                         LISTAGEM DE SEQUNCIAS

<110>  FIOCRUZ FUNDAO OSWALDO CRUZ
 
<120>  "POLIPEPTDEO, CASSETE DE EXPRESSO, VETOR DE EXPRESSO, CLULA 
       HOSPEDEIRA, KIT PARA TRIAGEM IMUNOLGICA DE HCV E/OU DIAGNSTICO 
       DE HEPATITE C, COMPOSIO, USO DE PELO MENOS UM POLIPEPTDEO, E, 
       MTODOS PARA PRODUZIR UM POLIPEPTDEO, PARA TRIAGEM IMUNOLGICA 
       DE HCV E PARA O DIAGNSTICO DE HEPATITE C"

<130>  Caso 163

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  366
<212>  PRT
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NC e NS4 de HCV gentipo 3a

<400>  1

Met Ala Gly Ser Met Ser Thr Leu Pro Lys Pro Gln Arg Lys Thr Lys 
1               5                   10                  15      


Arg Asn Thr Ile Arg Arg Pro Pro Asp Val Lys Phe Pro Gly Gly Gly 
            20                  25                  30          


Gln Ile Val Gly Gly Val Tyr Val Leu Pro Arg Arg Gly Pro Arg Leu 
        35                  40                  45              


Gly Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Ser Gln Pro Arg Gly 
    50                  55                  60                  


Arg Arg Gln Pro Ile Pro Lys Ala Arg Arg Ser Glu Gly Arg Ser Trp 
65                  70                  75                  80  


Ala Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Cys Gly 
                85                  90                  95      


Trp Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Trp Gly 
            100                 105                 110         


Pro Asn Asp Pro Arg Arg Arg Ser Arg Asn Leu Lys Pro Ala Leu Val 
        115                 120                 125             


Pro Asp Lys Glu Val Leu Tyr Gln Gln Tyr Asp Glu Met Glu Glu Cys 
    130                 135                 140                 


Ser Gln Ala Ala Pro Tyr Ile Glu Gln Ala Gln Val Ile Ala His Gln 
145                 150                 155                 160 


Phe Lys Glu Lys Ser Leu Met Ala Phe Thr Ala Ser Val Thr Ser Pro 
                165                 170                 175     


Leu Thr Thr Asn Gln Thr Met Phe Phe Asn Ile Leu Gly Gly Trp Val 
            180                 185                 190         


Ala Thr His Leu Ala Gly Pro Gln Gly Ser Ser Ala Phe Val Val Ser 
        195                 200                 205             


Gly Leu Ala Gly Ala Ala Ile Gly Gly Ile Gly Leu Gly Lys Val Leu 
    210                 215                 220                 


Leu Asp Ile Leu Ala Gly Tyr Gly Ala Gly Val Ser Gly Ala Leu Val 
225                 230                 235                 240 


Ala Phe Lys Phe Ala Ser Arg Gly Asn His Val Ser Pro Thr His Tyr 
                245                 250                 255     


Val Pro Glu Ser Asp Ala Lys Ala Thr Cys Gln Thr His Arg Pro His 
            260                 265                 270         


Pro Asp Ala Glu Leu Val Asp Ala Asn Leu Leu Trp Gln Glu Met Gly 
        275                 280                 285             


Ser Asn Ile Thr Arg Val Glu Ser Glu Thr Lys Val Val Ile Leu Asp 
    290                 295                 300                 


Ser Phe Glu Pro Leu Arg Ala Glu Thr Asp Asp Thr Glu Leu Ser Val 
305                 310                 315                 320 


Ala Ala Glu Cys Phe Lys Lys Pro Pro Lys Tyr Pro Pro Ala Leu Pro 
                325                 330                 335     


Ile Trp Ala Arg Pro Asp Tyr Asn Pro Pro Leu Leu Asp Arg Trp Lys 
            340                 345                 350         


Ala Pro Asp Tyr Val Pro Pro Thr Val His Gly Ala Asn Ser 
        355                 360                 365 


<210>  2
<211>  1112
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NC e NS4 de HCV gentipo 3a

