                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  GENE THERAPY FOR RETINAL DISEASE

<130>  N413815WO

<150>  GB 1817469.8
<151>  2018-10-26

<160>  65    

<170>  PatentIn version 3.5

<210>  1
<211>  1047
<212>  DNA
<213>  Homo sapiens

<400>  1
atgaatggca cagaaggccc taacttctac gtgcccttct ccaatgcgac gggtgtggta       60

cgcagcccct tcgagtaccc acagtactac ctggctgagc catggcagtt ctccatgctg      120

gccgcctaca tgtttctgct gatcgtgctg ggcttcccca tcaacttcct cacgctctac      180

gtcaccgtcc agcacaagaa gctgcgcacg cctctcaact acatcctgct caacctagcc      240

gtggctgacc tcttcatggt cctaggtggc ttcaccagca ccctctacac ctctctgcat      300

ggatacttcg tcttcgggcc cacaggatgc aatttggagg gcttctttgc caccctgggc      360

ggtgaaattg ccctgtggtc cttggtggtc ctggccatcg agcggtacgt ggtggtgtgt      420

aagcccatga gcaacttccg cttcggggag aaccatgcca tcatgggcgt tgccttcacc      480

tgggtcatgg cgctggcctg cgccgcaccc ccactcgccg gctggtccag gtacatcccc      540

gagggcctgc agtgctcgtg tggaatcgac tactacacgc tcaagccgga ggtcaacaac      600

gagtcttttg tcatctacat gttcgtggtc cacttcacca tccccatgat tatcatcttt      660

ttctgctatg ggcagctcgt cttcaccgtc aaggaggccg ctgcccagca gcaggagtca      720

gccaccacac agaaggcaga gaaggaggtc acccgcatgg tcatcatcat ggtcatcgct      780

ttcctgatct gctgggtgcc ctacgccagc gtggcattct acatcttcac ccaccagggc      840

tccaacttcg gtcccatctt catgaccatc ccagcgttct ttgccaagag cgccgccatc      900

tacaaccctg tcatctatat catgatgaac aagcagttcc ggaactgcat gctcaccacc      960

atctgctgcg gcaagaaccc actgggtgac gatgaggcct ctgctaccgt gtccaagacg     1020

gagacgagcc aggtggcccc ggcctaa                                         1047


<210>  2
<211>  348
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Ala 
1               5                   10                  15      


Thr Gly Val Val Arg Ser Pro Phe Glu Tyr Pro Gln Tyr Tyr Leu Ala 
            20                  25                  30          


Glu Pro Trp Gln Phe Ser Met Leu Ala Ala Tyr Met Phe Leu Leu Ile 
        35                  40                  45              


Val Leu Gly Phe Pro Ile Asn Phe Leu Thr Leu Tyr Val Thr Val Gln 
    50                  55                  60                  


His Lys Lys Leu Arg Thr Pro Leu Asn Tyr Ile Leu Leu Asn Leu Ala 
65                  70                  75                  80  


Val Ala Asp Leu Phe Met Val Leu Gly Gly Phe Thr Ser Thr Leu Tyr 
                85                  90                  95      


Thr Ser Leu His Gly Tyr Phe Val Phe Gly Pro Thr Gly Cys Asn Leu 
            100                 105                 110         


Glu Gly Phe Phe Ala Thr Leu Gly Gly Glu Ile Ala Leu Trp Ser Leu 
        115                 120                 125             


Val Val Leu Ala Ile Glu Arg Tyr Val Val Val Cys Lys Pro Met Ser 
    130                 135                 140                 


Asn Phe Arg Phe Gly Glu Asn His Ala Ile Met Gly Val Ala Phe Thr 
145                 150                 155                 160 


Trp Val Met Ala Leu Ala Cys Ala Ala Pro Pro Leu Ala Gly Trp Ser 
                165                 170                 175     


Arg Tyr Ile Pro Glu Gly Leu Gln Cys Ser Cys Gly Ile Asp Tyr Tyr 
            180                 185                 190         


Thr Leu Lys Pro Glu Val Asn Asn Glu Ser Phe Val Ile Tyr Met Phe 
        195                 200                 205             


Val Val His Phe Thr Ile Pro Met Ile Ile Ile Phe Phe Cys Tyr Gly 
    210                 215                 220                 


Gln Leu Val Phe Thr Val Lys Glu Ala Ala Ala Gln Gln Gln Glu Ser 
225                 230                 235                 240 


Ala Thr Thr Gln Lys Ala Glu Lys Glu Val Thr Arg Met Val Ile Ile 
                245                 250                 255     


Met Val Ile Ala Phe Leu Ile Cys Trp Val Pro Tyr Ala Ser Val Ala 
            260                 265                 270         


Phe Tyr Ile Phe Thr His Gln Gly Ser Asn Phe Gly Pro Ile Phe Met 
        275                 280                 285             


Thr Ile Pro Ala Phe Phe Ala Lys Ser Ala Ala Ile Tyr Asn Pro Val 
    290                 295                 300                 


