                         SEQUENCE LISTING

<110>  YALE UNIVERSITY
       Bindra, Ranjit
       Fons, Nathan
       Brenner, Charles M.
 
<120>  Identification of PPM1D mutations as a novel biomarker for NAMPTi
        sensitivity

<130>  047162-7195WO1(00970)

<150>  US 62/748,911
<151>  2018-10-22

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA oligo

<400>  1
acaccgttga gggtatgact acacctg                                           27


<210>  2
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA oligo

<400>  2
aaaacaggtg tagtcatacc ctcaacg                                           27


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  3
gcatagattt gttgagttct ggg                                               23


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  4
agccctctta tatcctaagt ttgg                                              24


<210>  5
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  5
ccagtcaagt cactcgagga ggatccatga ccaagggtga attc                        44


<210>  6
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
gaattcaccc ttggtcatgg atcctcctcg agtgacttga ctgg                        44


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
cacctctggt gaccaagacc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  8
gtggcctggt agaggtcagt                                                   20


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
cgagaggagt tgggtgacat cc                                                22


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
cctatggcgc actccctgtg                                                   20


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  11
tgactacttt tgacttcagc c                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
tctgcatttt aactatggct c                                                 21


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
aaacaggatg cctgccttta                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
ggactttcca cctatgggac                                                   20


<210>  15
<211>  491
<212>  PRT
<213>  Homo sapiens

<400>  15

Met Asn Pro Ala Ala Glu Ala Glu Phe Asn Ile Leu Leu Ala Thr Asp 
1               5                   10                  15      


Ser Tyr Lys Val Thr His Tyr Lys Gln Tyr Pro Pro Asn Thr Ser Lys 
            20                  25                  30          


Val Tyr Ser Tyr Phe Glu Cys Arg Glu Lys Lys Thr Glu Asn Ser Lys 
        35                  40                  45              


Leu Arg Lys Val Lys Tyr Glu Glu Thr Val Phe Tyr Gly Leu Gln Tyr 
    50                  55                  60                  


Ile Leu Asn Lys Tyr Leu Lys Gly Lys Val Val Thr Lys Glu Lys Ile 
65                  70                  75                  80  


Gln Glu Ala Lys Asp Val Tyr Lys Glu His Phe Gln Asp Asp Val Phe 
                85                  90                  95      


Asn Glu Lys Gly Trp Asn Tyr Ile Leu Glu Lys Tyr Asp Gly His Leu 
            100                 105                 110         


Pro Ile Glu Ile Lys Ala Val Pro Glu Gly Phe Val Ile Pro Arg Gly 
        115                 120                 125             


Asn Val Leu Phe Thr Val Glu Asn Thr Asp Pro Glu Cys Tyr Trp Leu 
    130                 135                 140                 


Thr Asn Trp Ile Glu Thr Ile Leu Val Gln Ser Trp Tyr Pro Ile Thr 
145                 150                 155                 160 


Val Ala Thr Asn Ser Arg Glu Gln Lys Lys Ile Leu Ala Lys Tyr Leu 
                165                 170                 175     


Leu Glu Thr Ser Gly Asn Leu Asp Gly Leu Glu Tyr Lys Leu His Asp 
            180                 185                 190         


Phe Gly Tyr Arg Gly Val Ser Ser Gln Glu Thr Ala Gly Ile Gly Ala 
        195                 200                 205             


Ser Ala His Leu Val Asn Phe Lys Gly Thr Asp Thr Val Ala Gly Leu 
    210                 215                 220                 


Ala Leu Ile Lys Lys Tyr Tyr Gly Thr Lys Asp Pro Val Pro Gly Tyr 
225                 230                 235                 240 


Ser Val Pro Ala Ala Glu His Ser Thr Ile Thr Ala Trp Gly Lys Asp 
                245                 250                 255     


His Glu Lys Asp Ala Phe Glu His Ile Val Thr Gln Phe Ser Ser Val 
            260                 265                 270         


Pro Val Ser Val Val Ser Asp Ser Tyr Asp Ile Tyr Asn Ala Cys Glu 
        275                 280                 285             


Lys Ile Trp Gly Glu Asp Leu Arg His Leu Ile Val Ser Arg Ser Thr 
    290                 295                 300                 


Gln Ala Pro Leu Ile Ile Arg Pro Asp Ser Gly Asn Pro Leu Asp Thr 
305                 310                 315                 320 


Val Leu Lys Val Leu Glu Ile Leu Gly Lys Lys Phe Pro Val Thr Glu 
                325                 330                 335     


Asn Ser Lys Gly Tyr Lys Leu Leu Pro Pro Tyr Leu Arg Val Ile Gln 
            340                 345                 350         


Gly Asp Gly Val Asp Ile Asn Thr Leu Gln Glu Ile Val Glu Gly Met 
        355                 360                 365             


Lys Gln Lys Met Trp Ser Ile Glu Asn Ile Ala Phe Gly Ser Gly Gly 
    370                 375                 380                 


Gly Leu Leu Gln Lys Leu Thr Arg Asp Leu Leu Asn Cys Ser Phe Lys 
385                 390                 395                 400 


Cys Ser Tyr Val Val Thr Asn Gly Leu Gly Ile Asn Val Phe Lys Asp 
                405                 410                 415     


Pro Val Ala Asp Pro Asn Lys Arg Ser Lys Lys Gly Arg Leu Ser Leu 
            420                 425                 430         


His Arg Thr Pro Ala Gly Asn Phe Val Thr Leu Glu Glu Gly Lys Gly 
        435                 440                 445             


Asp Leu Glu Glu Tyr Gly Gln Asp Leu Leu His Thr Val Phe Lys Asn 
    450                 455                 460                 


