                         SEQUENCE LISTING

<110>  Conagen Inc.
 
<120>  Biosynthesis of Homoeriodictyol

<130>  CCN-1901WO00

<150>  62744962
<151>  2018-10-12

<150>  62914560
<151>  2019-10-14

<160>  70    

<170>  PatentIn version 3.5

<210>  1
<211>  364
<212>  PRT
<213>  Populus balsamifera subsp. trichocarpa

<400>  1

Met Gly Ser Thr Gly Glu Thr Gln Met Thr Pro Thr Gln Val Ser Asp 
1               5                   10                  15      


Glu Glu Ala His Leu Phe Ala Met Gln Leu Ala Ser Ala Ser Val Leu 
            20                  25                  30          


Pro Met Ile Leu Lys Thr Ala Ile Glu Leu Asp Leu Leu Glu Ile Met 
        35                  40                  45              


Ala Lys Ala Gly Pro Gly Ala Phe Leu Ser Thr Ser Glu Ile Ala Ser 
    50                  55                  60                  


His Leu Pro Thr Lys Asn Pro Asp Ala Pro Val Met Leu Asp Arg Ile 
65                  70                  75                  80  


Leu Arg Leu Leu Ala Ser Tyr Ser Ile Leu Thr Cys Ser Leu Lys Asp 
                85                  90                  95      


Leu Pro Asp Gly Lys Val Glu Arg Leu Tyr Gly Leu Ala Pro Val Cys 
            100                 105                 110         


Lys Phe Leu Thr Lys Asn Glu Asp Gly Val Ser Val Ser Pro Leu Cys 
        115                 120                 125             


Leu Met Asn Gln Asp Lys Val Leu Met Glu Ser Trp Tyr Tyr Leu Lys 
    130                 135                 140                 


Asp Ala Ile Leu Asp Gly Gly Ile Pro Phe Asn Lys Ala Tyr Gly Met 
145                 150                 155                 160 


Thr Ala Phe Glu Tyr His Gly Thr Asp Pro Arg Phe Asn Lys Val Phe 
                165                 170                 175     


Asn Lys Gly Met Ser Asp His Ser Thr Ile Thr Met Lys Lys Leu Leu 
            180                 185                 190         


Glu Thr Tyr Lys Gly Phe Glu Gly Leu Thr Ser Leu Val Asp Val Gly 
        195                 200                 205             


Gly Gly Thr Gly Ala Val Val Asn Thr Ile Val Ser Lys Tyr Pro Ser 
    210                 215                 220                 


Ile Lys Gly Ile Asn Phe Asp Leu Pro His Val Ile Glu Asp Ala Pro 
225                 230                 235                 240 


Ser Tyr Pro Gly Val Glu His Val Gly Gly Asp Met Phe Val Ser Val 
                245                 250                 255     


Pro Lys Ala Asp Ala Val Phe Met Lys Trp Ile Cys His Asp Trp Ser 
            260                 265                 270         


Asp Ala His Cys Leu Lys Phe Leu Lys Asn Cys Tyr Asp Ala Leu Pro 
        275                 280                 285             


Glu Asn Gly Lys Val Ile Leu Val Glu Cys Ile Leu Pro Val Ala Pro 
    290                 295                 300                 


Asp Thr Ser Leu Ala Thr Lys Gly Val Val His Ile Asp Val Ile Met 
305                 310                 315                 320 


Leu Ala His Asn Pro Gly Gly Lys Glu Arg Thr Glu Lys Glu Phe Glu 
                325                 330                 335     


Gly Leu Ala Lys Gly Ala Gly Phe Gln Gly Phe Glu Val Met Cys Cys 
            340                 345                 350         


Ala Phe Asn Thr His Val Ile Glu Phe Arg Lys Asn 
        355                 360                 


<210>  2
<211>  1095
<212>  DNA
<213>  Populus balsamifera subsp. trichocarpa

<400>  2
atgggttcga caggtgaaac tcagatgact ccaactcagg tatcagatga agaggcacac       60

ctctttgcca tgcaactagc cagtgcttca gttctaccaa tgatcctcaa aacagccatt      120

gaactcgacc ttcttgaaat catggctaaa gctggccctg gtgctttctt gtccacatct      180

gagatagctt ctcacctccc taccaaaaac cctgatgcgc ctgtcatgtt agaccgtatc      240

ttgcgcctcc tggctagcta ctccattctt acttgctctc tgaaagatct tcctgatggg      300

aaagttgaga gactgtatgg ccttgctcct gtttgcaaat tcttgaccaa gaacgaggac      360

ggtgtctctg tcagccctct ctgtctcatg aaccaggaca aggtcctcat ggaaagctgg      420

tattatttga aagatgcaat tcttgatgga ggaattccat ttaacaaggc ctatgggatg      480

actgcatttg aatatcatgg cacggatcca agattcaaca aggtgttcaa taagggaatg      540

tctgaccact ctaccattac catgaagaag cttcttgaga cctacaaagg ctttgaaggc      600

ctcacatcct tggtggatgt tggtggtggg actggagctg tcgttaacac catcgtctct      660

aaataccctt caattaaggg cattaacttt gatctgcccc acgtcattga ggatgcccca      720

tcttatcccg gtgtggagca tgttggtggg gacatgtttg ttagcgtgcc caaagcagat      780

gccgttttca tgaagtggat atgccatgat tggagcgacg cacactgctt aaaattcttg      840

aagaattgct atgacgcctt gccggaaaac ggcaaggtga tacttgttga gtgcattctt      900

cccgtggctc ctgacacaag ccttgccacc aagggagtcg tgcacattga tgttatcatg      960

ctggcgcaca accccggtgg gaaagagagg accgaaaagg aatttgaggg cttagcaaag     1020

ggagctggct ttcaaggttt tgaagtaatg tgctgtgcat tcaacacaca tgtcattgaa     1080

ttccgcaaga actaa                                                      1095


<210>  3
<211>  368
<212>  PRT
<213>  Oryza sativa

<400>  3

Met Gly Ser Thr Ala Ala Asp Met Ala Ala Ala Ala Asp Glu Glu Ala 
1               5                   10                  15      


Cys Met Tyr Ala Leu Gln Leu Ala Ser Ser Ser Ile Leu Pro Met Thr 
            20                  25                  30          


Leu Lys Asn Ala Ile Glu Leu Gly Leu Leu Glu Thr Leu Gln Ser Ala 
        35                  40                  45              


Ala Val Ala Gly Gly Gly Gly Lys Ala Ala Leu Leu Thr Pro Ala Glu 
    50                  55                  60                  


Val Ala Asp Lys Leu Pro Ser Lys Ala Asn Pro Ala Ala Ala Asp Met 
65                  70                  75                  80  


Val Asp Arg Met Leu Arg Leu Leu Ala Ser Tyr Asn Val Val Arg Cys 
                85                  90                  95      


Glu Met Glu Glu Gly Ala Asp Gly Lys Leu Ser Arg Arg Tyr Ala Ala 
            100                 105                 110         


Ala Pro Val Cys Lys Trp Leu Thr Pro Asn Glu Asp Gly Val Ser Met 
        115                 120                 125             


Ala Ala Leu Ala Leu Met Asn Gln Asp Lys Val Leu Met Glu Ser Trp 
    130                 135                 140                 


Tyr Tyr Leu Lys Asp Ala Val Leu Asp Gly Gly Ile Pro Phe Asn Lys 
145                 150                 155                 160 


Ala Tyr Gly Met Thr Ala Phe Glu Tyr His Gly Thr Asp Ala Arg Phe 
                165                 170                 175     


Asn Arg Val Phe Asn Glu Gly Met Lys Asn His Ser Val Ile Ile Thr 
            180                 185                 190         


Lys Lys Leu Leu Asp Leu Tyr Thr Gly Phe Asp Ala Ala Ser Thr Val 
        195                 200                 205             


Val Asp Val Gly Gly Gly Val Gly Ala Thr Val Ala Ala Val Val Ser 
    210                 215                 220                 


Arg His Pro His Ile Arg Gly Ile Asn Tyr Asp Leu Pro His Val Ile 
225                 230                 235                 240 


Ser Glu Ala Pro Pro Phe Pro Gly Val Glu His Val Gly Gly Asp Met 
                245                 250                 255     


Phe Ala Ser Val Pro Arg Gly Gly Asp Ala Ile Leu Met Lys Trp Ile 
            260                 265                 270         


Leu His Asp Trp Ser Asp Glu His Cys Ala Arg Leu Leu Lys Asn Cys 
        275                 280                 285             


Tyr Asp Ala Leu Pro Glu His Gly Lys Val Val Val Val Glu Cys Val 
    290                 295                 300                 


Leu Pro Glu Ser Ser Asp Ala Thr Ala Arg Glu Gln Gly Val Phe His 
305                 310                 315                 320 


Val Asp Met Ile Met Leu Ala His Asn Pro Gly Gly Lys Glu Arg Tyr 
                325                 330                 335     


Glu Arg Glu Phe Arg Glu Leu Ala Arg Ala Ala Gly Phe Thr Gly Phe 
            340                 345                 350         


Lys Ala Thr Tyr Ile Tyr Ala Asn Ala Trp Ala Ile Glu Phe Thr Lys 
        355                 360                 365             