Ile Tyr Ile Met Met Asn Lys Gln Phe Arg Asn Cys Met Leu Thr Thr 
305                 310                 315                 320 


Ile Cys Cys Gly Lys Asn Pro Leu Gly Asp Asp Glu Ala Ser Ala Thr 
                325                 330                 335     


Val Ser Lys Thr Glu Thr Ser Gln Val Ala Pro Ala 
            340                 345             


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 1

<400>  3
atctacatgt tcgtggtcca c                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 2

<400>  4
atcaacttcc tcacgctcta c                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 3

<400>  5
cttccccatc aacttcctca c                                                 21


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 4

<400>  6
cttcctcacg ctctacgtca c                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 5H

<400>  7
aacgagtctt ttgtcatcta c                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 6

<400>  8
catgttcgtg gtccacttca c                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target sequence of mirtron 7

<400>  9
gttccggaac tgcatgctca c                                                 21


<210>  10
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 1

<400>  10
gtggaccacg aacatgtaga tttcaagaga atctacatgt tcgtggtccc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  11
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 2

<400>  11
gtagagcgtg aggaagttga tttcaagaga atcaacttcc tcacgctttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  12
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 3

<400>  12
gtgaggaagt tgatggggaa gttcaagaga cttccccatc aacttctttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  13
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 4

<400>  13
gtgacgtaga gcgtgaggaa gttcaagaga cttcctcacg ctctacgttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  14
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 5H

<400>  14
gtagatgaca aaagactcgt tttcaagaga aacgagtctt ttgtcatttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  15
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 6

<400>  15
gtgaagtgga ccacgaacat gttcaagaga catgttcgtg gtccactttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  16
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 7

<400>  16
gtgagcatgc agttccggaa cttcaagaga gttccggaac tgcatgtttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide strand sequence of mirtron 1

<400>  17
gtggaccacg aacatgtaga t                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide strand sequence of mirtron 2

<400>  18
gtagagcgtg aggaagttga t                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide strand sequence of mirtron 3

<400>  19
gtgaggaagt tgatggggaa g                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Guide strand sequence of mirtron 4

<400>  20
gtgacgtaga gcgtgaggaa g                                                 21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide strand sequence of mirtron 5

<400>  21
gtagatgaca aaagactcgt t                                                 21


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide strand sequence of mirtron 6

<400>  22
gtgaagtgga ccacgaacat g                                                 21


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Guide strand sequence of mirtron 7

<400>  23
gtgagcatgc agttccggaa c                                                 21


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified target sequence of mirtron 3

<400>  24
atttccaatt aattttctga c                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified target sequence of mirtron 5H

<400>  25
aatgaatcct tcgtgattta t                                                 21


<210>  26
<211>  340
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'UTR insert (eGFP - M3 - eGFP - M5 - eGFP)

<400>  26
tgacgggaac tacaagaccc gcgctgaagt caagttcgaa ggtgaggaag ttgatgggga       60

agttcaagag acttccccat caacttcttt ctcagttttt tctctttctt ttcgaaggtg      120

acaccctggt gaatagaatc gagctgaagg gcattgactt taaggaggat tgacgggaac      180

tacaagaccc gcgctgaagt caagttcgaa ggtagatgac aaaagactcg ttttcaagag      240

aaacgagtct tttgtcattt ctcagttttt tctctttctt ttcgaaggtg acaccctggt      300

gaatagaatc gagctgaagg gcattgactt taaggaggat                            340


<210>  27
<211>  779
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'UTR with mirtrons and additional ESE-rich sequences

<400>  27
ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct      120

ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa      180

agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gctgtccgcg      240

gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac      300

cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct      360

gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgg atcctagctt      420

gattgacggg aactacaaga cccgcgctga agtcaagttc gaaggtgagg aagttgatgg      480

ggaagttcaa gagacttccc catcaacttc tttctcagtt ttttctcttt cttttcgaag      540

gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag gattgacggg      600

aactacaaga cccgcgctga agtcaagttc gaaggtagat gacaaaagac tcgttttcaa      660

gagaaacgag tcttttgtca tttctcagtt ttttctcttt cttttcgaag gtgacaccct      720

ggtgaataga atcgagctga agggcattga ctttaaggag gatatcgaat tccgccacc       779


<210>  28
<211>  3947
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV sequence

