                         SEQUENCE LISTING

<110>  THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE
       GILL, Ryan T.
       BASSALO, Marcelo C.
 
<120>  COMPOSITIONS AND METHODS FOR GENERATING MODIFIED MICROORGANISMS 
       FOR THE PRODUCTION OF AMINO ACIDS AND RELATED MOLECULES

<130>  101877.639065 CU4347B-PCT1

<150>  62/747,479
<151>  2018-10-18

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  311
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derived from Escherichia coli, referred to as lysR_S36R

<400>  1

Met Ala Ala Val Asn Leu Arg His Ile Glu Ile Phe His Ala Val Met 
1               5                   10                  15      


Thr Ala Gly Ser Leu Thr Glu Ala Ala His Leu Leu His Thr Ser Gln 
            20                  25                  30          


Pro Thr Val Arg Arg Glu Leu Ala Arg Phe Glu Lys Val Ile Gly Leu 
        35                  40                  45              


Lys Leu Phe Glu Arg Val Arg Gly Arg Leu His Pro Thr Val Gln Gly 
    50                  55                  60                  


Leu Arg Leu Phe Glu Glu Val Gln Arg Ser Trp Tyr Gly Leu Asp Arg 
65                  70                  75                  80  


Ile Val Ser Ala Ala Glu Ser Leu Arg Glu Phe Arg Gln Gly Glu Leu 
                85                  90                  95      


Ser Ile Ala Cys Leu Pro Val Phe Ser Gln Ser Phe Leu Pro Gln Leu 
            100                 105                 110         


Leu Gln Pro Phe Leu Ala Arg Tyr Pro Asp Val Ser Leu Asn Ile Val 
        115                 120                 125             


Pro Gln Glu Ser Pro Leu Leu Glu Glu Trp Leu Ser Ala Gln Arg His 
    130                 135                 140                 


Asp Leu Gly Leu Thr Glu Thr Leu His Thr Pro Ala Gly Thr Glu Arg 
145                 150                 155                 160 


Thr Glu Leu Leu Ser Leu Asp Glu Val Cys Val Leu Pro Pro Gly His 
                165                 170                 175     


Pro Leu Ala Val Lys Lys Val Leu Thr Pro Asp Asp Phe Gln Gly Glu 
            180                 185                 190         


Asn Tyr Ile Ser Leu Ser Arg Thr Asp Ser Tyr Arg Gln Leu Leu Asp 
        195                 200                 205             


Gln Leu Phe Thr Glu His Gln Val Lys Arg Arg Met Ile Val Glu Thr 
    210                 215                 220                 


His Ser Ala Ala Ser Val Cys Ala Met Val Arg Ala Gly Val Gly Ile 
225                 230                 235                 240 


Ser Val Val Asn Pro Leu Thr Ala Leu Asp Tyr Ala Ala Ser Gly Leu 
                245                 250                 255     


Val Val Arg Arg Phe Ser Ile Ala Val Pro Phe Thr Val Ser Leu Ile 
            260                 265                 270         


Arg Pro Leu His Arg Pro Ser Ser Ala Leu Val Gln Ala Phe Ser Gly 
        275                 280                 285             


His Leu Gln Ala Gly Leu Pro Lys Leu Val Thr Ser Leu Asp Ala Ile 
    290                 295                 300                 


Leu Ser Ser Ala Thr Thr Ala 
305                 310     


<210>  2
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derived from Escherichia coli, referred to as lysP_T33F

<400>  2

Met Val Ser Glu Thr Lys Thr Thr Glu Ala Pro Gly Leu Arg Arg Glu 
1               5                   10                  15      


Leu Lys Ala Arg His Leu Phe Met Ile Ala Ile Gly Gly Ser Ile Gly 
            20                  25                  30          


Thr Gly Leu Phe Val Ala Ser Gly Ala Thr Ile Ser Gln Ala Gly Pro 
        35                  40                  45              


Gly Gly Ala Leu Leu Ser Tyr Met Leu Ile Gly Leu Met Val Tyr Phe 
    50                  55                  60                  


