                         SEQUENCE LISTING

<110>  United Kingdom Research and Innovation
 
<120>  METHODS AND COMPOSITIONS

<130>  |P10564GBWO|

<140>  PCT/GB2019/053023
<141>  2019-10-24

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  72
<212>  DNA
<213>  Methanosarcina barkeri

<400>  1
gggaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg       60

gggtttccgc ca                                                           72


<210>  2
<211>  72
<212>  DNA
<213>  Methanosarcina barkeri

<400>  2
ggaaacctga tcatgtagat cgaatggact ctaaatccgt tcagccgggt tagattcccg       60

gggtttccgc ca                                                           72


<210>  3
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DAPRS synthetase

<400>  3

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Cys Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Gln Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Phe Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Cys Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  4
<211>  419
<212>  PRT
<213>  Methanosarcina barkeri

<400>  4

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  5
<211>  419
<212>  PRT
<213>  Methanosarcina barkeri

<400>  5

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His Tyr Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Gly Lys Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Pro Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asp Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Pro Val Pro Thr Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Ile Ala Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Asp Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Asp Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ser Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  6
<211>  454
<212>  PRT
<213>  Methanosarcina mazeii

<400>  6

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  7
<211>  443
<212>  PRT
<213>  Methanosarcina acetivorans

<400>  7

Met Asp Lys Lys Pro Leu Asp Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Met Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Glu Arg Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Arg His Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Lys Thr Ser Glu Glu Lys Thr Thr Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Arg Val Arg Lys Ala Met Pro Lys Ser Val Ala 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Ala Thr Ala Gln Val Pro Leu Ser Gly 
        115                 120                 125             


Ser Lys Pro Ala Pro Ala Thr Pro Val Ser Ala Pro Ala Gln Ala Pro 
    130                 135                 140                 


Ala Pro Ser Thr Gly Ser Ala Ser Ala Thr Ser Ala Ser Ala Gln Arg 
145                 150                 155                 160 


Met Ala Asn Ser Ala Ala Ala Pro Ala Ala Pro Val Pro Thr Ser Ala 
                165                 170                 175     


Pro Ala Leu Thr Lys Gly Gln Leu Asp Arg Leu Glu Gly Leu Leu Ser 
            180                 185                 190         


Pro Lys Asp Glu Ile Ser Leu Asp Ser Glu Lys Pro Phe Arg Glu Leu 
        195                 200                 205             


Glu Ser Glu Leu Leu Ser Arg Arg Lys Lys Asp Leu Lys Arg Ile Tyr 
    210                 215                 220                 


Ala Glu Glu Arg Glu Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr 
225                 230                 235                 240 


Lys Phe Phe Val Asp Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu 
                245                 250                 255     


Ile Pro Ala Glu Tyr Val Glu Arg Met Gly Ile Asn Ser Asp Thr Glu 
            260                 265                 270         


Leu Ser Lys Gln Val Phe Arg Ile Asp Lys Asn Phe Cys Leu Arg Pro 
        275                 280                 285             


Met Leu Ala Pro Asn Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala 
    290                 295                 300                 


Leu Pro Asp Pro Ile Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys 
305                 310                 315                 320 


Glu Ser Asp Gly Lys Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe 
                325                 330                 335     


Cys Gln Met Gly Ser Gly Cys Thr Arg Glu Asn Leu Glu Ala Ile Ile 
            340                 345                 350         


Thr Glu Phe Leu Asn His Leu Gly Ile Asp Phe Glu Ile Ile Gly Asp 
        355                 360                 365             


Ser Cys Met Val Tyr Gly Asn Thr Leu Asp Val Met His Asp Asp Leu 
    370                 375                 380                 


Glu Leu Ser Ser Ala Val Val Gly Pro Val Pro Leu Asp Arg Glu Trp 
385                 390                 395                 400 


Gly Ile Asp Lys Pro Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu 
                405                 410                 415     


Leu Lys Val Met His Gly Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser 
            420                 425                 430         


Glu Ser Tyr Tyr Asn Gly Ile Ser Thr Asn Leu 
        435                 440             


<210>  8
<211>  478
<212>  PRT
<213>  Methanosarcina thermophila

<400>  8

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Lys Leu His Lys Ile Arg His His Glu Val Ser 
            20                  25                  30          


