                         SEQUENCE LISTING

<110>  President and Fellows of Harvard College
 
<120>  USES OF MODIFIED RNA ENCODING RETINALDEHYDE DEHYDROGENASE

<130>  H0824.70294WO00

<140>  Not yet assigned
<141>  2019-10-10

<150>  US 62/743,943
<151>  2018-10-10

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1506
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  1
augucuucgc cugcacaacc ugcagucccu gccccacugg ccgacuugaa gauucaacau       60

accaagaucu ucauaaacaa ugaauggcac aauucaguga gcggcaagaa auuuccaguu      120

cuuaacccug caacugagga ggucaucugc cacguggaag aaggggacaa ggcugauguu      180

gacaaagcug ugaaggcugc aagacaggcu uuccagauug gcucuccaug gcgcaccaug      240

gaugcuucag agaggggccg ccugcugaac aagcuggcug acuuaaugga gagagaucgu      300

cugcugcugg cuacaaugga ggcacucaau ggugggaaag ucuuugccaa ugcauacuug      360

ucggauuuag gaggcugcau aaaagcauua aaguacugug caggcugggc ugacaagauu      420

cauggucaaa caauaccaag ugauggagac auuuucacuu auacaagacg ugaaccuauu      480

ggagugugug gccaaaucau ccccuggaau uuuccaaugc ucauguucau uuggaagaua      540

ggcccugccc uuagcugugg gaauaccgug guugucaagc cagcagagca aacuccucuc      600

acggcucuuc accuggcauc uuuaauaaaa gaggcagggu uuccuccugg cgugguaaac      660

auugucccug guuaugggcc aacugcaggg gcagccaucu ccucucacau ggaugucgac      720

aagguggccu ucacuggauc aacacagguu ggcaaguuaa ucaaggaagc ugcagggaaa      780

agcaaucuga agagagucac ccuggagcug gggggaaaga gcccuugcau uguguuugca      840

gaugccgacu uggacauugc uguugaguuu gcacaccaug gaguguuuua ucaucaaggc      900

caauguugug ucgcagcauc ccggauuuuu guugaggagu caguuuauga ugaguuugug      960

aaaaggagug uugagcgagc uaagaaauau guucuuggaa auccucugac cccaggaaua     1020

aaucaaggcc cucagauuga caaggaacaa caugauaaaa uacucgaucu cauugagagu     1080

gggaagaaag aaggagccaa acuggagugu gguggaggac gcugggggaa caaaggcuuc     1140

uuugugcagc ccacaguguu cuccaacgug acugaugaga ugcgcauugc caaagaggag     1200

auauuuggac cagugcaaca aaucaugaag uuuaagucug uagaugaugu gaucaagaga     1260

gcaaacaaua cuaccuaugg uuuagcagca ggacucuuca cuaaagaccu ggauaaggcc     1320

aucacugugu caucugcucu gcaggcuggg gugguguggg uuaacugcua uaugauguug     1380

ucagcccagu gccccuucgg uggauucaag augucuggaa auggaagaga acugggugaa     1440

cauggucuuu augaauacac ugagcucaag acagucgcaa ugaagauauc ucagaagaac     1500

uccuaa                                                                1506


<210>  2
<211>  1557
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  2
augacuucca gcgagaucgc caugccgggc gaggugaagg ccgaccccgc cgcgcucaug       60

