaactt ttgtctcaaa aacgtccaga tgttgaatgg      600

gtaggagaac aatggccacc tctcggcaag attgatgctg gaccagtagt tcaagcactt      660

gctgcaacta aaccagatgc aattatgaat gcagtatttg gagttgatct cgccaaattt      720

gtacgtgaag gtaatgcacg tgctctgttc aaagatagat cagttgtaag tttcttgaca      780

ggtgaaccag agtttctcga tcctctgaaa gacgattcac ctgttggctg gattgtgaca      840

ggttatcctt ggtatggaat caaaactcca gaacatgatg cgtttctcaa agcatatcaa      900

gcaaaacata aagattatcc tcgtcttggt tctgtagttg gctatcaacc cattatggct      960

gcagccgcta tgcttaacaa agcaggatca actgatactg ataaacttat cgcagcgggt     1020

gaagatttga agatggaatc accatttgga ccaatcgtac tccgtaaaat tgatcatcaa     1080

gctacattag gagattatat cggtaaaatc gctattaagg atggtatggg agtgatggta     1140

gacttcaaat actttgatgg agctcaatat ctacctacag atgcagaaat cggaaaaatg     1200

cgtccaaagg attaaatacg ttcactaagg agggtctcta gaatgagtct tattgcaatc     1260

aaaaatgcag attggattct tactatggat ggagctcgtc gagttattgc agatggatct     1320

atcttgattg acaaagatcg tattcgagca attggtaaat ctgctgtgat tgaaatccct     1380

ccagaaacaa ctaaggttat tgatggtcgt ggaaaagttg tattaccagg attgattgat     1440

tctcatacac accattcact tcatttaggt cgtggacttg cagatgaatg tgacattcaa     1500

acgtttctgt atcgacgttt gtatcccatt gaagcatcac ttaatgatga agatgcttac     1560

attagtgcat tgttatgtca acttgaaatg attaagtctg gtactacatc gattatcgat     1620

gctggaaact actttcctga agcaacgctt cgtgcatttg gaacaactgg tatgcgtgga     1680

gttgtagcac gaagtacgtt tgatattcca acatcttcac ttggatcatt gccagcacaa     1740

gtgtttgctg aagagaccaa tgttgcattg aaacgtgctg aagagtttgt agaacgaaac     1800

agtggtgctt gtgatggtcg tgtgcaagca tggttacaat tgcgtgttct tccgaattgt     1860

tctgatgaac tttgccgtgg cttaaaaagt attgctgatc gacttggagt tcgttatgaa     1920

gcacatgcag ccttcactaa agaagtctat gaagcctcta aactgcagtt tggtaaatct     1980

gaagttcgtc gcttggatga cctcggtatc ctcggtgaag gattattgct cgcgcatatg     2040

ggatggttaa cgccacgtga catcttgtta ctcattagtt ctaaaactaa tgttgttctt     2100

tgtccaacat ctagtgtaca tcaagcgatg ggtagcattg catttggaca tgtccctgag     2160

ttgttagaaa tgggagttaa cgttgctctt ggaacggatg gcggtcctca tggaactaat     2220

gatcttgttc gccaaatctt tgtagctgca ggaggctata aggaagttcg tcttgatgca     2280

acaatcatgc cacctgagac agtacttgaa atggcaaccg ttaacggtgc tcgtgcaatg     2340

ggaatgtcag atcaagtagg atctattgaa cctggtaaga aagcagacat tacaatcttt     2400

gattctcgtc gacctgaatg gcgtccattg cacaatccag tcgcgaatct cgtatattgc     2460

gcaaacggaa attctgctga taccgttatt gtagatggac gtatcttgat ggagaatcgt     2520

aaaattctca ccttcaacga agatgacgta attactgaag ctcaacgtcg atcagttgag     2580

attggtgctc gtgcaggatt gttagaatat ggacgaccac gctggccagt ccattgaaga     2640

gatataaaaa gccagattat taatccggct tttttattat ttggatcc                  2688


<210>  13
<211>  841
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cassette expressing transporter and cch2 (6-CNA Chlorohydrolase) 
       (protein)

<400>  13

Met Met Arg Arg Val Leu Val Leu Gly Ile Phe Met Ile Thr Gln Ser 
1               5                   10                  15      


Val Val Ala Glu Ala Ala Asp Glu Ile Lys Ile Gly Glu Ile Asn Ser 
            20                  25                  30          


Tyr Ser Thr Ala Ala Ser Phe Thr Glu Pro Tyr Arg Lys Gly Trp Gln 
        35                  40                  45              


Leu Ala Val Asp Glu Ile Asn Ala Lys Gly Gly Val Leu Gly Lys Lys 
    50                  55                  60                  


Leu Ile Val Ile Ala Lys Asp Asp Ala Gly Lys Pro Ala Asp Ala Ile 
65                  70                  75                  80  


Thr Ala Ala Asn Asp Leu Met Ala Asn Glu Gly Val Val Met Leu Ala 
                85                  90                  95      


Gly Thr Phe Leu Ser His Val Ala Leu Ala Val Ser Asp Phe Ala Lys 
            100                 105                 110         


Gln Lys Lys Ile Val Phe Met Ala Gly Glu Pro Leu Thr Asp Ala Leu 
        115                 120                 125             


Thr Trp Ser Gln Gly Asn Lys Tyr Thr Phe Arg Leu Arg Pro Asn Thr 
    130                 135                 140                 


Tyr Met Gln Ala Ala Met Leu Ala Glu Glu Ala Ala Lys Leu Pro Ala 
145                 150                 155                 160 


Lys Arg Trp Ala Thr Ile Ala Pro Asn Tyr Glu Phe Gly Gln Ser Ala 
                165                 170                 175     


Val Ala Ala Phe Lys Lys Leu Leu Ser Gln Lys Arg Pro Asp Val Glu 
            180                 185                 190         


Trp Val Gly Glu Gln Trp Pro Pro Leu Gly Lys Ile Asp Ala Gly Pro 
        195                 200                 205             


Val Val Gln Ala Leu Ala Ala Thr Lys Pro Asp Ala Ile Met Asn Ala 
    210                 215                 220                 


Val Phe Gly Val Asp Leu Ala Lys Phe Val Arg Glu Gly Asn Ala Arg 
225                 230                 235                 240 