Lys Arg Lys Ile Tyr Ile Glu Met Glu Cys Gly Glu Arg Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Ala Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Ile Cys Lys His Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Arg Thr Asn Glu Asp Lys Ser Asn Ala Lys Val Thr 
                85                  90                  95      


Val Val Ser Ala Pro Lys Ile Arg Lys Val Met Pro Lys Ser Val Ala 
            100                 105                 110         


Arg Thr Pro Lys Pro Leu Glu Asn Thr Ala Pro Val Gln Thr Leu Pro 
        115                 120                 125             


Ser Glu Ser Gln Pro Ala Pro Thr Thr Pro Ile Ser Ala Ser Thr Thr 
    130                 135                 140                 


Ala Pro Ala Ser Thr Ser Thr Thr Ala Pro Ala Pro Ala Ser Thr Thr 
145                 150                 155                 160 


Ala Pro Ala Pro Ala Ser Thr Thr Ala Pro Ala Ser Ala Ser Thr Thr 
                165                 170                 175     


Ile Ser Thr Ser Ala Met Pro Ala Ser Thr Ser Ala Gln Gly Thr Thr 
            180                 185                 190         


Lys Phe Asn Tyr Ile Ser Gly Gly Phe Pro Arg Pro Ile Pro Val Gln 
        195                 200                 205             


Ala Ser Ala Pro Ala Leu Thr Lys Ser Gln Ile Asp Arg Leu Gln Gly 
    210                 215                 220                 


Leu Leu Ser Pro Lys Asp Glu Ile Ser Leu Asp Ser Gly Thr Pro Phe 
225                 230                 235                 240 


Arg Lys Leu Glu Ser Glu Leu Leu Ser Arg Arg Arg Lys Asp Leu Lys 
                245                 250                 255     


Gln Ile Tyr Ala Glu Glu Arg Glu His Tyr Leu Gly Lys Leu Glu Arg 
            260                 265                 270         


Glu Ile Thr Lys Phe Phe Val Asp Arg Gly Phe Leu Glu Ile Lys Ser 
        275                 280                 285             


Pro Ile Leu Ile Pro Met Glu Tyr Ile Glu Arg Met Gly Ile Asp Asn 
    290                 295                 300                 


Asp Lys Glu Leu Ser Lys Gln Ile Phe Arg Val Asp Asn Asn Phe Cys 
305                 310                 315                 320 


Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Asn Tyr Leu Arg Lys Leu 
                325                 330                 335     


Asn Arg Ala Leu Pro Asp Pro Ile Lys Ile Phe Glu Ile Gly Pro Cys 
            340                 345                 350         


Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu Glu Glu Phe Thr Met 
        355                 360                 365             


Leu Asn Phe Cys Gln Met Gly Ser Gly Cys Thr Arg Glu Asn Leu Glu 
    370                 375                 380                 


Ala Ile Ile Lys Asp Phe Leu Asp Tyr Leu Gly Ile Asp Phe Glu Ile 
385                 390                 395                 400 


Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr Leu Asp Val Met His 
                405                 410                 415     


Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly Pro Val Pro Met Asp 
            420                 425                 430         


Arg Asp Trp Gly Ile Asn Lys Pro Trp Ile Gly Ala Gly Phe Gly Leu 
        435                 440                 445             


Glu Arg Leu Leu Lys Val Met His Asn Phe Lys Asn Ile Lys Arg Ala 
    450                 455                 460                 


Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser Thr Asn Leu 
465                 470                 475             


<210>  9
<211>  416
<212>  PRT
<213>  Methanococcoides burtonii

<400>  9

Met Glu Lys Gln Leu Leu Asp Val Leu Val Glu Leu Asn Gly Val Trp 
1               5                   10                  15      


Leu Ser Arg Ser Gly Leu Leu His Gly Ile Arg Asn Phe Glu Ile Thr 
            20                  25                  30          


Thr Lys His Ile His Ile Glu Thr Asp Cys Gly Ala Arg Phe Thr Val 
        35                  40                  45              