gccucgcugc agcuccugcc gucgcccacg cccaaccucg agaucaagua caccaagauc      120

uuuauuaaca augaauggca gaacucagag agugggagag uguucccugu cuguaaucca      180

gccacaggag agcaagugug ugaaguucaa gaagcagaca agguggauau agacaaggca      240

gugcaagcag cccgccuugc cuucucucuu gguucugugu ggagaaggau ggaugcgucu      300

gaaagaggac gucuguugga caagcuugca gacuuggugg aacgggacag ggcaacucuu      360

gcaacuaugg aaucccuaaa uggcgguaag ccauuccugc aagcuuuuua caucgauuug      420

cagggaguca ucaaaacccu gagguauuau gcaggcuggg cugauaaaau ucacggaaug      480

accauuccug uagauggaga cuauuuuacu uucacaagac acgagcccau uggagugugu      540

ggacagauca ucccguggaa cuucccccug cugauguuca ccuggaaaau ugcucccgca      600

uugugcugug guaacaccgu ggucaucaaa cccgcugagc agacaccgcu cagugcacug      660

uauaugggag cccucaucaa ggaggcuggc uuuccacccg gagucgucaa uauccugcca      720

ggguaugggc caacagcagg ggcagcaauc gcuucucaca ucggcauaga caagauugca      780

uucacaggcu cuacugaggu uggaaagcuu auucaagaag cagcuggaag aaguaaccug      840

aagagaguga cccuggaacu cggagggaag agcccuaaua uuauuuuugc agaugcugac      900

uuggacuacg cuguggagca ggcucaccag gguguguucu ucaaccaagg ccagugcugc      960

accgcagggu cucgcaucuu uguggaagaa uccaucuaug aggaauuugu gaaaagaagu     1020

guggaaaggg ccaagaggcg cauugugggg agccccuuug aucccacaac ugagcagggu     1080

ccucagaucg auaagaaaca auacaacaaa guccuggagc uuauccagag cgguguggcu     1140

gagggcgcca agcuggagug cggaggcaaa gggcugggaa ggaagggcuu cuucauugag     1200

cccaccgugu ucuccaacgu cacugaugac augcggauug ccaaggagga gaucuuuggu     1260

ccuguucaag aaauuuugag guuuaagacu auggaugaag uuauagaaag agccaauaac     1320

ucagacuuug gacuuguagc agcugucuuc acuaaugaca ucaacaaggc ucucauggua     1380

uccuccgcaa ugcaagcugg gacaguuugg aucaauuguu acaaugccuu aaaugcgcag     1440

agccccuuug ggggguucaa gaugucugga aaugggagag aaauggguga guuuggcuua     1500

cgggaguauu cagaaguaaa gaccgugacg gugaagaucc cccagaagaa cuccuaa        1557


<210>  3
<211>  1539
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  3
auggcuacca ccaacggggc uguggaaaac ggacagccgg augggaaacc gccugccuug       60

ccgcgcccca uccgcaacuu ggaggucaag uucaccaaga uauuuaucaa caacgacugg      120

cacgaaucca agaguggaag aaaguuugcc acauauaacc cuucaacacu agagaaaaua      180

ugugaggugg aagaaggaga uaagcccgau guggacaagg cuguggaggc cgcucaagcu      240

gccuuccagc ggggaucccc guggcgccgg cuggaugcac ugagcagagg ccaguugcug      300

caucagcugg cugaccuugu agaaagggac cgagcgaucc uggcuacucu ggagaccaug      360

gacaccggca agccauuccu ucaugccuuu uucgucgacc uggaaggcug uauuaagacc      420

uucagauauu uugccgggug ggcagacaaa auccagggca ggaccauccc cacagaugac      480

aacguugugu gcuucaccag gcaugagccc aucggggugu guggggccau uacaccaugg      540

aacuuccccc ugcugaugcu ggccuggaaa cuggcuccug cccugugcug ugggaacacc      600

gugguccuga agccagcuga gcagaccccu cucacggcuc uguaccuagc cucucucauc      660

aaagaggucg gguucccucc ggguguggug aacauuguac caggcuuugg gcccacugug      720

ggagcagcaa uuuccuccca uccgcagauc aacaagauag ccuucaccgg cuccacagag      780

guuggaaagc uggucagaga agccgccucc cggagcaacc ugaagagggu cacacuggag      840

cuaggaggca agaacccgug caucgugugu gcagaugcug acuuggacuu ggccgucgag      900

ugugcucacc agggaguguu cuucaaccaa ggccagugcu guacagcggc cuccagggug      960

uucguggaag agcaggucua cggggaguuu gugaggagga guguggaguu cgccaagaag     1020

aggccaguug gagaccccuu cgaugccaaa acggagcagg ggccucagau cgaccaaaag     1080

caguuugaca aaauccucga gcugauugag agugggaaga aggaaggggc caagcuagaa     1140

uguggggggu cagccaugga ggacagaggg cuguucauca aacccacggu cuucucagau     1200

guuacggaca acaugaggau ugccaaagag gagauuuucg gaccagugca gccgauccug     1260

aaguucaaaa accuggagga ggugaucaag agagcgaaua gcaccgacua uggacucaca     1320

gcagcagugu ucaccaaaaa ccuggacaaa gcacugaagc uggcugcugc gcucgagucg     1380

gggacagucu ggaucaacug cuacaaugca uuuuaugcac aggcuccauu ugguggcuuc     1440

aaaaugucug ggaauggcag agaacuagga gaauaugcuc uggcugaaua uacagaagug     1500

aaaacuguca ccaucaaacu cgaggagaag aaccccuga                            1539


<210>  4
<211>  501
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ser Ser Ser Gly Thr Pro Asp Leu Pro Val Leu Leu Thr Asp Leu 
1               5                   10                  15      


Lys Ile Gln Tyr Thr Lys Ile Phe Ile Asn Asn Glu Trp His Asp Ser 
            20                  25                  30          


