﻿               SEQUENCE LISTING

<110> UNIVERSITA’ DEGLI STUDI DI SIENA; ISTITUTO PER LO STUDIO, LA PREVENZIONE E LA RETE ONCOLOGICA (ISPRO); UNIVERSITA’ DEGLI STUDI DI FIRENZE

<120> CRISPR system for genome editing

<130> PC1711RRI

<160> 8

<170> BiSSAP 1.3.6

<210> 1
<211> 13710
<212> DNA
<213> Artificial sequence

<220> 
<223> CRISPR-Cas viral vector

<400> 1
caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat       60

taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac      120

tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc      180

ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga      240

gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca      300

cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat      360

accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc      420

cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc      480

ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa      540

ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag      600

atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta      660

gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac      720

agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg      780

ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta      840

caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt      900

tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaagtcgac      960

gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa     1020

gaggccgcag agccggcagc aggccctaga gagggcctat ttcccatgat tccttcatat     1080

ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca     1140

aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt     1200

ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat     1260

ttcttggctt tatatatctt gtggaaagga cgaaacaccg taatttctac tcttgtagat     1320

tctgctaatc ctgttaccag ccctaatttc tactcttgta gattcagcag cgctcatggt     1380

gggggctttt ttctattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg     1440

cgatgtacgg gccagatata cgcgggtacc ctctagaact agtggatcat ttgtcgacga     1500

attctatctc tgctaatcct gttaccagcc cggatccatg gaaagaagaa gcgcaaggtg     1560

ggaagcggag ctactaactt cagcctgctg aagcaggctg gcgacgtgga ggagaaccct     1620

ggacctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag     1680

ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc     1740

acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg     1800

cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac     1860

atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc     1920

atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac     1980

accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg     2040

gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag     2100

aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag     2160

ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac     2220

aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac     2280

atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac     2340

aagtactcag atctcgagct caagcttgag ggcagaggaa gccttctaac atgcggggac     2400

gtggaggaaa atcccggccc catggcttcg tacccctgcc atcaacacgc gtctgcgttc     2460

gaccaggctg cgcgttctcg cggccataac aaccgacgta cggcgttgcg ccctcgccgg     2520

cagcaaaaag ccacggaagt ccgcctggag cagaaaatgc ccacgctact gcgggtttat     2580

atagacggtc cccacgggat ggggaaaacc accaccacgc aactgctggt ggccctgggt     2640

tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcgggt gttgggggct     2700

tccgagacaa tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg tgagatatcg     2760

gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat gccttatgcc     2820

gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc acatgccccg     2880

cccccggccc tcaccctcat cttcgatagg catcccatcg ccgccctcct gtgctacccg     2940

gccgcgcgat accttatggg cagcatgacc ccccaggccg tgctggcgtt cgtggccctc     3000

atcccgccga ccttgcccgg cacaaacatc gtgttggggg cccttccgga ggacagacac     3060

atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc tatgctggcc     3120

gcgattcgcc gcgtttatgg gctgcttgcc aatacggtgc ggtatctgca gggcggcggg     3180

tcgtggcggg aggattgggg acagctttcg ggggcggccg tgccgcccca gggtgccgag     3240

ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac cctgtttcgg     3300

gcccccgagt tgctggcccc caacggcgac ctgtataacg tgtttgcctg ggctttggac     3360

gtcttggcca aacgcctccg tcccatgcac gtctttatcc tggattacga ccaatcgccc     3420

gccggctgcc gggacgccct gctgcaactt acctccggga tggtccagac ccacgtcacc     3480

accccaggct ccataccgac gatctgcgac ctggcgcgca cgtttgcccg ggagatgggg     3540

gaggctaact aaagcgtctc tgtcgtgccc ccaccatgag cgctgctgag atatctgcga     3600

gacggtcgac tttagatcca agcttatcga taccggtacc cgttacataa cttacggtaa     3660

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa     3720

tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag     3780

tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc     3840

ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct     3900

acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc     3960

ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg     4020

cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg     4080

ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa     4140

agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc     4200

gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc     4260

gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt     4320

ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg     4380

gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac     4440

cggctagcgt ttaaacttaa gcttgccacc atggccccaa agaagaagcg gaaggtcggt     4500

atccacggag tcccagcagc cacacagttc gagggcttta ccaacctgta tcaggtgagc     4560

aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat ccaggagcag     4620

ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa gcccatcatc     4680

gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct ggattgggag     4740

aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac aaggaacgcc     4800

ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat cggccggaca     4860

gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg cctgttcaag     4920

gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac aaccgagcac     4980

gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg cttttatgag     5040

aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca ccgcatcgtg     5100

caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct gatcaccgcc     5160

gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat cttcgtgagc     5220

acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac acagacccag     5280

atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac cgagaagatc     5340

aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac agcccacatc     5400

atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc cgataggaac     5460

accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca gtccttctgc     5520

aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc cctgtttaac     5580

gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct ggagacaatc     5640

agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga gcggagaatc     5700

tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg cagcctgaag     5760

cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct gagcgaggcc     5820

ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga tcagccactg     5880

cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct ggacagcctg     5940

ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga ggtggacccc     6000

gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct gagcttctac     6060

aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt caagctgaac     6120

tttcagatgc ctacactggc ccggggctgg gacgtgaatg tggagaagaa ccggggcgcc     6180

atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca gaagggcagg     6240

tataaggccc tcagcttcga gcccacagag aaaaccagcg agggctttga taagatgtac     6300

tatgactact ttccggatgc cgccaagatg atcccaaagt gcagcaccca gctgaaggcg     6360

gtgaccgccc actttcagac ccacacaacc cccatcctgc tgtccaacaa tttcatcgag     6420

cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga gccaaagaag     6480

tttcagacag cgtacgccaa gaaaaccggc gaccagaagg gctacagaga ggccctgtgc     6540

aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac ctctatcgat     6600

ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta tgccgagctg     6660

aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat catggatgcc     6720

gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc caagggccac     6780

cacggcaagc ctaatctgca cacactgtat tggaccggtc tgttttctcc agagaacctg     6840

gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc taagtccagg     6900

atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct gaaggatcag     6960

aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa tcacagactg     7020

tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac caaggaggtg     7080

tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt ccacgtgcct     7140

atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag ggtgaatgcc     7200

tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga gagaaacctg     7260

atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag cctgaacacc     7320

atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag ggtggcagca     7380

aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta tctgagccag     7440

gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt gctggagaac     7500

ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt gtaccagcag     7560

ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta tccagcagag     7620

aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc ctttgccaag     7680

atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc taagatcgat     7740

cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca cgagagccgc     7800

aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg cgacttcatc     7860

ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg ctttatgcct     7920

gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg cacccctttc     7980

atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg cagataccgg     8040

gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat cgtgttcagg     8100

gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc catcgacacc     8160

atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc cacaggcgag     8220

gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc ccggtttcag     8280

aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc cctgaagggc     8340

cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa cggcatctcc     8400

aatcaggact ggctggccta catccaggag ctgcgcaaca aaaggccggc ggccacgaaa     8460

aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc agattacgct     8520

tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta tgcctaagaa     8580

ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt     8640

gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     8700

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     8760

tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc     8820

cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag     8880

cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat     8940

tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta     9000

tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct     9060

ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc     9120

gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat     9180

ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct     9240

cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt     9300

tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta     9360

aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg     9420

aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag     9480

ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac gcgctgctta     9540

tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac     9600

gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg     9660

ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc     9720

aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa     9780

agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt     9840

caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc     9900

gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat     9960

acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta    10020

ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg    10080

attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc    10140

gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt    10200

gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag    10260

ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata    10320

ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg    10380

cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca    10440

ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt    10500

ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga    10560

aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga    10620

actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat    10680

gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca    10740

agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt    10800

cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac    10860

catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct    10920

aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga    10980

gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac    11040

aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat    11100

agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg    11160

ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc    11220

actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc    11280

aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg    11340

gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta    11400

atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg    11460

tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga    11520

tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt    11580

ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag    11640

agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa    11700

ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag    11760

tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca    11820

gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac    11880

cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa    11940

ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc    12000

agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg    12060

tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc    12120

ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc    12180

ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag    12240

ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa    12300

accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga    12360

ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc    12420

ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca    12480

atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg    12540

tatacagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga    12600

aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc    12660

tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat    12720

catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt    12780

ggaatcgaaa tctcgtagca cgtgtcagtc ctgctcctcg gccacgaagt gcacgcagtt    12840

gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat    12900

ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag    12960

ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg    13020

gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac    13080

gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg    13140

cgcggtgagc accggaacgg cactggtcaa cttggccatg gtttagttcc tcaccttgtc    13200

gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgatcagat    13260

ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag    13320

cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata    13380

aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg    13440

atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata    13500

cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc    13560

atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac    13620

acattccaca gaattaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat    13680

aggccgaaat cggcaaaatc ccttataaat                                     13710


<210> 2
<211> 43
<212> DNA
<213> Artificial sequence


<220> 
<223> Sequence encoding the first gRNA

<400> 2
taatttctac tcttgtagat tcagcagcgc tcatggtggg ggc                       43


<210> 3
<211> 43
<212> DNA
<213> Artificial sequence


<220> 
<223> Sequence encoding the second gRNA

<400> 3
taatttctac tcttgtagat tctgctaatc ctgttaccag ccc                       43


<210> 4
<211> 2035
<212> DNA
<213> Artificial sequence


<220> 
<223> EGFP-HSV-TK cassette

<400> 4
ggatccatgg aaagaagaag cgcaaggtgg gaagcggagc tactaacttc agcctgctga      60