Ala Leu Phe Lys Asp Arg Ser Val Val Ser Phe Leu Thr Gly Glu Pro 
                245                 250                 255     


Glu Phe Leu Asp Pro Leu Lys Asp Asp Ser Pro Val Gly Trp Ile Val 
            260                 265                 270         


Thr Gly Tyr Pro Trp Tyr Gly Ile Lys Thr Pro Glu His Asp Ala Phe 
        275                 280                 285             


Leu Lys Ala Tyr Gln Ala Lys His Lys Asp Tyr Pro Arg Leu Gly Ser 
    290                 295                 300                 


Val Val Gly Tyr Gln Pro Ile Met Ala Ala Ala Ala Met Leu Asn Lys 
305                 310                 315                 320 


Ala Gly Ser Thr Asp Thr Asp Lys Leu Ile Ala Ala Gly Glu Asp Leu 
                325                 330                 335     


Lys Met Glu Ser Pro Phe Gly Pro Ile Val Leu Arg Lys Ile Asp His 
            340                 345                 350         


Gln Ala Thr Leu Gly Asp Tyr Ile Gly Lys Ile Ala Ile Lys Asp Gly 
        355                 360                 365             


Met Gly Val Met Val Asp Phe Lys Tyr Phe Asp Gly Ala Gln Tyr Leu 
    370                 375                 380                 


Pro Thr Asp Ala Glu Ile Gly Lys Met Arg Pro Lys Asp Met Ser Leu 
385                 390                 395                 400 


Ile Ala Ile Lys Asn Ala Asp Trp Ile Leu Thr Met Asp Gly Ala Arg 
                405                 410                 415     


Arg Val Ile Ala Asp Gly Ser Ile Leu Ile Asp Lys Asp Arg Ile Arg 
            420                 425                 430         


Ala Ile Gly Lys Ser Ala Val Ile Glu Ile Pro Pro Glu Thr Thr Lys 
        435                 440                 445             


Val Ile Asp Gly Arg Gly Lys Val Val Leu Pro Gly Leu Ile Asp Ser 
    450                 455                 460                 


His Thr His His Ser Leu His Leu Gly Arg Gly Leu Ala Asp Glu Cys 
465                 470                 475                 480 


Asp Ile Gln Thr Phe Leu Tyr Arg Arg Leu Tyr Pro Ile Glu Ala Ser 
                485                 490                 495     


Leu Asn Asp Glu Asp Ala Tyr Ile Ser Ala Leu Leu Cys Gln Leu Glu 
            500                 505                 510         


Met Ile Lys Ser Gly Thr Thr Ser Ile Ile Asp Ala Gly Asn Tyr Phe 
        515                 520                 525             


Pro Glu Ala Thr Leu Arg Ala Phe Gly Thr Thr Gly Met Arg Gly Val 
    530                 535                 540                 


Val Ala Arg Ser Thr Phe Asp Ile Pro Thr Ser Ser Leu Gly Ser Leu 
545                 550                 555                 560 


Pro Ala Gln Val Phe Ala Glu Glu Thr Asn Val Ala Leu Lys Arg Ala 
                565                 570                 575     


Glu Glu Phe Val Glu Arg Asn Ser Gly Ala Cys Asp Gly Arg Val Gln 
            580                 585                 590         


Ala Trp Leu Gln Leu Arg Val Leu Pro Asn Cys Ser Asp Glu Leu Cys 
        595                 600                 605             


Arg Gly Leu Lys Ser Ile Ala Asp Arg Leu Gly Val Arg Tyr Glu Ala 
    610                 615                 620                 


His Ala Ala Phe Thr Lys Glu Val Tyr Glu Ala Ser Lys Leu Gln Phe 
625                 630                 635                 640 


Gly Lys Ser Glu Val Arg Arg Leu Asp Asp Leu Gly Ile Leu Gly Glu 
                645                 650                 655     


Gly Lys Thr Asn Val Val Leu Cys Pro Thr Ser Ser Val His Gln Ala 
            660                 665                 670         


Met Gly Ser Ile Ala Phe Gly His Val Pro Glu Leu Leu Glu Met Gly 
        675                 680                 685             


Val Asn Val Ala Leu Gly Thr Asp Gly Gly Pro His Gly Thr Asn Asp 
    690                 695                 700                 


Leu Val Arg Gln Ile Phe Val Ala Ala Gly Gly Tyr Lys Glu Val Arg 
705                 710                 715                 720 


Leu Asp Ala Thr Ile Met Pro Pro Glu Thr Val Leu Glu Met Ala Thr 
                725                 730                 735     


Val Asn Gly Ala Arg Ala Met Gly Met Ser Asp Gln Val Gly Ser Ile 
            740                 745                 750         


Glu Pro Gly Lys Lys Ala Asp Ile Thr Ile Phe Asp Ser Arg Arg Pro 
        755                 760                 765             


Glu Trp Arg Pro Leu His Asn Pro Val Ala Asn Leu Val Tyr Cys Ala 
    770                 775                 780                 


Asn Gly Asn Ser Ala Asp Thr Val Ile Val Asp Gly Arg Ile Leu Met 
785                 790                 795                 800 


Glu Asn Arg Lys Ile Leu Thr Phe Asn Glu Asp Asp Val Ile Thr Glu 
                805                 810                 815     


Ala Gln Arg Arg Ser Val Glu Ile Gly Ala Arg Ala Gly Leu Leu Glu 
            820                 825                 830         


Tyr Gly Arg Pro Arg Trp Pro Val His 
        835                 840     


<210>  14
<211>  430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  porin-like protein, nicP (NCBI accession NP_746069.1)

<400>  14

Met Gln Gly Phe Thr Ala Pro Arg Phe Ser Ser Ser Ala Arg Leu Ala 
1               5                   10                  15      


Gly Gly Cys Ile Ala Ala Ala Leu Cys Ala Glu Ala Thr Ala Glu Glu 
            20                  25                  30          


Ala Gly Phe Ile Glu Gly Ala Arg Ala Thr Leu Gln Ala Arg Asn Tyr 
        35                  40                  45              


Phe Phe Ser Arg Asp Tyr Ala Asp Ile Lys Gly Ala Ser Thr Gln Ser 
    50                  55                  60                  


Arg Thr Gln Glu Trp Ala Gln Gly Phe Ile Leu Asn Ala Ser Ser Gly 
65                  70                  75                  80  


