                               SEQUENCE LISTING

<110> CHAN ZUCKERBERG BIOHUB, INC.
      THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR 
      UNIVERSITY
 
<120> METHODS AND COMPOSITIONS INVOLVING THERMOSTABLE CAS9 PROTEIN 
      VARIANTS

<130> 103182-1155150-001610WO

<140>
<141>

<150> 62/901,495
<151> 2019-09-17

<150> 62/747,619
<151> 2018-10-18

<160> 20    

<170> PatentIn version 3.5

<210> 1
<211> 1240
<212> PRT
<213> Ignavibacterium sp.

<400> 1
Met Lys Lys Val Leu Gly Leu Asp Leu Gly Val Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Leu Ile Asp Glu Asp Asp Arg Lys Ile Met Gly Met Gly Ser Arg 
            20                  25                  30          


Ile Ile Pro Leu Thr Thr Asp Asp Lys Asp Glu Phe Thr Lys Gly Asn 
        35                  40                  45              


Thr Ile Ser Lys Asn Gln Gln Arg Thr Ile Lys Arg Thr Gln Arg Lys 
    50                  55                  60                  


Gly Tyr Asp Arg Tyr Gln Leu Arg Arg Gln Asn Leu Val Phe Val Leu 
65                  70                  75                  80  


Lys Gln Asn Asn Met Met Pro Asp Ile Glu Leu Val Asn Leu Pro Lys 
                85                  90                  95      


Leu Glu Leu Trp Lys Leu Arg Ser Asp Ala Val Asn Lys Lys Ile Ser 
            100                 105                 110         


Leu Lys Glu Leu Gly Arg Ile Leu Leu His Leu Asn Gln Lys Arg Gly 
        115                 120                 125             


Tyr Lys Ser Ser Arg Ser Glu Ser Asn Leu Asp Lys Lys Asp Thr Glu 
    130                 135                 140                 


Tyr Val Ala Thr Val Lys Asn Arg Tyr Glu Ser Leu Lys Glu Ile Gly 
145                 150                 155                 160 


Leu Thr Ile Gly Gln Lys Phe Phe Glu Glu Leu Ser Lys Asn Asn Phe 
                165                 170                 175     


Tyr Arg Ile Lys Glu Gln Val Tyr Pro Arg Glu Ala Tyr Val Glu Glu 
            180                 185                 190         


Tyr Asn Lys Ile Met Lys His Gln Gln Lys His Tyr Pro Glu Asn Ile 
        195                 200                 205             


Ser Glu Glu Leu Ile Asn Lys Ile Arg Asp Glu Ile Ile Tyr Tyr Gln 
    210                 215                 220                 


Arg Lys Leu Lys Ser Gln Lys Gly Leu Val Ser Val Cys Glu Phe Glu 
225                 230                 235                 240 


Gly Phe Trp Ile Lys Leu Asn Ser Asn Gly Lys Glu Lys Asp Leu Phe 
                245                 250                 255     


Val Gly Pro Lys Val Thr Pro Lys Ser Ser Pro Leu Phe Gln Val Ser 
            260                 265                 270         


Arg Ile Trp Glu Thr Ile Asn Asn Ile Ser Ile Lys Arg Lys Thr Gly 
        275                 280                 285             


Glu Ser Ile Glu Ile Thr Leu Asp Lys Lys Lys Glu Ile Phe Ala Tyr 
    290                 295                 300                 


Met Asp Lys Asn Glu Lys Leu Ser Tyr Pro Glu Leu Leu Lys Ile Leu 
305                 310                 315                 320 


Gly Leu Lys Lys Asp Asp Val Tyr Gly Asn Lys Asn Leu Thr Asn Gly 
                325                 330                 335     


Leu Leu Gly Asn Lys Ile Lys Thr Glu Met Met Lys Cys Ile Ser Asp 
            340                 345                 350         


Ile Asp Lys Tyr Ser Asp Leu Phe Arg Leu Glu Leu Glu Ile Lys Glu 
        355                 360                 365             


Phe Asp Glu Glu Val Tyr Leu Tyr Asp Arg Thr Thr Gly Glu Ile Ile 
    370                 375                 380                 


Asn Ser Lys Lys Lys Lys Asn Ile Ile Ala Ala Ile Glu Asp Gln Pro 
385                 390                 395                 400 


Phe Tyr Lys Leu Trp His Val Val Tyr Ser Ile Pro Asp Lys Glu Thr 
                405                 410                 415     


Cys Gln Lys Ile Leu Met Ser Lys Phe Gly Ile Gln Glu Glu Asp Ala 
            420                 425                 430         


Ala Lys Leu Ala Thr Leu Asp Phe Thr Lys Leu Gly Phe Ser Asn Lys 
        435                 440                 445             


Ser His Arg Ala Ile Arg Lys Met Leu Pro Tyr Leu Met Glu Gly Asp 
    450                 455                 460                 


Asn Asp Tyr Met Ala Arg Cys Tyr Ala Gly Tyr His His Thr Thr Thr 
465                 470                 475                 480 


Ile Thr Lys Gln Glu Asn Phe Gln Arg Lys Leu Leu Asp Lys Leu Lys 
                485                 490                 495     


Asn Leu Glu Lys Asn Ser Leu Arg Gln Pro Ile Val Glu Lys Ile Leu 
            500                 505                 510         


Asn Gln Met Ile Asn Val Val Asn Ala Ile Ile Asp Lys Tyr Gly Lys 
        515                 520                 525             


Pro Asp Glu Ile Arg Ile Glu Leu Ala Arg Glu Leu Lys Gln Ser Arg 
    530                 535                 540                 


Glu Glu Arg Asn Glu Ala Tyr Arg Asn Met Asn Glu Arg Glu Arg Glu 
545                 550                 555                 560 


Asn Lys Ile Ile Glu Lys Glu Leu Ser Glu Phe Gly Leu Arg Ala Thr 
                565                 570                 575     


Arg Asn Asn Ile Ile Lys Trp Arg Leu Tyr His Glu Ile Ser Asn Glu 
            580                 585                 590         


Glu Lys Lys Gln Asn Ala Ile Cys Ile Tyr Cys Gly Lys Pro Ile Ser 
        595                 600                 605             


Phe Thr Ala Ala Ile Leu Gly Glu Glu Val Glu Val Glu His Ile Ile 
    610                 615                 620                 


Pro Arg Ser Arg Leu Phe Asp Asp Ser Gln Ser Asn Lys Thr Leu Ala 
625                 630                 635                 640 


His Arg Lys Cys Asn Ala Asp Lys Lys Asp Gln Thr Ala Tyr Asp Phe 
                645                 650                 655     


Met Arg Ser Lys Ser Asp Thr Glu Phe Asn Asp Tyr Val Glu Arg Ile 
            660                 665                 670         


Asn Thr Leu Tyr Lys Asn His Val Ile Gly Lys Thr Lys Arg Asp Lys 
        675                 680                 685             


Leu Leu Met Ser Glu Glu Lys Ile Pro Met Asp Phe Ile Asp Arg Gln 
    690                 695                 700                 


Leu Arg Gln Thr Gln Tyr Ile Ser Lys Lys Ala Leu Glu Leu Leu Gln 
705                 710                 715                 720 


Asn Ile Cys Tyr Asn Val Trp Ala Thr Ser Gly Asn Val Thr Ala Glu 
                725                 730                 735     


Leu Arg His Ile Trp Gly Trp Asp Glu Val Leu Glu Asn Leu Gln Leu 
            740                 745                 750         


Pro Lys Tyr Arg Glu Ser Gly Leu Ile Glu Ile Ile Glu Val Gly Asp 
        755                 760                 765             


Lys Asp Asn Lys Gln Lys Lys Glu Lys Ile Ile Gly Trp Thr Lys Arg 
    770                 775                 780                 


Asp Asp His Arg His His Ala Ile Asp Ala Leu Thr Ile Ala Cys Thr 
785                 790                 795                 800 


Lys Gln Gly Phe Ile Gln Arg Phe Asn Arg Leu Asn Ser Gly Lys Val 
                805                 810                 815     


Arg Asn Asp Met Leu Gln Glu Ile Glu Asn Ala Lys Gln Asn Tyr Asp 
            820                 825                 830         


Lys Arg Lys Asn Leu Leu Glu Asn Tyr Ile Leu Ser Tyr Arg Pro Phe 
        835                 840                 845             