agcaggctgg cgacgtggag gagaaccctg gacctatggt gagcaagggc gaggagctgt     120

tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca     180

gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct     240

gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg     300

tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca     360

tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga     420

cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca     480

tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc     540

acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc     600

gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca     660

tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga     720

gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg     780

ggatcactct cggcatggac gagctgtaca agtactcaga tctcgagctc aagcttgagg     840

gcagaggaag ccttctaaca tgcggggacg tggaggaaaa tcccggcccc atggcttcgt     900

acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc ggccataaca     960

accgacgtac ggcgttgcgc cctcgccggc agcaaaaagc cacggaagtc cgcctggagc    1020

agaaaatgcc cacgctactg cgggtttata tagacggtcc ccacgggatg gggaaaacca    1080

ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac gtacccgagc    1140

cgatgactta ctggcgggtg ttgggggctt ccgagacaat cgcgaacatc tacaccacac    1200

aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta atgacaagcg    1260

cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg    1320

ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc ttcgataggc    1380

atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc    1440

cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg    1500

tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc cagcgccccg    1560

gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttatggg ctgcttgcca    1620

atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga cagctttcgg    1680

gggcggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata    1740

tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc    1800

tgtataacgt gtttgcctgg gctttggacg tcttggccaa acgcctccgt cccatgcacg    1860

tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta    1920

cctccgggat ggtccagacc cacgtcacca ccccaggctc cataccgacg atctgcgacc    1980

tggcgcgcac gtttgcccgg gagatggggg aggctaacta aagcgtctct gtcgt         2035


<210> 5
<211> 4107
<212> DNA
<213> Artificial sequence


<220> 
<223> Sequence encoding Cas12a

<400> 5
atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cacacagttc       60

gagggcttta ccaacctgta tcaggtgagc aagacactgc ggtttgagct gatcccacag      120

ggcaagaccc tgaagcacat ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat      180

gatcactaca aggagctgaa gcccatcatc gatcggatct acaagaccta tgccgaccag      240

tgcctgcagc tggtgcagct ggattgggag aacctgagcg ccgccatcga ctcctataga      300

aaggagaaaa ccgaggagac aaggaacgcc ctgatcgagg agcaggccac atatcgcaat      360

gccatccacg actacttcat cggccggaca gacaacctga ccgatgccat caataagaga      420

cacgccgaga tctacaaggg cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag      480

cagctgggca ccgtgaccac aaccgagcac gagaacgccc tgctgcggag cttcgacaag      540

tttacaacct acttctccgg cttttatgag aacaggaaga acgtgttcag cgccgaggat      600

atcagcacag ccatcccaca ccgcatcgtg caggacaact tccccaagtt taaggagaat      660

tgtcacatct tcacacgcct gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac      720

gtgaagaagg ccatcggcat cttcgtgagc acctccatcg aggaggtgtt ttccttccct      780

ttttataacc agctgctgac acagacccag atcgacctgt ataaccagct gctgggagga      840

atctctcggg aggcaggcac cgagaagatc aagggcctga acgaggtgct gaatctggcc      900

atccagaaga atgatgagac agcccacatc atcgcctccc tgccacacag attcatcccc      960

ctgtttaagc agatcctgtc cgataggaac accctgtctt tcatcctgga ggagtttaag     1020

agcgacgagg aagtgatcca gtccttctgc aagtacaaga cactgctgag aaacgagaac     1080

gtgctggaga cagccgaggc cctgtttaac gagctgaaca gcatcgacct gacacacatc     1140

ttcatcagcc acaagaagct ggagacaatc agcagcgccc tgtgcgacca ctgggataca     1200

ctgaggaatg ccctgtatga gcggagaatc tccgagctga caggcaagat caccaagtct     1260

gccaaggaga aggtgcagcg cagcctgaag cacgaggata tcaacctgca ggagatcatc     1320

tctgccgcag gcaaggagct gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc     1380