Tyr Thr Gln Gly Thr Leu Gly Val Gly Val Asp Val Thr Gly Leu Leu 
                85                  90                  95      


Gly Phe Lys Leu Asp Ser Ser Pro Glu His Ala Arg Ser Gly Leu Leu 
            100                 105                 110         


Pro Ser Leu Asp Ser Gly Lys Ser Ala Asp Glu Tyr Ser Arg Leu Gly 
        115                 120                 125             


Ala Ala Ile Lys Phe Lys Val Ser Gln Thr Glu Leu Lys Val Gly Glu 
    130                 135                 140                 


Leu Met Pro Asn Leu Pro Val Leu Leu Phe Ser Asp Leu Arg Leu Leu 
145                 150                 155                 160 


Pro Pro Thr Tyr Gln Gly Ala Met Leu Glu Ser Arg Glu Phe Ala Gly 
                165                 170                 175     


Leu Thr Leu Ser Ala Gly Gln Phe Arg Ser Thr Ser Leu Arg Asp Ser 
            180                 185                 190         


Ser Asn Ser Gln Lys Met Tyr Ala Leu Val Asn Asp Pro Ile Asn Pro 
        195                 200                 205             


Ala Arg Leu Ala Arg Phe Thr Ser Asp Arg Phe Asn Tyr Val Gly Ala 
    210                 215                 220                 


Asp Tyr Ala Phe Asn Asp Asn Arg Thr Ser Val Gly Val Trp Gln Ala 
225                 230                 235                 240 


Gln Leu Glu Asp Ile Tyr Gln Gln Arg Phe Tyr Ser Phe Lys His Ala 
                245                 250                 255     


Glu Pro Leu Gly Ser Trp Thr Leu Gly Val Asn Ala Gly Tyr Phe Asp 
            260                 265                 270         


Ala Arg Glu Asp Gly Ser Lys Val Ala Gly Asp Tyr Asp Asn His Ala 
        275                 280                 285             


Leu Phe Ser Leu Phe Ser Ala Lys Thr Gly Gly His Thr Phe Tyr Val 
    290                 295                 300                 


Gly Tyr Gln Gln Ile Gly Gly Asp Asp Gly Phe Ile Gln Val Gly Ala 
305                 310                 315                 320 


Asn Thr Asn Pro Met Gly Asn Thr Leu Pro Thr Tyr Glu Phe Ser Ala 
                325                 330                 335     


Pro Gly Glu Arg Ser Trp Gln Val Arg His Asp Tyr Asn Phe Val Ala 
            340                 345                 350         


Leu Gly Leu Pro Gly Leu Thr Ser Thr Leu Arg Tyr Val Lys Gly Arg 
        355                 360                 365             


Asp Val Glu Thr Gly Leu Gly Phe Glu Gly Arg Asp Arg Glu Arg Asp 
    370                 375                 380                 


Leu Asp Leu Ala Tyr Val Val Gln Ser Gly Pro Leu Ala Gly Leu Gly 
385                 390                 395                 400 


Ile Arg Val Arg Asn Val Met Ala Arg Ser Asn Tyr Arg Thr Asp Ile 
                405                 410                 415     


Asp Glu Asn Arg Leu Ile Leu Ser Tyr Thr Ile Lys Val Phe 
            420                 425                 430 


<210>  15
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  metabolite transport protein, NicT (NCBI accession NP_746070.1)

<400>  15

Met Pro Ile Ala Asn Ala Thr Thr Val His Ser Asp Ile Asp His Gly 
1               5                   10                  15      


Thr Lys Ala Leu Tyr Ser Lys Ile Thr Trp Arg Leu Ile Pro Phe Leu 
            20                  25                  30          


Cys Phe Cys Tyr Leu Ala Ala Tyr Leu Asp Arg Ile Asn Val Gly Phe 
        35                  40                  45              


Ala Lys Leu Gln Met Leu Glu Asp Leu Gln Phe Ser Thr Ala Ala Tyr 
    50                  55                  60                  


Gly Leu Gly Ala Gly Leu Phe Phe Val Gly Tyr Ile Ile Phe Glu Val 
65                  70                  75                  80  


Pro Ser Asn Leu Ile Leu Gln Arg Val Gly Ala Lys Leu Trp Ile Ala 
                85                  90                  95      


Arg Ile Met Ile Thr Trp Gly Leu Leu Ser Ala Cys Thr Met Phe Val 
            100                 105                 110         


Thr Ser Thr Thr Gln Phe Tyr Ile Leu Arg Phe Leu Leu Gly Ala Ala 
        115                 120                 125             


Glu Ala Gly Phe Leu Pro Gly Val Leu Tyr Tyr Leu Thr Met Trp Tyr 
    130                 135                 140                 


Pro Thr Tyr Arg Arg Gly Arg Ile Ile Ala Leu Phe Met Ile Gly Leu 
145                 150                 155                 160 


Pro Leu Ser Ser Val Ile Gly Gly Pro Ile Ser Gly Trp Ile Met Gly 
                165                 170                 175     


His Phe Asp Gln Val Gln Gly Leu His Gly Trp Gln Trp Leu Phe Leu 
            180                 185                 190         


Leu Glu Ala Ile Pro Ser Val Leu Leu Gly Ile Leu Thr Phe Trp Ala 
        195                 200                 205             


Leu Pro Asn His Phe Gln Gln Ala Lys Trp Leu Ser Ala Asp Asp Lys 
    210                 215                 220                 


Ala Gln Leu Ala Ala Asp Leu Ala Ala Asp Asp Ala Glu Gly Lys Asp 
225                 230                 235                 240 


Ser Lys His Ser Phe Arg Asp Gly Phe Phe Asn Leu Lys Val Trp Met 
                245                 250                 255     


Leu Gly Gly Ile Asp Phe Ser Ile Leu Leu Ser Ala Tyr Ala Met Gly 
            260                 265                 270         


Phe Trp Met Pro Thr Phe Ile Arg Asp Ala Gly Val Ser Asp Thr Phe 
        275                 280                 285             


His Ile Gly Leu Leu Thr Ala Ile Pro Ser Leu Ala Ala Leu Ala Gly 
    290                 295                 300                 