Arg Asn Ser Arg Ser Ser Arg Ser Ala Arg Ser Leu Arg His Asn Lys 
    50                  55                  60                  


Tyr Arg Lys Pro Cys Lys Arg Cys Arg Pro Ala Asp Glu Gln Ile Asp 
65                  70                  75                  80  


Arg Phe Val Lys Lys Thr Phe Lys Glu Lys Arg Gln Thr Val Ser Val 
                85                  90                  95      


Phe Ser Ser Pro Lys Lys His Val Pro Lys Lys Pro Lys Val Ala Val 
            100                 105                 110         


Ile Lys Ser Phe Ser Ile Ser Thr Pro Ser Pro Lys Glu Ala Ser Val 
        115                 120                 125             


Ser Asn Ser Ile Pro Thr Pro Ser Ile Ser Val Val Lys Asp Glu Val 
    130                 135                 140                 


Lys Val Pro Glu Val Lys Tyr Thr Pro Ser Gln Ile Glu Arg Leu Lys 
145                 150                 155                 160 


Thr Leu Met Ser Pro Asp Asp Lys Ile Pro Ile Gln Asp Glu Leu Pro 
                165                 170                 175     


Glu Phe Lys Val Leu Glu Lys Glu Leu Ile Gln Arg Arg Arg Asp Asp 
            180                 185                 190         


Leu Lys Lys Met Tyr Glu Glu Asp Arg Glu Asp Arg Leu Gly Lys Leu 
        195                 200                 205             


Glu Arg Asp Ile Thr Glu Phe Phe Val Asp Arg Gly Phe Leu Glu Ile 
    210                 215                 220                 


Lys Ser Pro Ile Met Ile Pro Phe Glu Tyr Ile Glu Arg Met Gly Ile 
225                 230                 235                 240 


Asp Lys Asp Asp His Leu Asn Lys Gln Ile Phe Arg Val Asp Glu Ser 
                245                 250                 255     


Met Cys Leu Arg Pro Met Leu Ala Pro Cys Leu Tyr Asn Tyr Leu Arg 
            260                 265                 270         


Lys Leu Asp Lys Val Leu Pro Asp Pro Ile Arg Ile Phe Glu Ile Gly 
        275                 280                 285             


Pro Cys Tyr Arg Lys Glu Ser Asp Gly Ser Ser His Leu Glu Glu Phe 
    290                 295                 300                 


Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr Arg Glu Asn 
305                 310                 315                 320 


Met Glu Ala Leu Ile Asp Glu Phe Leu Glu His Leu Gly Ile Glu Tyr 
                325                 330                 335     


Glu Ile Glu Ala Asp Asn Cys Met Val Tyr Gly Asp Thr Ile Asp Ile 
            340                 345                 350         


Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly Pro Ile Pro 
        355                 360                 365             


Leu Asp Arg Glu Trp Gly Val Asn Lys Pro Trp Met Gly Ala Gly Phe 
    370                 375                 380                 


Gly Leu Glu Arg Leu Leu Lys Val Arg His Asn Tyr Thr Asn Ile Arg 
385                 390                 395                 400 


Arg Ala Ser Arg Ser Glu Leu Tyr Tyr Asn Gly Ile Asn Thr Asn Leu 
                405                 410                 415     


<210>  10
<211>  279
<212>  PRT
<213>  Desulfitobacterium hafniense

<400>  10

Met Ser Ser Phe Trp Thr Lys Val Gln Tyr Gln Arg Leu Lys Glu Leu 
1               5                   10                  15      


Asn Ala Ser Gly Glu Gln Leu Glu Met Gly Phe Ser Asp Ala Leu Ser 
            20                  25                  30          


Arg Asp Arg Ala Phe Gln Gly Ile Glu His Gln Leu Met Ser Gln Gly 
        35                  40                  45              


Lys Arg His Leu Glu Gln Leu Arg Thr Val Lys His Arg Pro Ala Leu 
    50                  55                  60                  


Leu Glu Leu Glu Glu Gly Leu Ala Lys Ala Leu His Gln Gln Gly Phe 
65                  70                  75                  80  