cacgcacacg ccgccctgga tcagccactg cctacaaccc tgaagaagca ggaggagaag     1440

gagatcctga agtctcagct ggacagcctg ctgggcctgt accacctgct ggactggttt     1500

gccgtggatg agtccaacga ggtggacccc gagttctctg cccggctgac cggcatcaag     1560

ctggagatgg agccttctct gagcttctac aacaaggcca gaaattatgc caccaagaag     1620

ccctactccg tggagaagtt caagctgaac tttcagatgc ctacactggc ccggggctgg     1680

gacgtgaatg tggagaagaa ccggggcgcc atcctgtttg tgaagaacgg cctgtactat     1740

ctgggcatca tgccaaagca gaagggcagg tataaggccc tcagcttcga gcccacagag     1800

aaaaccagcg agggctttga taagatgtac tatgactact ttccggatgc cgccaagatg     1860

atcccaaagt gcagcaccca gctgaaggcg gtgaccgccc actttcagac ccacacaacc     1920

cccatcctgc tgtccaacaa tttcatcgag cctctggaga tcacaaagga gatctacgac     1980

ctgaacaatc ctgagaagga gccaaagaag tttcagacag cgtacgccaa gaaaaccggc     2040

gaccagaagg gctacagaga ggccctgtgc aagtggatcg acttcacaag ggattttctg     2100

tccaagtata ccaagacaac ctctatcgat ctgtctagcc tgcggccatc ctctcagtat     2160

aaggacctgg gcgagtacta tgccgagctg aatcccctgc tgtaccacat cagcttccag     2220

agaatcgccg agaaggagat catggatgcc gtggagacag gcaagctgta cctgttccag     2280

atctataaca aggactttgc caagggccac cacggcaagc ctaatctgca cacactgtat     2340

tggaccggtc tgttttctcc agagaacctg gccaagacaa gcatcaagct gaatggccag     2400

gccgagctgt tctaccgccc taagtccagg atgaagagga tggcacaccg gctgggagag     2460

aagatgctga acaagaagct gaaggatcag aaaaccccaa tccccgacac cctgtaccag     2520

gagctgtacg actatgtgaa tcacagactg tcccacgacc tgtctgatga ggccagggcc     2580

ctgctgccca acgtgatcac caaggaggtg tctcacgaga tcatcaagga taggcgcttt     2640

accagcgaca agttcttttt ccacgtgcct atcacactga actatcaggc cgccaattcc     2700

ccatctaagt tcaaccagag ggtgaatgcc tacctgaagg agcaccccga gacacctatc     2760

atcggcatcg atcggggcga gagaaacctg atctatatca cagtgatcga ctccaccggc     2820

aagatcctgg agcagcggag cctgaacacc atccagcagt ttgattacca gaagaagctg     2880

gacaacaggg agaaggagag ggtggcagca aggcaggcct ggtctgtggt gggcacaatc     2940

aaggatctga agcagggcta tctgagccag gtcatccacg agatcgtgga cctgatgatc     3000

cactaccagg ccgtggtggt gctggagaac ctgaatttcg gctttaagag caagaggacc     3060

ggcatcgccg agaaggccgt gtaccagcag ttcgagaaga tgctgatcga taagctgaat     3120

tgcctggtgc tgaaggacta tccagcagag aaagtgggag gcgtgctgaa cccataccag     3180

ctgacagacc agttcacctc ctttgccaag atgggcaccc agtctggctt cctgttttac     3240

gtgcctgccc catatacatc taagatcgat cccctgaccg gcttcgtgga ccccttcgtg     3300

tggaaaacca tcaagaatca cgagagccgc aagcacttcc tggagggctt cgactttctg     3360

cactacgacg tgaaaaccgg cgacttcatc ctgcacttta agatgaacag aaatctgtcc     3420

ttccagaggg gcctgcccgg ctttatgcct gcatgggata tcgtgttcga gaagaacgag     3480

acacagtttg acgccaaggg cacccctttc atcgccggca agagaatcgt gccagtgatc     3540

gagaatcaca gattcaccgg cagataccgg gacctgtatc ctgccaacga gctgatcgcc     3600

ctgctggagg agaagggcat cgtgttcagg gatggctcca acatcctgcc aaagctgctg     3660

gagaatgacg attctcacgc catcgacacc atggtggccc tgatccgcag cgtgctgcag     3720

atgcggaact ccaatgccgc cacaggcgag gactatatca acagccccgt gcgcgatctg     3780

aatggcgtgt gcttcgactc ccggtttcag aacccagagt ggcccatgga cgccgatgcc     3840

aatggcgcct accacatcgc cctgaagggc cagctgctgc tgaatcacct gaaggagagc     3900

aaggatctga agctgcagaa cggcatctcc aatcaggact ggctggccta catccaggag     3960

ctgcgcaaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga     4020

tcctacccat acgatgttcc agattacgct tatccctacg acgtgcctga ttatgcatac     4080

ccatatgatg tccccgacta tgcctaa                                         4107


<210> 6
<211> 23
<212> DNA
<213> Artificial sequence


<220> 
<223> First target sequence

<400> 6
gcccccacca tgagcgctgc tga                                            23


<210> 7
<211> 23
<212> DNA
<213> Artificial sequence


<220> 
<223> Second target sequence 

<400> 7
tctgctaatc ctgttaccag ccc                                            23


<210> 8
<211> 249
<212> DNA
<213> Artificial sequence


<220> 
<223> U6 promoter

<400> 8
gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag     60

ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga    120

aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat    180

atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga    240

cgaaacacc                                                            249