Thr Thr Lys Glu Val Glu Arg Glu Ala Glu Lys Ile Leu Val Ser Phe 
    850                 855                 860                 


Lys Ala Gly Lys Lys Val Ala Ser Thr Gly Lys Arg Lys Ile Lys Lys 
865                 870                 875                 880 


Asp Gly Lys Lys Ile Ile Ala Gln Thr Gly Ile Ile Ile Pro Arg Gly 
                885                 890                 895     


Pro Leu Ser Glu Glu Ser Val Tyr Gly Lys Ile Lys Val Ile Glu Lys 
            900                 905                 910         


Glu Lys Pro Leu Lys Tyr Leu Phe Glu Asn Pro His Leu Ile Phe Lys 
        915                 920                 925             


Pro Asn Ile Lys Ala Leu Val Glu Glu Arg Leu Tyr Lys Asn Asn Asn 
    930                 935                 940                 


Asp Pro Lys Ser Ala Ile Ala Ser Leu Lys Lys Glu Pro Ile Tyr Leu 
945                 950                 955                 960 


Asp Lys Glu Lys Thr Ile Lys Leu Glu Tyr Gly Thr Cys Tyr Lys Glu 
                965                 970                 975     


Glu Val Val Ile Lys Lys Pro Leu Gln Ala Leu Asn Glu Lys Gln Val 
            980                 985                 990         


Glu Asp Ile Val Asp Pro Ile Ile  Lys Gln Lys Ile Lys  Asp Arg Leu 
        995                 1000                 1005             


Val Lys  Phe Gly Gly Lys Ala  Lys Glu Ala Phe Lys  Asp Leu Glu 
    1010                 1015                 1020             


Asn Glu  Pro Ile Trp Tyr Asp  Glu Glu Lys Arg Ile  Pro Ile Lys 
    1025                 1030                 1035             


Asn Val  Arg Trp Phe Thr Gly  Leu Ser Ala Ile Glu  Pro Ile Ser 
    1040                 1045                 1050             


Lys Asp  Glu Thr Gly Lys Glu  Ile Gly Phe Val Lys  Pro Gly Asn 
    1055                 1060                 1065             


Asn His  His Leu Ala Ile Tyr  Ile Asp Glu Glu Gly  Lys Lys Gln 
    1070                 1075                 1080             


Leu Ser  Ile Cys Ser Phe Trp  His Ala Val Glu Arg  Lys Lys Tyr 
    1085                 1090                 1095             


Gly Leu  Pro Val Ile Ile Lys  Asn Pro Ser Glu Val  Val Asp Phe 
    1100                 1105                 1110             


Ile Leu  Ala Glu Glu Asn Glu  Asp Lys Tyr Pro Glu  Ser Phe Leu 
    1115                 1120                 1125             


Glu Lys  Leu Pro Ala Gly Lys  Trp Thr Phe Lys Glu  Ser Phe Gln 
    1130                 1135                 1140             


Gln Asn  Glu Met Phe Val Leu  Gly Ile Ser Lys Glu  Ala Phe Glu 
    1145                 1150                 1155             


Glu Ala  Ile Ser Arg Asn Asp  Tyr Ser Phe Leu Ser  Asn Tyr Leu 
    1160                 1165                 1170             


Tyr Arg  Val Gln Lys Ile Ala  Met Ile Gly Lys Gln  Pro Asn Ile 
    1175                 1180                 1185             


Val Phe  Arg His His Leu Glu  Thr Gln Leu Lys Asp  Asp Ala Tyr 
    1190                 1195                 1200             


Ala Lys  Lys Ser Asn Arg Phe  Tyr Leu Ile Gln Ser  Ile Gly Ala 
    1205                 1210                 1215             


Leu Glu  Ser Leu Tyr Pro Ile  Lys Ile Leu Ile Asn  Cys Leu Gly 
    1220                 1225                 1230             


Glu Ile  Ile Thr Asn Asn Lys  
    1235                 1240 


<210> 2
<211> 3723
<212> DNA
<213> Ignavibacterium sp.

<400> 2
atgaaaaaag tattaggatt agatcttgga gtatcttcaa taggctgggc tttaattgac       60