Leu Met Thr Ser Leu Gly Glu Leu Ala Ala Tyr Met Pro Val Ser Gly 
65                  70                  75                  80  


Ser Phe Ala Thr Tyr Gly Gln Asn Tyr Val Glu Glu Gly Phe Gly Phe 
                85                  90                  95      


Ala Leu Gly Trp Asn Tyr Trp Tyr Asn Trp Ala Val Thr Ile Ala Val 
            100                 105                 110         


Asp Leu Val Ala Ala Gln Leu Val Met Ser Trp Trp Phe Pro Asp Thr 
        115                 120                 125             


Pro Gly Trp Ile Trp Ser Ala Leu Phe Leu Gly Val Ile Phe Leu Leu 
    130                 135                 140                 


Asn Tyr Ile Ser Val Arg Gly Phe Gly Glu Ala Glu Tyr Trp Phe Ser 
145                 150                 155                 160 


Leu Ile Lys Val Thr Thr Val Ile Val Phe Ile Ile Val Gly Val Leu 
                165                 170                 175     


Met Ile Ile Gly Ile Phe Lys Gly Ala Gln Pro Ala Gly Trp Ser Asn 
            180                 185                 190         


Trp Thr Ile Gly Glu Ala Pro Phe Ala Gly Gly Phe Ala Ala Met Ile 
        195                 200                 205             


Gly Val Ala Met Ile Val Gly Phe Ser Phe Gln Gly Thr Glu Leu Ile 
    210                 215                 220                 


Gly Ile Ala Ala Gly Glu Ser Glu Asp Pro Ala Lys Asn Ile Pro Arg 
225                 230                 235                 240 


Ala Val Arg Gln Val Phe Trp Arg Ile Leu Leu Phe Tyr Val Phe Ala 
                245                 250                 255     


Ile Leu Ile Ile Ser Leu Ile Ile Pro Tyr Thr Asp Pro Ser Leu Leu 
            260                 265                 270         


Arg Asn Asp Val Lys Asp Ile Ser Val Ser Pro Phe Thr Leu Val Phe 
        275                 280                 285             


Gln His Ala Gly Leu Leu Ser Ala Ala Ala Val Met Asn Ala Val Ile 
    290                 295                 300                 


Leu Thr Ala Val Leu Ser Ala Gly Asn Ser Gly Met Tyr Ala Ser Thr 
305                 310                 315                 320 


Arg Met Leu Tyr Thr Leu Ala Cys Asp Gly Lys Ala Pro Arg Ile Phe 
                325                 330                 335     


Ala Lys Leu Ser Arg Gly Gly Val Pro Arg Asn Ala Leu Tyr Ala Thr 
            340                 345                 350         


Thr Val Ile Ala Gly Leu Cys Phe Leu Thr Ser Met Phe Gly Asn Gln 
        355                 360                 365             


Thr Val Tyr Leu Trp Leu Leu Asn Thr Ser Gly Met Thr Gly Phe Ile 
    370                 375                 380                 


Ala Trp Leu Gly Ile Ala Ile Ser His Tyr Arg Phe Arg Arg Gly Tyr 
385                 390                 395                 400 


Val Leu Gln Gly His Asp Ile Asn Asp Leu Pro Tyr Arg Ser Gly Phe 
                405                 410                 415     


Phe Pro Leu Gly Pro Ile Phe Ala Phe Ile Leu Cys Leu Ile Ile Thr 
            420                 425                 430         


Leu Gly Gln Asn Tyr Glu Ala Phe Leu Lys Asp Thr Ile Asp Trp Gly 
        435                 440                 445             


Gly Val Ala Ala Thr Tyr Ile Gly Ile Pro Leu Phe Leu Ile Ile Trp 
    450                 455                 460                 


Phe Gly Tyr Lys Leu Ile Lys Gly Thr His Phe Val Arg Tyr Ser Glu 
465                 470                 475                 480 


Met Lys Phe Pro Gln Asn Asp Lys Lys 
                485                 


<210>  3
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derived from Escherichia coli, referred to as lysP_Q219I