Val Ser Gly Lys Lys Phe Pro Val Phe Asn Pro Ala Thr Glu Glu Glu 
        35                  40                  45              


Leu Cys Gln Val Glu Glu Gly Asp Lys Glu Asp Val Asp Lys Ala Val 
    50                  55                  60                  


Lys Ala Ala Arg Gln Ala Phe Gln Ile Gly Ser Pro Trp Arg Thr Met 
65                  70                  75                  80  


Asp Ala Ser Glu Arg Gly Arg Leu Leu Tyr Lys Leu Ala Asp Leu Ile 
                85                  90                  95      


Glu Arg Asp Arg Leu Leu Leu Ala Thr Met Glu Ser Met Asn Gly Gly 
            100                 105                 110         


Lys Leu Tyr Ser Asn Ala Tyr Leu Asn Asp Leu Ala Gly Cys Ile Lys 
        115                 120                 125             


Thr Leu Arg Tyr Cys Ala Gly Trp Ala Asp Lys Ile Gln Gly Arg Thr 
    130                 135                 140                 


Ile Pro Ile Asp Gly Asn Phe Phe Thr Tyr Thr Arg His Glu Pro Ile 
145                 150                 155                 160 


Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro Leu Val Met Leu 
                165                 170                 175     


Ile Trp Lys Ile Gly Pro Ala Leu Ser Cys Gly Asn Thr Val Val Val 
            180                 185                 190         


Lys Pro Ala Glu Gln Thr Pro Leu Thr Ala Leu His Val Ala Ser Leu 
        195                 200                 205             


Ile Lys Glu Ala Gly Phe Pro Pro Gly Val Val Asn Ile Val Pro Gly 
    210                 215                 220                 


Tyr Gly Pro Thr Ala Gly Ala Ala Ile Ser Ser His Met Asp Ile Asp 
225                 230                 235                 240 


Lys Val Ala Phe Thr Gly Ser Thr Glu Val Gly Lys Leu Ile Lys Glu 
                245                 250                 255     


Ala Ala Gly Lys Ser Asn Leu Lys Arg Val Thr Leu Glu Leu Gly Gly 
            260                 265                 270         


Lys Ser Pro Cys Ile Val Leu Ala Asp Ala Asp Leu Asp Asn Ala Val 
        275                 280                 285             


Glu Phe Ala His His Gly Val Phe Tyr His Gln Gly Gln Cys Cys Ile 
    290                 295                 300                 


Ala Ala Ser Arg Ile Phe Val Glu Glu Ser Ile Tyr Asp Glu Phe Val 
305                 310                 315                 320 


Arg Arg Ser Val Glu Arg Ala Lys Lys Tyr Ile Leu Gly Asn Pro Leu 
                325                 330                 335     


Thr Pro Gly Val Thr Gln Gly Pro Gln Ile Asp Lys Glu Gln Tyr Asp 
            340                 345                 350         


Lys Ile Leu Asp Leu Ile Glu Ser Gly Lys Lys Glu Gly Ala Lys Leu 
        355                 360                 365             


Glu Cys Gly Gly Gly Pro Trp Gly Asn Lys Gly Tyr Phe Val Gln Pro 
    370                 375                 380                 


Thr Val Phe Ser Asn Val Thr Asp Glu Met Arg Ile Ala Lys Glu Glu 
385                 390                 395                 400 


Ile Phe Gly Pro Val Gln Gln Ile Met Lys Phe Lys Ser Leu Asp Asp 
                405                 410                 415     


Val Ile Lys Arg Ala Asn Asn Thr Phe Tyr Gly Leu Ser Ala Gly Val 
            420                 425                 430         


Phe Thr Lys Asp Ile Asp Lys Ala Ile Thr Ile Ser Ser Ala Leu Gln 
        435                 440                 445             


Ala Gly Thr Val Trp Val Asn Cys Tyr Gly Val Val Ser Ala Gln Cys 
    450                 455                 460                 


Pro Phe Gly Gly Phe Lys Met Ser Gly Asn Gly Arg Glu Leu Gly Glu 
465                 470                 475                 480 


Tyr Gly Phe His Glu Tyr Thr Glu Val Lys Thr Val Thr Val Lys Ile 
                485                 490                 495     


Ser Gln Lys Asn Ser 
            500     


<210>  5
<211>  518
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Thr Ser Ser Lys Ile Glu Met Pro Gly Glu Val Lys Ala Asp Pro 
1               5                   10                  15      


Ala Ala Leu Met Ala Ser Leu His Leu Leu Pro Ser Pro Thr Pro Asn 
            20                  25                  30          