<400>  2
ccatggctgg atcctcaacg ctgccgaaac cgcaacgcaa aacgaaacgc aacacgattc       60

gccgtccgcc ggatgtgaag ttcccgggtg gtggtcagat tgtcggcggt gtttatgtcc      120

tgccgcgtcg cggtccgcgt ctgggtgtgc gcgcgacccg taaaacgagt gaacgttccc      180

agccgcgtgg tcgtcgccaa ccgattccga aggcgcgtcg cagtgaaggt cgttcctggg      240

cacagccggg ttatccgtgg ccgctgtacg gtaacgaagg ctgcggttgg gcaggttggc      300

tgctgtcacc gcgtggttca cgtccgtcgt ggggtccgaa cgatccgcgt cgccgttcgc      360

gcaatctgaa accggcgctg gtcccggata aggaagtgct gtatcagcaa tacgacgaaa      420

tggaagaatg tagccaggcg gccccgtaca ttgaacaggc gcaagttatc gcccatcaat      480

ttaaagaaaa gtctctgatg gcgttcaccg ccagcgtcac gtctccgctg accacgaacc      540

agaccatgtt tttcaatatt ctgggcggtt gggtggcaac gcacctggct ggtccgcaag      600

gcagctctgc atttgttgtt agtggtctgg ctggcgcagc tattggcggt atcggcctgg      660

gtaaagttct gctggatatc ctggcaggtt atggtgcagg tgttagcggt gcactggtcg      720

cttttaaatt cgcctcacgc ggtaaccatg tgtcgccgac ccactacgtt ccggaaagcg      780

acgcaaaggc tacctgccag acgcatcgtc cgcacccgga tgcagaactg gtggacgcta      840

acctgctgtg gcgccaggaa atgggcagca atattacccg tgttgaatct gaaacgaaag      900

tcgtgatcct ggatagtttt gaaccgctgc gtgccgaaac cgatgacacg gaactgtccg      960

tggcggccga atgtttcaaa aagccgccga aatatccgcc ggcactgccg atctgggcac     1020

gtccggatta caatccgccg ctgctggatc gttggaaagc accggactat gtgccgccga     1080

ccgttcatgg tgcgaattcc tgataaaagc tt                                   1112


<210>  3
<211>  261
<212>  PRT
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NC e NS4 de HCV gentipo 1b

<400>  3

Met Ala Gly Ser Ser Thr Asn Pro Lys Pro Gln Arg Lys Ile Lys Arg 
1               5                   10                  15      


Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe Pro Gly Gly Gly Gln 
            20                  25                  30          


Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg Gly Pro Arg Leu Gly 
        35                  40                  45              


Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Pro Gln Pro Arg Gly Arg 
    50                  55                  60                  


Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu Gly Arg Ala Trp Ala 
65                  70                  75                  80  


Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Leu Gly Trp 
                85                  90                  95      


Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Trp Gly Pro 
            100                 105                 110         


Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Arg Pro Ala Val Ile Pro 
        115                 120                 125             


Asp Arg Glu Val Leu Tyr Gln Glu Phe Asp Glu Met Glu Glu Cys Ala 
    130                 135                 140                 


Ser His Leu Pro Tyr Ile Glu Gln Gly Met Gln Leu Ala Glu Gln Phe 
145                 150                 155                 160 


Lys Gln Lys Ser Leu Met Ala Phe Thr Ala Ser Ile Thr Ser Pro Leu 
                165                 170                 175     


Thr Thr Gln Tyr Thr Leu Leu Phe Asn Ile Leu Gly Gly Trp Val Ala 
            180                 185                 190         


Ala Gln Leu Ala Pro Pro Ser Ala Ala Ser Ala Phe Val Gly Ala Gly 
        195                 200                 205             


Ile Ala Gly Ala Ala Val Gly Ser Ile Gly Leu Gly Lys Val Leu Val 
    210                 215                 220                 


Asp Ile Leu Ala Gly Tyr Gly Ala Gly Val Ala Gly Ala Leu Val Ala 
225                 230                 235                 240 


Phe Lys Phe Ala Ser Arg Gly Asn His Val Ser Pro Thr His Tyr Val 
                245                 250                 255     


Pro Glu Ser Asp Ala 
            260              


<210>  4
<211>  798
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NC e NS4 de HCV gentipo 1b