Met Leu Met Ile Gly Ala Ser Ser Asp Arg His Arg Glu Arg Arg Trp 
305                 310                 315                 320 


His Ile Ile Val Pro Phe Ile Ile Gly Ala Ile Ala Met Ala Ser Ser 
                325                 330                 335     


Thr Leu Phe Ser Gln Asn Leu Val Met Thr Val Val Leu Phe Ala Ile 
            340                 345                 350         


Ala Ser Ala Ala Ile Ile Gly Ala Val Pro Val Phe Phe Ser Leu Pro 
        355                 360                 365             


Ala Thr Phe Leu Lys Gly Thr Ala Ala Ala Thr Gly Phe Ala Leu Ala 
    370                 375                 380                 


Cys Ser Val Ala Asn Ile Ala Gly Leu Val Ser Asn Ser Leu Met Gly 
385                 390                 395                 400 


Val Val Thr Asp Leu Thr Gly Thr Ser His Ala Ala Leu Trp Val Phe 
                405                 410                 415     


Ala Gly Cys Leu Ile Leu Ser Cys Phe Leu Val Ile Ala Leu Pro Ala 
            420                 425                 430         


Lys Leu Val Asn Arg 
        435         


<210>  16
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  maleamate amidohydrolase, NicF (NCBI accession NP_746071.1)

<400>  16

Met Ser Asp Ala Gln Ser Ala Arg Asp Asn Tyr Gln Gly Val Trp Gly 
1               5                   10                  15      


Gln Arg Ile Gly Phe Gly Arg Lys Pro Ala Leu Leu Met Ile Asp Phe 
            20                  25                  30          


Met Gln Gly Tyr Thr Thr Pro Gly Ala Pro Leu Tyr Ala Pro Gly Val 
        35                  40                  45              


Val Ala Ala Val Glu Gln Ala Ala Gly Leu Leu Ala Leu Ala Arg Asp 
    50                  55                  60                  


Cys Gly Thr Leu Val Val His Thr Asn Ile Arg Tyr Gln Pro Pro His 
65                  70                  75                  80  


Phe Ala Asp Gly Gly Val Trp Val Arg Lys Ala Pro Val Met Lys Asp 
                85                  90                  95      


Met Val Glu Gly Asn Pro Leu Ala Ala Phe Cys Glu Ala Val Ala Pro 
            100                 105                 110         


Gln Ala Gly Glu Val Val Leu Ser Lys Gln Tyr Ala Ser Ala Phe Phe 
        115                 120                 125             


Ala Thr Ser Leu Ala Pro Leu Leu His Ala Gln Gly Val Asp Thr Val 
    130                 135                 140                 


Val Leu Ala Gly Cys Ser Thr Ser Gly Cys Ile Arg Ala Ser Ala Val 
145                 150                 155                 160 


Asp Ala Met Gln His Gly Phe Arg Thr Ile Val Val Arg Glu Cys Val 
                165                 170                 175     


Gly Asp Arg His Ser Asp Pro His Glu Ala Asn Leu Phe Asp Ile Asp 
            180                 185                 190         


Ser Lys Tyr Gly Asp Val Val Thr Arg Gln Asp Ala Met Gln Gln Leu 
        195                 200                 205             


Arg His Leu Ala Gly 
    210             


<210>  17
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  maleate isomerase, NicE (NCBI accession NP_746072.1)

<400>  17

Met Thr Gln Leu Tyr Arg Ile Gly Gln Ile Val Pro Ser Ser Asn Thr 
1               5                   10                  15      


Thr Met Glu Thr Glu Ile Pro Ala Met Leu Asn Ala Arg Gln Ala Ile 
            20                  25                  30          


Arg Pro Glu Arg Phe Thr Phe His Ser Ser Arg Met Arg Met Lys Gln 
        35                  40                  45              


Val Lys Lys Glu Glu Leu Ala Ala Met Asp Ala Glu Ser Asp Arg Cys 
    50                  55                  60                  


Ala Val Glu Leu Ser Asp Ala Lys Val Asp Val Leu Gly Tyr Ala Cys 
65                  70                  75                  80  


Leu Val Ala Ile Met Ala Met Gly Leu Gly Tyr His Arg Gln Ser Glu 
                85                  90                  95      


Lys Arg Leu Gln Gln Ala Thr Ala Asp Asn Asp Ala Leu Ala Pro Val 
            100                 105                 110         


Ile Thr Ser Ala Gly Ala Leu Val Glu Ala Leu His Val Met Lys Ala 
        115                 120                 125             


Lys Arg Ile Ala Ile Val Ala Pro Tyr Met Lys Pro Leu Thr Glu Leu 
    130                 135                 140                 


Val Val Asn Tyr Ile Arg Glu Glu Gly Phe Glu Val Gln Asp Trp Arg 
145                 150                 155                 160 


Ala Leu Glu Ile Pro Asp Asn Leu Ala Val Ala Arg His Asp Pro Ala 
                165                 170                 175     


Asn Leu Pro Gly Ile Val Ala Gly Met Asp Leu Glu Gly Val Asp Val 
            180                 185                 190         


Val Val Leu Ser Ala Cys Val Gln Met Gln Ser Leu Pro Ala Val Ala 
        195                 200                 205             


Lys Val Glu Ala Gln Thr Gly Lys Pro Val Val Thr Ala Ala Ile Ala 
    210                 215                 220                 


Thr Thr Tyr Ala Met Leu Lys Ala Leu Asp Leu Glu Pro Ile Val Pro 
225                 230                 235                 240 


Gly Ala Gly Ala Leu Leu Ser Gly Ala Tyr 
                245                 250 


<210>  18
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-formylmaleamate deformylase, NicD (NCBI accession NP_746073.1)

<400>  18

Met Ser Thr Phe Val Ala Gly Gly Asn Val Ser Ala Asn Gly Ile Arg 
1               5                   10                  15      


Gln His Tyr Leu Arg Tyr Gly Gly Lys Gly His Ala Leu Ile Leu Val 
            20                  25                  30          


Pro Gly Ile Thr Ser Pro Ala Ile Thr Trp Gly Phe Val Ala Glu Arg 
        35                  40                  45              


Leu Gly His Tyr Phe Asp Thr Tyr Val Leu Asp Val Arg Gly Arg Gly 
    50                  55                  60                  