<210>  4
<211>  1107
<212>  DNA
<213>  Oryza sativa

<400>  4
atgggtagca ccgcagcaga tatggcagca gcagccgatg aagaggcatg tatgtatgca       60

ctgcagctgg caagcagcag tattctgccg atgaccctga aaaatgcaat tgaactgggt      120

ctgctggaaa ccctgcagag cgcagcagtt gccggtggtg gtggtaaagc agcactgctg      180

acaccggcag aagttgcaga taaactgccg agcaaagcaa atccggcagc agcggatatg      240

gttgatcgta tgctgcgtct gctggcgagc tataatgttg ttcgttgtga aatggaagag      300

ggtgcagatg gtaaactgag ccgtcgttat gcagcagcac cggtttgtaa atggctgacc      360

ccgaatgaag atggtgttag catggcagca ctggcactga tgaatcagga taaagttctg      420

atggaaagct ggtactatct gaaagatgca gttctggatg gtggtatccc gtttaacaaa      480

gcctatggta tgaccgcctt tgaatatcat ggcaccgatg cacgttttaa tcgcgttttt      540

aatgagggca tgaaaaacca tagcgtgatc atcaccaaaa aactgctgga tctgtatacc      600

ggttttgatg ccgcaagcac cgttgttgat gttggtggtg gcgttggtgc aaccgttgca      660

gccgttgtta gccgtcatcc gcatattcgt ggtattaact atgatctgcc gcatgttatt      720

agcgaagcac cgccttttcc gggtgttgaa catgtgggtg gtgatatgtt tgcaagcgtt      780

ccgcgtggtg gtgatgcaat tctgatgaaa tggattctgc atgattggtc cgatgaacat      840

tgtgcacgtc tgctgaaaaa ttgttatgat gccctgccgg aacatggtaa agttgttgtg      900

gttgaatgtg ttctgccgga aagcagtgat gcaaccgcac gtgaacaggg tgtgtttcat      960

gttgatatga ttatgctggc acataatccg ggtggcaaag aacgttatga acgtgaattt     1020

cgtgaactgg cacgtgcagc aggttttacg ggttttaaag caacctatat ctatgcaaat     1080

gcctgggcca ttgagttcac caaataa                                         1107


<210>  5
<211>  363
<212>  PRT
<213>  Arabidopsis thaliana

<400>  5

Met Gly Ser Thr Ala Glu Thr Gln Leu Thr Pro Val Gln Val Thr Asp 
1               5                   10                  15      


Asp Glu Ala Ala Leu Phe Ala Met Gln Leu Ala Ser Ala Ser Val Leu 
            20                  25                  30          


Pro Met Ala Leu Lys Ser Ala Leu Glu Leu Asp Leu Leu Glu Ile Met 
        35                  40                  45              


Ala Lys Asn Gly Ser Pro Met Ser Pro Thr Glu Ile Ala Ser Lys Leu 
    50                  55                  60                  


Pro Thr Lys Asn Pro Glu Ala Pro Val Met Leu Asp Arg Ile Leu Arg 
65                  70                  75                  80  


Leu Leu Thr Ser Tyr Ser Val Leu Thr Cys Ser Asn Arg Lys Leu Ser 
                85                  90                  95      


Gly Asp Gly Val Glu Arg Ile Tyr Gly Leu Gly Pro Val Cys Lys Tyr 
            100                 105                 110         


Leu Thr Lys Asn Glu Asp Gly Val Ser Ile Ala Ala Leu Cys Leu Met 
        115                 120                 125             


Asn Gln Asp Lys Val Leu Met Glu Ser Trp Tyr His Leu Lys Asp Ala 
    130                 135                 140                 


Ile Leu Asp Gly Gly Ile Pro Phe Asn Lys Ala Tyr Gly Met Ser Ala 
145                 150                 155                 160 


Phe Glu Tyr His Gly Thr Asp Pro Arg Phe Asn Lys Val Phe Asn Asn 
                165                 170                 175     


Gly Met Ser Asn His Ser Thr Ile Thr Met Lys Lys Ile Leu Glu Thr 
            180                 185                 190         


Tyr Lys Gly Phe Glu Gly Leu Thr Ser Leu Val Asp Val Gly Gly Gly 
        195                 200                 205             


Ile Gly Ala Thr Leu Lys Met Ile Val Ser Lys Tyr Pro Asn Leu Lys 
    210                 215                 220                 


Gly Ile Asn Phe Asp Leu Pro His Val Ile Glu Asp Ala Pro Ser His 
225                 230                 235                 240 


Pro Gly Ile Glu His Val Gly Gly Asp Met Phe Val Ser Val Pro Lys 
                245                 250                 255     


Gly Asp Ala Ile Phe Met Lys Trp Ile Cys His Asp Trp Ser Asp Glu 
            260                 265                 270         


His Cys Val Lys Phe Leu Lys Asn Cys Tyr Glu Ser Leu Pro Glu Asp 
        275                 280                 285             


Gly Lys Val Ile Leu Ala Glu Cys Ile Leu Pro Glu Thr Pro Asp Ser 
    290                 295                 300                 


Ser Leu Ser Thr Lys Gln Val Val His Val Asp Cys Ile Met Leu Ala 
305                 310                 315                 320 


His Asn Pro Gly Gly Lys Glu Arg Thr Glu Lys Glu Phe Glu Ala Leu 
                325                 330                 335     


Ala Lys Ala Ser Gly Phe Lys Gly Ile Lys Val Val Cys Asp Ala Phe 
            340                 345                 350         


Gly Val Asn Leu Ile Glu Leu Leu Lys Lys Leu 
        355                 360             


<210>  6
<211>  1092
<212>  DNA
<213>  Arabidopsis thaliana

<400>  6
atgggttcaa cggcagagac acaattaact ccggtgcaag tcaccgacga cgaagctgcc       60

ctcttcgcca tgcaactagc cagtgcttcc gttcttccga tggctttaaa atccgcctta      120

gagcttgacc ttcttgagat tatggccaag aatggttctc ccatgtctcc taccgagatc      180

gcttctaaac ttccgaccaa aaatcctgaa gctccggtca tgctcgaccg tatcctccgt      240

cttcttacgt cttactccgt cttaacctgc tccaaccgta aactttccgg tgatggcgtt      300

gaacggattt acgggcttgg tccggtttgc aagtatttga ccaagaacga agatggtgtt      360

tccattgctg ctctttgtct tatgaaccaa gacaaggttc tcatggaaag ctggtaccat      420

ttgaaggatg caattcttga tggtgggatt ccattcaaca aggcttatgg aatgagcgcg      480

ttcgagtacc acgggactga ccctagattc aacaaggtct ttaacaatgg aatgtctaac      540

cattccacaa tcaccatgaa gaagattctt gagacctata agggttttga aggattgact      600

tctttggttg atgttggtgg tggcattggt gctacactca aaatgattgt ctccaagtac      660

cctaatctta aaggcatcaa ctttgatctc ccacatgtca tcgaagatgc tccttctcat      720

cctggtattg agcatgttgg aggagatatg tttgtaagtg tccctaaagg tgatgccata      780

ttcatgaagt ggatatgtca tgactggagt gacgaacatt gcgtgaaatt cttgaagaac      840

tgctacgagt cacttccaga ggatggaaaa gtgatattag cagagtgtat acttccagag      900

acaccagact caagcctctc aaccaaacaa gtagtccatg tcgattgcat tatgttggct      960

cacaatcccg gaggcaaaga acgaaccgag aaagagtttg aggcattagc caaagcatca     1020

ggcttcaagg gcatcaaagt tgtctgcgac gcttttggtg ttaaccttat tgagttactc     1080

aagaagctct aa                                                         1092


<210>  7
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1 Forward Primer

<400>  7
ggaattccat atgggttcga caggtgaaac tcagatg                                37


<210>  8
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1 reverse primer

<400>  8
aaggaaaaaa gcggccgctt agttcttgcg gaattcaatg acatg                       45


<210>  9
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AtOMT1 forward primer

<400>  9
ggaattccat atgggttcaa cggcagagac acaattaac                              39


<210>  10
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AtOM1 reverse primer

<400>  10
aaggaaaaaa gcggccgctt agagcttctt gagtaactca ataagg                      46


<210>  11
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MT1-I316G forward primer

<400>  11
gtcgtgcacg gcgatgttat catgctggcg cacaacccc                              39


<210>  12
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316G

<400>  12
taacatcgcc gtgcacgact cccttggtgg caaggcttg                              39


<210>  13
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316L forward primer

<400>  13
gtcgtgcacc tggatgttat catgctggcg cacaacccc                              39


<210>  14
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316L reverse primer

<400>  14
taacatccag gtgcacgact cccttggtgg caaggcttg                              39


<210>  15
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316P forward primer

<400>  15
gtcgtgcacc cggatgttat catgctggcg cacaacccc                              39


<210>  16
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316P reverse primer

<400>  16
taacatccgg gtgcacgact cccttggtgg caaggcttg                              39


<210>  17
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316F forward primer

<400>  17
gtcgtgcact ttgatgttat catgctggcg cacaacccc                              39


<210>  18
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316F reverse primer

<400>  18
taacatcaaa gtgcacgact cccttggtgg caaggcttg                              39


<210>  19
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316W forward primer

<400>  19
gtcgtgcact gggatgttat catgctggcg cacaacccc                              39


<210>  20
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316W reverse primer

<400>  20
taacatccca gtgcacgact cccttggtgg caaggcttg                              39


<210>  21
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316Y forward primer

<400>  21
gtcgtgcact atgatgttat catgctggcg cacaacccc                              39


<210>  22
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316Y reverse primer

<400>  22
taacatcata gtgcacgact cccttggtgg caaggcttg                              39


<210>  23
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316D forward primer

<400>  23
gtcgtgcacg atgatgttat catgctggcg cacaacccc                              39


<210>  24
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316D reverse primer

<400>  24
taacatcatc gtgcacgact cccttggtgg caaggcttg                              39


<210>  25
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316E forward primer

<400>  25
gtcgtgcacg aagatgttat catgctggcg cacaacccc                              39


<210>  26
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316E reverse primer

<400>  26
taacatcttc gtgcacgact cccttggtgg caaggcttg                              39


<210>  27
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316R forward primer

<400>  27
gtcgtgcacc gcgatgttat catgctggcg cacaacccc                              39


<210>  28
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316R reverse primer

<400>  28
taacatcgcg gtgcacgact cccttggtgg caaggcttg                              39