<400>  4
ccatggctgg atcctcaacc aatccgaagc cgcaacgtaa aatcaagcgt aataccaatc       60

gtcgtccgca agatgtcaag tttccgggtg gcggtcaaat cgtgggcggt gtttatctgc      120

tgccgcgtcg cggtccgcgt ctgggtgtgc gcgcgacccg taaaacgagt gaacgtccgc      180

agccgcgtgg tcgtcgccaa ccgattccga aggcacgtca gccggaaggt cgtgcatggg      240

ctcaaccggg ctatccgtgg ccgctgtacg gtaacgaagg cctgggttgg gcaggttggc      300

tgctgtcacc gcgtggtagc cgtccgtctt ggggtccgac cgatccgcgt cgccgttcgc      360

gtaatctgcg tccggccgtt attccggatc gtgaagtcct gtatcaggaa tttgacgaaa      420

tggaagaatg cgccagccat ctgccgtaca tcgaacaggg catgcaactg gcagaacagt      480

ttaaacaaaa gtctctgatg gcgttcaccg ccagtattac gtccccgctg accacgcagt      540

acaccctgct gtttaacatc ctgggcggtt gggttgcagc acaactggca ccgccgtcag      600

cagcttcggc attcgttggt gcaggtattg ctggtgcagc agtcggctct atcggtctgg      660

gtaaagtcct ggtggatatt ctggcaggtt atggtgcagg tgtcgcaggt gccctggtgg      720

catttaaatt cgctagccgc ggtaaccatg tgtctccgac ccactacgtt ccggaaagcg      780

acgcataaaa gcttcatg                                                    798


<210>  5
<211>  480
<212>  PRT
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NS3 e NS5 de HCV gentipo 1b

<400>  5

Met Ala Gly Ser Gly Pro Lys Gly Pro Ile Thr Gln Met Tyr Thr Asn 
1               5                   10                  15      


Val Asp Gln Asp Leu Val Gly Trp Pro Ala Pro Pro Gly Ala Arg Ser 
            20                  25                  30          


Met Thr Pro Cys Thr Cys Gly Ser Ser Asp Leu Tyr Leu Val Thr Arg 
        35                  40                  45              


His Ala Asp Val Ile Pro Val Arg Arg Arg Gly Asp Ser Arg Gly Ser 
    50                  55                  60                  


Leu Leu Ser Pro Arg Pro Ile Ser Tyr Leu Lys Gly Ser Ser Gly Gly 
65                  70                  75                  80  


Pro Leu Leu Cys Pro Ser Gly His Val Val Gly Ile Phe Arg Ala Ala 
                85                  90                  95      


Val Cys Thr Arg Gly Val Ala Lys Ala Val Asp Phe Val Pro Val Glu 
            100                 105                 110         


Ser Met Glu Thr Thr Met Arg Ser Pro Val Phe Thr Asp Asn Ser Thr 
        115                 120                 125             


Pro Pro Ala Val Pro Gln Ser Phe Gln Val Ala His Leu His Ala Pro 
    130                 135                 140                 


Thr Gly Ser Gly Lys Ser Thr Lys Val Pro Ala Ala Tyr Ala Ala Gln 
145                 150                 155                 160 


Gly Tyr Lys Val Leu Val Leu Asn Pro Ser Val Ala Ala Thr Leu Gly 
                165                 170                 175     


Phe Gly Ala Tyr Met Ser Lys Ala His Gly Val Asp Pro Asn Ile Arg 
            180                 185                 190         


Thr Gly Val Arg Thr Ile Thr Thr Gly Ala Pro Ile Thr Tyr Ser Thr 
        195                 200                 205             


Tyr Gly Lys Phe Leu Ala Asp Gly Gly Cys Ser Gly Gly Ala Tyr Asp 
    210                 215                 220                 


Ile Ile Ile Cys Asp Glu Cys His Ser Thr Asp Ser Thr Ser Ile Leu 
225                 230                 235                 240 


Gly Ile Gly Thr Val Leu Asp Gln Ala Glu Thr Ala Gly Ala Arg Leu 
                245                 250                 255     


Val Val Leu Ala Thr Ala Thr Pro Pro Gly Ser Val Thr Val Pro His 
            260                 265                 270         


Pro Asn Ile Glu Glu Val Ala Leu Ser Asn Thr Gly Glu Ile Pro Phe 
        275                 280                 285             


Tyr Gly Lys Ala Ile Pro Leu Glu Ala Ile Lys Gly Gly Arg His Leu 
    290                 295                 300                 


Ile Phe Cys His Ser Lys Lys Lys Cys Asp Glu Leu Ala Ala Lys Leu 
305                 310                 315                 320 


Ser Ala Leu Gly Val Asn Ala Val Ala Tyr Tyr Arg Gly Leu Asp Val 
                325                 330                 335     