gaagatgata gaaaaataat gggcatgggt agtagaataa taccattaac aactgatgat      120

aaagacgagt ttacaaaagg caatacgatt tctaagaatc agcaacgaac aattaaaaga      180

actcaaagaa aaggatacga tcgttatcaa ttaagaaggc agaatttagt tttcgtgttg      240

aaacaaaata atatgatgcc tgatattgaa ttagtaaatc ttccaaaact tgaattatgg      300

aaactaagaa gtgatgcggt taataaaaaa atatctttga aagaattagg cagaatccta      360

cttcacttaa atcaaaaaag aggttataaa agtagcagaa gtgaatcaaa tttggataag      420

aaagataccg aatatgtagc aacagtaaaa aacagatatg aaagcctaaa agaaattggt      480

ttaacaatag gacagaaatt ttttgaggaa ttatccaaaa acaattttta cagaataaaa      540

gaacaggttt acccaagaga agcatatgtt gaagagtata ataaaataat gaagcatcaa      600

caaaaacatt atccagaaaa tatttcggaa gaattaatta ataaaataag agacgaaata      660

atttactatc aacgaaaact aaaatcgcaa aagggattgg tgtctgtttg cgagtttgaa      720

ggattttgga taaagctaaa ttcaaatgga aaagaaaaag atttatttgt tggtccaaaa      780

gtaactccta aaagttcacc attattccag gtaagtagaa tttgggaaac tatcaataac      840

atatcaatta aaagaaagac tggtgaatcc attgaaatta cactggataa aaagaaagaa      900

atttttgctt atatggataa aaatgaaaaa ttaagctatc cagaattatt aaaaatttta      960

gggcttaaaa aagatgacgt atatggaaac aagaatttaa caaatgggtt gctgggcaac     1020

aaaataaaaa cagaaatgat gaagtgtatt tcagatattg ataagtattc tgatttattc     1080

cgattagaac ttgaaataaa agaattcgat gaagaggttt atttatatga tagaacaacc     1140

ggagaaataa taaattcaaa gaaaaaaaag aatataatag cagcaataga agaccaacca     1200

ttttacaagc tttggcatgt tgtttattca atacccgata aagaaacttg tcaaaaaata     1260

cttatgtcaa aatttggcat acaggaagaa gacgctgcta aattagcaac acttgatttt     1320

actaaacttg gtttttcgaa caaatcccac cgtgcaatta ggaaaatgct tccttatcta     1380

atggaagggg ataacgatta tatggcccgt tgttatgcgg gttatcatca cacaacaaca     1440

attacaaaac aagaaaactt ccaaagaaaa ctgttagata aattaaaaaa cttagaaaaa     1500

aatagcctgc gccagccgat agttgaaaaa attctaaatc agatgataaa tgttgtaaat     1560

gcaattatag acaaatatgg gaaaccggat gaaattagaa ttgaactagc cagagaatta     1620

aaacagagta gagaagaaag aaatgaagca tatagaaaca tgaatgaacg agaacgtgaa     1680

aataaaataa ttgaaaaaga gctttctgaa tttggacttc gtgcaacacg aaacaatatt     1740

atcaaatgga gattatatca cgaaattagc aacgaagaaa agaaacaaaa tgcaatttgc     1800

atttattgtg gcaaaccaat ttcctttact gctgcaatat taggtgaaga agttgaagtt     1860

gaacacataa taccaaggtc aaggttattt gacgattctc aaagcaataa aacactggca     1920

catagaaaat gcaatgcaga taagaaagac caaacagctt atgactttat gcgttcaaaa     1980

tctgatactg aatttaatga ttacgttgag cgaattaata ccctttataa aaatcatgta     2040

attggaaaaa cgaaaagaga taaactttta atgtctgaag aaaaaattcc tatggatttt     2100

attgacagac aattaagaca aacacaatac atctctaaaa aagcattaga gcttcttcag     2160

aatatctgtt ataatgtgtg ggcaacaagc ggaaatgtga ccgccgagtt gcgccatata     2220

tggggatggg atgaagtgct tgaaaatctt caattaccta agtatagaga aagtggatta     2280

atagaaatta ttgaagttgg agataaagat aataaacaaa aaaaggaaaa gataattgga     2340

tggaccaaaa gagacgatca tagacatcat gcaattgatg ctcttaccat cgcatgtacc     2400

aaacaaggat ttatccaacg ctttaataga ttaaatagtg ggaaagtacg aaacgatatg     2460

cttcaggaaa ttgaaaacgc caaacagaat tacgataaaa gaaaaaatct tttggagaac     2520

tatattcttt cttacagacc atttacaaca aaggaagttg aaagagaggc tgagaaaata     2580

cttgtatcat tcaaagccgg caaaaaggtt gcatctacag gcaaaagaaa aattaaaaaa     2640

gatggcaaaa aaataatcgc tcaaactggt attattattc caagaggacc attaagtgaa     2700

gaaagtgtct atggaaaaat aaaagtaatt gagaaggaaa aaccgttaaa atatttattt     2760

gaaaatccac acctcatatt taaaccaaat ataaaagcac ttgtagaaga aagactttac     2820

aaaaacaata acgaccctaa aagtgctata gcttcattaa aaaaagaacc tatttatctt     2880

gacaaagaga aaacaataaa attggaatac ggaacatgtt ataaagaaga agttgttata     2940

aaaaaaccac tacaagcttt gaacgagaag caagtagagg atattgttga ccctataata     3000

aaacaaaaga ttaaggatcg actggttaaa tttggtggca aagccaaaga agcatttaag     3060

gatttagaaa acgaacctat ttggtatgat gaggaaaaaa gaattccaat aaagaatgtt     3120

cgatggttta caggactttc agcaattgaa cctataagca aggatgagac cggaaaagaa     3180

attggatttg tcaaacctgg caataatcat catcttgcaa tatacattga tgaagaaggg     3240

aaaaaacaac ttagtatatg ttcattttgg catgctgtag aaagaaagaa atatgggttg     3300

cctgttataa taaaaaatcc gtcagaggtt gttgatttta tacttgcgga ggaaaatgaa     3360

gataaatatc cagaaagttt tctagaaaaa ttacccgctg ggaaatggac atttaaagaa     3420

agctttcaac aaaacgagat gtttgtactt ggaataagca aagaagcatt tgaagaagcc     3480

atttcgagaa atgattatag cttcttaagt aattacttat atcgtgttca aaagattgca     3540

atgataggca aacaaccaaa tattgttttt agacatcatc tcgaaactca gcttaaggat     3600

gacgcatacg ctaaaaaaag taatcgcttt tatttaatac aaagtatcgg ggcattagaa     3660

tcattatatc caataaaaat tttaattaat tgtttgggag aaattattac taataataaa     3720

taa                                                                   3723


<210> 3
<211> 3723
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
atgaagaagg tcctgggctt agacctgggt gtgagctcga ttggttgggc gctgattgac       60

gaagacgacc gcaagattat gggaatggga tcccgtatca ttccgctgac caccgatgat      120

aaggatgagt ttacaaaggg taacacaatc agcaaaaatc agcagcgcac catcaagcgc      180

acgcaacgta agggatatga tcgttatcag ctgcgccgcc agaatctggt gtttgtttta      240

aaacaaaata acatgatgcc cgatattgag ctggttaacc tgcccaagct ggaactgtgg      300

aaactgcgtt ctgatgctgt aaataagaaa atctctttaa aagaactggg ccgtatcctg      360

ttacacctga atcagaaacg tggttataaa tcatctcgct ctgagtcaaa cctggacaag      420

aaggatacag agtatgttgc tacggtcaaa aatcgttatg aaagcttaaa ggagatcggc      480

ttaacgattg gccagaagtt cttcgaagag ttatcgaaga acaattttta tcgcatcaag      540

gaacaggtct atccgcgtga agcctacgtc gaggaatata ataaaatcat gaaacaccaa      600

cagaaacatt accccgagaa tatttcggag gaactgatta acaagatccg tgacgaaatc      660

atttactacc aacgcaaact gaaatctcag aaaggactgg tgtcggtatg cgagtttgag      720

ggattttgga tcaaactgaa ctcgaatggt aaggaaaaag atttatttgt cggtccaaag      780

gtaacaccta agtcttctcc gctgttccag gtctctcgta tctgggagac tatcaacaac      840

atcagtatta aacgtaagac gggtgagtcc attgaaatta cgctggacaa gaaaaaagaa      900

atcttcgcct acatggacaa aaatgaaaag ctgagttacc ctgagctgct gaaaattctg      960

ggtctgaaga aggacgacgt ttatggcaac aaaaatctga ccaacggctt attaggtaat     1020

aagatcaaaa ccgaaatgat gaaatgtatt tccgacatcg ataagtattc agacctgttt     1080

cgcctggagc tggagattaa ggagttcgac gaggaagtct acttatacga tcgcactacc     1140

ggtgaaatca tcaactcgaa gaagaaaaaa aatatcattg cggcgattga agaccaacct     1200

ttctataaac tgtggcatgt ggtatactcg attcccgaca aggagacctg ccagaaaatt     1260

ctgatgtcta agttcggcat tcaggaggag gacgcagcta aactggcgac gctggatttc     1320

accaaactgg ggttttccaa taagtcacat cgcgcgattc gcaaaatgct gccgtactta     1380

atggagggcg ataacgacta tatggcacgt tgttatgctg gttatcatca tacaacaacc     1440

attacgaaac aagagaattt tcaacgcaaa ttactggata agttaaaaaa tctggaaaaa     1500

aatagcctgc gtcagccaat tgtggagaaa atcctgaacc aaatgattaa tgttgtcaat     1560

gccattatcg ataagtatgg taaacccgat gaaatccgca ttgaattagc gcgtgaactg     1620

aagcagtctc gcgaggaacg taacgaagcc taccgtaata tgaacgaacg tgagcgtgaa     1680

aacaaaatta tcgagaagga actgagtgaa ttcggcctgc gtgccacgcg taacaatatt     1740

atcaaatggc gcctgtacca cgagatttct aatgaagaga aaaagcagaa tgctatttgt     1800

atctactgtg gaaagcctat ttcatttaca gctgcgattc tgggagagga agtagaagtt     1860

gaacacatca tccctcgtag tcgcctgttc gatgactcgc agagcaataa gaccctggcg     1920

catcgcaagt gcaatgctga taagaaggac cagaccgcat acgattttat gcgttcgaag     1980

tctgatactg aatttaacga ctacgtagag cgcatcaata ccctgtacaa aaaccacgtc     2040

attgggaaaa ctaagcgcga caaactgctg atgtccgagg agaaaattcc aatggacttc     2100

atcgatcgtc aactgcgcca gactcaatac atttccaaga aggcactgga gctgctgcag     2160

aacatttgct acaatgtttg ggctactagc ggcaatgtta ccgcagaact gcgtcacatt     2220

tggggctggg atgaggttct ggaaaacctg cagctgccta agtaccgtga atccggctta     2280

attgaaatta tcgaagttgg agacaaggac aataagcaga aaaaagagaa gatcattggc     2340

tggactaagc gcgacgatca tcgccatcat gctattgacg cactgacaat tgcgtgtacc     2400

aagcagggtt tcatccagcg ttttaatcgt ctgaacagtg ggaaggtccg taatgacatg     2460

ctgcaggaaa tcgagaatgc gaaacagaac tacgataagc gcaaaaactt actggaaaac     2520

tacattctgt cttatcgtcc tttcactact aaagaagttg agcgcgaggc agaaaaaatc     2580

ttggtctctt tcaaggcggg aaaaaaagtc gcgtcgactg gtaaacgcaa gatcaagaaa     2640

gatggtaaga agattatcgc gcaaacaggg atcatcatcc cacgcggtcc actgagcgaa     2700

gagagcgtct acggaaaaat caaggtcatc gaaaaggaaa aaccactgaa atatctgttt     2760

gaaaatccac atctgatttt taaacccaat atcaaggcac tggttgaaga gcgtctgtac     2820

aaaaacaaca atgacccgaa aagtgctatc gcgtcattaa agaaggagcc aatttattta     2880

gacaaggaga agaccattaa actggagtat gggacgtgct acaaggaaga ggtcgtcatc     2940

aagaagccgt tacaagccct gaatgagaaa caagtagagg acatcgtcga tccgatcatt     3000

aagcaaaaga tcaaggaccg cctggtgaag ttcggcggta aggcaaaaga agcatttaag     3060

gatctggaaa acgagccgat ctggtacgat gaggagaagc gcatcccgat caagaacgta     3120

cgctggttca ctggtctgtc ggctatcgag ccgatcagca aagatgaaac cggtaaggag     3180

attgggtttg tcaaacctgg taacaatcac catctggcga tttacattga cgaggagggg     3240

aagaagcagc tgagcatctg tagtttttgg catgccgtcg agcgtaaaaa atacggactg     3300

cctgtaatca ttaaaaaccc atctgaagtg gttgatttca ttctggccga ggaaaatgaa     3360

gacaagtatc cagagtcctt tttagagaag ctgcccgcgg ggaagtggac attcaaagag     3420

tcgttccagc aaaacgagat gttcgtcctg ggtatctcaa aagaagcatt cgaagaggca     3480

atttcgcgca atgattatag cttcttatcg aattacctgt accgtgtgca aaaaattgct     3540

atgatcggga agcagcccaa tatcgttttt cgccatcatc tggagaccca actgaaggac     3600

gacgcgtatg ccaaaaagtc gaatcgtttt tacctgatcc agagtattgg tgccttagaa     3660

tctttatatc ctattaaaat tctgattaat tgcctgggag agattatcac taataacaag     3720

taa                                                                   3723


<210> 4
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
ccacatcgaa                                                              10