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316H forward primer

<400>  29
gtcgtgcacc atgatgttat catgctggcg cacaacccc                              39


<210>  30
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316H reverse primer

<400>  30
taacatcatg gtgcacgact cccttggtgg caaggcttg                              39


<210>  31
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316K forward primer

<400>  31
gtcgtgcaca aagatgttat catgctggcg cacaacccc                              39


<210>  32
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316K reverse primer

<400>  32
taacatcttt gtgcacgact cccttggtgg caaggcttg                              39


<210>  33
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316S forward primer

<400>  33
gtcgtgcaca gcgatgttat catgctggcg cacaacccc                              39


<210>  34
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316S reverse primer

<400>  34
taacatcgct gtgcacgact cccttggtgg caaggcttg                              39


<210>  35
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316T forward primer

<400>  35
gtcgtgcaca ccgatgttat catgctggcg cacaacccc                              39


<210>  36
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316T reverse primer

<400>  36
taacatcggt gtgcacgact cccttggtgg caaggcttg                              39


<210>  37
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316C forward primer

<400>  37
gtcgtgcact gcgatgttat catgctggcg cacaacccc                              39


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316C reverse primer

<400>  38
taacatcgca gtgcacgact cccttggtgg caaggcttg                              39


<210>  39
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316M forward primer

<400>  39
gtcgtgcaca tggatgttat catgctggcg cacaacccc                              39


<210>  40
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316M reverse primer

<400>  40
taacatccat gtgcacgact cccttggtgg caaggcttg                              39


<210>  41
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316N forward primer

<400>  41
gtcgtgcaca acgatgttat catgctggcg cacaacccc                              39


<210>  42
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316N reverse primer

<400>  42
taacatcgtt gtgcacgact cccttggtgg caaggcttg                              39


<210>  43
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316Q forward primer

<400>  43
gtcgtgcacc aggatgttat catgctggcg cacaacccc                              39


<210>  44
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PtCOMT1-I316Q reverse primer

<400>  44
taacatcctg gtgcacgact cccttggtgg caaggcttg                              39


<210>  45
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OsOMT9-V321A forward primer

<400>  45
gtgtttcatg cggatatgat tatgctggca cataatccgg                             40


<210>  46
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OsOMT9-V321A reverse primer

<400>  46
aatcatatcc gcatgaaaca caccctgttc acgtgcgg                               38


<210>  47
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AtOMT1-V314A forward primer

<400>  47
aatcatatcc gcatgaaaca caccctgttc acgtgcgg                               38


<210>  48
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AtOMT1-V3141A reverse primer

<400>  48
taatgcaatc ggcatggact acttgtttgg ttgagaggc                              39


<210>  49
<211>  2325
<212>  DNA
<213>  Escherichia coli

<400>  49
ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc atcgtttagg       60

caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat      120

aacaatttca actataagaa ggagatatac atatggcgga tccgaattcg gcgcgccaga      180

tctcaattgg atatcggccg gccgacgtcg gtaccgcggc cgccaccgct gagcaataac      240

tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa      300

ctatatccgg gtaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg      360

taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc ttcggtagaa      420

aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa      480

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc      540

gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag      600

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg      660

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga      720

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc      780

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc      840

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga      900

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt      960

cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg     1020

aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt acatttgggc     1080

cctcatcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca     1140

tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg     1200

agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt     1260

ttttcctgtt tggtcattta ccaatgctta atcagtgagg cacctatctc agcgatctgt     1320

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     1380

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     1440

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     1500

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     1560

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     1620

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     1680

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     1740

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     1800

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     1860

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     1920

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     1980

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     2040

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     2100

aagggaataa gggcgacacg gaaatgttga atactcatag ctcctgaaaa tctcgataac     2160

tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg     2220

tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat ggaggtcagg     2280

tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtc                     2325


<210>  50
<211>  1512
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SsPvcC-M196Y mutant

<400>  50
atgacgggcg ccgaatatct ggattcgctg cgtgatggcc gtgccgtcta tattcacggc       60

gaacgcgtcc gcgatgtcac cgcgcatccg gccttccgta acagcgcgcg tagtctggcg      120

cagctgtatg atgtgctgca tgaaccggat tcgcgtggcg ttctgagcgt cccgaccgat      180

accggtaatg gcggttttac gcacccgttt ttcaaaaccg cgcgtagcgc cggtgatctg      240

gtggcagccc gcgatgcaat tgtggcctgg cagcgtcttg tttacggttg gatgggtcgt      300

acgccggatt ataaagcagc attcttcggt acgcttgaag ccaacgccga tttctatggc      360

ccgttccgtg ataacgcact ggcatggtat cgtcgtgcac aggaacgcgt gttgtatttc      420

aaccatgcga tcgtgcatcc gccggtcgat cgcgatcgcc cggccgatcg cacggcggat      480

gtgtgcgtcc acgtggaaga agaaacggat gccggccttg ttgtttcggg tgcgaaagtt      540

gtcgcgacca gcagcgccct gacgaatgcc aatctgattg cacactacgg cttaccgctg      600

cgtgataaac gctttggcgc catgttcacc gtgccgatgg atagcccggg cctgaaactg      660

ttctgtcgta ccagttatga aatgcatgcc gcggtcttag gctcgccgtt tgattacccg      720

ttaagcagcc gccttgatga aaatgattca atcatggttc ttgatcgtgt tttagtgccg      780

tgggaaaacg tgtttatgta tgatgccgcc agcgcgaacg cgtttgcgac ccgcagcggt      840

ttcctggaac gttttacctt tcatggctgt acgcgcttag cggtgaaact ggattttatt      900

gccggttgcc tgctgaaagc agttgaagtg accggcacca gcggcttccg tggtgtgcag      960

gcccagattg gcgaagtttt aaactggcgc gatatgtttt ggggtatgtc agatgcaatg     1020

gcgaaatcgc cgacggattg gcacaatggt gcggtccagc cgaatctgaa ctatggcctg     1080

gcctaccgca ccttcatggg cattggttac ccgcgtatcc gtgaaatcat tcagcagacc     1140

attggctcgg gtctgattta tttaaatagt cacgcaagcg attggaaaaa tccggaagtt     1200

cgtccgtact tagatcgcta cctgcgcggt agtcgtggtg ttgaagcgat cgatcgcgtt     1260

aaactgctta aactgctgtg ggattgcgtg ggcacggaat ttgcgggccg tcacgaactt     1320

tatgaacgca attacggtgg cgatcatgaa ggtattcgcg tgcagaccct gttgagttat     1380

caggcgcgcg gtcaggcgga tgcgcttaaa ggctttgccg atcagtgtat gtcagaatac     1440

gatctggatg gctggacccg cccggattta ttcggtccag gtgatttacc tagaccagct     1500

actggagcgt aa                                                         1512


<210>  51
<211>  503
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SsPvcC-M196Y mutant

<400>  51

Met Thr Gly Ala Glu Tyr Leu Asp Ser Leu Arg Asp Gly Arg Ala Val 
1               5                   10                  15      


Tyr Ile His Gly Glu Arg Val Arg Asp Val Thr Ala His Pro Ala Phe 
            20                  25                  30          


Arg Asn Ser Ala Arg Ser Leu Ala Gln Leu Tyr Asp Val Leu His Glu 
        35                  40                  45              


Pro Asp Ser Arg Gly Val Leu Ser Val Pro Thr Asp Thr Gly Asn Gly 
    50                  55                  60                  


Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ala Gly Asp Leu 
65                  70                  75                  80  


Val Ala Ala Arg Asp Ala Ile Val Ala Trp Gln Arg Leu Val Tyr Gly 
                85                  90                  95      


Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr Leu 
            100                 105                 110         


Glu Ala Asn Ala Asp Phe Tyr Gly Pro Phe Arg Asp Asn Ala Leu Ala 
        115                 120                 125             


Trp Tyr Arg Arg Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala Ile 
    130                 135                 140                 


Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala Asp 
145                 150                 155                 160 


Val Cys Val His Val Glu Glu Glu Thr Asp Ala Gly Leu Val Val Ser 
                165                 170                 175     


Gly Ala Lys Val Val Ala Thr Ser Ser Ala Leu Thr Asn Ala Asn Leu 
            180                 185                 190         


Ile Ala His Tyr Gly Leu Pro Leu Arg Asp Lys Arg Phe Gly Ala Met 
        195                 200                 205             


Phe Thr Val Pro Met Asp Ser Pro Gly Leu Lys Leu Phe Cys Arg Thr 
    210                 215                 220                 


Ser Tyr Glu Met His Ala Ala Val Leu Gly Ser Pro Phe Asp Tyr Pro 
225                 230                 235                 240 


Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Val Leu Asp Arg 
                245                 250                 255     


Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Ala Ser Ala 
            260                 265                 270         


Asn Ala Phe Ala Thr Arg Ser Gly Phe Leu Glu Arg Phe Thr Phe His 
        275                 280                 285             


Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys Leu 
    290                 295                 300                 


Leu Lys Ala Val Glu Val Thr Gly Thr Ser Gly Phe Arg Gly Val Gln 
305                 310                 315                 320 


Ala Gln Ile Gly Glu Val Leu Asn Trp Arg Asp Met Phe Trp Gly Met 
                325                 330                 335     


Ser Asp Ala Met Ala Lys Ser Pro Thr Asp Trp His Asn Gly Ala Val 
            340                 345                 350         


Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly Ile 
        355                 360                 365             


Gly Tyr Pro Arg Ile Arg Glu Ile Ile Gln Gln Thr Ile Gly Ser Gly 
    370                 375                 380                 


Leu Ile Tyr Leu Asn Ser His Ala Ser Asp Trp Lys Asn Pro Glu Val 
385                 390                 395                 400 


Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Val Glu Ala 
                405                 410                 415     


Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Cys Val Gly Thr 
            420                 425                 430         


Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly Asp 
        435                 440                 445             