Ser Ile Ile Pro Thr Ser Gly Asp Val Val Val Val Ala Thr Asp Ala 
            340                 345                 350         


Leu Met Thr Gly Tyr Thr Gly Asp Phe Asp Ser Val Ile Asp Cys Asn 
        355                 360                 365             


Thr Cys Val Thr Ala Asn Ser Lys Ala Thr Cys Thr Thr His His Gly 
    370                 375                 380                 


Ala Pro Asp Thr Asp Leu Ile Glu Ala Asn Leu Leu Trp Arg Gln Glu 
385                 390                 395                 400 


Met Gly Gly Asn Ile Thr Arg Val Glu Ser Glu Asn Lys Ile Val Ile 
                405                 410                 415     


Leu Asp Ser Phe Glu Pro Leu Arg Ala Glu Glu Asp Glu Arg Glu Val 
            420                 425                 430         


Ser Val Ala Ala Glu Ile Leu Arg Lys Thr Arg Lys Phe Pro Ala Ala 
        435                 440                 445             


Met Pro Val Trp Ala Arg Pro Asp Tyr Asn Pro Pro Leu Leu Glu Ser 
    450                 455                 460                 


Trp Lys Asn Pro Asp Tyr Val Pro Pro Val Val His Gly Ala Asn Ser 
465                 470                 475                 480 
        

<210>  6
<211>  1355
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NS3 e NS5 de HCV gentipo 1b

<400>  6
ccatggctgg atccggtccg aagggtccga ttacgcaaat gtatacgaat gtcgatcaag       60

acctggttgg ctggccggca ccgccgggcg ctcgctctat gaccccgtgc acgtgtggca      120

gctctgatct gtatctggtg acccgtcatg cagacgtcat cccggtgcgt cgccgtggtg      180

atagccgtgg ttctctgctg agtccgcgtc cgatttcata cctgaagggc agttccggcg      240

gtccgctgct gtgcccgtcg ggtcacgtgg ttggtatctt tcgtgcagca gtgtgtaccc      300

gtggtgtggc aaaagctgtt gacttcgtcc cggtggaatc tatggaaacc acgatgcgta      360

gtccggtttt taccgataac tccacgccgc cggcagtccc gcagagtttc caagtggcac      420

atctgcacgc accgaccggc tccggcaagt caacgaaagt cccggcagct tatgcggccc      480

agggttacaa ggttctggtc ctgaacccgt cagttgcagc taccctgggc tttggtgcat      540

atatgtcgaa agctcatggc gttgatccga atattcgcac cggcgttcgt acgatcacca      600

cgggtgcccc gattacctat agcacgtacg gtaaattcct ggcagatggc ggttgctctg      660

gcggtgctta cgatattatc atttgcgacg aatgtcatag caccgactca acgtcgatcc      720

tgggcattgg taccgtgctg gatcaagcag aaacggcagg tgcacgcctg gtcgtgctgg      780

caaccgctac gccgccgggt tcagtgaccg ttccgcaccc gaacatcgaa gaagttgcac      840

tgtctaatac cggcgaaatt ccgttttatg gcaaggcgat cccgctggaa gccattaaag      900

gcggtcgcca tctgatcttc tgccacagta aaaagaaatg tgacgaactg gcggccaaac      960

tgtccgcact gggtgttaac gcagtcgcat attaccgtgg tctggatgtg tcgatcattc     1020

cgaccagcgg cgatgttgtc gtggttgcaa ccgacgctct gatgaccggc tatacgggtg     1080

attttgacag cgttattgat tgcaatacct gtgtcacggc gaattccaag gctacctgta     1140

ccacgcatca cggcgcaccg gatacggacc tgattgaagc taatctgctg tggcgccagg     1200

aaatgggcgg taacatcacc cgtgtcgaaa gtgaaaacaa gatcgtgatc ctggattcct     1260

ttgaaccgct gcgcgcggaa gaagacgaac gtgaagttag tgtcgcagct gaaatcctgc     1320

gcaaaacgcg taagttcccg gcagcaatgc cggtg                                1355


<210>  7
<211>  514
<212>  PRT
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NS3 e NS4 de HCV gentipo 1b

<400>  7

Met Ala Gly Ser Gly Pro Lys Gly Pro Ile Thr Gln Met Tyr Thr Asn 
1               5                   10                  15      