Val Gln Val Val Thr Pro Thr Ile Ile Thr Lys Ser Ala Leu Ala Lys 
                85                  90                  95      


Met Thr Ile Gly Glu Asp His Pro Leu Phe Ser Gln Val Phe Trp Leu 
            100                 105                 110         


Asp Gly Lys Lys Cys Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Thr 
        115                 120                 125             


Leu Trp Arg Glu Leu Glu Arg Leu Trp Asp Lys Pro Ile Arg Ile Phe 
    130                 135                 140                 


Glu Ile Gly Thr Cys Tyr Arg Lys Glu Ser Gln Gly Ala Gln His Leu 
145                 150                 155                 160 


Asn Glu Phe Thr Met Leu Asn Leu Thr Glu Leu Gly Thr Pro Leu Glu 
                165                 170                 175     


Glu Arg His Gln Arg Leu Glu Asp Met Ala Arg Trp Val Leu Glu Ala 
            180                 185                 190         


Ala Gly Ile Arg Glu Phe Glu Leu Val Thr Glu Ser Ser Val Val Tyr 
        195                 200                 205             


Gly Asp Thr Val Asp Val Met Lys Gly Asp Leu Glu Leu Ala Ser Gly 
    210                 215                 220                 


Ala Met Gly Pro His Phe Leu Asp Glu Lys Trp Glu Ile Val Asp Pro 
225                 230                 235                 240 


Trp Val Gly Leu Gly Phe Gly Leu Glu Arg Leu Leu Met Ile Arg Glu 
                245                 250                 255     


Gly Thr Gln His Val Gln Ser Met Ala Arg Ser Leu Ser Tyr Leu Asp 
            260                 265                 270         


Gly Val Arg Leu Asn Ile Asn 
        275                 


<210>  11
<211>  312
<212>  PRT
<213>  Desulfitobacterium hafniense

<400>  11

Met Asp Arg Ile Asp His Thr Asp Ser Lys Phe Val Gln Ala Gly Glu 
1               5                   10                  15      


Thr Pro Val Leu Pro Ala Thr Phe Met Phe Leu Thr Arg Arg Asp Pro 
            20                  25                  30          


Pro Leu Ser Ser Phe Trp Thr Lys Val Gln Tyr Gln Arg Leu Lys Glu 
        35                  40                  45              


Leu Asn Ala Ser Gly Glu Gln Leu Glu Met Gly Phe Ser Asp Ala Leu 
    50                  55                  60                  


Ser Arg Asp Arg Ala Phe Gln Gly Ile Glu His Gln Leu Met Ser Gln 
65                  70                  75                  80  


Gly Lys Arg His Leu Glu Gln Leu Arg Thr Val Lys His Arg Pro Ala 
                85                  90                  95      


Leu Leu Glu Leu Glu Glu Gly Leu Ala Lys Ala Leu His Gln Gln Gly 
            100                 105                 110         


Phe Val Gln Val Val Thr Pro Thr Ile Ile Thr Lys Ser Ala Leu Ala 
        115                 120                 125             


Lys Met Thr Ile Gly Glu Asp His Pro Leu Phe Ser Gln Val Phe Trp 
    130                 135                 140                 


Leu Asp Gly Lys Lys Cys Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr 
145                 150                 155                 160 


Thr Leu Trp Arg Glu Leu Glu Arg Leu Trp Asp Lys Pro Ile Arg Ile 
                165                 170                 175     


Phe Glu Ile Gly Thr Cys Tyr Arg Lys Glu Ser Gln Gly Ala Gln His 
            180                 185                 190         


Leu Asn Glu Phe Thr Met Leu Asn Leu Thr Glu Leu Gly Thr Pro Leu 
        195                 200                 205             


Glu Glu Arg His Gln Arg Leu Glu Asp Met Ala Arg Trp Val Leu Glu 
    210                 215                 220                 


Ala Ala Gly Ile Arg Glu Phe Glu Leu Val Thr Glu Ser Ser Val Val 
225                 230                 235                 240 