<210> 5
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
agacatgaaa                                                              10


<210> 6
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (4)..(4)
<223> a, c, t, g, unknown or other

<400> 6
nvrnat                                                                   6


<210> 7
<211> 106
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 7
guugugauuu gcuuucaaag aaauuugaag caaaucacaa uaaggauuuu uccguuguga       60

aaacauuuac aguagucccg augcaaacca ucgggauugu uguuuu                     106


<210> 8
<211> 99
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
catggtcaga caagcttact agtaaaggat ccacgggtac cgagcttcca tccgggaata       60

gttacattac tatctgtagg acatgaaaga attcgtaat                              99


<210> 9
<211> 131
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 9
gggaauaguu acauuacuau cuguaguugu gauuugcuuu caaagaaauu ugaagcaaau       60

cacaauaagg auuuuuccgu ugugaaaaca uuuacaguag ucccgaugca aaccaucggg      120

auuguuguuu u                                                           131


<210> 10
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
ggacat                                                                   6


<210> 11
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
gggaatagtt acattactat ctgta                                             25


<210> 12
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
agacat                                                                   6


<210> 13
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (4)..(4)
<223> a, c, t, g, unknown or other

<400> 13
nrrnat                                                                   6


<210> 14
<211> 1368
<212> PRT
<213> Streptococcus pyogenes

<400> 14
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210> 15
<211> 1409
<212> PRT
<213> Streptococcus thermophilus

<400> 15
Met Leu Phe Asn Lys Cys Ile Ile Ile Ser Ile Asn Leu Asp Phe Ser 
1               5                   10                  15      


Asn Lys Glu Lys Cys Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile 
            20                  25                  30          


Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Asn Tyr Lys Val 
        35                  40                  45              


Pro Ser Lys Lys Met Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile 
    50                  55                  60                  


Lys Lys Asn Leu Leu Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala 
65                  70                  75                  80  


Glu Gly Arg Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg 
                85                  90                  95      


Arg Asn Arg Ile Leu Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala 
            100                 105                 110         


Thr Leu Asp Asp Ala Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val 
        115                 120                 125             


Pro Asp Asp Lys Arg Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val 
    130                 135                 140                 


Glu Glu Lys Val Tyr His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg 
145                 150                 155                 160 


Lys Tyr Leu Ala Asp Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr 
                165                 170                 175     


Leu Ala Leu Ala His Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu 
            180                 185                 190         


Gly Glu Phe Asn Ser Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp 
        195                 200                 205             


Phe Leu Asp Thr Tyr Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu 
    210                 215                 220                 


Asn Ser Lys Gln Leu Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu 
225                 230                 235                 240 


Glu Lys Lys Asp Arg Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser 
                245                 250                 255     


Gly Ile Phe Ser Glu Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp 
            260                 265                 270         


Phe Arg Lys Cys Phe Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser 
        275                 280                 285             


Lys Glu Ser Tyr Asp Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly 
    290                 295                 300                 


Asp Asp Tyr Ser Asp Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala 
305                 310                 315                 320 


Ile Leu Leu Ser Gly Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala 
                325                 330                 335     


Pro Leu Ser Ser Ala Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp 
            340                 345                 350         


Leu Ala Leu Leu Lys Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr 
        355                 360                 365             


Asn Glu Val Phe Lys Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile 
    370                 375                 380                 


Asp Gly Lys Thr Asn Gln Glu Asp Phe Tyr Val Tyr Leu Lys Asn Leu 
385                 390                 395                 400 


Leu Ala Glu Phe Glu Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg 
                405                 410                 415     


Glu Asp Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro 
            420                 425                 430         


Tyr Gln Ile His Leu Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala 
        435                 440                 445             


Lys Phe Tyr Pro Phe Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile 
    450                 455                 460                 


Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn 
465                 470                 475                 480 


Ser Asp Phe Ala Trp Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro 
                485                 490                 495     


Trp Asn Phe Glu Asp Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe 
            500                 505                 510         


Ile Asn Arg Met Thr Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val 
        515                 520                 525             


Leu Pro Lys His Ser Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu 
    530                 535                 540                 


Leu Thr Lys Val Arg Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe 
545                 550                 555                 560 


Leu Asp Ser Lys Gln Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp 
                565                 570                 575     


Lys Arg Lys Val Thr Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile 
            580                 585                 590         


Tyr Gly Tyr Asp Gly Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn 
        595                 600                 605             


Ser Ser Leu Ser Thr Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys 
    610                 615                 620                 


Glu Phe Leu Asp Asp Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile 
625                 630                 635                 640 


His Thr Leu Thr Ile Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu 
                645                 650                 655     


Ser Lys Phe Glu Asn Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser 
            660                 665                 670         


Arg Arg His Tyr Thr Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn 
        675                 680                 685             


Gly Ile Arg Asp Glu Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile 
    690                 695                 700                 


Asp Asp Gly Ile Ser Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp 
705                 710                 715                 720 


Ala Leu Ser Phe Lys Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp 
                725                 730                 735     


Glu Asp Lys Gly Asn Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser 
            740                 745                 750         


Pro Ala Ile Lys Lys Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu 
        755                 760                 765             


Leu Val Lys Val Met Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu 
    770                 775                 780                 


Met Ala Arg Glu Asn Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln 
785                 790                 795                 800 


Gln Arg Leu Lys Arg Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys 
                805                 810                 815     


Ile Leu Lys Glu Asn Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn 
            820                 825                 830         


Ala Leu Gln Asn Asp Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys 
        835                 840                 845             


Asp Met Tyr Thr Gly Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr 
    850                 855                 860                 


Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile 
865                 870                 875                 880 


Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp 
                885                 890                 895     


Asp Phe Pro Ser Leu Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr 
            900                 905                 910         


Gln Leu Leu Lys Ser Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu 
        915                 920                 925             


Thr Lys Ala Glu Arg Gly Gly Leu Leu Pro Glu Asp Lys Ala Gly Phe 
    930                 935                 940                 


Ile Gln Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys His Val Ala 
945                 950                 955                 960 


Arg Leu Leu Asp Glu Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg 
                965                 970                 975     


Ala Val Arg Thr Val Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser 
            980                 985                 990         


Gln Phe Arg Lys Asp Phe Glu Leu  Tyr Lys Val Arg Glu  Ile Asn Asp 
        995                 1000                 1005             


Phe His  His Ala His Asp Ala  Tyr Leu Asn Ala Val  Ile Ala Ser 
    1010                 1015                 1020             


Ala Leu  Leu Lys Lys Tyr Pro  Lys Leu Glu Pro Glu  Phe Val Tyr 
    1025                 1030                 1035             


Gly Asp  Tyr Pro Lys Tyr Asn  Ser Phe Arg Glu Arg  Lys Ser Ala 
    1040                 1045                 1050             


Thr Glu  Lys Val Tyr Phe Tyr  Ser Asn Ile Met Asn  Ile Phe Lys 
    1055                 1060                 1065             


Lys Ser  Ile Ser Leu Ala Asp  Gly Arg Val Ile Glu  Arg Pro Leu 
    1070                 1075                 1080             


Ile Glu  Val Asn Glu Glu Thr  Gly Glu Ser Val Trp  Asn Lys Glu 
    1085                 1090                 1095             