Leu Ser Ser Ser Gly Pro Asp Leu Asp Tyr Gly Thr Asp Ala Cys Ala 
65                  70                  75                  80  


Ala Asp Ile Pro Ala Phe Ala Ala Ala Leu Gly Leu Asp Ser Tyr His 
                85                  90                  95      


Leu Leu Gly His Ser Met Gly Ala Arg Phe Ala Ile Arg Ala Ala Ala 
            100                 105                 110         


Gln Gly Ala Pro Gly Leu Gln Arg Leu Val Leu Val Asp Pro Pro Val 
        115                 120                 125             


Ser Gly Pro Gly Arg Arg Ala Tyr Pro Ser Lys Leu Pro Trp Tyr Val 
    130                 135                 140                 


Asp Ser Ile Arg Gln Ala Thr Val Gly Met Ser Gly Asp Asp Met Arg 
145                 150                 155                 160 


Ala Phe Cys Ala Thr Trp Ser Asp Glu Gln Leu Ala Leu Arg Ala Glu 
                165                 170                 175     


Trp Leu His Thr Cys Tyr Glu Pro Ala Ile Val Arg Ala Phe Asp Asp 
            180                 185                 190         


Phe His Glu Val Asp Ile His Gln Tyr Leu Pro Ala Val Arg Gln Pro 
        195                 200                 205             


Ala Leu Leu Met Val Ala Gly Arg Gly Gly Val Ile Glu Pro Arg Asp 
    210                 215                 220                 


Ile Ala Glu Met Arg Glu Leu Lys Pro Asp Ile Gln Val Ala Tyr Val 
225                 230                 235                 240 


Asp Asn Ala Gly His Met Ile Pro Trp Asp Asp Leu Asp Gly Phe Phe 
                245                 250                 255     


Ala Ala Phe Gly Asp Phe Leu Asp His Pro Leu Val 
            260                 265             


<210>  19
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-hydroxynicotinate 3-monooxygenase, NicC (NCBI accession 
       NP_746074.1)

<400>  19

Met Arg Gly Arg Gln Lys Ile Ala Ile Val Gly Ala Gly Leu Gly Gly 
1               5                   10                  15      


Ala Ala Ala Ala Thr Leu Leu Gln Gln Ala Gly Phe Asp Val Glu Val 
            20                  25                  30          


Phe Glu Gln Ala Pro Ala Phe Thr Arg Leu Gly Ala Gly Ile His Ile 
        35                  40                  45              


Gly Pro Asn Val Met Lys Ile Phe Arg Arg Met Gly Leu Glu Gln Lys 
    50                  55                  60                  


Leu Glu Leu Met Gly Ser His Pro Asp Phe Trp Phe Ser Arg Asp Gly 
65                  70                  75                  80  


Asn Thr Gly Asp Tyr Leu Ser Arg Ile Pro Leu Gly Glu Phe Ala Arg 
                85                  90                  95      


Arg Glu Tyr Gly Ala Ala Tyr Ile Thr Ile His Arg Gly Asp Leu His 
            100                 105                 110         


Ala Leu Gln Ile Glu Ala Ile Gln Pro Gly Thr Val His Phe Gly Lys 
        115                 120                 125             


Arg Leu Glu Lys Ile Val Asp Glu Gly Asp Gln Val Arg Leu Asp Phe 
    130                 135                 140                 


Ala Asp Gly Thr His Thr Val Ala Asp Ile Val Ile Gly Ala Asp Gly 
145                 150                 155                 160 


Ile His Ser Lys Ile Arg Glu Glu Leu Leu Gly Ala Glu Ala Pro Ile 
                165                 170                 175     


Tyr Ser Gly Trp Val Ala His Arg Ala Leu Ile Arg Gly Val Asn Leu 
            180                 185                 190         


Ala Gln His Ala Asp Val Phe Glu Pro Cys Val Lys Trp Trp Ser Glu 
        195                 200                 205             


Asp Arg His Met Met Val Tyr Tyr Thr Thr Gly Lys Arg Asp Glu Tyr 
    210                 215                 220                 


Tyr Phe Val Thr Gly Val Pro His Glu Ala Trp Asp Phe Gln Gly Ala 
225                 230                 235                 240 


Phe Val Asp Ser Ser Gln Glu Glu Met Arg Ala Ala Phe Glu Gly Tyr 
                245                 250                 255     


His Pro Thr Val Gln Lys Leu Ile Asp Ala Thr Glu Ser Ile Thr Lys 
            260                 265                 270         


Trp Pro Leu Arg Asn Arg Asn Pro Leu Pro Leu Trp Ser Arg Gly Arg 
        275                 280                 285             


Leu Val Leu Leu Gly Asp Ala Cys His Pro Met Lys Pro His Met Ala 
    290                 295                 300                 


Gln Gly Ala Cys Met Ala Ile Glu Asp Ala Ala Met Leu Thr Arg Cys 
305                 310                 315                 320 


Leu Gln Glu Thr Gly Leu Ser Asp His Arg Thr Ala Phe Ala Leu Tyr 
                325                 330                 335     


Glu Ala Asn Arg Lys Glu Arg Ala Ser Gln Val Gln Ser Val Ser Asn 
            340                 345                 350         


Ala Asn Thr Trp Leu Tyr Ser Gln Glu Asp Pro Ala Trp Val Tyr Gly 
        355                 360                 365             


Tyr Asp Leu Tyr Gly Gln Gln Leu Glu Ser Gly Glu Ala Ala 
    370                 375                 380         


<210>  20
<211>  350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2,5-dihydroxypyridine 5,6-dioxygenase, NicX (NCBI accession 
       NP_746075.1)

<400>  20

Met Pro Val Ser Asn Ala Gln Leu Thr Gln Met Phe Glu His Val Leu 
1               5                   10                  15      


Lys Leu Ser Arg Val Asp Glu Thr Gln Ser Val Ala Val Leu Lys Ser 
            20                  25                  30          


His Tyr Ser Asp Pro Arg Thr Val Asn Ala Ala Met Glu Ala Ala Gln 
        35                  40                  45              


Arg Leu Lys Ala Lys Val Tyr Ala Val Glu Leu Pro Ala Phe Asn His 
    50                  55                  60                  


Pro Thr Ala Met Gly Asn Asp Met Thr Ala Tyr Cys Gly Asp Thr Ala 
65                  70                  75                  80  


