               SEQUENCE LISTING

<110> Universiteit Antwerpen

<120> Anti-pathogenic activity of a bifunctional peptidoglycan/chitin hydrolase

<130> UNA-032

<150> EP18201637.8
<151> 0025-04-09

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 498
<212> PRT
<213> Artificial Sequence


<220> 
<223> Msp1 protein

<400> 1
Met Val Asp Ser Lys Lys Val Leu Ser Val Thr Ala Gly Phe Val Gly 
1               5                   10                  15      
Ala Ala Gly Leu Ala Ala Leu Ala Thr Gly Ala Asn Thr Val Ser Ala 
            20                  25                  30          
Ser Thr Gly Thr Val Ser Tyr Lys Ser Gly Ala Thr Thr Val Trp Asn 
        35                  40                  45              
Ser Pro Ser Trp His Gln Val Lys Arg Tyr Val Thr Phe Gly Asp Thr 
    50                  55                  60                  
Val Gln Leu Leu Gly Lys Thr Val Asp Gln Asn Gly Ala Thr Trp Tyr 
65                  70                  75                  80  
Lys Val Gly Asp Asn Gln Trp Ile Pro Glu Leu Tyr Leu Asn Val Ala 
                85                  90                  95      
Gly Lys Thr Ala Thr Val Glu Thr Pro Ser Ser Ala Ala Ser Gln Thr 
            100                 105                 110         
Ala Val Ser Gln Ala Pro Ala Ser Gln Ala Pro Thr Ser Gln Ala Pro 
        115                 120                 125             
Ala Thr Gln Thr Pro Ala Ala Pro Gln Thr Asp Thr Gln Thr Ala Asn 
    130                 135                 140                 
Thr Gln Leu Tyr Val Lys Asn Ile Gly Ser Ala Val Thr Val Trp Thr 
145                 150                 155                 160 
Thr Pro Ala Tyr Thr His Ala Thr Gly Gln Tyr Leu Glu Gly Ser Gln 
                165                 170                 175     
Thr Leu Thr Ala Val Ala Gln Gln Gln Ala Asn Gly Glu Thr Trp Tyr 
            180                 185                 190         
Arg Leu Ala Asn Gly Gly Tyr Val Pro Ala Arg Phe Val Ser Thr Thr 
        195                 200                 205             
Pro Val Ala Val Thr Pro Gln Pro Ala Ala Pro Gln Ser Asn Glu Ala 
    210                 215                 220                 
Ser Val Ala Ser Thr Asn Thr Asn Ala Ala Asn Asp Ser Ala Ala Ala 
225                 230                 235                 240 
Ser Ser Ala Ala Ala Ser Gln Ala Ala Ala Ser Ser Ala Ala Ala Ser 
                245                 250                 255     
Thr Ala Ala Ala Asn Ala Ala Val Ala Ser Ala Asn Ala Thr Ala Ser 
            260                 265                 270         
Gln Ala Ala Ala Ser Glu Ala Ala Ala Ser Gln Ala Ala Ala Ser Gln 
        275                 280                 285             
Ala Ala Ala Ser Gln Ala Ala Ala Ser Gln Ala Ala Ala Ser Gln Ala 
    290                 295                 300                 
Ala Ala Ser Gln Ala Ala Ala Ser Gln Ala Ala Ala Ser Gln Ala Ala 
305                 310                 315                 320 
Ala Ser Gln Ala Ala Ala Ser Gln Ala Ala Ala Asn Ala Ala Gln Gln 
                325                 330                 335     
Ala Pro Ala Asn Gln Ala Asn Val Thr Thr Thr Gln Val Asn Ala Asn 
            340                 345                 350         
Gln Ala Gln Gln Gln Thr Ala Thr Ala Thr Pro Ala Val Asn Thr Ser 
        355                 360                 365             
Asn Gln Thr Ala Ala Val Ser Ala Ser Arg Gln Ala Lys Ile Gln Ala 
    370                 375                 380                 
Val Ile Ala Ile Ala Glu Gln Gln Val Gly Lys Pro Tyr Val Trp Gly 
385                 390                 395                 400 
Gly Lys Gly Pro Asn Ser Phe Asp Cys Ser Gly Leu Met Tyr Tyr Ala 
                405                 410                 415     
Phe Leu Asn Gly Ala Gly Val Asn Ile Gly Gly Trp Thr Val Pro Gln 
            420                 425                 430         
Glu Ser Ser Gly Thr Gln Val Ser Leu Ser Ala Leu Gln Pro Gly Asp 
        435                 440                 445             
Leu Leu Phe Trp Gly Ser His Gly Ser Thr Tyr His Val Ala Leu Tyr 
    450                 455                 460                 
Ile Gly Gly Gly Thr Met Ile Gln Ala Pro Gln Pro Gly Glu Asn Val 
465                 470                 475                 480 
Lys Tyr Thr Ala Leu Ala Tyr Phe Met Pro Asp Phe Ala Val Arg Pro 
                485                 490                 495     
Ser Leu 
        