<400>  28
cacatacgat ttaggtgaca ctatagaata cacggaatta attctagctg cgcgctcgct       60

cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct      120

cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt      180

agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg tacccagatc      240

ttccccacct agccacctgg caaactgctc cttctctcaa aggcccaaac atggcctccc      300

agactgcaac ccccaggcag tcaggccctg tctccacaac ctcacagcca ccctggacgg      360

aatctgcttc ttcccacatt tgagtcctcc tcagcccctg agctcctctg ggcagggctg      420

tttctttcca tctttgtatt cccaggggcc tgcaaataaa tgtttaatga acgaacaaga      480

gagtgaattc caattccatg caacaaggat tgggctcctg ggccctaggc tatgtgtctg      540

gcaccagaaa cggaagctgc aggttgcagc ccctgccctc atggagctcc tcctgtcaga      600

ggagtgtggg gactggatga ctccagaggt aacttgtggg ggaacgaaca ggtaaggggc      660

tgtgtgacga gatgagagac tgggagaata aaccagaaag tctctagctg tccagaggac      720

atagcacaga ggcccatggt ccctatttca aacccaggcc accagactga gctgggacct      780

tgggacagac aagtcatgca gaagttaggg gaccttctcc tcccttttcc tggatcctga      840

gtacctctcc tccctgacct caggcttcct cctagtgtca ccttggcccc tcttagaagc      900

caattaggcc ctcagtttct gcagcgggga ttaatatgat tatgaacacc cccaatctcc      960

cagatgctga ttcagccagg agcttaggag ggggaggtca ctttataagg gtctgggggg     1020

gtcagaaccc agagtcatcc ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg     1080

ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg     1140

ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt     1200

cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga     1260

gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg     1320

ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc     1380

ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc     1440

aaagaattgg atcctagctt gattgacggg aactacaaga cccgcgctga agtcaagttc     1500

gaaggtgagg aagttgatgg ggaagttcaa gagacttccc catcaacttc tttctcagtt     1560

ttttctcttt cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga     1620

ctttaaggag gattgacggg aactacaaga cccgcgctga agtcaagttc gaaggtagat     1680

gacaaaagac tcgttttcaa gagaaacgag tcttttgtca tttctcagtt ttttctcttt     1740

cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag     1800

gatatcgaat tccgccacca tgaatggcac agaaggccct aacttctacg tgcccttctc     1860

caatgcgacg ggtgtggtac gcagcccctt cgagtaccca cagtactacc tggctgagcc     1920

atggcagttc tccatgctgg ccgcctacat gtttctgctg atcgtgctgg gatttccaat     1980

taattttctg acgctctacg tcaccgtcca gcacaagaag ctgcgcacgc ctctcaacta     2040

catcctgctc aacctagccg tggctgacct cttcatggtc ctaggtggct tcaccagcac     2100

cctctacacc tctctgcatg gatacttcgt cttcgggccc acaggatgca atttggaggg     2160

cttctttgcc accctgggcg gtgaaattgc cctgtggtcc ttggtggtcc tggccatcga     2220

gcggtacgtg gtggtgtgta agcccatgag caacttccgc ttcggggaga accatgccat     2280

catgggcgtt gccttcacct gggtcatggc gctggcctgc gccgcacccc cactcgccgg     2340

ctggtccagg tacatccccg agggcctgca gtgctcgtgt ggaatcgact actacacgct     2400

caagccggag gtcaacaatg aatccttcgt gatttatatg ttcgtggtcc acttcaccat     2460

ccccatgatt atcatctttt tctgctatgg gcagctcgtc ttcaccgtca aggaggccgc     2520

tgcccagcag caggagtcag ccaccacaca gaaggcagag aaggaggtca cccgcatggt     2580

catcatcatg gtcatcgctt tcctgatctg ctgggtgccc tacgccagcg tggcattcta     2640

catcttcacc caccagggct ccaacttcgg tcccatcttc atgaccatcc cagcgttctt     2700

tgccaagagc gccgccatct acaaccctgt catctatatc atgatgaaca agcagttccg     2760

gaactgcatg ctcaccacca tctgctgcgg caagaaccca ctgggtgacg atgaggcctc     2820

tgctaccgtg tccaagacgg agacgagcca ggtggccccg gcctaaaagc ttatcgataa     2880

tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc     2940

ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat     3000

ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg     3060

gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg     3120

ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat     3180

tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt     3240

gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc     3300

ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa     3360

tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg     3420

ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg     3480

actcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     3540

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     3600

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     3660

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     3720

atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcatggct acgtagataa     3780

gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc     3840

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg     3900

cggcctcagt gagcgagcga gcgcgcagag ctttttgcaa aagccta                   3947


<210>  29
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE

<400>  29
aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct       60

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt      120

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg      180

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact      240

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct      300

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg      360

ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc      420

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc      480

aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt      540

cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgc                  589


<210>  30
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BstB1 restriction site and adjacent downstream 'G'

<400>  30
ttcgaag                                                                  7


<210>  31
<211>  7012
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gene therapy vector