Leu Thr Gly Asn Leu Ala Ala Gln Arg Ala Leu Glu Ala Ala Asp Leu 
                85                  90                  95      


Val Val Asp Thr Met Met Leu Leu His Ser Pro Glu Gln Glu Gln Ile 
            100                 105                 110         


Leu Lys Thr Gly Thr Arg Ile Leu Leu Ala Val Glu Pro Pro Glu Val 
        115                 120                 125             


Leu Ala Arg Met Leu Pro Thr Glu Asp Asp Lys Arg Arg Val Leu Ala 
    130                 135                 140                 


Ala Glu Thr Leu Leu Lys Gln Ala Arg Ser Leu His Val Arg Ser Lys 
145                 150                 155                 160 


Ala Gly Ser Asp Phe His Ala Pro Leu Gly Gln Tyr Pro Ala Val Thr 
                165                 170                 175     


Glu Tyr Gly Tyr Ala Asp Glu Pro Gly Arg Trp Asp His Trp Pro Ser 
            180                 185                 190         


Gly Phe Leu Phe Thr Trp Pro Asn Glu Asp Ser Ala Glu Gly Thr Leu 
        195                 200                 205             


Val Leu Asp Val Gly Asp Ile Ile Leu Pro Phe Lys Asn Tyr Cys Arg 
    210                 215                 220                 


Glu Arg Ile Thr Leu Glu Ile Glu Lys Gly Phe Ile Thr Gly Ile His 
225                 230                 235                 240 


Gly Gly Phe Glu Ala Glu Tyr Leu Arg Asp Tyr Met Lys Tyr Phe Asn 
                245                 250                 255     


Asp Pro Glu Val Tyr Gly Ile Ser His Ile Gly Trp Gly Leu Gln Pro 
            260                 265                 270         


Arg Ala Gln Trp Thr Ala Met Gly Leu His Asp Arg Asn Asp Gly Met 
        275                 280                 285             


Cys Met Asp Ala Arg Ala Phe Tyr Gly Asn Phe Leu Phe Ser Thr Gly 
    290                 295                 300                 


Pro Asn Thr Glu Val Gly Gly Lys Arg Lys Thr Pro Cys His Leu Asp 
305                 310                 315                 320 


Ile Pro Leu Arg Asn Cys Asp Ile Tyr Leu Asp Asp Lys Ala Val Val 
                325                 330                 335     


Leu Ala Gly Asp Val Val Ala Pro Glu Glu Ser Arg Ala Arg 
            340                 345                 350 


<210>  21
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HTH-type transcriptional repressor, NicR (NCBI accession 
       NP_746076.1)

<400>  21

Met Ser Lys Lys Thr Thr Pro Ser Ser Ala Pro Leu Asp Asn Thr Pro 
1               5                   10                  15      


Tyr Asp Val Thr Glu Gln Val Gly His Leu Leu Arg Lys Ala Tyr Gln 
            20                  25                  30          


Arg His Thr Ala Ile Phe Gln Gln Gln Ala Cys Asp Pro Gln Leu Thr 
        35                  40                  45              


Ser Ile Gln Phe Val Thr Leu Cys Ala Leu Arg Asp His Gly Pro Ser 
    50                  55                  60                  


Ser Gln Ala Glu Leu Ile Lys Ala Thr Ala Val Asp Gln Ala Thr Ile 
65                  70                  75                  80  


Arg Gly Ile Val Glu Arg Leu Lys Ala Arg Glu Leu Val Gln Leu Ser 
                85                  90                  95      


Pro Asp Pro Gly Asp Arg Arg Lys Val Ile Val Glu Leu Thr Glu Ser 
            100                 105                 110         


Gly Ala Ala Leu Leu Asp Ala Met Ile Pro Cys Ala Arg Gln Ile Ser 
        115                 120                 125             


Glu Leu Ser Met Gly Ser Leu Asn Ala Gly Glu Arg Val Ala Ile Leu 
    130                 135                 140                 


Tyr Leu Leu Arg Lys Met Ile Asp Ser Asp Glu Asn Ala Gly 
145                 150                 155             


<210>  22
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nicotinate dehydrogenase subunit A, NicA (NCBI accession 
       NP_746077.1)

<400>  22

Met Gln Thr Thr Ile Ser Leu Gln Val Asn Gly Gln Pro Val Glu Val 
1               5                   10                  15      


Ser Ala Met Pro Asp Thr Pro Leu Leu Leu Ile Leu Arg Asn Asp Leu 
            20                  25                  30          


Cys Leu Asn Gly Pro Lys Tyr Gly Cys Gly Leu Gly Glu Cys Gly Ala 
        35                  40                  45              


Cys Thr Val Ile Ile Asp Gly Val Ala Ala Arg Ser Cys Val Ile Pro 
    50                  55                  60                  


Leu Ala Gly Ala Ala Gly Arg Asn Ile Thr Thr Leu Glu Gly Leu Gly 
65                  70                  75                  80  


Ser Lys Ala Ala Pro His Pro Val Gln Gln Ala Phe Ile Asp Glu Gln 
                85                  90                  95      


Ala Ala Gln Cys Gly Tyr Cys Met Asn Gly Met Ile Met Thr Ala Lys 
            100                 105                 110         


Ala Leu Leu Asp Arg Ile Pro Glu Pro Ser Asp Glu Gln Ile Arg Asn 
        115                 120                 125             


Glu Leu Ser Ala Asn Leu Cys Arg Cys Gly Thr His Val Glu Ile Leu 
    130                 135                 140                 


Arg Ala Val Arg Arg Ala Ala Glu Thr Arg Arg Lys Pro 
145                 150                 155         


<210>  23
<211>  1187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nicotinate dehydrogenase subunit B, NicB (NCBI accession 
       NP_746078.1)

<400>  23

Met Asn His Ser Gln Gln Val Pro Ser Arg Asp Gln Leu Leu Ala Lys 
1               5                   10                  15      


Thr Gly Val Leu Leu Ile Val Asp Gln Ile Thr Pro Pro Ser Gly Pro 
            20                  25                  30          


Val Ala Lys Gly Val Thr Pro Thr Val Lys Glu Arg Glu Leu Ala Leu 
        35                  40                  45              


Phe Ile Ala Val Ser Asp Asp Gly Met Val Tyr Ala Phe Asn Gly His 
    50                  55                  60                  