Val Asp Gln Asp Leu Val Gly Trp Pro Ala Pro Pro Gly Ala Arg Ser 
            20                  25                  30          


Met Thr Pro Cys Thr Cys Gly Ser Ser Asp Leu Tyr Leu Val Thr Arg 
        35                  40                  45              


His Ala Asp Val Ile Pro Val Arg Arg Arg Gly Asp Ser Arg Gly Ser 
    50                  55                  60                  


Leu Leu Ser Pro Arg Pro Ile Ser Tyr Leu Lys Gly Ser Ser Gly Gly 
65                  70                  75                  80  


Pro Leu Leu Cys Pro Ser Gly His Val Val Gly Ile Phe Arg Ala Ala 
                85                  90                  95      


Val Cys Thr Arg Gly Val Ala Lys Ala Val Asp Phe Val Pro Val Glu 
            100                 105                 110         


Ser Met Glu Thr Thr Met Arg Ser Pro Val Phe Thr Asp Asn Ser Thr 
        115                 120                 125             


Pro Pro Ala Val Pro Gln Ser Phe Gln Val Ala His Leu His Ala Pro 
    130                 135                 140                 


Thr Gly Ser Gly Lys Ser Thr Lys Val Pro Ala Ala Tyr Ala Ala Gln 
145                 150                 155                 160 


Gly Tyr Lys Val Leu Val Leu Asn Pro Ser Val Ala Ala Thr Leu Gly 
                165                 170                 175     


Phe Gly Ala Tyr Met Ser Lys Ala His Gly Val Asp Pro Asn Ile Arg 
            180                 185                 190         


Thr Gly Val Arg Thr Ile Thr Thr Gly Ala Pro Ile Thr Tyr Ser Thr 
        195                 200                 205             


Tyr Gly Lys Phe Leu Ala Asp Gly Gly Cys Ser Gly Gly Ala Tyr Asp 
    210                 215                 220                 


Ile Ile Ile Cys Asp Glu Cys His Ser Thr Asp Ser Thr Ser Ile Leu 
225                 230                 235                 240 


Gly Ile Gly Thr Val Leu Asp Gln Ala Glu Thr Ala Gly Ala Arg Leu 
                245                 250                 255     


Val Val Leu Ala Thr Ala Thr Pro Pro Gly Ser Val Thr Val Pro His 
            260                 265                 270         


Pro Asn Ile Glu Glu Val Ala Leu Ser Asn Thr Gly Glu Ile Pro Phe 
        275                 280                 285             


Tyr Gly Lys Ala Ile Pro Leu Glu Ala Ile Lys Gly Gly Arg His Leu 
    290                 295                 300                 


Ile Phe Cys His Ser Lys Lys Lys Cys Asp Glu Leu Ala Ala Lys Leu 
305                 310                 315                 320 


Ser Ala Leu Gly Val Asn Ala Val Ala Tyr Tyr Arg Gly Leu Asp Val 
                325                 330                 335     


Ser Ile Ile Pro Thr Ser Gly Asp Val Val Val Val Ala Thr Asp Ala 
            340                 345                 350         


Leu Met Thr Gly Tyr Thr Gly Asp Phe Asp Ser Val Ile Asp Cys Asn 
        355                 360                 365             


Thr Cys Val Thr Ala Asn Ser Arg Pro Ala Val Ile Pro Asp Arg Glu 
    370                 375                 380                 


Val Leu Tyr Gln Glu Phe Asp Glu Met Glu Glu Cys Ala Ser His Leu 
385                 390                 395                 400 


Pro Tyr Ile Glu Gln Gly Met Gln Leu Ala Glu Gln Phe Lys Gln Lys 
                405                 410                 415     


Ser Leu Met Ala Phe Thr Ala Ser Ile Thr Ser Pro Leu Thr Thr Gln 
            420                 425                 430         


Tyr Thr Leu Leu Phe Asn Ile Leu Gly Gly Trp Val Ala Ala Gln Leu 
        435                 440                 445             


Ala Pro Pro Ser Ala Ala Ser Ala Phe Val Gly Ala Gly Ile Ala Gly 
    450                 455                 460                 


Ala Ala Val Gly Ser Ile Gly Leu Gly Lys Val Leu Val Asp Ile Leu 
465                 470                 475                 480 


Ala Gly Tyr Gly Ala Gly Val Ala Gly Ala Leu Val Ala Phe Lys Phe 
                485                 490                 495     