Ser Asp  Leu Ala Thr Val Arg  Arg Val Leu Ser Tyr  Pro Gln Val 
    1100                 1105                 1110             


Asn Val  Val Lys Lys Val Glu  Glu Gln Asn His Gly  Leu Asp Arg 
    1115                 1120                 1125             


Gly Lys  Pro Lys Gly Leu Phe  Asn Ala Asn Leu Ser  Ser Lys Pro 
    1130                 1135                 1140             


Lys Pro  Asn Ser Asn Glu Asn  Leu Val Gly Ala Lys  Glu Tyr Leu 
    1145                 1150                 1155             


Asp Pro  Lys Lys Tyr Gly Gly  Tyr Ala Gly Ile Ser  Asn Ser Phe 
    1160                 1165                 1170             


Ala Val  Leu Val Lys Gly Thr  Ile Glu Lys Gly Ala  Lys Lys Lys 
    1175                 1180                 1185             


Ile Thr  Asn Val Leu Glu Phe  Gln Gly Ile Ser Ile  Leu Asp Arg 
    1190                 1195                 1200             


Ile Asn  Tyr Arg Lys Asp Lys  Leu Asn Phe Leu Leu  Glu Lys Gly 
    1205                 1210                 1215             


Tyr Lys  Asp Ile Glu Leu Ile  Ile Glu Leu Pro Lys  Tyr Ser Leu 
    1220                 1225                 1230             


Phe Glu  Leu Ser Asp Gly Ser  Arg Arg Met Leu Ala  Ser Ile Leu 
    1235                 1240                 1245             


Ser Thr  Asn Asn Lys Arg Gly  Glu Ile His Lys Gly  Asn Gln Ile 
    1250                 1255                 1260             


Phe Leu  Ser Gln Lys Phe Val  Lys Leu Leu Tyr His  Ala Lys Arg 
    1265                 1270                 1275             


Ile Ser  Asn Thr Ile Asn Glu  Asn His Arg Lys Tyr  Val Glu Asn 
    1280                 1285                 1290             


His Lys  Lys Glu Phe Glu Glu  Leu Phe Tyr Tyr Ile  Leu Glu Phe 
    1295                 1300                 1305             


Asn Glu  Asn Tyr Val Gly Ala  Lys Lys Asn Gly Lys  Leu Leu Asn 
    1310                 1315                 1320             


Ser Ala  Phe Gln Ser Trp Gln  Asn His Ser Ile Asp  Glu Leu Cys 
    1325                 1330                 1335             


Ser Ser  Phe Ile Gly Pro Thr  Gly Ser Glu Arg Lys  Gly Leu Phe 
    1340                 1345                 1350             


Glu Leu  Thr Ser Arg Gly Ser  Ala Ala Asp Phe Glu  Phe Leu Gly 
    1355                 1360                 1365             


Val Lys  Ile Pro Arg Tyr Arg  Asp Tyr Thr Pro Ser  Ser Leu Leu 
    1370                 1375                 1380             


Lys Asp  Ala Thr Leu Ile His  Gln Ser Val Thr Gly  Leu Tyr Glu 
    1385                 1390                 1395             


Thr Arg  Ile Asp Leu Ala Lys  Leu Gly Glu Gly 
    1400                 1405                 


<210> 16
<211> 1059
<212> PRT
<213> Wolinella succinogenes

<400> 16
Met Ile Glu Arg Ile Leu Gly Val Asp Leu Gly Ile Ser Ser Leu Gly 
1               5                   10                  15      


Trp Ala Ile Val Glu Tyr Asp Lys Asp Asp Glu Ala Ala Asn Arg Ile 
            20                  25                  30          


Ile Asp Cys Gly Val Arg Leu Phe Thr Ala Ala Glu Thr Pro Lys Lys 
        35                  40                  45              


Lys Glu Ser Pro Asn Lys Ala Arg Arg Glu Ala Arg Gly Ile Arg Arg 
    50                  55                  60                  


Val Leu Asn Arg Arg Arg Val Arg Met Asn Met Ile Lys Lys Leu Phe 
65                  70                  75                  80  


Leu Arg Ala Gly Leu Ile Gln Asp Val Asp Leu Asp Gly Glu Gly Gly 
                85                  90                  95      


Met Phe Tyr Ser Lys Ala Asn Arg Ala Asp Val Trp Glu Leu Arg His 
            100                 105                 110         


Asp Gly Leu Tyr Arg Leu Leu Lys Gly Asp Glu Leu Ala Arg Val Leu 
        115                 120                 125             


Ile His Ile Ala Lys His Arg Gly Tyr Lys Phe Ile Gly Asp Asp Glu 
    130                 135                 140                 


Ala Asp Glu Glu Ser Gly Lys Val Lys Lys Ala Gly Val Val Leu Arg 
145                 150                 155                 160 


Gln Asn Phe Glu Ala Ala Gly Cys Arg Thr Val Gly Glu Trp Leu Trp 
                165                 170                 175     


Arg Glu Arg Gly Ala Asn Gly Lys Lys Arg Asn Lys His Gly Asp Tyr 
            180                 185                 190         


Glu Ile Ser Ile His Arg Asp Leu Leu Val Glu Glu Val Glu Ala Ile 
        195                 200                 205             


Phe Val Ala Gln Gln Glu Met Arg Ser Thr Ile Ala Thr Asp Ala Leu 
    210                 215                 220                 


Lys Ala Ala Tyr Arg Glu Ile Ala Phe Phe Val Arg Pro Met Gln Arg 
225                 230                 235                 240 


Ile Glu Lys Met Val Gly His Cys Thr Tyr Phe Pro Glu Glu Arg Arg 
                245                 250                 255     


Ala Pro Lys Ser Ala Pro Thr Ala Glu Lys Phe Ile Ala Ile Ser Lys 
            260                 265                 270         


Phe Phe Ser Thr Val Ile Ile Asp Asn Glu Gly Trp Glu Gln Lys Ile 
        275                 280                 285             


Ile Glu Arg Lys Thr Leu Glu Glu Leu Leu Asp Phe Ala Val Ser Arg 
    290                 295                 300                 


Glu Lys Val Glu Phe Arg His Leu Arg Lys Phe Leu Asp Leu Ser Asp 
305                 310                 315                 320 


Asn Glu Ile Phe Lys Gly Leu His Tyr Lys Gly Lys Pro Lys Thr Ala 
                325                 330                 335     


Lys Lys Arg Glu Ala Thr Leu Phe Asp Pro Asn Glu Pro Thr Glu Leu 
            340                 345                 350         


Glu Phe Asp Lys Val Glu Ala Glu Lys Lys Ala Trp Ile Ser Leu Arg 
        355                 360                 365             


Gly Ala Ala Lys Leu Arg Glu Ala Leu Gly Asn Glu Phe Tyr Gly Arg 
    370                 375                 380                 


Phe Val Ala Leu Gly Lys His Ala Asp Glu Ala Thr Lys Ile Leu Thr 
385                 390                 395                 400 


Tyr Tyr Lys Asp Glu Gly Gln Lys Arg Arg Glu Leu Thr Lys Leu Pro 
                405                 410                 415     


Leu Glu Ala Glu Met Val Glu Arg Leu Val Lys Ile Gly Phe Ser Asp 
            420                 425                 430         


Phe Leu Lys Leu Ser Leu Lys Ala Ile Arg Asp Ile Leu Pro Ala Met 
        435                 440                 445             


Glu Ser Gly Ala Arg Tyr Asp Glu Ala Val Leu Met Leu Gly Val Pro 
    450                 455                 460                 


His Lys Glu Lys Ser Ala Ile Leu Pro Pro Leu Asn Lys Thr Asp Ile 
465                 470                 475                 480 


Asp Ile Leu Asn Pro Thr Val Ile Arg Ala Phe Ala Gln Phe Arg Lys 
                485                 490                 495     


Val Ala Asn Ala Leu Val Arg Lys Tyr Gly Ala Phe Asp Arg Val His 
            500                 505                 510         


Phe Glu Leu Ala Arg Glu Ile Asn Thr Lys Gly Glu Ile Glu Asp Ile 
        515                 520                 525             


Lys Glu Ser Gln Arg Lys Asn Glu Lys Glu Arg Lys Glu Ala Ala Asp 
    530                 535                 540                 