His Glu Gly Ile Arg Val Gln Thr Leu Leu Ser Tyr Gln Ala Arg Gly 
    450                 455                 460                 


Gln Ala Asp Ala Leu Lys Gly Phe Ala Asp Gln Cys Met Ser Glu Tyr 
465                 470                 475                 480 


Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Phe Gly Pro Gly Asp Leu 
                485                 490                 495     


Pro Arg Pro Ala Thr Gly Ala 
            500             


<210>  52
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SsPvcC-M196Y-Tag

<400>  52
atgacgaccg ctagtggtac caatgcagat gtccagaatg gcgtccgccc gatgacgggc       60

gccgaatatc tggattcgct gcgtgatggc cgtgccgtct atattcacgg cgaacgcgtc      120

cgcgatgtca ccgcgcatcc ggccttccgt aacagcgcgc gtagtctggc gcagctgtat      180

gatgtgctgc atgaaccgga ttcgcgtggc gttctgagcg tcccgaccga taccggtaat      240

ggcggtttta cgcacccgtt tttcaaaacc gcgcgtagcg ccggtgatct ggtggcagcc      300

cgcgatgcaa ttgtggcctg gcagcgtctt gtttacggtt ggatgggtcg tacgccggat      360

tataaagcag cattcttcgg tacgcttgaa gccaacgccg atttctatgg cccgttccgt      420

gataacgcac tggcatggta tcgtcgtgca caggaacgcg tgttgtattt caaccatgcg      480

atcgtgcatc cgccggtcga tcgcgatcgc ccggccgatc gcacggcgga tgtgtgcgtc      540

cacgtggaag aagaaacgga tgccggcctt gttgtttcgg gtgcgaaagt tgtcgcgacc      600

agcagcgccc tgacgaatgc caatctgatt gcacactacg gcttaccgct gcgtgataaa      660

cgctttggcg ccatgttcac cgtgccgatg gatagcccgg gcctgaaact gttctgtcgt      720

accagttatg aaatgcatgc cgcggtctta ggctcgccgt ttgattaccc gttaagcagc      780

cgccttgatg aaaatgattc aatcatggtt cttgatcgtg ttttagtgcc gtgggaaaac      840

gtgtttatgt atgatgccgc cagcgcgaac gcgtttgcga cccgcagcgg tttcctggaa      900

cgttttacct ttcatggctg tacgcgctta gcggtgaaac tggattttat tgccggttgc      960

ctgctgaaag cagttgaagt gaccggcacc agcggcttcc gtggtgtgca ggcccagatt     1020

ggcgaagttt taaactggcg cgatatgttt tggggtatgt cagatgcaat ggcgaaatcg     1080

ccgacggatt ggcacaatgg tgcggtccag ccgaatctga actatggcct ggcctaccgc     1140

accttcatgg gcattggtta cccgcgtatc cgtgaaatca ttcagcagac cattggctcg     1200

ggtctgattt atttaaatag tcacgcaagc gattggaaaa atccggaagt tcgtccgtac     1260

ttagatcgct acctgcgcgg tagtcgtggt gttgaagcga tcgatcgcgt taaactgctt     1320

aaactgctgt gggattgcgt gggcacggaa tttgcgggcc gtcacgaact ttatgaacgc     1380

aattacggtg gcgatcatga aggtattcgc gtgcagaccc tgttgagtta tcaggcgcgc     1440

ggtcaggcgg atgcgcttaa aggctttgcc gatcagtgta tgtcagaata cgatctggat     1500

ggctggaccc gcccggattt attcggtcca ggtgatttac ctagaccagc tactggagcg     1560

taa                                                                   1563


<210>  53
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SsPvcC-M196Y-Tag mutant

<400>  53

Met Thr Thr Ala Ser Gly Thr Asn Ala Asp Val Gln Asn Gly Val Arg 
1               5                   10                  15      


Pro Met Thr Gly Ala Glu Tyr Leu Asp Ser Leu Arg Asp Gly Arg Ala 
            20                  25                  30          


Val Tyr Ile His Gly Glu Arg Val Arg Asp Val Thr Ala His Pro Ala 
        35                  40                  45              


Phe Arg Asn Ser Ala Arg Ser Leu Ala Gln Leu Tyr Asp Val Leu His 
    50                  55                  60                  


Glu Pro Asp Ser Arg Gly Val Leu Ser Val Pro Thr Asp Thr Gly Asn 
65                  70                  75                  80  


Gly Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ala Gly Asp 
                85                  90                  95      


Leu Val Ala Ala Arg Asp Ala Ile Val Ala Trp Gln Arg Leu Val Tyr 
            100                 105                 110         


Gly Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr 
        115                 120                 125             


Leu Glu Ala Asn Ala Asp Phe Tyr Gly Pro Phe Arg Asp Asn Ala Leu 
    130                 135                 140                 


Ala Trp Tyr Arg Arg Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala 
145                 150                 155                 160 


Ile Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Val Cys Val His Val Glu Glu Glu Thr Asp Ala Gly Leu Val Val 
            180                 185                 190         


Ser Gly Ala Lys Val Val Ala Thr Ser Ser Ala Leu Thr Asn Ala Asn 
        195                 200                 205             


Leu Ile Ala His Tyr Gly Leu Pro Leu Arg Asp Lys Arg Phe Gly Ala 
    210                 215                 220                 


Met Phe Thr Val Pro Met Asp Ser Pro Gly Leu Lys Leu Phe Cys Arg 
225                 230                 235                 240 


Thr Ser Tyr Glu Met His Ala Ala Val Leu Gly Ser Pro Phe Asp Tyr 
                245                 250                 255     


Pro Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Val Leu Asp 
            260                 265                 270         


Arg Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Ala Ser 
        275                 280                 285             


Ala Asn Ala Phe Ala Thr Arg Ser Gly Phe Leu Glu Arg Phe Thr Phe 
    290                 295                 300                 


His Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys 
305                 310                 315                 320 


Leu Leu Lys Ala Val Glu Val Thr Gly Thr Ser Gly Phe Arg Gly Val 
                325                 330                 335     


Gln Ala Gln Ile Gly Glu Val Leu Asn Trp Arg Asp Met Phe Trp Gly 
            340                 345                 350         


Met Ser Asp Ala Met Ala Lys Ser Pro Thr Asp Trp His Asn Gly Ala 
        355                 360                 365             


Val Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly 
    370                 375                 380                 


Ile Gly Tyr Pro Arg Ile Arg Glu Ile Ile Gln Gln Thr Ile Gly Ser 
385                 390                 395                 400 


Gly Leu Ile Tyr Leu Asn Ser His Ala Ser Asp Trp Lys Asn Pro Glu 
                405                 410                 415     


Val Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Val Glu 
            420                 425                 430         


Ala Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Cys Val Gly 
        435                 440                 445             


Thr Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly 
    450                 455                 460                 


Asp His Glu Gly Ile Arg Val Gln Thr Leu Leu Ser Tyr Gln Ala Arg 
465                 470                 475                 480 


Gly Gln Ala Asp Ala Leu Lys Gly Phe Ala Asp Gln Cys Met Ser Glu 
                485                 490                 495     


Tyr Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Phe Gly Pro Gly Asp 
            500                 505                 510         


Leu Pro Arg Pro Ala Thr Gly Ala 
        515                 520 


<210>  54
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SsPvcC-G315H+Tag mutant

<400>  54
atgacgaccg ctagtggtac caatgcagat gtccagaatg gcgtccgccc gatgacgggc       60

gccgaatatc tggattcgct gcgtgatggc cgtgccgtct atattcacgg cgaacgcgtc      120

cgcgatgtca ccgcgcatcc ggccttccgt aacagcgcgc gtagtctggc gcagctgtat      180

gatgtgctgc atgaaccgga ttcgcgtggc gttctgagcg tcccgaccga taccggtaat      240

ggcggtttta cgcacccgtt tttcaaaacc gcgcgtagcg ccggtgatct ggtggcagcc      300

cgcgatgcaa ttgtggcctg gcagcgtctt gtttacggtt ggatgggtcg tacgccggat      360

tataaagcag cattcttcgg tacgcttgaa gccaacgccg atttctatgg cccgttccgt      420

gataacgcac tggcatggta tcgtcgtgca caggaacgcg tgttgtattt caaccatgcg      480

atcgtgcatc cgccggtcga tcgcgatcgc ccggccgatc gcacggcgga tgtgtgcgtc      540

cacgtggaag aagaaacgga tgccggcctt gttgtttcgg gtgcgaaagt tgtcgcgacc      600

agcagcgccc tgacgaatgc caatctgatt gcacacatgg gcttaccgct gcgtgataaa      660

cgctttggcg ccatgttcac cgtgccgatg gatagcccgg gcctgaaact gttctgtcgt      720

accagttatg aaatgcatgc cgcggtctta ggctcgccgt ttgattaccc gttaagcagc      780

cgccttgatg aaaatgattc aatcatggtt cttgatcgtg ttttagtgcc gtgggaaaac      840

gtgtttatgt atgatgccgc cagcgcgaac gcgtttgcga cccgcagcgg tttcctggaa      900

cgttttacct ttcatggctg tacgcgctta gcggtgaaac tggattttat tgccggttgc      960

ctgctgaaag cagttgaagt gaccggcacc agccacttcc gtggtgtgca ggcccagatt     1020

ggcgaagttt taaactggcg cgatatgttt tggggtatgt cagatgcaat ggcgaaatcg     1080

ccgacggatt ggcacaatgg tgcggtccag ccgaatctga actatggcct ggcctaccgc     1140

accttcatgg gcattggtta cccgcgtatc cgtgaaatca ttcagcagac cattggctcg     1200

ggtctgattt atttaaatag tcacgcaagc gattggaaaa atccggaagt tcgtccgtac     1260

ttagatcgct acctgcgcgg tagtcgtggt gttgaagcga tcgatcgcgt taaactgctt     1320

aaactgctgt gggattgcgt gggcacggaa tttgcgggcc gtcacgaact ttatgaacgc     1380

aattacggtg gcgatcatga aggtattcgc gtgcagaccc tgttgagtta tcaggcgcgc     1440

ggtcaggcgg atgcgcttaa aggctttgcc gatcagtgta tgtcagaata cgatctggat     1500

ggctggaccc gcccggattt attcggtcca ggtgatttac ctagaccagc tactggagcg     1560

taa                                                                   1563


<210>  55
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SsPvcC-G315H+Tag mutant