<210> 2
<211> 1498
<212> DNA
<213> Artificial Sequence


<220> 
<223> Msp1 gene

<400> 2
atggtagatt ctaagaaagt attgtcagta acggcaggct tcgttggtgc tgccggtctg      60

gcggctttag caaccggagc caataccgtt tctgcatcga cagggacggt cagttacaaa     120

tccggtgcga ccaccgtatg gaatagtcca tcatggcacc aagtcaaacg ctacgtgact     180

tttggggaca cggtgcagct attgggtaaa accgttgacc aaaatggtgc tacttggtat     240

aaagttggcg acaatcagtg gattccggaa ttgtatttga atgttgcggg taaaactgcc     300

acggttgaaa caccgagttc ggcagcaagt caaactgctg tcagccaagc accggctagt     360

caggcgccta caagccaagc accagcaacc caaacacctg cdagcaccac aaaccgatac     420

ccaaacagct aatacacagc tttatgtcaa gaatatcggt tcagcagtta ccgtatggac     480

aaccccggcg tatactcatg cgacaggtca atatctagaa ggcagccaga cactgacggc     540

tgttgcccag cagcaagcaa atggtgaaac atggtatcgg cttgccaacg gcggttatgt     600

tcctgcacgg tttgttagca caacaccagt ggctgtaaca ccacaacctg cagcgccaca     660

gtcaaatgag gccagtgttg cgtcaacgaa caccaatgca gctaatgatt ctgccgctgc     720

ttctagtgca gccgcatcgc aagccgcagc ttccagtgct gccgcttcta cagcagcagc     780

taatgcagca gtagcatctg caaatgccac tgcatcacaa gcagcggctt ctgaagctgc     840

tgcatctcag gcggctgcat cacaagccgc agcgtctcag gctgccgcat cgcaagccgc     900

agcatcacaa gctgcagcgt ctcaggcggc tgcatcacaa gccgccgctt cacaagctgc     960

agcgtcccag gctgccgcat cacaagcagc tgcaaatgct gcccagcagg cgccggctaa    1020

ccaggcaaat gttacaacaa cacaggttaa tgccaatcaa gctcaacagc aaaccgctac    1080

ggctacacca gcagttaaca cgtctaatca gacggcggcc gtaagtgctt caagacaggc    1140

caagattcaa gcagtgattg ccattgccga acaacaagtt ggtaagccat acgtttgggg    1200

cggtaagggg cctaacagct ttgattgctc cggtttgatg tattacgctt tcctgaatgg    1260

cgctggcgtc aacattggtg gctggacagt tcctcaagaa tcttcaggga cacaagtttc    1320

actgagtgct ctgcaacccg gtgacttact cttctggggt agtcacggtt caacctatca    1380

cgttgccctg tacatcggtg gcggcacgat gattcaggcg ccacagccag gtgaaaatgt    1440

taaatatacc gcgctggcat acttcatgcc tgactttgcg gttcgcccgt cactataa      1498


<210> 3
<211> 217
<212> PRT
<213> Artificial Sequence


<220> 
<223> Msp1 protein - chitinase domain

<400> 3
Met Val Asp Ser Lys Lys Val Leu Ser Val Thr Ala Gly Phe Val Gly 
1               5                   10                  15      
Ala Ala Gly Leu Ala Ala Leu Ala Thr Gly Ala Asn Thr Val Ser Ala 
            20                  25                  30          
Ser Thr Gly Thr Val Ser Tyr Lys Ser Gly Ala Thr Thr Val Trp Asn 
        35                  40                  45              
Ser Pro Ser Trp His Gln Val Lys Arg Tyr Val Thr Phe Gly Asp Thr 
    50                  55                  60                  
Val Gln Leu Leu Gly Lys Thr Val Asp Gln Asn Gly Ala Thr Trp Tyr 
65                  70                  75                  80  
Lys Val Gly Asp Asn Gln Trp Ile Pro Glu Leu Tyr Leu Asn Val Ala 
                85                  90                  95      
Gly Lys Thr Ala Thr Val Glu Thr Pro Ser Ser Ala Ala Ser Gln Thr 
            100                 105                 110         
Ala Val Ser Gln Ala Pro Ala Ser Gln Ala Pro Thr Ser Gln Ala Pro 
        115                 120                 125             
Ala Thr Gln Thr Pro Ala Ala Pro Gln Thr Asp Thr Gln Thr Ala Asn 
    130                 135                 140                 
Thr Gln Leu Tyr Val Lys Asn Ile Gly Ser Ala Val Thr Val Trp Thr 
145                 150                 155                 160 
Thr Pro Ala Tyr Thr His Ala Thr Gly Gln Tyr Leu Glu Gly Ser Gln 
                165                 170                 175     
Thr Leu Thr Ala Val Ala Gln Gln Gln Ala Asn Gly Glu Thr Trp Tyr 
            180                 185                 190         
Arg Leu Ala Asn Gly Gly Tyr Val Pro Ala Arg Phe Val Ser Thr Thr 
        195                 200                 205             
Pro Val Ala Val Thr Pro Gln Pro Ala 
    210                 215         