Ala Ser Arg Gly Asn His Val Ser Pro Thr His Tyr Val Pro Glu Ser 
            500                 505                 510         


Asp Ala    


<210>  8
<211>  1553
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NS3 e NS4 de HCV gentipo 1b

<400>  8
ccatggctgg atccggtccg aagggtccga ttacgcaaat gtatacgaat gtcgatcaag       60

acctggttgg ctggccggca ccgccgggcg ctcgctctat gaccccgtgc acgtgtggca      120

gctctgatct gtatctggtg acccgtcatg cagacgtcat cccggtgcgt cgccgtggtg      180

atagccgtgg ttctctgctg agtccgcgtc cgatttcata cctgaagggc agttccggcg      240

gtccgctgct gtgcccgtcg ggtcacgtgg ttggtatctt tcgtgcagca gtgtgtaccc      300

gtggtgtggc aaaagctgtt gacttcgtcc cggtggaatc tatggaaacc acgatgcgta      360

gtccggtttt taccgataac tccacgccgc cggcagtccc gcagagtttc caagtggcac      420

atctgcacgc accgaccggc tccggcaagt caacgaaagt cccggcagct tatgcggccc      480

agggttacaa ggttctggtc ctgaacccgt cagttgcagc taccctgggc tttggtgcat      540

atatgtcgaa agctcatggc gttgatccga atattcgcac cggcgttcgt acgatcacca      600

cgggtgcccc gattacctat agcacgtacg gtaaattcct ggcagatggc ggttgctctg      660

gcggtgctta cgatattatc atttgcgacg aatgtcatag caccgactca acgtcgatcc      720

tgggcattgg taccgtgctg gatcaagcag aaacggcagg tgcacgcctg gtcgtgctgg      780

caaccgctac gccgccgggt tcagtgaccg ttccgcaccc gaacatcgaa gaagttgcac      840

tgtctaatac cggcgaaatt ccgttttatg gcaaggcgat cccgctggaa gccattaaag      900

gcggtcgcca tctgatcttc tgccacagta aaaagaaatg tgacgaactg gcggccaaac      960

tgtccgcact gggtgttaac gcagtcgcat attaccgtgg tctggatgtg tcgatcattc     1020

cgaccagcgg cgatgttgtc gtggttgcaa ccgacgctct gatgaccggc tatacgggtg     1080

attttgacag cgttattgat tgcaatacct gtgtcacggc gaattcccgt ccggccgtta     1140

ttccggatcg tgaagtcctg tatcaggaat ttgacgaaat ggaagaatgc gccagccatc     1200

tgccgtacat cgaacagggc atgcaactgg cagaacagtt taaacaaaag tctctgatgg     1260

cgttcaccgc cagtattacg tccccgctga ccacgcagta caccctgctg tttaacatcc     1320

tgggcggttg ggttgcagca caactggcac cgccgtcagc agcttcggca ttcgttggtg     1380

caggtattgc tggtgcagca gtcggctcta tcggtctggg taaagtcctg gtggatattc     1440

tggcaggtta tggtgcaggt gtcgcaggtg ccctggtggc atttaaattc gctagccgcg     1500

gtaaccatgt gtctccgacc cactacgttc cggaaagcga cgcataaaag ctt            1553


<210>  9
<211>  298
<212>  PRT
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NC e NS5 de HCV gentipo 1b

<400>  9

Met Ala Gly Ser Ser Thr Asn Pro Lys Pro Gln Arg Lys Ile Lys Arg 
1               5                   10                  15      


Asn Thr Asn Arg Arg Pro Gln Asp Val Lys Phe Pro Gly Gly Gly Gln 
            20                  25                  30          


Ile Val Gly Gly Val Tyr Leu Leu Pro Arg Arg Gly Pro Arg Leu Gly 
        35                  40                  45              


Val Arg Ala Thr Arg Lys Thr Ser Glu Arg Pro Gln Pro Arg Gly Arg 
    50                  55                  60                  


Arg Gln Pro Ile Pro Lys Ala Arg Gln Pro Glu Gly Arg Ala Trp Ala 
65                  70                  75                  80  


Gln Pro Gly Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Leu Gly Trp 
                85                  90                  95      


Ala Gly Trp Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Trp Gly Pro 
            100                 105                 110         


Thr Asp Pro Arg Arg Arg Ser Arg Asn Leu Gly Lys Val Ile Asp Thr 
        115                 120                 125             