<400>  3

Met Val Ser Glu Thr Lys Thr Thr Glu Ala Pro Gly Leu Arg Arg Glu 
1               5                   10                  15      


Leu Lys Ala Arg His Leu Thr Met Ile Ala Ile Gly Gly Ser Ile Gly 
            20                  25                  30          


Thr Gly Leu Phe Val Ala Ser Gly Ala Thr Ile Ser Gln Ala Gly Pro 
        35                  40                  45              


Gly Gly Ala Leu Leu Ser Tyr Met Leu Ile Gly Leu Met Val Tyr Phe 
    50                  55                  60                  


Leu Met Thr Ser Leu Gly Glu Leu Ala Ala Tyr Met Pro Val Ser Gly 
65                  70                  75                  80  


Ser Phe Ala Thr Tyr Gly Gln Asn Tyr Val Glu Glu Gly Phe Gly Phe 
                85                  90                  95      


Ala Leu Gly Trp Asn Tyr Trp Tyr Asn Trp Ala Val Thr Ile Ala Val 
            100                 105                 110         


Asp Leu Val Ala Ala Gln Leu Val Met Ser Trp Trp Phe Pro Asp Thr 
        115                 120                 125             


Pro Gly Trp Ile Trp Ser Ala Leu Phe Leu Gly Val Ile Phe Leu Leu 
    130                 135                 140                 


Asn Tyr Ile Ser Val Arg Gly Phe Gly Glu Ala Glu Tyr Trp Phe Ser 
145                 150                 155                 160 


Leu Ile Lys Val Thr Thr Val Ile Val Phe Ile Ile Val Gly Val Leu 
                165                 170                 175     


Met Ile Ile Gly Ile Phe Lys Gly Ala Gln Pro Ala Gly Trp Ser Asn 
            180                 185                 190         


Trp Thr Ile Gly Glu Ala Pro Phe Ala Gly Gly Phe Ala Ala Met Ile 
        195                 200                 205             


Gly Val Ala Met Ile Val Gly Phe Ser Phe Ile Gly Thr Glu Leu Ile 
    210                 215                 220                 


Gly Ile Ala Ala Gly Glu Ser Glu Asp Pro Ala Lys Asn Ile Pro Arg 
225                 230                 235                 240 


Ala Val Arg Gln Val Phe Trp Arg Ile Leu Leu Phe Tyr Val Phe Ala 
                245                 250                 255     


Ile Leu Ile Ile Ser Leu Ile Ile Pro Tyr Thr Asp Pro Ser Leu Leu 
            260                 265                 270         


Arg Asn Asp Val Lys Asp Ile Ser Val Ser Pro Phe Thr Leu Val Phe 
        275                 280                 285             


Gln His Ala Gly Leu Leu Ser Ala Ala Ala Val Met Asn Ala Val Ile 
    290                 295                 300                 


Leu Thr Ala Val Leu Ser Ala Gly Asn Ser Gly Met Tyr Ala Ser Thr 
305                 310                 315                 320 


Arg Met Leu Tyr Thr Leu Ala Cys Asp Gly Lys Ala Pro Arg Ile Phe 
                325                 330                 335     


Ala Lys Leu Ser Arg Gly Gly Val Pro Arg Asn Ala Leu Tyr Ala Thr 
            340                 345                 350         


Thr Val Ile Ala Gly Leu Cys Phe Leu Thr Ser Met Phe Gly Asn Gln 
        355                 360                 365             


Thr Val Tyr Leu Trp Leu Leu Asn Thr Ser Gly Met Thr Gly Phe Ile 
    370                 375                 380                 


Ala Trp Leu Gly Ile Ala Ile Ser His Tyr Arg Phe Arg Arg Gly Tyr 
385                 390                 395                 400 


Val Leu Gln Gly His Asp Ile Asn Asp Leu Pro Tyr Arg Ser Gly Phe 
                405                 410                 415     


Phe Pro Leu Gly Pro Ile Phe Ala Phe Ile Leu Cys Leu Ile Ile Thr 
            420                 425                 430         