<400>  55

Met Thr Thr Ala Ser Gly Thr Asn Ala Asp Val Gln Asn Gly Val Arg 
1               5                   10                  15      


Pro Met Thr Gly Ala Glu Tyr Leu Asp Ser Leu Arg Asp Gly Arg Ala 
            20                  25                  30          


Val Tyr Ile His Gly Glu Arg Val Arg Asp Val Thr Ala His Pro Ala 
        35                  40                  45              


Phe Arg Asn Ser Ala Arg Ser Leu Ala Gln Leu Tyr Asp Val Leu His 
    50                  55                  60                  


Glu Pro Asp Ser Arg Gly Val Leu Ser Val Pro Thr Asp Thr Gly Asn 
65                  70                  75                  80  


Gly Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ala Gly Asp 
                85                  90                  95      


Leu Val Ala Ala Arg Asp Ala Ile Val Ala Trp Gln Arg Leu Val Tyr 
            100                 105                 110         


Gly Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr 
        115                 120                 125             


Leu Glu Ala Asn Ala Asp Phe Tyr Gly Pro Phe Arg Asp Asn Ala Leu 
    130                 135                 140                 


Ala Trp Tyr Arg Arg Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala 
145                 150                 155                 160 


Ile Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Val Cys Val His Val Glu Glu Glu Thr Asp Ala Gly Leu Val Val 
            180                 185                 190         


Ser Gly Ala Lys Val Val Ala Thr Ser Ser Ala Leu Thr Asn Ala Asn 
        195                 200                 205             


Leu Ile Ala His Met Gly Leu Pro Leu Arg Asp Lys Arg Phe Gly Ala 
    210                 215                 220                 


Met Phe Thr Val Pro Met Asp Ser Pro Gly Leu Lys Leu Phe Cys Arg 
225                 230                 235                 240 


Thr Ser Tyr Glu Met His Ala Ala Val Leu Gly Ser Pro Phe Asp Tyr 
                245                 250                 255     


Pro Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Val Leu Asp 
            260                 265                 270         


Arg Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Ala Ser 
        275                 280                 285             


Ala Asn Ala Phe Ala Thr Arg Ser Gly Phe Leu Glu Arg Phe Thr Phe 
    290                 295                 300                 


His Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys 
305                 310                 315                 320 


Leu Leu Lys Ala Val Glu Val Thr Gly Thr Ser His Phe Arg Gly Val 
                325                 330                 335     


Gln Ala Gln Ile Gly Glu Val Leu Asn Trp Arg Asp Met Phe Trp Gly 
            340                 345                 350         


Met Ser Asp Ala Met Ala Lys Ser Pro Thr Asp Trp His Asn Gly Ala 
        355                 360                 365             


Val Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly 
    370                 375                 380                 


Ile Gly Tyr Pro Arg Ile Arg Glu Ile Ile Gln Gln Thr Ile Gly Ser 
385                 390                 395                 400 


Gly Leu Ile Tyr Leu Asn Ser His Ala Ser Asp Trp Lys Asn Pro Glu 
                405                 410                 415     


Val Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Val Glu 
            420                 425                 430         


Ala Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Cys Val Gly 
        435                 440                 445             


Thr Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly 
    450                 455                 460                 


Asp His Glu Gly Ile Arg Val Gln Thr Leu Leu Ser Tyr Gln Ala Arg 
465                 470                 475                 480 


Gly Gln Ala Asp Ala Leu Lys Gly Phe Ala Asp Gln Cys Met Ser Glu 
                485                 490                 495     


Tyr Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Phe Gly Pro Gly Asp 
            500                 505                 510         


Leu Pro Arg Pro Ala Thr Gly Ala 
        515                 520 


<210>  56
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SsPvcC-D214N+Tag mutant

<400>  56
atgacgaccg ctagtggtac caatgcagat gtccagaatg gcgtccgccc gatgacgggc       60

gccgaatatc tggattcgct gcgtgatggc cgtgccgtct atattcacgg cgaacgcgtc      120

cgcgatgtca ccgcgcatcc ggccttccgt aacagcgcgc gtagtctggc gcagctgtat      180

gatgtgctgc atgaaccgga ttcgcgtggc gttctgagcg tcccgaccga taccggtaat      240

ggcggtttta cgcacccgtt tttcaaaacc gcgcgtagcg ccggtgatct ggtggcagcc      300

cgcgatgcaa ttgtggcctg gcagcgtctt gtttacggtt ggatgggtcg tacgccggat      360

tataaagcag cattcttcgg tacgcttgaa gccaacgccg atttctatgg cccgttccgt      420

gataacgcac tggcatggta tcgtcgtgca caggaacgcg tgttgtattt caaccatgcg      480

atcgtgcatc cgccggtcga tcgcgatcgc ccggccgatc gcacggcgga tgtgtgcgtc      540

cacgtggaag aagaaacgga tgccggcctt gttgtttcgg gtgcgaaagt tgtcgcgacc      600

agcagcgccc tgacgaatgc caatctgatt gcacacatgg gcttaccgct gcgtgataaa      660

cgctttggcg ccatgttcac cgtgccgatg aatagcccgg gcctgaaact gttctgtcgt      720

accagttatg aaatgcatgc cgcggtctta ggctcgccgt ttgattaccc gttaagcagc      780

cgccttgatg aaaatgattc aatcatggtt cttgatcgtg ttttagtgcc gtgggaaaac      840

gtgtttatgt atgatgccgc cagcgcgaac gcgtttgcga cccgcagcgg tttcctggaa      900

cgttttacct ttcatggctg tacgcgctta gcggtgaaac tggattttat tgccggttgc      960

ctgctgaaag cagttgaagt gaccggcacc agccatttcc gtggtgtgca ggcccagatt     1020

ggcgaagttt taaactggcg cgatatgttt tggggtatgt cagatgcaat ggcgaaatcg     1080

ccgacggatt ggcacaatgg tgcggtccag ccgaatctga actatggcct ggcctaccgc     1140

accttcatgg gcattggtta cccgcgtatc cgtgaaatca ttcagcagac cattggctcg     1200

ggtctgattt atttaaatag tcacgcaagc gattggaaaa atccggaagt tcgtccgtac     1260

ttagatcgct acctgcgcgg tagtcgtggt gttgaagcga tcgatcgcgt taaactgctt     1320

aaactgctgt gggattgcgt gggcacggaa tttgcgggcc gtcacgaact ttatgaacgc     1380

aattacggtg gcgatcatga aggtattcgc gtgcagaccc tgttgagtta tcaggcgcgc     1440

ggtcaggcgg atgcgcttaa aggctttgcc gatcagtgta tgtcagaata cgatctggat     1500

ggctggaccc gcccggattt attcggtcca ggtgatttac ctagaccagc tactggagcg     1560

taa                                                                   1563


<210>  57
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SsPvcC-D214N+Tag mutant

<400>  57

Met Thr Thr Ala Ser Gly Thr Asn Ala Asp Val Gln Asn Gly Val Arg 
1               5                   10                  15      


Pro Met Thr Gly Ala Glu Tyr Leu Asp Ser Leu Arg Asp Gly Arg Ala 
            20                  25                  30          


Val Tyr Ile His Gly Glu Arg Val Arg Asp Val Thr Ala His Pro Ala 
        35                  40                  45              


Phe Arg Asn Ser Ala Arg Ser Leu Ala Gln Leu Tyr Asp Val Leu His 
    50                  55                  60                  


Glu Pro Asp Ser Arg Gly Val Leu Ser Val Pro Thr Asp Thr Gly Asn 
65                  70                  75                  80  


Gly Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ala Gly Asp 
                85                  90                  95      


Leu Val Ala Ala Arg Asp Ala Ile Val Ala Trp Gln Arg Leu Val Tyr 
            100                 105                 110         


Gly Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr 
        115                 120                 125             


Leu Glu Ala Asn Ala Asp Phe Tyr Gly Pro Phe Arg Asp Asn Ala Leu 
    130                 135                 140                 


Ala Trp Tyr Arg Arg Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala 
145                 150                 155                 160 


Ile Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Val Cys Val His Val Glu Glu Glu Thr Asp Ala Gly Leu Val Val 
            180                 185                 190         


Ser Gly Ala Lys Val Val Ala Thr Ser Ser Ala Leu Thr Asn Ala Asn 
        195                 200                 205             


Leu Ile Ala His Met Gly Leu Pro Leu Arg Asp Lys Arg Phe Gly Ala 
    210                 215                 220                 


Met Phe Thr Val Pro Met Asn Ser Pro Gly Leu Lys Leu Phe Cys Arg 
225                 230                 235                 240 


Thr Ser Tyr Glu Met His Ala Ala Val Leu Gly Ser Pro Phe Asp Tyr 
                245                 250                 255     


Pro Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Val Leu Asp 
            260                 265                 270         


Arg Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Ala Ser 
        275                 280                 285             


Ala Asn Ala Phe Ala Thr Arg Ser Gly Phe Leu Glu Arg Phe Thr Phe 
    290                 295                 300                 


His Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys 
305                 310                 315                 320 


Leu Leu Lys Ala Val Glu Val Thr Gly Thr Ser Gly Phe Arg Gly Val 
                325                 330                 335     


Gln Ala Gln Ile Gly Glu Val Leu Asn Trp Arg Asp Met Phe Trp Gly 
            340                 345                 350         


Met Ser Asp Ala Met Ala Lys Ser Pro Thr Asp Trp His Asn Gly Ala 
        355                 360                 365             