Tyr Gly Asp Thr Val Asp Val Met Lys Gly Asp Leu Glu Leu Ala Ser 
                245                 250                 255     


Gly Ala Met Gly Pro His Phe Leu Asp Glu Lys Trp Glu Ile Val Asp 
            260                 265                 270         


Pro Trp Val Gly Leu Gly Phe Gly Leu Glu Arg Leu Leu Met Ile Arg 
        275                 280                 285             


Glu Gly Thr Gln His Val Gln Ser Met Ala Arg Ser Leu Ser Tyr Leu 
    290                 295                 300                 


Asp Gly Val Arg Leu Asn Ile Asn 
305                 310         


<210>  12
<211>  288
<212>  PRT
<213>  Desulfitobacterium hafniense

<400>  12

Met Phe Leu Thr Arg Arg Asp Pro Pro Leu Ser Ser Phe Trp Thr Lys 
1               5                   10                  15      


Val Gln Tyr Gln Arg Leu Lys Glu Leu Asn Ala Ser Gly Glu Gln Leu 
            20                  25                  30          


Glu Met Gly Phe Ser Asp Ala Leu Ser Arg Asp Arg Ala Phe Gln Gly 
        35                  40                  45              


Ile Glu His Gln Leu Met Ser Gln Gly Lys Arg His Leu Glu Gln Leu 
    50                  55                  60                  


Arg Thr Val Lys His Arg Pro Ala Leu Leu Glu Leu Glu Glu Lys Leu 
65                  70                  75                  80  


Ala Lys Ala Leu His Gln Gln Gly Phe Val Gln Val Val Thr Pro Thr 
                85                  90                  95      


Ile Ile Thr Lys Ser Ala Leu Ala Lys Met Thr Ile Gly Glu Asp His 
            100                 105                 110         


Pro Leu Phe Ser Gln Val Phe Trp Leu Asp Gly Lys Lys Cys Leu Arg 
        115                 120                 125             


Pro Met Leu Ala Pro Asn Leu Tyr Thr Leu Trp Arg Glu Leu Glu Arg 
    130                 135                 140                 


Leu Trp Asp Lys Pro Ile Arg Ile Phe Glu Ile Gly Thr Cys Tyr Arg 
145                 150                 155                 160 


Lys Glu Ser Gln Gly Ala Gln His Leu Asn Glu Phe Thr Met Leu Asn 
                165                 170                 175     


Leu Thr Glu Leu Gly Thr Pro Leu Glu Glu Arg His Gln Arg Leu Glu 
            180                 185                 190         


Asp Met Ala Arg Trp Val Leu Glu Ala Ala Gly Ile Arg Glu Phe Glu 
        195                 200                 205             


Leu Val Thr Glu Ser Ser Val Val Tyr Gly Asp Thr Val Asp Val Met 
    210                 215                 220                 


Lys Gly Asp Leu Glu Leu Ala Ser Gly Ala Met Gly Pro His Phe Leu 
225                 230                 235                 240 


Asp Glu Lys Trp Glu Ile Phe Asp Pro Trp Val Gly Leu Gly Phe Gly 
                245                 250                 255     


Leu Glu Arg Leu Leu Met Ile Arg Glu Gly Thr Gln His Val Gln Ser 
            260                 265                 270         


Met Ala Arg Ser Leu Ser Tyr Leu Asp Gly Val Arg Leu Asn Ile Asn 
        275                 280                 285             


<210>  13
<211>  277
<212>  PRT
<213>  Desulfotomaculum acetoxidans

<400>  13

Met Ser Phe Leu Trp Thr Val Ser Gln Gln Lys Arg Leu Ser Glu Leu 
1               5                   10                  15      


Asn Ala Ser Glu Glu Glu Lys Asn Met Ser Phe Ser Ser Thr Ser Asp 
            20                  25                  30          


Arg Glu Ala Ala Tyr Lys Arg Val Glu Met Arg Leu Ile Asn Glu Ser 
        35                  40                  45              


Lys Gln Arg Leu Asn Lys Leu Arg His Glu Thr Arg Pro Ala Ile Cys 
    50                  55                  60                  