Leu Gly Gln Asn Tyr Glu Ala Phe Leu Lys Asp Thr Ile Asp Trp Gly 
        435                 440                 445             


Gly Val Ala Ala Thr Tyr Ile Gly Ile Pro Leu Phe Leu Ile Ile Trp 
    450                 455                 460                 


Phe Gly Tyr Lys Leu Ile Lys Gly Thr His Phe Val Arg Tyr Ser Glu 
465                 470                 475                 480 


Met Lys Phe Pro Gln Asn Asp Lys Lys 
                485                 


<210>  4
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derived from Escherichia coli, referred to as dapF_G210D

<400>  4

Met Gln Phe Ser Lys Met His Gly Leu Gly Asn Asp Phe Met Val Val 
1               5                   10                  15      


Asp Ala Val Thr Gln Asn Val Phe Phe Ser Pro Glu Leu Ile Arg Arg 
            20                  25                  30          


Leu Ala Asp Arg His Leu Gly Val Gly Phe Asp Gln Leu Leu Val Val 
        35                  40                  45              


Glu Pro Pro Tyr Asp Pro Glu Leu Asp Phe His Tyr Arg Ile Phe Asn 
    50                  55                  60                  


Ala Asp Gly Ser Glu Val Ala Gln Cys Gly Asn Gly Ala Arg Cys Phe 
65                  70                  75                  80  


Ala Arg Phe Val Arg Leu Lys Gly Leu Thr Asn Lys Arg Asp Ile Arg 
                85                  90                  95      


Val Ser Thr Ala Asn Gly Arg Met Val Leu Thr Val Thr Asp Asp Asp 
            100                 105                 110         


Leu Val Arg Val Asn Met Gly Glu Pro Asn Phe Glu Pro Ser Ala Val 
        115                 120                 125             


Pro Phe Arg Ala Asn Lys Ala Glu Lys Thr Tyr Ile Met Arg Ala Ala 
    130                 135                 140                 


Glu Gln Thr Ile Leu Cys Gly Val Val Ser Met Gly Asn Pro His Cys 
145                 150                 155                 160 


Val Ile Gln Val Asp Asp Val Asp Thr Ala Ala Val Glu Thr Leu Gly 
                165                 170                 175     


Pro Val Leu Glu Ser His Glu Arg Phe Pro Glu Arg Ala Asn Ile Gly 
            180                 185                 190         


Phe Met Gln Val Val Lys Arg Glu His Ile Arg Leu Arg Val Tyr Glu 
        195                 200                 205             


Arg Asp Ala Gly Glu Thr Gln Ala Cys Gly Ser Gly Ala Cys Ala Ala 
    210                 215                 220                 


Val Ala Val Gly Ile Gln Gln Gly Leu Leu Ala Glu Glu Val Arg Val 
225                 230                 235                 240 


Glu Leu Pro Gly Gly Arg Leu Asp Ile Ala Trp Lys Gly Pro Gly His 
                245                 250                 255     


Pro Leu Tyr Met Thr Gly Pro Ala Val His Val Tyr Asp Gly Phe Ile 
            260                 265                 270         


His Leu 
        


<210>  5
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derived from Escherichia coli, referred to as dapF_M260Y

<400>  5

Met Gln Phe Ser Lys Met His Gly Leu Gly Asn Asp Phe Met Val Val 
1               5                   10                  15      


Asp Ala Val Thr Gln Asn Val Phe Phe Ser Pro Glu Leu Ile Arg Arg 
            20                  25                  30          


Leu Ala Asp Arg His Leu Gly Val Gly Phe Asp Gln Leu Leu Val Val 
        35                  40                  45              


Glu Pro Pro Tyr Asp Pro Glu Leu Asp Phe His Tyr Arg Ile Phe Asn 
    50                  55                  60                  


Ala Asp Gly Ser Glu Val Ala Gln Cys Gly Asn Gly Ala Arg Cys Phe 
65                  70                  75                  80  


Ala Arg Phe Val Arg Leu Lys Gly Leu Thr Asn Lys Arg Asp Ile Arg 
                85                  90                  95      