Leu Glu Ile Lys Tyr Thr Lys Ile Phe Ile Asn Asn Glu Trp Gln Asn 
        35                  40                  45              


Ser Glu Ser Gly Arg Val Phe Pro Val Tyr Asn Pro Ala Thr Gly Glu 
    50                  55                  60                  


Gln Val Cys Glu Val Gln Glu Ala Asp Lys Ala Asp Ile Asp Lys Ala 
65                  70                  75                  80  


Val Gln Ala Ala Arg Leu Ala Phe Ser Leu Gly Ser Val Trp Arg Arg 
                85                  90                  95      


Met Asp Ala Ser Glu Arg Gly Arg Leu Leu Asp Lys Leu Ala Asp Leu 
            100                 105                 110         


Val Glu Arg Asp Arg Ala Val Leu Ala Thr Met Glu Ser Leu Asn Gly 
        115                 120                 125             


Gly Lys Pro Phe Leu Gln Ala Phe Tyr Val Asp Leu Gln Gly Val Ile 
    130                 135                 140                 


Lys Thr Phe Arg Tyr Tyr Ala Gly Trp Ala Asp Lys Ile His Gly Met 
145                 150                 155                 160 


Thr Ile Pro Val Asp Gly Asp Tyr Phe Thr Phe Thr Arg His Glu Pro 
                165                 170                 175     


Ile Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro Leu Leu Met 
            180                 185                 190         


Phe Ala Trp Lys Ile Ala Pro Ala Leu Cys Cys Gly Asn Thr Val Val 
        195                 200                 205             


Ile Lys Pro Ala Glu Gln Thr Pro Leu Ser Ala Leu Tyr Met Gly Ala 
    210                 215                 220                 


Leu Ile Lys Glu Ala Gly Phe Pro Pro Gly Val Ile Asn Ile Leu Pro 
225                 230                 235                 240 


Gly Tyr Gly Pro Thr Ala Gly Ala Ala Ile Ala Ser His Ile Gly Ile 
                245                 250                 255     


Asp Lys Ile Ala Phe Thr Gly Ser Thr Glu Val Gly Lys Leu Ile Gln 
            260                 265                 270         


Glu Ala Ala Gly Arg Ser Asn Leu Lys Arg Val Thr Leu Glu Leu Gly 
        275                 280                 285             


Gly Lys Ser Pro Asn Ile Ile Phe Ala Asp Ala Asp Leu Asp Tyr Ala 
    290                 295                 300                 


Val Glu Gln Ala His Gln Gly Val Phe Phe Asn Gln Gly Gln Cys Cys 
305                 310                 315                 320 


Thr Ala Gly Ser Arg Ile Phe Val Glu Glu Ser Ile Tyr Glu Glu Phe 
                325                 330                 335     


Val Arg Arg Ser Val Glu Arg Ala Lys Arg Arg Val Val Gly Ser Pro 
            340                 345                 350         


Phe Asp Pro Thr Thr Glu Gln Gly Pro Gln Ile Asp Lys Lys Gln Tyr 
        355                 360                 365             


Asn Lys Ile Leu Glu Leu Ile Gln Ser Gly Val Ala Glu Gly Ala Lys 
    370                 375                 380                 


Leu Glu Cys Gly Gly Lys Gly Leu Gly Arg Lys Gly Phe Phe Ile Glu 
385                 390                 395                 400 


Pro Thr Val Phe Ser Asn Val Thr Asp Asp Met Arg Ile Ala Lys Glu 
                405                 410                 415     


Glu Ile Phe Gly Pro Val Gln Glu Ile Leu Arg Phe Lys Thr Met Asp 
            420                 425                 430         


Glu Val Ile Glu Arg Ala Asn Asn Ser Asp Phe Gly Leu Val Ala Ala 
        435                 440                 445             


Val Phe Thr Asn Asp Ile Asn Lys Ala Leu Thr Val Ser Ser Ala Met 
    450                 455                 460                 


Gln Ala Gly Thr Val Trp Ile Asn Cys Tyr Asn Ala Leu Asn Ala Gln 
465                 470                 475                 480 


Ser Pro Phe Gly Gly Phe Lys Met Ser Gly Asn Gly Arg Glu Met Gly 
                485                 490                 495     


Glu Phe Gly Leu Arg Glu Tyr Ser Glu Val Lys Thr Val Thr Val Lys 
            500                 505                 510         


Ile Pro Gln Lys Asn Ser 
        515             


<210>  6
<211>  512
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Ala Thr Ala Asn Gly Ala Val Glu Asn Gly Gln Pro Asp Arg Lys 
1               5                   10                  15      