Leu Thr Cys Gly Phe Ala Asp Leu Met Gly Tyr Ile Pro Leu Val Gly 
    130                 135                 140                 


Ala Pro Leu Gly Gly Ala Ala Arg Ala Leu Ala His Gly Val Arg Val 
145                 150                 155                 160 


Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gly Cys Ser 
                165                 170                 175     


Phe Ser Ile Phe Leu Leu Ala Leu Leu Ser Cys Leu Thr Ile Pro Ala 
            180                 185                 190         


Ser Ala Asn Ser Lys Ala Thr Cys Thr Thr His His Gly Ala Pro Asp 
        195                 200                 205             


Thr Asp Leu Ile Glu Ala Asn Leu Leu Trp Arg Gln Glu Met Gly Gly 
    210                 215                 220                 


Asn Ile Thr Arg Val Glu Ser Glu Asn Lys Ile Val Ile Leu Asp Ser 
225                 230                 235                 240 


Phe Glu Pro Leu Arg Ala Glu Glu Asp Glu Arg Glu Val Ser Val Ala 
                245                 250                 255     


Ala Glu Ile Leu Arg Lys Thr Arg Lys Phe Pro Ala Ala Met Pro Val 
            260                 265                 270         


Trp Ala Arg Pro Asp Tyr Asn Pro Pro Leu Leu Glu Ser Trp Lys Asn 
        275                 280                 285             


Pro Asp Tyr Val Pro Pro Val Val His Gly 
    290                 295 


<210>  10
<211>  902
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Poliantgeno composto das regies NC e NS5 de HCV gentipo 1b

<400>  10
ccatggctgg atccagcacg aatccgaagc cgcaacgcaa aatcaaacgc aatacgaacc       60

gccgtccgca agatgtcaag tttccgggtg gtggtcaaat cgtcggcggt gtgtatctgc      120

tgccgcgtcg cggtccgcgt ctgggtgtgc gcgcaacccg taaaacgtcc gaacgtccgc      180

agccgcgtgg tcgtcgccaa ccgattccga aggctcgtca gccggaaggt cgtgcatggg      240

ctcaaccggg ctatccgtgg ccgctgtacg gtaacgaagg tctgggttgg gcaggttggc      300

tgctgagccc gcgtggcagt cgtccgtcct ggggtccgac cgatccgcgt cgccgttctc      360

gtaatctggg taaagttatt gataccctga cgtgcggctt tgcggacctg atgggttata      420

tcccgctggt cggcgcaccg ctgggcggtg cagcacgtgc gctggcgcat ggcgtgcgtg      480

ttctggaaga cggtgttaac tacgccaccg gcaatctgcc gggttgcagc ttttctattt      540

tcctgctggc actgctgtca tgtctgacga tcccggcttc ggcgaattcc aaggctacct      600

gtaccacgca tcacggcgca ccggatacgg acctgattga agctaatctg ctgtggcgcc      660

aggaaatggg cggtaacatc acccgtgtcg aaagtgaaaa caagatcgtg atcctggatt      720

cctttgaacc gctgcgcgcg gaagaagacg aacgtgaagt tagtgtcgca gctgaaatcc      780

tgcgcaaaac gcgtaagttc ccggcagcaa tgccggtgtg ggcacgtccg gattataacc      840

cgccgctgct ggaatcctgg aaaaatccgg actacgttcc gccggtggtt cacggtaagc      900

tt                                                                     902


<210>  11
<211>  26
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Iniciador direto

<400>  11
ccatggctgg atcctcaacc aatccg                                            26


<210>  12
<211>  39
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Iniciador reverso

<400>  12
catgaagctt ttatgcgtag ctttccggaa cgtagtggg                              39


<210>  13
<211>  33
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Iniciador direto

<400>  13
gcgaattcca aggctacctg taccacgcat cac                                    33


<210>  14
<211>  35
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Iniciador reverso

<400>  14
catgaagctt ttaaccgtga accaccggcg gaacg                                  35


<210>  15
<211>  31
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Iniciador direto

<400>  15
gcgaattccg tccggccgtt attccggatc g                                      31


<210>  16
<211>  39
<212>  DNA
<213>  Sequncia Artificial

<220>
<223>  Iniciador reverso

<400>  16
catgaagctt ttatgcgtcg ctttccggaa cgtagtggg                              39