Ala Leu Glu Asn Arg Leu Ala Ala Ala Leu Arg Gly Ala Gly Phe Val 
65                  70                  75                  80  


Gln Val Ala Thr Pro Val Ile Leu Ser Lys Lys Leu Leu Gly Lys Met 
                85                  90                  95      


Thr Ile Thr Asp Glu His Ala Leu Phe Ser Gln Val Phe Trp Ile Glu 
            100                 105                 110         


Glu Asn Lys Cys Leu Arg Pro Met Leu Ala Pro Asn Leu Tyr Tyr Ile 
        115                 120                 125             


Leu Lys Asp Leu Leu Arg Leu Trp Glu Lys Pro Val Arg Ile Phe Glu 
    130                 135                 140                 


Ile Gly Ser Cys Phe Arg Lys Glu Ser Gln Gly Ser Asn His Leu Asn 
145                 150                 155                 160 


Glu Phe Thr Met Leu Asn Leu Val Glu Trp Gly Leu Pro Glu Glu Gln 
                165                 170                 175     


Arg Gln Lys Arg Ile Ser Glu Leu Ala Lys Leu Val Met Asp Glu Thr 
            180                 185                 190         


Gly Ile Asp Glu Tyr His Leu Glu His Ala Glu Ser Val Val Tyr Gly 
        195                 200                 205             


Glu Thr Val Asp Val Met His Arg Asp Ile Glu Leu Gly Ser Gly Ala 
    210                 215                 220                 


Leu Gly Pro His Phe Leu Asp Gly Arg Trp Gly Val Val Gly Pro Trp 
225                 230                 235                 240 


Val Gly Ile Gly Phe Gly Leu Glu Arg Leu Leu Met Val Glu Gln Gly 
                245                 250                 255     


Gly Gln Asn Val Arg Ser Met Gly Lys Ser Leu Thr Tyr Leu Asp Gly 
            260                 265                 270         


Val Arg Leu Asn Ile 
        275         


<210>  14
<211>  419
<212>  PRT
<213>  Methanosarcina barkeri

<400>  14

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His Tyr Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Gly Lys Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Pro Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asp Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Pro Val Pro Thr Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Ile Ala Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Asp Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Asp Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ser Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  15
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial synthetase

<400>  15

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  16
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AcKRS synthetase

<400>  16

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Gly Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Val Ala Pro Thr Ile Phe Asn 
            260                 265                 270         


Tyr Ala Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Phe Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  17
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCKRS synthetase

<400>  17

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Phe Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ser Pro Thr Leu Cys Asn 
            260                 265                 270         


Tyr Met Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  18
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  artificial synthetase

<400>  18

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ala Pro Asn 
    290                 295                 300                 


Leu Tyr Asn Tyr Leu Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  19
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mm-ACKRS synthetase

<400>  19

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Met Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Val Ala Pro Asn 
    290                 295                 300                 


Ile Phe Asn Tyr Ala Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Phe Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  20
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mm-PCKRS synthetase

<400>  20

Met Asp Lys Lys Pro Leu Asn Thr Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Ile His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Ser Arg Thr Ala Arg Ala Leu Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Leu Asn 
65                  70                  75                  80  


Lys Phe Leu Thr Lys Ala Asn Glu Asp Gln Thr Ser Val Lys Val Lys 
                85                  90                  95      


Val Val Ser Ala Pro Thr Arg Thr Lys Lys Ala Met Pro Lys Ser Val 
            100                 105                 110         


Ala Arg Ala Pro Lys Pro Leu Glu Asn Thr Glu Ala Ala Gln Ala Gln 
        115                 120                 125             


Pro Ser Gly Ser Lys Phe Ser Pro Ala Ile Pro Val Ser Thr Gln Glu 
    130                 135                 140                 


Ser Val Ser Val Pro Ala Ser Val Ser Thr Ser Ile Ser Ser Ile Ser 
145                 150                 155                 160 


Thr Gly Ala Thr Ala Ser Ala Leu Val Lys Gly Asn Thr Asn Pro Ile 
                165                 170                 175     


Thr Ser Met Ser Ala Pro Val Gln Ala Ser Ala Pro Ala Leu Thr Lys 
            180                 185                 190         