Gly Lys Val Thr Lys Ser Tyr Ser Phe Asp Glu Ile Arg Lys Asn Ala 
465                 470                 475                 480 


Gln Leu Asn Ile Glu Leu Glu Ala Ala His His 
                485                 490     


<210>  16
<211>  605
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Ala Gly Leu Tyr Ser Leu Gly Val Ser Val Phe Ser Asp Gln Gly 
1               5                   10                  15      


Gly Arg Lys Tyr Met Glu Asp Val Thr Gln Ile Val Val Glu Pro Glu 
            20                  25                  30          


Pro Thr Ala Glu Glu Lys Pro Ser Pro Arg Arg Ser Leu Ser Gln Pro 
        35                  40                  45              


Leu Pro Pro Arg Pro Ser Pro Ala Ala Leu Pro Gly Gly Glu Val Ser 
    50                  55                  60                  


Gly Lys Gly Pro Ala Val Ala Ala Arg Glu Ala Arg Asp Pro Leu Pro 
65                  70                  75                  80  


Asp Ala Gly Ala Ser Pro Ala Pro Ser Arg Cys Cys Arg Arg Arg Ser 
                85                  90                  95      


Ser Val Ala Phe Phe Ala Val Cys Asp Gly His Gly Gly Arg Glu Ala 
            100                 105                 110         


Ala Gln Phe Ala Arg Glu His Leu Trp Gly Phe Ile Lys Lys Gln Lys 
        115                 120                 125             


Gly Phe Thr Ser Ser Glu Pro Ala Lys Val Cys Ala Ala Ile Arg Lys 
    130                 135                 140                 


Gly Phe Leu Ala Cys His Leu Ala Met Trp Lys Lys Leu Ala Glu Trp 
145                 150                 155                 160 


Pro Lys Thr Met Thr Gly Leu Pro Ser Thr Ser Gly Thr Thr Ala Ser 
                165                 170                 175     


Val Val Ile Ile Arg Gly Met Lys Met Tyr Val Ala His Val Gly Asp 
            180                 185                 190         


Ser Gly Val Val Leu Gly Ile Gln Asp Asp Pro Lys Asp Asp Phe Val 
        195                 200                 205             


Arg Ala Val Glu Val Thr Gln Asp His Lys Pro Glu Leu Pro Lys Glu 
    210                 215                 220                 


Arg Glu Arg Ile Glu Gly Leu Gly Gly Ser Val Met Asn Lys Ser Gly 
225                 230                 235                 240 


Val Asn Arg Val Val Trp Lys Arg Pro Arg Leu Thr His Asn Gly Pro 
                245                 250                 255     


Val Arg Arg Ser Thr Val Ile Asp Gln Ile Pro Phe Leu Ala Val Ala 
            260                 265                 270         


Arg Ala Leu Gly Asp Leu Trp Ser Tyr Asp Phe Phe Ser Gly Glu Phe 
        275                 280                 285             


Val Val Ser Pro Glu Pro Asp Thr Ser Val His Thr Leu Asp Pro Gln 
    290                 295                 300                 


Lys His Lys Tyr Ile Ile Leu Gly Ser Asp Gly Leu Trp Asn Met Ile 
305                 310                 315                 320 


Pro Pro Gln Asp Ala Ile Ser Met Cys Gln Asp Gln Glu Glu Lys Lys 
                325                 330                 335     


Tyr Leu Met Gly Glu His Gly Gln Ser Cys Ala Lys Met Leu Val Asn 
            340                 345                 350         


Arg Ala Leu Gly Arg Trp Arg Gln Arg Met Leu Arg Ala Asp Asn Thr 
        355                 360                 365             


Ser Ala Ile Val Ile Cys Ile Ser Pro Glu Val Asp Asn Gln Gly Asn 
    370                 375                 380                 


Phe Thr Asn Glu Asp Glu Leu Tyr Leu Asn Leu Thr Asp Ser Pro Ser 
385                 390                 395                 400 


Tyr Asn Ser Gln Glu Thr Cys Val Met Thr Pro Ser Pro Cys Ser Thr 
                405                 410                 415     


Pro Pro Val Lys Ser Leu Glu Glu Asp Pro Trp Pro Arg Val Asn Ser 
            420                 425                 430         


Lys Asp His Ile Pro Ala Leu Val Arg Ser Asn Ala Phe Ser Glu Asn 
        435                 440                 445             


Phe Leu Glu Val Ser Ala Glu Ile Ala Arg Glu Asn Val Gln Gly Val 
    450                 455                 460                 


Val Ile Pro Ser Lys Asp Pro Glu Pro Leu Glu Glu Asn Cys Ala Lys 
465                 470                 475                 480 


Ala Leu Thr Leu Arg Ile His Asp Ser Leu Asn Asn Ser Leu Pro Ile 
                485                 490                 495     


Gly Leu Val Pro Thr Asn Ser Thr Asn Thr Val Met Asp Gln Lys Asn 
            500                 505                 510         


Leu Lys Met Ser Thr Pro Gly Gln Met Lys Ala Gln Glu Ile Glu Arg 
        515                 520                 525             


Thr Pro Pro Thr Asn Phe Lys Arg Thr Leu Glu Glu Ser Asn Ser Gly 
    530                 535                 540                 


Pro Leu Met Lys Lys His Arg Arg Asn Gly Leu Ser Arg Ser Ser Gly 
545                 550                 555                 560 


Ala Gln Pro Ala Ser Leu Pro Thr Thr Ser Gln Arg Lys Asn Ser Val 
                565                 570                 575     


Lys Leu Thr Met Arg Arg Arg Leu Arg Gly Gln Lys Lys Ile Gly Asn 
            580                 585                 590         


Pro Leu Leu His Gln His Arg Lys Thr Val Cys Val Cys 
        595                 600                 605 