Val Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly 
    370                 375                 380                 


Ile Gly Tyr Pro Arg Ile Arg Glu Ile Ile Gln Gln Thr Ile Gly Ser 
385                 390                 395                 400 


Gly Leu Ile Tyr Leu Asn Ser His Ala Ser Asp Trp Lys Asn Pro Glu 
                405                 410                 415     


Val Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Val Glu 
            420                 425                 430         


Ala Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Cys Val Gly 
        435                 440                 445             


Thr Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly 
    450                 455                 460                 


Asp His Glu Gly Ile Arg Val Gln Thr Leu Leu Ser Tyr Gln Ala Arg 
465                 470                 475                 480 


Gly Gln Ala Asp Ala Leu Lys Gly Phe Ala Asp Gln Cys Met Ser Glu 
                485                 490                 495     


Tyr Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Phe Gly Pro Gly Asp 
            500                 505                 510         


Leu Pro Arg Pro Ala Thr Gly Ala 
        515                 520 


<210>  58
<211>  1563
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SsPvcC+Tag

<400>  58
atgacgaccg ctagtggtac caatgcagat gtccagaatg gcgtccgccc gatgacgggc       60

gccgaatatc tggattcgct gcgtgatggc cgtgccgtct atattcacgg cgaacgcgtc      120

cgcgatgtca ccgcgcatcc ggccttccgt aacagcgcgc gtagtctggc gcagctgtat      180

gatgtgctgc atgaaccgga ttcgcgtggc gttctgagcg tcccgaccga taccggtaat      240

ggcggtttta cgcacccgtt tttcaaaacc gcgcgtagcg ccggtgatct ggtggcagcc      300

cgcgatgcaa ttgtggcctg gcagcgtctt gtttacggtt ggatgggtcg tacgccggat      360

tataaagcag cattcttcgg tacgcttgaa gccaacgccg atttctatgg cccgttccgt      420

gataacgcac tggcatggta tcgtcgtgca caggaacgcg tgttgtattt caaccatgcg      480

atcgtgcatc cgccggtcga tcgcgatcgc ccggccgatc gcacggcgga tgtgtgcgtc      540

cacgtggaag aagaaacgga tgccggcctt gttgtttcgg gtgcgaaagt tgtcgcgacc      600

agcagcgccc tgacgaatgc caatctgatt gcacacatgg gcttaccgct gcgtgataaa      660

cgctttggcg ccatgttcac cgtgccgatg gatagcccgg gcctgaaact gttctgtcgt      720

accagttatg aaatgcatgc cgcggtctta ggctcgccgt ttgattaccc gttaagcagc      780

cgccttgatg aaaatgattc aatcatggtt cttgatcgtg ttttagtgcc gtgggaaaac      840

gtgtttatgt atgatgccgc cagcgcgaac gcgtttgcga cccgcagcgg tttcctggaa      900

cgttttacct ttcatggctg tacgcgctta gcggtgaaac tggattttat tgccggttgc      960

ctgctgaaag cagttgaagt gaccggcacc agcggcttcc gtggtgtgca ggcccagatt     1020

ggcgaagttt taaactggcg cgatatgttt tggggtatgt cagatgcaat ggcgaaatcg     1080

ccgacggatt ggcacaatgg tgcggtccag ccgaatctga actatggcct ggcctaccgc     1140

accttcatgg gcattggtta cccgcgtatc cgtgaaatca ttcagcagac cattggctcg     1200

ggtctgattt atttaaatag tcacgcaagc gattggaaaa atccggaagt tcgtccgtac     1260

ttagatcgct acctgcgcgg tagtcgtggt gttgaagcga tcgatcgcgt taaactgctt     1320

aaactgctgt gggattgcgt gggcacggaa tttgcgggcc gtcacgaact ttatgaacgc     1380

aattacggtg gcgatcatga aggtattcgc gtgcagaccc tgttgagtta tcaggcgcgc     1440

ggtcaggcgg atgcgcttaa aggctttgcc gatcagtgta tgtcagaata cgatctggat     1500

ggctggaccc gcccggattt attcggtcca ggtgatttac ctagaccagc tactggagcg     1560

taa                                                                   1563


<210>  59
<211>  520
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SsPvcC+Tag

<400>  59

Met Thr Thr Ala Ser Gly Thr Asn Ala Asp Val Gln Asn Gly Val Arg 
1               5                   10                  15      


Pro Met Thr Gly Ala Glu Tyr Leu Asp Ser Leu Arg Asp Gly Arg Ala 
            20                  25                  30          


Val Tyr Ile His Gly Glu Arg Val Arg Asp Val Thr Ala His Pro Ala 
        35                  40                  45              


Phe Arg Asn Ser Ala Arg Ser Leu Ala Gln Leu Tyr Asp Val Leu His 
    50                  55                  60                  


Glu Pro Asp Ser Arg Gly Val Leu Ser Val Pro Thr Asp Thr Gly Asn 
65                  70                  75                  80  


Gly Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ala Gly Asp 
                85                  90                  95      


Leu Val Ala Ala Arg Asp Ala Ile Val Ala Trp Gln Arg Leu Val Tyr 
            100                 105                 110         


Gly Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr 
        115                 120                 125             


Leu Glu Ala Asn Ala Asp Phe Tyr Gly Pro Phe Arg Asp Asn Ala Leu 
    130                 135                 140                 


Ala Trp Tyr Arg Arg Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala 
145                 150                 155                 160 


Ile Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Val Cys Val His Val Glu Glu Glu Thr Asp Ala Gly Leu Val Val 
            180                 185                 190         


Ser Gly Ala Lys Val Val Ala Thr Ser Ser Ala Leu Thr Asn Ala Asn 
        195                 200                 205             


Leu Ile Ala His Met Gly Leu Pro Leu Arg Asp Lys Arg Phe Gly Ala 
    210                 215                 220                 


Met Phe Thr Val Pro Met Asp Ser Pro Gly Leu Lys Leu Phe Cys Arg 
225                 230                 235                 240 


Thr Ser Tyr Glu Met His Ala Ala Val Leu Gly Ser Pro Phe Asp Tyr 
                245                 250                 255     


Pro Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Val Leu Asp 
            260                 265                 270         


Arg Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Ala Ser 
        275                 280                 285             


Ala Asn Ala Phe Ala Thr Arg Ser Gly Phe Leu Glu Arg Phe Thr Phe 
    290                 295                 300                 


His Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys 
305                 310                 315                 320 


Leu Leu Lys Ala Val Glu Val Thr Gly Thr Ser Gly Phe Arg Gly Val 
                325                 330                 335     


Gln Ala Gln Ile Gly Glu Val Leu Asn Trp Arg Asp Met Phe Trp Gly 
            340                 345                 350         


Met Ser Asp Ala Met Ala Lys Ser Pro Thr Asp Trp His Asn Gly Ala 
        355                 360                 365             


Val Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly 
    370                 375                 380                 


Ile Gly Tyr Pro Arg Ile Arg Glu Ile Ile Gln Gln Thr Ile Gly Ser 
385                 390                 395                 400 


Gly Leu Ile Tyr Leu Asn Ser His Ala Ser Asp Trp Lys Asn Pro Glu 
                405                 410                 415     


Val Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Val Glu 
            420                 425                 430         


Ala Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Cys Val Gly 
        435                 440                 445             


Thr Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly 
    450                 455                 460                 


Asp His Glu Gly Ile Arg Val Gln Thr Leu Leu Ser Tyr Gln Ala Arg 
465                 470                 475                 480 


Gly Gln Ala Asp Ala Leu Lys Gly Phe Ala Asp Gln Cys Met Ser Glu 
                485                 490                 495     


Tyr Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Phe Gly Pro Gly Asp 
            500                 505                 510         


Leu Pro Arg Pro Ala Thr Gly Ala 
        515                 520 


<210>  60
<211>  1512
<212>  DNA
<213>  Streptomyces sclerotialus

<400>  60
atgacgggcg ccgaatatct ggattcgctg cgtgatggcc gtgccgtcta tattcacggc       60

gaacgcgtcc gcgatgtcac cgcgcatccg gccttccgta acagcgcgcg tagtctggcg      120

cagctgtatg atgtgctgca tgaaccggat tcgcgtggcg ttctgagcgt cccgaccgat      180

accggtaatg gcggttttac gcacccgttt ttcaaaaccg cgcgtagcgc cggtgatctg      240

gtggcagccc gcgatgcaat tgtggcctgg cagcgtcttg tttacggttg gatgggtcgt      300

acgccggatt ataaagcagc attcttcggt acgcttgaag ccaacgccga tttctatggc      360

ccgttccgtg ataacgcact ggcatggtat cgtcgtgcac aggaacgcgt gttgtatttc      420

aaccatgcga tcgtgcatcc gccggtcgat cgcgatcgcc cggccgatcg cacggcggat      480

gtgtgcgtcc acgtggaaga agaaacggat gccggccttg ttgtttcggg tgcgaaagtt      540

gtcgcgacca gcagcgccct gacgaatgcc aatctgattg cacacatggg cttaccgctg      600

cgtgataaac gctttggcgc catgttcacc gtgccgatgg atagcccggg cctgaaactg      660

ttctgtcgta ccagttatga aatgcatgcc gcggtcttag gctcgccgtt tgattacccg      720

ttaagcagcc gccttgatga aaatgattca atcatggttc ttgatcgtgt tttagtgccg      780

tgggaaaacg tgtttatgta tgatgccgcc agcgcgaacg cgtttgcgac ccgcagcggt      840

ttcctggaac gttttacctt tcatggctgt acgcgcttag cggtgaaact ggattttatt      900

gccggttgcc tgctgaaagc agttgaagtg accggcacca gcggcttccg tggtgtgcag      960

gcccagattg gcgaagtttt aaactggcgc gatatgtttt ggggtatgtc agatgcaatg     1020

gcgaaatcgc cgacggattg gcacaatggt gcggtccagc cgaatctgaa ctatggcctg     1080

gcctaccgca ccttcatggg cattggttac ccgcgtatcc gtgaaatcat tcagcagacc     1140

attggctcgg gtctgattta tttaaatagt cacgcaagcg attggaaaaa tccggaagtt     1200

cgtccgtact tagatcgcta cctgcgcggt agtcgtggtg ttgaagcgat cgatcgcgtt     1260

aaactgctta aactgctgtg ggattgcgtg ggcacggaat ttgcgggccg tcacgaactt     1320

tatgaacgca attacggtgg cgatcatgaa ggtattcgcg tgcagaccct gttgagttat     1380

caggcgcgcg gtcaggcgga tgcgcttaaa ggctttgccg atcagtgtat gtcagaatac     1440

gatctggatg gctggacccg cccggattta ttcggtccag gtgatttacc tagaccagct     1500

actggagcgt aa                                                         1512


<210>  61
<211>  503
<212>  PRT
<213>  Streptomyces sclerotialus

<400>  61

Met Thr Gly Ala Glu Tyr Leu Asp Ser Leu Arg Asp Gly Arg Ala Val 
1               5                   10                  15      