<210> 4
<211> 651
<212> PRT
<213> Artificial Sequence


<220> 
<223> Msp1 gene - chitinase domain

<400> 4
Ala Thr Gly Gly Thr Ala Gly Ala Thr Thr Cys Thr Ala Ala Gly Ala 
1               5                   10                  15      
Ala Ala Gly Thr Ala Thr Thr Gly Thr Cys Ala Gly Thr Ala Ala Cys 
            20                  25                  30          
Gly Gly Cys Ala Gly Gly Cys Thr Thr Cys Gly Thr Thr Gly Gly Thr 
        35                  40                  45              
Gly Cys Thr Gly Cys Cys Gly Gly Thr Cys Thr Gly Gly Cys Gly Gly 
    50                  55                  60                  
Cys Thr Thr Thr Ala Gly Cys Ala Ala Cys Cys Gly Gly Ala Gly Cys 
65                  70                  75                  80  
Cys Ala Ala Thr Ala Cys Cys Gly Thr Thr Thr Cys Thr Gly Cys Ala 
                85                  90                  95      
Thr Cys Gly Ala Cys Ala Gly Gly Gly Ala Cys Gly Gly Thr Cys Ala 
            100                 105                 110         
Gly Thr Thr Ala Cys Ala Ala Ala Thr Cys Cys Gly Gly Thr Gly Cys 
        115                 120                 125             
Gly Ala Cys Cys Ala Cys Cys Gly Thr Ala Thr Gly Gly Ala Ala Thr 
    130                 135                 140                 
Ala Gly Thr Cys Cys Ala Thr Cys Ala Thr Gly Gly Cys Ala Cys Cys 
145                 150                 155                 160 
Ala Ala Gly Thr Cys Ala Ala Ala Cys Gly Cys Thr Ala Cys Gly Thr 
                165                 170                 175     
Gly Ala Cys Thr Thr Thr Thr Gly Gly Gly Gly Ala Cys Ala Cys Gly 
            180                 185                 190         
Gly Thr Gly Cys Ala Gly Cys Thr Ala Thr Thr Gly Gly Gly Thr Ala 
        195                 200                 205             
Ala Ala Ala Cys Cys Gly Thr Thr Gly Ala Cys Cys Ala Ala Ala Ala 
    210                 215                 220                 
Thr Gly Gly Thr Gly Cys Thr Ala Cys Thr Thr Gly Gly Thr Ala Thr 
225                 230                 235                 240 
Ala Ala Ala Gly Thr Thr Gly Gly Cys Gly Ala Cys Ala Ala Thr Cys 
                245                 250                 255     
Ala Gly Thr Gly Gly Ala Thr Thr Cys Cys Gly Gly Ala Ala Thr Thr 
            260                 265                 270         
Gly Thr Ala Thr Thr Thr Gly Ala Ala Thr Gly Thr Thr Gly Cys Gly 
        275                 280                 285             
Gly Gly Thr Ala Ala Ala Ala Cys Thr Gly Cys Cys Ala Cys Gly Gly 
    290                 295                 300                 
Thr Thr Gly Ala Ala Ala Cys Ala Cys Cys Gly Ala Gly Thr Thr Cys 
305                 310                 315                 320 