Trp Ile Ala Glu Thr Ser Phe Gln Val Pro Leu Thr Arg Lys Asn Ile 
545                 550                 555                 560 


Leu Lys Lys Arg Leu Tyr Ile Gln Gln Asp Gly Arg Cys Ala Tyr Thr 
                565                 570                 575     


Gly Asp Val Ile Glu Leu Glu Arg Leu Phe Asp Glu Gly Tyr Cys Glu 
            580                 585                 590         


Ile Asp His Ile Leu Pro Arg Ser Arg Ser Ala Asp Asp Ser Phe Ala 
        595                 600                 605             


Asn Lys Val Leu Cys Leu Ala Arg Ala Asn Gln Gln Lys Thr Asp Arg 
    610                 615                 620                 


Thr Pro Tyr Glu Trp Phe Gly His Asp Ala Ala Arg Trp Asn Ala Phe 
625                 630                 635                 640 


Glu Thr Arg Thr Ser Ala Pro Ser Asn Arg Val Arg Thr Gly Lys Gly 
                645                 650                 655     


Lys Ile Asp Arg Leu Leu Lys Lys Asn Phe Asp Glu Asn Ser Glu Met 
            660                 665                 670         


Ala Phe Lys Asp Arg Asn Leu Asn Asp Thr Arg Tyr Met Ala Arg Ala 
        675                 680                 685             


Ile Lys Thr Tyr Cys Glu Gln Tyr Trp Val Phe Lys Asn Ser His Thr 
    690                 695                 700                 


Lys Ala Pro Val Gln Val Arg Ser Gly Lys Leu Thr Ser Val Leu Arg 
705                 710                 715                 720 


Tyr Gln Trp Gly Leu Glu Ser Lys Asp Arg Glu Ser His Thr His His 
                725                 730                 735     


Ala Val Asp Ala Ile Ile Ile Ala Phe Ser Thr Gln Gly Met Val Gln 
            740                 745                 750         


Lys Leu Ser Glu Tyr Tyr Arg Phe Lys Glu Thr His Arg Glu Lys Glu 
        755                 760                 765             


Arg Pro Lys Leu Ala Val Pro Leu Ala Asn Phe Arg Asp Ala Val Glu 
    770                 775                 780                 


Glu Ala Thr Arg Ile Glu Asn Thr Glu Thr Val Lys Glu Gly Val Glu 
785                 790                 795                 800 


Val Lys Arg Leu Leu Ile Ser Arg Pro Pro Arg Ala Arg Val Thr Gly 
                805                 810                 815     


Gln Ala His Glu Gln Thr Ala Lys Pro Tyr Pro Arg Ile Lys Gln Val 
            820                 825                 830         


Lys Asn Lys Lys Lys Trp Arg Leu Ala Pro Ile Asp Glu Glu Lys Phe 
        835                 840                 845             


Glu Ser Phe Lys Ala Asp Arg Val Ala Ser Ala Asn Gln Lys Asn Phe 
    850                 855                 860                 


Tyr Glu Thr Ser Thr Ile Pro Arg Val Asp Val Tyr His Lys Lys Gly 
865                 870                 875                 880 


Lys Phe His Leu Val Pro Ile Tyr Leu His Glu Met Val Leu Asn Glu 
                885                 890                 895     


Leu Pro Asn Leu Ser Leu Gly Thr Asn Pro Glu Ala Met Asp Glu Asn 
            900                 905                 910         


Phe Phe Lys Phe Ser Ile Phe Lys Asp Asp Leu Ile Ser Ile Gln Thr 
        915                 920                 925             


Gln Gly Thr Pro Lys Lys Pro Ala Lys Ile Ile Met Gly Tyr Phe Lys 
    930                 935                 940                 


Asn Met His Gly Ala Asn Met Val Leu Ser Ser Ile Asn Asn Ser Pro 
945                 950                 955                 960 


Cys Glu Gly Phe Thr Cys Thr Pro Val Ser Met Asp Lys Lys His Lys 
                965                 970                 975     


Asp Lys Cys Lys Leu Cys Pro Glu Glu Asn Arg Ile Ala Gly Arg Cys 
            980                 985                 990         


Leu Gln Gly Phe Leu Asp Tyr Trp  Ser Gln Glu Gly Leu  Arg Pro Pro 
        995                 1000                 1005             


Arg Lys  Glu Phe Glu Cys Asp  Gln Gly Val Lys Phe  Ala Leu Asp 
    1010                 1015                 1020             


Val Lys  Lys Tyr Gln Ile Asp  Pro Leu Gly Tyr Tyr  Tyr Glu Val 
    1025                 1030                 1035             


Lys Gln  Glu Lys Arg Leu Gly  Thr Ile Pro Gln Met  Arg Ser Ala 
    1040                 1045                 1050             


Lys Lys  Leu Val Lys Lys 
    1055                 


<210> 17
<211> 1082
<212> PRT
<213> Neisseria meningitidis

<400> 17
Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210> 18
<211> 1100
<212> PRT
<213> Actinomyces naeslundii

<400> 18
Met Trp Tyr Ala Ser Leu Met Ser Ala His His Leu Arg Val Gly Ile 
1               5                   10                  15      


Asp Val Gly Thr His Ser Val Gly Leu Ala Thr Leu Arg Val Asp Asp 
            20                  25                  30          


His Gly Thr Pro Ile Glu Leu Leu Ser Ala Leu Ser His Ile His Asp 
        35                  40                  45              


Ser Gly Val Gly Lys Glu Gly Lys Lys Asp His Asp Thr Arg Lys Lys 
    50                  55                  60                  


Leu Ser Gly Ile Ala Arg Arg Ala Arg Arg Leu Leu His His Arg Arg 
65                  70                  75                  80  


Thr Gln Leu Gln Gln Leu Asp Glu Val Leu Arg Asp Leu Gly Phe Pro 
                85                  90                  95      


Ile Pro Thr Pro Gly Glu Phe Leu Asp Leu Asn Glu Gln Thr Asp Pro 
            100                 105                 110         


Tyr Arg Val Trp Arg Val Arg Ala Arg Leu Val Glu Glu Lys Leu Pro 
        115                 120                 125             


Glu Glu Leu Arg Gly Pro Ala Ile Ser Met Ala Val Arg His Ile Ala 
    130                 135                 140                 


Arg His Arg Gly Trp Arg Asn Pro Tyr Ser Lys Val Glu Ser Leu Leu 
145                 150                 155                 160 


Ser Pro Ala Glu Glu Ser Pro Phe Met Lys Ala Leu Arg Glu Arg Ile 
                165                 170                 175     


Leu Ala Thr Thr Gly Glu Val Leu Asp Asp Gly Ile Thr Pro Gly Gln 
            180                 185                 190         


Ala Met Ala Gln Val Ala Leu Thr His Asn Ile Ser Met Arg Gly Pro 
        195                 200                 205             


Glu Gly Ile Leu Gly Lys Leu His Gln Ser Asp Asn Ala Asn Glu Ile 
    210                 215                 220                 


Arg Lys Ile Cys Ala Arg Gln Gly Val Ser Pro Asp Val Cys Lys Gln 
225                 230                 235                 240 


Leu Leu Arg Ala Val Phe Lys Ala Asp Ser Pro Arg Gly Ser Ala Val 
                245                 250                 255     


Ser Arg Val Ala Pro Asp Pro Leu Pro Gly Gln Gly Ser Phe Arg Arg 
            260                 265                 270         


Ala Pro Lys Cys Asp Pro Glu Phe Gln Arg Phe Arg Ile Ile Ser Ile 
        275                 280                 285             


Val Ala Asn Leu Arg Ile Ser Glu Thr Lys Gly Glu Asn Arg Pro Leu 
    290                 295                 300                 


Thr Ala Asp Glu Arg Arg His Val Val Thr Phe Leu Thr Glu Asp Ser 
305                 310                 315                 320 


Gln Ala Asp Leu Thr Trp Val Asp Val Ala Glu Lys Leu Gly Val His 
                325                 330                 335     


Arg Arg Asp Leu Arg Gly Thr Ala Val His Thr Asp Asp Gly Glu Arg 
            340                 345                 350         


Ser Ala Ala Arg Pro Pro Ile Asp Ala Thr Asp Arg Ile Met Arg Gln 
        355                 360                 365             


Thr Lys Ile Ser Ser Leu Lys Thr Trp Trp Glu Glu Ala Asp Ser Glu 
    370                 375                 380                 