<400>  31
cacatacgat ttaggtgaca ctatagaata cacggaatta attctagctg cgcgctcgct       60

cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct      120

cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt      180

agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg tacccagatc      240

ttccccacct agccacctgg caaactgctc cttctctcaa aggcccaaac atggcctccc      300

agactgcaac ccccaggcag tcaggccctg tctccacaac ctcacagcca ccctggacgg      360

aatctgcttc ttcccacatt tgagtcctcc tcagcccctg agctcctctg ggcagggctg      420

tttctttcca tctttgtatt cccaggggcc tgcaaataaa tgtttaatga acgaacaaga      480

gagtgaattc caattccatg caacaaggat tgggctcctg ggccctaggc tatgtgtctg      540

gcaccagaaa cggaagctgc aggttgcagc ccctgccctc atggagctcc tcctgtcaga      600

ggagtgtggg gactggatga ctccagaggt aacttgtggg ggaacgaaca ggtaaggggc      660

tgtgtgacga gatgagagac tgggagaata aaccagaaag tctctagctg tccagaggac      720

atagcacaga ggcccatggt ccctatttca aacccaggcc accagactga gctgggacct      780

tgggacagac aagtcatgca gaagttaggg gaccttctcc tcccttttcc tggatcctga      840

gtacctctcc tccctgacct caggcttcct cctagtgtca ccttggcccc tcttagaagc      900

caattaggcc ctcagtttct gcagcgggga ttaatatgat tatgaacacc cccaatctcc      960

cagatgctga ttcagccagg agcttaggag ggggaggtca ctttataagg gtctgggggg     1020

gtcagaaccc agagtcatcc ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg     1080

ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg     1140

ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt     1200

cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga     1260

gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg     1320

ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc     1380

ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc     1440

aaagaattgg atcctagctt gattgacggg aactacaaga cccgcgctga agtcaagttc     1500

gaaggtgagg aagttgatgg ggaagttcaa gagacttccc catcaacttc tttctcagtt     1560

ttttctcttt cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga     1620

ctttaaggag gattgacggg aactacaaga cccgcgctga agtcaagttc gaaggtagat     1680

gacaaaagac tcgttttcaa gagaaacgag tcttttgtca tttctcagtt ttttctcttt     1740

cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag     1800

gatatcgaat tccgccacca tgaatggcac agaaggccct aacttctacg tgcccttctc     1860

caatgcgacg ggtgtggtac gcagcccctt cgagtaccca cagtactacc tggctgagcc     1920

atggcagttc tccatgctgg ccgcctacat gtttctgctg atcgtgctgg gatttccaat     1980

taattttctg acgctctacg tcaccgtcca gcacaagaag ctgcgcacgc ctctcaacta     2040

catcctgctc aacctagccg tggctgacct cttcatggtc ctaggtggct tcaccagcac     2100

cctctacacc tctctgcatg gatacttcgt cttcgggccc acaggatgca atttggaggg     2160

cttctttgcc accctgggcg gtgaaattgc cctgtggtcc ttggtggtcc tggccatcga     2220

gcggtacgtg gtggtgtgta agcccatgag caacttccgc ttcggggaga accatgccat     2280

catgggcgtt gccttcacct gggtcatggc gctggcctgc gccgcacccc cactcgccgg     2340

ctggtccagg tacatccccg agggcctgca gtgctcgtgt ggaatcgact actacacgct     2400

caagccggag gtcaacaatg aatccttcgt gatttatatg ttcgtggtcc acttcaccat     2460

ccccatgatt atcatctttt tctgctatgg gcagctcgtc ttcaccgtca aggaggccgc     2520

tgcccagcag caggagtcag ccaccacaca gaaggcagag aaggaggtca cccgcatggt     2580

catcatcatg gtcatcgctt tcctgatctg ctgggtgccc tacgccagcg tggcattcta     2640

catcttcacc caccagggct ccaacttcgg tcccatcttc atgaccatcc cagcgttctt     2700

tgccaagagc gccgccatct acaaccctgt catctatatc atgatgaaca agcagttccg     2760

gaactgcatg ctcaccacca tctgctgcgg caagaaccca ctgggtgacg atgaggcctc     2820

tgctaccgtg tccaagacgg agacgagcca ggtggccccg gcctaaaagc ttatcgataa     2880

tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc     2940

ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat     3000

ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg     3060

gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg     3120

ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat     3180

tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt     3240

gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc     3300

ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa     3360

tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg     3420

ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg     3480

actcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     3540

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     3600

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     3660

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct     3720

atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcatggct acgtagataa     3780

gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc     3840

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg     3900

cggcctcagt gagcgagcga gcgcgcagag ctttttgcaa aagcctaggc ctccaaaaaa     3960

gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat     4020

aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg     4080

gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat     4140

acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg     4200

catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca     4260

cacattccac agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     4320

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     4380

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     4440

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     4500

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     4560

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     4620

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     4680

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     4740

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     4800

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     4860

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     4920

tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag     4980

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     5040

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     5100

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     5160

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     5220

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     5280

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     5340

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     5400

ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     5460

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     5520

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     5580

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     5640

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     5700

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     5760

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     5820

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     5880

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     5940

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     6000

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     6060

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     6120

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     6180

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     6240

ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa     6300

aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc     6360

tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga     6420

caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg     6480

gcatcagagc agattgtact gagagtgcac cattcgacgc tctcccttat gcgactcctg     6540

cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg     6600

tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac     6660

gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc     6720

ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc     6780

ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca tttccgggtg     6840

cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc aaaccgactc tgacggcagt     6900

ttacgagaga gatgataggg tctgcttcag taagccagat gctacacaat taggcttgta     6960

catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca ta             7012


<210>  32
<211>  177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward ITR