Gly Gly Cys Ala Gly Cys Ala Ala Gly Thr Cys Ala Ala Ala Cys Thr 
                325                 330                 335     
Gly Cys Thr Gly Thr Cys Ala Gly Cys Cys Ala Ala Gly Cys Ala Cys 
            340                 345                 350         
Cys Gly Gly Cys Thr Ala Gly Thr Cys Ala Gly Gly Cys Gly Cys Cys 
        355                 360                 365             
Thr Ala Cys Ala Ala Gly Cys Cys Ala Ala Gly Cys Ala Cys Cys Ala 
    370                 375                 380                 
Gly Cys Ala Ala Cys Cys Cys Ala Ala Ala Cys Ala Cys Cys Thr Gly 
385                 390                 395                 400 
Cys Ala Gly Cys Ala Cys Cys Ala Cys Ala Ala Ala Cys Cys Gly Ala 
                405                 410                 415     
Thr Ala Cys Cys Cys Ala Ala Ala Cys Ala Gly Cys Thr Ala Ala Thr 
            420                 425                 430         
Ala Cys Ala Cys Ala Gly Cys Thr Thr Thr Ala Thr Gly Thr Cys Ala 
        435                 440                 445             
Ala Gly Ala Ala Thr Ala Thr Cys Gly Gly Thr Thr Cys Ala Gly Cys 
    450                 455                 460                 
Ala Gly Thr Thr Ala Cys Cys Gly Thr Ala Thr Gly Gly Ala Cys Ala 
465                 470                 475                 480 
Ala Cys Cys Cys Cys Gly Gly Cys Gly Thr Ala Thr Ala Cys Thr Cys 
                485                 490                 495     
Ala Thr Gly Cys Gly Ala Cys Ala Gly Gly Thr Cys Ala Ala Thr Ala 
            500                 505                 510         
Thr Cys Thr Ala Gly Ala Ala Gly Gly Cys Ala Gly Cys Cys Ala Gly 
        515                 520                 525             
Ala Cys Ala Cys Thr Gly Ala Cys Gly Gly Cys Thr Gly Thr Thr Gly 
    530                 535                 540                 
Cys Cys Cys Ala Gly Cys Ala Gly Cys Ala Ala Gly Cys Ala Ala Ala 
545                 550                 555                 560 
Thr Gly Gly Thr Gly Ala Ala Ala Cys Ala Thr Gly Gly Thr Ala Thr 
                565                 570                 575     
Cys Gly Gly Cys Thr Thr Gly Cys Cys Ala Ala Cys Gly Gly Cys Gly 
            580                 585                 590         
Gly Thr Thr Ala Thr Gly Thr Thr Cys Cys Thr Gly Cys Ala Cys Gly 
        595                 600                 605             
Gly Thr Thr Thr Gly Thr Thr Ala Gly Cys Ala Cys Ala Ala Cys Ala 
    610                 615                 620                 
Cys Cys Ala Gly Thr Gly Gly Cys Thr Gly Thr Ala Ala Cys Ala Cys 
625                 630                 635                 640 
Cys Ala Cys Ala Ala Cys Cys Thr Gly Cys Ala 
                645                 650     