Val Ser Thr Ala Asn Gly Arg Met Val Leu Thr Val Thr Asp Asp Asp 
            100                 105                 110         


Leu Val Arg Val Asn Met Gly Glu Pro Asn Phe Glu Pro Ser Ala Val 
        115                 120                 125             


Pro Phe Arg Ala Asn Lys Ala Glu Lys Thr Tyr Ile Met Arg Ala Ala 
    130                 135                 140                 


Glu Gln Thr Ile Leu Cys Gly Val Val Ser Met Gly Asn Pro His Cys 
145                 150                 155                 160 


Val Ile Gln Val Asp Asp Val Asp Thr Ala Ala Val Glu Thr Leu Gly 
                165                 170                 175     


Pro Val Leu Glu Ser His Glu Arg Phe Pro Glu Arg Ala Asn Ile Gly 
            180                 185                 190         


Phe Met Gln Val Val Lys Arg Glu His Ile Arg Leu Arg Val Tyr Glu 
        195                 200                 205             


Arg Gly Ala Gly Glu Thr Gln Ala Cys Gly Ser Gly Ala Cys Ala Ala 
    210                 215                 220                 


Val Ala Val Gly Ile Gln Gln Gly Leu Leu Ala Glu Glu Val Arg Val 
225                 230                 235                 240 


Glu Leu Pro Gly Gly Arg Leu Asp Ile Ala Trp Lys Gly Pro Gly His 
                245                 250                 255     


Pro Leu Tyr Tyr Thr Gly Pro Ala Val His Val Tyr Asp Gly Phe Ile 
            260                 265                 270         


His Leu 
        


<210>  6
<211>  936
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysR_S36R_nt

<400>  6
atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc       60

ctgactgagg cggcacacct gctacacacc tcacagccaa ccgtccgtcg tgagttagcg      120

cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtgggcg attacatcct      180

accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc      240

attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc      300

ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat      360

cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg      420

gcccagcgtc atgatttagg actcactgaa acgctccata cgcctgcggg aacagaacgt      480

accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta      540

aaaaaggtat taacgccgga tgattttcag ggtgagaact acatcagcct ttcccgtact      600

gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg      660

atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtatt      720

tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt ggtgcggcgg      780

ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca      840

gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact ggtcacttct      900

cttgacgcta ttttgtcgtc agctacgaca gcataa                                936


<210>  7
<211>  825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DapF_G210D_nt

<400>  7
atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg       60

cagaatgtct ttttttcacc ggagctgatt cgtcgcctgg ctgatcggca cctgggggta      120

gggtttgacc aactgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat      180

cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt      240

gcccgttttg tgcgtctgaa aggactgacc aataagcgtg atatccgcgt cagcaccgcc      300

aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa      360

cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt      420

atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc      480

gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa      540

agccacgagc gttttccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag      600

catattcgtt tacgcgttta tgaaagagat gcaggagaaa cccaggcctg cggcagcggc      660

gcgtgtgcgg cggttgcagt agggattcag caaggtttgc tggccgaaga agtacgcgtg      720

gaactccccg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg      780

actggcccgg cggtacatgt ctacgacgga tttattcatc tatga                      825


<210>  8
<211>  825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DapF_M260Y_nt

<400>  8
atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg       60

cagaatgtct ttttttcacc ggagctgatt cgtcgcctgg ctgatcggca cctgggggta      120

gggtttgacc aactgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat      180

cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt      240

gcccgttttg tgcgtctgaa aggactgacc aataagcgtg atatccgcgt cagcaccgcc      300

aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa      360

cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt      420

atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc      480

gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa      540

agccacgagc gttttccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag      600

catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc      660

gcgtgtgcgg cggttgcagt agggattcag caaggtttgc tggccgaaga agtacgcgtg      720

gaactccccg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatattat      780

accggtccgg cggtacatgt ctacgacgga tttattcatc tatga                      825


<210>  9
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysP_T33F_nt

<400>  9
atggtttccg aaactaaaac cacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt       60