Val Asp Leu Gly Thr Gly Ile Arg Thr Ser Leu Ala Gln Ile Val Ala 
65                  70                  75                  80  


Glu Glu Leu Asp Leu Arg Met Asp Gln Val His Met Val Leu Gly Asp 
                85                  90                  95      


Thr Glu Arg Ala Pro Asn Gln Gly Ala Thr Ile Ala Ser Ala Thr Leu 
            100                 105                 110         


Gln Ile Ser Ala Val Pro Leu Arg Lys Ala Ala Ala Thr Ala Arg Arg 
        115                 120                 125             


Tyr Leu Leu Gln Gln Ala Ala Leu Arg Leu Gly Cys Pro Pro Glu Met 
    130                 135                 140                 


Leu Arg Ile Glu Asp Gly Thr Val Ile Ala Ser Asn Gly Ser Thr Leu 
145                 150                 155                 160 


Ser Phe Ala Glu Leu Val Gln Gly Lys Asn His Gln Leu His Ile Ala 
                165                 170                 175     


Asp Asp Ala Pro Leu Lys Ala Ile Glu Asp Tyr Arg Leu Val Gly Arg 
            180                 185                 190         


Ser Ala Pro Arg Val Asp Ile Pro Gly Lys Ala Thr Gly Glu Leu Thr 
        195                 200                 205             


Tyr Val His Asp Met Arg Leu Pro Asn Met Leu His Gly Arg Val Ile 
    210                 215                 220                 


Arg Pro Pro Tyr Ala Gly His Asp Ser Gly Asp Phe Val Gly Asn Ser 
225                 230                 235                 240 


Leu Leu Ala Val Asp Glu Ser Ser Ile Ala His Leu Pro Gly Val Val 
                245                 250                 255     


Ala Val Val Val Ile Arg Asp Phe Val Gly Val Val Ala Glu Arg Glu 
            260                 265                 270         


Glu Gln Ala Ile Arg Ala Ala His Glu Leu Lys Val Ser Trp Lys Pro 
        275                 280                 285             


Phe Thr Gly Lys Leu Pro Asp Leu Ser Asp Val Ala Gln Ala Ile Arg 
    290                 295                 300                 


Asp Asn Pro Arg Val Gln Arg Thr Val Leu Asp Gln Gly Asp Val Asp 
305                 310                 315                 320 


Gly Gly Ile Ala Asn Ala Ser Gln Arg Leu Ser Arg Ser Tyr Leu Trp 
                325                 330                 335     


Pro Tyr Gln Leu His Ala Ser Ile Gly Pro Ser Cys Ala Leu Ala Asp 
            340                 345                 350         


Phe Thr Ala Gly Gln Ile Arg Val Trp Ser Gly Thr Gln Asn Pro His 
        355                 360                 365             


Leu Leu Arg Ala Asp Leu Ala Trp Leu Leu Ala Cys Asp Glu Ala Arg 
    370                 375                 380                 


Ile Glu Ile Ile Arg Met Glu Ala Ala Gly Cys Tyr Gly Arg Asn Cys 
385                 390                 395                 400 


Ala Asp Asp Val Cys Ala Asp Ala Val Leu Leu Ser Arg Ala Val Gln 
                405                 410                 415     


Arg Pro Val Arg Val Gln Leu Thr Arg Glu Gln Glu His Val Trp Glu 
            420                 425                 430         


Pro Lys Gly Thr Ala Gln Leu Met Glu Ile Asp Gly Gly Leu Asn Ala 
        435                 440                 445             


Asp Gly Ser Val Ala Ala Tyr Asp Phe Gln Thr Ser Tyr Pro Ser Asn 
    450                 455                 460                 


Gly Ala Pro Thr Leu Ala Leu Leu Leu Thr Gly Ala Val Glu Pro Val 
465                 470                 475                 480 


Pro Ala Leu Phe Glu Met Gly Asp Arg Thr Ser Ile Pro Pro Tyr Asp 
                485                 490                 495     


Tyr Glu His Met Arg Val Thr Ile Asn Asp Met Thr Pro Leu Val Arg 
            500                 505                 510         


Ala Ser Trp Met Arg Gly Val Ser Ala Met Pro Asn Ser Phe Ala His 
        515                 520                 525             


Glu Ser Tyr Ile Asp Glu Leu Ala Phe Ala Ala Gly Val Asp Pro Val 
    530                 535                 540                 


Glu Tyr Arg Leu Lys His Leu Ser Asp Pro Arg Ala Ile Asp Leu Val 
545                 550                 555                 560 


Lys Ala Thr Ala Glu Arg Ala Gln Trp Gln Pro His Thr Arg Pro Met 
                565                 570                 575     


Gln Thr Gln Ala Glu Gly Asp Val Leu Arg Gly Arg Gly Phe Ala Tyr 
            580                 585                 590         


Ala Arg Tyr Ile His Ser Lys Phe Pro Gly Phe Gly Ala Ala Trp Ala 
        595                 600                 605             


Ala Trp Val Ala Asp Val Ala Val Asp Arg Arg Thr Gly Glu Val Ala 
    610                 615                 620                 


Val Thr Arg Val Val Ile Gly His Asp Ala Gly Met Met Val Asn Pro 
625                 630                 635                 640 


Glu Gly Val Arg His Gln Ile His Gly Asn Val Ile Gln Ser Thr Ser 
                645                 650                 655     


Arg Val Leu Lys Glu Gln Val Ser Phe Glu Glu Ser Thr Val Ala Ser 
            660                 665                 670         


Lys Glu Trp Gly Gly Tyr Pro Ile Leu Thr Phe Pro Glu Leu Pro Ala 
        675                 680                 685             


Ile Asp Val Met Met Leu Pro Arg Gln His Glu Pro Pro Met Gly Ser 
    690                 695                 700                 


Gly Glu Ser Ala Ser Val Pro Ser Ala Ala Ala Ile Ala Asn Ala Ile 
705                 710                 715                 720 


Phe Asp Ala Thr Gly Ile Arg Phe Arg Glu Leu Pro Ile Thr Ala Glu 
                725                 730                 735     


Arg Val Arg Ala Ala Leu Gly Gly Glu Gly Gln Gly Pro Asp Ala Pro 
            740                 745                 750         