Tyr Ile His Gly Glu Arg Val Arg Asp Val Thr Ala His Pro Ala Phe 
            20                  25                  30          


Arg Asn Ser Ala Arg Ser Leu Ala Gln Leu Tyr Asp Val Leu His Glu 
        35                  40                  45              


Pro Asp Ser Arg Gly Val Leu Ser Val Pro Thr Asp Thr Gly Asn Gly 
    50                  55                  60                  


Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ala Gly Asp Leu 
65                  70                  75                  80  


Val Ala Ala Arg Asp Ala Ile Val Ala Trp Gln Arg Leu Val Tyr Gly 
                85                  90                  95      


Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr Leu 
            100                 105                 110         


Glu Ala Asn Ala Asp Phe Tyr Gly Pro Phe Arg Asp Asn Ala Leu Ala 
        115                 120                 125             


Trp Tyr Arg Arg Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala Ile 
    130                 135                 140                 


Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala Asp 
145                 150                 155                 160 


Val Cys Val His Val Glu Glu Glu Thr Asp Ala Gly Leu Val Val Ser 
                165                 170                 175     


Gly Ala Lys Val Val Ala Thr Ser Ser Ala Leu Thr Asn Ala Asn Leu 
            180                 185                 190         


Ile Ala His Met Gly Leu Pro Leu Arg Asp Lys Arg Phe Gly Ala Met 
        195                 200                 205             


Phe Thr Val Pro Met Asp Ser Pro Gly Leu Lys Leu Phe Cys Arg Thr 
    210                 215                 220                 


Ser Tyr Glu Met His Ala Ala Val Leu Gly Ser Pro Phe Asp Tyr Pro 
225                 230                 235                 240 


Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Val Leu Asp Arg 
                245                 250                 255     


Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Ala Ser Ala 
            260                 265                 270         


Asn Ala Phe Ala Thr Arg Ser Gly Phe Leu Glu Arg Phe Thr Phe His 
        275                 280                 285             


Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys Leu 
    290                 295                 300                 


Leu Lys Ala Val Glu Val Thr Gly Thr Ser Gly Phe Arg Gly Val Gln 
305                 310                 315                 320 


Ala Gln Ile Gly Glu Val Leu Asn Trp Arg Asp Met Phe Trp Gly Met 
                325                 330                 335     


Ser Asp Ala Met Ala Lys Ser Pro Thr Asp Trp His Asn Gly Ala Val 
            340                 345                 350         


Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly Ile 
        355                 360                 365             


Gly Tyr Pro Arg Ile Arg Glu Ile Ile Gln Gln Thr Ile Gly Ser Gly 
    370                 375                 380                 


Leu Ile Tyr Leu Asn Ser His Ala Ser Asp Trp Lys Asn Pro Glu Val 
385                 390                 395                 400 


Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Val Glu Ala 
                405                 410                 415     


Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Cys Val Gly Thr 
            420                 425                 430         


Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly Asp 
        435                 440                 445             


His Glu Gly Ile Arg Val Gln Thr Leu Leu Ser Tyr Gln Ala Arg Gly 
    450                 455                 460                 


Gln Ala Asp Ala Leu Lys Gly Phe Ala Asp Gln Cys Met Ser Glu Tyr 
465                 470                 475                 480 


Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Phe Gly Pro Gly Asp Leu 
                485                 490                 495     


Pro Arg Pro Ala Thr Gly Ala 
            500             


<210>  62
<211>  1539
<212>  DNA
<213>  Saccharothrix espanaensis

<400>  62
atgacgatta cctctccggc cccggctggt cgcctgaaca atgtgcgtcc gatgacgggt       60

gaagaatacc tggaatccct gcgtgacggt cgtgaagtgt atatttacgg cgaacgcgtc      120

gatgacgtga ccacgcatct ggcgttccgc aacagcgttc gttctatcgc ccgcctgtat      180

gatgtcctgc acgatccggc ctccgaaggt gttctgcgcg tcccgaccga taccggtaat      240

ggtggtttta cccatccgtt tttcaaaacg gcgcgtagct ctgaagacct ggtggcggcc      300

cgtgaagcca ttgtcggttg gcaacgcctg gtgtatggct ggatgggtcg taccccggat      360

tacaaggcag cgtttttcgg tacgctggac gctaacgcgg aattttatgg cccgttcgaa      420

gccaatgcac gtcgctggta tcgtgatgca caggaacgcg ttctgtactt caaccatgct      480

atcgtgcatc cgccggtcga tcgtgaccgt ccggctgatc gtaccgccga catttgcgtc      540

catgtggaag aagaaacgga ttcaggcctg atcgtgtcgg gtgccaaagt ggttgcaacc      600

ggttctgcta tgacgaacgc gaatctgatt gcccactatg gtctgccggt tcgcgataaa      660

aagtttggcc tggtgttcac cgttccgatg aacagtccgg gtctgaaact gatctgtcgt      720

acctcctatg aactgatggt ggccacgcag ggctcaccgt ttgattaccc gctgagttcc      780

cgcctggatg aaaatgacag cattatgatc tttgatcgtg ttctggtccc gtgggaaaac      840

gttttcatgt acgacgcagg cgcggccaat agctttgcta ccggctctgg tttcctggaa      900

cgctttacct ttcatggctg cacgcgtctg gcagtgaaac tggattttat tgcaggctgt      960

gttatgaagg ctgtggaagt taccggcacc acgcacttcc gcggtgttca ggcgcaagtc     1020

ggcgaagtgc tgaactggcg tgatgtcttt tggggtctgt cggacgctat ggcgaaaagt     1080

ccgaacagct gggtgggcgg tagcgttcag ccgaacctga attatggcct ggcctaccgc     1140

acctttatgg gcgtgggtta tccgcgtatt aaagaaatta tccagcaaac gctgggctct     1200

ggtctgatct acctgaactc atcggcagct gattggaaga atccggacgt tcgcccgtat     1260

ctggatcgtt acctgcgcgg cagtcgtggt attcaggcaa tcgatcgtgt caaactgctg     1320

aagctgctgt gggacgcggt gggcaccgaa tttgccggtc gtcatgaact gtatgaacgc     1380

aactacggcg gtgatcacga aggcattcgt gtgcagaccc tgcaagccta tcaggcaaat     1440

ggtcaagcgg cggcactgaa aggctttgcg gaacagtgca tgagcgaata cgacctggat     1500

ggctggaccc gcccggacct gattaacccg ggcacctga                            1539


<210>  63
<211>  512
<212>  PRT
<213>  Saccharothrix espanaensis

<400>  63

Met Thr Ile Thr Ser Pro Ala Pro Ala Gly Arg Leu Asn Asn Val Arg 
1               5                   10                  15      


Pro Met Thr Gly Glu Glu Tyr Leu Glu Ser Leu Arg Asp Gly Arg Glu 
            20                  25                  30          


Val Tyr Ile Tyr Gly Glu Arg Val Asp Asp Val Thr Thr His Leu Ala 
        35                  40                  45              


Phe Arg Asn Ser Val Arg Ser Ile Ala Arg Leu Tyr Asp Val Leu His 
    50                  55                  60                  


Asp Pro Ala Ser Glu Gly Val Leu Arg Val Pro Thr Asp Thr Gly Asn 
65                  70                  75                  80  


Gly Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ser Glu Asp 
                85                  90                  95      


Leu Val Ala Ala Arg Glu Ala Ile Val Gly Trp Gln Arg Leu Val Tyr 
            100                 105                 110         


Gly Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr 
        115                 120                 125             


Leu Asp Ala Asn Ala Glu Phe Tyr Gly Pro Phe Glu Ala Asn Ala Arg 
    130                 135                 140                 


Arg Trp Tyr Arg Asp Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala 
145                 150                 155                 160 


Ile Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Ile Cys Val His Val Glu Glu Glu Thr Asp Ser Gly Leu Ile Val 
            180                 185                 190         


Ser Gly Ala Lys Val Val Ala Thr Gly Ser Ala Met Thr Asn Ala Asn 
        195                 200                 205             


Leu Ile Ala His Tyr Gly Leu Pro Val Arg Asp Lys Lys Phe Gly Leu 
    210                 215                 220                 


Val Phe Thr Val Pro Met Asn Ser Pro Gly Leu Lys Leu Ile Cys Arg 
225                 230                 235                 240 


Thr Ser Tyr Glu Leu Met Val Ala Thr Gln Gly Ser Pro Phe Asp Tyr 
                245                 250                 255     


Pro Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Ile Phe Asp 
            260                 265                 270         


Arg Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Gly Ala 
        275                 280                 285             


Ala Asn Ser Phe Ala Thr Gly Ser Gly Phe Leu Glu Arg Phe Thr Phe 
    290                 295                 300                 


His Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys 
305                 310                 315                 320 


Val Met Lys Ala Val Glu Val Thr Gly Thr Thr His Phe Arg Gly Val 
                325                 330                 335     


Gln Ala Gln Val Gly Glu Val Leu Asn Trp Arg Asp Val Phe Trp Gly 
            340                 345                 350         


Leu Ser Asp Ala Met Ala Lys Ser Pro Asn Ser Trp Val Gly Gly Ser 
        355                 360                 365             


Val Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly 
    370                 375                 380                 


Val Gly Tyr Pro Arg Ile Lys Glu Ile Ile Gln Gln Thr Leu Gly Ser 
385                 390                 395                 400 


Gly Leu Ile Tyr Leu Asn Ser Ser Ala Ala Asp Trp Lys Asn Pro Asp 
                405                 410                 415     


Val Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Ile Gln 
            420                 425                 430         


Ala Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Ala Val Gly 
        435                 440                 445             


Thr Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly 
    450                 455                 460                 


Asp His Glu Gly Ile Arg Val Gln Thr Leu Gln Ala Tyr Gln Ala Asn 
465                 470                 475                 480 


Gly Gln Ala Ala Ala Leu Lys Gly Phe Ala Glu Gln Cys Met Ser Glu 
                485                 490                 495     


Tyr Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Ile Asn Pro Gly Thr 
            500                 505                 510         


<210>  64
<211>  510
<212>  DNA
<213>  Saccharothrix espanaensis

<400>  64
atgatgaccg tttatgatag cgcactgaca atggaagaaa ccaccctgcg tgatgcaatg       60

agccgttttg caaccggtgt tagcgttgtt accgttggtg gtgaacatac acatggtatg      120

accgcaaatg cctttacctg tgttagcctg gatccgcctc tggttctgtg ttgtgttgca      180

cgtaaagcaa ccatgcatgc agcaattgaa ggtgcacgtc gttttgcagt tagcgttatg      240

ggtggtgatc aagaacgtac cgcacgttat tttgcagata aacgtcgtcc gcgtggtcgt      300

gcacagtttg atgttgttga ttggcagcct ggtccgcata caggtgcacc gctgctgagc      360

ggtgcgctgg catggctgga atgtgaagtt gcacagtggc atgaaggtgg cgatcatacc      420

atttttctgg gtcgtgttct gggttgtcgt cgtggtccgg atagtccggc actgctgttt      480

tatggtagcg attttcatca gatccgctaa                                       510


<210>  65
<211>  169
<212>  PRT
<213>  Saccharothrix espanaensis

<400>  65

Met Met Thr Val Tyr Asp Ser Ala Leu Thr Met Glu Glu Thr Thr Leu 
1               5                   10                  15      


Arg Asp Ala Met Ser Arg Phe Ala Thr Gly Val Ser Val Val Thr Val 
            20                  25                  30          


Gly Gly Glu His Thr His Gly Met Thr Ala Asn Ala Phe Thr Cys Val 
        35                  40                  45              


Ser Leu Asp Pro Pro Leu Val Leu Cys Cys Val Ala Arg Lys Ala Thr 
    50                  55                  60                  


Met His Ala Ala Ile Glu Gly Ala Arg Arg Phe Ala Val Ser Val Met 
65                  70                  75                  80  


Gly Gly Asp Gln Glu Arg Thr Ala Arg Tyr Phe Ala Asp Lys Arg Arg 
                85                  90                  95      


Pro Arg Gly Arg Ala Gln Phe Asp Val Val Asp Trp Gln Pro Gly Pro 
            100                 105                 110         


His Thr Gly Ala Pro Leu Leu Ser Gly Ala Leu Ala Trp Leu Glu Cys 
        115                 120                 125             


Glu Val Ala Gln Trp His Glu Gly Gly Asp His Thr Ile Phe Leu Gly 
    130                 135                 140                 


Arg Val Leu Gly Cys Arg Arg Gly Pro Asp Ser Pro Ala Leu Leu Phe 
145                 150                 155                 160 


Tyr Gly Ser Asp Phe His Gln Ile Arg 
                165                 


<210>  66
<211>  561
<212>  DNA
<213>  Pseudomonas fluorescens

<400>  66
atgaatgcag caaccgaaac caaagttcat gatctgctgg atgccgaagg tcgtgatgtt       60

cgtgatgcac gtgaactgcg taatgttctg ggtcagtttg caaccggtgt taccgttatt      120

accacccgta ccgcagatgg tcgtaatgtt ggtgtgaccg caaatagctt tagcagcctg      180

agcctgagtc cggcactggt tctgtggtca ctggcacgta ccgcaccgag cctgaaagtt      240

ttttgtagcg caagccattt tgccattaat gtgctgggtg cacatcagct gcatctgagc      300

gaacagtttg cacgtgccgc agcagataaa tttgccggtg ttgcacatag ttatggtaaa      360

gcgggtgcac cggttctgga tgatgttgtt gcagttctgg tttgccgtaa tgttacccag      420

tatgaaggtg gtgatcatct gatttttatc ggcgaaattg agcagtatcg ttatagcggt      480

gcagaaccgc tggtttttca tgcaggtcag tatcgtggtc tgggtagcaa tcgtgcagaa      540

agcgttctga aacatgaata a                                                561


<210>  67
<211>  186
<212>  PRT
<213>  Pseudomonas fluorescens

<400>  67

Met Asn Ala Ala Thr Glu Thr Lys Val His Asp Leu Leu Asp Ala Glu 
1               5                   10                  15      


Gly Arg Asp Val Arg Asp Ala Arg Glu Leu Arg Asn Val Leu Gly Gln 
            20                  25                  30          


Phe Ala Thr Gly Val Thr Val Ile Thr Thr Arg Thr Ala Asp Gly Arg 
        35                  40                  45              


Asn Val Gly Val Thr Ala Asn Ser Phe Ser Ser Leu Ser Leu Ser Pro 
    50                  55                  60                  


Ala Leu Val Leu Trp Ser Leu Ala Arg Thr Ala Pro Ser Leu Lys Val 
65                  70                  75                  80  


Phe Cys Ser Ala Ser His Phe Ala Ile Asn Val Leu Gly Ala His Gln 
                85                  90                  95      


Leu His Leu Ser Glu Gln Phe Ala Arg Ala Ala Ala Asp Lys Phe Ala 
            100                 105                 110         


Gly Val Ala His Ser Tyr Gly Lys Ala Gly Ala Pro Val Leu Asp Asp 
        115                 120                 125             


Val Val Ala Val Leu Val Cys Arg Asn Val Thr Gln Tyr Glu Gly Gly 
    130                 135                 140                 


Asp His Leu Ile Phe Ile Gly Glu Ile Glu Gln Tyr Arg Tyr Ser Gly 
145                 150                 155                 160 


Ala Glu Pro Leu Val Phe His Ala Gly Gln Tyr Arg Gly Leu Gly Ser 
                165                 170                 175     


Asn Arg Ala Glu Ser Val Leu Lys His Glu 
            180                 185     


<210>  68
<211>  513
<212>  DNA
<213>  Escherichia coli

<400>  68
atgcaattag atgaacaacg cctgcgcttt cgtgacgcaa tggccagcct gtcggcagcg       60

gtaaatatta tcaccaccga gggcgacgcc ggacaatgcg ggattacggc aacggccgtc      120

tgctcggtca cggatacacc accatcgctg atggtgtgca ttaacgccaa cagtgcgatg      180

aacccggttt ttcagggcaa cggtaagttg tgcgtcaacg tcctcaacca tgagcaggaa      240

ctgatggcac gccacttcgc gggcatgaca ggcatggcga tggaagagcg ttttagcctc      300

tcatgctggc aaaaaggtcc gctggcgcag ccggtgctaa aaggttcgct ggccagtctt      360

gaaggtgaga tccgcgatgt gcaggcaatt ggcacacatc tggtgtatct ggtggagatt      420

aaaaacatca tcctcagtgc agaaggtcac ggacttatct actttaaacg ccgtttccat      480

ccggtgatgc tggaaatgga agctgcgatt taa                                   513


<210>  69
<211>  170
<212>  PRT
<213>  Escherichia coli

<400>  69

Met Gln Leu Asp Glu Gln Arg Leu Arg Phe Arg Asp Ala Met Ala Ser 
1               5                   10                  15      


Leu Ser Ala Ala Val Asn Ile Ile Thr Thr Glu Gly Asp Ala Gly Gln 
            20                  25                  30          


Cys Gly Ile Thr Ala Thr Ala Val Cys Ser Val Thr Asp Thr Pro Pro 
        35                  40                  45              


Ser Leu Met Val Cys Ile Asn Ala Asn Ser Ala Met Asn Pro Val Phe 
    50                  55                  60                  


Gln Gly Asn Gly Lys Leu Cys Val Asn Val Leu Asn His Glu Gln Glu 
65                  70                  75                  80  


Leu Met Ala Arg His Phe Ala Gly Met Thr Gly Met Ala Met Glu Glu 
                85                  90                  95      


Arg Phe Ser Leu Ser Cys Trp Gln Lys Gly Pro Leu Ala Gln Pro Val 
            100                 105                 110         


Leu Lys Gly Ser Leu Ala Ser Leu Glu Gly Glu Ile Arg Asp Val Gln 
        115                 120                 125             


Ala Ile Gly Thr His Leu Val Tyr Leu Val Glu Ile Lys Asn Ile Ile 
    130                 135                 140                 


Leu Ser Ala Glu Gly His Gly Leu Ile Tyr Phe Lys Arg Arg Phe His 
145                 150                 155                 160 


Pro Val Met Leu Glu Met Glu Ala Ala Ile 
                165                 170 


<210>  70
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal tag

<400>  70

Met Thr Thr Ala Ser Gly Thr Asn Ala Asp Val Gln Asn Gly Val Arg 
1               5                   10                  15      


Pro 
    