cacctgacga tgattgccat tggcggttcc atcggctttg gcctttttgt tgcctctggc      120

gcaacgattt ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg      180

atggtttact tcctgatgac cagtctcggt gaactggctg catatatgcc ggtttccggt      240

tcgtttgcca cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg      300

aactactggt acaactgggc ggtgactatc gccgttgacc tggttgcagc tcagctggtc      360

atgagctggt ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt      420

atcttcctgc tgaactacat ctcagttcgt ggctttggtg aagcggaata ctggttctca      480

ctgatcaaag tcacgacagt tattgtcttt atcatcgttg gcgtgctgat gattatcggt      540

atcttcaaag gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt      600

gctggtggtt ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttccaggga      660

accgagctga tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc      720

gcggtacgtc aggtgttctg gcgaatcctg ttgttctatg tgttcgcgat cctgattatc      780

agcctgatta ttccgtacac cgatccgagc ctgctgcgta acgatgttaa agacatcagc      840

gttagtccgt tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg      900

aacgcagtta ttctgacggc ggtgctgtca gcgggtaact ccggtatgta tgcgtctact      960

cgtatgctgt acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaactgtcg     1020

cgtggtggcg tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tctgtgcttc     1080

ctgacctcca tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg     1140

acgggtttta tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac     1200

gtattgcagg gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg     1260

ccgatcttcg cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc     1320

ctgaaagata ctattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc     1380

ctgattattt ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa     1440

atgaagttcc cgcagaacga taagaaataa                                      1470


<210>  10
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysP_Q219I_nt

<400>  10
atggtttccg aaactaaaac cacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt       60

cacctgacga tgattgccat tggcggttcc atcggtacag gtctttttgt tgcctctggc      120

gcaacgattt ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg      180

atggtttact tcctgatgac cagtctcggt gaactggctg catatatgcc ggtttccggt      240

tcgtttgcca cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg      300

aactactggt acaactgggc ggtgactatc gccgttgacc tggttgcagc tcagctggtc      360

atgagctggt ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt      420

atcttcctgc tgaactacat ctcagttcgt ggctttggtg aagcggaata ctggttctca      480

ctgatcaaag tcacgacagt tattgtcttt atcatcgttg gcgtgctgat gattatcggt      540

atcttcaaag gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt      600

gctggtggtt ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttcattggc      660

acggagctga tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc      720

gcggtacgtc aggtgttctg gcgaatcctg ttgttctatg tgttcgcgat cctgattatc      780

agcctgatta ttccgtacac cgatccgagc ctgctgcgta acgatgttaa agacatcagc      840

gttagtccgt tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg      900

aacgcagtta ttctgacggc ggtgctgtca gcgggtaact ccggtatgta tgcgtctact      960

cgtatgctgt acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaactgtcg     1020

cgtggtggcg tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tctgtgcttc     1080

ctgacctcca tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg     1140

acgggtttta tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac     1200

gtattgcagg gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg     1260

ccgatcttcg cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc     1320

ctgaaagata ctattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc     1380

ctgattattt ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa     1440

atgaagttcc cgcagaacga taagaaataa                                      1470


<210>  11
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  11
aggcccgtat att                                                          13


<210>  12
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  12
caggcccgta tattcg                                                       16


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  13
ataatcaatt gttaaatt                                                     18


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  14
gaatcaattg ttaaattatt                                                   20


<210>  15
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  15
ttggtagagg aaag                                                         14


<210>  16
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  16
cggtagagga aagt                                                         14


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  17
cactgccttt ggtagaggaa agtg                                              24


<210>  18
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  18
cacaaaacac tcaattactt actcaattac tta                                    33


<210>  19
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutation sites derived from E. coli

<400>  19
caaacactca attacttaac accaaaacac tcaattactt aaca                        44


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer: 5'

<400>  20
tcgtcggcag cgtcagatgt gtataagaga cag                                    33


<210>  21
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer: 5'

<400>  21
gtctcgtggg ctcggagatg tgtataagag acag                                   34