Pro Pro Ala Leu Pro Arg Pro Ile Arg Asn Leu Glu Val Lys Phe Thr 
            20                  25                  30          


Lys Ile Phe Ile Asn Asn Glu Trp His Glu Ser Lys Ser Gly Lys Lys 
        35                  40                  45              


Phe Ala Thr Cys Asn Pro Ser Thr Arg Glu Gln Ile Cys Glu Val Glu 
    50                  55                  60                  


Glu Gly Asp Lys Pro Asp Val Asp Lys Ala Val Glu Ala Ala Gln Val 
65                  70                  75                  80  


Ala Phe Gln Arg Gly Ser Pro Trp Arg Arg Leu Asp Ala Leu Ser Arg 
                85                  90                  95      


Gly Arg Leu Leu His Gln Leu Ala Asp Leu Val Glu Arg Asp Arg Ala 
            100                 105                 110         


Thr Leu Ala Ala Leu Glu Thr Met Asp Thr Gly Lys Pro Phe Leu His 
        115                 120                 125             


Ala Phe Phe Ile Asp Leu Glu Gly Cys Ile Arg Thr Leu Arg Tyr Phe 
    130                 135                 140                 


Ala Gly Trp Ala Asp Lys Ile Gln Gly Lys Thr Ile Pro Thr Asp Asp 
145                 150                 155                 160 


Asn Val Val Cys Phe Thr Arg His Glu Pro Ile Gly Val Cys Gly Ala 
                165                 170                 175     


Ile Thr Pro Trp Asn Phe Pro Leu Leu Met Leu Val Trp Lys Leu Ala 
            180                 185                 190         


Pro Ala Leu Cys Cys Gly Asn Thr Met Val Leu Lys Pro Ala Glu Gln 
        195                 200                 205             


Thr Pro Leu Thr Ala Leu Tyr Leu Gly Ser Leu Ile Lys Glu Ala Gly 
    210                 215                 220                 


Phe Pro Pro Gly Val Val Asn Ile Val Pro Gly Phe Gly Pro Thr Val 
225                 230                 235                 240 


Gly Ala Ala Ile Ser Ser His Pro Gln Ile Asn Lys Ile Ala Phe Thr 
                245                 250                 255     


Gly Ser Thr Glu Val Gly Lys Leu Val Lys Glu Ala Ala Ser Arg Ser 
            260                 265                 270         


Asn Leu Lys Arg Val Thr Leu Glu Leu Gly Gly Lys Asn Pro Cys Ile 
        275                 280                 285             


Val Cys Ala Asp Ala Asp Leu Asp Leu Ala Val Glu Cys Ala His Gln 
    290                 295                 300                 


Gly Val Phe Phe Asn Gln Gly Gln Cys Cys Thr Ala Ala Ser Arg Val 
305                 310                 315                 320 


Phe Val Glu Glu Gln Val Tyr Ser Glu Phe Val Arg Arg Ser Val Glu 
                325                 330                 335     


Tyr Ala Lys Lys Arg Pro Val Gly Asp Pro Phe Asp Val Lys Thr Glu 
            340                 345                 350         


Gln Gly Pro Gln Ile Asp Gln Lys Gln Phe Asp Lys Ile Leu Glu Leu 
        355                 360                 365             


Ile Glu Ser Gly Lys Lys Glu Gly Ala Lys Leu Glu Cys Gly Gly Ser 
    370                 375                 380                 


Ala Met Glu Asp Lys Gly Leu Phe Ile Lys Pro Thr Val Phe Ser Glu 
385                 390                 395                 400 


Val Thr Asp Asn Met Arg Ile Ala Lys Glu Glu Ile Phe Gly Pro Val 
                405                 410                 415     


Gln Pro Ile Leu Lys Phe Lys Ser Ile Glu Glu Val Ile Lys Arg Ala 
            420                 425                 430         


Asn Ser Thr Asp Tyr Gly Leu Thr Ala Ala Val Phe Thr Lys Asn Leu 
        435                 440                 445             


Asp Lys Ala Leu Lys Leu Ala Ser Ala Leu Glu Ser Gly Thr Val Trp 
    450                 455                 460                 


Ile Asn Cys Tyr Asn Ala Leu Tyr Ala Gln Ala Pro Phe Gly Gly Phe 
465                 470                 475                 480 


Lys Met Ser Gly Asn Gly Arg Glu Leu Gly Glu Tyr Ala Leu Ala Glu 
                485                 490                 495     


Tyr Thr Glu Val Lys Thr Val Thr Ile Lys Leu Gly Asp Lys Asn Pro 
            500                 505                 510         