<400>  32
cacatacgat ttaggtgaca ctatagaata cacggaatta attctagctg cgcgctcgct       60

cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct      120

cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttcct         177


<210>  33
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse ITR

<400>  33
ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc       60

cgggcgacca aaggtcgccc gacgcccggg cggcctcagt gagcgagcga gcgcgcagag      120

ctttttgcaa aagccta                                                     137


<210>  34
<211>  806
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward ITR human rhodopsin promoter

<400>  34
cagatcttcc ccacctagcc acctggcaaa ctgctccttc tctcaaaggc ccaaacatgg       60

cctcccagac tgcaaccccc aggcagtcag gccctgtctc cacaacctca cagccaccct      120

ggacggaatc tgcttcttcc cacatttgag tcctcctcag cccctgagct cctctgggca      180

gggctgtttc tttccatctt tgtattccca ggggcctgca aataaatgtt taatgaacga      240

acaagagagt gaattccaat tccatgcaac aaggattggg ctcctgggcc ctaggctatg      300

tgtctggcac cagaaacgga agctgcaggt tgcagcccct gccctcatgg agctcctcct      360

gtcagaggag tgtggggact ggatgactcc agaggtaact tgtgggggaa cgaacaggta      420

aggggctgtg tgacgagatg agagactggg agaataaacc agaaagtctc tagctgtcca      480

gaggacatag cacagaggcc catggtccct atttcaaacc caggccacca gactgagctg      540

ggaccttggg acagacaagt catgcagaag ttaggggacc ttctcctccc ttttcctgga      600

tcctgagtac ctctcctccc tgacctcagg cttcctccta gtgtcacctt ggcccctctt      660

agaagccaat taggccctca gtttctgcag cggggattaa tatgattatg aacaccccca      720

atctcccaga tgctgattca gccaggagct taggaggggg aggtcacttt ataagggtct      780

gggggggtca gaacccagag tcatcc                                           806


<210>  35
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eGFP CDS

<400>  35
atgagcaagg gcgaggaact gttcactggc gtggtcccaa ttctcgtgga actggatggc       60

gatgtgaatg ggcacaaatt ttctgtcagc ggagagggtg aaggtgatgc cacatacgga      120

aagctcaccc tgaaattcat ctgcaccact ggaaagctcc ctgtgccatg gccaacactg      180

gtcactaccc tgacctatgg cgtgcagtgc ttttccagat acccagacca tatgaagcag      240

catgactttt tcaagagcgc catgcccgag ggctatgtgc aggagagaac catctttttc      300

aaagatgacg ggaactacaa gacccgcgct gaagtcaagt tcgaaggtga caccctggtg      360

aatagaatcg agctgaaggg cattgacttt aaggaggatg gaaacattct cggccacaag      420

ctggaataca actataactc ccacaatgtg tacatcatgg ccgacaagca aaagaatggc      480

atcaaggtca acttcaagat cagacacaac attgaggatg gatccgtgca gctggccgac      540

cattatcaac agaacactcc aatcggcgac ggccctgtgc tcctcccaga caaccattac      600

ctgtccaccc agtctgccct gtctaaagat cccaacgaaa agagagacca catggtcctg      660

ctggagtttg tgaccgctgc tgggatcaca catggcatgg acgagctgta caagtga         717


<210>  36
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  eGFP amino acid

<400>  36

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  37
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' ESE rich sequence

<400>  37
tgacgggaac tacaagaccc gcgctgaagt caagttcgaa g                           41


<210>  38
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' ESE rich sequence

<400>  38
gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag gat              53


<210>  39
<211>  439
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'UTR without mirtrons and additional ESE-rich sequences

<400>  39
ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg       60

ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct      120

ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa      180

agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gctgtccgcg      240

gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac      300

cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct      360

gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgg atcctagctt      420

gatatcgaat tccgccacc                                                   439


<210>  40
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ESE-rich sequence between tandem mirtrons

<400>  40
gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag gattgacggg       60

aactacaaga cccgcgctga agtcaagttc gaag                                   94


<210>  41
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 1 forward oligonucleotide

<400>  41
cgaaggtgga ccacgaacat gtagatttca agagaatcta catgttcgtg gtccctcagt       60

tttttctctt tctttt                                                       76


<210>  42
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 2 forward oligonucleotide

<400>  42
cgaaggtaga gcgtgaggaa gttgatttca agagaatcaa cttcctcacg ctttctcagt       60

tttttctctt tctttt                                                       76


<210>  43
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 3 forward oligonucleotide

<400>  43
cgaaggtgag gaagttgatg gggaagttca agagacttcc ccatcaactt ctttctcagt       60

tttttctctt tctttt                                                       76


<210>  44
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 4 forward oligonucleotide

<400>  44
cgaaggtgac gtagagcgtg aggaagttca agagacttcc tcacgctcta cgttctcagt       60

tttttctctt tctttt                                                       76


<210>  45
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 5H forward oligonucleotide