Gln Arg Gly Ala Met Ile Arg Tyr Leu Tyr Glu Asp Pro Thr Asp Ser 
385                 390                 395                 400 


Glu Cys Ala Glu Ile Ile Ala Glu Leu Pro Glu Glu Asp Gln Ala Lys 
                405                 410                 415     


Leu Asp Ser Leu His Leu Pro Ala Gly Arg Ala Ala Tyr Ser Glu Ser 
            420                 425                 430         


Leu Thr Ala Leu Ser Asp His Met Leu Ala Thr Thr Asp Asp Leu His 
        435                 440                 445             


Glu Ala Arg Lys Arg Leu Phe Gly Val Asp Asp Ser Trp Ala Pro Pro 
    450                 455                 460                 


Ala Glu Ala Ile Asn Ala Pro Val Gly Asn Pro Ser Val Asp Arg Thr 
465                 470                 475                 480 


Leu Lys Ile Val Gly Arg Tyr Leu Ser Ala Val Glu Ser Met Trp Gly 
                485                 490                 495     


Thr Pro Glu Val Ile His Val Glu His Val Arg Asp Gly Phe Thr Ser 
            500                 505                 510         


Glu Arg Met Ala Asp Glu Arg Asp Lys Ala Asn Arg Arg Arg Tyr Asn 
        515                 520                 525             


Asp Asn Gln Glu Ala Met Lys Lys Ile Gln Arg Asp Tyr Gly Lys Glu 
    530                 535                 540                 


Gly Tyr Ile Ser Arg Gly Asp Ile Val Arg Leu Asp Ala Leu Glu Leu 
545                 550                 555                 560 


Gln Gly Cys Ala Cys Leu Tyr Cys Gly Thr Thr Ile Gly Tyr His Thr 
                565                 570                 575     


Cys Gln Leu Asp His Ile Val Pro Gln Ala Gly Pro Gly Ser Asn Asn 
            580                 585                 590         


Arg Arg Gly Asn Leu Val Ala Val Cys Glu Arg Cys Asn Arg Ser Lys 
        595                 600                 605             


Ser Asn Thr Pro Phe Ala Val Trp Ala Gln Lys Cys Gly Ile Pro His 
    610                 615                 620                 


Val Gly Val Lys Glu Ala Ile Gly Arg Val Arg Gly Trp Arg Lys Gln 
625                 630                 635                 640 


Thr Pro Asn Thr Ser Ser Glu Asp Leu Thr Arg Leu Lys Lys Glu Val 
                645                 650                 655     


Ile Ala Arg Leu Arg Arg Thr Gln Glu Asp Pro Glu Ile Asp Glu Arg 
            660                 665                 670         


Ser Met Glu Ser Val Ala Trp Met Ala Asn Glu Leu His His Arg Ile 
        675                 680                 685             


Ala Ala Ala Tyr Pro Glu Thr Thr Val Met Val Tyr Arg Gly Ser Ile 
    690                 695                 700                 


Thr Ala Ala Ala Arg Lys Ala Ala Gly Ile Asp Ser Arg Ile Asn Leu 
705                 710                 715                 720 


Ile Gly Glu Lys Gly Arg Lys Asp Arg Ile Asp Arg Arg His His Ala 
                725                 730                 735     


Val Asp Ala Ser Val Val Ala Leu Met Glu Ala Ser Val Ala Lys Thr 
            740                 745                 750         


Leu Ala Glu Arg Ser Ser Leu Arg Gly Glu Gln Arg Leu Thr Gly Lys 
        755                 760                 765             


Glu Gln Thr Trp Lys Gln Tyr Thr Gly Ser Thr Val Gly Ala Arg Glu 
    770                 775                 780                 


His Phe Glu Met Trp Arg Gly His Met Leu His Leu Thr Glu Leu Phe 
785                 790                 795                 800 


Asn Glu Arg Leu Ala Glu Asp Lys Val Tyr Val Thr Gln Asn Ile Arg 
                805                 810                 815     


Leu Arg Leu Ser Asp Gly Asn Ala His Thr Val Asn Pro Ser Lys Leu 
            820                 825                 830         


Val Ser His Arg Leu Gly Asp Gly Leu Thr Val Gln Gln Ile Asp Arg 
        835                 840                 845             


Ala Cys Thr Pro Ala Leu Trp Cys Ala Leu Thr Arg Glu Lys Asp Phe 
    850                 855                 860                 


Asp Glu Lys Asn Gly Leu Pro Ala Arg Glu Asp Arg Ala Ile Arg Val 
865                 870                 875                 880 


His Gly His Glu Ile Lys Ser Ser Asp Tyr Ile Gln Val Phe Ser Lys 
                885                 890                 895     


Arg Lys Lys Thr Asp Ser Asp Arg Asp Glu Thr Pro Phe Gly Ala Ile 
            900                 905                 910         


Ala Val Arg Gly Gly Phe Val Glu Ile Gly Pro Ser Ile His His Ala 
        915                 920                 925             


Arg Ile Tyr Arg Val Glu Gly Lys Lys Pro Val Tyr Ala Met Leu Arg 
    930                 935                 940                 


Val Phe Thr His Asp Leu Leu Ser Gln Arg His Gly Asp Leu Phe Ser 
945                 950                 955                 960 


Ala Val Ile Pro Pro Gln Ser Ile Ser Met Arg Cys Ala Glu Pro Lys 
                965                 970                 975     


Leu Arg Lys Ala Ile Thr Thr Gly Asn Ala Thr Tyr Leu Gly Trp Val 
            980                 985                 990         


Val Val Gly Asp Glu Leu Glu Ile  Asn Val Asp Ser Phe  Thr Lys Tyr 
        995                 1000                 1005             


Ala Ile  Gly Arg Phe Leu Glu  Asp Phe Pro Asn Thr  Thr Arg Trp 
    1010                 1015                 1020             


Arg Ile  Cys Gly Tyr Asp Thr  Asn Ser Lys Leu Thr  Leu Lys Pro 
    1025                 1030                 1035             


Ile Val  Leu Ala Ala Glu Gly  Leu Glu Asn Pro Ser  Ser Ala Val 
    1040                 1045                 1050             


Asn Glu  Ile Val Glu Leu Lys  Gly Trp Arg Val Ala  Ile Asn Val 
    1055                 1060                 1065             


Leu Thr  Lys Val His Pro Thr  Val Val Arg Arg Asp  Ala Leu Gly 
    1070                 1075                 1080             


Arg Pro  Arg Tyr Ser Ser Arg  Ser Asn Leu Pro Thr  Ser Trp Thr 
    1085                 1090                 1095             


Ile Glu  
    1100 


<210> 19
<211> 1087
<212> PRT
<213> Geobacillus stearothermophilus

<400> 19
Met Arg Tyr Lys Ile Gly Leu Asp Ile Gly Ile Thr Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Met Asn Leu Asp Ile Pro Arg Ile Glu Asp Leu Gly Val Arg 
            20                  25                  30          


Ile Phe Asp Arg Ala Glu Asn Pro Gln Thr Gly Glu Ser Leu Ala Leu 
        35                  40                  45              


Pro Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Leu Arg Arg Arg Lys 
    50                  55                  60                  


His Arg Leu Glu Arg Ile Arg Arg Leu Val Ile Arg Glu Gly Ile Leu 
65                  70                  75                  80  


Thr Lys Glu Glu Leu Asp Lys Leu Phe Glu Glu Lys His Glu Ile Asp 
                85                  90                  95      


Val Trp Gln Leu Arg Val Glu Ala Leu Asp Arg Lys Leu Asn Asn Asp 
            100                 105                 110         


Glu Leu Ala Arg Val Leu Leu His Leu Ala Lys Arg Arg Gly Phe Lys 
        115                 120                 125             


Ser Asn Arg Lys Ser Glu Arg Ser Asn Lys Glu Asn Ser Thr Met Leu 
    130                 135                 140                 


Lys His Ile Glu Glu Asn Arg Ala Ile Leu Ser Ser Tyr Arg Thr Val 
145                 150                 155                 160 


Gly Glu Met Ile Val Lys Asp Pro Lys Phe Ala Leu His Lys Arg Asn 
                165                 170                 175     


Lys Gly Glu Asn Tyr Thr Asn Thr Ile Ala Arg Asp Asp Leu Glu Arg 
            180                 185                 190         