Ala Pro Ala Gln Pro Ser Thr Lys Arg Ser Lys Trp Trp Phe Gly Ser 
        755                 760                 765             


Leu Ala Gly Val Phe Gly Ala Ala Leu Gly Met Leu Ala Thr Ala Leu 
    770                 775                 780                 


Pro Trp Arg Ala Glu Ile Ala Pro Val Thr Pro Pro Gly Val Gly Ser 
785                 790                 795                 800 


Trp Ser Ala Ala Met Leu Glu Arg Gly Arg Gln Val Ala Ala Ala Gly 
                805                 810                 815     


Asp Cys Ala Val Cys His Thr Val Ser Gly Gly Lys Ala Asn Ala Gly 
            820                 825                 830         


Gly Leu Ala Met Asp Thr Pro Phe Gly Thr Leu Tyr Ser Thr Asn Ile 
        835                 840                 845             


Thr Pro Asp Pro Glu Thr Gly Ile Gly Arg Trp Ser Phe Ala Ala Phe 
    850                 855                 860                 


Glu Arg Ala Met Arg Glu Gly Ile Ser Arg Asp Gly Arg His Leu Tyr 
865                 870                 875                 880 


Pro Ala Phe Pro Tyr Thr Ser Phe Arg Asn Ile Asn Asp Ala Asp Met 
                885                 890                 895     


Gln Ala Leu Tyr Ala Tyr Leu Met Ser Gln Thr Pro Val Arg Gln Glu 
            900                 905                 910         


Ala Pro Ala Asn Gln Met Arg Phe Pro Phe Asn Gln Arg Pro Leu Met 
        915                 920                 925             


Ala Gly Trp Asn Ala Arg Phe Leu Gln Arg Gly Glu Tyr Gln Pro Asp 
    930                 935                 940                 


Pro Gln Arg Ser Ala Gln Trp Asn Arg Gly Ala Tyr Leu Val Asp Gly 
945                 950                 955                 960 


Leu Gly His Cys Thr Ala Cys His Ser Pro Arg Asn Leu Met Gly Ala 
                965                 970                 975     


Glu Lys Gly Gly Ser Ser Tyr Leu Ala Gly Gly Met Val Asp Gly Trp 
            980                 985                 990         


Glu Ala Pro Ala Leu Asn Ala Leu  Gly Lys Ser Ser Thr  Pro Trp Ser 
        995                 1000                 1005             


Glu Asp  Glu Leu Phe Asn Tyr  Leu Ser Thr Gly Phe  Ser Glu Lys 
    1010                 1015                 1020             


His Gly  Val Ala Ala Gly Pro  Met Gly Pro Val Val  Ser Glu Leu 
    1025                 1030                 1035             


Ala Thr  Leu Pro Lys Ser Asp  Val Arg Ala Ile Ala  His Tyr Leu 
    1040                 1045                 1050             


Ser Ser  Leu Glu Gly Glu Pro  Gln Ala Leu Ala Ala  Asn Ala Ala 
    1055                 1060                 1065             


Pro Gln  Val Asp Thr His Val  Ser Leu Ser Asn Gly  Glu Arg Val 
    1070                 1075                 1080             


Phe Lys  Gly Ala Cys Leu Gly  Cys His Ser Asp Gly  Leu Gly Pro 
    1085                 1090                 1095             


Lys Leu  Phe Gly Val Ser Pro  Ser Met Ala Val Asn  Ser Asn Val 
    1100                 1105                 1110             


His Ser  Asp Leu Pro Asp Asn  Leu Leu Arg Val Val  Leu His Gly 
    1115                 1120                 1125             


Ile Pro  Thr Pro Ala Thr Arg  Asp Leu Gly Tyr Met  Pro Gly Phe 
    1130                 1135                 1140             


Lys Asp  Ser Leu Ser Asp Arg  Gln Val Ala Asp Leu  Ala Ala Tyr 
    1145                 1150                 1155             


Leu Arg  His Arg Phe Ala Ala  Asp Lys Pro Ala Trp  Gln Gly Leu 
    1160                 1165                 1170             


Ala Ser  Lys Ala Ala Gln Val  Arg Ala Asn Pro Gly  Ser His 
    1175                 1180                 1185         


<210>  24
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HTH-type transcriptional repressor, NicS (NCBI accession NP_ 
       NP_746079.1)

<400>  24

Met Gln Thr Arg Lys Thr Gly Val Arg Ala Gln Gln Ala Asp Arg Thr 
1               5                   10                  15      


Arg Asp Asn Ile Leu Lys Ala Ala Val Lys Val Phe Ser Lys Glu Gly 
            20                  25                  30          


Phe Thr Gly Gly Arg Ile Glu Gln Ile Ser Thr Leu Ala Lys Ser Asn 
        35                  40                  45              


Asp Arg Met Ile Tyr Tyr Tyr Phe Gly Ser Lys Glu Lys Leu Phe Ile 
    50                  55                  60                  


Ser Val Leu Glu His Ile Tyr Ala Ser Phe Asn Gln Ala Glu Ala Lys 
65                  70                  75                  80  


Leu Arg Leu Asp Leu Ala Asp Pro Glu Gln Ala Leu Arg Glu Leu Val 
                85                  90                  95      


Ala Phe Ile Trp Asp Tyr Tyr Val Arg His Pro Glu Phe Val Thr Ile 
            100                 105                 110         


Leu Ala Thr Glu Asn Leu His Gln Gly Leu His Ala Arg Lys Ser Gln 
        115                 120                 125             


Asn Leu Arg Ala Leu Ser Gly Glu Ala Val Gly Val Leu Arg Pro Ile 
    130                 135                 140                 


Ile Glu Ala Gly Gln Ala Lys Gly Leu Phe Arg Asp Asp Ile Cys Ile 
145                 150                 155                 160 


Thr His Ala Tyr Leu Met Ile Ala Ser Leu Cys Tyr Phe Tyr Asn Ser 
                165                 170                 175     


Asn Arg His Thr Leu Ser Ser Phe Leu Ala Val Asp Leu Ala Asp Lys 
            180                 185                 190         


Gln Ala Lys Ala Asp Trp Leu Thr Phe Ile Ser Asp Leu Ala Leu Arg 
        195                 200                 205             


Gly Leu Arg Arg 
    210         