Ser Gln Thr Asp Arg Leu Glu Val Leu Leu Asn Pro Lys Asp Glu Ile 
        195                 200                 205             


Ser Leu Asn Ser Gly Lys Pro Phe Arg Glu Leu Glu Ser Glu Leu Leu 
    210                 215                 220                 


Ser Arg Arg Lys Lys Asp Leu Gln Gln Ile Tyr Ala Glu Glu Arg Glu 
225                 230                 235                 240 


Asn Tyr Leu Gly Lys Leu Glu Arg Glu Ile Thr Arg Phe Phe Val Asp 
                245                 250                 255     


Arg Gly Phe Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Leu Glu Tyr 
            260                 265                 270         


Ile Glu Arg Phe Gly Ile Asp Asn Asp Thr Glu Leu Ser Lys Gln Ile 
        275                 280                 285             


Phe Arg Val Asp Lys Asn Phe Cys Leu Arg Pro Met Leu Ser Pro Asn 
    290                 295                 300                 


Leu Cys Asn Tyr Met Arg Lys Leu Asp Arg Ala Leu Pro Asp Pro Ile 
305                 310                 315                 320 


Lys Ile Phe Glu Ile Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys 
                325                 330                 335     


Glu His Leu Glu Glu Phe Thr Met Leu Asn Phe Cys Gln Met Gly Ser 
            340                 345                 350         


Gly Cys Thr Arg Glu Asn Leu Glu Ser Ile Ile Thr Asp Phe Leu Asn 
        355                 360                 365             


His Leu Gly Ile Asp Phe Lys Ile Val Gly Asp Ser Cys Met Val Tyr 
    370                 375                 380                 


Gly Asp Thr Leu Asp Val Met His Gly Asp Leu Glu Leu Ser Ser Ala 
385                 390                 395                 400 


Val Val Gly Pro Ile Pro Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro 
                405                 410                 415     


Trp Ile Gly Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Lys His 
            420                 425                 430         


Asp Phe Lys Asn Ile Lys Arg Ala Ala Arg Ser Glu Ser Tyr Tyr Asn 
        435                 440                 445             


Gly Ile Ser Thr Asn Leu 
    450                 


<210>  21
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  21
ggaaaggtct cgaccctgnn kaactatctg cgtaaactgg atcgtattc                   49


<210>  22
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
ggaaaggtct cagggtcggg gccagcatcg gacgcag                                37


<210>  23
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  23
ggaaaggtct ccatggttnn kttttgccaa atgggcagcg gctgcacc                    48


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
ggaaaggtct caccatggtg aattcttcca ggtgttcttt gc                          42


<210>  25
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  25
ggaaaggtct ccatggtgnn kggcgatacc ctggatatta tgcatgg                     47


<210>  26
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
ggaaaggtct caccatgcag ctatcgccca caatttcgaa gtc                         43


<210>  27
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  27
ggaaaggtct ccagcgcgnn kgtgggtccg gttagcctgg atcgtg                      46


<210>  28
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
ggaaaggtct cgcgctgctc agttccagat cgccatgc                               38


<210>  29
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  29
ggaaaggtct ctaaaccgnn kattggcgcg ggttttggcc tggaacg                     47


<210>  30
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
ggaaaggtct cggtttatca atgccccatt cacgatcc                               38


<210>  31
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
ggaaaggtct cgtagggcag tccattagta tcaactagag atgg                        44


<210>  32
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
ggaaaggtct cccctactgc ccatccttgg tttgaatcca atgc                        44


<210>  33
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
ggaaaggtct cggctggcag tccattagta tcaactagag atgg                        44


<210>  34
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
ggaaaggtct cccagcctgc ccatccttgg tttgaatcca atgc                        44


<210>  35
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
ttattacata tgcatcatca tcaccaccat c                                      31


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
ataataactc gagttagcca cgcg                                              24


<210>  37
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
gtgtatatcg gtggtcacta gctgggtggc catat                                  35


<210>  38
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
atatggccac ccagctagtg accaccgata tacac                                  35