<400>  45
cgaaggtaga tgacaaaaga ctcgttttca agagaaacga gtcttttgtc atttctcagt       60

tttttctctt tctttt                                                       76


<210>  46
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 6 forward oligonucleotide

<400>  46
cgaaggtgaa gtggaccacg aacatgttca agagacatgt tcgtggtcca ctttctcagt       60

tttttctctt tctttt                                                       76


<210>  47
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 7 forward oligonucleotide

<400>  47
cgaaggtgag catgcagttc cggaacttca agagagttcc ggaactgcat gtttctcagt       60

tttttctctt tctttt                                                       76


<210>  48
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 1 reverse oligonucleotide

<400>  48
cgaaaagaaa gagaaaaaac tgagggacca cgaacatgta gattctcttg aaatctacat       60

gttcgtggtc cacctt                                                       76


<210>  49
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 2 reverse oligonucleotide

<400>  49
cgaaaagaaa gagaaaaaac tgagaaagcg tgaggaagtt gattctcttg aaatcaactt       60

cctcacgctc tacctt                                                       76


<210>  50
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 3 reverse oligonucleotide

<400>  50
cgaaaagaaa gagaaaaaac tgagaaagaa gttgatgggg aagtctcttg aacttcccca       60

tcaacttcct cacctt                                                       76


<210>  51
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 4 reverse oligonucleotide

<400>  51
cgaaaagaaa gagaaaaaac tgagaacgta gagcgtgagg aagtctcttg aacttcctca       60

cgctctacgt cacctt                                                       76


<210>  52
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 5H reverse oligonucleotide

<400>  52
cgaaaagaaa gagaaaaaac tgagaaatga caaaagactc gtttctcttg aaaacgagtc       60

ttttgtcatc tacctt                                                       76


<210>  53
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 6 reverse oligonucleotide

<400>  53
cgaaaagaaa gagaaaaaac tgagaaagtg gaccacgaac atgtctcttg aacatgttcg       60

tggtccactt cacctt                                                       76


<210>  54
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 7 reverse oligonucleotide

<400>  54
cgaaaagaaa gagaaaaaac tgagaaacat gcagttccgg aactctcttg aagttccgga       60

actgcatgct cacctt                                                       76


<210>  55
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 5M

<400>  55
gtagatgaca aaggattcgt tttcaagaga aacgaatcct ttgtcatttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  56
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 5M forward oligonucleotide

<400>  56
cgaaggtaga tgacaaagga ttcgttttca agagaaacga atcctttgtc atttctcagt       60

tttttctctt tctttt                                                       76


<210>  57
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron 5M reverse oligonucleotide

<400>  57
cgaaaagaaa gagaaaaaac tgagaaatga caaaggattc gtttctcttg aaaacgaatc       60

ctttgtcatc tacctt                                                       76


<210>  58
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron M2U-M2

<400>  58
atagagcgtg aggaagttga tttcaagaga atcaacttcc tcacgctttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  59
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron M2U-M2 forward oligonucleotide

<400>  59
cgaagataga gcgtgaggaa gttgatttca agagaatcaa cttcctcacg ctttctcagt       60

tttttctctt tctttt                                                       76


<210>  60
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron M2U-M2 reverse oligonucleotide

<400>  60
cgaaaagaaa gagaaaaaac tgagaaagcg tgaggaagtt gattctcttg aaatcaactt       60

cctcacgctc tatctt                                                       76


<210>  61
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron Scr-M2

<400>  61
gtagagatgt ggttgaggat tttcaagaga aatcctcaac cacatctttc tcagtttttt       60

ctctttcttt tcgaag                                                       76


<210>  62
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron Scr-M2 forward oligonucleotide

<400>  62
cgaaggtaga gatgtggttg aggattttca agagaaatcc tcaaccacat ctttctcagt       60

tttttctctt tctttt                                                       76


<210>  63
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mirtron Scr-M2 reverse oligonucleotide

<400>  63
cgaaaagaaa gagaaaaaac tgagaaagat gtggttgagg atttctcttg aaaatcctca       60

accacatctc tacctt                                                       76


<210>  64
<211>  6417
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  vector AAV2/8Y733F.RHOp.Ex/Int.M3/M5.RHOM3/5R

<400>  64
cacatacgat ttaggtgaca ctatagaata cacggaatta attctagctg cgcgctcgct       60

cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct      120

cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt      180

agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg tacccagatc      240

ttccccacct agccacctgg caaactgctc cttctctcaa aggcccaaac atggcctccc      300

agactgcaac ccccaggcag tcaggccctg tctccacaac ctcacagcca ccctggacgg      360

aatctgcttc ttcccacatt tgagtcctcc tcagcccctg agctcctctg ggcagggctg      420

tttctttcca tctttgtatt cccaggggcc tgcaaataaa tgtttaatga acgaacaaga      480

gagtgaattc caattccatg caacaaggat tgggctcctg ggccctaggc tatgtgtctg      540

gcaccagaaa cggaagctgc aggttgcagc ccctgccctc atggagctcc tcctgtcaga      600

ggagtgtggg gactggatga ctccagaggt aacttgtggg ggaacgaaca ggtaaggggc      660

tgtgtgacga gatgagagac tgggagaata aaccagaaag tctctagctg tccagaggac      720

atagcacaga ggcccatggt ccctatttca aacccaggcc accagactga gctgggacct      780

tgggacagac aagtcatgca gaagttaggg gaccttctcc tcccttttcc tggatcctga      840

gtacctctcc tccctgacct caggcttcct cctagtgtca ccttggcccc tcttagaagc      900

caattaggcc ctcagtttct gcagcgggga ttaatatgat tatgaacacc cccaatctcc      960

cagatgctga ttcagccagg agcttaggag ggggaggtca ctttataagg gtctgggggg     1020

gtcagaaccc agagtcatcc ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg     1080

ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg     1140

ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt     1200

cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga     1260

gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg     1320

ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc     1380

ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc     1440

aaagaattgg atcctagctt gattgacggg aactacaaga cccgcgctga agtcaagttc     1500

gaaggtgagg aagttgatgg ggaagttcaa gagacttccc catcaacttc tttctcagtt     1560

ttttctcttt cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga     1620

ctttaaggag gattgacggg aactacaaga cccgcgctga agtcaagttc gaaggtagat     1680

gacaaaagac tcgttttcaa gagaaacgag tcttttgtca tttctcagtt ttttctcttt     1740

cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag     1800

gatatcgaat tccgccacca tgaatggcac agaaggccct aacttctacg tgcccttctc     1860

caatgcgacg ggtgtggtac gcagcccctt cgagtaccca cagtactacc tggctgagcc     1920

atggcagttc tccatgctgg ccgcctacat gtttctgctg atcgtgctgg gatttccaat     1980

taattttctg acgctctacg tcaccgtcca gcacaagaag ctgcgcacgc ctctcaacta     2040

catcctgctc aacctagccg tggctgacct cttcatggtc ctaggtggct tcaccagcac     2100

cctctacacc tctctgcatg gatacttcgt cttcgggccc acaggatgca atttggaggg     2160

cttctttgcc accctgggcg gtgaaattgc cctgtggtcc ttggtggtcc tggccatcga     2220

gcggtacgtg gtggtgtgta agcccatgag caacttccgc ttcggggaga accatgccat     2280

catgggcgtt gccttcacct gggtcatggc gctggcctgc gccgcacccc cactcgccgg     2340

ctggtccagg tacatccccg agggcctgca gtgctcgtgt ggaatcgact actacacgct     2400

caagccggag gtcaacaatg aatccttcgt gatttatatg ttcgtggtcc acttcaccat     2460

ccccatgatt atcatctttt tctgctatgg gcagctcgtc ttcaccgtca aggaggccgc     2520

tgcccagcag caggagtcag ccaccacaca gaaggcagag aaggaggtca cccgcatggt     2580

catcatcatg gtcatcgctt tcctgatctg ctgggtgccc tacgccagcg tggcattcta     2640

catcttcacc caccagggct ccaacttcgg tcccatcttc atgaccatcc cagcgttctt     2700

tgccaagagc gccgccatct acaaccctgt catctatatc atgatgaaca agcagttccg     2760

gaactgcatg ctcaccacca tctgctgcgg caagaaccca ctgggtgacg atgaggcctc     2820

tgctaccgtg tccaagacgg agacgagcca ggtggccccg gcctaaaagc ttatcgatac     2880

cgtcgactcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc     2940

cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa     3000

atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg     3060

ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg     3120

gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagca tggctacgta     3180

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc     3240

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc     3300

ccgggcggcc tcagtgagcg agcgagcgcg cagagctttt tgcaaaagcc taggcctcca     3360

aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca     3420

taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg     3480

ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt     3540

tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg     3600

agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac     3660

tgacacacat tccacagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc     3720

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc     3780

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata     3840

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg     3900

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     3960

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     4020

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     4080

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     4140

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     4200

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     4260

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     4320

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     4380

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     4440

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     4500

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     4560

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     4620

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     4680

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     4740

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     4800

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     4860

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     4920

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     4980

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     5040

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     5100

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     5160

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     5220

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     5280

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     5340

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     5400

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     5460

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     5520

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     5580

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     5640

catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct     5700

ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa     5760

acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga     5820

gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact     5880

atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac     5940

tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg     6000

aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata     6060

cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg     6120

atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg     6180

cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc     6240

gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg     6300

gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc     6360

ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcata        6417


<210>  65
<211>  6417
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAV2/8Y733F.RHOp.Ex/Int.M3/M5M.RHOM3/5R