Glu Ile Arg Leu Ile Phe Ser Lys Gln Arg Glu Phe Gly Asn Met Ser 
        195                 200                 205             


Cys Thr Glu Glu Phe Glu Asn Glu Tyr Ile Thr Ile Trp Ala Ser Gln 
    210                 215                 220                 


Arg Pro Val Ala Ser Lys Asp Asp Ile Glu Lys Lys Val Gly Phe Cys 
225                 230                 235                 240 


Thr Phe Glu Pro Lys Glu Lys Arg Ala Pro Lys Ala Thr Tyr Thr Phe 
                245                 250                 255     


Gln Ser Phe Ile Ala Trp Glu His Ile Asn Lys Leu Arg Leu Ile Ser 
            260                 265                 270         


Pro Ser Gly Ala Arg Gly Leu Thr Asp Glu Glu Arg Arg Leu Leu Tyr 
        275                 280                 285             


Glu Gln Ala Phe Gln Lys Asn Lys Ile Thr Tyr His Asp Ile Arg Thr 
    290                 295                 300                 


Leu Leu His Leu Pro Asp Asp Thr Tyr Phe Lys Gly Ile Val Tyr Asp 
305                 310                 315                 320 


Arg Gly Glu Ser Arg Lys Gln Asn Glu Asn Ile Arg Phe Leu Glu Leu 
                325                 330                 335     


Asp Ala Tyr His Gln Ile Arg Lys Ala Val Asp Lys Val Tyr Gly Lys 
            340                 345                 350         


Gly Lys Ser Ser Ser Phe Leu Pro Ile Asp Phe Asp Thr Phe Gly Tyr 
        355                 360                 365             


Ala Leu Thr Leu Phe Lys Asp Asp Ala Asp Ile His Ser Tyr Leu Arg 
    370                 375                 380                 


Asn Glu Tyr Glu Gln Asn Gly Lys Arg Met Pro Asn Leu Ala Asn Lys 
385                 390                 395                 400 


Val Tyr Asp Asn Glu Leu Ile Glu Glu Leu Leu Asn Leu Ser Phe Thr 
                405                 410                 415     


Lys Phe Gly His Leu Ser Leu Lys Ala Leu Arg Ser Ile Leu Pro Tyr 
            420                 425                 430         


Met Glu Gln Gly Glu Val Tyr Ser Ser Ala Cys Glu Arg Ala Gly Tyr 
        435                 440                 445             


Thr Phe Thr Gly Pro Lys Lys Lys Gln Lys Thr Met Leu Leu Pro Asn 
    450                 455                 460                 


Ile Pro Pro Ile Ala Asn Pro Val Val Met Arg Ala Leu Thr Gln Ala 
465                 470                 475                 480 


Arg Lys Val Val Asn Ala Ile Ile Lys Lys Tyr Gly Ser Pro Val Ser 
                485                 490                 495     


Ile His Ile Glu Leu Ala Arg Asp Leu Ser Gln Thr Phe Asp Glu Arg 
            500                 505                 510         


Arg Lys Thr Lys Lys Glu Gln Asp Glu Asn Arg Lys Lys Asn Glu Thr 
        515                 520                 525             


Ala Ile Arg Gln Leu Met Glu Tyr Gly Leu Thr Leu Asn Pro Thr Gly 
    530                 535                 540                 


His Asp Ile Val Lys Phe Lys Leu Trp Ser Glu Gln Asn Gly Arg Cys 
545                 550                 555                 560 


Ala Tyr Ser Leu Gln Pro Ile Glu Ile Glu Arg Leu Leu Glu Pro Gly 
                565                 570                 575     


Tyr Val Glu Val Asp His Val Ile Pro Tyr Ser Arg Ser Leu Asp Asp 
            580                 585                 590         


Ser Tyr Thr Asn Lys Val Leu Val Leu Thr Arg Glu Asn Arg Glu Lys 
        595                 600                 605             


Gly Asn Arg Ile Pro Ala Glu Tyr Leu Gly Val Gly Thr Glu Arg Trp 
    610                 615                 620                 


Gln Gln Phe Glu Thr Phe Val Leu Thr Asn Lys Gln Phe Ser Lys Lys 
625                 630                 635                 640 


Lys Arg Asp Arg Leu Leu Arg Leu His Tyr Asp Glu Asn Glu Glu Thr 
                645                 650                 655     


Glu Phe Lys Asn Arg Asn Leu Asn Asp Thr Arg Tyr Ile Ser Arg Phe 
            660                 665                 670         


Phe Ala Asn Phe Ile Arg Glu His Leu Lys Phe Ala Glu Ser Asp Asp 
        675                 680                 685             


Lys Gln Lys Val Tyr Thr Val Asn Gly Arg Val Thr Ala His Leu Arg 
    690                 695                 700                 


Ser Arg Trp Glu Phe Asn Lys Asn Arg Glu Glu Ser Asp Leu His His 
705                 710                 715                 720 


Ala Val Asp Ala Ala Ile Val Ala Cys Thr Thr Pro Ser Asp Ile Ala 
                725                 730                 735     


Lys Val Thr Ala Phe Tyr Gln Arg Arg Glu Gln Asn Lys Glu Leu Ala 
            740                 745                 750         


Lys Lys Thr Glu Pro His Phe Pro Gln Pro Trp Pro His Phe Ala Asp 
        755                 760                 765             


Glu Leu Arg Ala Arg Leu Ser Lys His Pro Lys Glu Ser Ile Lys Ala 
    770                 775                 780                 


Leu Asn Leu Gly Asn Tyr Asp Asp Gln Lys Leu Glu Ser Leu Gln Pro 
785                 790                 795                 800 


Val Phe Val Ser Arg Met Pro Lys Arg Ser Val Thr Gly Ala Ala His 
                805                 810                 815     


Gln Glu Thr Leu Arg Arg Tyr Val Gly Ile Asp Glu Arg Ser Gly Lys 
            820                 825                 830         


Ile Gln Thr Val Val Lys Thr Lys Leu Ser Glu Ile Lys Leu Asp Ala 
        835                 840                 845             


Ser Gly His Phe Pro Met Tyr Gly Lys Glu Ser Asp Pro Arg Thr Tyr 
    850                 855                 860                 


Glu Ala Ile Arg Gln Arg Leu Leu Glu His Asn Asn Asp Pro Lys Lys 
865                 870                 875                 880 


Ala Phe Gln Glu Pro Leu Tyr Lys Pro Lys Lys Asn Gly Glu Pro Gly 
                885                 890                 895     


Pro Val Ile Arg Thr Val Lys Ile Ile Asp Thr Lys Asn Gln Val Ile 
            900                 905                 910         


Pro Leu Asn Asp Gly Lys Thr Val Ala Tyr Asn Ser Asn Ile Val Arg 
        915                 920                 925             


Val Asp Val Phe Glu Lys Asp Gly Lys Tyr Tyr Cys Val Pro Val Tyr 
    930                 935                 940                 


Thr Met Asp Ile Met Lys Gly Ile Leu Pro Asn Lys Ala Ile Glu Pro 
945                 950                 955                 960 


Asn Lys Pro Tyr Ser Glu Trp Lys Glu Met Thr Glu Asp Tyr Thr Phe 
                965                 970                 975     


Arg Phe Ser Leu Tyr Pro Asn Asp Leu Ile Arg Ile Glu Leu Pro Arg 
            980                 985                 990         


Glu Lys Thr Val Lys Thr Ala Ala  Gly Glu Glu Ile Asn  Val Lys Asp 
        995                 1000                 1005             


Val Phe  Val Tyr Tyr Lys Thr  Ile Asp Ser Ala Asn  Gly Gly Leu 
    1010                 1015                 1020             


Glu Leu  Ile Ser His Asp His  Arg Phe Ser Leu Arg  Gly Val Gly 
    1025                 1030                 1035             


Ser Arg  Thr Leu Lys Arg Phe  Glu Lys Tyr Gln Val  Asp Val Leu 
    1040                 1045                 1050             


Gly Asn  Ile Tyr Lys Val Arg  Gly Glu Lys Arg Val  Gly Leu Ala 
    1055                 1060                 1065             


Ser Ser  Ala His Ser Lys Thr  Gly Glu Thr Val Arg  Pro Leu Gln 
    1070                 1075                 1080             


Ser Thr  Arg Asp 
    1085         


<210> 20
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 20
His His His His His His 
1               5       