<400>  65
cacatacgat ttaggtgaca ctatagaata cacggaatta attctagctg cgcgctcgct       60

cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct      120

cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt      180

agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagg tacccagatc      240

ttccccacct agccacctgg caaactgctc cttctctcaa aggcccaaac atggcctccc      300

agactgcaac ccccaggcag tcaggccctg tctccacaac ctcacagcca ccctggacgg      360

aatctgcttc ttcccacatt tgagtcctcc tcagcccctg agctcctctg ggcagggctg      420

tttctttcca tctttgtatt cccaggggcc tgcaaataaa tgtttaatga acgaacaaga      480

gagtgaattc caattccatg caacaaggat tgggctcctg ggccctaggc tatgtgtctg      540

gcaccagaaa cggaagctgc aggttgcagc ccctgccctc atggagctcc tcctgtcaga      600

ggagtgtggg gactggatga ctccagaggt aacttgtggg ggaacgaaca ggtaaggggc      660

tgtgtgacga gatgagagac tgggagaata aaccagaaag tctctagctg tccagaggac      720

atagcacaga ggcccatggt ccctatttca aacccaggcc accagactga gctgggacct      780

tgggacagac aagtcatgca gaagttaggg gaccttctcc tcccttttcc tggatcctga      840

gtacctctcc tccctgacct caggcttcct cctagtgtca ccttggcccc tcttagaagc      900

caattaggcc ctcagtttct gcagcgggga ttaatatgat tatgaacacc cccaatctcc      960

cagatgctga ttcagccagg agcttaggag ggggaggtca ctttataagg gtctgggggg     1020

gtcagaaccc agagtcatcc ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg     1080

ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg     1140

ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt     1200

cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga     1260

gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg     1320

ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc     1380

ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc     1440

aaagaattgg atcctagctt gattgacggg aactacaaga cccgcgctga agtcaagttc     1500

gaaggtgagg aagttgatgg ggaagttcaa gagacttccc catcaacttc tttctcagtt     1560

ttttctcttt cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga     1620

ctttaaggag gattgacggg aactacaaga cccgcgctga agtcaagttc gaaggtagat     1680

gacaaaggat tcgttttcaa gagaaacgaa tcctttgtca tttctcagtt ttttctcttt     1740

cttttcgaag gtgacaccct ggtgaataga atcgagctga agggcattga ctttaaggag     1800

gatatcgaat tccgccacca tgaatggcac agaaggccct aacttctacg tgcccttctc     1860

caatgcgacg ggtgtggtac gcagcccctt cgagtaccca cagtactacc tggctgagcc     1920

atggcagttc tccatgctgg ccgcctacat gtttctgctg atcgtgctgg gatttccaat     1980

taattttctg acgctctacg tcaccgtcca gcacaagaag ctgcgcacgc ctctcaacta     2040

catcctgctc aacctagccg tggctgacct cttcatggtc ctaggtggct tcaccagcac     2100

cctctacacc tctctgcatg gatacttcgt cttcgggccc acaggatgca atttggaggg     2160

cttctttgcc accctgggcg gtgaaattgc cctgtggtcc ttggtggtcc tggccatcga     2220

gcggtacgtg gtggtgtgta agcccatgag caacttccgc ttcggggaga accatgccat     2280

catgggcgtt gccttcacct gggtcatggc gctggcctgc gccgcacccc cactcgccgg     2340

ctggtccagg tacatccccg agggcctgca gtgctcgtgt ggaatcgact actacacgct     2400

caagccggag gtcaacaatg aatccttcgt gatttatatg ttcgtggtcc acttcaccat     2460

ccccatgatt atcatctttt tctgctatgg gcagctcgtc ttcaccgtca aggaggccgc     2520

tgcccagcag caggagtcag ccaccacaca gaaggcagag aaggaggtca cccgcatggt     2580

catcatcatg gtcatcgctt tcctgatctg ctgggtgccc tacgccagcg tggcattcta     2640

catcttcacc caccagggct ccaacttcgg tcccatcttc atgaccatcc cagcgttctt     2700

tgccaagagc gccgccatct acaaccctgt catctatatc atgatgaaca agcagttccg     2760

gaactgcatg ctcaccacca tctgctgcgg caagaaccca ctgggtgacg atgaggcctc     2820

tgctaccgtg tccaagacgg agacgagcca ggtggccccg gcctaaaagc ttatcgatac     2880

cgtcgactcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc     2940

cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa     3000

atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg     3060

ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg     3120

gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagca tggctacgta     3180

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc     3240

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc     3300

ccgggcggcc tcagtgagcg agcgagcgcg cagagctttt tgcaaaagcc taggcctcca     3360

aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca     3420

taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg     3480

ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt     3540

tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg     3600

agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac     3660

tgacacacat tccacagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc     3720

gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc     3780

ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata     3840

acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg     3900

cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct     3960

caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa     4020

gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc     4080

tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt     4140

aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg     4200

ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg     4260

cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct     4320

tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc     4380

tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     4440

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     4500

aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt     4560

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     4620

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     4680

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     4740

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     4800

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     4860

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     4920

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     4980

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     5040

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     5100

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     5160

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     5220

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     5280

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     5340

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     5400

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     5460

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     5520

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     5580

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     5640

catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct     5700

ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa     5760

acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga     5820

gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact     5880

atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac     5940

tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg     6000

aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata     6060

cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg     6120

atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg     6180

cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc     6240

gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg     6300

gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc     6360

ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcata        6417


